ANALYTICAL METHOD DEVELOPMENT FOR SIMULTANEOUS ESTIMATION OF ATORVASTATIN CALCIUM AND SITAGLIPTIN PHOSPHATE BY RP- HPLC METHOD

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1 Page1548 Indo American Journal of Pharmaceutical Research, 2014 ISSN NO: Journal home page: INDO AMERICAN JOURNAL OF PHARMACEUTICAL RESEARCH ANALYTICAL METHOD DEVELOPMENT FOR SIMULTANEOUS ESTIMATION OF ATORVASTATIN CALCIUM AND SITAGLIPTIN PHOSPHATE BY RP- HPLC METHOD Gandhi Parikshit 1, Dhande Swati 1, Kadam Vilasrao 1 Bharati Vidyapeeth s College of Pharmacy, C.B.D. Belapur, Navi Mumbai. Maharashtra. ARTICLE INFO Article history Received 04/03/2014 Available online 30/03/2014 Keywords Atorvastatin Calcium, Sitagliptin Phosphate, RP-HPLC. ABSTRACT Analytical methods are made to establish the identity, purity, physical characteristics and potency of the drugs. Methods may also support safety and characterization studies or evaluations of drug performance. Atorvastatin calcium (ATV) and Sitagliptin phosphate (STG) are usually co-administered and as there was no analytical method developed so far a new reverse phase analytical high-performance liquid chromatographic (HPLC) method for the simultaneous estimation of ATV and STG was developed. The proposed method was based on the separation of two drugs in reversed phase mode with the help of HiQ Sil C-18 HS (4.6mm ø 250 nm) analytical column. Both the drugs were detected with the help of UV detector at the wavelength of 255 nm. The current method was validated according to the ICH guidelines for Linearity, Accuracy and Precision, System stability, Detection and Quantification and Percent recovery etc. Corresponding author Ms. Swati Dhande Department of Pharmacology, Bharati Vidyapeeth s College of Pharmacy. bvppharmacology@gmail.com Please cite this article in press as Ms. Swati Dhande et al. Analytical Method Development for Simultaneous Estimation of Atorvastatin Calcium and Sitagliptin Phosphate by RP- HPLC Method. Indo American Journal of Pharm Research.2014:4(03). Copy right 2014 This is an Open Access article distributed under the terms of the Indo American journal of Pharmaceutical Research, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

2 Page1549 INTRODUCTION Analytical methods are made to establish the identity, purity, physical characteristics and potency of the drugs. Methods may also support safety and characterization studies or evaluations of drug performance.sitagliptin phosphate (STG) is an oral antihypoglycemic drug which is highly selective dipeptidyl peptidase-4(dpp-4) inhibitor that prolongs the action of incretins. [1-3]. The metabolizing enzyme is CYP 3A4 and CYP 2CB. The plasma protein binding of these drug is 38%. Excretion occurs via fecal and urine route. Sitagliptin Phosphate (R)-4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-1-(2,4,5-trifluorophenyl)butan-2-amine Atorvastatin calcium (ATV) is a lipid-lowering agent. It is an inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG- CoA) reductase. The metabolising enzyme CYP 3A4. The plasma protein binding of atorvastatin is 95%. Excretion occurs via fecal, renal and urine route. Atorvastatin Calcium (3R,5R)-7-[2-(4-fluorophenyl)-3-phenyl-4-(phenylcarbamoyl)-5-propan-2-ylpyrrol-1-yl]-3,5-dihydroxyheptanoic acid These drug are used to treat the type II diabetes and hyperlipidemia respectively [4].These two drugs are usually co-administered and to the some extent have same pharmacokinetic parameters. As there was no analytical method for simultaneous estimation of these two drugs so far, an attempt was made to develop a simple and sensitive RP HPLC method for the simultaneous estimation of STG and ATV. MATERIAL AND METHODS Materials and Reagents: Working standards of pharmaceutical grade STG and ATV were obtained as generous gifts from Glenmark Generics, and from Rubicon Pharmaceuticals Pvt. Ltd. Mumbai respectively. Methanol, Acetonitrile of HPLC grade were purchased from SD Fine Chemicals, Mumbai. Method: Chromatographic system and conditions Jasco Manual HPLC System with a model no. HPLC PU 2080 PLUS was used for the separation of the compounds. The separation was being achieved on a HiQ sil C18HS (4.6mmø 250mm) analytical column. The method was optimised with the help of trial and error method. Various mobile phases were Methanol: Water 60: 40, 80: 20, 50-50, Acetonitrile : Water 70 :30, 60:35, 85:15, Ammonium acetate buffer (10mM, 15mM, 20mM. 25mM and 30 mm ) : Acetonitrile 45:55, 75:25, 50:50 etc. The mobile phase with optimum resolution and sharp peak was selected. The final mobile phase was acetonitrile: 20mM ammonium acetate buffer (ph adjusted to 4.5 using o-phosphoric acid with ph meter) in the ratio of 60:40 v/v. The flow rate was 0.8 ml/min and UV detection was performed at 255 nm. The mobile phase was shaken on an ultrasonic bath for 30 min. The resulting transparent mobile phase was filtered through a 0.45 μ membrane filter (Millipore, Ireland). A 20 μl sample was injected six times under optimized chromatographic conditions. The peak areas were measured at 255 nm.

3 Page1550 ABSORBANCE AREA Optimized chromatographic conditions Injection volume: 20µL Column: HiQsil C18HS (4.6mmø 250mm) Mobile phase: of Acetonitrile: 20mM ammonium acetate buffer (ph adjusted to 4.5 using o-phosphoric acid) (60:40, v/v) Flow rate: 0.8 ml/min UV detection: 255nm Run time: 10 minutes Preparation of standard stock solution 100mg of STG and ATV were weighed, transferred into 100 ml volumetric flasks and volume were made up to 100ml using methanol to get 1mg/ ml standard stock solutions respectively Preparation of working sample solution: 1ml from both of stock solution were pipetted out, transfer to 10ml volumetric flask and volume were made up to 10 ml with mobile phase to make a solutions of 100 ppm. Further for solution of 10 ppm again 1ml of above solution was pipetted out, transfer to 10ml volumetric flask and volume was made up to 10 ml with mobile phase. Chromatogram: ATV STG TIME (min) Figure. No. 1 - Chromatogram Method Validation: Validation of the optimized HPLC method was carried out according to ICH guidelines- Q2 (R1) [10] Linearity Working samples of 10 ppm solutions of ATV and STG were used for preparation of subsequent aliquots; aliquots of 2, 4, 6, 8, 10, and 20 ppm concentrations were prepared by serial dilution. 20 μl of each solution was being injected into column. All measurements were repeated three times for each concentration. The calibration curves of the area under curve versus concentration were recorded. Accuracy Marketed formulations (Tablets) ATEN 40 mg of Cadila Health Care (ATV) and JANUVIA 50 mg of Merck Sharp and Dohme (STG) were purchased and solutions of 10 ppm were prepared. 4.0, 5.0 and 6.0 ppm of ATV and 7.2, 8.0, and 8.8 ppm of these solutions were spiked in same concentrations of working samples of stock solution and were injected for the accuracy studies. The area under curve obtained was checked and analysed for the recovery percentage. Precision The precision of method was checked and verified by repeatability, inter-day and intra-day precision. Repeatability was checked by injecting 10 ppm concentration of ATV and STG for 6 times on the same day and for intraday precision 10 ppm

4 Page1551 concentration was injected and analysed at different time intervals on the same day. Similarly, for the interday precision 10 ppm concentration was analysed on different days. Limit of detection (LOD) and Limit of quantification (LOQ) The LOD and LOQ of ATV and STG were confirmed and recorded separately on the basis of signal (S) to noise (N) ratio. Robustness of the method To determine the robustness of the developed method, minute changes were made in the flow rate, percentage of organic phase, mobile phase ph and column compartment temperature. The deviations in all parameters from optimized method were studied. System suitability parameters To perform the system suitability test the standard solution were freshly prepared and injected under the condition of optimized method. RESULTS AND DISCUSSION A satisfactory separation was obtained when using Acetonitrile: 20 mm ammonium acetate buffer (ph = 4.5) in the ratio of 60:40 v/v under isocratic mode and a flow rate of 0.8 ml/min. Peaks were well defined, symmetrical and resolved. Retention time for STG and ATV were observed at 3.3 min and 7.1 min respectively and the optimum wavelength was determined to be 255 nm. Method was found to be linear in the range of 2-20 ppm for both drugs with regression coefficients of and for ATV and STG respectively. LINEARITY: Calibrations curve of STG and ATV over the concentration range 2-20 ppm were found to be linear and the (R 2 ) value was found to be and respectively Figure no. 2 Calibration Curve of STG

5 Page1552 Figure no. 3 - Calibration Curve of ATV LOD and LOQ: LOD and LOQ of STG and ATV were found to be 0.71 and 0.22 µg/ml, 2.1 and 0.69 µg/ml respectively. Drug % Level Atorvastatin Calcium Sitagliptin Phosphate Table no. 1 Results of Accuracy Concentration Total Amount after spiking Concentration Recovered (ppm) (ppm) (ppm) % Recovery ACCURACY: The accuracy is expressed as % recovery within the acceptable limits of all concentrations are given in table below PRECISION: A) Intraday: Table no. 2 Results of Intraday Precision Atorvastatin calcium Sitagliptin phosphate Level LQC MQC HQC LQC MQC HQC Amount (ppm) Peak Area (AU) Average Peak Area S.D %RSD

6 Page1553 B) Interday: SYSTEM SUITABILITY: Table no. 3 Results of Interday Precision Atorvastatin calcium Sitagliptin phosphate Level LQC MQC HQC LQC MQC HQC Amount (ppm) Peak Area DAY (AU) DAY DAY Average Peak Area S.D %RSD Table no. 4 Results of System Suitability Parameters Sitagliptin phosphate Atorvastatin calcium % RSD (Rt) % RSD (Peak Area) No. of theoretical plates Peak tailing Resolution ROBUSTNESS: Table no. 5 Result of Robustness %RSD Atorvastatin calcium Sitagliptin phosphate Parameter Deviation Area (AU) Retention Time Area Retention Time Flow rate (ml/min) % of Organic phase ph Column Compartment temperature ( o C) CONCLUSION This research work describe the new, simple and selective RP-HPLC method for Simultaneous determination of Atorvastatin calcium (ATV) and Sitagliptin phosphate (STG) and it was found to be precise and accurate. Thus, the above method can be used for bio-analytical method development and can also be applied for analysis of clinical samples. ACKNOWLEDGEMENT We would like to acknowledge Glenmark Generics and Rubicon Pvt. Ltd Mumbai for providing gift sample of Sitagliptin phosphate and Atorvastatin calcium respectively. We would like to thank Mr. Abhay Shirode for his valuable support. REFERENCES 1) Shailaja J, Swati K, Deepali D, Amol J, Praveen C, Development and Validation of RP-HPLC and HPTLC Methods for Simultaneous Estimation of Sitagliptin Phosphate and Metformin Hydrochloride in Bulk and Dosage form. Indian Journal of Pharmaceutical Education and Research.2013; 47(1): ) Samanthula G, Vaddepally L, Saladi S, Veeraraghavan S, Caringula G, Chennupati S, Rapid LC-ESI-MS-MS Method for the Simultaneous Determination of Sitagliptin and Pioglitazone in Rat Plasma and Its Application to Pharmacokinetic Study. American Journal of Analytical Chemistry, 2012, 3,

and Biomedical Analysis 41-2006 517 526. 4) Khan G, Sahu D, Agrawal Y P, Sabarwal N, Jain A, Gupta A K. Simultaneous Estimation of Metformin and Sitagliptin In Tablet Dosage Form.

7 Page1554 3) Barrett B., Huclova J, Borek-Dohalsky V, Nemeca B, Jelınek I, Validated HPLC MS/MS method for simultaneous determination of simvastatin and simvastatin hydroxy acid in human plasma, Journal of Pharmaceutical and Biomedical Analysis ) Khan G, Sahu D, Agrawal Y P, Sabarwal N, Jain A, Gupta A K. Simultaneous Estimation of Metformin and Sitagliptin In Tablet Dosage Form. Asian Journal of Biochemistry Pharmaceutical Research. 2011; 1(2): ) R Asmar, S Vol, B Pannier, A Brisac, J Tichet, A. El Hasnaoui, High blood-pressure and associated cardiovascular risk factors in France, Journal of Hypertension ; ) Shah VP. Workshop/Conference Report Best practices for chromatographic and ligand binding assay. Journal Pharmaceutical Research 2007; 9(1): ) Zeng W, Xu Y, Constanzer M, Woolf EJ. Determination of sitagliptinin human plasma using protein precipitation and tandem mass spectrometry. Journal of Chromatography. B. 2010; 878(21): ) Patil S, Ramesh B, Patil K, Dhokane A. Development and Validation of RP-HPLC Method for the Estimation of Sitagliptin Phosphate in Tablet Dosage Form. Asian Journal of Research Chemistry. 2010; 3(3): ) Shah Y, Iqbal Z, Ahmad L, Khan A, Khan M. I, Nazir S. and Nasir F Simultaneous determination of rosuvastatin and atorvastatin in human serum using RP-HPLC/UV detection: method development, validation and optimization of various experimental parameters. Journal of chromatography. 879, ) International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human use, ICH harmonised tripartite guideline, validation of analytical procedures: text and methodology Q2 (R1) Submit your next manuscript to IAJPR and take advantage of: Access Online first Double blind peer review policy No space constraints Rapid publication International recognition Submit your manuscript at: editorinchief@iajpr.com

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