Product Datasheet. beta Amyloid Antibody (MOAB-2) NBP Unit Size: 0.1 ml

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1 Product Datasheet beta Amyloid Antibody (MOAB-2) NBP Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Publications: 11 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 12/3/2017 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NBP beta Amyloid Antibody (MOAB-2) Product Information Unit Size Concentration Storage Clonality Clone Preservative Isotype Purity Buffer Product Description Host 0.1 ml 1.0 mg/ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Monoclonal MOAB % Sodium Azide IgG2b Protein G purified PBS Mouse Gene ID 351 Gene Symbol Species Reactivity Notes Specificity/Sensitivity APP Human, Mouse, Rat Human, mouse and rat. Unaggregated, oligomeric and fibrillar forms of beta Amyloid 42 and unaggregated beta Amyloid 40. Does not detect APP. Immunogen Recombinant human beta Amyloid 42 Notes Product Application Details Applications 5xFAD mice = transgenic mice expressing 5 familial Alzheimer's disease mutation. See Oakley H et al, J Neurosci Oct 4;26(40): (PMID: ) for more information on 5xFAD mice. Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunohistochemistry Free-Floating Recommended Dilutions Western Blot 1:1000-1:5000, ELISA 1:100-1:1000, Immunohistochemistry 1:40-1:400, Immunocytochemistry/Immunofluorescence 1:200-1:500, Immunoprecipitation 1:200-1:1000, Immunohistochemistry-Paraffin 1:40-1:400, Immunohistochemistry Free-Floating Application Notes Page 1 of 5 v.20.1 Updated 12/3/2017 This beta Amyloid Antibody (MOAB-2) is useful for Immunocytochemistry/Immunofluorescence, ELISA, Immunoprecipitation, Western Blot, and Immunohistochemistry on paraffin-embedded sections. In Western blot, a band can be seen at ~4 kda, representing the beta Amyloid monomer. Larger bands may also be seen representing the unaggregated, oligomeric, and fibrillar forms of beta Amyloid. For higher beta Amyloid yield in WB, please follow the extraction protocol described in Youmans et al, J Neurosci Methods March 15; 196(1): (PMID: ). Use in Immunohistochemistry-Free floating reported in scientific literature (PMID )

3 Images Immunohistochemistry: beta Amyloid Antibody (MOAB-2) [NBP ] - IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse brain. Page 2 of 5 v.20.1 Updated 12/3/2017 Immunohistochemistry-Paraffin: beta Amyloid Antibody (MOAB-2) [NBP ] - IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:200 dilution of anti-beta Amyloid antibody (clone MOAB-2). The staining was developed with HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This beta Amyloid antibody specifically stained the cells with Abeta 42/ Abeta aggregates while the normal cells were negative for abeta peptide. Western Blot: beta Amyloid Antibody (MOAB-2) [NBP ] - Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid (MOAB-2, Lanes 3 and 4). Beta Amyloid (MOAB-2) does not detect APP (from PMID: ). Western Blot: beta Amyloid Antibody (MOAB-2) [NBP ] - Analysis of beta Amyloid (MOAB-2) antibody in 1) 100 pmole beta Amyloid 42, 2) 5xFAD mouse brain homogenate Repetition 1 and 3) 5xFAD mouse brain homogenate Repetition 2.

4 Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody (MOAB-2) [NBP ] - Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um (from PMID: ). Page 3 of 5 v.20.1 Updated 12/3/2017 Immunohistochemistry-Paraffin: beta Amyloid Antibody (MOAB-2) [NBP ] - IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:40 dilution of anti-beta Amyloid antibody (clone MOAB-2). The staining was developed with HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This beta Amyloid antibody specifically stained the cells with Abeta 42/ Abeta aggregates while the normal cells were negative for abeta peptide.

5 Publications Page 4 of 5 v.20.1 Updated 12/3/2017 Paspalas CD, Carlyle BC, Leslie S et al. The aged rhesus macaque manifests Braak stage III/IV Alzheimer's-like pathology. Alzheimers Dement 2017 Dec 11 [PMID: ] (ICC/IF, Monkey) Lee DY, Hwang CJ, Choi JY et al. KRICT-9 inhibits neuroinflammation, amyloidogenesis and memory loss in Alzheimer's disease models. Oncotarget Sep 15 [PMID: ] (ICC/IF, Mouse) Stuart KE, King AE, Fernandez-Martos CM et al. Environmental novelty exacerbates stress hormones and AB pathology in an Alzheimer's model. Sci Rep Jun 05 [PMID: ] (ICC/IF, Mouse) Balczon R, Morrow KA, Zhou C et al. Pseudomonas aeruginosa infection liberates transmissible, cytotoxic prion amyloids. FASEB J. Mar :00AM [PMID: ] Stuart KE, King AE, Fernandez-Martos CM et al. Mid-life environmental enrichment increases synaptic density in CA1 in a mouse model of Ab-associated pathology and positively influences synaptic and cognitive health in healthy ageing. J. Comp. Neurol. Dec :00AM [PMID: ] Yu YW, Hsieh TH, Chen KY et al. Glucose-dependent insulinotropic polypeptide ameliorates mild traumatic brain injury-induced cognitive and sensorimotor deficits and neuroinflammation in rats J. Neurotrauma Mar :00AM [PMID: ] (WB, Rat) Lee CL, Lin PY, Hsu YW, Pan TM. Monascus-fermented monascin and ankaflavin improve the memory and learning ability in amyloid beta-protein intracerebroventricular-infused rat via the suppression of Alzheimer's disease risk factors. Journal of Functional Foods Oct 01 (IHC-Fr, Rat) Details: beta Amyloid antibody used for immunohistochemical staining of rat brain frozen tissue at a dilution of 1:100 (Fig. 7B). Collins JM, King AE, Woodhouse A et al. The effect of focal brain injury on beta-amyloid plaque deposition, inflammation and synapses in the APP/PS1 mouse model of Alzheimer's disease Exp. Neurol Mar 04 [PMID: ] (IHC-FrFl, Mouse) Details: beta Amyloid antibody (clone MOAB-2) used for IHC immunostaining on free floating brain sections from experiments involving focal brain injury in 3 or 9 months old male APPSWE/PSENdE9 (APP/PS1) transgenic mice (a murine model of Alzheimer's disease, Jankowsky et al Biomol Eng. 17: ) and C57BL/6 wild-type mice. The assay involved 4% paraformaldehyde-pbs fixation, 8 minutes of antigen retrieval with 88% formic acid at RT, permeablization 3X10 minutes each using 0.25% Triton-X-100-PBS, 15 minutes blocking with Dako serum-free protein block, primary used at 1:2000 dilution with ON 4C incubation, detection using Alexa-fluorophore conjugated secondary antibodies (see full text for detailed protocol and staining images). Kim JA, Yun HM, Jin P et al. Inhibitory effect of a 2,4-bis(4-hydroxyphenyl)but-2-enal diacetate on neuro-inflammatory reactions via inhibition of STAT1 and STAT3 activation in cultured astrocytes and microglial BV-2 cells. Neuropharmacology 2013 Jul 23 [PMID: ] (ICC/IF, Rat) Youmans KL, Tai LM, Nwabuisi-Heath E et al. APOE4-specific Changes in A? Accumulation in a New Transgenic Mouse Model of Alzheimer Disease J Biol Chem 2012 Dec 7 [PMID: ] (ELISA, IHC, Human) Tai, LM, Youmans, K et al. Intraneuronal Abeta detection in 5xFAD mice by a new Abeta-specific antibody Molecular Neurodegeneration 2012 [PMID: ] (ICC/IF, WB, IP, DB, Mouse)

6 Novus Biologicals USA 8100 Southpark Way, A-8 Littleton, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NBP HAF007 NB720-B NBP Goat anti-mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] Mouse IgG2b Isotype Control (MPC-11) NBP AF488 beta Amyloid Antibody (MOAB-2) [Alexa Fluor 488] Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product:

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