Insulin Aspart ELISA Kit

Size: px
Start display at page:

Download "Insulin Aspart ELISA Kit"

Transcription

1 Insulin Aspart ELISA Kit Catalogue DEIABL215 For the qualitative determination of antibodies to insulin aspart in human serum and plasma FOR RESEARCH USE ONLY Creative Diagnostics. All rights reserved Ramsey Road Shirley, NY 11967, USA Tel: Fax:

2 INTRODUCTION Insulin aspart (Novolog / Novorapid ) is a fast-acting insulin analog with an earlier onset of action than regular human insulin. It is produced through recombinant DNA technology so that the amino acid, B28, which is normally proline, is substituted with an aspartic acid residue. This analog has increased charge repulsion, which prevents the formation of hexamers, to create fasteracting insulin. The sequence is inserted into the yeast genome, and the yeast expressed the insulin analog, which is then harvested from a bioreactor. Insulin aspart is indicated to improve glycemic control in adults and children with diabetes mellitus. Due to the fast acting nature it, the dosage of insulin aspart must be individualized. When it is given by subcutaneous injection, it should generally be used in regimens with an intermediate or long-acting insulin Because of NovoLog s comparatively rapid onset and short duration of glucose lowering activity, some patients may require more basal insulin and more total insulin to prevent pre-meal hyperglycemia when using insulin aspart than when using human regular insulin. In various clinical trials, insulin aspart has been reported to generate an antibody response. Increases in anti-insulin antibody titers that react with both human insulin and insulin aspart have been observed in patients treated with NovoLog. Increases in anti-insulin antibodies are observed more frequently with NovoLog than with regular human insulin. Data from a 12- month controlled trial in patients with type 1 diabetes suggest that the increase in these antibodies is transient. The formation of antibodies to a therapeutic agent may decrease the efficacy of these agents, leading to a loss of clinical response over time. In some cases, anti-drug antibodies may cause infusion reactions and serious anaphylactic reactions. Detection, measurement and characterization of anti-therapeutic antibodies are critical in understanding the safety, exposure and efficacy profile of the therapeutic agent. The CD Insulin aspart ADA ELISA kit is designed for the quantitative determination of antibodies to Insulin aspart in serum and plasma. Our ELISA-based kits combine a fast, user-friendly format with a sensitive and specific assay. The assay is designed to detect human IgG, IgM and IgA against Insulin aspart. 2

3 PRINCIPLE OF THE ASSAY This assay employs the sandwich enzyme immunoassay technique. The therapeutic antibody is coated onto a 96-well microplate. Quality control (QC) samples and test samples are pipetted into the appropriate wells. Anti- therapeutic antibodies present in biological matrices are bound by the immobilized therapeutic protein. After washing away any unbound substances, enzyme conjugated detection antibody is added to the wells. The plate is washed to remove any unbound enzyme conjugated reagent and a substrate solution is added to the wells for colour development. The colour development is proportional to the amount of anti-therapeutic antibody present in test samples. The colour development is stopped and its intensity is measured. SAFETY PRECAUTIONS 1. The test protocol must be followed strictly. 2. All reagents containing human material should be handled as if potentially infectious. Operators should wear gloves and protective clothing when handling any patient sera or serum based products. 3. The kit reagents contain antimicrobial agents, acid and 3,3, 5,5 -tetramethylbenzidine. Avoid contact with the skin and eyes. Rinse immediately with plenty of water if any contact occurs. 4. Any liquid brought into contact with potentially infectious material needs to be discarded in a container with a disinfectant. Disposal must be performed in accordance with local regulations. 5. Only trained laboratory personnel should execute this test. 3

4 MATERIALS & STORAGE Store kit components at 2 to 8 C unless specified otherwise. DO NOT USE past kit expiration date. Some vials contain a small amount of reagent. Spin tubes on pulse setting prior to opening. Do not mix or substitute reagents with those from other lots. Each kit includes: Coated Microtiter Plate(s) (1x8 strips) Coating Protein (1500X) Positive Control Concentrate Control Diluent* Detection Reagent (1000X)* Coating Buffer (10X) Blocking Buffer Wash Buffer (20X) Assay Buffer TMB TMB Stop Solution * Store at -15 to -30⁰C upon receipt. Avoid repeated freeze-thaw. Materials and instruments required but not supplied: Microplate reader ( nm) Plate shaker Distilled or de-ionized water Disposable tips Precision pipettes ( μl) Vortex mixer Multi-channel pipette ( μl) Adhesive plate sealers PREPARATION OF REAGENTS Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label. 1. Coating Buffer (1X) Preparation: Dilute coating buffer concentrate with ultra-pure water 1/10 before use (for example, add 1.2 ml concentrate to 10.8 ml of ultra-pure water for a final volume of 12 ml). Mix well. Do not store excess solution. 4

5 2. Coating Solution (1X) Preparation: Dilute coating protein concentrate with prepared coating buffer 1/1500 before use (for example, add 8 µl concentrate to ml of prepared coating buffer for a final volume of 12 ml). Mix well. Do not store excess solution. 3. Wash Buffer (1X) Preparation: Dilute wash buffer concentrate with ultra-pure water 1/20 before use (for example, add 1.25 ml concentrate to ml of ultra-pure water for a final volume of 25 ml). Mix well. Store at 2 to 8 C for up to 1 week. 4. Preparation of Positive Controls: a. High Positive Control Preparation (RECOMMENDED): Dilute positive control concentrate with control diluent 1/2 (for example, add 50 µl of concentrate to 50 µl of control diluent for a final volume of 100 µl). Mix well. Prepare fresh on the day of use. b. Low Positive Control Preparation (RECOMMENDED): Dilute positive control concentrate with control diluent 1/20 (for example, add 5 µl of concentrate to 95 µl of control diluent for a final volume of 100 µl). Mix well. Prepare fresh on the day of use. 5. Detection Reagent (1X) Preparation: Dilute detection reagent concentrate with assay buffer 1/1000 before use (for example, add 12 µl of concentrate to ml of assay buffer for a final volume of 12 ml). Mix well. Prepare fresh on day of use. 5

6 SPECIMEN COLLECTION & STORAGE This kit is compatible with EDTA, heparin or citrate plasma, and serum samples. Samples can be stored at or below -20 C for up to 1 year. Serum: To collect serum, use a serum separator tube and allow the whole blood to clot for minutes. Then centrifuge blood for 15 minutes at 1000 x g and remove serum immediately. Plasma: To collect plasma, use EDTA, heparin, or citrate as an anticoagulant. Within 30 minutes of whole blood collection, centrifuge for 15 minutes at 1000 x g and remove plasma immediately. Sample Storage: Store assay samples immediately after collection or aliquot and store them at or below -20 C for up to 1 year. Avoid repeated freezethaw of samples. We have demonstrated sample stability up to five freezethaw cycles; we strongly recommend that each lab generate their own stability data. Avoid using samples with gross hemolysis or lipemia. ASSAY PROCEDURE Step 1 Add 100 µl of prepared coating solution to the plate. Incubate for approx. 1 hour at RT, shaking at approx. 300 rpm. Step 2 Discard plate contents and tap dry on paper towel. Add 200 µl blocking buffer to the plate. Incubate for approx. 1 hour at RT, shaking at approx. 300 rpm. Step 3 Step 4 Step 5 Step 6 Discard plate contents and wash 3x with 300 μl of wash buffer per well. Tap dry on paper towel. Dilute prepared controls and test samples 1/100 with assay buffer (e.g. 5 μl control/sample to 495 μl buffer, total volume 500 μl). Mix well. Do not store diluted controls or test samples. Add 100 µl of diluted controls/samples to the plate. Incubate for approx. 1 hour at RT, shaking at approx. 300 rpm. Discard plate contents and wash 3x with 300 μl of wash buffer per well. Tap dry on paper towel. 6

7 Step 7 Step 8 Step 9 Add 100 µl of prepared detection reagent to the plate. Incubate for approx. 1 hour at RT, shaking at approx. 300 rpm. Discard plate contents and wash 3x with 300 μl of wash buffer per well. Tap dry on paper towel. Add 100 µl of TMB to the plate. Incubate for approx minutes at RT protected from light. Step 10 Add 100 µl of TMB stop solution to the plate. Mix by gently tapping the side of the plate. Step 11 Determine absorbance with microplate reader at 450 nm with background correction at 620 nm. KEY STEPS Add coating solution Incubate 1 hour Tap plate dry Add blocking buffer Incubate 1 hour Wash plate Dilute controls and test samples 1/100 Add controls and samples Incubate 1 hour Wash plate Add detection reagent Incubate 1 hour Wash plate Add TMB Incubate 5-10 minutes Add TMB stop solution Read plate at 450 nm with background correction at 620 nm 7

8 CALCULATIONS & RESULTS 1. The results are reported as positive or negative relative to a predetermined cut-point. 2. The cut-point may be determined on each plate by running 3-6 negative controls (naïve individual human samples) and calculating their mean, then adding 2* standard deviation to this calculated mean. 3. An alternative cut-point may be calculated to fit the purpose of the study as described in G. Shankar, et al. (2008). QUALITY CONTROLS Good Laboratory Practice (GLP) requires both positive and negative quality control (QC) samples be included in each run to check the assay performance. Each laboratory should prepare and assay these QCs repeatedly (at least 3 times) to establish mean values and acceptable ranges. Each individual laboratory is responsible for defining their system for quality control decisions and is also responsible for making this system a written part of their laboratory procedures. It is strongly recommended to use 4-6-x criteria to accept or reject a run based on FDA Bioanalytical Guidance. 8

9 REFERENCES 1. Bolli, G. et. al. (1999). "Insulin analogues and their potential in the management of diabetes mellitus.". Diabetologia 42 (10): Kaplan, W.; et al. (2004). "Effects of Mixing Insulin aspart and Short- Acting Insulin Analogs on Glucose Control". Diabetes Care 27 (11): doi: /diacare PMID EPAR Lantus, German summary of admission report of EMEA (PDF) 4. L. Raymond Reynolds, MD, FACP, FACE, ECNU (January 2010). "Comparing Insulins Detemir and Insulin aspart in Type 2 Diabetes: More Similarities than Differences". Postgraduate Medicine 122 (1): European Medicines Agency update on safety of insulin Insulin aspart, July 23, G. Shankar, et al. (2008). Recommendations for the Validation of Immunoassays Used for Detection of Host Antibodies Against Biotechnology Products. J. Pharmaceutical and Biomedical Analysis 48: Creative Diagnostics. All rights reserved Ramsey Road Shirley, NY 11967, USA Tel: Fax:

Exendin-4 (Exenatide) ELISA Kit

Exendin-4 (Exenatide) ELISA Kit Exendin-4 (Exenatide) ELISA Kit Catalog: DEIABL227 For the quantitative determination of Exendin-4 in serum or plasma using competitive ELISA method For Research Use Only. Protocol Provided for Informational

More information

Mouse C-Peptide ELISA Kit

Mouse C-Peptide ELISA Kit Mouse C-Peptide ELISA Kit Cat.No: DEIA4507 Lot. No. (See product label) Size 96T Intended Use The Mouse C-Peptide ELISA kit is for the quantitative determination of c-peptide in mouse serum, plasma, and

More information

Human HIV (1+2) antigen&antibody ELISA Kit

Human HIV (1+2) antigen&antibody ELISA Kit Human HIV (1+2) antigen&antibody ELISA Kit Catalog Number. CSB-E18042h For the qualitative determination of human HIV (1+2) antibody and P24 antigen concentrations in serum, plasma. This package insert

More information

Morinaga Mouse C-peptide ELISA Kit

Morinaga Mouse C-peptide ELISA Kit Morinaga Mouse C-peptide ELISA Kit For the quantitative determination of C-peptide in mouse serum, plasma, and fluid 96wells For Laboratory Use Only, not for use in diagnostic procedure Please read full

More information

HbA1c (Human) ELISA Kit

HbA1c (Human) ELISA Kit HbA1c (Human) ELISA Kit Cat. No.:DEIA3509 Pkg.Size:96T Intended use GHbA1c (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human GHbA1c. General Description vhemoglobin,

More information

Mouse Leptin ELISA Kit Instructions

Mouse Leptin ELISA Kit Instructions V.6/Mar/2008 CRYSTAL CHEM INC. Mouse Leptin ELISA Kit Instructions For the quantitative determination of leptin in mouse serum or plasma and fluid Catalog #: 90030 96 Assays For Research Use Only. Not

More information

Rat Insulin ELISA. For the quantitative determination of insulin in rat serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures.

Rat Insulin ELISA. For the quantitative determination of insulin in rat serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures. Rat Insulin ELISA For the quantitative determination of insulin in rat serum and plasma For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: Size: 80-INSRT-E01, E10 96 wells, 10

More information

Human HBcAb IgM ELISA kit

Human HBcAb IgM ELISA kit Human HBcAb IgM ELISA kit Catalog number: NR-R10163 (96 wells) The kit is designed to qualitatively detect HBcAb IgM in human serum or plasma. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PURPOSES

More information

LDL (Human) ELISA Kit

LDL (Human) ELISA Kit LDL (Human) ELISA Kit Cat. No.:DEIA3864 Pkg.Size:96T Intended use This immunoassay kit allows for the specific measurement of human low density lipoprotein, LDL concentrations in cell culture supernates,

More information

Mouse Ultrasensitive Insulin ELISA

Mouse Ultrasensitive Insulin ELISA Mouse Ultrasensitive Insulin ELISA For the quantitative determination of insulin in mouse serum and plasma. Please read carefully due to Critical Changes, e.g., Calculation of Results. For Research Use

More information

Rat cholesterol ELISA Kit

Rat cholesterol ELISA Kit Rat cholesterol ELISA Kit Catalog No. CSB-E11706r (96T) This immunoassay kit allows for the in vitro quantitative determination of rat Cholesterol concentrations in serum, plasma and other biological fluids.

More information

Insulin ELISA. For the quantitative determination of insulin in serum and plasma.

Insulin ELISA. For the quantitative determination of insulin in serum and plasma. Insulin ELISA For the quantitative determination of insulin in serum and plasma. For In Vitro Diagnostic use within the United States of America. This product is for Research Use Only outside of the United

More information

Human Thyroid-Peroxidase Antibody, TPO-Ab ELISA Kit

Human Thyroid-Peroxidase Antibody, TPO-Ab ELISA Kit Human Thyroid-Peroxidase Antibody, TPO-Ab ELISA Kit Catalog No: E0442h 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH

More information

Insulin (Porcine/Canine) ELISA

Insulin (Porcine/Canine) ELISA Insulin (Porcine/Canine) ELISA For the quantitative measurement of insulin in Porcine/Canine serum and plasma (EDTA) For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 80-INSPO-E01

More information

IV2-113E Use by. Invitron Glargine ELISA Kit REF LOT IVD. Definitions. English. For in-vitro diagnostic use. Instructions for use.

IV2-113E Use by. Invitron Glargine ELISA Kit REF LOT IVD. Definitions. English. For in-vitro diagnostic use. Instructions for use. Definitions Instructions for use REF Catalogue number IV2-113E Use by English Invitron Glargine ELISA Kit For in-vitro diagnostic use Σ 96 LOT IVD Lot/Batch Code Storage temperature limitations In vitro

More information

STAT3 (py705)/ Pan STAT3 (Human/Mouse/Rat) ELISA Kit

STAT3 (py705)/ Pan STAT3 (Human/Mouse/Rat) ELISA Kit STAT3 (py705)/ Pan STAT3 (Human/Mouse/Rat) ELISA Kit Catalog Number KA2176 96 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Principle of the Assay...

More information

Mouse C-peptide ELISA

Mouse C-peptide ELISA Mouse C-peptide ELISA For the quantitative determination of C-peptide in mouse serum. For Research Use Only. Not for use in Diagnostic Procedures. Please read carefully due to Critical Changes, e.g., Calculation

More information

Procine sphingomyelin ELISA Kit

Procine sphingomyelin ELISA Kit Procine sphingomyelin ELISA Kit For the quantitative in vitro determination of Procine sphingomyelin concentrations in serum - plasma - celiac fluid - tissue homogenate - body fluid FOR LABORATORY RESEARCH

More information

EliKine Free Thyroxine (ft4) ELISA Kit

EliKine Free Thyroxine (ft4) ELISA Kit EliKine Free Thyroxine (ft4) ELISA Kit Booklet Item NO. KET0005 Product Name EliKine Free Thyroxine (ft4) ELISA Kit ATTENTION For laboratory research use only. Not for clinical or diagnostic use TABLE

More information

Rat Proinsulin ELISA

Rat Proinsulin ELISA Rat Proinsulin ELISA For the quantitative determination of proinsulin in rat serum For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 80-PINRT-E01 Size: 96 wells Version: June

More information

Bovine Insulin ELISA

Bovine Insulin ELISA Bovine Insulin ELISA For quantitative determination of insulin in bovine serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 80-INSBO-E01 Size: 96 wells Version:

More information

Rat C-peptide ELISA. For the quantitative determination of C-peptide in rat serum

Rat C-peptide ELISA. For the quantitative determination of C-peptide in rat serum Rat C-peptide ELISA For the quantitative determination of C-peptide in rat serum Please read carefully due to Critical Changes, e.g., see Calculation of Results. For Research Use Only. Not For Use In Diagnostic

More information

Porcine/Canine Insulin ELISA

Porcine/Canine Insulin ELISA Porcine/Canine Insulin ELISA For the quantitative determination of insulin in porcine or canine serum and plasma. Please read carefully due to Critical Changes, e.g., Calculation of Results. For Research

More information

Mouse C-peptide ELISA

Mouse C-peptide ELISA Mouse C-peptide ELISA For the quantitative determination of C-peptide in mouse serum. For Research Use Only. Not for use in Diagnostic Procedures. Please read carefully due to Critical Changes, e.g., Preparation

More information

Canine Thyroid Stimulating Hormone, TSH ELISA Kit

Canine Thyroid Stimulating Hormone, TSH ELISA Kit Canine Thyroid Stimulating Hormone, TSH ELISA Kit Catalog No: E0463c 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH

More information

STAT1 (ps727) (Human/Mouse) ELISA Kit

STAT1 (ps727) (Human/Mouse) ELISA Kit STAT1 (ps727) (Human/Mouse) ELISA Kit Catalog Number KA2171 96 assays Version: 01 Intended for research use only www.abnova.com I. INTRODUCTION STAT1 (ps727) (Human/Mouse) ELISA (Enzyme-Linked Immunosorbent

More information

Rat Mullerian Inhibiting Substance/Anti-Mullerian hormone, MIS/AMH ELISA kit

Rat Mullerian Inhibiting Substance/Anti-Mullerian hormone, MIS/AMH ELISA kit Rat Mullerian Inhibiting Substance/Anti-Mullerian hormone, MIS/AMH ELISA kit Catalog No. E0228r (96 tests) Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!

More information

Mouse HBsAg(Hepatitis B Virus Surface Antigen) ELISA Kit

Mouse HBsAg(Hepatitis B Virus Surface Antigen) ELISA Kit Mouse HBsAg(Hepatitis B Virus Surface Antigen) ELISA Kit Catalogue No.: EM0002 Size: 96T Reactivity: Mouse Application: This immunoassay kit allows for the qualitative determination of HBsAg in Mouse serum

More information

STAT3 (py705) (Human/Mouse/Rat) ELISA Kit

STAT3 (py705) (Human/Mouse/Rat) ELISA Kit STAT3 (py705) (Human/Mouse/Rat) ELISA Kit Catalog Number KA2175 96 assays Version: 01 Intended for research use only www.abnova.com I. INTRODUCTION STAT3 (py705) (Human/Mouse/Rat) ELISA (Enzyme-Linked

More information

Rat C-peptide ELISA. For the quantitative determination of C-peptide in rat serum. For Research Use Only. Not For Use In Diagnostic Procedures.

Rat C-peptide ELISA. For the quantitative determination of C-peptide in rat serum. For Research Use Only. Not For Use In Diagnostic Procedures. Rat C-peptide ELISA For the quantitative determination of C-peptide in rat serum. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: Size: 80-CPTRT-E01 96 wells Version: May 26,

More information

Serum Amyloid A ELISA

Serum Amyloid A ELISA Serum Amyloid A ELISA For the quantitative determination of serum amyloid A (SAA) in serum plasma For Research use Only. Not for Use in Diagnostic Procedures Please see Appendix A for Reference Serum information

More information

Insulin ELISA. For the quantitative determination of insulin in serum and plasma

Insulin ELISA. For the quantitative determination of insulin in serum and plasma Insulin ELISA For the quantitative determination of insulin in serum and plasma For In Vitro Diagnostic use within the United States of America. This product is for Research Use Only outside of the United

More information

Human TSH ELISA Kit. User Manual

Human TSH ELISA Kit. User Manual Human TSH ELISA Kit User Manual Catalog number: GTX15585 GeneTex Table of Contents A. Product Description... 2 B. Kit Components... 3 C. Additional Required Materials (not included)... 3 D. Reagent Preparation...

More information

HBeAg and HBeAg Ab ELISA Kit

HBeAg and HBeAg Ab ELISA Kit HBeAg and HBeAg Ab ELISA Kit Catalog Number KA0290 96 assays Version: 17 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3

More information

Morinaga Mouse/Rat Leptin ELISA Kit

Morinaga Mouse/Rat Leptin ELISA Kit V.1/Oct/2012 Morinaga Mouse/Rat Leptin ELISA Kit For the quantitative determination of leptin in mouse/rat serum, plasma, and fluid. 96 wells For Laboratory Use Only, not for use in diagnostic procedure

More information

Human Neuropeptide Y (NPY) ELISA KIT

Human Neuropeptide Y (NPY) ELISA KIT Human Neuropeptide Y (NPY) ELISA KIT Cat. No.:DEIA7404 Pkg.Size:96T Intended use The Human Neuropeptide Y (NPY) ELISA KIT is used for the non-radioactive quantification of Neuropeptide Y (NPY) in human

More information

RayBio Human Phospho-DDR1 (Tyr792) ELISA Kit

RayBio Human Phospho-DDR1 (Tyr792) ELISA Kit RayBio Human Phospho-DDR1 (Tyr792) ELISA Kit Catalog #: PEL-DDR1-Y792 User Manual Last revised March 22, 2018 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane,

More information

RayBio Human Phospho-DDR2 (Tyr740) and Total DDR2 ELISA Kit

RayBio Human Phospho-DDR2 (Tyr740) and Total DDR2 ELISA Kit RayBio Human Phospho-DDR2 (Tyr740) and Total DDR2 ELISA Kit Catalog #: PEL-DDR2-Y740-T User Manual Last revised March 22, 2018 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607

More information

Apolipoprotein A-1 ELISA

Apolipoprotein A-1 ELISA Apolipoprotein A-1 ELISA For the quantitative determination of apolipoprotein A1 in serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures. Please read carefully due to Critical

More information

RayBio Human Phospho-DDR1 (Tyr792) and Total DDR1 ELISA Kit

RayBio Human Phospho-DDR1 (Tyr792) and Total DDR1 ELISA Kit RayBio Human Phospho-DDR1 (Tyr792) and Total DDR1 ELISA Kit Catalog #: PEL-DDR1-Y792-T User Manual Last revised March 22, 2018 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607

More information

EGFR (py1045)/ Pan EGFR (Human) ELISA Kit

EGFR (py1045)/ Pan EGFR (Human) ELISA Kit EGFR (py1045)/ Pan EGFR (Human) ELISA Kit Catalog Number KA2156 96 assays Version: 01 Intended for research use only www.abnova.com I. INTRODUCTION EGFR (py1045)/pan EGFR (Human) ELISA (Enzyme-Linked Immunosorbent

More information

Fish follicle-stimulating hormone, FSH ELISA Kit

Fish follicle-stimulating hormone, FSH ELISA Kit Fish follicle-stimulating hormone, FSH ELISA Kit Catalog No: E0830f 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH

More information

Ultra-sensitive Human TSH ELISA Kit. MyBioSource.com

Ultra-sensitive Human TSH ELISA Kit. MyBioSource.com Ultra-sensitive Human TSH ELISA Kit For the quantitative determination of human thyroid stimulating hormone (TSH) concentrations in serum Catalog Number: MBS590037 96 tests FOR LABORATORY RESEARCH USE

More information

RayBio Human, Mouse and Rat Phospho-NF-kB P65 (Ser536) and Total NF-kB P65 ELISA Kit

RayBio Human, Mouse and Rat Phospho-NF-kB P65 (Ser536) and Total NF-kB P65 ELISA Kit RayBio Human, Mouse and Rat Phospho-NF-kB P65 (Ser536) and Total NF-kB P65 ELISA Kit Catalog #: PEL-NFKBP65-S536-T User Manual Last revised October 10, 2017 Caution: Extraordinarily useful information

More information

Epinephrine/Norepinephrine ELISA Kit

Epinephrine/Norepinephrine ELISA Kit Epinephrine/Norepinephrine ELISA Kit Cat. No.:DEIA3368 Pkg.Size:96T Intended use Enzyme Immunoassay for the quantitative determination of Adrenaline (Epinephrine) and Noradrenaline Norepinephrine) in plasma

More information

RayBio Human Phosphotyrosine BTK ELISA Kit

RayBio Human Phosphotyrosine BTK ELISA Kit RayBio Human Phosphotyrosine BTK ELISA Kit Catalog #: PEL-BTK-Y User Manual Last revised August 10, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite

More information

Hepatitis A virus IgM ELISA Kit

Hepatitis A virus IgM ELISA Kit Hepatitis A virus IgM ELISA Kit Catalog Number KA0285 96 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle

More information

Canine Insulin ELISA Kit

Canine Insulin ELISA Kit Canine Insulin ELISA Kit Cat. No.:DEIA2033 Pkg.Size:96T Intended use This product provides a method for the quantitative determination of insulin in canine serum and plasma. General Description Insulin

More information

TECHNICAL BULLETIN. Catalog Number RAB0447 Storage Temperature 20 C

TECHNICAL BULLETIN. Catalog Number RAB0447 Storage Temperature 20 C Phospho-Stat3 (ptyr 705 ) and pan-stat3 ELISA Kit for detection of human, mouse, or rat phospho-stat3 (ptyr 705 ) and pan-stat3 in cell and tissue lysates Catalog Number RAB0447 Storage Temperature 20

More information

FinTest TM IgG4 Screen 88 ELISA Kit

FinTest TM IgG4 Screen 88 ELISA Kit FinTest TM IgG4 Screen 88 ELISA Kit Cat. No.:DEIA6227 Pkg.Size:96T Intended use The Kit is for the quantitative determination of IgG4 antibodies against 88 Food Allergens in human serum, plasma and capillary

More information

RayBio Human Granzyme B ELISA Kit

RayBio Human Granzyme B ELISA Kit RayBio Human Granzyme B ELISA Kit Catalog #: ELH-GZMB User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,

More information

INTACT HUMAN PROINSULIN ELISA KIT 96-Well Plate (Cat. # EZHIPI-17K )

INTACT HUMAN PROINSULIN ELISA KIT 96-Well Plate (Cat. # EZHIPI-17K ) INTACT HUMAN PROINSULIN ELISA KIT 96-Well Plate (Cat. # EZHIPI-17K ) I. Intended Use 2 II. Principles Of Procedure 2 III. Reagents Supplied 2 IV. Storage and Stability 4 V. Reagent Precautions 4 VI. Materials

More information

TECHNICAL BULLETIN. Phospho-Akt (pser 473 ) ELISA Kit for detection of human, mouse, or rat phospho-akt (pser 473 ) in cell and tissue lysates

TECHNICAL BULLETIN. Phospho-Akt (pser 473 ) ELISA Kit for detection of human, mouse, or rat phospho-akt (pser 473 ) in cell and tissue lysates Phospho-Akt (pser 473 ) ELISA Kit for detection of human, mouse, or rat phospho-akt (pser 473 ) in cell and tissue lysates Catalog Number RAB0011 Storage Temperature 20 C TECHNICAL BULLETIN Product Description

More information

Influenza A IgG ELISA

Influenza A IgG ELISA Influenza A IgG ELISA For the qualitative determination of IgG-class antibodies against Influenza A virus in human serum or plasma (citrate, heparin). For Research Use Only. Not For Use In Diagnostic Procedures.

More information

Human Alpha 1 microglobulin ELISA Kit

Human Alpha 1 microglobulin ELISA Kit Human Alpha 1 microglobulin ELISA Kit Catalogue No.: EH4144 Size: 48T/96T Reactivity: Human Range:0.625-40ng/ml Sensitivity:

More information

SIV p27 Antigen ELISA Catalog Number:

SIV p27 Antigen ELISA Catalog Number: INTENDED USE The RETRO-TEK SIV p27 Antigen ELISA is for research use only and is not intended for in vitro diagnostic use. The RETRO-TEK SIV p27 Antigen ELISA is an enzyme linked immunoassay used to detect

More information

25(OH) Vitamin D ELISA (BD-220BA), 192 Tests

25(OH) Vitamin D ELISA (BD-220BA), 192 Tests INTENDED USE The 25-hydroxy (25-OH) Vitamin D ELISA is intended for the quantitative determination of total 25-OH Vitamin D in human serum and Plasma. SUMMARY AND EXPLANATION Vitamin D is a steroid hormone

More information

HIV-1 p24 Antigen ELISA Catalog Number:

HIV-1 p24 Antigen ELISA Catalog Number: INTENDED USE The RETRO-TEK HIV-1 p24 Antigen ELISA is supplied for research purposes only. It is not intended for use in the diagnosis or prognosis of disease, or for screening and may not be used as a

More information

Rat Leptin 96-Well Plate Assay Cat. # EZRL-83K

Rat Leptin 96-Well Plate Assay Cat. # EZRL-83K Rat Leptin 96-Well Plate Assay Cat. # EZRL-83K RAT LEPTIN ELISA KIT 96-Well Plate (Cat. # EZRL-83K) I. Intended Use 2 II. Principles Of Procedure 2 III. Reagents Supplied 2 IV. Storage and Stability 4

More information

Mouse C3 (Complement Factor 3) ELISA Kit

Mouse C3 (Complement Factor 3) ELISA Kit Mouse C3 (Complement Factor 3) ELISA Kit Cat. No.:DEIA8289 Pkg.Size:96T Intended use The Mouse C3 (Complement Factor 3) ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring

More information

Vedolizumab Drug Level ELISA

Vedolizumab Drug Level ELISA Vedolizumab Drug Level ELISA For the quantitative determination of free Entyvio concentration in human serum and EDTA plasma. Please read carefully due to Critical Changes, e.g., Updates to specificity

More information

Mercodia Glucagon ELISA

Mercodia Glucagon ELISA Mercodia Glucagon ELISA Directions for Use 10-1271-01 REAGENTS FOR 96 DETERMINATIONS For Research Use Only Manufactured by Mercodia AB, Sylveniusgatan 8A, SE-754 50 Uppsala, Sweden EXPLANATION OF SYMBOLS

More information

Rabbit Glycated hemoglobin A1c (GHbA1c) ELISA Kit

Rabbit Glycated hemoglobin A1c (GHbA1c) ELISA Kit Rabbit Glycated hemoglobin A1c (GHbA1c) ELISA Kit Catalog No. CSB-E07011Rb (96 tests) This immunoassay kit allows for the in vitro quantitative determination of rabbit GHbA1c concentrations in cell culture

More information

CoQ10(Coenzyme Q10) ELISA Kit

CoQ10(Coenzyme Q10) ELISA Kit CoQ10(Coenzyme Q10) ELISA Kit Catalogue No.: EU0196 Size: 48T/96T Reactivity: Universal Detection Range: 0.781-50ng/ml Sensitivity:

More information

Thyroid Stimulating Hormone (S-TSH) Thyroid Stimulating

Thyroid Stimulating Hormone (S-TSH) Thyroid Stimulating ab108659 Thyroid Stimulating Hormone (S-TSH) Human ELISA Kit Instructions for Use For the quantitative measurement of Human Thyroid Stimulating Hormone (S-TSH) concentrations in serum. This product is

More information

CYTOMEGALOVIRUS (CMV) IgM ELISA Kit Protocol

CYTOMEGALOVIRUS (CMV) IgM ELISA Kit Protocol CYTOMEGALOVIRUS (CMV) IgM ELISA Kit Protocol (Cat. No.:EK-310-91) 330 Beach Road, Burlingame CA Tel: 650-558-8898 Fax: 650-558-1686 E-Mail: info@phoenixpeptide.com www.phoenixpeptide.com INTENDED USE The

More information

TNF-alpha ELISA. For Research Use Only. Not For Use In Diagnostic Procedures.

TNF-alpha ELISA. For Research Use Only. Not For Use In Diagnostic Procedures. TNF-alpha ELISA For the quantitative determination of TNF-alpha in serum, plasma, buffered solution or cell culture medium. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number:

More information

Human Thyroglobulin EIA

Human Thyroglobulin EIA Human Thyroglobulin EIA For the quantitative determination of human thyroglobulin (htg) in plasma and serum. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 30-7510 Size: 96

More information

Human IL-2 ELISA Antibody Pair

Human IL-2 ELISA Antibody Pair Human IL-2 ELISA Antibody Pair For the qualitative and quantitative determination of IL-2 in serum, plasma and cell culture. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number:

More information

Instructions for Use. IA2 Antibody ELISA. Enzyme Immuno Assay for the Quantitative Determination of Antibodies against IA-2 in Serum.

Instructions for Use. IA2 Antibody ELISA. Enzyme Immuno Assay for the Quantitative Determination of Antibodies against IA-2 in Serum. Instructions for Use IA2 Antibody ELISA Enzyme Immuno Assay for the Quantitative Determination of Antibodies against IA-2 in Serum I V D REF EA114/96 12 x 8 2 8 C DLD Gesellschaft für Diagnostika und medizinische

More information

Toxoplasma gondii IgM ELISA Kit

Toxoplasma gondii IgM ELISA Kit Toxoplasma gondii IgM ELISA Kit Catalog Number KA0226 96 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle

More information

Human Apolipoprotein A1 EIA Kit

Human Apolipoprotein A1 EIA Kit A helping hand for your research Product Manual Human Apolipoprotein A1 EIA Kit Catalog Number: 83901 96 assays 1 Table of Content Product Description 3 Assay Principle 3 Kit Components 3 Storage 4 Reagent

More information

NF-κB p65 (Phospho-Thr254)

NF-κB p65 (Phospho-Thr254) Assay Biotechnology Company www.assaybiotech.com Tel: 1-877-883-7988 Fax: 1-877-610-9758 NF-κB p65 (Phospho-Thr254) Colorimetric Cell-Based ELISA Kit Catalog #: OKAG02015 Please read the provided manual

More information

Toxoplasma gondii IgM ELISA Kit

Toxoplasma gondii IgM ELISA Kit Toxoplasma gondii IgM ELISA Kit Catalog Number KA0226 96 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle

More information

ACTG Laboratory Technologist Committee Revised Version 2.0 ACTG Lab Man Coulter HIV-1 p24 ELISA May 21, 2004

ACTG Laboratory Technologist Committee Revised Version 2.0 ACTG Lab Man Coulter HIV-1 p24 ELISA May 21, 2004 Coulter HIV p24 1. PRINCIPLE The Human Immunodeficiency Virus Type 1 (HIV-1) is recognized as the etiologic agent of acquired immunodeficiency syndrome (AIDS). The virus is transmitted by sexual contact,

More information

FinTest IgG4 Screen 20 ELISA KIT

FinTest IgG4 Screen 20 ELISA KIT FinTest IgG4 Screen 20 ELISA KIT Cat. No.:DEIA6196 Pkg.Size:96T Intended use Enzyme immunoassay (microtiter strips) for the detection and the quantitative determination of IgG4 antibodies against 20 Food

More information

Human Mullerian Inhibiting Substance/Anti-Mullerian hormone, MIS/AMH ELISA Kit

Human Mullerian Inhibiting Substance/Anti-Mullerian hormone, MIS/AMH ELISA Kit Human Mullerian Inhibiting Substance/Anti-Mullerian hormone, MIS/AMH ELISA Kit Catalog No: E0228h 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!

More information

This kit is intended for Research Use Only. Not for use in diagnostic procedures.

This kit is intended for Research Use Only. Not for use in diagnostic procedures. This kit is intended for Research Use Only. Not for use in diagnostic procedures. Introduction The DRG Epstein-Barr Virus (VCA) IgM Enzyme Immunoassay Kit provides materials for determination of IgM-class

More information

For the quantitative determination of Glucagon concentrations in cell culture supernates, serum and plasma.

For the quantitative determination of Glucagon concentrations in cell culture supernates, serum and plasma. Glucagon ELISA Kit Cat.No: DEIA2827 Lot. No. (See product label) Size 96T Intended Use For the quantitative determination of Glucagon concentrations in cell culture supernates, serum and plasma. General

More information

Instructions for Use. Tg Antibody ELISA

Instructions for Use. Tg Antibody ELISA Instructions for Use Tg Antibody ELISA Enzyme Immunoassay for the Quantitative Determination of Autoantibodies to Thyroglobulin (Tg) in Serum and Plasma I V D REF EA618/96 12 x 8 2 8 C DLD Gesellschaft

More information

Mercodia Equine Insulin ELISA

Mercodia Equine Insulin ELISA Mercodia Equine Insulin ELISA Directions for Use 10-1205-01 REAGENTS FOR 96 DETERMINATIONS Manufactured by Mercodia AB Sylveniusgatan 8A SE-754 50 Uppsala Sweden EXPLANATION OF SYMBOLS USED ON LABELS =

More information

Estrone EIA kit. For the quantitative determination of estrone in dried fecal extracts, urine and tissue culture media. Cat. No.

Estrone EIA kit. For the quantitative determination of estrone in dried fecal extracts, urine and tissue culture media. Cat. No. K-ASSAY KAMIYA BIOMEDICAL COMPANY KAMIYA BIOMEDICAL COMPANY Estrone EIA kit For the quantitative determination of estrone in dried fecal extracts, urine and tissue culture media Cat. No. KT-720 For Research

More information

Tryptophan ELISA Kit. Catalog Number KA assays Version: 06. Intended for research use only.

Tryptophan ELISA Kit. Catalog Number KA assays Version: 06. Intended for research use only. Tryptophan ELISA Kit Catalog Number KA1916 96 assays Version: 06 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...

More information

Mercodia Porcine C-peptide ELISA

Mercodia Porcine C-peptide ELISA Mercodia Porcine C-peptide ELISA Directions for Use 10-1256-01 REAGENTS FOR 96 DETERMINATIONS Manufactured by Mercodia AB Sylveniusgatan 8A SE-754 50 Uppsala Sweden INTENDED USE Mercodia Porcine C-peptide

More information

Human Cytomegalovirus IgM ELISA Kit

Human Cytomegalovirus IgM ELISA Kit Human Cytomegalovirus IgM Catalog No: IRAPKT2012 ELISA Kit Lot No: SAMPLE INTENDED USE The CMV IgM ELISA is intended for use in the detection of IgM antibodies to Cytomegalovirus (CMV) infection in human

More information

Chymotrypsin ELISA Kit

Chymotrypsin ELISA Kit Chymotrypsin ELISA Kit Cat. No.:DEIA10041 Pkg.Size:96T Intended use The Chymotrypsin ELISA Kit is a sandwich Enzyme Immuno Assay intended for the quantitative determination of Chymotrypsin in stool. General

More information

Amebiasis Ab E. histolytica IgG

Amebiasis Ab E. histolytica IgG Code: HP003 96 Test distribuito da: Amebiasis Ab E. histolytica IgG Amebiasis E. histolytica lgg test is an enzyme immunoassay for the detection of specific lgg antibodies against Anti - Entamoeba histolytica

More information

Human Immunodeficiency Virus type 1 (HIV-1) gp120 / Glycoprotein 120 ELISA Pair Set

Human Immunodeficiency Virus type 1 (HIV-1) gp120 / Glycoprotein 120 ELISA Pair Set Human Immunodeficiency Virus type 1 (HIV-1) gp120 / Glycoprotein 120 ELISA Pair Set Catalog Number : SEK11233 To achieve the best assay results, this manual must be read carefully before using this product

More information

Cotinine (Mouse/Rat) ELISA Kit

Cotinine (Mouse/Rat) ELISA Kit Cotinine (Mouse/Rat) ELISA Kit Catalog Number KA2264 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle

More information

Zinc Transporter 8 (ZnT8) Antibody ELISA

Zinc Transporter 8 (ZnT8) Antibody ELISA Instructions for Use Zinc Transporter 8 (ZnT8) Antibody ELISA Enzyme Immuno Assay for the Quantitative Determination of ZnT8 Autoantibodies in Serum I V D REF EA102/96 12 x 8 2 8 C DLD Gesellschaft für

More information

Human IL-2. Pre-Coated ELISA Kit

Human IL-2. Pre-Coated ELISA Kit Human IL-2 (Interleukin 2) Pre-Coated ELISA Kit Catalog No: 90-2083 1 96 well Format (96 tests) Detection Range: 31.2 2000 pg/ml Sensitivity: < 18.75 pg/ml This immunoassay kit allows for the in vitro

More information

Mouse Adiponectin Immunoassay Kit

Mouse Adiponectin Immunoassay Kit Antibody and Immunoassay Services Li Ka Shing Faculty of Medicine, The University of Hong Kong Mouse Adiponectin Immunoassay Kit Catalogue Number: 32010 For the quantitative determination of mouse adiponectin

More information

Human cholesterol, CH ELISA Kit

Human cholesterol, CH ELISA Kit Human cholesterol, CH ELISA Kit Catalog No: E1701h 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE

More information

Rat Leptin-HS ELISA FOR LABORATORY USE ONLY YANAIHARA INSTITUTE INC AWAKURA, FUJINOMIYA - SHI SHIZUOKA, JAPAN

Rat Leptin-HS ELISA FOR LABORATORY USE ONLY YANAIHARA INSTITUTE INC AWAKURA, FUJINOMIYA - SHI SHIZUOKA, JAPAN YK051 Rat Leptin-HS ELISA FOR LABORATORY USE ONLY YANAIHARA INSTITUTE INC. 2480-1 AWAKURA, FUJINOMIYA - SHI SHIZUOKA, JAPAN 418 0011 Contents Introduction 2 Characteristics 3 Composition 4 Method 5-6 Notes

More information

Human LDL Receptor / LDLR ELISA Pair Set

Human LDL Receptor / LDLR ELISA Pair Set Human LDL Receptor / LDLR ELISA Pair Set Catalog Number : SEK10231 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in

More information

Rubella virus IgG ELISA Kit

Rubella virus IgG ELISA Kit Rubella virus IgG ELISA Kit Catalog Number KA0223 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of

More information

GAD 65 Antibody ELISA

GAD 65 Antibody ELISA Instructions for Use GAD 65 Antibody ELISA Enzyme Immuno Assay for the Quantitative Determination of Antibodies against Glutamic Acid Decarboxylase in Serum I V D REF EA104/96 12 x 8 2 8 C DLD Gesellschaft

More information

Human LDL ELISA Kit. Innovative Research, Inc.

Human LDL ELISA Kit. Innovative Research, Inc. Human LDL ELISA Kit Catalog No: IRKTAH2582 Lot No: SAMPLE INTRODUCTION Human low-density lipoprotein (LDL) transports cholesterol from the liver to tissues where it is incorporated into cell membranes.

More information

ENA screen (Extractable Nuclear Antigen) ELISA

ENA screen (Extractable Nuclear Antigen) ELISA ENA screen (Extractable Nuclear Antigen) ELISA Catalog Number: ENA31-K01 Enzyme immunoassay for the determination of IgG antibodies to nuclear and cytoplasmic antigens in human serum and plasma. 20A Northwest

More information

Thyroxine (T4) Human ELISA Kit

Thyroxine (T4) Human ELISA Kit ab108662 Thyroxine (T4) Human ELISA Kit Instructions for Use For the quantitative measurement of Human Thyroxine (T4) concentrations in serum. This product is for research use only and is not intended

More information