The Effects of American Ginseng Extracts on Streptozocin Induced Diabetic Sciatic Neuropathy in Exercised Sprague Dawley Rats

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1 The Effects f American Ginseng Extracts n Streptzcin Induced Diabetic Sciatic Neurpathy in Exercised Sprague Dawley Rats By Guangyu Li Supervisr: Dr. Earl Nble

2 Agenda Backgrund Hypthesis Experimental design Methds and Materials Preliminary results Cnclusins

Backgrund Exercise and vascular well-being Endgenus antixidant defenses and heat shck prtein expressin Bld flw Shear stress Neur-activatin Ginseng and diabetes Expressin f endgenus antixidant enzymes

3 Backgrund Exercise and vascular well-being Endgenus antixidant defenses and heat shck prtein expressin Bld flw Shear stress Neur-activatin Ginseng and diabetes Expressin f endgenus antixidant enzymes (e.g. Cu/Zn superxide dismutase) Nitric xide (NO) synthesis Pr-inflammatry cytkines (e.g. TNF-α, IL-6, and C reactive prtein[crp]) DeBlieux,P.M.C., et al. (1993); Atalay, M, et al. (2003); De Angelis, K.L.D., et al (2000); Gillis, C.N.(1997)

4 Backgrund Diabetic Neurpathy (DN) 346 millin peple 50% Etilgy Frmatin f advanced glycatin end prducts Increased xygen free radical activity Reduced endthelial nitric xide activity Pathgenesis f DN Micrvascular abnrmalities Endthelial dysfunctin Reduced nerve bld flw Hypxia capillary damage escalating hypxia Ptential therapeutic appraches T increase nerve bld flw T prevent/reduce the generatin f advanced glycatin endprducts Treatment with antixidant Exercise&Ginseng Exercise&Ginseng Exercise&Ginseng Lw P.A. (1987); Tesfaye, S. et. Al (1994); Camern N.E. & Ctter M.A. (2007)

5 Hypthesis American ginseng aqueus extract (AQ-G) alng with physical exercise attenuates/prevents diabetic sciatic neurpathy by maintaining endneural vascular and nerve fiber integrity, and mdulating nitric xide pathway in STZ induced type-1 diabetic rats.

6 Experimental design Week 1 Week 2 Cntrl Diabetic Cntrl Diabetic Ginseng Diabetic Exercise Ginseng Diabetic Ginseng Recvery STZ injectin 20mg/kg (up t 5 injectins) Ginseng Ginseng&Exercise Week 5 Ginseng Week 12 Terminatin

7 Experimental design * * Eileen He (2011) Ginseng administratin American ginseng aqueus extract Supplied with drinking water 250mg/kg/day

8 Methds and Materials SD male Rats (8-week ld; g) Bld analysis HbA1c Lipid/lipprtein analysis Multiplex assay fr pr-inflammatry cytkine such as CRP, TNF-α, and IL-6 Ttal radical scavenging ability assay(trsa) Clrimetric reactin Reprted in mm Trlx equivalent Prtein immunblt/immunhistchemistry T examine endneural vasculature, and endthelial dysfunctin (mlecular markers within NO related pathway) Histchemistry H & E Luxl fast blue (myelin specific) Bielschwsky s silver stain (axn specific)

9 ug/ml mm Trlx Eq. Results Ginsenside cntent breakdwn ver 24 hurs Radical scavenging ability f aqueus ginseng extracts 140 Re 0.1 Rg American ginseng extracts. Rb Rc The amunt f detectable ginsenside 0.07 Rd decreases vertime The radical scavenging ability f aqueus 0.05 American ginseng extracts is Re, Rg1, Rb1, Rc, Rd are the majr ginsenside species fund in aqueus stabilized Hurs Hurs Ginsenside Time Re Rg1 Rb1 Rc Rd Ttal Remaining 0 Hr % 3 Hr % 6 Hr % 12 Hr % 24 Hr %

10 Results Glu. week 6 BW week 6 Glu. week12 BW week 12 Cntrl 4.0 ± ± ± ± 56.5 Diabetic Cntrl 18.9 ± 3.7* 350 ± 33* 20.9 ± 3.4* ± 49.9* Diabetes + Ginseng Mean with standard deviatin are reprted Cntrl (n=12); Diabetic cntrl (n=16); Diabetes + Ginseng (n=2) * Significant different p<0.05 HbA1C/Ttal Hb Triglyceride (mml/l) Cntrl 5.39 ± 1.20% 1.23 ± 0.43 Diabetic Cntrl 8.18 ± 1.47%* ± 8.54* Diabetes + Ginseng N/A 1.57 Mean with standard deviatin are reprted Cntrl (n=12); Diabetic cntrl (n=16); Diabetes + Ginseng (n=2) * Significant different p<0.05

11 Results Cntrl Diabetic Cntrl Diabetic + Ginseng H & E LFB 200x

12 Results Cntrl Diabetic Cntrl Diabetic + Ginseng H & E LFB 400x

13 Cnclusin S far.. The amunt f detectable ginsensides decreases vertime. Hwever, the ptency f radical scavenging ability f aqueus ginseng extracts is unaffected by time. T cnfirm diabetes, Plasma glucse level, bdy weight, endpint glycated hemglbin level, triglyceride level are all significantly different between Cntrl and Diabetic Cntrl grup. Frm the neural mrphlgy pint f view, Reductin f the myelinatin in diabetic cntrl grup Sign f endneural edema

14 Cnclusin We believe that the mdel we adpted is adequate t study diabetic peripheral neurpathy, and it has set up a reliable reference pint fr the future study.

16 Bdy weight (g) Bdy Weight * Week Cntrl Diabetic Cntrl Diabetic Recvery

17 Water cnsumpthin (ml) Water cnsumptin Day Diabetic Grup Cntrl Grup Diabetic Recvery

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