Glucose Tolerance Test (Non Pregnant Adult)
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1 Pathology at the Royal Derby Glucose Tolerance Test (Non Hospital Pregnant Adult) Standard Clinical Guidelines Chemical Pathology Valid Until 31 st March 2015 Document Code: CHISCG10 Glucose Tolerance Test (Non Pregnant Adult) INTRODUCTION The fasting plasma glucose is the preferred test for the diagnosis of diabetes mellitus (DM). The oral glucose tolerance test (GTT) is used when analysis of fasting and/or random glucose levels have not provided a clear diagnosis. The diagnosis of DM should not be made on the basis of a single raised plasma glucose in the absence of symptoms. 75g of anhydrous glucose (or its equivalent) in a final volume of 300 ml is used as recommended by WHO. All glucose values in this document refer to venous plasma glucose levels. Glucose results obtained from near patient testing strips should not be used in the diagnosis of DM. INDICATIONS Diagnosis of DM. A GTT is indicated if: a) there is impaired fasting glycaemia, (fasting plasma glucose >6.0 mmol/l, but <7.0 mmol/l) b) previous history of gestational diabetes c) there is a possible low renal threshold for glucose i.e. glycosuria and random glucose <11.1 mmol/l. CONTRAINDICATIONS None SIDE EFFECTS Occasionally nausea, vomiting or diarrhoea as the glucose drink is hyperosmolar. PRECAUTIONS None Authorised by Julia Forsyth Page 1 of 5
2 PREPARATION Planning A GTT is an outpatient procedure carried out in the Phlebotomy Department at the LRCH or RDH. No appointment is necessary but the patient should come between and am. The test can also be carried out by appointment only at the phlebotomy clinics at Ripley Hospital, Heanor Hospital and the Belper Clinic of Babington Hospital. Some GP surgeries can also carry out the procedure. Patient The patient should have a normal diet containing more than 150g carbohydrate daily for three days prior to the test (see table below). Guide to the carbohydrate content of some common foods: Food item Approximate carbohydrate content (g) 1 Slice of bread 10 Tablespoon of rice 12 Egg sized potato 10 2 Weetabix 30 Packet of crisps 15 All medication the patient is receiving should be noted on the request form. Drugs such as oral contraceptives, steroids, thiazide and loop diuretics may impair glucose tolerance. The patient should fast from 10p.m. the previous evening, to give a minimum 10 hour fasting period, and not eat or drink anything other than water, until the test is completed. Equipment a) For use in GP surgeries Glucose - dissolve 1 packet of dextrose (82.5g glucose monohydrate) in 250 ml water, this gives a final volume of 300 ml. This is equivalent to 75g anhydrous glucose. The dextrose is lemon flavoured to make it more palatable. Chemical Pathology, RDH obtains the glucose from pharmacy and provides supplies for GPs. Note: Glucose BP is glucose monohydrate. b) For use in LRCH, RDH, Ripley, Babington and Heanor Hospitals Polycal - this is a carbohydrate drink based on maltodextrin, a partial hydrolysate of corn starch. It is supplied by Cow and Gate in 200 ml bottles. Only 113 ml is required for each patient. This is equivalent to 75g anhydrous glucose. If 3 patients are being tested 2 bottles are sufficient. Authorised by Julia Forsyth Page 2 of 5
3 Measure 113 ml Polycal into a special beaker, add water up to 200 ml mark. Secure plastic cap firmly onto beaker, shake to mix. Polycal is now ready. Note: A further 100 ml of water must be drunk by the patient to make the final volume 300 ml. c) For use if the patient has an allergy to lemon flavouring In exceptional circumstances, when a patient has an allergy to the lemon flavouring used in the glucose tablets, Lucozade may be used as an alternative. 410mL of Lucozade Energy Original (containing70kcal/100ml glucose) is equivalent to 75g anhydrous glucose. Please note that before May 2009 the formulation of Lucozade Energy Original contained 73kcal/100mL glucose and therefore only 394mL was required to deliver 75g glucose. It may be necessary to check the back of the packaging to confirm which formulation is in the bottle or can. d) Specimen tubes required Each test requires two fluoride/oxalate tubes. PROCEDURE The patient may drink additional water during the test and must be seated quietly throughout the test. Smoking is not permitted during the test. The GTT procedure is described below. Samples must be clearly labelled with patient name, date and time, e.g GTT procedure TIME Fasting sample BLOOD SAMPLE for glucose Minimum volume 1 ml fluoride/oxalate tube 0 hours Give glucose solution to be drunk within 5 minutes or Polycal drink, followed by 100 ml water, to be drunk within 5 minutes. 2 hours sample Minimum volume 1 ml fluoride/oxalate tube Authorised by Julia Forsyth Page 3 of 5
4 INTERPRETATION Normal Both of the following criteria must be met 1) Fasting glucose 6.0 mmol/l 2) 2 hour GTT glucose <7.8 mmol/l Diagnostic Criteria for Diabetes Mellitus Either of the following is diagnostic: 1) Classical symptoms of DM and any one of the following: a) fasting glucose > 7.0 b) random glucose > 11.1 mmol/l c) 2 hour GTT glucose > 11.1 mmol/l or 2) In an asymptomatic patient, any two of the following, obtained on separate occasions a) fasting glucose 7.0 mmol/l b) random glucose 11.1 mmol/l c) 2 hour GTT glucose 11.1 mmol/l Diagnostic Criteria for Impaired Glucose Tolerance Both of the following criteria must be met: 1) Fasting glucose <7.0 mmol/l and 2) 2 hour GTT glucose > 7.8 mmol/l but < 11.1 mmol/l Diagnostic Criteria for Impaired Fasting Glycaemia Both the following criteria must be met: 1) Fasting glucose >6.0 but <7.0 mmol/l 2) 2 hour GTT glucose <7.8 mmol/l Equivocal Results If the results of the GTT are equivocal the test should be repeated annually or sooner if the patient becomes symptomatic. A high index of suspicion should be maintained in a patient with equivocal results who also has a first degree relative with DM. Patients should not be falsely reassured that DM has been excluded when equivocal results are obtained. Authorised by Julia Forsyth Page 4 of 5
5 TURNAROUND TIME 24 hours REFERENCES AND FURTHER READING 1. American Diabetes Association Report of the expert committee on the diagnosis and classification of diabetes mellitus. Diabetes Care 1997; 20: WHO Expert Committee on Diabetes Mellitus. WHO Technical Report Series Recommendations for the Management of Diabetes. Diabetes UK October Reviewed by: Date: Valid Until: SCG group April 1995 May 2000 SCG group September 2001 October 2003 SCG group November 2003 October 2005 Amended J Forsyth December 2004 October 2005 SCG group March 2006 October 2007 Helen Seddon August 2008 October 2009 Helen Seddon December 2009 December 2011 R Stanworth/H Seddon March 2012 March 2015 Authorised by Julia Forsyth Page 5 of 5
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