Title:Pyrosequencing of supra- and subgingival biofilms from inflamed peri-implant and periodontal sites

Size: px
Start display at page:

Download "Title:Pyrosequencing of supra- and subgingival biofilms from inflamed peri-implant and periodontal sites"

Transcription

1 Author's response to reviews Title:Pyrosequencing of supra- and subgingival biofilms from inflamed peri-implant and periodontal sites Authors: Joerg Eberhard Simone Schaumann Ingmar Staufenbiel Ralph Scherer Markus Schilhabel Andreas Winkel Sascha N Stumpp (stumpp.nico@mh-hannover.de) Meike Stiesch (stiesch.meike@mh-hannover.de) Version:2Date:24 October 2014 Author's response to reviews: see over

2 MHH, Zahnärztliche Prothetik, OE 7710, Hannover Zentrum Zahn-, Mund- und Kieferheilkunde Klinik für Zahnärztliche Prothetik und Biomedizinische Werkstoffkunde Editor-in-Chief BMC Oral Health Professor Magdalena Morawska Prof. Dr. Jörg Eberhard, MMEd Telefon: Fax: Carl-Neuberg-Straße Hannover Telefon: Ihr Zeichen Ihre Nachricht vom Unser Zeichen Dear Editor-in-Chief, Dear Magdalena Morawska, Please find attached our revised manuscript entitled Pyrosequencing of supra- and subgingival biofilms from inflamed peri-implant and periodontal sites that we hope now is suitable for publication in the Journal BMC Oral Health. We are grateful to the reviewers comments and are the opinion that the manuscript has substantially improved now. Please find below our answers to the reviewers comments. With regards Jörg Eberhard

3 Reviewer's report Title:Pyrosequencing of supra- and subgingival biofilms in peri-implantitis and periodontitis lesions Version:1Date:25 September 2014 Reviewer: purnima kumar Reviewer's report: There are two major problems with this manuscript: 1. The definitions of disease are extremely loose. For example, the authors have used probe depths and bleeding on probing to select cases for sampling. These two criteria define a number of disease states and cannot definitively establish what the disease state was. Answer: The definition of the cases has been corrected. The manuscript has been renamed and the text in the Materials and Methods section has been adapted (p.:4; l.:2-11) so that there are no longer any definition problems. We have current X-rays that show evident radiographic bone loss. This information has been added to the text (p.:4; l.:7, 12-13). 2. Greengenes is capable of resolution at the genus level, not species. The authors have used this level for making comparisons. There are several species within each genus that are health compatible and disease associated. The genus Streptococcus is a prime example. At the genus level, S. mutans and S. mitis will fall into the same clade, but one causes disease and the other is beneficial. Answer: Because of the complexity of the expected microbiomes, the evaluation was performed at the genus level. The number of cases also suggests that analysis at the genus level is more reasonable than at the species level. Several current publications (Bizzarro S. (2013), Hu Y.J. (2013)) also deal with these problems at the genus level. The justification for this approach has been extended in the text (p.:12; l.:4), with additional literature. Others:Please spell check the document. Quality of written English: Needs some language corrections before being published Answer: The manuscript has been edited by a native English speaker with a background in the biological sciences. Reviewer's report Title: Pyrosequencing of supra- and subgingival biofilms in peri-implantitis and periodontitis lesions

4 Version:1Date:18 September 2014 Reviewer: Ana Paula Colombo Reviewer's report: Dear authors, This study aimed to compare the microbial composition of the supra and sub-gingival biofilm of teeth with periodontitis and implants with peri-implantitis. The authors concluded that the composition of the microbiota of both clinical diseases is similar although they are different diseases. The purpose of the study is interesting but there are several problems with writing and methodology that needs to be addressed. 1- The whole manuscript needs to be revised for typing errors and misspelling of bacteria names. Answer: The manuscript has been edited by a native English speaker with a background in the biological sciences. The spelling of the bacteria has been checked and corrected throughout the manuscript : Background: the sentence on lanes needs to be rephrased because Kumar did look at periodontitis and failing implants, although was the subgingival plaque. Answer: The sentence has been corrected, as recommended by the reviewer (p.:3; l.:11+12). 2.2: In fact, was really necessary to evaluate the supragingival plaque of both clinical diseases? What is the rationale? Answer: The aim of the study was to examine and compare the different microbiomes on implants and teeth, in order to be able to evaluate the significance of this as an etiological factor. For this reason, the authors considered that supragingival plaque should be included in the investigation : Lane 22: for the first time should be removed. This is very relative since a paper may come out tomorrow presenting similar methodology. Answer: The reviewer's recommendation has been followed and for the first time has been removed (p.:3; l.:21). 3.2: Remove the sentence on lanes 24-25, this is not the reason why you use the deep sequencing technique. Answer: This recommendation has been followed. 3.3: This whole paragraph should be improved, it is hard to follow. The sentence was invented should also be changed. Answer: This recommendation has been followed. The paragraph has been deleted (p.:3; l.:24ff)

5 4- Methods: smoking is a major risk factor for periodontitis and peri-implantitis, so no smoking within the last 6 months does not make sense. Smoking has a long and cumulative effect on the periodontal tissues. I believe that smoking should be excluded. Former smokers should have stopped for at least 5 years. Answer: The reviewer is right. Smokers have been excluded. The misleading sentence in Materials and Methods has been reformulated (p.:4; l.:10). 5- For a patient to have periodontitis or peri-implantitis, he/she had to have at least 1 site with PPD>4mm? So I could have a patient with several sites with periodontitis or periimplantitis and others with just one diseased site? This is important because measures the extension of disease. Diseased sites are reservoirs for periodontal pathogens that may cross-disseminate to other sites in the mouth. Answer: The missing data has been added in the Materials and Methods section and in Table 1 (p.:4; l.:2). 6- Clinical examination: for the plaque index API add a reference. Answer: The source has been added. 7- Sample collection: how did you collect supragingival plaque with paper points????? All samples in my opinion should have been taken with curettes. Answer: We used Paper Points Iso 30, which were stable enough to collect plaque. Thus, we could use the same technique for supra- and subgingival or supra- and submucosal. 8- DNA isolation, lane 15: sulcus fluid or subgingival plaque??? Answer: As recommended by the reviewer, text changed to for sampling (p.:5; l.:14). 9- How were the concentrations of DNA determined? Answer: The procedure has been added to the manuscript (p.:6+7; l.:25, 1-3). 10-My main criticism is related to the number of patients. Due to the high inter-individual variability of the periodontal microbiota, make conclusion and assumptions with 7 patients is problematic.

6 Answer: As the microbiome was expected to be complex, the evaluation was performed at the level of the genus. The number of cases is an additional reason to perform the analysis at the genus level rather than at the species level. Several current publications (Bizzarro S. (2013), Hu Y.J. (2013)) also study the problem at the level of the genus. The justification in the text has been extended (p.:12; l.:4) and additional literature has been cited : Results: table 1, there is something wrong with the plaque index % of implants? Answer: The numerical error has been corrected. 11.2: The description of the micro data should be improved, it is very confusing. Answer: The sentence structure has been changed to make this section clearer (p.:9; l.:9, 14, 18, 21). 12-What was the reason to do the Gram analysis? How was that done? The subtitle is not self-explanatory, the authors should have mentioned this in the methods. Answer: The Gram classification was intended to be an additional phenotypic classification. At this level, the data could be compared with other publications, e.g. Kumar et. al. (2012). Adjusted in the Materials and Methode section (p.:8; l.:1-2). 13-All figure legends should be improved, including details of the statistical analysis. Answer: All figure legends have been improved. The name of the bacteria in fig 1 should be clearer. What does c stand for? Answer: The quality of the figure has been improved, as well as the legend. 14-Discussion is too long and could be shortened. Answer: The reviewer's recommendation has been followed by deleting sections in the discussion. 15-Other major criticism here was the difficult in providing identification at the species level. This may be one of the reasons the authors found very similar microbiotas between the 2 groups or areas. Answer: One result of the study is indeed that the microbiome is very similar at the level of the genus.

7 16-Why do you think the classical periodontal pathogens were rarely detected and in low numbers? Answer: This sentence was removed when the discussion was abbreviated. 17-On lane 7, page 13: several studies (including Haffajee s studies) have shown that diversity is actually associated with health. Loss of diversity may lead to disease. Discuss that. Answer: Our study did not include healthy and diseased implants, so we are unable to contribute to this discussion. We have deleated the respective sentence in the discussion. 18-The whole discussion about Gram-positive and negative species does not seem relevant. Answer: This section was substantially shortened. The authors are the opinion that the discussion about Gram-positive and Gram-negative staining is relevant for the reader (see comment no. 12). 19-The authors should be careful with the conclusions saying the there are no major differences between the microbiota of periodontitis and peri-implantitis based on the analysis of 7 patients. Answer: The conclusion section has been rephrased according to the reviewer s suggestions (p.:14; l.:15-18). 20-All references need to be reviewed, the order, the names of the journals and some of them are incomplete. Answer: The references have been checked and corrected. Quality of written English: Needs some language corrections before being published Answer: The manuscript has been checked by a native English speaker with a background in the biological sciences. Reviewer's report Title: Pyrosequencing of supra- and subgingival biofilms in peri-implantitis and

8 periodontitis lesions Version:1Date:24 September 2014 Reviewer:Anna Louropoulou Reviewer's report: In the section M&M, line 12-13; the diagnosis of peri-implantitis and periodontitis is based on BOPand PD. The diagnosis of peri-implantitis and periodontitis is based also on the presence of radiographic bone loss. If radiographic data are not provided the use of terms periimplantitis or periodontitis can be misleading. There is a chance that some of the cases included are gingivitis or mucositis cases. The authors should provide radiographic data if they want to continue using these terms otherwise, if radiographic data are not available, they should use other terminology. Answer: We have followed the reviewer's recommendation, see Commentary 1 for Reviewer 1. Level of interest: An article whose findings are important to those with closely related research interests Quality of written English: Acceptable Statistical review: Yes, but I do not feel adequately qualified to assess the statistics. Declaration of competing interests: I declare that I have no competing interests

Title:Video-confidence: a qualitative exploration of videoconferencing for psychiatric emergencies

Title:Video-confidence: a qualitative exploration of videoconferencing for psychiatric emergencies Author's response to reviews Title:Video-confidence: a qualitative exploration of videoconferencing for psychiatric emergencies Authors: Marianne V Trondsen (marianne.trondsen@telemed.no) Stein Roald Bolle

More information

Peri-implantitis Review

Peri-implantitis Review 1 2 Aro n J. Saf f er D DS M S Di pl omate o f th e Ame ric an Bo ard of P eri od o n tol og y A quarterly review of the latest publications related to the study of Peri-implant inflammation and bone loss

More information

Title: Prevalence of sexual, physical and emotional abuse in the Norwegian Mother and Child Cohort Study

Title: Prevalence of sexual, physical and emotional abuse in the Norwegian Mother and Child Cohort Study Author's response to reviews Title: Prevalence of sexual, physical and emotional abuse in the Norwegian Mother and Child Cohort Study Authors: Marie F Sorbo (marie.flem.sorbo@ntnu.no) Hilde Grimstad (hilde.grimstad@ntnu.no)

More information

Author s response to reviews

Author s response to reviews Author s response to reviews Title: Comparison of VividTrac, Airtraq, King Vision, Macintosh Laryngoscope and a Custom-Made Videolaryngoscope for Difficult and Normal Airways in Mannequins by Novices Authors:

More information

Title: Human chorionic gonadotropin and its relation to grade, stage and patient survival in ovarian cancer

Title: Human chorionic gonadotropin and its relation to grade, stage and patient survival in ovarian cancer Author's response to reviews Title: Human chorionic gonadotropin and its relation to grade, stage and patient survival in ovarian cancer Authors: Miriam Lenhard (miriam.lenhard@med.uni-muenchen.de) Alexandra

More information

Title: A survey of attitudes toward clinical research among physicians at Kyoto University Hospital

Title: A survey of attitudes toward clinical research among physicians at Kyoto University Hospital Author's response to reviews Title: A survey of attitudes toward clinical research among physicians at Kyoto University Hospital Authors: Eriko Sumi (sumieri@kuhp.kyoto-u.ac.jp) Toshinori Murayama (murayama@kuhp.kyoto-u.ac.jp)

More information

Title:Environmental footprints of Mediterranean versus Western Dietary Patterns: Beyond the health benefits of the Mediterranean Diet

Title:Environmental footprints of Mediterranean versus Western Dietary Patterns: Beyond the health benefits of the Mediterranean Diet Author's response to reviews Title:Environmental footprints of Mediterranean versus Western Dietary Patterns: Beyond the health benefits of the Mediterranean Diet Authors: Sara Sáez Almendros (sara.saez.almendros@gmail.com)

More information

Title: Protocol-based management of older adults with hip fractures in Delhi, India: a feasibility study

Title: Protocol-based management of older adults with hip fractures in Delhi, India: a feasibility study Reviewer s report Title: Protocol-based management of older adults with hip fractures in Delhi, India: a feasibility study Version: 0 Date: 16 Nov 2015 Reviewer: Cristin Ryan Reviewer's report: This paper

More information

Persson GR, Salvi GE, Heitz-Mayfield LJA et al. Antimicrobial therapy using a local drug delivery system (Arestin) in the treatment of

Persson GR, Salvi GE, Heitz-Mayfield LJA et al. Antimicrobial therapy using a local drug delivery system (Arestin) in the treatment of Persson GR, Salvi GE, Heitz-Mayfield LJA et al. Antimicrobial therapy using a local drug delivery system (Arestin) in the treatment of peri-implantitis I: microbiological outcomes. Clin Oral Imp Res 2006;

More information

Title: Quality of life in childhood epilepsy with lateralized focus

Title: Quality of life in childhood epilepsy with lateralized focus Author's response to reviews Title: Quality of life in childhood epilepsy with lateralized focus Authors: Krystyna A. Mathiak (krystyna.mathiak@psych.uw.edu.pl) Malgorzata Luba (malgosia.luba@gmail.com)

More information

Title: Socioeconomic conditions and number of pain sites in women

Title: Socioeconomic conditions and number of pain sites in women Author's response to reviews Title: Socioeconomic conditions and number of pain sites in women Authors: Finn E Skjeldestad (fisk@fhi.no) Toril Rannestad (Toril.Rannestad@hist.no) Version: 2 Date: 17 January

More information

Title: Use of food labels by adolescents to make healthier choices on snacks: a cross sectional study from Sri Lanka

Title: Use of food labels by adolescents to make healthier choices on snacks: a cross sectional study from Sri Lanka Author s response to reviews Title: Use of food labels by adolescents to make healthier choices on snacks: a cross sectional study from Sri Lanka Authors: Ishanka Talagala (drmaheshkeerthi@gmail.com;drishanka@gmail.com)

More information

Title: Survival endpoints in colorectal cancer. The effect of second primary other cancer on disease free survival.

Title: Survival endpoints in colorectal cancer. The effect of second primary other cancer on disease free survival. Author's response to reviews Title: Survival endpoints in colorectal cancer. The effect of second primary other cancer on disease free survival. Authors: Helgi Birgisson (helgi.birgisson@surgsci.uu.se)

More information

Author's response to reviews

Author's response to reviews Author's response to reviews Title: Corticosteroid co-treatment induces resistance to chemotherapy in surgical resections, xenografts, and established cell lines of pancreatic cancer Authors: Chengwen

More information

Title:Continuity of GP care is associated with lower use of complementary and alternative medical providers A population-based cross-sectional survey

Title:Continuity of GP care is associated with lower use of complementary and alternative medical providers A population-based cross-sectional survey Author's response to reviews Title:Continuity of GP care is associated with lower use of complementary and alternative medical providers A population-based cross-sectional survey Authors: Anne Helen Hansen

More information

FACULTY OF HEALTH SCIENCES INSTITUTE OF CLINICAL DENTISTRY

FACULTY OF HEALTH SCIENCES INSTITUTE OF CLINICAL DENTISTRY Professor Gottfried Schmalz Editor-in-Chief Clinical Oral Investigations Universität Regensburg Fakultät für Medizin Poliklinik für Zahnerhatungskunde und Parodontologie Frans-Josef-Strauss-Allee 11 93053

More information

Title: Epidemiological and virological investigation of a Norovirus outbreak in a resort in Puglia, Italy

Title: Epidemiological and virological investigation of a Norovirus outbreak in a resort in Puglia, Italy Author's response to reviews Title: Epidemiological and virological investigation of a Norovirus outbreak in a resort in Puglia, Italy Authors: Caterina Rizzo (caterina.rizzo@iss.it) Ilaria Di Bartolo

More information

APA Writing Style. Advanced Workshop

APA Writing Style. Advanced Workshop APA Writing Style Advanced Workshop Objectives This workshop is intended to cover some of the more advanced topics within the Publication Manual of the American Psychological Association, 6th edition.

More information

Title: Home Exposure to Arabian Incense (Bakhour) and Asthma Symptoms in Children: A Community Survey in Two Regions in Oman

Title: Home Exposure to Arabian Incense (Bakhour) and Asthma Symptoms in Children: A Community Survey in Two Regions in Oman Author's response to reviews Title: Home Exposure to Arabian Incense (Bakhour) and Asthma Symptoms in Children: A Community Survey in Two Regions in Oman Authors: Omar A Al-Rawas (orawas@squ.edu.om) Abdullah

More information

Title: Non-small cell lung carcinoma in an adolescent, manifested by acute paraplegia due to spinal metastases - case report

Title: Non-small cell lung carcinoma in an adolescent, manifested by acute paraplegia due to spinal metastases - case report Author's response to reviews Title: Non-small cell lung carcinoma in an adolescent, manifested by acute paraplegia due to spinal metastases - case report Authors: Ulrike Ackert (ulrike_ackert@hotmail.com)

More information

Title: Seroprevalence of Human Papillomavirus Types 6, 11, 16 and 18 in Chinese Women

Title: Seroprevalence of Human Papillomavirus Types 6, 11, 16 and 18 in Chinese Women Author's response to reviews Title: Seroprevalence of Human Papillomavirus Types 6, 11, 16 and 18 in Chinese Women Authors: Jia Ji (jackie.j.ji@gmail.com) He Wang (hewangpeking@gmail.com) Jennifer S. Smith

More information

Periodontal Maintenance

Periodontal Maintenance Periodontal Maintenance Friday, February 20, 2015 1:06 PM Periodontal disease control always begins with patient education - Plaque control, diet, smoking cessation, impact that systemic health has on

More information

Title:Effectiveness of a quality management program in dental care practices

Title:Effectiveness of a quality management program in dental care practices Author's response to reviews Title:Effectiveness of a quality management program in dental care practices Authors: Katja Goetz (katja.goetz@med.uni-heidelberg.de) Stephen M Campbell (stephen.campbell@manchester.ac.uk)

More information

Title: Dengue Score: a proposed diagnostic predictor of pleural effusion and/or ascites in adult with dengue infection

Title: Dengue Score: a proposed diagnostic predictor of pleural effusion and/or ascites in adult with dengue infection Reviewer s report Title: Dengue Score: a proposed diagnostic predictor of pleural effusion and/or ascites in adult with dengue infection Version: 0 Date: 11 Feb 2016 Reviewer: Anthony Jin Shun Chua Reviewer's

More information

RISK FACTORS FOR PERIODONTITIS

RISK FACTORS FOR PERIODONTITIS RISK FACTORS FOR PERIODONTITIS Brian A. Burt, BDS, MPH, PhD School of Public Health and School of Dentistry University of Michigan Ann Arbor, Michigan ESSENTIALS OF EPIDEMIOLOGY Groups rather than individuals

More information

Title: Peri-articular tranexamic acid injection in total knee arthroplasty: A Randomized controlled trial

Title: Peri-articular tranexamic acid injection in total knee arthroplasty: A Randomized controlled trial Author s response to reviews Title: Peri-articular tranexamic acid injection in total knee arthroplasty: A Randomized controlled trial Authors: Piya Pinsornsak (Pinpiya2003@yahoo.com) Santi Rojanavijitkul

More information

Author's response to reviews

Author's response to reviews Author's response to reviews Title:Differences upon admission and in hospital course of children hospitalized pneumonia: a retrospective cohort study Authors: Raquel Simbalista (r.simbalista@terra.com.br)

More information

Faculty of Dentistry PERIODONTICS 1429 /1430

Faculty of Dentistry PERIODONTICS 1429 /1430 Faculty of Dentistry ORAL BASIC AND CLINICAL SCIENCES DEPARTMENT Course Portfolio PERIODONTICS Course Number PERIO 401 O.B.C.S 445 1429 /1430 2008/ 2009 Course Syllabus CONTENTS: 1- INSTRUCTOR INFORMATION

More information

Materials and Methods: Literature review and Authors opinion.

Materials and Methods: Literature review and Authors opinion. Haffajee AD, Bogren A, Hasturk H et al. Subgingival microbiota of chronic periodontitis subjects from different geographic locations. J Clin Periodontol 2004; 31:996-1002. Purpose: To compare the subgingival

More information

EFFECTIVE MEDICAL WRITING Michelle Biros, MS, MD Editor-in -Chief Academic Emergency Medicine

EFFECTIVE MEDICAL WRITING Michelle Biros, MS, MD Editor-in -Chief Academic Emergency Medicine EFFECTIVE MEDICAL WRITING Michelle Biros, MS, MD Editor-in -Chief Academic Emergency Medicine Why We Write To disseminate information To share ideas, discoveries, and perspectives to a broader audience

More information

Title: Response to M. tuberculosis selected RD1 peptides in Ugandan HIV-infected patients with smear positive pulmonary tuberculosis: a pilot study

Title: Response to M. tuberculosis selected RD1 peptides in Ugandan HIV-infected patients with smear positive pulmonary tuberculosis: a pilot study Author's response to reviews Title: Response to M. tuberculosis selected RD1 peptides in Ugandan HIV-infected patients with smear positive pulmonary tuberculosis: a pilot study Authors: Delia Goletti (d.goletti@tiscali.it)

More information

Author's response to reviews

Author's response to reviews Author's response to reviews Title: A multicentre, double-blind, randomised, controlled, parallel-group study of the effectiveness of a pharmacist-acquired medication history in an emergency department

More information

# BMJ R1 entitled "The role of the gut microbiome in nutrition and health"

# BMJ R1 entitled The role of the gut microbiome in nutrition and health 19-Mar-2018 Dear Dr. Valdes # BMJ.2017.040712.R1 entitled "The role of the gut microbiome in nutrition and health" Thank you for sending us this commissioned paper and for your patience while we have been

More information

Clinpro Glycine Prophy Powder

Clinpro Glycine Prophy Powder Clinpro Glycine Prophy Powder 3M 2016. All Rights Reserved VIDEO 3M 2016. All Rights Reserved 2 Presentation Overview Air Polishing Procedure With Clinpro Glyciene Prophy Powder Use and Efficiency for

More information

Title: In vitro tooth whitening effect of two medicated chewing gums compared to a whitening gum and saliva

Title: In vitro tooth whitening effect of two medicated chewing gums compared to a whitening gum and saliva Author's response to reviews Title: In vitro tooth whitening effect of two medicated chewing gums compared to a whitening gum and saliva Authors: Michael Moore (moorem@ipfw.edu) Nathalie Hasler-Nguyen

More information

11 September Tower Old Broad Street London EC2N 1HQ United Kingdom t + 44 (0) f + 44 (0)

11 September Tower Old Broad Street London EC2N 1HQ United Kingdom t + 44 (0) f + 44 (0) 11 September 2007 Jim Sylph International Auditing and Assurance Standards Board 545 5th Avenue, 14th Floor New York, New York 10017 USA Edcomments@ifac.org Tower 42 25 Old Broad Street London EC2N 1HQ

More information

Title: Aggrecan heterogeneity in articular cartilage from patients with osteoarthritis

Title: Aggrecan heterogeneity in articular cartilage from patients with osteoarthritis Reviewer s report Title: Aggrecan heterogeneity in articular cartilage from patients with osteoarthritis Version: 0 Date: 21 Dec 2015 Reviewer: Debabrata Patra Reviewer s report: In this article the authors

More information

Author's response to reviews

Author's response to reviews Author's response to reviews Title:A Randomised Multicentre Trial of Acupuncture in Patients with Seasonal Allergic Rhinitis - Study Intervention including Physician and Treatment Characteristics Authors:

More information

PEER REVIEW HISTORY ARTICLE DETAILS TITLE (PROVISIONAL)

PEER REVIEW HISTORY ARTICLE DETAILS TITLE (PROVISIONAL) PEER REVIEW HISTORY BMJ Open publishes all reviews undertaken for accepted manuscripts. Reviewers are asked to complete a checklist review form (http://bmjopen.bmj.com/site/about/resources/checklist.pdf)

More information

Title: Identifying work ability promoting factors for home care aides and assistant nurses

Title: Identifying work ability promoting factors for home care aides and assistant nurses Author's response to reviews Title: Identifying work ability promoting factors for home care aides and assistant nurses Authors: Agneta Larsson (agneta.larsson@ltu.se) Lena Karlqvist (lena.karlqvist@ltu.se)

More information

Report to the editors of the journal

Report to the editors of the journal Report to the editors of the journal I would first like to thank you, madam / sir, for this collaboration with our journal Interventions économiques / Papers in Political Economy. Those invited to do a

More information

PEER REVIEW HISTORY ARTICLE DETAILS VERSION 1 - REVIEW. Ball State University

PEER REVIEW HISTORY ARTICLE DETAILS VERSION 1 - REVIEW. Ball State University PEER REVIEW HISTORY BMJ Open publishes all reviews undertaken for accepted manuscripts. Reviewers are asked to complete a checklist review form (see an example) and are provided with free text boxes to

More information

Mechanical Non Surgical Therapy: An Indispensable Tool

Mechanical Non Surgical Therapy: An Indispensable Tool IOSR Journal of Dental and Medical Sciences (JDMS) ISSN: 2279-0853, ISBN: 2279-0861. Volume 1, Issue 4 (Sep-Oct. 2012), PP 36-41 Mechanical Non Surgical Therapy: An Indispensable Tool 1 Ashu Bhardwaj,

More information

Title: Association of interleukin-33 and thymic stromal lymphopoietin levels with the development of idiopathic pulmonary fibrosis

Title: Association of interleukin-33 and thymic stromal lymphopoietin levels with the development of idiopathic pulmonary fibrosis Author s response to reviews Title: Upregulation of interleukin-33 and thymic stromal lymphopoietin levels in the lungs of idiopathic pulmonary fibrosis Authors: Jong-Uk Lee (lajoal@nate.com) Hun Soo Chang

More information

Author's response to reviews

Author's response to reviews Author's response to reviews Title: Wnt1 is epistatic to Id2 in modeling mammary gland development and in causing mammary tumors Authors: Susan Marino (smarino@cmgm.stanford.edu) Claire Romelfanger (clairefrog@yahoo.com)

More information

Title: The Limitations of Voluntary Medical Male Circumcision and the Importance of Sustained Condom Use: A Kenyan Newspaper Analysis

Title: The Limitations of Voluntary Medical Male Circumcision and the Importance of Sustained Condom Use: A Kenyan Newspaper Analysis Author's response to reviews Title: The Limitations of Voluntary Medical Male Circumcision and the Importance of Sustained Condom Use: A Kenyan Newspaper Analysis Authors: Charlene N Muzyka (charlene.muzyka@gmail.com)

More information

Show biofilm the red card

Show biofilm the red card Show biofilm the red card BIOFILM-ERASER 1 A NEW APPLICATION Effective treatment for periodontal and peri-implant inflammation Periodontitis and peri-implantitis are bacterial inflammations with similar

More information

Title:Problematic computer gaming, console-gaming, and internet use among adolescents: new measurement tool and association with time use

Title:Problematic computer gaming, console-gaming, and internet use among adolescents: new measurement tool and association with time use Author's response to reviews Title:Problematic computer gaming, console-gaming, and internet use among adolescents: new measurement tool and association with time use Authors: Mette Rasmussen (mera@niph.dk)

More information

Title: Influenza vaccination among healthcare workers in a multidisciplinary University hospital in Italy

Title: Influenza vaccination among healthcare workers in a multidisciplinary University hospital in Italy Author's response to reviews Title: Influenza vaccination among healthcare workers in a multidisciplinary University hospital in Italy Authors: Susanna Esposito (susanna.esposito@unimi.it) Samantha Bosis

More information

Please visit the C.E. Pavilion to validate your course attendance Or If There s a Line Go cdapresents.com

Please visit the C.E. Pavilion to validate your course attendance Or If There s a Line Go cdapresents.com UCLA Innovations 2016 CDA Presents in Anaheim Tara Aghaloo, DDS, MD, PhD Dean Ho, MS, PhD Jay Jayanetti Eric C. Sung, DDS David T. W. Wong, DMD, DMSc Benjamin M. Wu, DDS, PhD Saturday, May 14, 2016 8:00

More information

Title:The role of BRCA1 and BRCA2 mutations in prostate, pancreatic and stomach cancers.

Title:The role of BRCA1 and BRCA2 mutations in prostate, pancreatic and stomach cancers. Author's response to reviews Title:The role of BRCA1 and BRCA2 mutations in prostate, pancreatic and Authors: Helen Cavanagh (helen21987@hotmail.com) Katherine MA Rogers (k.rogers@qub.ac.uk) Version:3Date:1

More information

Maintenance in the Periodontally Compromised Patient. Dr. Van Vagianos January 22, 2009 Charlotte Dental Hygiene Study Club

Maintenance in the Periodontally Compromised Patient. Dr. Van Vagianos January 22, 2009 Charlotte Dental Hygiene Study Club Maintenance in the Periodontally Compromised Patient Dr. Van Vagianos January 22, 2009 Charlotte Dental Hygiene Study Club Periodontal Maintenance for Natural Teeth and Implants What is Periodontal Maintenance?

More information

Title:Association of resting heart rate with cardiovascular function: a cross-sectional study in 522 Finnish subjects

Title:Association of resting heart rate with cardiovascular function: a cross-sectional study in 522 Finnish subjects Author's response to reviews Title:Association of resting heart rate with cardiovascular function: a cross-sectional study in 522 Finnish subjects Authors: Jenni K Koskela (jenni.k.koskela@uta.fi) Anna

More information

RESPONSE TO DECISION LETTER

RESPONSE TO DECISION LETTER RESPONSE TO DECISION LETTER Dear Editor-in-chief, We are grateful to the editors and reviewers for their time and constructive comments on our manuscript. We have implemented their comments and suggestions

More information

Bacterial Plaque and Its Relation to Dental Diseases. As a hygienist it is important to stress the importance of good oral hygiene and

Bacterial Plaque and Its Relation to Dental Diseases. As a hygienist it is important to stress the importance of good oral hygiene and Melissa Rudzinski Preventive Dentistry Shaunda Clark November 2013 Bacterial Plaque and Its Relation to Dental Diseases As a hygienist it is important to stress the importance of good oral hygiene and

More information

PEER REVIEW HISTORY ARTICLE DETAILS. Zou, Yuming; Li, Quan; Xu, Weidong VERSION 1 - REVIEW

PEER REVIEW HISTORY ARTICLE DETAILS. Zou, Yuming; Li, Quan; Xu, Weidong VERSION 1 - REVIEW PEER REVIEW HISTORY BMJ Open publishes all reviews undertaken for accepted manuscripts. Reviewers are asked to complete a checklist review form (http://bmjopen.bmj.com/site/about/resources/checklist.pdf)

More information

Title: Overlap of Cognitive Concepts in Chronic Widespread Pain: An Exploratory Study

Title: Overlap of Cognitive Concepts in Chronic Widespread Pain: An Exploratory Study Author's response to reviews Title: Overlap of Cognitive Concepts in Chronic Widespread Pain: An Exploratory Study Authors: Aleid de Rooij (a.d.rooij@reade.nl) Martijn P.M. Steultjens (martijn.steultjens@gcal.ac.uk)

More information

Title: The impact of the UK 'Act FAST' stroke awareness campaign: content analysis of patients, witness and primary care clinicians' perceptions

Title: The impact of the UK 'Act FAST' stroke awareness campaign: content analysis of patients, witness and primary care clinicians' perceptions Author's response to reviews Title: The impact of the UK 'Act FAST' stroke awareness campaign: content analysis of patients, witness and primary care clinicians' perceptions Authors: Stephan U Dombrowski

More information

Title: A Central Storage Facility to Reduce Pesticide Suicides- A Feasibility Study from India

Title: A Central Storage Facility to Reduce Pesticide Suicides- A Feasibility Study from India Author's response to reviews Title: A Central Storage Facility to Reduce Pesticide Suicides- A Feasibility Study from India Authors: Lakshmi Vijayakumar (lakshmi@vijayakumars.com) Jeyaseelan Lakshmanan

More information

Title:Postpartum contraceptive use in Gondar town, Northwest Ethiopia: a community based cross-sectional study

Title:Postpartum contraceptive use in Gondar town, Northwest Ethiopia: a community based cross-sectional study Author's response to reviews Title:Postpartum contraceptive use in Gondar town, Northwest Ethiopia: a community based cross-sectional study Authors: Yeshewas Abera (yeshiab57@gmail.com) Zelalem Birhanu

More information

Title: TIMP-1 and VEGF-165 serum concentration during first-line therapy of ovarian cancer patients

Title: TIMP-1 and VEGF-165 serum concentration during first-line therapy of ovarian cancer patients Author's response to reviews Title: TIMP-1 and VEGF-165 serum concentration during first-line therapy of ovarian cancer patients Authors: Sven Mahner (Sven.Mahner@gmx.de) Linn Woelber (lwoelber@uke.uni-hamburg.de)

More information

Author's response to reviews

Author's response to reviews Author's response to reviews Title: Validation of Doloplus-2 among nonverbal nursing home patients - An evaluation of Doloplus-2 in a clinical setting An evaluating of Doloplus-2 in a clinical setting

More information

Department of Forest Ecosystems and Society, Oregon State University

Department of Forest Ecosystems and Society, Oregon State University July 4, 2018 Prof. Christopher Still Department of Forest Ecosystems and Society, Oregon State University 321 Richardson Hall, Corvallis OR 97331-5752 Dear Prof. Christopher Still, We would like to thank

More information

Reviewer s report. Version: 0 Date: 11 Apr Reviewer: Ruth Kipping. Reviewer's report:

Reviewer s report. Version: 0 Date: 11 Apr Reviewer: Ruth Kipping. Reviewer's report: Reviewer s report Title: Translating an early childhood obesity prevention program for local community implementation: A Case Study of the Melbourne InFANT Program Version: 0 Date: 11 Apr 2016 Reviewer:

More information

Jose Merino (Chair), Georg Roeggla, Tiago Villaneuva, John Fletcher. Amy Price, Elisabeth Loder. Jamie Kirhham (statisticians), Rubin Minhas

Jose Merino (Chair), Georg Roeggla, Tiago Villaneuva, John Fletcher. Amy Price, Elisabeth Loder. Jamie Kirhham (statisticians), Rubin Minhas BMJ.2017.037871 "Efficacy of drugs used in preventive chemotherapy against soil-transmitted helminths: systematic review and network meta-analysis" *********************************************************************************************************

More information

Author's response to reviews

Author's response to reviews Author's response to reviews Title:Does the 2013 GOLD classification improve the ability to predict lung function decline, exacerbations and mortality? A post-hoc analysis of the 4-year UPLIFT trial Authors:

More information

Title: The effect of Breast Cancer Awareness Month on Internet search activity - a comparison with awareness campaigns for lung and prostate cancer

Title: The effect of Breast Cancer Awareness Month on Internet search activity - a comparison with awareness campaigns for lung and prostate cancer Author's response to reviews Title: The effect of Breast Cancer Awareness Month on Internet search activity - a comparison with awareness campaigns for lung and prostate cancer Authors: Ronan W Glynn (ronanglynn@doctors.net.uk)

More information

Title:Emergency ambulance service involvement with residential care homes in the support of older people with dementia: an observational study

Title:Emergency ambulance service involvement with residential care homes in the support of older people with dementia: an observational study Author's response to reviews Title:Emergency ambulance service involvement with residential care homes in the support of older people with dementia: an observational study Authors: Sarah Amador (s.amador@herts.ac.uk)

More information

Title:Hyperphosphatemia as an Independent Risk Factor of Coronary Artery Calcification Progression in Peritoneal Dialysis Patients

Title:Hyperphosphatemia as an Independent Risk Factor of Coronary Artery Calcification Progression in Peritoneal Dialysis Patients Author's response to reviews Title:Hyperphosphatemia as an Independent Risk Factor of Coronary Artery Calcification Progression in Peritoneal Dialysis Patients Authors: Da Shang (sdshangda@163.com) Qionghong

More information

Periodontal Treatment Protocol Department of Orthodontics and Restorative Dentistry, Glenfield Hospital, Leicester

Periodontal Treatment Protocol Department of Orthodontics and Restorative Dentistry, Glenfield Hospital, Leicester Periodontal Treatment Protocol Department of Orthodontics and Restorative Dentistry, Glenfield Hospital, Leicester 1. Periodontal Assessment Signs of perio disease: - Gingivae become red/purple - Gingivae

More information

Title: Exploring approaches to patient safety: The case of spinal manipulation therapy

Title: Exploring approaches to patient safety: The case of spinal manipulation therapy Reviewer s report Title: Exploring approaches to patient safety: The case of spinal manipulation therapy Version: 0 Date: 15 Feb 2016 Reviewer: Duncan Reid Reviewer's report: BCAM-D-16-00036 Re-thinking

More information

Title: Intention-to-treat and transparency of related practices in randomized, controlled trials of anti-infectives

Title: Intention-to-treat and transparency of related practices in randomized, controlled trials of anti-infectives Author s response to reviews Title: Intention-to-treat and transparency of related practices in randomized, controlled trials of anti-infectives Authors: Robert Beckett (rdbeckett@manchester.edu) Kathryn

More information

Title: Associations between depression and different measures of obesity (BMI, WC, WHtR, WHR)

Title: Associations between depression and different measures of obesity (BMI, WC, WHtR, WHR) Author's response to reviews Title: Associations between depression and different measures of obesity (BMI, WC, WHtR, WHR) Authors: Jörg Wiltink (joerg.wiltink@unimedizin-mainz.de) Matthias Michal (matthias.michal@unimedizin-mainz.de)

More information

# BMJ entitled " Complete the antibiotic course to avoid resistance ; non-evidence-based dogma which has run its course?

# BMJ entitled  Complete the antibiotic course to avoid resistance ; non-evidence-based dogma which has run its course? Dear Dr. Llewelyn # BMJ.2017.037542 entitled " Complete the antibiotic course to avoid resistance ; non-evidence-based dogma which has run its course?" Thank you for sending us this paper and giving us

More information

Title:Prevalence of binge drinking and associated behaviours among 3286 college students in France

Title:Prevalence of binge drinking and associated behaviours among 3286 college students in France Author's response to reviews Title:Prevalence of binge drinking and associated behaviours among 3286 college students in France Authors: Marie-Pierre Tavolacci (marie-pierre.tavolacci@chu-rouen.fr) Eloise

More information

Body: Re Should the threshold for definition of impaired fasting glucose be lowered?

Body: Re Should the threshold for definition of impaired fasting glucose be lowered? From: dme_editorial@wiley.com To: inouek@m.u-tokyo.ac.jp CC: dme_editorial@wiley.com Subject: Diabetic Medicine DME-2009-00292 Body: Re Should the threshold for definition of impaired fasting glucose be

More information

Title: High muscular fitness has a powerful protective cardiometabolic effect in adults: Influence of weight status

Title: High muscular fitness has a powerful protective cardiometabolic effect in adults: Influence of weight status Author s response to reviews Title: High muscular fitness has a powerful protective cardiometabolic effect in adults: Influence of weight status Authors: Robinson Ramírez-Vélez (robin640@hotmail.com) Jorge

More information

Title:Modern contraceptive use among sexually active men in Uganda: Does discussion with a health worker matter?

Title:Modern contraceptive use among sexually active men in Uganda: Does discussion with a health worker matter? Author's response to reviews Title:Modern contraceptive use among sexually active men in Uganda: Does discussion with a health worker matter? Authors: Allen Kabagenyi Ms. (allenka79@yahoo.com) Patricia

More information

Global Harmonization Task Force SG3 Comments and Recommendations ISO/DIS 9001: 2000 and ISO/DIS 9000: 2000 And Revision of ISO and 13488

Global Harmonization Task Force SG3 Comments and Recommendations ISO/DIS 9001: 2000 and ISO/DIS 9000: 2000 And Revision of ISO and 13488 Page 1 of 6 Global Harmonization Task Force SG3 ISO/DIS 9001: 2000 and ISO/DIS 9000: 2000 And Revision of ISO 13485 and 13488 GENERAL COMMENTS The Global Harmonization Task Force Study Group Three (GHTF

More information

Straumann SmartOne. Stage 4 Af terc are and maintenance. Step 2 Maintenance visit

Straumann SmartOne. Stage 4 Af terc are and maintenance. Step 2 Maintenance visit Stage 4 Af terc are and maintenance Step 2 Maintenance visit Overview Assessment and treatment planning Step 1 Patient's expectations, history and examination Step 2 Treatment planning Step 3 Consultation

More information

Implantology without Periodontology is like Yin without Yang.

Implantology without Periodontology is like Yin without Yang. Implantology without Periodontology is like Yin without Yang. NIWOP: The systematic and evidence-based workflow #niwop wh.com No Implantology without Periodontology NIWOP 1 Pretreatment Optimal periodontal

More information

Title:Mixed-strain Housing for Female C57BL/6, DBA/2, and BALB/c Mice: Validating a Split-plot Design that promotes Refinement and Reduction

Title:Mixed-strain Housing for Female C57BL/6, DBA/2, and BALB/c Mice: Validating a Split-plot Design that promotes Refinement and Reduction Author's response to reviews Title:Mixed-strain Housing for Female C57BL/6, DBA/2, and BALB/c Mice: Validating a Split-plot Design that promotes Refinement and Reduction Authors: Michael Walker Mr (mwalk04@uoguelph.ca)

More information

Title: Increased incidence of traffic accidents in RhD-negative, Toxoplasma gondii-infected military drivers revealed by a prospective cohort study

Title: Increased incidence of traffic accidents in RhD-negative, Toxoplasma gondii-infected military drivers revealed by a prospective cohort study Author's response to reviews Title: Increased incidence of traffic accidents in RhD-negative, Toxoplasma gondii-infected military drivers revealed by a prospective cohort study Authors: Jaroslav Flegr

More information

Fighting Gum Disease: A Special Report

Fighting Gum Disease: A Special Report Fighting Gum Disease: A Special Report Note: Nothing in this book should be construed as advice. Though I have made every attempt to make sure the information is correct and accurate, I cannot guarantee

More information

Title:Does Osteoporosis reduce the primary tilting stability of cementless acetabular cups?

Title:Does Osteoporosis reduce the primary tilting stability of cementless acetabular cups? Author's response to reviews Authors: Christoph von Schulze Pellengahr (C.pellengahr@arcor.de) Lars V von Engelhardt (larsvictor@gmx.de) Bernd Wegener (Bernd.Wegener@med.uni-muenchen.de) Peter E Müller

More information

Dental Water Jet. By: Jennifer Buffington, Lindsay Hunt, Ruth Gardner

Dental Water Jet. By: Jennifer Buffington, Lindsay Hunt, Ruth Gardner Dental Water Jet By: Jennifer Buffington, Lindsay Hunt, Ruth Gardner What is a Dental Water Jet? A home, dental self-care device that uses a stream of pulsating water to irrigate the subgingival sulcus

More information

Title: What 'outliers' tell us about missed opportunities for TB control: a cross-sectional study of patients in Mumbai, India

Title: What 'outliers' tell us about missed opportunities for TB control: a cross-sectional study of patients in Mumbai, India Author's response to reviews Title: What 'outliers' tell us about missed opportunities for TB control: a cross-sectional study of patients in Authors: Anagha Pradhan (anp1002004@yahoo.com) Karina Kielmann

More information

Title:The role of BRCA1 and BRCA2 mutations in prostate, pancreatic and stomach cancers.

Title:The role of BRCA1 and BRCA2 mutations in prostate, pancreatic and stomach cancers. Author's response to reviews Title:The role of BRCA1 and BRCA2 mutations in prostate, pancreatic and stomach cancers. Authors: Helen Cavanagh (helen21987@hotmail.com) Katherine MA Rogers (k.rogers@qub.ac.uk)

More information

Title: Sports activity and combined use of snus and cigarette smoking among young males in Finland in

Title: Sports activity and combined use of snus and cigarette smoking among young males in Finland in Author's response to reviews Title: Sports activity and combined use of snus and cigarette smoking among young males in Finland in 1999-2010 Authors: Ville M Mattila (ville.mattila@uta.fi) Susanna Raisamo

More information

Author's response to reviews

Author's response to reviews Author's response to reviews Title: Gender and age differences among current smokers in a general population survey Authors: Ulrich John (ujohn@uni-greifswald.de) Monika Hanke (hanke@uni-greifswald.de)

More information

Title: A Prospective Study of Dietary Selenium Intake and Risk of Type 2 Diabetes

Title: A Prospective Study of Dietary Selenium Intake and Risk of Type 2 Diabetes Author's response to reviews Title: A Prospective Study of Dietary Selenium Intake and Risk of Type 2 Diabetes Authors: Saverio Stranges (S.Stranges@warwick.ac.uk) Sabina Sieri (Sabina.Sieri@istitutotumori.mi.it)

More information

Title:Bounding the Per-Protocol Effect in Randomized Trials: An Application to Colorectal Cancer Screening

Title:Bounding the Per-Protocol Effect in Randomized Trials: An Application to Colorectal Cancer Screening Author's response to reviews Title:Bounding the Per-Protocol Effect in Randomized Trials: An Application to Colorectal Cancer Screening Authors: Sonja A Swanson (sswanson@hsph.harvard.edu) Oyvind Holme

More information

Author's response to reviews

Author's response to reviews Author's response to reviews Title: Diabetes duration and health-related quality of life in individuals with onset of diabetes in the age group 15-34 years - a Swedish population-based study using EQ-5D

More information

Course Title: DH 118 A Clinical Dental Hygiene I-Lecture Term: Fall

Course Title: DH 118 A Clinical Dental Hygiene I-Lecture Term: Fall Course Objective 1. Relate the history of dental hygiene to the concept of preventive dentistry and describe the dental hygienist's scope of practice. 1.1. Relate the history of the dental hygiene profession

More information

Title: Estimation of the burden of varicella in Europe before the introduction of universal childhood immunization

Title: Estimation of the burden of varicella in Europe before the introduction of universal childhood immunization Author s response to reviews Title: Estimation of the burden of varicella in Europe before the introduction of universal childhood immunization Authors: Margarita Riera-Montes (margarita.riera@p-95.com)

More information

National Examining Board for Dental Nurses. NEBDN National Diploma in Dental Nursing

National Examining Board for Dental Nurses. NEBDN National Diploma in Dental Nursing NEBDN National Diploma in Dental Nursing Information on the Written Examination Format NEBDN has developed new assessment criteria for use in the NEBDN Diploma in Dental Nursing examination. This is completely

More information

In addition, we have asked an English-editing service to edit the text, and you will find an English-edited version of the paper submitted as well.

In addition, we have asked an English-editing service to edit the text, and you will find an English-edited version of the paper submitted as well. Author s response to reviews Title: Resource Use and Disease Course in Dementia - Nursing Home (REDIC-NH): A Norwegian Cohort Study from Admission to a Nursing Home until Death. A description of study

More information

RE: Title: Practical fecal calprotectin cut-off value for Japanese patients with ulcerative colitis

RE: Title: Practical fecal calprotectin cut-off value for Japanese patients with ulcerative colitis September 10, 2018 Professor Xue-Jiao Wang, MD Science Editor Editorial Office 'World Journal of Gastroenterology' RE: 40814 Title: Practical fecal calprotectin cut-off value for Japanese patients with

More information

Title: Risser Patient Satisfaction Scale: A Validation study in Greek Cancer Patients

Title: Risser Patient Satisfaction Scale: A Validation study in Greek Cancer Patients Author's response to reviews Title: Risser Patient Satisfaction Scale: A Validation study in Greek Cancer Patients Authors: Andreas I Charalambous (andreas.charalambous@cut.ac.cy) Theodoula Adamakidou

More information

Title: Body fatness and breast cancer risk in women of African ancestry

Title: Body fatness and breast cancer risk in women of African ancestry Author's response to reviews Title: Body fatness and breast cancer risk in women of African ancestry Authors: Elisa V Bandera (elisa.bandera@rutgers.edu) Urmila Chandran (chandrur@cinj.rutgers.edu) Gary

More information