RADIOIMMUNOASSAY FOR GASTRIC INHIBITORY POLYPEPTIDE
|
|
- Margaret Walsh
- 6 years ago
- Views:
Transcription
1 GASTROENTEROLOGY 66: , 1974 Cpyright 1974 by The Williams & Wilkins C, Vl. 66. N.3 Printed in U.S.A. RADIOIMMUNOASSAY FOR GASTRIC INIBITORY POLYPEPTIDE MARYANNE KUZIO, JILL R. DRYBURG, KATLEEN M. MALLOY, AND JON C. BROWN, P.D. Departments f Physilgy and Surgery, University f British Clumbia, Vancuver. British Clumbia. Canada A specific radiimmunassay fr gastric inhibitry plypeptide has been develped using guinea pig antisera raised t prcine gastric inhibitry plypeptide. ighly purified 125I-prcine gastric inhibitry plypeptide, further purified n Sephadex G-15 after labeling, was emplyed as a tracer. The sensitivity range is 25 t 25 pg. N crss reactivity culd be demnstrated with gastrin, glucagn, secretin, chlecystkinin-pancrezymin, mtilin, and vasactive intestinal peptide. Reprducibility f determinatins in different assays was demnstrated. The mean fasting serum cncentratin was 237 ± 14 pg per ml in 48 nrmal subjects. Serum cncentratins increased t abve 1,2 pg after a meal. Gastric inhibitry plypeptide (GIP) btained frm prcine dudenal mucsa extracts has been islated and purified, and the amin acid cmpsitin and sequence were determined.1-5 Physilgical studies have revealed that the plypeptide will inhibit acid secretin in the dg, stimulated by pentagastrin, insulin hypglycemia, and histamine. The ability f the plypeptide t inhibit histamine- and in- Received March 19, Accepted Octber 9, Address requests fr reprints t: Dr. Jhn C. Brwn, Department f Physilgy, University f Brit ish Clumbia, Vancuver 8, B. C., Canada. This wrk was supprted by grant MT 1972 frm the Medical Research Cuncil f Canada, and by a grant frm the Banting Research Fundatin t J. C. Brwn. The authrs wish t acknwledge the cntinuing supprt f Prfessr V. Mutt and Emeritus Prfessr E. Jrpes f the Karlinska Institutet, Stckhlm, Sweden, wh dnated pure natural secretin and chlecystkinin pancrezymin. rmnes were als supplied by Dr. E. Wunsch, Max Planck-Institut fur Eiweiss und Lederfrschung, Munich (synthetic glucagn, and synthetic human gastrin, 15 leucine substituted gastrin.) Drs. S. Blm and J. Plak supplied a small sample f vasactive intestinal peptide btained frm Dr. S. Said. 357 sulin hypglycemia-stimulated acid secretin shwed it t pssess gastric inhibitry prperties different frm secretin and chlecystkinin-pancrezymin (CCK PZ).6 Prerequisites fr the establishment f GIP as a hrmne have t include the demnstratin f the plypeptide in bld and tissues and a release frm tissues by physilgical mechanisms. In general, the gastrintestinal plypeptides have a multiplicity f actins, and quite ften different plypeptides will prduce similar respnses. Classical physilgical experiments using secretaggues in the dudenum, and measuring a physilgical respnse, have prved t be unsuitable t demnstrate the mechanisms prducing the release f GIP frm tissues int the bld. This study describes the prductin by immunizatin in guinea pigs f antibdies t prcine GIP and the develpment f a radiimmunassay f sensitivity suitable fr the detectin f GIP in sera f man. These antisera prduced by immunizatin f guinea pigs with relatively small amunts f prcine GIP have n measurable crss reactivity with natural secretin, synthetic glucagn, synthetic human gas-
2 358 KUZIO ETAL. Vl. 66, N.3 trin (15-Leu gastrin), pure prcine CCK-PZ, pure prcine mtilin, and pure prcine vasactive intestinal peptide. Materials and Methds Prductin f antisera. Tw preparatins f GIP have been used fr immunizatin. They were the EG stage lip and EG stage III which was further purified by rechrmatgraphy n Sephadex G 25 fine with.2 M acetic acid as eluting buffer. The preparatins were injected subcutaneusly in at least tw sites n the lwer abdmen;.4.umle f plypeptide was used per immunizatin. Initial immunizatins were perfrmed using an emulsin frmed with Freund's adjuvant r an emulsin frmed with Freund's adjuvant after cnjugatin f the ply peptide t bvine serum albumin. Cnjugates were prepared using the carbdiimide methd. 7 The degree f cnjugatin was assessed using high vltage electrphresis at p 6.5, after which the presence f free GIP culd nt be bserved. Bld was btained by cardiac puncture 8 t 12 days after immunizatin. Three f 6 guinea pigs had antibdies detectable at an tiserum dilutins f abut 1: 3 after eight t 11 immunizing dses f uncnjugated material. These animals were given ne immunizatin with cnjugated GIP, after which all demnstrated titers in excess f 1: 5,. After a secnd immunizatin with cnjugated material, 2 f these guinea pigs had titers f 1: 2,. Preparatin and purificatin f 1251-labeled GIP. Purified GIP (stage IV) has been established as a standard. Int several hundred ampules, 6.ug f purified GIP have been aliquted and these have been used in bth the labeling prcedure and fr standard curves. GIP was labeled with by a minr mdificatin f the chlramine T technique. 8 One hundred micrliters f.4 M phsphate buffer, p 7.5, were added t an ampule cntaining 6.ug f GIP. Then rapidly, and in turn, were added 2 mc f (2.uliters), 4.ug f chlramine T (1.uliters), and, after a 15-sec delay, 252.ug f sdium metabisulfite (2.uliters). The steps were perfrmed with air bubbling t ensure rapid and prper mixing. The 15-.uliter reactin vlume was added t a clumn f Sephadex G-15 (.6 by 35 cm). The clumn was eluted with buffer cntaining 2% plasma and 2% Trasyll (FBA Pharmaceuticals, Pinte Claire, Canada) in.2 M acetic acid. Apprximately 4 samples cntaining 3.uliters per tube were cllected (fig. 1). Sephadex G-15 was fund t be superir t G-25 r G-5. It can be seen frm figure 1 that the immunreactivity des nt share cmplete identity with the peak f radiactivity. It has been shwn, by plyacrylamide gel electrphresis, that GIP fragments in the presence f the xidizing agent chlramine T, and these labeled fragments, because f smaller size, elute after the labeled GIP n Sephadex G-15 (unpublished bservatins). The fractins shwing the greatest immunre- 4 I ' 1.6 '" 3 " "'" =.r:;.: 'E ci a. 2 <I> 1 <: " Clumn Fractins - 1 pi Aliquts a. '".r:; '" a..4 " FIG. 1. Separatin f 125I-prcine gastric inhibitry plypeptide (alp) frm free n Sephadex G-15 (35 by.6 cm) with.2 M acetic acid. GIP was eluted in the first peak. Damage assays (e) and binding ability () f the aliquts are als shwn. B/F, bund-free.. mill.
3 March 1974 RADIOIMMUNOASSA Y FOR GIP Cl. '-" c: Cl..2.. 'if.6 \ \.4 \. Q. GIP Standard Lbel Tube # 6 /I /I #9 /I /I.---- mju. O.O r _r ,, GIP Cncentratin (pg./5 jjl. ) r, "r " 2 '25 I - GIP (C.PM. x1 3 ) FIG. 2. Standard curve t gastric inhibitry plypeptide (alp) (e---e) and cmparisn f fractins ns. 6 (--),9 (A--A), and 13 (Ll--Ll) frm labeling prcedure. Variatins f cunts per minute, frm 2 x 1' t ', were added frm each fractin. The tracer cncentratin, 1 x 1' cunts per min. ffractin # 6 was fitted t the standard curve and ther bund-free '''I-prcine GIP fitted accrdingly. There is gd crrelatin between the standard prcine GIP and labeled GIP. activity and least damage were used in the assay. The prer immunreactivity f the fractins preceding the first peak culd be due t increased substitutins f in the GIP mlecule. The percentage f radiactivity crrespnding t 1 25 I_GIP was calculated at apprximately 8%. The specific activity f 125I_GIP using this figure and neglecting damage was calculated t be 27 mc per mg. In figure 2, a curve, prduced when bund-free ratis frm dilutins flabeled and unlabeled prcine GIP were cmpared, is shwn. The 125I_GIP was almst as cmpletely identified by the antiserum as was unlabeled GIP. At high cncentratins f 1251_GIP, there was an apparent slight increase in bund-free ratis ver that fund frm unlabeled GIP. The fractins 6, 9, and 13 are equivalent t fractins 11, 14, and 18 f the chrmatgram shwn in figure 1. Cnditins f incubatin. The diluent buffer was made up as fllws:.4 M phsphate buffer (p 6.5), utdated human plasma (1%) extracted 18 hr with 1 % charcal at 4 C and shwing n detectable GIP n radiimmunassay and Trasyll (5%). Standard curves have been set up in which the cncentratin f Trasyll in the diluent buffer was, 2.5, 5., and 1%. Best results were btained with 5.% Trasyll and this cncentratin has been used in the diluent buffer thrughut the assay. The buffer was used in all dilutins and in crrecting the final incubatin vlume t 5 tlliters. The final vlume f the incubatin mixture fr the standard curves cnsisted f 1 tlliters f antiserum at an initial dilutin 1: 4, final dilutin 1: 2,, 1 tlliters f 1251_GIP cntaining 6 cunts per min, 1 tlliters f standard GIP. range 5 t 25 pg per 1 tlliters, and 2 tlliters f diluent buffer. Where test sera were being assayed, in place f 1 tlliters f GIP standard, 1 tlliters f serum were used, and diluent buffer was added t adjust the final vlume t 5 tlliters. An Oxfrd Pipettr was used thrughut. All assays were set up in triplicate, in silicnized glass culture tubes 1 by 75 mm, and then gently mixed n a Vrtex mixer. Incubatins were perfrmed at 4 C fr 48 hr r 72 hr befre separatin f the bund and free cmpnents. The 72-hr assay had a 24-hr preincubatin and a 48-hr pstincubatin after additin f the 125I_GIP. A 48-hr equilibrium assay at 4 C has been fund t be cmparable t the 72-hr assay. wever, incubatins f less than 48 hr were unsatisfactry, and "damage" was cnsidered excessive after incubatins which lasted mre than 72 hr. Separatin f bund and free GIP. Three
4 36 KUZIO ETAL. Vl. 66, N.3 separatin techniques have been tried using dixane, duble antibdy, and dextran-cated charcal. The dextran-cated charcal was superir t charcal alne, prducing the best separatin, and has been utilized in the assay. Dextran-cated charcal was prepared 24 hr befre use. The buffer (.4 M phsphate, p 6.5 with 2% human plasma and 1% Trasyll) was cled t 4 C prir t the additin f the dextran (T 7, Pharmacia Ltd, Uppsala, Sweden). Charcal (carbn declrizing neutral C-17, Fisher Scientific Ltd, Fair Lawn, N. J.) was added t the dextran slutin. The amunts added yielded a final cncentratin f 2.5 mg f charcal and.5 mg f dextran fr each 2 liters f buffer. The mixture was stirred vernight at 4 C. When separatin f the free frm the bund cmpnents was required, 2 liters f the charcal dextran suspensin was added t each tube and left fr 3 min. The tubes were then centrifuged at 2,8 rpm fr 15 min and the r NO GIP added.8 supernatant decanted. The tubes were crked and placed in plastic carrier tubes fr cunting. Bth the decanted prtin cntaining the bund plypeptide and the charcal dextran, t which was adsrbed the free plypeptide, were cunted in an autmatic l' cunter. Sensitivity and precisin f assay. The cncentratins f prcine GIP in the standard slutins ranged frm 5 t 25 r 5 pg. Antiserum frm guinea pig GP 76 was used at a 1: 2, final dilutin and was the antiserum used thrughut this study. The fall in bundfree rati prduced by lo pg was always much greater than when GIP was excluded. wever, fr assay purpses, the slpe between 25 and 25 pg was used (fig. 3). Nnspecific binding was measured in each assay and used in the calculatin f the amunt f immunlgically active GIP in the unknwn samples. Specificity f antisera. Cmparative immunreactivities f mtilin, natural prcine a...6 Q).4 c () I- a.. I x x \ '" (DIlL.2. I 5 1, I GIP (pg) FIG. 3. Standard curve fr immunassay using antiserum GP 76 at a final dilutin f 1: 2,. The ttal vlume f the incubatin mixture was 5 ILliters.
5 March 1974 RADIOIMMUNOASSA Y FOR GIP a.. "'".5.4 ;; ml"-.3 Glwcaqn... Secret... *-- CCK Mtilln.2.1. I r-,...--,----r--,-,----" IO(x)() Weight f Peptides (pg) FIG. 4. Cmparative immunreactivities f prcine gastric inhibitry plypeptide (GIP), synthetic gastrin (15-Leu), synthetic glucagn, pure natural prcine secretin, and chlecystkinin-pancrezymin (CCK), and natural prcine mtilin. The ttal vlume f the incubatin mixture was 5 ILliters. secretin, synthetic glucagn, synthetic human gastrin (15-Leu), CCK-PZ, and 1-14 GIP in crss reactins versus 125I_GIP are shwn in figure 4. N significant crss reactivity culd be detected, when up t 1 ng f the peptides, ther than GIP, were added. Reprducibility. Reprducibility f the assay was checked by assaying serum samples frm tw experiments n different dates. The serum samples were kept frzen at 2 C between the assays. The results are shwn in figure 5. Only tw f the 14 duplicate bservatins differed by mre than 5%. The remainder were within 25%. Dilutin f serum with high GIP. A serum sample frm a patient (Gr), with a calculated serum GIP level f 4.5 ng per ml, was incubated at varius dilutins (with diluent buffer and charcal-extracted serum) and cmpared with a standard curve. The dilutin in diluent buffer at 5 Illiters per 5 Illiters was fitted t the standard curve and the ther dilutins pltted accrdingly. The results are shwn in figure 6. Results CCK-PZ preparatin (batch n ) frm the Gastrintestinal rmne Research Labratry (Stckhlm, Sweden) has been assayed n 1 ccasins. The preparatin was fund t cntain 17 ± 5.9 pg f GIP per 1, pg. Each ampule (75 Ivy dg units with 3 JLg f preparatin) cntained apprximately 32 JLg f GIP. A recent batch f CCK-PZ (batch n )
6 362 KUZIO ETAL. Vl. 66, N.3 16 g 12. '" '"' <l '" :; 8 c 'e 1:: g 4.. '" Dates f Assay 1/11,2/5 1/22,2/ GI P Cncentratin in Assay 2 (pg) FIG. 5. Reprducibility f determinatins n serum samples. The samples were assayed n different dates and stred at -2 C between assays. GIP, gastric inhibitry plypeptide. N '".<:; '" u Q «> \ \ \ \ x \, e, r-, '--2'b---3T"b--4-b'-----,5b pg/5jji. GIP Standard Gr Serum jji/5 jjl FIG. 6. Serum sample (Grl cntaining a high cncentratin f endgenus gastric inhibitry plypeptide (GIP), incubated at varius dilutins with diluent buffer (,,) r charcal-extracted serum (), and fitted t the standard curve (el at the dilutin in diluent buffer cntaining 5 ILliters per 5 ILliters. ElF, bund-free. has been shwn t cntain a much reduced GIP cntent, 25 pg per 1, pg f preparatin. Early mrning serum levels btained frm 48 subjects, after a 12-hr fast, have been determined at 237 ± 14 pg per ml (± SE), the range being 75 t 5 pg per ml. The subjects were within 1% f ideal bdy weight. Seven subjects were fasted vernight. Fasting serum samples were btained at 8: 45 AM. The subjects were then fed a standard breakfast between 9: 15 and 9: 55 AM. The breakfast cnsisted f 4 unces f range juice, 8 unces f whle milk, bacn and tw eggs with hash-brwn ptates, tast with cnserves, and cffee r tea. Serum samples were btained at 3-min intervals fr the fllwing 3 1/2 hr beginning 45 min after the start f the meal. The results are shwn in figure 7. Pure GIP (ne f the standards) was added t charcal-treated serum t yield a final cncentratin f 5 pg per ml. Ten determinatins n bth 1- and 2-JLliter samples yielded values f 54 ± 4 pg per 1 JLliters (mean ± SE) and 1 ± 1 pg per 2 JLliters (mean ± SE). Discussin Antisera t prcine GIP have been raised in guinea pigs by multiple injectins with bth uncnjugated and cnjugated plypeptide. The antisera have been used in the develpment f a sensitive radiim-
7 March 1974 RADIOIMMUNOASSA Y FOR GIP 363 munassay allwing the detectin f GIP in human sera bth in fasting and stimulated situatins. Prcine GIP has been labeled with and a single clumn system using Sephadex G-15 has been used in islatin f labeled plypeptide frm the ther reactants and free 125I. This system wuld nt be expected t separate 125I_GIP frm GIP. wever, the dilutin curves prduced with labeled and unlabeled plypeptide are cmparable. Separatin f labeled and unlabeled GIP using carbxymethylcellulse has nt prduced better material. Dilutin f a patient's serum with either diluent buffer r charcal-extracted serum, and cmparisn with a standard curve, shws parallelism. It wuld appear then that the antiserum used in this study can identify labeled GIP as it des the unlabeled material, and that there is gd crss reactivity between human and prcine GIP. The mean serum level in 48 nrmal subjects after a 12-hr fast was 237 ± 14 pg per ml (mean ± SE). After feeding, the serum levels in 6 nrmal subjects demnstrated an increase abve 1,2 pg per ml within 45 min after the start f the meal. The levels remained elevated abve 1, pg per ml fr apprximately 2 hr, and significantly abve nrmal sme 4 hr later. During the develpment f the assay, the fasting GIP levels have been seen t fall as imprvements in labeling and separatin f bund and free fractins were intrduced. It had earlier been reprted 6 that the partially purified CCK-PZ preparatin (Gastrintestinal rmne Research Labratry) cntained between 1 and 2 p,g f GIP per ampule. This figure was arrived at frm physilgical studies used in fllwing the purificatin f GIP. Emplying the radiimmunassay, it is calculated that each ampule f CCK-PZ (batch n ) cntains 32 p,g f GIP. A recent batch f CCK-PZ (batch n ) has been fund t cntain substantially less GIP-8.3 p,g per ampule. The recent batches f CCK-PZ are prduced by a technique incrprating anther purificatin stage in which GIP appears t be remved. The ability f the antisera t crss-react with ther knwn gastrintestinal plypeptides and glucagn has been tested. N crss reactivity culd be detected when up t 1, pg f gastrin, glucagn, secretin, CCK-PZ, and mtilin were added. The same antisera used in the radiimmunassay fr GIP have recently been emplyed in lcalizatin f GIP cells in the dudenum and jejunum f dg and man, by the use fthe immunflurescence technique. 9 The cell has been tentatively identi I I!!! I - a. - 8 Q t') E., Cf) 4 i t Fasting -Meal I f 225 Time in Minutes after cmmencing Meal FIG. 7. Serum gastric inhibitry plypeptide (GIP) levels frm 6 nrmal subjects after the ingestin f a standard meal (means ± SE). 285
8 364 KUZIO ETAL. Vl. 66, N. 3 fied by electrn micrscpy as the small granule cntaining Dl cell. 1 The cell is situated in the dudenum and jejunum and is distinct frm bth the S cell (secretin) and the L cell (enterglucagn). Prerequisites fr the establishment f a substance as a hrmne include the identificatin f the cell f rigin and a physilgical mechanism fr its release frm such a cell. It wuld appear that the cell f rigin fr GIP has been tentatively identified, and that changes in circulating GIP levels can be bserved by radiimmunassay, after ingestin f a mixed meal. GIP can be cnsidered a serius candidate fr hrmnal status. Current studies include attempts t identify the specific secretaggue fr the release f GIP frm the dudenum and jejunum by the use f radiimmunassay, investigatin int disease states in which GIP may playa rle, and attempts t crrelate bld changes in GIP with cncurrent physilgical changes. The latter is essential befre hrmnal status can be assigned t GIP. REFERENCES 1. Brwn JC Pedersn RA: A multiparameter study n the actin f preparatins cntaining chlecystkinin-pancrezymin. Scand J Gastrenterl 5: , Brwn JC, Pedersn RA, Jrpes JE, et al: Preparatin f highly active entergastrne. Can J Physil Pharmacl 47: , Brwn JC, Mutt V, Pedersn RA: Further purificatin f a plypeptide demnstrating entergastrne activity. J Physil 29:57-64, Brwn JC: A gastric inhibitry plypeptide. I. The amin acid cmpsitin and the tryptic peptides. Can J Bichem 49: , Brwn JC, Dryburgh JR: A gastric inhibitry plypeptide II. The cmplete amin acid sequence. Can J Bichem 49: , Pedersn RA, Brwn JC: Inhibitin f histamine-, pentagastrin-, and insulin-stimulated canine gastric secretin by pure "gastric inhibitry plypeptide". Gastrenterlgy 62:393-4, Parker CW: Nature f immunlgical respnses and antigen-antibdy interactin, Principles f cmpetitive prtein-binding assays. Edited by WD Odell, W Daughaday. Philadelphia, JB Lippinctt, 1971, p unter WM, Greenwd FC: Preparatin f 1311_ labelled human grwth hrmne f high specific activity. Bichem J 89: , Plak JM, Blm SR, Kuzi M, et al: Cellular lcalizatin f gastric inhibitry plypeptide in the dudenum and jejunum. Gut 14: , Vassall G, Capella C, Slcia E: Endcrine cells f the human gastric mucsa. Z Zellfrsch 118:49-67, 1971
A Plasma Humoral Factor of Extrarenal Origin Causing Release of Reninlike Activity in Hypotensive Dogs
A Plasma Humral Factr f Extrarenal Origin Causing Release f Reninlike Activity in Hyptensive Dgs By E. De Vit, C. Wilsn, R. E. Shipley, R. P. Miller, and B. L. Mrtx ABSTRACT Plasma reninlike activity significantly
More informationThe demonstration of lysosomes by the controlled temperature freezing-sectioning method By LUCILLE BITENSKY
205 The demnstratin f lyssmes by the cntrlled temperature freezing-sectining methd By LUCILLE BITESKY (Frm the Department f Pathlgy, Ryal Cllege f Surgens f England, Lincln's Inn Fields, Lndn, W.C. 2)
More informationThyroglobulin levels in serum and saliva of patients with differentiated thyroid carcinoma
Pre, Indian Acad. Sci., Vl. 87 B (Experimental Bilgy-J), N.7, July 1978. pp. 169-1". @ printed in India Thyrglbulin levels in serum and saliva f patients with differentiated thyrid carcinma D SHAH, S DANDEKAR
More informationHepatic and Pulmonary Clearance of Exogenous Vasoactive Intestinal Peptide in the Rat
GASTROENTEROLOGY 77:55-60, 1979 Hepatic and Pulmnary Clearance f Exgenus Vasactive Intestinal Peptide in the Rat CHRISTOPHER S. HUMPHREY, PHILLIP MURRAY, AMIN M. EBEID, and JOSEF E. FISCHER The Department
More informationUrinary Kallikrein in Rats Bred for Their Susceptibility and Resistance to the Hypertensive Effect of Salt
727 Urinary in Rats Bred fr Their Susceptibility and Resistance t the Hypertensive Effect f Salt A New Radiimmunassay fr Its Direct Determinatin OSCAR A. CARRETERO, VASUDEV M. AMIN, TERESA OCHOLIK, ALFONSO
More informationFrequently Asked Questions: IS RT-Q-PCR Testing
Questins 1. What is chrnic myelid leukemia (CML)? 2. Hw des smene knw if they have CML? 3. Hw is smene diagnsed with CML? Frequently Asked Questins: IS RT-Q-PCR Testing Answers CML is a cancer f the bld
More informationMonensin and Extruded Urea-Grain for Range Beef Cows
Mnensin and Extruded Urea-Grain fr Range Beef Cws R. P. Lemenager, F. N. Owens, w. E. Sharp, Merwin Cmptn and Rbert Ttusek Stry in Brief Tw trials were cnducted t evaluate the supplemental value f mnensin
More informationPROTOCOL 1850 Millrace Drive, Suite 3A Eugene, Oregon
PROTOCOL Cmplex II Enzyme Activity Micrplate Assay Kit 1850 Millrace Drive, Suite 3A Eugene, Oregn 97403 MS241 Rev.0 DESCRIPTION Cmplex II Enzyme Activity Micrplate Assay Kit Sufficient materials are prvided
More informationDiabetologia 9 Springer-Verlag 1985
Diabetlgia (1985) 28: 928-932 Diabetlgia 9 Springer-Verlag 1985 Type IV cllagen antigens in serum f diabetic rats: a marker fr basement membrane cllagen bisynthesis D. G. Brcks 1, H. P. Neubauer I and
More informationSOMATOSTATIN IN MUCOSA OF STOMACH AND DUODENUM IN GASTRODUODENAL DISEASE
0016-5085/78/7501 0013$02.00/0 GASTROENTEROLOGY 75:13-19 1978 Cpyright 1978 by the American Gastrenterlgical Assciatin Vl. 75 N.1 Printed in U.S A. SOMATOSTATN N MUCOSA OF STOMACH AND DUODENUM N GASTRODUODENAL
More informationProtocol. Preparation Protocol for the Non-Targeted Vevo MicroMarker Contrast Agent
Prtcl Preparatin Prtcl fr the Nn-Targeted Vev MicrMarker Cntrast Agent System Cmpatibility: This guide cntains instructins and suggestins fr wrk n the Vev2100, VevLAZR, Vev 3100 systems and transducers
More information(From the Laboratories of the International Health Division of The Rockefeller Foundation, New York)
THE QUANTITATIVE DETERMINATION OF INFLUENZA VIRUS AND ANTIBODIES BY MEANS OF RED CELL AGGLUTINATION BY GEORGE K. HLRST, M.D. (Frm the Labratries f the Internatinal Health Divisin f The Rckefeller Fundatin,
More informationMeasurement of Renin Activity in Human Plasma
Measurement f Renin Activity in Human Plasma By Peter T. Pickens, M.B., M.R.C.P., F. Merlin Bumpus, Ph.D., A. Murray Llyd, M.B., M.R.A.C.P., Rbert R. Smeby, Ph.D., and Irvine H. Page, M.D. Many methds
More informationThe estimator, X, is unbiased and, if one assumes that the variance of X7 is constant from week to week, then the variance of X7 is given by
ESTIMATION PROCEDURES USED TO PRODUCE WEEKLY FLU STATISTICS FROM THE HEALTH INTERVIEW SURVEY James T. Massey, Gail S. Pe, Walt R. Simmns Natinal Center fr Health Statistics. INTRODUCTION In April 97, the
More informationM.J. Guthriel, D.G. Wagner2
EFFECT OF SUPPLEMENTAL PROTEIN OR GRAIN ON INTAKE AND UTILIZATION OF MEDIUM QUALITY PRAIRIE HAY BY STEERS M.J. Guthriel, D.G. Wagner2 and D.C. Weakley3 Stry in Brief Sixteen 1,053 lb tw-year-ld Herefrd
More informationPROTOCOL. SOD2 Protein Quantity Microplate Assay Kit. MS746 Rev.0 DESCRIPTION INTRODUCTION
PROTOCOL SOD2 Prtein Quantity Micrplate Assay Kit 1850 Millrace Drive, Suite 3A Eugene, Oregn 97403 MS746 Rev.0 DESCRIPTION SOD2 Prtein Quantity Micrplate Assay Kit Sufficient materials are prvided fr
More informationby Springer-Verlag 1977
Diabetlgia 13, 615-61 (177) Diabetlgia @ by Springer-Verlag 177 Prevalence f Residual B-Cell Functin in Insulin-Treated Diabetics Evaluated by the Plasma C-Peptide Respnse t Intravenus Giucagn C. Hendriksen,
More informationUse of Alkaline Phosphatase for the Analysis of Tannins in Grapes and Red Wines
Use f Alkaline Phsphatase fr the Analysis f Tannins in Grapes and Red Wines DOUGLAS O. ADAMS ~* and JAMES F. HARBERTSON 2 Tannins are the mst abundant class f phenlics in grape berries and are the predminant
More informationFurther Studies on the Influence of Steroids on
INFECTION AND IMMUNITY, Aug. 1973, p. 151-155 Cpyright ( 1973 American Sciety fr Micrbilgy Vl. 8, N. 2 Printed in U.S.A. Further Studies n the Influence f Sterids n Viral Infectin in Mice D. J. GIRON,
More informationInfluence of Endosperm Type On the Nutritive Value Of Grain Sorghum and Corn
Influence f Endsperm Type On the Nutritive Value Of Grain Srghum and Crn c. A. Hibberd, R. Schemm and D. G. Wagner Stry in Brief The nutritive value f several varieties f grain srghum and crn was studied
More informationTREATMENT OF POLYCYTHEMIA VERA
RUSSELL L. HADEN, M.D. Plycythemia vera is characterized by an increase in the number f red bld cells. This disease is insidius in rigin, chrnic, and withut pathgnmnic symptms r physical findings. Early
More informationSTUDIES WITH HUMAN INFLUENZA VIRUS CULTIVATED IN ARTIFICIAL MEDIUM
Published Online: 1 June, 1936 Supp Inf: http://di.rg/1.184/jem.63.6.83 Dwnladed frm jem.rupress.rg n August 13, 218 STUDIES WITH HUMAN INFLUENZA VIRUS CULTIVATED IN ARTIFICIAL MEDIUM BY T. P. MAGILL,
More informationComparison of three parathyroid hormone assays
Ann Clin Bichem 198; 23: 429-433 Cmparisn f three parathyrid hrmne assays JIFR A ISBT Frm the Department f Chemical Pathlgy, St. Gerge's Hspital and Medical Schl, Lndn SW17, UK SUMMARY. The efficiency
More informationTHE PLACENTAL TRANSMISSION OF ANTIBODIES IN THE SKIN SENSITIVE TYPE OF HUMAN ALLERGY
THE PLACENTAL TRANSMISSION OF ANTIBODIES IN THE SKIN SENSITIVE TYPE OF HUMAN ALLERGY BY WILLIAM B. SHERMAN, M.D., STANLEY F. HAMPTON, M.D., ANn ROBERT A. COOKE, M.D. (Frm the Department f Allergy, The
More informationCONSENT FOR KYBELLA INJECTABLE FAT REDUCTION
CONSENT FOR KYBELLA INJECTABLE FAT REDUCTION INSTRUCTIONS This is an infrmed cnsent dcument which has been prepared t help yur Dctr infrm yu cncerning fat reductin with an injectable medicatin, its risks,
More informationRelationship Between Fertility and the Nonprotein Sulfhydryl Concentration of Seminal Fluid in the Thoroughbred Stallion
Relatinship Between Fertility and the Nnprtein Sulfhydryl Cncentratin f Seminal Fluid in the Thrughbred Stallin Frederick M. Haag, D.V.M., * and N. T. Werthessen, Ph.D. IN AN EARLIER REPORT! it was shwn
More informationOUR STUDIES on the seeming loss of
Evaluatin f Bld Vlume Measurement Techniques By JOHN W. REMINGTON, PH.D., AND CARLETON H. BAKEB, PH.D. OUR STUDIES n the seeming lss f plasma frm the circulatin after transfusin and the mbilizatin f plasma
More informationABIOpure TM Total RNA (version 2.0)
ABIOpure TM Ttal RNA (versin 2.0) Bld Extractin Handbk Cat N: M541RP50-B FOR RESEARCH USE ONLY Table f Cntents Cntents Page Kit Cmpnents 3 Precautins 3 Stability & Strage 4 General Descriptin 4 Limitatins
More informationIntestinal Hormones Inhibit: Intestinal Hormones Promote:
1 QUIZ/TEST REVIEW NOTES SECTION 3 INTESTINAL PHASE OF DIGESTION DIGESTIVE SYSTEM CHAPTER 21 I. INTESTINAL PHASE AND FEEDBACK SIGNALS a. Intr Net result f gastric phase is Digestin f prteins in the stmach
More informationSERIAL ASSAYS OF PLASMA GROWTH HORMONE IN TREATED AND UNTREATED ACROMEGALY*
SERIAL ASSAYS OF PLASMA GROWTH HORMONE IN TREATED AND UNTREATED ACROMEGALY* W. M. HUNTER, F. J. GILLINGHAM, P. HARRIS, J. A. KANIS, F. M. McGURK, J. McLELLAND AND J. A. STRONG Medical Research Cuncil Radiimmunassay
More informationMETHOD 8032 ACRYLAMIDE BY GAS CHROMATOGRAPHY
METHOD 8032 ACRYLAMIDE BY GAS CHROMATOGRAPHY 1.0 SCOPE AND APPLICATION 1.1 Methd 8032 is used t determine trace amunts f acrylamide mnmer in aqueus matrices. This methd may be applicable t ther matrices.
More informationTaste-sickness associations in youngrats over varying delays, stimulus, and test conditions
Animal Learning & Behavir 1980, 8(4), 529-533 Taste-sickness assciatins in yungrats ver varying delays, stimulus, and test cnditins GERARD M. MARTIN and W. K. TIMMINS Australian Natinal University, Canberra,
More informationAnemia. There are three types of Anemia that effect EB patients. They are Iron Deficiency, Chronic Inflammatory and Nutritional.
Anemia Mst patients with Recessive Dystrphic Epidermlysis Bullsa are als Anemic. Anemia in EB is caused by many factrs and can becme very severe if nt treated. Anemia means nt having enugh red cells and
More informationHormonal Control of Cardiac Myosin Adenosine Triphosphatase in the Rat
Hrmnal Cntrl f Cardiac Mysin Adensine Triphsphatase in the Rat By Michael J. Rvett, Ake C. Hjalmarsn, Hward E. Mrgan, Michael J. Barrett, and Richard A. Gldstein ABSTRACT Cardiac functin was impaired in
More informationEffect of Food Deprivation on Rat Gastric Somatostatin and Gastrin Release
GASTROENTEROLOGY 1982;82:871-6 Effect f Fd Deprivatin n Rat Gastric Smatstatin and Gastrin Release HERBERT KOOP, EBERHARD SCHWAB, RUDOLF ARNOLD, and WERNER CREUTZFELDT Divisin f Gastrenterlgy and Metablism,
More informationBiology 30S Unit Test Review: Digestion
Bilgy 30S Unit Test Review: Digestin Test utline: Multiple Chice: 10 Questins, 1 mark each. Shrt Answer: 5 Questins (answer 3 f them), 5 marks each. Lng Answer: 2 Lng Answer Questins, 10 marks each. Tasks
More informationEffects of compost maturity on growth of komatsuna (brassica rapa var. pervidis) in neubauer's pot
Sil Science and Plant Nutritin ISSN: 0038-0768 (Print) 1747-0765 (Online) Jurnal hmepage: https://www.tandfnline.cm/li/tssp20 Effects f cmpst maturity n grwth f kmatsuna (brassica rapa var. pervidis) in
More information* Manuscript received August 25, t Horticultural Research Section, CSIRO, Glen Osmond, South Australia.
SUCROSE BREAKDOWN AND SYNTHESIS IN THE RIPENING GRAPE BERRY* By P. J. HARDyt Sucrse is the majr translcated sugar in the grapevine (Swansn and EIShishiny 1958), but in the berry glucse and fructse tgether
More informationMETHYL TESTOSTERONE IN PREMATURE INFANTS
METHYL TESTOSTERONE IN PREMATURE INFANTS BY URSULA JAMES and B. L. COLES Frm the General Lying-In and Annie McCall Hspitals, Lndn (RECE:IVED FOR PUBLICATiN crber 18. 195 1) It is an established fact that
More informationM.R.C.Path. causes to the raised plasma urea in patients admitted
Pstgradcuate Medical Jurnal (January 1979) 55, 1-14 The cause f the raised plasma urea f acute heart failure R D THOMAS MRCP D B MORGAN MRCPath ALISON NWILL AIMLS Departments f Cardilgy and Chemical Pathlgy,
More informationROLE OF REDUCING CO-FACTOR IN CERULENIN-INSENSIT1VITY OF 6-HYDROXryMELLEIN SYNTHASE IN CARROT CELL EXTRACT
Vl. 46, N. 6, December ]998 BIOCHEMISTRY and MOLECULAR BIOLOGY INTERNATIONAL Pages 1219-1225 ROLE OF REDUCING CO-FACTOR IN CERULENIN-INSENSIT1VITY OF 6-HYDROXryMELLEIN SYNTHASE IN CARROT CELL EXTRACT Fumiya
More informationTHE RABBIT maintained on a highcholesterol
Fatty Acid Cmpsitin f Serum and Artic Intimal Lipids in Rabbits Fed Lw- and High-Chlesterl Diets By DONALD B. ZILVEKSMIT, PH.D., CHARLES C. SWEELEY, PH.D., AND HOWARD A. I. NEWMAN, PH.D. THE RABBIT maintained
More informationSQA-VISION VALIDATION REPORT
Objective SQA-VISION VALIDATION REPORT T test the new cnvenience features f the SQA-Visin vs. the SQA-V and manual semen assessment: Swim-up Density Gradient Lngevity Vitality Mrphlgy Cunter Scanning Debris/Rund
More informationPlasma converting enzyme activity during the development and maintenance of experimental and spontaneous in rats
Atlanta University Center DigitalCmmns@Rbert W. Wdruff Library, Atlanta University Center ETD Cllectin fr AUC Rbert W. Wdruff Library 12-1-1976 Plasma cnverting enzyme activity during the develpment and
More informationHuman-Isotype-Specific Enzyme Immunoassay for Antibodies to Pneumococcal Polysaccharides
JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 1988, p. 1575-1579 Vl. 26, N. 8 0095-1137/88/081575-05$02.00/0 Cpyright D 1988, American Sciety fr Micrbilgy Human-Istype-Specific Enzyme Immunassay fr Antibdies
More informationA pre-conference should include the following: an introduction, a discussion based on the review of lesson materials, and a summary of next steps.
NAU Mdel Observatin Prtcl The mdel prtcl was develped with supprt and expertise frm the Natinal Institute fr Excellence in Teaching (NIET) and is based in great part n NIET s extensive experience cnducting
More informationThe Digestive System. May 16, The Digestive System.notebook
3 The Digestive System.ntebk The Digestive System THE BASIC CONCEPTS Animals btain energy by breaking fd mlecules int smaller pieces. The basic fuel mlecules are amin acids, lipids and carbhydrates. The
More informationEffect of Bombesin on Lower Esophageal Sphincter Pressure in Humans
GASTROENTEROLOGY 1982;83:10-4 Effect f Bmbesin n Lwer Esphageal Sphincter Pressure in Humans ENRICO CORAZZIARI, GIANFRANCO DELLE FA VE, CLAUDIO POZZESSERE, ANNA KOHN, LAURA DE MAGISTRIS, FIORELLA ANZINI,
More informationDIRECTED FORGETIING: SHORT-TERM MEMORY OR CONDITIONED RESPONSE? WENDY S. MILLER and HARVARD L. ARMUS The University of Toledo
The Psychlgical Recrd, 1999, 49, 211-220 DIRECTED FORGETIING: SHORT-TERM MEMORY OR CONDITIONED RESPONSE? WENDY S. MILLER and HARVARD L. ARMUS The University f Tled Previus researchers have interpreted
More informationVITAPRO. Detoxification properties of Apple pectin formulation, PROPECTIN. CENTRE D ETUDE ET DE VALORISATION DES ALGUES FINAL REPORT.
CENTRE D ETUDE & DE VALORISATION DES ALGUES 2400 Detxificatin prperties f Apple pectin frmulatin, PROPECTIN. VITAPRO FINAL REPORT PrPectin July 2015 CENTRE D ETUDE ET DE VALORISATION DES ALGUES PRESQU
More informationVariation in Tissue Carnitine Concentrations with Age and Sex in the Rat
Bichem. J. (1978) 176, 677-681 Printed in Great Britain 677 Variatin in Tissue Carnitine Cncentratins with Age and Sex in the Rat By PEGGY R. BORUM Divisin fnutritin, Department fbichemistry, Vanderbilt
More informationProgramme of Learning. Physical Education. Key Stage 4 Year 10 BTEC Sport
Prgramme f Learning Physical Educatin Key Stage 4 Year 10 BTEC Sprt BTEC Sprt Level 2 Unit 1Fitness fr Sprt and Exercise... 2 Learning aim A: Knw abut the cmpnents f fitness and the principles f training...
More informationYou may have a higher risk of bleeding if you take warfarin sodium tablets and:
MEDICATION GUIDE Warfarin (WAR-far-in) Sdium (SO-dee-um) Tablets USP The 7.5 mg tablets cntain FD&C Yellw N. 5 (tartrazine), which may cause allergic-type reactins (including brnchial asthma) in certain
More informationEffect of Stage of Maturity on the Chemical Composition and In Vitro Digestibility of Sorghum Grain
Effect f Stage f Maturity n the Chemical Cmpsitin and In Vitr Digestibility f Srghum Grain C.A. Hibberd, D.G. Wagner and R.L. Hintz Stry in Brief Dwarf Redlan (waxy), Redlan (nrmal) and Darset (bird-resistant)
More informationReference ID:
HIGHLIGHTS OF PRESCRIBING INFORMATION These highlights d nt include all the infrmatin needed t use CHIRHOSTIM safely and effectively. See full prescribing infrmatin fr CHIRHOSTIM. CHIRHOSTIM (human secretin)
More informationDATA RELEASE: UPDATED PRELIMINARY ANALYSIS ON 2016 HEALTH & LIFESTYLE SURVEY ELECTRONIC CIGARETTE QUESTIONS
DATA RELEASE: UPDATED PRELIMINARY ANALYSIS ON 216 HEALTH & LIFESTYLE SURVEY ELECTRONIC CIGARETTE QUESTIONS This briefing has been specifically prepared fr the Ministry f Health t prvide infrmatin frm this
More informationDiabetologia 9 Springer-Verlag 1984
Diabetlgia (1984) 27:392-396 Diabetlgia 9 Springer-Verlag 1984 12SI-glucagn-degrading activity in acid-saline extracts f rat salivary gland M. Tminaga, K. Yamatani, S. Marubashi, H. Kaneda, H. Manaka,
More informationA RECENT INVESTIGATION 7 of methods for determination of ovulation suggested
The Relatin f Urine V lume t the Menstrual Cycle and Ovulatin WAYNE L. RYAN, PH.D., ROBERT J. LUBY, M.D., AND MICHAEL J. HALLER, A.B. A RECENT INVESTIGATION 7 f methds fr determinatin f vulatin suggested
More informationPediatric and adolescent preventive care and HEDIS *
Pediatric and adlescent preventive care and HEDIS * * HEDIS is a registered trademark f the Natinal Cmmittee fr Quality Assurance (NCQA). UniCare Health Plan f West Virginia, Inc. Healthcare Effectiveness
More informationIntegrity of Energy Stores in Cat Papillary Muscle
Integrity f Energy Stres in Cat Papillary Muscle EFFECT OF CHANGES IN TEMPERATURE AND FREQUENCY OF NTRACTION ON HIGH ENERGY PHOSPHATE STORES By Peter E. Pl, M.D., Brian M. Chandler, M.D., Edmund H. Snnenblick,
More informationRuminal Availability of Phosphorus and Its Effect on Digestion
Table 2. Trial results Cncentratin f Captan,ppm liem 0 160 320 640 Dry matter digestibility (%) Ruminal 65.1 72.8 69.4 74.3 Ttal 82.1 81.9 82.4 82.0 Starch digestin (%) Ruminal 87.7 93.6 89.3 92.2 N digestibility
More informationEffect of Pancreatic Juice and Trypsin on Oleic Acid-Stimulated Pancreatic Secretion and Plasma Secretin in Dogs
GASTROENTEROLOGY 1989;96:1330-6 Effect f Pancreatic Juice and Trypsin n Oleic Acid-Stimulated Pancreatic Secretin and Plasma Secretin in Dgs K. SHIRATORI, Y. H. JO, K. Y. LEE, T. M. CHANG, and W. Y. CHEY
More information1.6. Topic 1: Cell Biology (Teacher) Essential Idea: Cell division is essential but must be controlled. 1.6 Cell Division
Tpic 1: Cell Bilgy (Teacher) 1.6 Essential Idea: Cell divisin is essential but must be cntrlled. 1.6 Cell Divisin Why d cells divide: - Sa:Vl Rati - Allws fr grwth f the rganism - Allws fr cell differentiatin
More informationAn Official Journal of the American Heart Association
Circulatin Research OCTOBER VOL. XXXI An Official Jurnal f the American Heart Assciatin Increased Plasma Aldsterne Cncentratin in Respnse t Hemdialysis in Nephrectmized Man By Rbert E. McCa, Cnnie S. McC,
More informationASSESSMENT OF PITUITARY FUNCTION IN PATIENTS WITH SERUM PROLACTIN LEVELS GREATER THAN 100 NG/ML*t
FERTILITY AND STERILITY Cpyright 1979 The American Fertility Sciety Vl. 32, N.2, August 1979 Printed in U.s.A. ASSESSMENT OF PITUITARY FUNCTION IN PATIENTS WITH SERUM PROLACTIN LEVELS GREATER THAN 100
More informationSimultaneous Determination of Urinary
Simultaneus Determinatin f Urinary Ascrbic Acid and Creatinine by Highperfrmance Liquid Chrmatgraphy (Accepted n Mar. 26, 1996) Yshihir Shimada Sanae K Key wrds : Urinary Ascrbic Acid, Creatinine, High-perfrmance
More informationName: Date: Period: Notes: The Blood and Lymphatic System
Name: Date: Perid: Cmpsitin f Bld and their Functins Red Bld Cells (aka ) Structure Ntes: The Bld and Lymphatic System D nt have a like ther cells d Cntain a specialized prtein called Hemglbin cntains
More informationExtraction of oleic acid from jojoba oil, soybean oil and olive oil Phase diagrams
IndianJurnalfChemicalTechnlgy Vl.3, Nvember1996,pp. 299-305 Extractin f leic acid frm jjba il, sybean il and live il Phase diagrams JaimeWisniak*,AlexanderApelblat&Ahu-AkelKhaled epartmentfchemicalengineering,ben-gurianuniversityf
More informationDiphtheria-Tetanus-Acellular Pertussis-Hepatitis B-Polio-Haemophilus Influenzae type b Conjugate Combined Vaccine Biological Page (DTaP-IPV-Hib-HB)
Diphtheria-Tetanus-Acellular Pertussis-Hepatitis B-Pli-Haemphilus Influenzae type b Cnjugate Cmbined Vaccine Bilgical Page (DTaP-IPV-Hib-HB) Sectin 7: Bilgical Prduct Infrmatin Standard #: 07.214 Created
More informationEffects of Leukotrienes on Gastric Acid and Alkaline Secretions
GASTRONTROLOGY 1987;92:1209-14 ffects f Leuktrienes n Gastric Acid and Alkaline Secretins S. J. KONTURK, J. BILSKI, A. DMBINSKI, A. WARZCHA, G. BCK, and H. JNDRALLA Institute f Physilgy, Academy f Medicine,
More informationFDA Dietary Supplement cgmp
FDA Dietary Supplement cgmp FEBRUARY 2009 OVERVIEW Summary The Fd and Drug Administratin (FDA) has issued a final rule regarding current gd manufacturing practices (cgmp) fr dietary supplements that establishes
More informationAnalysis of Pesticide Residues in Citrus Oils by GCxGC-TOFMS with Minimal Sample Preparation
Analysis f Pesticide Residues in Citrus Oils by GCxGC-TOFMS with Minimal Sample Preparatin LECO Crpratin; Saint Jseph, Michigan USA Key Wrds: GCxGC-TOFMS, Classificatins, Decnvlutin, Quantificatin, Sample
More informationSupporting information
Supprting infrmatin Prteins Human serum albumin frm Sigma (378, fatty acid free, 99% pure) was purified frm dimer and cntaminating prteins using gel filtratin n a 00 x 3.4 cm Sephadex G50 clumn in ammnium
More informationFor Research Use Only Ver
INSTRUCTION MANUAL Quick-cfRNA Serum & Plasma Kit Catalg N. R1059 Highlights High-quality cell-free RNA is easily and rbustly purified frm up t 3 ml f plasma, serum, r ther bilgical fluids. Purified cell-free
More informationSURFACE MODIFICATION AND ELECTRO- PHORESIS OF NORMAL AND TRANSFORMED BHK21 CELLS
J. CM Sci. 14, 3-214 (1974) 3 Printed in Great Britain SURFCE MODIFICTION ND ELECTRO- PHORESIS OF NORML ND TRNSFORMED BHK21 CELLS. L. LTNER ND G.. TURNER Cancer Research Unit, University Department f Clinical
More informationthat of the adult liver. To explore the above deranged flavin-nucleotide metabolism in foetuses consequent on immunoneutralization of the maternal
Bichem. J. (1985) 23, 363-367 Printed in Great Britain 363 Enzymic basis f deranged fetal flavin-nucletide metablism cnsequent n immunneutralizatin f maternal ribflavin carrier prtein in the pregnant rat
More informationP02-03 CALA Program Description Proficiency Testing Policy for Accreditation Revision 1.9 July 26, 2017
P02-03 CALA Prgram Descriptin Prficiency Testing Plicy fr Accreditatin Revisin 1.9 July 26, 2017 P02-03 CALA Prgram Descriptin Prficiency Testing Plicy fr Accreditatin TABLE OF CONTENTS TABLE OF CONTENTS...
More informationSUBNORMAL TESTICULAR FUNCTION IN A BULL CONCEALED BY PHAGOCYTOSIS OF ABNORMAL SPERMATOZOA IN THE EFFERENT DUCTULES
SUBNORMAL TESTICULAR FUNCTION IN A BULL CONCEALED BY PHAGOCYTOSIS OF ABNORMAL SPERMATOZOA IN THE EFFERENT DUCTULES B. CRABO, B. GUSTAFSSON, L. NICANDER and A. R. RAO Departments f Clinical Bichemistry,
More informationSTUDIES ON THE FEASIBILITY OF PREDICTING FEEDLOT PERFORMANCE FROM CERTAIN LABORATORY GRAIN ANALYSES
University f Nebraska - Lincln DigitalCmmns@University f Nebraska - Lincln Faculty Papers and Publicatins in Animal Science Animal Science Department January 1978 STUDIES ON THE FEASIBILITY OF PREDICTING
More informationINTRODUCTION TO THE CIRCULATORY SYSTEM
INTRODUCTION TO THE CIRCULATORY SYSTEM What des bld d? 5. What makes this pssible? : In rder fr there t be an efficient exchange f xygen, waste and nutrients there must be a high surface area between the
More informationHIV Diagnostic Tests. HIV Testing Algorithm at SydPath (National Reference Laboratory)
HIV Diagnstic Tests HIV Testing Algrithm at SydPath (Natinal Reference Labratry) HIV1/2 Ab/Ag Cmbi is Architect HIV- 1 Ab/Ag EIA is Genscreen Sandwich EIA 4 th Generatin HIV Ab/Ag Chemiluminescene Micr
More informationCancer Immunology, Immunotherapy (submitted in 2014) Christian Krug et al.
Cancer Immunlgy, Immuntherapy (submitted in 2014) Christian Krug et al. PBMC day 0 d 0 n anti-cd3 side scatter d 0 with anti-cd3 frward scatter PBMC day 10 d 10 n anti-cd3 side scatter d 10 with anti-cd3
More informationSTUDIES ON THE BIOGENESIS OF SMOOTH ENDOPLASMIC RETICULUM MEMBRANES IN RATS
STUDIES ON THE BIOGENESIS OF SMOOTH ENDOPLASMIC RETICULUM MEMBRANES IN HEPATOCYTES OF PHENOBARBITAL-TREATED RATS II. The Site f Phsphlipid Synthesis in the Initial Phase f Membrane Prliferatin JOAN A.
More informationCampus Climate Survey
Campus Climate Survey Executive Summary www.ecu.edu/ecyu 2016 A prject spnsred by the Office fr Equity and Diversity Executive Summary Prject Backgrund In FY 2013-2014, the Campus Climate Cmmissin prpsed
More informationFILTRATION AND REABSORPTION OF PROTEIN BY THE KIDNEY* BY ALVIN L. SELLERS, M.D., NEILYN GRIGGS, JESSIE MARMORSTON, M.D., AND HOWARD C. GOODMAN, M.D.
Published Online: 1 July, 1954 Supp Inf: http://di.rg/1.184/jem.1.1.1 Dwnladed frm jem.rupress.rg n August 21, 218 FILTRATION AND REABSORPTION OF PROTEIN BY THE KIDNEY* BY ALVIN L. SELLERS, M.D., NEILYN
More information* CORRESPO~~ING AUTHOR. YAWS/PMVH/jtf-88 IN CENTRAL AFRICA IMMUNOFLUORESCENCE FOR DIAGNOSIS GONZALEZ. MARTIN. M.H. GEORGES-COURBOT M.C. PALISSON. M.J.
YAWS/PMVH/jtf-88 YA~S IN CENTRAL AFRICA CLINICAL ASPECTS AND USEFULNESS OF INDIRECT ABSORBED IMMUNOFLUORESCENCE FOR DIAGNOSIS MARTIN. P.M.V.* GONZALEZ. J.P. MARTIN. M.H. GEORGES-COURBOT M.C. PALISSON.
More informationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
HYPOGLYCEMIC ACTION OF 2-AMINONORBORNANE-2-CARBOXYLIC ACID IN THE RAT* H. S. Tager and H. N. Christensen Department f Bilgical Chemistry, The University f Michigan Ann Arbr, Michigan 48104 Received May
More informationDiabetologia 9 by Springer-Verlag 1979
Diabetlgia 16, 365-372 (1979) Diabetlgia 9 by Springer-Verlag 1979 Interrelatinships f Glucse and Insulin Uptake by Muscle f Nrmal and Diabetic Man vidence f a Difference in Metablism f ndgenus and xgenus
More informationBIOLOGY 101. CHAPTER 7: Membrane Structure and Function: Life at the Edge
BIOLOGY 101 CHAPTER 7: Membrane Structure and Functin: Life at the Edge Membrane Structure and Functin: Life at the Edge CONCEPTS: 7.1 Cellular membranes are fluid msaics f lipids and prteins 7.2 Membrane
More informationKetone-Body Utilization by Adult and Suckling Rat Brain in vivo
Bichem. J. (1971) 122, 13-18 13 Printed in Great Britain Ketne-Bdy Utilizatin by Adult and Suckling Rat Brain in viv By R. A. HAWKINS, D. H. WILLIAMSN AND H. A. KREBS Metablic Research Labratry, Nuffield
More informationDivision of Nuclear Medicine Procedure / Protocol
Divisin f Nuclear Medicine Prcedure / Prtcl GASTRIC EMPTYING CPT CODE: 78264 UPDATED: FEBRUARY 2013 Indicatins: Patient Prep: Scheduling: This examinatin: Is indicated in patients with diabetes and thse
More information1.11 INSULIN INFUSION PUMP MANAGEMENT INPATIENT
WOMEN AND NEWBORN HEALTH SERVICE CLINICAL GUIDELINES SECTION A: GUIDELINES RELEVANT TO OBSTETRICS AND GYNAECOLOGY 1 STANDARD PROTOCOLS 1.11 INSULIN INFUSION PUMP MANAGEMENT - INPATIENT Authrised by: OGCCU
More informationbinding by solubilized components of the zona pellucida
Sperm binding t the pig zna pellucida and inhibitin f binding by slubilized cmpnents f the zna pellucida Trish Berger, Arlene Davis, N. J. Wardrip and Jerry L. Hedrick Departments f * Animal Science and
More informationgoitre who present local compression symptoms.
Radiidine therapy in vluminus multindular nn-txic gitre Jean Vereist, Marc Bnnyns, and Daniel Gliner Departments f Internal Medicine and Radiistpes, University Hspital Saint-Pierre, Brussels, Belgium Abstract.
More informationBiochi~ic~a et Biophysica A~ta
ELSEVIER Bichimica et Biphysica Acta 1195 (1994) 21-30 BB Bichi~ic~a et Biphysica A~ta Factrs influencing the retentin and chemical stability f ply(ethylene glycl)-lipid cnjugates incrprated int large
More informationPlaque Formation by Influenza Viruses in the Presence of Trypsin
J. gen. Virl. (I974), 25, 351-357 Printed in Great Britain 35I Plaque Frmatin by Influenza Viruses in the Presence f Trypsin By G. APPLEYARD AND H. B. MABER Micrbilgical Research Establishment, Prtn, Nr
More informationISOLATION AND CHARACTERIZATION OF FOUR PEPTIDE HYDROLASES FROM THE CYTOSOL OF RAT INTESTINAL MUCOSA
GASTROENTEROLOGY 72:93-100, 1977 Cpyright 1977 by The Williams & Wilkins C. Vl. 72, N.1 Printed in U.S.A. ISOLATION AND CHARACTERIZATION OF FOUR PEPTIDE HYDROLASES FROM THE CYTOSOL OF RAT INTESTINAL MUCOSA
More informationEURL-SRM - Analytical Observations Report
cncerning the fllwing EURL-SRM - Analytical Observatins Reprt Cmpund(s): Pymetrzine Cmmdities: Varius cmmdities f plant rigin Methd(s): QuEChERS, adjustment f ph Instrumentatin: LC-MS/MS Analysis f Pymetrzine
More informationMetabolism: Oxidative Phosphorylation
Metablism: Oxidative Phsphrylatin Hand-ut fr the CBT versin April 2012 1. On average, hw much ATP is needed each day by ur bdy? 2. Which f the fllwing ccurs during cellular respiratin? Bisynthesis f glycgen
More informationDiphtheria-Tetanus-Acellular Pertussis-Hepatitis B-Polio-Haemophilus Influenzae type b Conjugate Combined Vaccine Biological Page (DTaP-IPV-Hib-HB)
Diphtheria-Tetanus-Acellular Pertussis-Hepatitis B-Pli-Haemphilus Influenzae type b Cnjugate Cmbined Vaccine Bilgical Page (DTaP-IPV-Hib-HB) Sectin 7: Bilgical Prduct Infrmatin Standard #: 07.214 Created
More information