Enhanced Fundus Images by Using Hybrid Neighbourhood Estimator before Filling with Fuzzy Based Filtering
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1 Enhanced Fundus Images by Using Hybrid Neighbourhood Estimator before Filling with Fuzzy Based Filtering Manpreet Kaur M.Tech Scholar, CSE CGC COE Landran Mohali, India grover.manpreet523@gmail.com Jagbir Singh Gill Associate Professor, CSE CGC COE Landran Mohali,India cgccoe.jagbir@gmail.com Abstract: Diabetic Retinopathy is an eye disorder that leads to blindness caused by damage in retina. For this reason the exact recognition of vessels become difficult. In order to overcome this difficulty and early detection of eye diseases there are various vessel segmentation techniques. This paper presents a Hybrid Neighbourhood Estimator before Filling (NEBF) with fuzzy based filtering technique which enables us to segment vessels even in low intensity of images. We Firstly extracted the exudates from fundus image then applying NEBF. NEBF is an impainting filter which is used to inpaint exudates so that false positives are reduced in image. The fuzzy based filtering technique is applied based on segmentation. The proposed method is tested on DRIVE database. This provides us with better results over the existing methods even in the case when low depth of images. Keywords: Fundus images, NEBF, Fuzzy based filtering, Retinal Vessel Segmentation The main contribution of this paper is to use the fuzzy I. INTRODUCTION based filtering technique to improve the accuracy of vessel segmentation. We Segment image of retina in this way so that vessel part and non-vessel portions are divided from each other. Then from these segmented vessels automatic diagnosis of various diseases become easy [3]. Automated detection of retinopathy in eye fundus images using digital image analysis methods has more benefits which let the study of quite a few images faster, with lower cost. This automatic discovery of retinal vessels structure is an important in the improvement of automated framework for that scrutiny of vasculature distribution. So it will be very helpful to ophthalmologist to diagnosis the patient suffering from eyes. The manual detection of blood vessels is very difficult since the blood vessels in a retinal image are complicated in structure. The residue of the paper contains some sections which are as follows. Section II described the related work. Section III explained the material used. Section IV organized the proposed work. Section V represents Experimental Results. Section VI summarized the conclusion. Digital imaging utilizing a fundus camera is widely regarded an integral part of medical examination in ophthalmology. Retinal digital images usually are called Fundus images taken by digital fundus camera. Fundus picture contains a few essential parts as an optical disk, macula and body vascular, which are essential for correct examination by ophthalmologist. Retinal vessels have very important information regarding the condition of patient. From fundus images we find eye disorder. There is a great increase in number of people which are suffering from eye related disorders. Many common eye related diseases are glaucoma, diabetic retinopathy, and age-related macular degeneration. Because of diseases the chances of loss of perspective and blindness are very high [1]. There exist therapy that could stop the advancement of the illness and therefore timely recognition is crucial in order to avoid irreversible vision problems. The blood vessels in the fundus image must certainly be segmented and analyzed to get a notion of the illness affecting the attention like glaucoma and diabetic retinopathy. Segmentation is typically the first stage in virtually any attempt to analyze or understand an image automatically. Segmentation links the space between low-level image handling and high-level image processing. Some types of segmentation strategy will undoubtedly be found in any application relating to the detection, acceptance, and rating of things in images. The key motto of segmentation is to lessen the data for quick evaluation [2]. II. RELATED WORK Deepashree Devaraj (2014) [7] proposed a user-friendly graphical interface (GUI) MATLAB centered that segments the arteries and through median thresholding. From your segmented image, various options that come with arteries and like area, mean, entropy, standard deviation and histogram are determined, to have the capacity to realize the images as regular or abnormal. RES Publication 2012 Page 1
2 Pardeep Kaur (2015) [8] Thresholding shows a substantial position in image segmentation. Thresholding is used to locate an optimal threshold for the image. The GA assures the local optimization however, not assures the global optimization, the entire effect is dependent upon the choice of bad population may bring for bad segmentation. To overcome the limitation of the GA on the basis of the multilevel thresholding Ant Colony Optimization is applied. J. B.Sindhu, J.B.Jeeva (2013) [10] Review of retinal vessel is a crucial factor for your many medical disorders. The retinal vessel examination might be achieved beginning with extracting the retinal images from the background. The improvements inside retinal vessels on account of pathologies may be simply acquiesced by segmenting the retinal vessels. Authors have described the automated techniques for retinal vessel segmentation. Segmentation of retinal vessels is completed to identify early proper diagnosis of the infection like diabetic retinopathy, hypertensive retinopathy and arteriosclerosis. Vein is segmented using morphological operation while using disc designed structuring factor is used. This strategy is used to the overtly available DRIVE database. Mangaiarkarasi (2016) [11] Brain tumor is one of the very most hazardous infection occurs generally in human beings. MRI brain image strategy is widely used to measure and envision the brain's anatomical structure. The recognition of tumor in brain required several process on MRI image such as image enhancement, image processing, classification, binarization, image segmentation, image extraction, the last classification process determine that the individual is diseased or not. Authors described ant colony optimization (ACO) method which done segmentation of tumor in MRI images. Annunziata et al. (2016) [12] Recognition is created more difficult in pathological image with the well known items presence of exudates and other abnormalities. An unsupervised vessel segmentation approach is revealed to take care of this problem. A novel impainting filter, called Neighbourhood estimator before filling, is proposed to inpaint exudates in techniques local false positives are significantly reduced during vessel enhancement. Retinal general improvement is reached by using a multiple-scale Hessian approach. The proposed vessel segmentation technique outperforms state-ofthe-art algorithm noted in vehicles new literature, equally artistically and with regard to quantitative measurements. Muthu et al. (2012) [13] Proposed a technique to segment the fundus image by using AdaBoost. In this paper 41-D feature vector is done for every single pixel. These feature vector have information regarding the pixel's intensity, spatial qualities, and structural attributes of image at various scales. Then AdaBoost classifier is employed that establishes whether pixel belongs to vessel or non-vessel class.after literature survey, it has been originated that the majority of existing vessel segmentation techniques suffers from the many issues which preserving the edges but not so efficient for high density of multiple noises. The main contribution of this paper is to use fuzzy based filtering technique to improve the accuracy of vessel segmentation which enables us to segment vessels even in low intensity of images. III. MATERIAL USED A. Structure of the Human Eye Human eye is the light-sensitive organ that enables one to see the surrounding environment. The image is formed on the retina of eye. The image formed on the retina is transformed into electrical impulses and carried to the brain through the optic nerves, where the signals are processed and the sensation of vision is generated [4]. The general diagram of human eye is shown in Figure 1. B. Drive Dataset Figure1.Human eye [5] The vessel segmentation methodologies were evaluated by using publicly available database containing the retinal images. The DRIVE database contains 20 retinal color images. The images have been captured using Canon CR5 nonmydriatic 3-CCD camera with a 45 field of view (FOV). Each image has pixels with 8 bits per color channel in JPEG format. The database is divided into two groups: training set and test set, each containing 20 images. The training set contains color fundus images, the FOV masks for the images, and a set of manually segmented monochrome ground truth images. The test set contains color fundus images, the FOV masks for the images, and two set of manually segmented monochrome ground truth images [6]. RES Publication 2012 Page 2
3 IV. PROPOSED WORK Figure 2. Flow Chart of proposed Method A. Read Image Firstly we read the image i.e. we load the image into memory which we segment to get retinal vessels. Then before applying the techniques we normalized the image. B. Fuzzy Filtering The idea driving this filtering is usually to normal your pixel using additional pixel ideals by reviewing the neighborhood, however simultaneously to address critical image structures just like edges. The main priority from the recommended filtering is usually to distinguish between regional variations as a result of noises in addition to as a result of image structure. Your order to accomplish this, each pixel all of us derive a price in which communicates their education where the mixture within a particular path is usually small. This kind of a price is usually derived each path equivalent on the nearby pixels from the refined pixel by way of fluffy rule. The further engineering from the filtering is then in line with the watching with interest which a modest fluffy mixture almost certainly is usually the result of noises, whilst a sizable fluffy mixture almost certainly is usually the result of a footing while in the image. Therefore, each path i will use not one but two fluffy regulations in which carry this specific watching with interest note, understanding that decide this contribution from the nearby pixel values. The consequence of these regulations is usually de fuzzified plus a a static correction term is usually purchased for any refined pixel importance. C. Grey Scaling of Image Fundus color image is first converted into a grayscale/green-channel image to support the blood vessels segmentation and to reduce the computational time. Gray-scale gives just the luminance data along with picture following reducing the shade and saturation, because green-channel image gives optimum local contrast between the back ground and foreground. The segmentation results details are acquired using gray-channel image or g-indexed image. D. Neighbourhood Estimator Before filling (NEBF) NEBF is an impainting filter that will be applied to inpaint the exudates i.e. filling the holes in images \or restore lost or deteriorated part of image to ensure that false positives are reduced for vessel segmentation. NEBF is used to to make image clean before segmentation. In this we replace bad spots (exudates) with its neighbouring pixels so that it looks alike Neighbourhood. Firstly we consider region in image to impainted. Then start from the boundary of the region it takes a small Neighbourhood around the pixel on the neighborhood to be impainted [12]. These pixels are replaced by the normalized weighted sum of the entire known pixel in neighborhood. After impainting the boundary we go inside the region by filling everything in the boundary first. Sometimes, they are leaving a narrow border of undetected pixels. For this reason we use morphological filtering operations. The dilation and erosion morphological operations are applied iteratively for shrinking or expand the area to get the vessels. V. EXPERIMENTAL RESULTS Some well-known image performance evaluation parameters for digital images have been selected to prove that the performance of the proposed algorithm is quite better than the existing method. The calculated measures are sensitivity, positive predictive value, False Discovery Rate, F_measure and accuracy. Sensitivity (Se) are the ratios of correctly classified vessels and. While Positive predictive value (Ppv) is the ratio of pixels correctly classified as vessel pixel and False Discovery Rate (FDR) is the ratio of pixels correctly classified as background. Accuracy is measured as the ratio of correctly classified pixels to the total number of pixels in field of view (FOV). The function for calculating performance measures is implemented in Matlab code. A. Sensitivity It is also known as the correct positive rate or the recall or likelihood of detection. Sensitivity measures the amount of positives that are properly identified. Sensitivity is giving higher amount of positives in every case. Se = TP TP + FN RES Publication 2012 Page 3
4 B. F-measure Figure 3. Performance Analysis of Sensitivity F-measure views equally the precision p and the remember r. p is how many appropriate excellent results separated by how many all excellent results, and r is how many appropriate excellent results separated by how many excellent results which should have now been returned. precision. recall F_measure = 2. presision + recall Figure 5. Performance Analysis of Positive Predictive Value D. False Discovery Rate (FDR) FDR is also called True Negative. FDR measures the ratio of correctly classified non-vessel pixels. The test makes a negative prediction. FDR = FP FP + TP Figure 4. Performance Analysis of F-Measure Figure 6. Performance Analysis of False Discovery Rate C. Positive Predictive Value PPV is also called True Positive or Precision. PPV measures the ratio of correctly classified vessel pixels. PPV = TP TP + FP E. Accuracy Accuracy is the proportion of true results or the proximity of measurement results to the true value. Accuracy is used to describe the closeness of a measurement to the true value. TP + TN Accuracy = TP + TN + FP + FN RES Publication 2012 Page 4
5 Figure 7. Performance Analysis of Accuracy VI. CONCLUSION Retinal vessel segmentation main component is an exudates impainted using impainting filter aimed at reducing false detection generated by strong contrast around exudates. So it becomes very important that this segmentation should be carried out with great care. It represents a hybrid combination of Neighbourhood Estimator before Filling with fuzzy based filtering technique is implemented. This paper has planned the Hybrid Techniques which helps us to segment vessels even in low intensity of images. The evaluation of techniques has been performed by utilizing parameters like Sensitivity, Positive Predictive Value, FDR, F_measure and Accuracy shows the performance analysis by taking the DRIVE database but this proposed work can be tested on different database to be worked in future. REFERENCES [1] Lee, Noah, Andrew Laine, and R. Theodore Smith. "A hybrid segmentation approach for geographic atrophy in fundus autofluorescence images for diagnosis of age-related macular degeneration." In Engineering in Medicine and Biology Society, EMBS th Annual International Conference of the IEEE, pp IEEE, [2] Fang, Guoliang, Nan Yang, Huchuan Lu, and Kaisong Li. "Automatic segmentation of hard exudates in fundus images based on boosted soft segmentation." In Intelligent Control and Information Processing (ICICIP), 2010 International Conference on, pp IEEE, [3] Yin, Fengshou, Jiang Liu, SimHengOng, Ying Sun, Damon WK Wong, NganMeng Tan, Carol Cheung, Mani Baskaran, Tin Aung, and Tien Yin Wong. "Model-based optic nerve head segmentation on retinal fundus images." InEngineering in Medicine and Biology Society, EMBC, 2011 Annual International Conference of the IEEE, pp IEEE, [4] Human eye anatomy :http : // / resources/anatomy.htm ( ). [5] Human eye diagram: https: // nei.nih.gov /health/ eyediagram ( ) [6] [7] Deepashree Devaraj1, Dr. Prasanna Kumar S.C Blood Vessels segmentation with GUI in digital Fundus Images for Automated detection of Diabetic Retinopathy Contemporary Computing and Informatics (IC3I), 2014 International Conference. [8] Pardeep Kaur, Er. Neetu Gupta Image Segmentation using ant Colony Optimization and to Improved the Selection of Poor Segmentation International Journal for Advance Research in Engineering and Technology Volume 3, Issue VIII, Aug [9] Jiri Minar, MarekPinkava, KamilRiha, Malay Kishore Dutta, Anushikha Singh Automatic Extraction of Blood Vessels and Veins using Adaptive Filters in Fundus Image [10] B.Sindhu, J.B.Jeeva Automatic Retinal Vessel Segmentation Using Morphological Operation and Threshold International Journal of Scientific & Engineering Research, Volume 4, Issue 5, May [11] MANGAIARKARASI, MUTHULAKSHMI Medical Image Segmentation Using Ant Colony Optimization algorithm 2016 IJARMATE. [12] Roberto Annunziata*, Andrea Garzelli, Lucia Ballerini, Alessandro Mecocci, and EmanueleTrucco. Leveraging Multiscale Hessian-based Enhancement with a novel Exudate Inpainting technique for retinal Vessel Segmentation IEEE JOURNAL OF BIOMEDICAL AND HEALTH INFORMATICS. [13] Muthu Rama Krishnan, M., U. RajendraAcharya, Chua Kuang Chua, Lim Choo Min, Eddie Yin-Kwee Ng, Milind M. Mushrif, and Augustinus Laude. "Application of intuitionistic fuzzy histon segmentation for the automated detection of optic disc in digital fundus images." In Biomedical and Health Informatics (BHI), 2012 IEEE-EMBS International Conference on, pp IEEE, [14] Y. Tyagi1, T. A. Puntambekar1, Preeti Sexena2 and Sanjay Tanwani2, A Hybrid Approach to Edge Detection using Ant Colony Optimization and Fuzzy Logic, International Journal of Hybrid Information Technology Vol. 5, No. 1, January, AUTHOR S BIOGRAPHIES Manpreet Kaur is a M.tech Scholar in Department of Computer Science and Engineering, CGC Landran, Mohali. She received the Btech degree in computer science and Engineering from Chandigarh university, Gharuan, India in Her research interests include computer RES Publication 2012 Page 5
6 vision, image processing, pattern recognition, and medical Informatics. Jagbir Singh Gill was born in Ludhiana, India. He received the B.E. degree in Computer Science and Engineering from SLIET University Longowal, Punjab, India, and the M.Tech. Degrees in Computer science and engineering from the Guru Nanak Dev Engineering College Ludhiana, India, in He is pursuing Ph.D from PTU under Guidance of Dr. K S Mann. In 2012, he joined the as an Assistant Professor. His current research interests include Medical Informatics, Digital Image processing. He had published more than 20 Papers in International journals and 4 papers in International conferences RES Publication 2012 Page 6
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