AMDCC Progress Report Duke/UNC/Stanford Unit. Program Director Thomas M. Coffman, MD
|
|
- Louisa Parrish
- 6 years ago
- Views:
Transcription
1 AMDCC Progress Report Duke/UNC/Stanford Unit Program Director Thomas M. Coffman, MD
2 Susceptibility Mutation Model Development + ApoE-/- +STZ Kidney Phenotype Screen Vascular & Kidney Phenotype Screen Generate ES Cell Lines From Diabetic Mice Transfer mutations to diabetic backgrounds
3 Strain Comparison Of the 5 strains tested, DBA/2 found to be most susceptible in STZ model
4
5 Albumin-to-Creatinine Ratio in Male Mice 500 *p<0.03 AC Ratio stz control 0 C57BL/6 MRL/Mp BALB/c DBA/2 Strain
6 Mesangial Volumes in Male Mice ** * *
7 Model Comparison Advantages of the Akita (Ins2 +/C96Y ) model
8 16 Week Blood Glucose Values * (*p=0.0018)
9 Differential Blood Pressure Responses * (*p= vs Akita Control)
10 Increased Proteinuria in Akita Mice * (*p=0.018 vs. STZ)
11 Mesangial Volumes in Diabetic Mice
12 C57BL/6 Ins2 +/C96Y Ins2 +/C96Y DBA/2 F 1 (C57BL / 6 x DBA/2) Ins2 +/C96Y Ins2 +/C96Y
13 Blood Glucose Levels in Mice * (*p=0.011)
14 Enhanced Proteinuria in F 1 (DBA/2 x C57BL/6)Ins2 +/C96Y Mice * (*p<0.001 vs. B6-Akita)
15 Mesangial Expansion in F 1 (DBA/2 x C57BL/6)Ins2 +/C96Y Mice * (*p<0.001 vs. B6-Akita)
16 Studies in Progress Large group of animals for long-term studies Back-crossing of Ins2 +/C96Y onto DBA/2 and other backgrounds
17 Susceptibility Mutation Model Development + ApoE-/- +STZ Kidney Phenotype Screen Vascular & Kidney Phenotype Screen Generate ES Cell Lines From Diabetic Mice Transfer mutations to diabetic backgrounds
18 Angiotensinogen Renin ACE2 Angiotensin 1-9 ACE2 Angiotensin 1-7 Angiotensin I Angiotensin II ACE Kinins Inactive Peptides AT 1 Receptor
19 Angiotensinogen Renin ACE2 Angiotensin 1-9 ACE2 Angiotensin 1-7 Angiotensin I Angiotensin II ACE Kinins Inactive Peptides AT 1 Receptor
20 Agtr1a Gene Duplication A X K 1 2 x X 3 1 K X B K X 1 K 90 kb gap TK (K)(X) Neo 2 C X K 1 2 X 3 K (X) Neo 1 2 kb 2 diagnostic PCR K X K 1 2 X 3 K X probe probe 7.5 kb 6.0 kb
21 copy 3-copy Agtr1a Genotype * 4-copy Ligand Binding Density (DPM/mm2)
22 ACR in Agtr1a 4-Copy Mice
23 Angiotensinogen Renin ACE2 Angiotensin 1-9 ACE2 Angiotensin 1-7 Angiotensin I Angiotensin II ACE Kinins Inactive Peptides AT 1 Receptor
24 ACE2 mrna Expression heart kidney sk. muscle liver lung placenta brain pancrea spleen thymus prostate testis ovary sm. intestine colon pmn s stomach thyroid spinal cord lymph node trachea adrenal gland bone marrow Donoghue et al, Circ Res 87:1, 2000
25 ACR in Ace2-Knockout Mice
26 Angiotensinogen Renin ACE2 Angiotensin 1-9 ACE2 Angiotensin 1-7 Angiotensin I Angiotensin II ACE Kinins Inactive Peptides AT 1 Receptor
27 Renin Transgenic Mice Single-copy transgene on C57BL/6 background Renin expression driven by albumin promoter
28 Blood Glucose in Renin Tg Mice
29 Blood Pressures in Renin Tg Mice
30 Albuminuria in Renin Tg Mice
31 Angiotensinogen Renin ACE2 Angiotensin 1-9 ACE2 Angiotensin 1-7 Angiotensin I Angiotensin II ACE Kinins Inactive Peptides AT 1 Receptor
32 Absence of Bradykinin B2 receptor increases diabetic albuminuria PNAS 101: , 2004
33 Bdkr2 +/+ Ins2 +/+ Bdkr2 -/- Ins2 +/+ Bdkr2 +/+ Ins2 +/C96Y Bdkr2 -/- Ins2 +/C96Y
34 Completed Screens Strain Diabetes Blood Glucose Blood AC Ratio Pathology Induction Pressure Tp -/- (C57BL/6) STZ x x x (Renal) Tp -/- (BALB/c) STZ x x x (Renal) TIMP3 -/- STZ x x x (Renal) Agtr1a dupl (2/2) STZ x x x (Renal) Bdkr2 -/- STZ x - x (Renal) Bdkr2 -/- x Ins2 Akita genetic x x x ((Renal) Nos3 -/- STZ (x) - (x) (Renal) Apoe -/- (B6) 2mo STZ x x x Renal/Vascular old Apoe -/- (B6) 6mo STZ x x x (Renal)/Vascular old Cbs -/- Apoe -/- (w/o STZ x x x (Renal)/Vascular Met) Cbs -/- Apoe -/- (w STZ x x x (Renal)/Vascular Met) F 1 (DBA/2 x genetic x x (x) (Renal) Ins2 Akita ) Ppar P435L/+ (129xB6 STZ (x) (x) (x) (Renal/Vascular) F1) 129xB6 F1 STZ (x) (x) (x) (Renal Renal)
35 Susceptibility Mutation Model Development + ApoE-/- +STZ Kidney Phenotype Screen Vascular & Kidney Phenotype Screen Generate ES Cell Lines From Diabetic Mice Transfer mutations to diabetic backgrounds
36 Diabetes exacerbates the atherosclerotic lesion in ApoE-/- mice 5 ze (x10 5 ) Lesion si ± 8 % 178 ± 8 % p= ApoE -/- Non-DM ApoE -/- DM
37 Diabetes exacerbates diabetic nephropathy in ApoE-/- mice U-Albumin Excretion (µg/30gbw/d) B6 ApoE -/- B ApoE -/- 6 mos 3 mos 6 mos # Non-DM * DM # M Non-DM D M Non-DM D Non-diabetic ApoE-/- diabetic ApoE-/-
38 % Aortic Occlusion r 2 =0.84; p = AER (ug/30g BW/d)
39
40 -CBS+/-ApoE-/- given STZ -Supplemented with L-methionine (0.5%) or L-methionine (0.5%)/Folate (200µg/L)
41 Plasma Hcy levels in apoe-/-cbs+/- Non diabetic (Non-DM) and Diabetic (DM) mice at study initiation (2 months old) and conclusion (8 months old). P<0.05, n=6.
42 Albumin Excretion Rate, AER (ug/30gbw/d) * # # # 0 Non-DM DM Non-DM DM Non-DM DM Non-DM DM B6 ApoE -/- ApoE -/- ApoE -/- CBS +/- 6 mos 3 Mos 6 Mos 6 Mos Figure 2. Albumin Excretion rate (AER) at the end of 3 or 6 months
43 35 r 2 =0.63; p=0.03 Ao Occlusion (%) AER (µg/30g/d) Proximal Aortic occlusion is related to AER in ApoE-/-CBS+/- Mice
44 New Model Development Ppar P465L/+ mouse line (Tsai et al., J Clin Invest, 2004) Increased B-cell insulin production Hypertension Abdominal fat distribution 3 UTR modification of the CTGF Gene
45
IMPC phenotyping SOPs in JMC
IMPC phenotyping SOPs in JMC Tissue Embedding and Block Banking IMPC_BLK_001 Purpose Collect and fix a standard list of tissues from the complete necropsy (see IMPC Gross Pathology & Tissue Collection
More informationMT09 - Normal Human Tissue Microarray, FDA
Reveal Biosciences offers Histochemical Staining, Immunohistochemistry (IHC), In Situ Hybridization (ISH), Whole Slide Imaging, and Quantitative Image Analysis on any TMA MT09 - Normal Human Tissue Microarray,
More informationMouse Models of Diabetic Nephropathy. Mount Sinai / Jefferson / Einstein / Minnesota Erwin Böttinger, PI Kumar Sharma, Co-PI
Mouse Models of Diabetic Nephropathy Mount Sinai / Jefferson / Einstein / Minnesota Erwin Böttinger, PI Kumar Sharma, Co-PI Group Members Mount Sinai: Phenotyping, Molecular Pathology & Validation Erwin
More informationMichigan/Chicago unit
Michigan/Chicago unit Modifications in Mouse Models to Enhance Nephropathy/Neuropathy- GLUT1 overexpression Increased oxidative stress Increased glucose metabolic flux or alteration in GLUT expression
More informationCS 6824: Tissue-Based Map of the Human Proteome
CS 6824: Tissue-Based Map of the Human Proteome T. M. Murali November 17, 2016 Human Protein Atlas Measure protein and gene expression using tissue microarrays and deep sequencing, respectively. Alternative
More informationMouse Models of Diabetic Nephropathy. Mount Sinai / Jefferson / Einstein / Minnesota Erwin Böttinger, PI Kumar Sharma, Co-PI
Mouse Models of Diabetic Nephropathy Mount Sinai / Jefferson / Einstein / Minnesota Erwin Böttinger, PI Kumar Sharma, Co-PI Group Members Mount Sinai: Phenotyping, Molecular Pathology & Validation Erwin
More informationrenoprotection therapy goals 208, 209
Subject Index Aldosterone, plasminogen activator inhibitor-1 induction 163, 164, 168 Aminopeptidases angiotensin II processing 64 66, 214 diabetic expression 214, 215 Angiotensin I intrarenal compartmentalization
More informationSupplementary Figure 1. Genotyping strategies for Mcm3 +/+, Mcm3 +/Lox and Mcm3 +/- mice and luciferase activity in Mcm3 +/Lox mice. A.
Supplementary Figure 1. Genotyping strategies for Mcm3 +/+, Mcm3 +/Lox and Mcm3 +/- mice and luciferase activity in Mcm3 +/Lox mice. A. Upper part, three-primer PCR strategy at the Mcm3 locus yielding
More informationSample validation data
Bioware and Bioware Ultra oncology cell lines Sample validation data Animal models and cell injection number A549-luc-C8 (Cat. No. 119266) Model Subcutaneous Intravenous Male nu/nu Male nu/nu (Charles
More informationMulti-normal human tissues, FDA, 96 samples, 35 organs/sites from three individuals (1.5mm)
ab178228 Multi-normal human tissues, FDA, 96 samples, 35 organs/sites from three individuals (1.5mm) Instructions for Use Designed for antibody cross reactivity analysis and IHC or ISH based protein or
More information7/4/2018. Key Objectives. A and P 2401 Lecture 2 TWO MECHANISMS USED TO MAINTAIN HOMEOSTASIS. Negative Feedback Examples. Review of Homeostasis
Key Objectives Review of Homeostasis Negative Feedback Mechanisms Positive Feedback Mechanisms Body Systems and Function A and P 2401 Lecture 2 HOMEOSTASIS TWO MECHANISMS USED TO MAINTAIN HOMEOSTASIS The
More informationDoctor of Philosophy
Regulation of Gene Expression of the 25-Hydroxyvitamin D la-hydroxylase (CYP27BI) Promoter: Study of A Transgenic Mouse Model Ivanka Hendrix School of Molecular and Biomedical Science The University of
More informationSupporting Information
Supporting Information Stegbauer et al. 10.1073/pnas.0903602106 SI Methods Analysis of Plasma Renin Activity (PRA) and ACE Activity. PRA and serum ACE activity levels were determined by RIA (RENCTK, DiaSorin;
More informationBiology Anatomy and Physiology I. Learn and Understand. What is Biology? bios = life -ology = study of
Biology 2331 Anatomy and Physiology I "If you want something you've never had, then you've got to do something you've never done." Learn and Understand A new language At this stage, science drives the
More informationDiabetic Nephropathy 2009
Diabetic Nephropathy 2009 Michael T McDermott MD Director, Endocrinology and Diabetes Practice University of Colorado Hospital Michael.mcdermott@ucdenver.edu Diabetic Nephropathy Clinical Stages Hyperfunction
More informationRadiation Dosimetry for CT Protocols
Radiation Dosimetry for CT Protocols This document contains radiation dosimetry information from CT scans and can be used by investigators to estimate the dosimetry information required by the JRSC or
More informationLaboratory Investigation 24A Chapter 24A: Human Skin
Name Class Date Station # Laboratory Investigation 24A Chapter 24A: Human Skin Human Anatomy & Physiology: Integumentary System You may refer to pages 415-421 in your textbook for a general discussion
More informationEndocrine Glands and Hormones "What are endocrine glands and what do they make?
Endocrine Glands and Hormones "What are endocrine glands and what do they make? Model 1:Development of glands. Exocrine Gland Endocrine Gland Critical thinking questions 1. Based on the model, which of
More informationARGININE VASOPRESSIN (AVP)
ARGININE VASOPRESSIN (AVP) AFFECTS BLOOD PRESSURE AND RENAL WATER REABSORPTION WHAT ELSE DOES IT DO? Michael F. Michelis, M.D., F.A.C.P., F.A.S.N. Director, Division of Nephrology Lenox Hill Hospital,
More informationINTRODUCTION TO ANIMALS
AP BIOLOGY ANIMALS ACTIVITY #1 NAME DATE HOUR INTRODUCTION TO ANIMALS LEVELS OF ORGANIZATION Animals Activity #1 page 1 HOMEOSTASIS: DEFINITION IMPORTANCE MECHANISMS FOR MAINTAINING HOMEOSTASIS: Animals
More informationOVARIES URETER FALLOPIAN TUBES BLADDER UROGENITAL OPENINGS (BOTH SEXES) PENIS VAGINA UTERUS
URETER OVARIES FALLOPIAN TUBES BLADDER UROGENITAL OPENINGS (BOTH SEXES) PENIS VAGINA UTERUS REPRODUCTIVE PRODUCE FEMALE HORMONES EXCRETORY FROM KIDNEY TO BLADDER EXCRETORY STORES URINE REPRODUCTIVE TRANSPORTS
More informationDate Lab Pd. Lecture Notes (57)
Name SECTION OBJECTIVES Describe the locations of the major body cavities List the organs located in each major body cavity Name the membranes associated with the thoracic and abdominopelvic cavities Name
More informationMouse Models for Studying Human Islet Transplantation
Mouse Models for Studying Human Islet Transplantation Ronald G. Gill, Joshua Beilke,, Nathan Kuhl,, Michelle Kerklo, and Mark M. Nicolls Barbara Davis Center for Childhood Diabetes University of Colorado
More informationSHN-1 Human Digestive Panel Test results
SHN-1 Human Digestive Panel Test results HN-30 tongue HN-24 salivary gland HN-12 larynx HN-28 esophagus HN-29 stomach HN-20 pancreas HN-13 liver HN-14 gall bladder HN-27-1 duodenum HN-27-2 ileum HN-27-3
More informationBIO 137 Human Anatomy & Physiology I. Laboratory Manual. Laboratory #1: Measurements, Body Organization and Anatomical Systems
BIO 137 Human Anatomy & Physiology I Laboratory Manual Laboratory #1: Measurements, Body Organization and Anatomical Systems Lab Exercise 1 Measurements Body Organization Body Systems What you need to
More informationFigure S1. Reduction in glomerular mir-146a levels correlate with progression to higher albuminuria in diabetic patients.
Supplementary Materials Supplementary Figures Figure S1. Reduction in glomerular mir-146a levels correlate with progression to higher albuminuria in diabetic patients. Figure S2. Expression level of podocyte
More informationCrosswalk File of ICD9 Diagnosis Codes to Risk Group Assignment 1-Apr-15
1 1500 MALIGNANT NEOPLASM OF CERVICAL ESOPHAGUS 1 1501 MALIGNANT NEOPLASM OF THORACIC ESOPHAGUS 1 1502 MALIGNANT NEOPLASM OF ABDOMINAL ESOPHAGUS 1 1503 MALIGNANT NEOPLASM OF UPPER THIRD OF ESOPHAGUS 1
More informationFAX, A FEMALE ADULT VOXEL PHANTOM FOR RADIATION PROTECTION DOSIMETRY
EFFECTIVE DOSE RATIOS FOR THE TOMOGRAPHIC MAX AND FAX PHANTOMS ******************************************************** EFFECTIVE DOSE RATIOS FOR TOMOGRAPHIC AND STYLIZED MODELS FROM > EXTERNAL EXPOSURE
More informationReview of 10 major human body systems using a puzzle technique. Systems Shuffle. By: Heidi Hisrich of The Dork Side
Review of 10 major human body systems using a puzzle technique Systems Shuffle By: Heidi Hisrich of The Dork Side Teaching students about the different human body systems is one of my favorite things to
More informationAdrenal Glands. Adrenal Glands. Adrenal Glands. Adrenal Glands. Adrenal Glands 4/12/2016. Controlled by both nerves and hormones.
Glands http://www.hawaiilife.com/articles/2012/03/good-news-vacation-rental-owners/ 70 Figure 10.14a gland Glands cortex Mineralocorticoids Gonadocorticoids Glucocorticoids medulla Epinephrine Norepinephrine
More informationThe Endocrine System. I. Overview of the Endocrine System. II. Three Families of Hormones. III. Hormone Receptors. IV. Classes of Hormone Receptor
The Endocrine System I. Overview of the Endocrine System A. Regulates long term metabolic processes B. Releases hormones from endocrine cells 1. Hormones are chemicals 2. Alter metabolism of cells 3. Release
More informationCancer in Estonia 2014
Cancer in Estonia 2014 Estonian Cancer Registry (ECR) is a population-based registry that collects data on all cancer cases in Estonia. More information about ECR is available at the webpage of National
More informationINTEREST GRABBER NOTEBOOK #1
INTEREST GRABBER NOTEBOOK #1 AN IMPORTANT PROCESS While walking along a dusty path, you begin to cough. As you continue your walk, a small insect comes flying toward you. You blink and then duck so that
More informationWarm Up Where in a flower would you find xylem and phloem? 2. Where in a flower would you find palisade cells?
Body Systems Warm Up 4-4-16 1. Where in a flower would you find xylem and phloem? 2. Where in a flower would you find palisade cells? 3. Where in a flower would you find root hair cells? 4. What organelle
More informationBody Systems Notes. Nervous, Integumentary, Immune/Lymphatic, Circulatory, Skeletal, Respiratory, Digestive, Excretory, Endocrine, Reproductive
Body Systems Notes Nervous, Integumentary, Immune/Lymphatic, Circulatory, Skeletal, Respiratory, Digestive, Excretory, Endocrine, Reproductive Homeostasis: maintaining a balance. Examples: temperature,
More informationCells Tissues Organs Organ Systems Organism. Cells: the smallest unit of life.
Cells Tissues Organs Organ Systems Organism Cells: the smallest unit of life. The Circulatory Systems brings oxygen, nutrients and hormones to cells; fights infections; removes cell wastes; regulates
More informationOverview of Anatomy & Physiology
Overview of Anatomy & Physiology Anatomy the study of the structure of body parts and their relationships to one another Gross or macroscopic Microscopic Developmental Physiology the study of the function
More informationHuman Body Systems Pre-AP Project
Human Body Systems Pre-AP Project Congratulations! You have surpassed enough biology curriculum this school year that you are ready to become the teacher for a day! You and 2-3 other classmates will research
More informationTime Allotment: 1½ hours (rat dissection 1 hour; human torso model ½ hour).
Human Anatomy Laboratory Manual with Cat Dissections 8th Edition Marieb SOLUTIONS MANUAL Full download at: https://testbankreal.com/download/human-anatomy-laboratory-manual-with-catdissections-8th-edition-marieb-solutions-manual/
More informationDr. Hayam Gad Associate Professor of Physiology College of Medicine King Saud University
Dr. Hayam Gad Associate Professor of Physiology College of Medicine King Saud University INTRODUCTION Diabetic nephropathy (DN) DN is defined as the appearance of persistent clinical albuminuria in an
More informationAnatomy. Contents Brain (Questions)
Anatomy 12 Contents 12.1 Brain (Questions).................................................... 683 12.2 Head and Neck (Questions)............................................. 685 12.3 Thorax (Questions)...................................................
More informationNervous System. Skeletal System. Muscular System. Reproductive System. Circulatory System. Endocrine System. Respiratory System. Integumentary System
The Human Body Skeletal System Muscular System Circulatory System Respiratory System Digestive System Nervous System Reproductive System Endocrine System Integumentary System Excretory System Lymphatic/Immune
More informationstudy of body structure & Physiology study of body function
study of body structure & Physiology study of body function Medical Words Made up of Latin, Greek, French, German, Dutch, Scandinavian, Italian, English, etc.. Common Medical Roots, Prefixes & Suffixes
More informationFeinberg School of Medicine Chicago IL Division of Nephrology and Hypertension
Feinberg School of Medicine Chicago IL Division of Nephrology and Hypertension Daniel Batlle MD Earle, del Greco Levin Professor of Medicine Division of Nephrology and Hypertension Northwestern University
More informationEXERCISE Organ Systems Overview
Solutions Manual Human Anatomy & Physiology Laboratory Manual, Fetal Pig Version 12th Edition Marieb Smith Instructor guide for for Human Anatomy & Physiology Laboratory Manual, Fetal Pig Version 12th
More informationHeal a th t y h y B o B dy d Systems
Chapter 16 Healthy Body Systems The Cell The smallest unit of structure in a living organism Robert Hooke Identified & coined the term Cell when he saw cork under the microscope He used a compound microscope
More informationCase Study in Chronic Renal Failure
Case Study in Chronic Renal Failure Development of Knowledge Base: There were over 14,500 articles dealing with chronic renal failure entered into PubMed during 2000 2004. A current concept in this array
More informationHormones. Introduction to Endocrine Disorders. Hormone actions. Modulation of hormone levels. Modulation of hormone levels
Introduction to Endocrine Disorders Hormones Self-regulating system (homeostasis) Affect: Growth Metabolism Reproduction Fluid and electrolyte balance Hormone actions Endocrine gland Hormone synthesis
More informationA Central Role of MG53 in Metabolic Syndrome. and Type-2 Diabetes
A Central Role of MG53 in Metabolic Syndrome and Type-2 Diabetes Yan Zhang, Chunmei Cao, Rui-Ping Xiao Institute of Molecular Medicine (IMM) Peking University, Beijing, China Accelerated Aging in China
More informationHuman Anatomy and Physiology (ANAT 5) Mrs. Fulton Phone: ext. 6049
Human Anatomy and Physiology (ANAT 5) Mrs. Fulton Phone: 645-1300 ext. 6049 Please, read your green sheets. 1 Lecture #1 A. Definitions: anatomy, physiology B. Requirements of an Organism C. Homeostasis
More information>4000 mg/dl (=20000/(500/100)) >615 mmol/l (=20000/(65*0.5))
Supplemental Table 1 Thresholds to define outliers of lab values in screening test for proteinuria Type of screening test Unit Threshold for Outliers References 24 hour urine albumin mg/d >20000 experienced
More informationTransgenic Mice and Genetargeting
Transgenic Mice and Genetargeting mice In Biomedical Science Techniques of transgenic and gene-targeting mice are indispensable for analyses of in vivo functions of particular genes and roles of their
More informationQuality ID #119 (NQF 0062): Diabetes: Medical Attention for Nephropathy National Quality Strategy Domain: Effective Clinical Care
Quality ID #119 (NQF 0062): Diabetes: Medical Attention for Nephropathy National Quality Strategy Domain: Effective Clinical Care 2018 OPTIONS F INDIVIDUAL MEASURES: REGISTRY ONLY MEASURE TYPE: Process
More informationNatural Hormones Replacement An Evidence and Practice Based Approach
Natural Hormones Replacement An Evidence and Practice Based Approach Andres Ruiz, PharmD, MSc, FACA President/Partner Stonegate Pharmacy PRESENTED BY THE AMERICAN COLLEGE OF APOTHECARIES 2830 SUMMER OAKS
More informationSpecies Tumor Type Comment for in vivo work Lead Time for in vivo studies [weeks] MB-49-luc-2 Mouse urinary bladder carcinoma C57BL/6 2
America, Hershey, PA Australia, Melbourne, VIC Europe, Munich info@vivopharm.com www.vivopharm.com Tissue Bladder Species Tumor Type Comment for in vivo work Lead Time for in vivo studies [weeks] MB-49-luc-
More informationCardiovascular diseases identification of genomic markers Potential interest, limitations
Cardiovascular diseases identification of genomic markers Potential interest, limitations Degenerative cardiovascular diseases Complexity of anatomical and clinical phenotypes (arterial remodeling, obstruction,
More informationHuman Anatomy & Physiology
Human Anatomy & Physiology Hey I thought those were the same thing! Nope they ain t Anatomy-Where everything is and to what it is connected. Physiology-How all that stuff works to keep you alive! Morphology-How
More informationEndocrine System Hormones. AP Biology
Endocrine System Hormones 2007-2008 Regulation Why are hormones needed? u chemical messages from one body part to another u communication needed to coordinate whole body u daily homeostasis & regulation
More informationMEDICAL POLICY Gene Expression Profiling for Cancers of Unknown Primary Site
POLICY: PG0364 ORIGINAL EFFECTIVE: 04/22/16 LAST REVIEW: 07/26/18 MEDICAL POLICY Gene Expression Profiling for Cancers of Unknown Primary Site GUIDELINES This policy does not certify benefits or authorization
More informationLN04 - Lymphoma Tissue Microarray
Reveal Biosciences offers Histochemical Staining, Immunohistochemistry (IHC), In Situ Hybridization (ISH), Whole Slide Imaging, and Quantitative Image Analysis on any TMA LN04 - Lymphoma Tissue Microarray
More informationDescriptive Histology
Atlas of Descriptive Histology Michael H. Ross University of Florida College of Medicine Gainesville, Florida Wojciech Pawlina Mayo Medical School College of Medicine, Mayo Clinic Rochester, Minnesota
More informationDissection Lab Manuals: Required Content
Dissection Lab Manuals: Required Content 1. Introduction a. Basic terminology (directions) b. External features of the cat c. Adaptations to predatory niche d. How to skin a cat e. How to make the incisions
More informationFebruary [KU 1010] Sub. Code: 4701
February 2009 [KU 1010] Sub. Code: 4701 B.Sc (Nursing ) DEGREE EXAMINATION Maximum : 75 marks SECTION A (ANATOMY) 1. Describe the uterus under the following heads: a) Situation b) Normal position c) Parts
More information2005 RECOMMENDATIONS OF ICRP
IRPA 11 11 th International Congress of the International Radiation Protection Association 23 28 May 2004, Madrid, Spain 2005 RECOMMENDATIONS OF ICRP ROGER H CLARKE CHAIRMAN FEATURES OF RECOMMENDATIONS
More informationCEA (CARCINOEMBRYONIC ANTIGEN)
(CARCINOEMBRYONIC ANTIGEN) 428 C15.3 Malignant neoplasm of upper third of esophagus C15.4 Malignant neoplasm of middle third of esophagus C15.5 Malignant neoplasm of lower third of esophagus C15.8 Malignant
More informationA Time- and Resource-Efficient Method for Annually Auditing All Reporting Hospitals in Your State: the Inpatient & Outpatient Hospital Discharge Files
A Time- and Resource-Efficient Method for Annually Auditing All Reporting Hospitals in Your State: the Inpatient & Outpatient Hospital Discharge Files By Dr. Martin A. Whiteside Director, Office of Cancer
More informationSalt Sensitivity: Mechanisms, Diagnosis, and Clinical Relevance
Salt Sensitivity: Mechanisms, Diagnosis, and Clinical Relevance Matthew R. Weir, MD Professor and Director Division of Nephrology University of Maryland School of Medicine Overview Introduction Mechanisms
More informationChapter 21. Human Endocrine System
Chapter 21 Human Endocrine System Endocrine System Consist of endocrine glands that secrete hormones which regulate a wide range of activities in humans Hormones- are secreted by glands and diffuse directly
More informationKinex Reverse Lysate Microarray KRLM-1.0. Case Study
Kinex Reverse Lysate Microarray KRLM-1. Case Study Probing with an Anti-phospho-ERK1/2 Antibody The Kinex Reverse Lysate Microarray (KRLM) is a powerful tool for rapid characterization of a protein for
More informationCat Dissection. Muscular Labs
Cat Dissection Muscular Labs Tibialis anterior External oblique Pectroalis minor Gastrocnemius Sartorius Pectoralis major Gastrocnemius Semitendinosis Sartorius External oblique Trapezius Latissimus dorsi
More informationStages of Chronic Kidney Disease (CKD)
Early Treatment is the Key Stages of Chronic Kidney Disease (CKD) Stage Description GFR (ml/min/1.73 m 2 ) >90 1 Kidney damage with normal or GFR 2 Mild decrease in GFR 60-89 3 Moderate decrease in GFR
More informationDiabetes and Kidney Disease. Kris Bentley Renal Nurse practitioner 2018
Diabetes and Kidney Disease Kris Bentley Renal Nurse practitioner 2018 Aims Develop an understanding of Chronic Kidney Disease Understand how diabetes impacts on your kidneys Be able to recognise the risk
More informationCancer Program Report 2014
Cancer Program Report 2014 Queen of the Valley Hospital St Joseph Health Queen of the Valley Hospital - 2014 Site Table Site Total Class Sex Group Cases Analytic NonAn M F 0 I II ALL SITES 661 494 167
More informationEndocrine System Hormones (Ch. 45)
Endocrine System Hormones (Ch. 45) Regulation Why are hormones needed? chemical messages from one body part to another communication needed to coordinate whole body daily homeostasis & regulation of large
More informationThe CARI Guidelines Caring for Australasians with Renal Impairment. Protein Restriction to prevent the progression of diabetic nephropathy GUIDELINES
Protein Restriction to prevent the progression of diabetic nephropathy Date written: September 2004 Final submission: September 2005 Author: Kathy Nicholls GUIDELINES a. A small volume of evidence suggests
More informationIntro to Animal Structure & Function
Intro to Animal Structure & Function Convergence on streamlined swimming shape 1 Epithelial tissue 2 Exocrine glands Cilia Unicellular glands (goblet cells) Basement membrane (a) Goblet cells Skin (b)
More informationAnalysis of AVP functions via V1a and V1b receptors with knockout mice. Akito Tanoue
Analysis of AVP functions via V1a and V1b receptors with knockout mice Akito Tanoue Department of Pharmacology, National Research Institute for Child Health and Development Arginine-Vasopressin (AVP) is
More informationChapter 20 UNIFYING CONCEPTS OF ANIMAL STRUCTURE AND FUNCTION
Chapter 20 UNIFYING CONCEPTS OF ANIMAL STRUCTURE AND FUNCTION I. Life is based on many structural levels Levels of animal structure: Atoms and molecules Cells Tissues Organs Organ systems Organism: May
More informationNephropathy in Type 1 Diabetes: Can One Identity the Patients at Risk?
Nephropathy in Type 1 Diabetes: Can One Identity the Patients at Risk? Pierre Lefèbvre University of Liège, Belgium Cuba, November 2007 Nephropathy in Type 1 Diabetes It has been known for years that the
More information1. How did occlusion of Mr. Hanna's left renal artery lead to an increase in plasma renin activity?
76 I'HYSIOLOGY CASES AND PROBLEMS Case 14 Renovascular Hypertension: The Renin-Angiotensin-Aldosterone System Stewart Hanna is a 58-year-old partner in a real estate firm. Over the years, the pressures
More informationDiabetic nephropathy (DN) is the major single cause of
JASN Express. Published on November 24, 2004 as doi: 10.1681/ASN.2004080648 Mouse Models of Diabetic Nephropathy Matthew D. Breyer,* Erwin Böttinger, Frank C. Brosius, III, Thomas M. Coffman, Raymond C.
More informationCHAPTER 1 THE HUMAN BODY
HPTER 1 THE HUMN OY TRUE/FLSE 1. midsagittal plane vertically divides the body through the midline into two equal left and right portions or halves. NS: T NOT: midsagittal plane vertically divides the
More information5. March 23, Seminar: Relationship between structure and function of the liver. Practical class: Gastro-intestinal system, part 3.
Katedra i Zakład Histologii i Embriologii Centrum Biostruktury Warszawski Uniwersytet Medyczny 2014/2015 SECOND SEMESTER HISTOLOGY & EMBRYOLOGY 6 years MD PROGRAM SEMINARS & PRACTICAL CLASSES SPRING SEMESTER
More informationOFCCR CLINICAL DIAGNOSIS AND TREATMENT FORM
OFCCR CLINICAL DIAGNOSIS AND TREATMENT FORM Name: _, OFCCR # _ OCGN # _ OCR Group # _ HIN# Sex: MALE FEMALE UNKNOWN Date of Birth: DD MMM YYYY BASELINE DIAGNOSIS & TREATMENT 1. Place of Diagnosis: Name
More informationDistribution of Trichinella spiralis Larvae in Tissues of Swine
Proceedings of the Iowa Academy of Science Volume 68 Annual Issue Article 78 1961 Distribution of Trichinella spiralis Larvae in Tissues of Swine W. J. Zimmermann Iowa State University L. H. Schwarte Copyright
More informationChapter 13 worksheet
Name: Chapter 13 worksheet The Endocrine System Please label the: hypothalamus pineal gland pituitary gland thyroid gland parathyroid gland thymus heart stomach liver adrenal glands kidneys pancreas small
More informationSonography. 1. Introduction. 2. Documentation of Compliance. 3. Didactic Competency Requirements. 4. Clinical Competency Requirements
PRIMARY CERTIFICATION Sonography 1. Introduction Candidates for certification and registration are required to meet the Professional Education Requirements specified in the ARRT Rules and Regulations.
More informationChapter 9 The Endocrine System and Hormone Activity
Chapter 9 The Endocrine System and Hormone Activity Overview Coordinates and directs the activity of cells. Interacts with the nervous system Uses chemical messengers called hormones released by organs
More informationRefer to the figure below, a diagram of a renal tubule, to answer the following questions.
1. The digestion and utilization of which nutrient creates the greatest need for osmoregulation by the kidneys? a. protein b. starch c. fat d. oil e. cellulose 2. Which of the following is true of urea?
More informationArtery 1 Head and Thoracic Arteries. Arrange the parts in the order blood flows through them.
Artery 1 Head and Thoracic Arteries 1. Given the following parts of the aorta: 1. abdominal aorta 2. aortic arch 3. ascending aorta 4. thoracic aorta Arrange the parts in the order blood flows through
More informationHot Topics in Diabetic Kidney Disease a primary care perspective
Hot Topics in Diabetic Kidney Disease a primary care perspective DR SARAH DAVIES GP PARTNER WITH SPECIAL INTEREST IN DIABETES, CARDIFF DUK CLINICAL CHAMPION NB MEDICAL HOT TOPICS PRESENTER AND DIABETES
More informationAMDCC Neuropathy Phenotyping Core. Eva Feldman MD, PhD JDRF Center University of Michigan
AMDCC Neuropathy Phenotyping Core Eva Feldman MD, PhD JDRF Center University of Michigan 1 Outline Define neuropathy and illustrate phenotyping Summarize current phenotyping efforts Collaborations 2 AMDCC
More informationMales- Western Diet WT KO Age (wks) Females- Western Diet WT KO Age (wks)
Relative Arv1 mrna Adrenal 33.48 +/- 6.2 Skeletal Muscle 22.4 +/- 4.93 Liver 6.41 +/- 1.48 Heart 5.1 +/- 2.3 Brain 4.98 +/- 2.11 Ovary 4.68 +/- 2.21 Kidney 3.98 +/-.39 Lung 2.15 +/-.6 Inguinal Subcutaneous
More informationSentelligent Medical Intuitive Body Scan
Sentelligent Medical Intuitive Body Scan 1 1) Ask for presenting symptoms. Get clear channel and set sacred space. 2) Ask if any resistance or interference. 3) Ask Source to provide information only on
More informationAMDCC Neuropathy Core March Eva L. Feldman, M.D., Ph.D Department of Neurology JDRF Center University of Michigan
AMDCC Neuropathy Core March 6 Eva L. Feldman, M.D., Ph.D Department of Neurology JDRF Center University of Michigan AMDCC Definition of Neuropathy Evidence of clinical loss of sensory function Tail flick
More informationThe role of apolipoprotein D in lipid metabolism and metabolic syndrome
UQÀM: Department of Biological Sciences The role of apolipoprotein D in lipid metabolism and metabolic syndrome Catherine Mounier PhD Apolipoprotein D Apolipoprotéine D (apod) Secreted protein Associated
More information2019 COLLECTION TYPE: MIPS CLINICAL QUALITY MEASURES (CQMS) MEASURE TYPE: Process
Quality ID #119 (NQF 0062): Diabetes: Medical Attention for Nephropathy National Quality Strategy Domain: Effective Clinical Care Meaningful Measure Area: Management of Chronic Conditions 2019 COLLECTION
More informationThe Diabetes Kidney Disease Connection Missouri Foundation for Health February 26, 2009
The Diabetes Kidney Disease Connection Missouri Foundation for Health February 26, 2009 Teresa Northcutt, RN BSN Primaris Program Manager, Prevention - CKD MO-09-01-CKD This material was prepared by Primaris,
More informationEndocrine System WHO IS IN CONTROL?
Endocrine System WHO IS IN CONTROL? Objectives Explain how the endocrine and nervous system work together to regulate bodily functions Describe the basic anatomy of the endocrine system Describe the functions
More information