MCP uL 25uL 25uL 25uL Typical Dilution 1:2 None None None Manufacturer Defined Minimum Detectible Concentration

Transcription

1 Units: pg/ml pg/ml pg/ml pg/ml Assay Name RnD Systems Millipore Magnetic Hormone ELISA Adipokine Panel 2 Panel Catalog Number DCP HADK2MAG-61K HCYTOMAG-6K HMHMAG-34K EGF, FGF-2, Eotaxin, TGF-a, G-CSF, Flt-3L, GM-CSF, Fractalkine, IFNa2, IFNy, GRO, IL-1, MCP-3, IL-12p4, MDC, IL-12p7, PDGF- Amylin (active), Amylin (total), C-Peptide, Other Analytes On IL-6, Insulin, Leptin, TNFa, HGF, IL-8, NGF, IL-1b N/A AA, IL-13, PDGF-BB, IL-15, scd4l, IL-17, IL1Ra, sil-2ra, IL-1a, IL- Ghrelin, GIP, Glucagon, IL-6, Insulin, Multiplex 9, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IP-1,, MIP-1a, Leptin,, PP, PYY, TNF-a MIP-1b, RANTES, TNF-a, TNF-b, VEGF Volume Required Per Replicate 25uL 25uL 25uL 25uL Typical Dilution 1:2 None None None Manufacturer Defined Minimum Detectible Concentration 3.4 pg/ml 1.2 pg/ml 1.9 pg/ml 6. pg/ml UVM Observed Minimum Detectible Concentration pg/ml 1.2 pg/ml 1.9 pg/ml 6. pg/ml UVM Run Date 6/9/215 4/16/213 7/31/215 1/9/215 SCS81611: SCS81611: SCS81611: SCS81611: SCS81611: SCS81611: SCS81611: SCS81611: SCS81611: SCS81611: SCS81611: SCS81611: SCS81611: SCS81611: SCS81611: SCS81611: SCS81611: SCS81611: SCS81611: SCS81611: Average Notes: Serum and EDTA are NOT sourced from the same pools. Each set is from unique pools/participants. Sample type correlations may differ due to a number of factors including; measured range of set, sample type variability, reagent lot variability, specimen condition, pre-analytical variation et. UVM Protocol for RnD ELISA is 1% extrapolation below lowest standard point. SCS = Serum Control Set

2 Serum : RnD ELISA vs. Millipore Magnetic Adipokine Panel y =.6214x R² = RnD ELISA Serum : RnD ELISA vs. Millipore Magnetic Metabolic 6 5 y =.5111x R² = RnD ELISA Serum : RnD ELISA vs. Millipore Magnetic Cytokine Panel 6 5 y = x R² = RnD ELISA

3 Serum : Millipore Magnetic Adipokine Panel vs. Magnetic Cytokine Panel 6 y = 2.547x R² =.8973 Serum : Millipore Magnetic Cytokine 6 y =.3677x R² = Serum : Millipore Magnetic Adipokine 6 y =.7999x R² =

4 Units: pg/ml pg/ml pg/ml pg/ml Assay Name RnD Systems Millipore Magnetic Hormone ELISA Adipokine Panel 2 Panel Catalog Number DCP HADK2MAG-61K HCYTOMAG-6K HMHMAG-34K EGF, FGF-2, Eotaxin, TGF-a, G-CSF, Flt-3L, GM-CSF, Fractalkine, IFNa2, IFNy, GRO, IL-1, MCP-3, IL-12p4, MDC, IL-12p7, PDGF- Amylin (active), Amylin (total), C-Peptide, Other Analytes On IL-6, Insulin, Leptin, TNFa, HGF, IL-8, NGF, IL-1b N/A AA, IL-13, PDGF-BB, IL-15, scd4l, IL-17, IL1Ra, sil-2ra, IL-1a, IL- Ghrelin, GIP, Glucagon, IL-6, Insulin, Multiplex 9, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IP-1,, MIP-1a, Leptin,, PP, PYY, TNF-a MIP-1b, RANTES, TNF-a, TNF-b, VEGF Volume Required Per Replicate 25uL 25uL 25uL 25uL Typical Dilution 1:2 None None None Manufacturer Defined Minimum Detectible Concentration 3.4 pg/ml 1.2 pg/ml 1.9 pg/ml 6. pg/ml UVM Observed Minimum Detectible Concentration pg/ml 1.2 pg/ml 1.9 pg/ml 6. pg/ml UVM Run Date No Data 4/16/213 7/31/215 1/9/215 ECS5251: ECS5251: ECS5251: ECS5251: ECS5251: ECS5251: ECS5251: ECS5251: ECS5251: ECS5251: ECS5251: ECS5251: ECS5251: ECS5251: ECS5251: ECS5251: ECS5251: ECS5251: ECS5251: ECS5251: Average #DIV/! ECS = EDTA Control Set

5 EDTA : Millipore Magnetic Adipokine Panel vs. Magnetic Cytokine Panel 6 y = x R² =.6554 EDTA : Millipore Magnetic Cytokine 6 y =.3594x R² = EDTA : Millipore Magnetic Adipokine 6 y =.7349x R² =

6 Tube Type and Freeze Thaw Comparison Sample Number Serum Citrate EDTA SCAT Average Sample Type Average Sample Type Stdev Sample Type CV% (pg/ml) Millipore Panel B Tube Type Comparison By Sample Number Sample Number Serum Citrate EDTA SCAT Sample Number Thaw 1 Thaw 2 Thaw 3 Thaw 4 Thaw 5 Thaw Serum Citrate EDTA SCAT Serum Citrate Average Freeze/Thaw Average Freeze/Thaw Stdev Freeze/Thaw CV% (pg/ml) Draw Tube Type Box Plot 17 Freeze-Thaw Box Plot Serum Citrate EDTA SCAT (pg/ml) Thaw 1 Thaw 2 Thaw 3 Thaw 4 Thaw 5 Thaw 6 Range of Standard Curve:.13-1, pg/ml Manufacturer Defined Minimum Detectable Concentration:.14 pg/ml Manufacturer Defined Intra-Assay CV% = % Manufacturer Defined Inter-Assay CV% <21% Manufacturer Recommended Sample Type: Serum or Plasma Data indicates a significant difference amongst sample types. Serum Reads the highest, at about 45% higher than the other sample types. EDTA and SCAT Appear to read nearly identical, whereas Citrate reads between EDTA/SCAT and Serum. There appears to be no effect from freeze thaw. The CV% (1.97%) of the average of all the thaws is well below the expected Intra-Assay CV range.