ARTICLE. Randomized Placebo-Controlled Trial of Metformin for Adolescents With Polycystic Ovary Syndrome

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1 ARTICLE Randomized Placebo-Controlled Trial of Metformin for Adolescents With Polycystic Ovary Syndrome Tracey Bridger, MD, FRCPC; Suzanne MacDonald, MD, FRCPC; Franziska Baltzer, MD, FRCPC; Celia Rodd, MD, FRCPC Objective: To determine whether metformin or placebo could, in conjunction with healthy lifestyle counseling, decrease serum testosterone levels and related aberrations in adolescents with hyperandrogenism, hyperinsulinemia, and polycystic ovarian syndrome. Design: Randomized, placebo-controlled, doubleblind trial. Setting: Pediatric university teaching hospital. Participants: Twenty-two adolescents aged 13 to 18 years with hyperinsulinemia and polycystic ovarian syndrome. Intervention: Participants were randomly assigned to take a 12-week course of either metformin or placebo. Main Outcome Measures: Pretreatment and posttreatment oral glucose tolerance tests, fasting lipid profiles, and clinical measurements. Results: There was a significant decline in mean serum testosterone concentration with metformin ( 38.3 ng/ dl) compared with placebo ( 0.86 ng/dl) (95% confidence interval, to 0.29 for the mean difference between groups). At completion, the relative risk of menses was 2.50 times higher in the metformin group compared with the placebo (95% confidence interval, 1.12 to 5.58). Measures of insulin sensitivity, including insulin area under the curve and HOMA (homeostasis model assessment), demonstrated improvement only with metformin, but these did not reach statistical significance. High-density lipoprotein cholesterol levels increased by 6.98 mg/dl with metformin vs a decrease of 2.33 mg/dl with placebo (95% confidence interval, 0.78 to for the mean difference between groups). There were no significant changes in body mass index, hirsutism, triglyceride levels, or total and low-density lipoprotein cholesterol levels. Conclusion: Metformin significantly lowered total testosterone concentrations, increased the likelihood of menses, and improved high-density lipoprotein cholesterol levels without affecting measures of insulin sensitivity or body weight. Arch Pediatr Adolesc Med. 2006;160: Author Affiliations: Department of Pediatrics, Memorial University of Newfoundland, St John s (Dr Bridger); Department of Pediatrics, McGill University, Montreal, Quebec (Drs MacDonald, Baltzer, and Rodd). POLYCYSTIC OVARY SYNDROME (PCOS) is a common disorder in women, characterized by menstrual dysfunction and evidence of hyperandrogenism (clinical or biochemical). 1,2 It is diagnosed in at least 5% to 10% of women between adolescence and menopause, making it one of the most prevalent hormonal disorders in this population. 3-6 Once thought to be purely a cosmetic and fertility problem, it is now known that women with PCOS have increased risk factors for cardiovascular disease and are at risk of developing diabetes. 7-9 Although the pathogenesis is not fully understood, evidence suggests insulin resistance and hyperinsulinemia play a key pathophysiologic role by promoting excess androgen production from both the ovaries and adrenal glands. 10,11 Many studies have demonstrated improvement of hyperinsulinemia, hyperandrogenemia, and clinical status in women by weight loss or treatment with insulinsensitizing agents (such as metformin or troglitazone). 12,13 Polycystic ovary syndrome is increasingly recognized in adolescent girls and is one of the most frequent causes of androgen excess in this age group. 14 The metabolic features seen in adult women with PCOS have also been reported in adolescents with the disorder, including hyperinsulinemia From these data, we infer that the pathogenesis is likely similar to that in adult women, which suggests a therapeutic role for reducing insulin levels or increasing insulin sensitivity in adolescents with PCOS. There are currently very few studies of metformin in adolescent girls, and its use in this population remains controversial. Moreover, there are no randomized, pla- 241

2 cebo-controlled studies of metformin in adolescents with PCOS. 1,14,18 The primary objective of this study was to determine whether metformin or placebo, in conjunction with healthy lifestyle counseling, could reduce serum testosterone levels in adolescents with hyperandrogenism, hyperinsulinemia, and PCOS. Secondary objectives centered on the efficacy of metformin in restoring menses, attenuating insulin resistance, and/or improving lipid profiles in adolescents with this disorder. METHODS STUDY PARTICIPANTS We enrolled 22 adolescents with PCOS and concomitant hyperinsulinemia from the Endocrinology and Adolescent Medicine clinics of the Montreal Children s Hospital (Montreal, Quebec) between 1999 and Participants were at least 12 years old and had a history of menarche at least 2 years prior to enrollment. Diagnosis of PCOS was based on previously reported criteria. 7 All subjects had chronic oligomenorrhea ( 6 menses in the preceding year) and clinical or biochemical evidence of hyperandrogenism. We excluded subjects with other causes of hyperandrogenism, such as hyperprolactinemia, androgen-secreting tumors, or late-onset congenital adrenal hyperplasia. Congenital adrenal hyperplasia was specifically ruled out by measuring 17 hydroxyprogesterone levels using an adrenocorticotropin (ACTH) stimulation test. Sample size was based on power calculations and the published effects of metformin treatment in adult women. We approached eligible candidates sequentially until this goal was achieved, and baseline demographic features did not differ between participants and nonparticipants (not shown). Exclusion criteria included diabetes mellitus, renal or hepatic disease, pregnancy, allergies to metformin, taking oral contraceptives in the 6 months prior to enrollment, or taking medications that could influence the effects of insulin or androgens. The study was approved by the research ethics board of the Montreal Children s Hospital. Each young woman gave informed consent and/or assent, and informed consent was obtained from their parents. STUDY PROTOCOL After a 12-hour overnight fast, the adolescents came to the study center and underwent a standard 75-g oral glucose tolerance test (OGTT). At baseline, serum was collected to measure total testosterone level, progesterone level, and lipid profile. At 0-, 30-, 60-, 90-, and 120-minute intervals after the glucose load, glucose and insulin levels were drawn. Glucose tolerance was assessed by criteria from the American Diabetes Association (Alexandria, Va). 19 Progesterone was measured to determine the phase of the menstrual cycle for accurate assessment of the serum lipids. We performed a complete physical examination for all participants, including measuring height and weight, determining blood pressure, and assessing hirsutism using the Ferriman and Gallwey scoring system. 20 This scoring system ranges from 0 to 36, with values greater or equal to 8 considered moderately hirsute. All participants were provided with healthy eating and physical activity counseling according to the Health Canada and Fitness Vitality healthy lifestyle program. A copy of this information was provided with a 3-month calendar to document medication adverse events, menses, and lifestyle changes. Patients were then randomized by draw sequential sampling, without replacement, to receive either metformin at the final dose of 750 mg or identically appearing placebo tablets twice per day. They were advised to increase the medications over a 4-week period to minimize adverse effects. Telephone contact was made after 4 to 6 weeks to discuss their progress. After 12 weeks, the adolescents returned for a second OGTT, baseline blood samples, and an interview as during the first visit. Unused pills were returned to estimate compliance. ASSAYS Blood samples were centrifuged immediately, and serum for hormones and high-density lipoprotein (HDL) cholesterol was stored at 20 C until assayed. Glucose, triglyceride, and total cholesterol levels were immediately analyzed on the Vitros (Ortho-Clinical, Johnson and Johnson, New Brunswick, NJ). Highdensity lipoprotein cholesterol was analyzed on the Cobas Miros platform (Roche Diagnostics, Hoffman La Roche Ltd, Basel, Switzerland). Progesterone was assayed by Centaur chemiluminescence platform (Chiron Corp, Emeryville, Calif). Insulin, dehydroepiandrosterone sulfate, and prolactin were analyzed by the chemiluminescent Immulite platform (Diagnostic Systems, Webster, Tex). Serum was analyzed by radio immunoassay kits for total testosterone (Pantax, Santa Monica, Calif), 17 beta estradiol, 17 hydroxyprogesterone (Diagnostic Systems) and luteinizing hormone and follicle-stimulating hormone (Diagnostic Products Corp, Los Angeles, Calif). Insulin sensitivity was estimated by calculating the insulin area under the curve (AUC), a measure that has been validated in women with PCOS against the hyperglycemiceuglycemic clamp. 21 We also calculated the homeostasis model assessment (HOMA=[fasting insulin result fasting glucose result]/22.5) and quantitative insulin sensitivity check index (QUICKI=1/[logarithm of fasting insulin result logarithm of fasting glucose result]), both previously validated using the hyperglycemic-euglycemic clamp DATA ANALYSIS Statistical analysis was performed with R, version For continuous variables, the difference between posttreatment and baseline values were calculated for each subject and the groups compared for equality of variance by F test. When variances were equal, the mean difference in each group and 95% confidence interval (CI) for the difference between the 2 means were tested for equality by a standard Welch t test procedure. The Satterthwaite method was invoked for unequal variances (null hypothesis, H O ; mean difference, 0). Categorical data (eg, return of menses) were compared by Fisher exact test and expressed as a relative risk with associated 95% confidence interval (H O relative risk, 1). In both cases, failure of the 95% confidence interval to cross the H O value reflects P.05. RESULTS Of 22 subjects recruited (n=11 in each group), only 1 subject did not complete the study. One adolescent in the placebo arm was able to provide information about her compliance and clinical well-being at the end of the study period but was unable to present for the OGTT and physical examination. PREINTERVENTION CHARACTERISTICS The clinical characteristics of the 2 groups of adolescent girls at baseline are depicted in Table 1. They ranged 242

3 Table 1. Clinical Characteristics and Serum Hormone Concentrations in Adolescents With PCOS at Baseline Characteristic Metformin Group Placebo Group Age, mean ± SD, y ± ± 1.39 Body mass index, mean ± SD* 33.6 ± ± 3.0 Ferriman and Gallwey score, mean ± SD 5.3 ± ± 7.6 Total testosterone, mean ± SD, ng/dl (nmol/l) ± 54.0 (6.03 ± 1.88) ± 46.0 (5.80 ± 1.58) Insulin AUC, mean ± SD, µu/ml min (pmol/l min) ± ( ± ) ± ( ± ) Fasting glucose, mean ± SD, mg/dl (mmol/l) 77.9 ± 7.2 (4.33 ± 0.4) 80.6 ± 8.3 (4.48 ± 0.46) HOMA, mean ± SD 5.75 ± ± 1.45 QUICKI, mean ± SD 0.34 ± ± 0.05 Total cholesterol, mean ± SD, mg/dl (mmol/l) ± 27.5 (4.25 ± 0.71) ± 48.9 (4.41 ± 1.26) LDL cholesterol, mean ± SD, mg/dl (mmol/l) 88.5 ± 19.8 (2.28 ± 0.51) 93.5 ± 41.9 (2.41 ± 1.08) HDL cholesterol, mean ± SD, mg/dl (mmol/l) 45.8 ± 18.6 (1.18 ± 0.48) 42.3 ± 10.9 (1.09 ± 0.28) Triglycerides, mean ± SD, mg/dl (mmol/l) ± 55.1 (1.53 ± 0.63) 168 ± 77.9 (1.92 ± 0.89) Ethnicity, No. White, non-hispanic 7 10 Black, non-hispanic 2 0 Hispanic 1 0 Asian 1 1 Menses in 6 months before enrollment, No. 2 3 Girls with menses during 6 months before enrollment, No. 2 3 Abbreviations: AUC, area under the curve; HDL, high-density lipoprotein; HOMA, homeostasis model assessment; LDL, low-density lipoprotein; PCOS, polycystic ovarian syndrome; QUICKI, quantitative insulin sensitivity check index. *Body mass index is calculated as weight in kilograms divided by the square of height in meters. in age from 13 to 18 years with an average age of 16.0 years at the start of the study period. All were overweight or obese with body mass index (BMI), calculated as weight in kilograms divided by the square of height in meters, ranging from 26.0 to By definition, all were hyperandrogenemic with significantly elevated levels of total testosterone. Subjects in both groups came from similar ethnic backgrounds. All participants had had fewer than 6 menses in the year preceding the study with no significant difference in the 2 proportions at baseline. POSTINTERVENTION CHARACTERISTICS At completion, the weights and BMI had not significantly changed. Total testosterone levels dropped significantly in the cohort treated with metformin (mean difference, 38.3 ng/dl) compared with placebo (mean difference, 0.86 ng/dl) (95% CI, to 0.29 for the difference between group means). In neither group was there a change in the Ferriman and Gallwey scores or acanthosis nigricans (Table 2). Ten of 11 adolescents in the metformin group experienced menses, compared with only 4 of 11 adolescents in the placebo group. The relative risk of menses was statistically significant at 2.50 (95% CI, 1.12 to 5.58). GLUCOSE AND INSULIN PROFILES Fasting blood glucose levels were all normal at baseline and remained this way at the end of the study regardless of the treatment prescribed. None of the study recruits were diabetic based on the American Diabetes Association interpretation of the OGTT, a requirement for participation. The baseline OGTTs revealed that 1 adolescent in the metformin arm had impaired glucose tolerance. At the end of the study, her results demonstrated persistent impaired glucose tolerance. None of the girls in the placebo arm had any glucose intolerance at baseline, but 1 developed this at the end of the 12-week interval. The areas under the insulin response curve were elevated and were not significantly different in the 2 arms at baseline. With metformin, there was a suggestive trend toward lower insulin AUC with treatment, but this was not statistically significant (with metformin, insulin AUC declined by 3662 µu/ml minute, compared with 2093 µu/ml minute for placebo; 95% CI, to 6024 for the mean difference between groups). Homeostasis model assessment calculations also showed a trend toward improved insulin sensitivity with metformin treatment, but again these were not statistically significant (HOMA: metformin therapy [mean difference, 1.06] compared with placebo [mean difference, 0.86] [95% CI, 9.26 to 5.42 for the difference between group means]; QUICKI: metformin therapy [mean difference, 0.00] compared with placebo [mean difference, 0.01] [95% CI, 0.03 to 0.05 for the difference between group means]). LIPID PROFILE On average, the baseline levels of total, low-density lipoprotein and HDL cholesterol were normal for all participants (Table 1). At the end of the trial, metformin had significantly improved HDL cholesterol concentrations compared with placebo. The increase with treatment was 6.98 mg/dl vs 2.33 mg/dl for placebo (95% CI, 0.78 to for the mean difference between groups). Other cholesterol parameters were not significantly different (Table 2). Triglycerides were elevated in both baseline cohorts and fell with metformin treatment, but this did not reach statistical significance. No decline was noted with placebo. All young women were in the follicular phase of their menstrual cycle at baseline; 1 participant 243

4 Table 2. Change From Baseline in Clinical Characteristics and Blood Tests After Administration of Metformin or Placebo for 12 Weeks Mean Difference Characteristic Metformin Group Placebo Group 95% Confidence Interval for the Mean Difference Between Groups Body mass index* to 0.32 Ferriman and Gallwey score 0 0 NA Total testosterone, ng/dl to 0.29 Insulin AUC, µu/ml» min to 6024 Fasting glucose, mg/dl to 5.22 HOMA to 5.42 QUICKI to 0.05 Total cholesterol, mg/dl to LDL cholesterol, mg/dl to HDL cholesterol, mg/dl to Triglycerides, mg/dl to Girls with restored menses, No. 10/11 4/ to 5.58 (relative risk, 2.50) Abbreviations: AUC, area under the curve; HDL, high-density lipoprotein; HOMA, homeostasis model assessment; LDL, low-density lipoprotein; NA, not applicable; QUICKI, quantitative insulin sensitivity check index. *Body mass index is calculated as weight in kilograms divided by the square of height in meters. in the placebo group was excluded from the comparisons because she was in the luteal phase at completion of the study. COMPLIANCE Pill counts were performed at the end of the trial. Two adolescents in the placebo arm admitted to not taking all the required tablets. One of them stated that they caused gastrointestinal adverse effects and discontinued the pills after taking about 45% of them. The second participant did not return for the second OGTT and stated that she only took about 50% of the pills. In the metformin treatment arm, 5 of 11 admitted to poor compliance (defined as 70% of pills taken), citing gastrointestinal disturbances. Based on pill counts, 8% to 60% of the metformin tablets were not consumed. Of note, in the metformin group, all of the noncompliant subjects and 6 of 7 compliant subjects had least 1 menses during the study period; therefore, metformin treatment was associated with restoration of menses in 10 of 11 treated subjects. COMMENT To our knowledge, this is the first study to evaluate the effect of metformin and lifestyle counseling on insulin levels, testosterone levels, and menstrual patterns in adolescent girls with hyperinsulinemia and PCOS in a randomized, double-blind, placebo-controlled study. We found that metformin resulted in a clinically significant impact on menses, significantly reduced serum testosterone levels, and significantly increased HDL cholesterol levels. All of our study subjects were overweight or obese at baseline and did not achieve a significant reduction in BMI with lifestyle intervention and metformin or placebo. In the 2 published pediatric, unrandomized, and uncontrolled studies using metformin for PCOS, a moderate reduction in weight was noted. Both studies by Glueck et al 25 and Arslanian et al 26 were small in sample size, and in the latter, the BMI was significantly lowered because of a significant increase in height. Two small, randomized, double-blind, placebo-controlled trials by Kay et al 27 and Freemark and Bursey 28 evaluated metformin in obese adolescents (not with PCOS) and showed that those treated with metformin had a significantly greater weight loss compared with the placebo groups, although the differences were modest. Of note, the subjects in the Kay et al 27 and Freemark and Bursey 28 studies were also prescribed weight-reduction diets. The adult literature is equally inconclusive with regard to weight reduction, which suggests that metformin may not contribute to weight loss directly. Metformin did, however, result in a significant reduction in total testosterone levels compared with placebo. Our study design could not determine whether reduced androgen levels were a consequence of the downward trend in insulin levels or some other biologic effects of metformin. Given the duration of the study, it comes as little surprise that this did not necessarily translate into a change in degree of hirsutism. Two earlier studies in overweight adolescents with PCOS demonstrated conflicting results; Arslanian et al 26 described significant reductions in both free and total testosterone levels with metformin, and Glueck et al 25 noted a nonsignificant downward trend in testosterone levels. In both studies, moderate weight loss was noted and only in the former study were insulin levels measured, which were decreased with metformin. The small (n=10) study by Ibanez et al 29 in nonobese adolescent girls with PCOS observed a decrease in both androgen levels and hirsutism with metformin, associated with a concomitant decrease in insulin concentrations and without any weight loss. We found a significant rise in HDL cholesterol levels in the metformin group compared with the placebo group by the end of the study period. We did not find significant changes in other lipid parameters. The mean triglyceride concentrations were elevated in both study groups at the start of the study, with a nonsignificant re- 244

5 duction in triglyceride levels observed in the metformintreated group alone. Again, the impact of metformin in adolescents has been inconsistent. Glueck et al 25 observed a significant reduction in total cholesterol levels in a nonrandomized study while Arslanian et al 26 saw no significant change in cholesterol or triglyceride levels. In the randomized double-blind placebo-controlled trial by Kay et al 27 of obese adolescents (not with PCOS), a significant improvement in the lipid profile was seen with metformin treatment (decreased total cholesterol and triglyceride levels). In all 3 of these studies, metformin treatment was associated with weight loss. In 2 of these studies, insulin levels were assessed and found to be reduced with metformin. Most importantly, menses was 2.5 times more likely to occur in the metformin group compared with the placebo group. Metformin has been deemed to be beneficial in the restoration of menses in other studies, from 40% to 100% (40%, Glueck et al 25 ; 91%, Arslanian et al 26 ; and 100%, Ibanez et al 29 ). Indeed, Ibanez et al confirmed that menses returned, and the majority of these cycles were ovulatory. 30,31 We noted an improvement in measures of insulin sensitivity, including the insulin AUC and HOMA, in the adolescents treated with metformin, but not in the control group. However, this trend failed to achieve statistical significance, possibly reflecting sample size and inherent variability of the measure. Because some clinical benefits were noted, we postulate that this reduction may have had biologic effects. Earlier studies by Arslanian et al 26 and Ibanez et al 30,31 in adolescents with PCOS have demonstrated a significant decline in insulin levels assessed by both fasting insulin-glucose ratios and formal pharmacokinetic AUC. However, most studies in female and male adolescents have also demonstrated a decrease in BMI or body weight with metformin treatment, which could conceivably alter insulin sensitivity Poor compliance in nearly half (5/11) of the adolescents randomized to metformin and/or our small sample size may have limited the ability to detect statistically significant differences in insulin concentrations despite the clinical impact on menses and testosterone levels. Comparing our results with the other trials carried out in adolescents suggests that important biologic effects can occur without weight loss and without statistically significant improvements in measures of insulin sensitivity. This raises the question as to whether metformin has additional biologic actions besides that of an insulin sensitizer or whether the impact of metformin is noted with very small decreases in insulin concentrations. The use of insulin-sensitizing agents, especially metformin, has generally been accepted in adult women with PCOS although not all studies have demonstrated consistent benefits. 13 In adolescents with PCOS, there have been fewer studies of metformin, and controversy remains with regards to its benefits and mechanisms of action. It is difficult to directly compare our study with those previously published because of the inherent differences in study design, although most appear to demonstrate that metformin treatment leads to clinical improvement. The goal of this therapy in the adolescent is more likely to focus on the reduction of hirsutism and acne with return of menses as a less important outcome. Fertility and long-term cardiovascular health appear more remote concerns for these young adults. Our randomized placebo-controlled study, albeit small in sample size, demonstrated a significant decrease in testosterone levels and an increase in the frequency of menses in adolescents with oligomenorrhea or amenorrhea. Measures of insulin sensitivity improved, although not significantly. Poor compliance with metformin and/or sample size were major limitations as was the short duration of the study. Despite other adolescent preoccupations, the increased frequency of menses observed with a combination of metformin and lifestyle therapy may benefit uterine health. In addition, the significant increase in HDL cholesterol levels may benefit overall health in the future, as might the trend toward improvement in insulin AUC, although longer term follow-up studies will be needed to explore these questions. Accepted for Publication: September 13, Corresponding Author: Tracey Bridger, MD, FRCPC, Paediatric Endocrinology, Janeway Child Health and Rehabilitation Centre, 300 Prince Philip Dr, St John s, Newfoundland and Labrador, Canada A1B 3V6 Funding/Support: Montreal Children s Hospital Research Institute, Montreal, Quebec, and Aventis Pharmaceutical, Laval, Quebec, supplied metformin and an identical appearing placebo. REFERENCES 1. Pfeifer SM, Dayal M. Treatment of the adolescent patient with polycystic ovary syndrome. Obstet Gynecol Clin North Am. 2003;30: Zawadzki JK, Dunaif A. Diagnostic criteria for polycystic ovary syndrome: towards a rational approach. In: Dunaif A, Givens JR, Haseltine FP, Merriam GR, eds. Current Issues in Endocrinology and Metabolism: Polycystic Ovary Syndrome. Boston, Mass: Blackwell Scientific; 1992: Knochenhauer ES, Key TJ, Kahsar-Miller M, Waggoner W, Boots LR, Azziz R. 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6 tential by lifestyle modification in obese polycystic ovary syndrome: role of insulin sensitivity and luteinizing hormone. J Clin Endocrinol Metab. 1999;84: Taylor AE. Insulin lowering medications in polycystic ovary syndrome. Obstet Gynecol Clin North Am. 2000;27: Kent SC, Legro RS. Polycystic ovary syndrome in adolescents. Adolesc Med. 2002; 13: Apter D. Endocrine and metabolic abnormalities in adolescents with a PCOSlike condition: consequences for adult reproduction. Trends Endocrinol Metab. 1998;9: Lewy VD, Danadian K, Witchel SF, Arslanian S. Early metabolic abnormalities in adolescent girls with polycystic ovarian syndrome. J Pediatr. 2001;138: Silfen ME. Early endocrine, metabolic, and sonographic characteristics of polycystic ovary syndrome (PCOS): comparison between nonobese and obese adolescents. J Clin Endocrinol Metab. 2003;88: Dean H. Metformin use in adolescent with PCOS: an invited editorial from the pediatric endocrinology perspective. J Pediatr Adolesc Gynecol. 2003;16: Expert Committee on the Diagnosis and Classification of Diabetes Mellitus. Report of the Expert Committee on the Diagnosis and Classification of Diabetes Mellitus. Diabetes Care. 2000;23:S5-S Ferriman D, Gallwey JD. Clinical assessment of body hair growth in women. J Clin Endocrinol Metab. 1961;21: Ciampelli M. Assessment of insulin sensitivity from measurements in the fasting state and during an oral glucose tolerance test in polycystic ovary syndrome and menopausal patients. J Clin Endocrinol Metab. 2005;90: Skrha J, Haas T, Sindelka G, et al. Comparison of the insulin action parameters from hyperinsulinemic clamps with homeostasis model assessment and QUICKI indexes in subjects with different endocrine disorders. J Clin Endocrinol Metab. 2004;89: Katz A, Nambi SS, Mather K, et al. Quantitative insulin sensitivity check index: a simple, accurate method for assessing insulin sensitivity in humans. J Clin Endocrinol Metab. 2000;85: Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia. 1985; 28: Glueck CJ, Wang P, Fontaine R, Tracy T, Sieve-Smith L. Metformin to restore normal menses in oligo-amenorrheic teenage girls with polycystic ovary syndrome (PCOS). J Adolesc Health. 2001;29: Arslanian SA, Lewy V, Danadian K, Saad R. Metformin therapy in obese adolescents with polycystic ovary syndrome and impaired glucose intolerance: amelioration of exaggerated adrenal response to adrenocorticotropin with reduction of insulinemia/insulin resistance. J Clin Endocrinol Metab. 2002;87: Kay JP, Alemzadeh R, Langley G, D Angelo L, Smith P, Holshouser S. Beneficial effects of metformin in normoglycemic morbidly obese adolescents. Metabolism. 2001;50: Freemark M, Bursey D. The effects of metformin on body mass index and glucose tolerance in obese adolescents with fasting hyperinsulinemia and a family history of type 2 diabetes. Pediatrics. 2001;107:E Ibanez L, Valls C, Potau N, Marcos MV, de Zegher F. Sensitization to insulin in adolescent girls to normalize hirsutism, hyperandrogenism, oligomenorrhea, dyslipidemia, and hyperinsulinism after precocious pubarche. J Clin Endocrinol Metab. 2000;85: Ibanez L, Valls C, Ferrer A, Marcos MV, Rodriguez-Hierro F, de Zegher F. Sensitization to insulin induces ovulation in nonobese adolescents with anovulatory hyperandrogenism. J Clin Endocrinol Metab. 2001;86: Ibanez L, Potau N, Ferrer A, Rodriguez-Hierro F, Marcos MV, de Zegher F. Anovulation in eumenorrheic, nonobese adolescent girls born small for gestational age: insulin sensitization induces ovulation, increases lean body mass, and reduces abdominal fat excess, dyslipidemia, and subclinical hyperandrogenism. J Clin Endocrinol Metab. 2002;87: Announcement Submissions. The Editors welcome contributions to Picture of the Month. Submissions should describe common problems presenting uncommonly, rather than total zebras. Cases should be of interest to practicing pediatricians, highlighting problems that they are likely to at least occasionally encounter in the office or hospital setting. High-quality clinical images (in either 35-mm slide or electronic format) along with parent or patient permission to use these images must accompany the submission. The entire discussion should comprise no more than 750 words. Articles and photographs accepted for publication will bear the contributor s name. There is no charge for reproduction and printing of color illustrations. For details regarding electronic submission, please see: 246