Services in Oral Care
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1 Services in Oral Care S1
2 Company Profile Vivacell Biotechnology GmbH is a privately owned contractbased research organisation (CRO) that provides specialised in vitro and in vivo models to test and develop pharmaceuticals, nutraceuticals, phytopharmaceuticals and health care products. VivaCell s preclinical services include standard protocols next to protocols individually adapted to customers needs using a large variety of in vitro and in vivo models. VivaCell is combining the latest know-how on cellular and molecular biology and best expertise with focus on oral care, cosmetics, immunology, CNS, nutraceuticals and natural products. VivaCell provides the complete range of project development from basic R&D up to pre-clinical research (GLP) in one hand. Our clients have the unique possibility to collect all relevant research data from one single source. VivaCell s expertise has been demonstrated in a substantial number of research projects and scientific publications. We have a successful track record over 10 years in oral health care product research and development S2
3 Services in Oral Care Oral care products are mostly used to prevent the oral cavity from caries, gingivitis and periodontitis. Gingivitis is caused by gum accumulating bacteria and ends up in red, swollen and bleeding inflammation of the gum. Rarely it caused by injuring the gums from overvigorous brushing. The daily use of selectively enriched oral care products like toothpaste or mouth rinses is the most effiective way to prevent gingivitis. VivaCell offers highly specialised and reliable models for testing oral care products (tooth paste, mouth wash etc.) for their potential to prevent or treat gingivitis. The models are used for testing marketed products as well as for the identification of new active principles and development of new products. VivaCell has a well established cooperation in this field with leading oral health care companies. S3
4 Services in Oral Health Care (in vitro) VivaCell offers the full service to assess prophylactic and acute therapeutic effects of oral care products All cellular models may be used for testing single chemical identities or diverse galenic formulations. We offer the use of special filter application to assess the effects of final formulations- simulating a real live application with prophylactic short time or acute treatment protocols. VivaCells provides the following models and methods: Human primary cultures: with monocytes, gingival and periodontal ligament fibroblasts and keratinocytes to determine all relevant inflammation parameters linked to gingivitis and periodontitis (prostaglandins, cytokines, NF-kappaB, free radicals, etc.). Capsaicin and menthol receptors (TPRV-1 and CMR1) heterologous transfected cells (artificial tongue) suitable to test new, topically applied analgesic compounds in oral care. Epigenetics: mirna, DNA methylation, HDACs Cell proliferation and cell cycle analysis: MTT, Alamar Blue, ATP, FACS. Phagocytosis: fluorescent beads by FACS Lectin binding assay: Studying the effect of ingredients on lecting binding to avoid bacterial adherence. S4
5 Services in Oral Health Care (in vitro) VivaCells furthermore provides: Wound healing and chemotaxis: cell migration by scratch assay or insert migration asssay, chemotaxis by Boyden chamber, real-time wound healing assays. Fibroblast cell line transfected with NF-kappaB promoter (luciferase assay). Intracellular signalling: kinases (MAPKs, Akt, PI3J, IkappaB etc.) by Western blot/elisa; transcription factors by luciferase assay (AP-1, CREB etc.). intracellular calcium by FACS or microscope. MMPs and TIMPs. Immunohistochemistry and histopathology, videomicroscopy, immunfluorescence, 3D-modelling. Mitochondrial function: respirometry, mitochondrial membrane potential, ATP levels, ROS, anti-oxidant enzymes etc. In development (available this summer): A novel platform to screen the effects of oral care products or ingredients on several important biolgical parameters for oral care. The capacity to develop new models according the requirements of our costumer. S4
6 Services in Oral Health Care Tissue models In addition to various cellular models, VivaCell offers highly specialised tissue models to simulate the in vivo situation. Tissue models may be used to test single compounds as well as galenic formulations. Gingival epithelial and buccal epithelial tissue Effects on human gingival fibroblast/ human keratinocytes co-cultures/3d culture (all parameters) hu-keratinocytes hu-gingival fibroblasts Determination of transepithelial electrical resistance By determination of the transepethelial electrical resistance, we offer to study the effects of compounds and galenic formulations on tight junctions. Determination of inflammatory parameters in gingival samples from clinical studies We offer the determination of inflammatory parameters PGE 2, IL-1, IL-8 and other cytokines, MMPs, free radicals (isoprostane) in various biological materials or samples from clinical studies. S5
7 Services in Oral Health Care Anti-bacterial activity (in cooperation) Haemophilus actinomycetemcomitans (aerob) Streptoccocus mutans Lactobacillus Actinobacillus actinomycetemcomitans (anaerob) Porphyromonas gingivalis (anaerob) Prevotella intermedia (anaerob) Safety studies (in cooperation, GLP and non-glp) Cytotoxicity Genetic and Mutagenic Toxicology Corrosion and Skin Irritation Skin Penetration Bioavailability For more information, please contact Dr. Bernd Fiebich VivaCell Biotechnology GmbH Ferdinand-Porsche Str. 5 D Denzlingen M: T: fiebich@vivacell.de S6
We prove the. health benefits, biological activity. and safety
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