SKIN HISTOLOGY the microscopic anatomy of the Integument. Mikrogeo. com

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1 SKIN HISTOLOGY the microscopic anatomy of the Integument Mikrogeo. com

2 Hair follicles, sweat glands, sebaceous glands (even teeth) are products of the epidermis,embryologically speaking ectododerm, that extend downward into the dermis.

3 Palms & soles thick skin 3.Stratum Granulosum 4.Stratum Lucidum 5.Stratum Corneum Note Keratin Granules 1.Stratum Germinativum 2.Stratum Spinosum Note intercellular bridges or desmosomes in S. Spinosum Dermis Note melanin granules in Melanocytes in S. Germinativum The 5 Layers of the Epidermis (1.5 um ultra thin H&E)

4 Cells start in 1.SG and pass up through into 5.SC to slough off Desmosomes or intercellular bridges between St. Sp. cells DERMIS dense irregular CT Intercellular Bridges ( Desmosomes Pigment or Melanin Granules in St. Germinativum, these melanocytes are where Melanomas arise in neoplastic growth, but dark color absorbs UV light ) in Stratum Spinosum

5 Stratum Corneum Stratum Spinosum Keratin Granules produced by St. Granulosum Cells; note how they become spindle-shaped & lose their nuclei

6 Right thumb Right index finger whorl Loop end Delta core Short seg. Type lines bifurcating Matching an individual s dermal ridge patterns in fingerprint analysis NO 2 are alike

7 Epidermis Sebaceous Gland Sweat Gl. Hair Follicle Dermis Sweat Gl. THIN SKIN Hair and sebaceous glands

8 Arrector Pili Muscle (think goosebumps ) Sweat Gland Hair Shaft Sebaceous Gland Hair Shaft Sebaceous Gland Sebaceous Gland Hair Shaft Arrector Pili Muscle DERMIS

9 Epidermis Hair Shaft Arrector Pili Muscle Hair Shaft Hair Shaft

10 Epidermis D E R M I S Arrector Pili Muscle Coiling Sweat Gland Duct

11 Meissner s corpuscletouch What Structures are these?

12 HAIR SHAFT Sebaceous Gland (if dirty = black head, if infected and with puss= white head ) Reverse of skin (peel) showing hairs & associated sebaceous glands

13 1 hair shaft and bulb Sweat Gland Blood Vessel

14 Cuticle of hair & inner root sheath Outer epithelial root sheath bulb Cortex of hair Huxley s layer Henley s layer Medulla in center of Cortex; together = hair shaft Plucked hair and bulb

15 Hair Shaft Broken frag. of epidermis Hair Bulb : full of DNA overlapping scales on surface of human hair Pulled hair

16 Root of Hair with its Papilla Bulb

17 Nail Bed Eponychium Hyponychium Distal Phalanx Epidermis Dermis Fat Pad End of Human Infant Finger

18 Nails and hair are dead keratin (protein) Left distal phalange of 5 th finger - total amputation Nail growth Lunule (site of nail growing stratum germinativum) below eponychium Skin is rapidly repaired

19 EPIDERMIS Irregular denser connective tissue Irregular denser connective tissue 2 Sweat Glands & Their Ducts Adipose or Fat cells

20 Human Axilla: Apocrine Sweat Glands (arm pit & crotch area) note copius secretion granule formation and Myoepithelium smooth muscle fibers (arrows) at base of each secretory cell solid Basement Membrane separates epithelial cells from all others Pieces of cell enter lumen, quickly attacked by odoriferous bacteria

21 Pressure sense Paccinian Corpuscles

22 Breasts & Teats Mammary glands Food for mammal young who stay with mother

23 Mammary Gland-Inactive Proliferative Human-Nipple ducts

24 sun Epidermis stratum basale/germinativum absorbs UV light Provitamin D Some reflection Vitamin D B.M. Dermis Melanin absorbs most UV UV Abused melanocytes of stratum basale/germinativum become cancerous & metastasize to body through blood & lymph= MELANOMA

25 My Squamous Cell Carcinoma - Thanks to Drs. Adams & Finkelstein

26 BV s cauterized 1.5 cm Biopsy 5/26/ 15 by Dr. Finkelstein; dermatopathologist diagnosis is squamous cell carcinoma; PDT (photodynamic therapy) done on 6/19/ 15 destroys most Actinic Keratosis (AK) a precancerous lesion. 1.5 cm

27 Area around excision where xylocaine injected 1.5 cm 1.4 cm 1 st Excision of Mohs surgery 7/1/ 15 Dr. Adams

28 1 hr. frozen section, H&E Stratum Corneum & St. Lucidum 1 hr.: frozen section, H&E at 400X Stratum Corneum - sloughing Epidemis Keratin of St. Granulosum Basement Memb. St. Basale/Germinativum Small amount of melanin gr. Intercellular Bridges of Stratum Spinosum Hair Follicle Mohs diagnosis: all cancer removed: normal tissue Dense irregular CT of DERMIS

29 Fatty (adipose) dermis Sebaceous Glands Hair & Follicle Sebaceous Glands gives me oily scalp&hair Sebaceous Glands 1 hr.: frozen section, H&E at 400X Mohs diagnosis: Dr. Will Adams - all cancer removed: all normal tissue, i.e. no squamous pearls surrounded by malignant cells with pleomorphic nuclei & mitotic figures.

30 T- shaped, enlarged incision to suture properly for scarless healing based on age, complexion; dermis sutured separately 1.5 cm. 1.8 cm Post Mohs Excision (2 hrs.) 2.6 cm

21 Skin and it s Accessories

21 Skin and it s Accessories 21 Skin and it s Accessories 21-001 Skin 1 2 Sweat glands Adipose tissue 3 Retinaculum cutis 21-01. Skin of the palm 1. General view. Human, H-E stain, x 3.3. Opening of sweat gland duct Stratum corneum

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