A HemoCue Novel Technology Product for 5-Part Differentiation of Leukocytes Point-of-Care

Size: px
Start display at page:

Download "A HemoCue Novel Technology Product for 5-Part Differentiation of Leukocytes Point-of-Care"

Transcription

1 A HemoCue Novel Technology Product for -Part Differentiation of Leukocytes Point-of-Care Stellan Lindberg, M Sc Medical Director HemoCue AB Kuvettgatan 1 SE Ängelholm Sweden Stellan.Lindberg@hemocue.se

2 HemoCue WBC DIFF System - the first -part Diff WBC counter for POCT 1. Fill the microcuvette with 1 µl blood 2. Push the button for patient test and place the microcuvette in the cuvette holder 3. The results will be displayed within five minutes

3 A Photo Microscope is used to detect the stained white cells Chemistry in the microcuvette: Saponin Haemolyses the red blood cells Methylene Blue stains the nucleus of the white cell Volume appr..2 µl Cuvette chamber with a fixed thickness and a defined detection area gives a fixed volume for enumerations of stained cells The volume is well specified by having: a specific measurement area of the microcuvette tight tolerances of the cavity depth in the microcuvette

4 The cavity is analyzed in separate layers to enable detection of cells at different depths Cavity depth 14 µm The camera lens moves in small steps taking several images through the cavity of the cuvette All cells in all images will be cut out Identifying when each cell is in focus Mount the focused cells into one image Count WBC and differentiate on this final image

5 Photoshoping of each cell in the images Neutrophils Lymphocytes Monocytes Transferring characteristics into mathematical algorithms Eosinophils Basophils WBC DIFF uses over 3 features and state-of-the-art image analysis technology

6 WBC DIFF can identify and flag pathological cells Sample with 87, % blast cells Sample with 2, % myelocytes and 4,3 % metamyelocytes

7 Prediction model object Half Sensitivity and specificity Pathological classification tree Normal cells classification tree Basophils Blurred Trash Multiple Debris Eosinophils Neutrophils Lymphocytes Pathology flag Sensitivity: 83.2 % Specificity: 96.1 % Meta Myelo Blast cells Monocytes Sensitivity abnormal morphology: 88.1 % Ref: CLSI H2-A2 Pathology flag part diff

8 Advanced Internal QC System 1. At power up ( Self test ): Test of electronics and software Blanking test (Quality of light, check for dirt in the optics) 2. Between samples: Blanking test 3. For each sample: Correct sampling detection Bad area detection Out of focus detection Air bubble detection Poor light intensity Bad cell distribution

9 The results from WBC DIFF correlate well to common laboratory cell counters Total Leukocytes Neutrophils Lymphocytes Monocytes Eosinophils Siemens ADVIA y =.93x -.17 r =.993 n = y =.87x +.2 r =.994 n = y =.96x +.18 r =.94 n = y = 1.2x -.16 r =.69 n = 11 1, 1,, y =.92x +.2 r =.76 n = Leukocytes x1 9 /L Siemens ADVIA Neutrophils x1 9 /L ADVIA Lymphocytes x1 9 /L ADVIA Monocytes x1 9 /L ADVIA 212,,, 1, 1, Eosinophils x1 9 /L ADVIA 212 Beckman Coulter LH y =.98x -.23 r =.996 n = Leukocytes x1 9 /L Beckman Coulter LH y =.94x +.2 r =.994 n = Neutrophils x1 9 /L Beckman Coulter LH y =.99x +.1 r =.96 n = Lymphocytes x1 9 /L Beckman Coulter LH y =.63x +.1 r =.8 n = Monocytes x1 9 /L Beckman Coulter LH7 1, 1,, y = 1.13x +, r =.86 n = 84,,, 1, 1, Eosinophils x1 9 /L Beckman Coulter LH7 Sysmex XS-1i y =.97x -.18 r =.997 n = y =.98x +. r =.989 n = y = 1.1x -.1 r =.98 n = 92 x19/l HemoCue WBC DIFF y =.1x +.1 r =.76 n = 92 1,, y = 1.11x -.2 r =.91 n = Leukocytes x1 9 /L Sysmex XS-1i 1 1 Neutrophils x1 9 /L Sysmex XS-1i Lymphocytes x1 9 /L Sysmex XS-1i Monocytes x19/l Sysmex XS-1i,,, 1, Eosinophils x1 9 /L Sysmex XS-1i

10 WBC DIFF at POC fulfills a clinical unmet need in several care settings and clinical applications Emergency clinic We conclude that for clinicians who evaluate patients with a suspected severe infection in the ED, special attention should be directed to the RR, CRP, and WBC, and that low Hb may be an underestimated biomarker in this context Gille-Johnson P., et al., Scand. Journal of Infectious Diseases (212) In an emergency care setting, both lymphocytopenia and NLCR are better predictors of bacteremia than routine parameters like CRP level, WBC count and neutrophil count. Attention to these markers is easy to integrate in daily practice and without extra costs. de Jager C.P., et al., Crit Care 14 (21) Doctor s office In conclusion, the NLCR (Neutrophil to Lymphocyte Count Ratio) may serve as a simple marker for discrimination between severe bacterial and viral infections. Holub M. et al., Cent. Eur. J. Med. (212) Pediatric clinic White blood cell count can aid judicious antibiotic prescribing in acute upper respiratory infections in children Casey, J. et al, Clin Pediatr 42 (23) Psychiatric clinic Monitoring total WBC and neutrophils (NEU) with a point-of-care device during Clozapine treatment on schizophrenic patients Nielsen, J., et al., Eur. Neuropsychopharmacol. (211) Oncology clinic Monitoring before next doze of chemotherapy Price CP, et al, Point-of-care testing; 2nd ed. (24)

11 Conclusion The novel POCT HemoCue WBC DIFF technology is built on state-of-the art imaging technology The results from the system are accurate and precise and correlate well to laboratory cell counters both in the hands of lab technicians as well as untrained users A white blood cell count including a -part diff at the point of care will increase the availability of already well established and frequently used lab parameters Rapid and easy access will be a valuable tool for physicians in making direct and more well informed decisions in several clinical conditions

12 Acknowledgements: Jönsson, Inger and Nilsson, Magnus, Växjö Central Hospital Clin Chem Dept, Växjö, Sweden Wahlström, Saga, Malmö University Hospital, Clin Chem Dept, Malmö, Sweden 1 Jonasson-Bjäräng, Tomas and Johnsson, Elin, HemoCue AB, Ängelholm, Sweden 1 ) Saga Wahlström has sadly passed away since the study was done, the data has been published by permission from her clinic in Malmö

Meet the HemoCue WBC DIFF

Meet the HemoCue WBC DIFF Meet the HemoCue WBC DIFF THE CHALLENGE The frequent challenge in any patient assessment is for clinician to assess, test, diagnose and conclude treatment all in just one consultation session. More often

More information

Full Blood Count analysis Is a 3 part-diff good enough? Dr Marion Münster, Sysmex South Africa

Full Blood Count analysis Is a 3 part-diff good enough? Dr Marion Münster, Sysmex South Africa Full Blood Count analysis Is a 3 part-diff good enough? Dr Marion Münster, Sysmex South Africa The Role of the FBC in clinical decision making History Examination Investigations Decision 70% FBC Laboratory

More information

WELCOME! HemoCue Presentation CPOCT San Diego, September 20 th Annika Eriksson Global Application Manager HemoCue AB

WELCOME! HemoCue Presentation CPOCT San Diego, September 20 th Annika Eriksson Global Application Manager HemoCue AB WELCOME! HemoCue Presentation CPOCT San Diego, September 20 th 2014 Annika Eriksson Global Application Manager HemoCue AB HemoCue Part of the Danaher Family 2 Pioneers in Point of care Testing When HemoCue

More information

Determination of hemoglobin is one of the most commonly

Determination of hemoglobin is one of the most commonly ORIGINAL ARTICLE Multiple-Site Analytic Evaluation of a New Portable Analyzer, HemoCue Hb 201+, for Point-of-Care Testing Sten-Erik Bäck, PhD,* Carl G. M. Magnusson, PhD, Lena K. Norlund, MD, PhD, Henning

More information

Sue Jung Kim, MD, MS; Yoonjung Kim, MD; Saeam Shin, MD; Jaewoo Song, MD, MS; Jong Rak Choi, MD, PhD

Sue Jung Kim, MD, MS; Yoonjung Kim, MD; Saeam Shin, MD; Jaewoo Song, MD, MS; Jong Rak Choi, MD, PhD Comparison Study of the Rates of Manual Peripheral Blood Smear Review From 3 Automated Hematology Analyzers, Unicel DxH 800, ADVIA 2120i, and XE 2100, Using International Consensus Group Guidelines Sue

More information

Getting Beyond the Flags: Quantitative assessment of immature granulocyte (IG) populations may improve the assessment of sepsis and inflammation.

Getting Beyond the Flags: Quantitative assessment of immature granulocyte (IG) populations may improve the assessment of sepsis and inflammation. Getting Beyond the Flags: Quantitative assessment of immature granulocyte (IG) populations may improve the assessment of sepsis and inflammation. Sysmex America White Paper One Nelson C. White Parkway,

More information

ADVANCED HAEMATOLOGY BATTLE OF THE BANDS. Dennis B. DeNicola, DVM, PhD, DACVP IDEXX Laboratories, Inc. Westbrook, Maine, USA BACKGROUND

ADVANCED HAEMATOLOGY BATTLE OF THE BANDS. Dennis B. DeNicola, DVM, PhD, DACVP IDEXX Laboratories, Inc. Westbrook, Maine, USA BACKGROUND ADVANCED HAEMATOLOGY BATTLE OF THE BANDS Dennis B. DeNicola, DVM, PhD, DACVP IDEXX Laboratories, Inc. Westbrook, Maine, USA BACKGROUND The identification of immature neutrophils (bands, metamyelocytes,

More information

Customer Information Literature List White Blood Cells

Customer Information Literature List White Blood Cells Customer Information Literature List White Blood Cells Date: September 2015 Subject: Literature List White Blood Cells Issued by: Scientific Customer Services Number: 150901 Note: Whether references are

More information

WHITE PAPER IMMATURE GRANULOCYTE (IG) POPULATIONS MAY IMPROVE THE ASSESSMENT OF INFECTION AND SEPSIS. Getting beyond the flags.

WHITE PAPER IMMATURE GRANULOCYTE (IG) POPULATIONS MAY IMPROVE THE ASSESSMENT OF INFECTION AND SEPSIS. Getting beyond the flags. WHITE PAPER IMMATURE GRANULOCYTE (IG) POPULATIONS MAY IMPROVE THE ASSESSMENT OF INFECTION AND SEPSIS. Getting beyond the flags www.sysmex.com/us Introduction For many decades, physicians have relied on

More information

Northern Territory Point-of-Care Testing Program

Northern Territory Point-of-Care Testing Program Northern Territory Point-of-Care Testing Program Brooke Spaeth, NT POCT Program Coordinator Flinders University International Centre for Point-of-Care Testing Dr Rodney Omond, NT POCT Program Clinical

More information

Literature List Body Fluids

Literature List Body Fluids Literature List Body Fluids Customer Information March 2018 Date: March 2018 Subject: Literature List body fluids Issued by: Scientific Customer Services Number: 180312 Note: Whether references are given

More information

A Look Into the Determination of Cell Morphology in Hematology in the 21 st Century. Ramon Simon-Lopez, MD Global Scientific Director Beckman Coulter

A Look Into the Determination of Cell Morphology in Hematology in the 21 st Century. Ramon Simon-Lopez, MD Global Scientific Director Beckman Coulter A Look Into the Determination of Cell Morphology in Hematology in the 21 st Century Ramon Simon-Lopez, MD Global Scientific Director Beckman Coulter Is cell morphology important? AML M7 CLL CD5 CD19 NHL

More information

Guide to the 1-3 Minute Blood Film Microscopic Review: Why and How?

Guide to the 1-3 Minute Blood Film Microscopic Review: Why and How? Guide to the 1-3 Minute Blood Film Microscopic Review: Why and How? Dennis B. DeNicola, DVM, PhD, DACVP Chief Veterinary Educator IDEXX Laboratories, Inc. Westbrook, ME USA Adjunct Professor of Veterinary

More information

Evaluation of the Diagnostic Performance of the Sysmex XT-2000i Automated Hematology Analyzer in the Detection of Immature Granulocytes

Evaluation of the Diagnostic Performance of the Sysmex XT-2000i Automated Hematology Analyzer in the Detection of Immature Granulocytes Evaluation of the Diagnostic Performance of the Sysmex XT-2000i Automated Hematology Analyzer in the Detection of Immature Granulocytes A. M. CENCI *1, M. MACONI *2, and B. CASOLARI *1 *1 Laboratory of

More information

Performance evaluation of Celltac G: a new automated hematology analyzer

Performance evaluation of Celltac G: a new automated hematology analyzer Original Article Performance evaluation of Celltac G: a new automated hematology analyzer Masaaki Sugiyama 1, Tsukasa Kobayashi 1, Yuki Jisyage 2, Shigeko Yamamoto 2, Yutaka Nagai 2,3 and Hiroshi Kondo

More information

Pseudobasophilia in Pediatric Age Group

Pseudobasophilia in Pediatric Age Group Original Article DOI: 10.17354/ijss/2015/259 Pseudobasophilia in Pediatric Age Group Femela Muniraj 1, Vijay Amritraj 2 1 Assistant Professor, Department of Pathology, Chettinad Hospital & Research Institute,

More information

Performance Evaluation of the CellaVision DM96 System WBC Differentials by Automated Digital Image Analysis Supported by an Artificial Neural Network

Performance Evaluation of the CellaVision DM96 System WBC Differentials by Automated Digital Image Analysis Supported by an Artificial Neural Network Hematopathology / DIGITAL IMAGE ANALYSIS IN HEMATOLOGY Performance Evaluation of the CellaVision DM96 System WBC Differentials by Automated Digital Image Analysis Supported by an Artificial Neural Network

More information

HumaCount 5D. Outperforming 5-part Hematology System. CoreLab DX. Hematology

HumaCount 5D. Outperforming 5-part Hematology System. CoreLab DX. Hematology HumaCount 5D Outperforming 5-part Hematology System Direct Capillary Process by OptimalCount Technology Distinct 5-part Differentiation Definite Immature Cell Count (LIC, ALY) CoreLab DX Hematology 5-part

More information

CELL-DYN Strength in Technology, Proven Reliability. Optical WBC Technology. Patented M.A.P.S.S. Differential. Multiple Technologies

CELL-DYN Strength in Technology, Proven Reliability. Optical WBC Technology. Patented M.A.P.S.S. Differential. Multiple Technologies CELL-DYN 3700 Strength in Technology, Proven Reliability Optical WBC Technology Patented M.A.P.S.S. Differential Multiple Technologies CELL-DYN 3700 Multiple Technologies One Superior Result Multiple Technologies

More information

Interpreting Hematology Scatter-Plots; One Cancer Center s Keys to Seeing the BIG Picture

Interpreting Hematology Scatter-Plots; One Cancer Center s Keys to Seeing the BIG Picture Interpreting Hematology Scatter-Plots; One Cancer Center s Keys to Seeing the BIG Picture Barbara L. Burch, MHA MT (ASCP) Laboratory Manager New York University Clinical Cancer Center Disclosure Ms Burch

More information

Differential Blood Smear H3

Differential Blood Smear H3 Verein für Association pour le Associazione per il medizinische Qualitätskontrolle contrôle de qualité médical controllo di qualità medico Report Differential Blood Smear H3 MQ 2015-4 MQ, Institut für

More information

Blutbild 2012: Automatisiert oder manuell? Georg Stüssi Servizio di Ematologia Istituto Oncologico della Svizzera Italiana

Blutbild 2012: Automatisiert oder manuell? Georg Stüssi Servizio di Ematologia Istituto Oncologico della Svizzera Italiana Blutbild 2012: Automatisiert oder manuell? Georg Stüssi Servizio di Ematologia Istituto Oncologico della Svizzera Italiana IS THERE STILL A ROLE FOR THE MANUAL BLOOD COUNT? Indication for examination

More information

XN series. Case interpretation. Gebruikersdag Vlaanderen- 6 oktober 2016

XN series. Case interpretation. Gebruikersdag Vlaanderen- 6 oktober 2016 XN series Case interpretation Gebruikersdag Vlaanderen- 6 oktober 2016 Fluorescence flow cytometry RET channel PLT-F channel WDF channel WPC channel WNR channel Case 1 Case 1: Initial measurement Patient

More information

Notes for the 2 nd histology lab

Notes for the 2 nd histology lab Notes for the 2 nd histology lab Note : Please refer to the slides and see the morphological characteristics of each cell, as the practical exam will be in the form of figures. SLIDE #2 Erythropoiesis

More information

Sysmex Journal International Vol.26 No.1 (2016)

Sysmex Journal International Vol.26 No.1 (2016) Sysmex Journal International Vol.26 No.1 (216) Performance Evaluation of the XN-55 Automated Hematology Analyzer Body Fluid Mode Considerations for Operational Conditions for Cell Counting with Cerebrospinal

More information

We noted a problem with the use of the hematology

We noted a problem with the use of the hematology Standard Article J Vet Intern Med 2017;31:1708 1716 Feline Differential Leukocyte Count with ProCyte Dx: Frequency and Severity of a Neutrophil-Lymphocyte Error and How to Avoid It H.W. Tvedten, V. Andersson,

More information

1 BIO 212: ANATOMY & PHYSIOLOGY II PLATELETS. Mature Stage: No nucleus. Only 2-3 µm in diameter: significantly smaller than RBCs

1 BIO 212: ANATOMY & PHYSIOLOGY II PLATELETS. Mature Stage: No nucleus. Only 2-3 µm in diameter: significantly smaller than RBCs 1 BIO 212: ANATOMY & PHYSIOLOGY II LAB BLOOD PLATES EOSINOPHIL Contains large red-staining granules Usually 2 lobes 12-17 µm: about the size of neutrophils (2X erythrocytes) regulation/reduction of Histamine.

More information

MLAB 1211 Body Fluids BF Differentials - pretest

MLAB 1211 Body Fluids BF Differentials - pretest MLAB 1211 BF Differentials - pretest Instructor: Carolyn Ragland MLAB 1211 pre-test #2 Spring No Extra Materials Needed Name Date Instructions: Neatly write your answer in the space provided. Unless otherwise

More information

By Dr. Mohamed Saad Daoud

By Dr. Mohamed Saad Daoud By Dr. Mohamed Saad Daoud Part I Introduction Types of White Blood Cells Genesis of the White Blood Cells Life Span of the White Blood Cells Dr. Mohamed Saad Daoud 2 Leucocytes Introduction: Infectious

More information

Hematology 101. Cindy Rogers, MT(ASCP) Diagnostics System Specialist

Hematology 101. Cindy Rogers, MT(ASCP) Diagnostics System Specialist Hematology 101 Cindy Rogers, MT(ASCP) Diagnostics System Specialist More Acronyms...» CBC» RBC» HGB» HCT» WBC» MPV» PLT» RDW» DIFF» H&H» Complete Blood Count» Red Blood Cell» Hemoglobin» Hematocrit» White

More information

Tube 1 : FLAER/CD24/CD16/CD15/CD45

Tube 1 : FLAER/CD24/CD16/CD15/CD45 Gating procedure for white blood cells (WBC) on FC500 The French PNH diagnosis working group has developed an optimized gating strategy adapted to a two-tubes diagnostic test (PMN, MO), evaluated in a

More information

Cbc with differential

Cbc with differential Cbc with differential Other tests might be necessary based on the results of the differential test and follow-up tests. Your doctor has many ways of determining and treating causes of abnormal blood cell

More information

Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Laboratory locations: 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK - Cheltenham Hospital Hatherley Lane Cheltenham GL51 6SY Contact: Chris Watson Tel: +44 (0) 1242 246516 E-Mail: chris.watson@nuffieldhealth.com

More information

Combining. and New Diagnostic. to Help Clinicians Achieve. Patient Outcomes at. per Healthcare Encounter

Combining. and New Diagnostic. to Help Clinicians Achieve. Patient Outcomes at. per Healthcare Encounter Combining and New Diagnostic to Help Clinicians Achieve Patient Outcomes at per Healthcare Encounter Holly McDaniel, MD hmcdaniel@clinpath.com Holly.mcdaniel@bannerhealth.com Holly McDaniel, MD AP/CP and

More information

Flow cytometry leukocyte differential : a critical appraisal

Flow cytometry leukocyte differential : a critical appraisal Flow cytometry leukocyte differential : a critical appraisal Francis Lacombe Flow cytometry department University Hospital of Bordeaux, Pessac, France francis.lacombe@chu-bordeaux.fr 2008 HORIBA ABX, All

More information

Validation of Criteria for Smear Review Following Automated Blood Cell Analysis in Ain Shams University Laboratory

Validation of Criteria for Smear Review Following Automated Blood Cell Analysis in Ain Shams University Laboratory Validation of Criteria for Smear Review Following Automated Blood Cell Analysis in Ain Shams University Laboratory Azza S Eldanasoury 1, Noha H Boshnak 2, Raghda E Abd El Monem 3 1 MD, Professor of Clinical

More information

Blood DLC, Retic count, PCV, Hb and ESR. Dr. Tamara Alqudah

Blood DLC, Retic count, PCV, Hb and ESR. Dr. Tamara Alqudah Blood DLC, Retic count, PCV, Hb and ESR Dr. Tamara Alqudah Differential Leukocyte Count (DLC) There are 5 main types of WBCs: 1. Neutrophils: 40-80% 2. Eosinophils: 1-6 % 3. Basophils: < 1-2% 4. Lymphocytes:

More information

Changes of hematological references depends on storage period and temperature conditions in rats and dogs

Changes of hematological references depends on storage period and temperature conditions in rats and dogs ISSN 1738-6055 (Print) ISSN 2233-7660 (Online) Lab Anim Res 2016: 32(4), 241-248 https://doi.org/10.5625/lar.2016.32.4.241 Changes of hematological references depends on storage period and temperature

More information

INFECTION/ INFLAMMATION

INFECTION/ INFLAMMATION HAEMATOLOGY OCTOBER 2017* WHITE PAPER INFECTION/ INFLAMMATION Novel haematological parameters for rapidly monitoring the immune system response Patients with inflammatory disease are common on hospital

More information

Leukocytosis. dr. Erdélyi, Dániel 2 nd Department of Paediatrics Semmelweis University

Leukocytosis. dr. Erdélyi, Dániel 2 nd Department of Paediatrics Semmelweis University Leukocytosis dr. Erdélyi, Dániel 2 nd Department of Paediatrics Semmelweis University My first day at work in 1997 3y with fever, cough Is this bronchitis, pneumonia, pharyngitis, sinusitis, else? Is this

More information

Leukocytosis - Some Learning Points

Leukocytosis - Some Learning Points Leukocytosis - Some Learning Points Koh Liang Piu Department of Hematology-Oncology National University Cancer Institute National University Health System Objectives of this talk: 1. To provide some useful

More information

Biochemical and Hematological Markers of Inflammation Accurately Predict Sepsis and its Severity in ICU Patients

Biochemical and Hematological Markers of Inflammation Accurately Predict Sepsis and its Severity in ICU Patients Sysmex Journal International Volume 29 No.1 (2019) Published 8 March, 2019 Original Article ISSN 2434-7825 Biochemical and Hematological Markers of Inflammation Accurately Predict Sepsis and its Severity

More information

Differential Blood Smear H3

Differential Blood Smear H3 Verein für Association pour le Associazione per il medizinische Qualitätskontrolle contrôle de qualité médical controllo di qualità medico Report Differential Blood Smear H3 MQ 2018-1 MQ, Institut für

More information

Impact of Integrating Rumke Statistics to Assist with Choosing Between Automated Hematology Analyzer Differentials vs Manual Differentials

Impact of Integrating Rumke Statistics to Assist with Choosing Between Automated Hematology Analyzer Differentials vs Manual Differentials Impact of Integrating Rumke Statistics to Assist with Choosing Between Automated Hematology Analyzer Differentials vs Manual Differentials Laura Stephens, 1 Wendy Hintz-Prunty, 1 Hans-Inge Bengtsson, 2

More information

EDUCATIONAL COMMENTARY DIFFERENTIATING IMMATURE PERIPHERAL BLOOD CELLS

EDUCATIONAL COMMENTARY DIFFERENTIATING IMMATURE PERIPHERAL BLOOD CELLS Educational commentary is provided through our affiliation with the American Society for Clinical Pathology (ASCP). To obtain FREE CME/CMLE credits click on Continuing Education on the left side of the

More information

Autoanalyzer Generated Spurious Basophilia in Adolescents and Adults

Autoanalyzer Generated Spurious Basophilia in Adolescents and Adults Original Article DOI: 10.17354/ijss/2015/294 Autoanalyzer Generated Spurious Basophilia in Adolescents and Adults Femela Muniraj 1, Vijay Amritraj 2, Govindaraju Soundararajan 3 1 Assistant Professor,

More information

Precision and Accuracy of the Leukocyte Differential on the Sysmex XE-2100

Precision and Accuracy of the Leukocyte Differential on the Sysmex XE-2100 Precision and Accuracy of the Leukocyte Differential on the Sysmex XE-2100 R. HERKLOTZ and A. R. HUBER Zentrum für Labormedizin, Kantonsspital Aarau, 5001 Aarau, Switzerland. The automated leukocyte(wbc)

More information

Lab 1 Blood Composition and formed elements

Lab 1 Blood Composition and formed elements Lab 1 Blood Composition and formed elements Plasma 55% of whole blood 90% water 8% proteins from liver 2% misc. Nutrients: AA, glucose, lipids vitamins, minerals Wastes: urea, uric acid, creatine, ammonium

More information

Hematopoiesis Simplified: Part 1 Erythropoiesis

Hematopoiesis Simplified: Part 1 Erythropoiesis Hematopoiesis Simplified: Part 1 Erythropoiesis Larry Johnson Texas A&M University Hematopoiesis Simplified: Part 1 Erythropoiesis Objectives are to: Identify the developmental cells of erythropoiesis

More information

Complete Blood Count PSI AP Biology

Complete Blood Count PSI AP Biology Complete Blood Count PSI AP Biology Name: Objective Students will examine how the immunological response affects molecules in the blood. Students will analyze three complete blood counts and create diagnoses

More information

Evaluation of the Abbott CELL-DYN 4000 Hematology Analyzer

Evaluation of the Abbott CELL-DYN 4000 Hematology Analyzer Hematopathology / EVALUATION OF THE ABBOTT CELL-DYN 4 HEMATOLOGY ANALYZER Evaluation of the Abbott CELL-DYN 4 Hematology Analyzer Ernesto Grimaldi, MD, and Francesco Scopacasa, PhD Key Words: Abbott CD

More information

Preechaya Wongkrajang, M.D., Chaicharoen Tantanate, M.D. ABSTRACT

Preechaya Wongkrajang, M.D., Chaicharoen Tantanate, M.D. ABSTRACT OriginalArticle Comparison of Leukocyte Differential Count by Beckman Coulter Unicel DxH800, Beckman Coulter LH780 and Sysmex XE-5000 Preechaya Wongkrajang, M.D., Chaicharoen Tantanate, M.D. Department

More information

System overview Installation System Description System Default Settings and Loading I-Button Tests Components/Kits /Accessories Maintenance & Cleaning

System overview Installation System Description System Default Settings and Loading I-Button Tests Components/Kits /Accessories Maintenance & Cleaning SQA-VISION TRAINING Content of Presentation System overview Installation System Description System Default Settings and Loading I-Button Tests Components/Kits /Accessories Maintenance & Cleaning SQA-Vision

More information

Blood smear analysis in the emergency veterinary patient

Blood smear analysis in the emergency veterinary patient Vet Times The website for the veterinary profession https://www.vettimes.co.uk Blood smear analysis in the emergency veterinary patient Author : Ashley Wemple Categories : RVNs Date : April 1, 2010 Ashley

More information

٢٨/٠١/١٤٣٧. Prof. M. Rushdi.

٢٨/٠١/١٤٣٧. Prof. M. Rushdi. ١ PARAMETERS OF LEUCOCYTES PICTURE TtlWBC Total WBCs count Differential LC Hemocytometer Blood film Blood Cell Counter Blood Cell Counter INTERPRETATION OF LEUCOCYTES PICTURE 1.Leucocytosis. 2. Leucopenia.

More information

Blood Cell Identification Graded

Blood Cell Identification Graded BCP-21 Blood Cell Identification Graded Case History The patient is a 37-year-old female with a history of multiple sickle cell crises. She now presents with avascular necrosis of the left hip. Laboratory

More information

Evaluation of haematology analyzer CELL-DYN 3700 SL

Evaluation of haematology analyzer CELL-DYN 3700 SL Evaluation of haematology analyzer CELL-DYN 3700 SL Enver Suljevi}, Muhamed Fazli}, Jozo ]ori} i Emina Kiseljakovi} Institute for Clinical Chemistry and Biochemistry, Clinical Centre of University of Sarajevo

More information

CELL-DYN 3700 Strength in Technology, Proven Reliability

CELL-DYN 3700 Strength in Technology, Proven Reliability CELL-DYN 3700 Strength in Technology, Proven Reliability Optical WBC Technology Patented MAPSS Differential Multiple Technologies CELL-DYN 3700 Multiple Technologies One Superior Result Multiple Technologies

More information

Blood Cells Med Terms Quiz

Blood Cells Med Terms Quiz Blood Cells Med Terms Quiz Question Prompt: 1 Mononuclear white blood cells (agranulocyte) formed in lymph tissue, also a phagocyte and a precursor of macrophages are leukocytes. True False Question Prompt:

More information

BIOMARKERS IN SEPSIS: DO THEY REALLY GUIDE US? Asist. Prof. M.D. Mehmet Akif KARAMERCAN Gazi University School of Medicine Depertment of Emergency

BIOMARKERS IN SEPSIS: DO THEY REALLY GUIDE US? Asist. Prof. M.D. Mehmet Akif KARAMERCAN Gazi University School of Medicine Depertment of Emergency BIOMARKERS IN SEPSIS: DO THEY REALLY GUIDE US? Asist. Prof. M.D. Mehmet Akif KARAMERCAN Gazi University School of Medicine Depertment of Emergency Medicine 1 NO CONFLICT OF INTEREST 2 We do not fully understand

More information

Hematology 101. Blanche P Alter, MD, MPH, FAAP Clinical Genetics Branch Division of Cancer Epidemiology and Genetics Bethesda, MD

Hematology 101. Blanche P Alter, MD, MPH, FAAP Clinical Genetics Branch Division of Cancer Epidemiology and Genetics Bethesda, MD Hematology 101 Blanche P Alter, MD, MPH, FAAP Clinical Genetics Branch Division of Cancer Epidemiology and Genetics Bethesda, MD Hematocrits Plasma White cells Red cells Normal, Hemorrhage, IDA, Leukemia,

More information

Redefine Performance. BD Veritor. System Revolutionizes Testing at the Point of Care. Fast. Streamlined Workflow Requires minimal hands-on time

Redefine Performance. BD Veritor. System Revolutionizes Testing at the Point of Care. Fast. Streamlined Workflow Requires minimal hands-on time CLIA WAIVED Redefine Performance System BD Veritor System Revolutionizes Testing at the Point of Care Accurate The first CLIA-waived Digital Immunoassay (DIA), a new category of diagnostic tests where

More information

ADx Bone Marrow Report. Patient Information Referring Physician Specimen Information

ADx Bone Marrow Report. Patient Information Referring Physician Specimen Information ADx Bone Marrow Report Patient Information Referring Physician Specimen Information Patient Name: Specimen: Bone Marrow Site: Left iliac Physician: Accession #: ID#: Reported: 08/19/2014 - CHRONIC MYELOGENOUS

More information

Chapter 11. Lecture and Animation Outline

Chapter 11. Lecture and Animation Outline Chapter 11 Lecture and Animation Outline To run the animations you must be in Slideshow View. Use the buttons on the animation to play, pause, and turn audio/text on or off. Please Note: Once you have

More information

EDUCATIONAL COMMENTARY MORPHOLOGIC ABNORMALITIES IN LEUKOCYTES

EDUCATIONAL COMMENTARY MORPHOLOGIC ABNORMALITIES IN LEUKOCYTES EDUCATIONAL COMMENTARY MORPHOLOGIC ABNORMALITIES IN LEUKOCYTES Educational commentary is provided through our affiliation with the American Society for Clinical Pathology (ASCP). To obtain FREE CME/CMLE

More information

Ordering Physician CLIENT,CLIENT. Collected REVISED REPORT

Ordering Physician CLIENT,CLIENT. Collected REVISED REPORT HPWET Hematopathology Consultation, MML Embed Client Hematopathology Consult REVISED INAL DIAGNOSIS Interpretation Peripheral blood, bone marrow aspirate and biopsies, bilateral iliac crests: 1. Normocellular

More information

State of the Art of HbA1c Measurement

State of the Art of HbA1c Measurement State of the Art of HbA1c Measurement XXI Congreso Latinoamericano de Patologia Clinica Y XLII Congreso Mexicano de Patologia Clinica, Cancun October 2012 Randie R. Little, Ph.D. NGSP Network Laboratory

More information

First comparative evaluation of leucocyte differential and reticulocyte count between Abbott Cell -DYN 3700 and Beckman-Coulter Gen S

First comparative evaluation of leucocyte differential and reticulocyte count between Abbott Cell -DYN 3700 and Beckman-Coulter Gen S 111 Riv Med Lab - JLM, Vol. 4, N. 2, 2003 First comparative evaluation of leucocyte differential and reticulocyte count between Abbott Cell -DYN 3700 and Beckman-Coulter Gen S H. Vandeputte a, D. Darre

More information

Presented by: Dr. Giuseppe Molinaro Dr. Davide De Biase

Presented by: Dr. Giuseppe Molinaro Dr. Davide De Biase Presented by: Dr. Giuseppe Molinaro Dr. Davide De Biase Dog Spayed Female LABRADOR RETRIEVER 3 Years old VACCINATIONS ANTIPARASITIC COMMERCIAL DIET VOMITING FOR A MONTH DULLNESS WEIGHT LOSS INAPPETANCE

More information

M. Velizarova, T. Yacheva and K. Tzatchev Department of Clinical Laboratory and Clinical Immunology, MU Sofia

M. Velizarova, T. Yacheva and K. Tzatchev Department of Clinical Laboratory and Clinical Immunology, MU Sofia 50, 48, 2012, 3, ADVIA 2120.,.., ANALYSIS OF DIAGNOSTIC RED CELL CYTOGRAMS, GENERATED BY THE ADVIA 2120 HEMATOLOGY ANALYZER M. Velizarova, T. Yacheva and K. Tzatchev Department of Clinical Laboratory and

More information

Flow Cytometry. What is flow cytometry?

Flow Cytometry. What is flow cytometry? Flow Cytometry Flow Cytometry What is flow cytometry? Flow cytometry is a popular laser-based technology to analyze the characteristics of cells or particles. It is predominantly used to measure fluorescence

More information

Medical School Histology Basics. VIBS 289 lab. Blood

Medical School Histology Basics. VIBS 289 lab. Blood Medical School Histology Basics VIBS 289 lab Blood Larry Johnson Texas A&M University Blood (definition and function) Blood - fluid tissue composed of erythrocytes (RBC), leukocytes (WBC), and platelets

More information

Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Haematology & Blood Transfusion Contact: Neil Wrathall The Christie Tel: +44 (0) 161 918 7264 Wilmslow Road Fax: +44 (0) 161 446 8549 Manchester

More information

Optimization of Criteria for Verification of Automated Platelet Counts Generated by the Sysmex XE-2100 Hematology Analyzer

Optimization of Criteria for Verification of Automated Platelet Counts Generated by the Sysmex XE-2100 Hematology Analyzer Optimization of Criteria for Verification of Automated Platelet Counts Generated by the Sysmex XE-2100 Hematology Analyzer Science Gene Gulati, PhD, Sean Cote, DO, Eric Behling, MD, William Kocher, MD

More information

Blood. Kris*ne Kra.s, M.D.

Blood. Kris*ne Kra.s, M.D. Blood Kris*ne Kra.s, M.D. The most beau*ful thing we can experience is the mysterious. It is the source of all true art and science. - Albert Einstein Blood Lecture Objec*ves Be able to iden*fy and

More information

Differential Blood Smear H3

Differential Blood Smear H3 Verein für Association pour le Associazione per il medizinische Qualitätskontrolle contrôle de qualité médical controllo di qualità medico Report Differential Blood Smear H3 MQ 2014-2 MQ, Institut für

More information

Acta Medica Okayama. Studies on diagnosis of leukemia by tissue culture. Kiyoshi Hiraki APRIL Volume 12, Issue Article 8

Acta Medica Okayama. Studies on diagnosis of leukemia by tissue culture. Kiyoshi Hiraki APRIL Volume 12, Issue Article 8 Acta Medica Okayama Volume 12, Issue 1 1958 Article 8 APRIL 1958 Studies on diagnosis of leukemia by tissue culture Kiyoshi Hiraki Okayama University, Copyright c 1999 OKAYAMA UNIVERSITY MEDICAL SCHOOL.

More information

Flow Cytometric White Blood Cell Differential Using CytoDiff is Excellent for Counting Blasts

Flow Cytometric White Blood Cell Differential Using CytoDiff is Excellent for Counting Blasts Original Article Diagnostic Hematology Ann Lab Med 215;35:28-34 http://dx.doi.org/1.3343/alm.215.35.1.28 ISSN 2234-386 eissn 2234-3814 Flow Cytometric White Blood Cell Differential Using is Excellent for

More information

Requirements: Glass slides Leishman stain Microscopes Disposable needles Vials containing anticoagulants Methylated-spirit Staining rack

Requirements: Glass slides Leishman stain Microscopes Disposable needles Vials containing anticoagulants Methylated-spirit Staining rack Aim: To make a blood smear and to count the different types of leucocytes present in a stained blood smear and express their relative counts in percentage Principle - The blood contains various types of

More information

The white blood cell differential (WBC-diff) technologies

The white blood cell differential (WBC-diff) technologies The New Hematology Analyzer Sysmex Performance Evaluation of a Novel White Blood Cell Differential Technology Katharina Ruzicka, MD; Mario Veitl, MD; Renate Thalhammer-Scherrer, MD; Ilse Schwarzinger,

More information

HISTOLOGY VIRTUAL LABORATORY BLOOD AND LYMPHATICS SYSTEM

HISTOLOGY VIRTUAL LABORATORY BLOOD AND LYMPHATICS SYSTEM HISTOLOGY VIRTUAL LABORATORY BLOOD AND LYMPHATICS SYSTEM Login: http://histopath.westernu.edu Histology Atlas AND Virtual Histology links. I. HEMATOLOGY - PERIPHERAL BLOOD Purpose: To be able to identify

More information

HEMOTOLOGY. B. Helps stabilize body temperature -heats up and cools down slowly which moderates body temp

HEMOTOLOGY. B. Helps stabilize body temperature -heats up and cools down slowly which moderates body temp I. Body H 2 O = HEMOTOLOGY A. Variable quantities 1. sweating and urination ( ) decreases H 2 O 2. drinking H 2 O increases B. Water is found in two compartments 1. contains 2/3 of all water in your body

More information

Forum for Collaborative HIV Research External Validation of CD4 and Viral Load Assays Paris, France June 29, 2007

Forum for Collaborative HIV Research External Validation of CD4 and Viral Load Assays Paris, France June 29, 2007 Forum for Collaborative HIV Research External Validation of CD4 and Viral Load Assays Paris, France June 29, 2007 Thomas J. Spira, M.D. International Laboratory Branch Global AIDS Program Centers for Disease

More information

The challenges of quality assessment for point-of-care testing. Anne Stavelin. EQALM Symposium, Bergen 8 October General challenges

The challenges of quality assessment for point-of-care testing. Anne Stavelin. EQALM Symposium, Bergen 8 October General challenges The challenges of quality assessment for point-of-care testing Anne Stavelin EQALM Symposium, Bergen 8 October 2015 www.noklus.no Norwegian quality improvement of primary care laboratories General challenges

More information

Med Chem 535P ~ Diagnostic Medicinal Chemistry. General Comments

Med Chem 535P ~ Diagnostic Medicinal Chemistry. General Comments Med Chem 535P ~ Diagnostic Medicinal Chemistry General Comments Most blood chemistry and serology assays are performed automatically. Larger clinical laboratories often use sophisticated analyzers that

More information

Composition of Blood

Composition of Blood Blood is a connective tissue, specialized to transport the respiratory gasses as well as hormones, nutrients, and wastes, and the distribution of heat. The various cells of the blood perform specific functions.

More information

CIRCULATION & GAS EXCHANGE

CIRCULATION & GAS EXCHANGE AP BIOLOGY ACTIVITY2.13 Text:Campbell,v.8,chapter42 NAME DATE HOUR CIRCULATION & GAS EXCHANGE 1. In general, what is the function of transport systems? 2. What method/structure do most invertebrates use

More information

LYDIA NELSON, M.S., MT(ASCP), SH, SAMUEL CHARACHE, M.D., SANDRA WINGRELD, MT(ASCP), AND EDWARD KEYSER, MT(ASCP)

LYDIA NELSON, M.S., MT(ASCP), SH, SAMUEL CHARACHE, M.D., SANDRA WINGRELD, MT(ASCP), AND EDWARD KEYSER, MT(ASCP) Laboratory Evaluation of Differential White Blood Cell Count nformation from the S-Plus V and H-1 in Patient Populations Requiring Rapid "Turnaround" Time LYDA NELSON, M.S., MT(ASCP), SH, SAMUEL CHARACHE,

More information

PointCare NOW TM Technical Note

PointCare NOW TM Technical Note PointCare NOW TM Technical Note Introduction to our Technology And Summary of Evaluation and Performance data 1 of 19 Introduction The PointCare NOW is a portable, and very fast, diagnostic instrument

More information

Offending agents like: 1. Infectious agents: 2. toxic agents(toxins) Micro-organisms Bacteria Viruses Parasites Fungi

Offending agents like: 1. Infectious agents: 2. toxic agents(toxins) Micro-organisms Bacteria Viruses Parasites Fungi Immunity Dr.Talar Immunity: is the ability of the human body to resist almost all types of offending agents that tend to damage the tissues and organs or it s a special system for combating different infectious

More information

Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Laboratory locations: 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Blood Sciences Pathology Building Hull Royal Infirmary Anlaby Road Hull HU3 2JZ Contact: Dr Andrew Botham Tel: +44 (0)1482

More information

g/dl g/dl

g/dl g/dl Bio 151 Laboratory Manual Human Anatomy & Physiology II #3 - Objectives: Determine the hemoglobin content of sheep blood Know the normal values of hemoglobin for males and females Be able to calculate

More information

Literature List White Blood Cells

Literature List White Blood Cells Literature List White Blood Cells Customer Information February 2018 Date: February 2018 Subject: Literature List White Blood Cells Issued by: Scientific Customer Services Number: 180202 Note: Whether

More information

Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Schedule of ccreditation United Kingdom ccreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK 20 June 2017 ccredited to Haematology & Blood Tranfusion Craigavon rea Hospital

More information

DEPARTMENT OF PHYSIOLOGY

DEPARTMENT OF PHYSIOLOGY UNIVERSITY OF MEDICAL SCIENCES, ONDO DEPARTMENT OF PHYSIOLOGY BLOOD AND BODY FLUID PHYSIOLOGY LECTURER: MR A.O. AKINOLA OBJECTIVES Leukopoiesis Thrombopoiesis Leukopoiesis and Lymphopoiesis White blood

More information

Detail PRINCIPLE: Body fluids other than blood and urine will be analyzed according to their site of origin, and the providers specific orders.

Detail PRINCIPLE: Body fluids other than blood and urine will be analyzed according to their site of origin, and the providers specific orders. Subject Body Fluid Analysis - Affiliate Index Number Lab-8760 Section Laboratory Subsection Regional/Affiliates Category Departmental Contact Munson, Karen Last Revised 6/28/2017 References Required document

More information

Test Bulletin. Save time, money, resources, and precious blood by NOT collecting extra specimens just in case

Test Bulletin. Save time, money, resources, and precious blood by NOT collecting extra specimens just in case Test Bulletin October 2018 Save time, money, resources, and precious blood by NOT collecting extra specimens just in case ACL Laboratories has seen an increase in the number of specimens submitted without

More information

The Complete Blood Count

The Complete Blood Count The Complete Blood Count (Cartesian Thinking at Its Best) A SEM Image of Normal Human Blood Laurie Larsson February 22, 2010 Anatomy and Philology II Dr. Danil Hammoudi Introduction A complete blood count

More information

AP2 Lab 1 - Blood & Heart

AP2 Lab 1 - Blood & Heart AP2 Lab 1 - Blood & Heart Project 1 - Formed Elements Identification & Recognition See fig. 17.10 and Table 17.2. Instructor may also provide other images. Note: See Fig. 17.11 All formed elements are

More information