Supplementary Figure 1.
|
|
- Colin Simon
- 5 years ago
- Views:
Transcription
1 Supplementary Figure 1. Increased expression of cell cycle pathway genes in insulin + Glut2 low cells of STZ-induced diabetic islets. A) random blood glucose measuers of STZ and vehicle treated MIP-GFP transgenic mice on day 5 after treatment. B) FPKM values for leading edge genes of cell cycle pathway increased in diabetic insulin + Glut2 low cells.
2 Supplementary Figure 2. Expansion of human diabetic PMP population and their increased proliferation capacity is maintained throughout multiple passaging. Frequencies and diameters of clonal spheres produced from individual healthy (n = 1) and diabetic (n = 3) donors in secondary (2 ) and tertiary (3 ) passages. The primary (1 ) spheres produced after 2 week culture of primary islet cells were handpicked, pooled, dissociated and cultured at clonal density (5000 cells/ml) to form secondary spheres (A). The same procedure was repeated on 2 spheres after two weeks to produce 3 spheres (B). The individual data from diabetic donors were combined and compared with the single non-diabetic control. Two way ANOVA showed a significant interaction of diabetes and sphere diameter in both passages (F 2 (2,231) = 20.35, p<0.0001, and F 3 (2,108) = 28.21, p<0.001) with a main effect of diabetes on the total number of spheres(f 2 (1,231) = 44.63, p<0.0001, and F 3 (1,108) = 22.07, p<0.001). N = observed wells/group and values indicate means +/- SEMs.
3 Supplementary Figure 3. The proportion of hormone + cells produced from human PMP single colonies increases in diabetic condition. Single clonal spheres produced from three type 1 diabetic (D) and two control (C) human pancreatic islets were differentiated in serum containing media for 2 weeks and were stained for insulin (A) or glucagon (B) expression. (A) Clonal spheres from diabetic samples produced greater numbers of insulin + cells (One way ANOVA, F (4,53) = 5.024, p = 0017). Tukey post test revealed a significant increase of insulin + cell production by D2 and D3 spheres (p<0.05). (B) Spherical colonies from D1 and D3 produced significantly higher proportion of glucagon + cells (One way ANOVA, F (4,68) = 9.701, p<0.0001, Tukey post-test, p<0.05). Number of analyzed spherical colonies is per sample in both experiments. Values indicate means+/-sems.
4 Supplementary Figure 4. PMP population expands in aged human islets. Dissociated pancreatic islet cells from 4 different donors (43, 45, 65, and 68 years old) were cultured at clonal density to form clonal spheres. The frequencies and diameters of spherical colonies were assessed for each sample. Values indicate means+/-sems
5 Supplementary Table 1. Log2 FDR values for comparing the KEGG and REACTOME defined biological pathways of insulin + Glut2 low versus insulin + Glut2 high cells from non-diabetic MIP-GFP transgenic islets at p<0.05 Pathway Glut2 low vs Glut2 high (CTRL) REACTOME Neuronal System KEGG Oxidative Phosphorylation REACTOME Respiratory Electron Transport ATP Synthesis by Chemiosmotic Coupling and Heat Production by Uncoupling Proteins REACTOME Metabolism Of Proteins REACTOME Transmission Across Chemical Synapses KEGG Parkinsons Disease REACTOME Respiratory Electron Transport REACTOME Regulation Of Gene Expression In Beta Cells KEGG Maturity Onset Diabetes Of The Young KEGG Huntingtons Disease KEGG Protein Export REACTOME Regulation Of Insulin Secretion REACTOME Tca Cycle and Respiratory Electron Transport REACTOME Synthesis Secretion and Inactivation Of GIP REACTOME Diabetes Pathways REACTOME Asparagine N Linked Glycosylation REACTOME Regulation Of Beta Cell Development REACTOME Integration Of Energy Metabolism KEGG_Alzheimers Disease REACTOME Unfolded Protein Response REACTOME Neurotransmitter Release Cycle REACTOME Glucose Metabolism REACTOME Gaba Synthesis Release Reuptake and Degradation REACTOME N Glycan Trimming In The ER and Calnexin Calreticulin Cycle
6 REACTOME Incretin Synthesis Secretion and Inactivation REACTOME Post Translational Protein Modification REACTOME Activation Of Chaperone Genes by Xbp1s REACTOME Calnexin Calreticulin Cycle REACTOME Membrane Trafficking REACTOME Synthesis Secretion and Inactivation Of GLP REACTOME SRP Dependent Co-translational Protein Targeting To Membrane KEGG Cardiac Muscle Contraction KEGG Valine Leucine and Isoleucine Degradation REACTOME Neurotransmitter Receptor Binding and Downstream Transmission In The Postsynaptic Cell REACTOME Cytosolic Sulfonation Of Small Molecules REACTOME Translation REACTOME Glutamate Neurotransmitter Release Cycle REACTOME Il-35 and GM-CSF Signaling KEGG Axon Guidance REACTOME Yap1 and Wwtr1 Taz Stimulated Gene Expression REACTOME PPARA Activates Gene Expression REACTOME Hyaluronan Metabolism REACTOME Cell Extracellular Matrix Interactions REACTOME Class A Rhodopsin Like Receptors REACTOME Peptide Ligand Binding Receptors REACTOME Antigen Activates B-Cell Receptor Leading To Generation Of Second Messengers REACTOME Initial Triggering Of Complement REACTOME Immunoregulatory Interactions Between A Lymphoid and A Non Lymphoid Cell KEGG Nod Like Receptor Signaling Pathway
7 REACTOME GPVI Mediated Activation Cascade REACTOME Toll Receptor Cascades REACTOME Transcriptional Regulation Of White Adipocyte Differentiation REACTOME Rig I Mda5 Mediated Induction Of IFN- Alpha Beta Pathways REACTOME Glycosaminoglycan Metabolism REACTOME TRAF6 Mediated IRF7 Activation KEGG T Cell Receptor Signaling Pathway KEGG Natural Killer Cell Mediated Cytotoxicity REACTOME Signaling by Rho GTPases KEGG Rig I Like Receptor Signaling Pathway KEGG Leukocyte Transendothelial Migration REACTOME Chemokine Receptors Bind Chemokines REACTOME Platelet Activation Signaling and Aggregation KEGG TGF Beta Signaling Pathway KEGG Cytosolic DNA Sensing Pathway REACTOME Semaphorin Interactions KEGG Autoimmune Thyroid Disease KEGG Primary Immunodeficiency KEGG JAK-STAT Signaling Pathway KEGG Fc Epsilon RI Signaling Pathway KEGG Chemokine Signaling Pathway REACTOME Developmental Biology REACTOME Axon Guidance REACTOME Cell Surface Interactions At The Vascular Wall KEGG Asthma REACTOME Integrin Cell Surface Interactions REACTOME Signaling by ILS KEGG Fc Gamma R Mediated Phagocytosis
8 KEGG Allograft Rejection REACTOME Interferon Alpha Beta Signaling KEGG Regulation Of Actin Cytoskeleton REACTOME Collagen Formation KEGG Graft Versus Host Disease REACTOME Interferon Signaling KEGG B-Cell Receptor Signaling Pathway REACTOME Interferon Gamma Signaling KEGG Pathways In Cancer KEGG ECM Receptor Interaction KEGG Complement and Coagulation Cascades REACTOME Immune System REACTOME Extracellular Matrix Organization KEGG Focal Adhesion KEGG Toll Like Receptor Signaling Pathway REACTOME Innate Immune System REACTOME Hemostasis KEGG Intestinal Immune Network For IgA Production KEGG Leishmania Infection REACTOME Cytokine Signaling In Immune System KEGG Hematopoietic Cell Lineage KEGG Cytokine Cytokine Receptor Interaction
9 Supplementary Table 2. KEGG and REACTOME defined pathways with significant alteration in insulin + glut2 low cells of STZ-induced diabetic islets. Pathway Glut2 low vs. Glut2 high Glut2 low vs. Glut2 high (Ctrl) (STZ) REACTOME Potassium Channels NA REACTOME Interaction Between L1 and Ankyrins NA REACTOME Amine Derived Hormones NA REACTOME Amine Ligand Binding Receptors NA REACTOME Cell Cycle NA KEGG P53 Signaling Pathway NA REACTOME Inflammasomes NA REACTOME NCAM1 Interactions NA Values are log2 FDR at p<0.05
REACTOME: Nonsense Mediated Decay (NMD) REACTOME:72764 Eukaryotic Translation Termination. REACTOME:72737 Cap dependent Translation Initiation
A REACTOME:975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) REACTOME:975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) REACTOME:927802
More informationSupplementary data Table S3. GO terms, pathways and networks enriched among the significantly correlating genes using Tox-Profiler
Supplementary data Table S3. GO terms, pathways and networks enriched among the significantly correlating genes using Tox-Profiler DR CALUX Boys Girls Database Systemic lupus erythematosus 4.4 0.0021 6.7
More informationPathways of changes in hypermethylation in F1 offspring exposed to intrauterine hyperglycemia
Supplementary Table 1. Pathways of changes in hypermethylation in F1 offspring exposed to intrauterine hyperglycemia Pathway Enrichment Score (-log10[p value]) p-value genes Glycosaminoglycan degradation
More informationPrinciples of Genetics and Molecular Biology
Cell signaling Dr. Diala Abu-Hassan, DDS, PhD School of Medicine Dr.abuhassand@gmail.com Principles of Genetics and Molecular Biology www.cs.montana.edu Modes of cell signaling Direct interaction of a
More informationSupplementary Figure 1:
Supplementary Figure 1: (A) Whole aortic cross-sections stained with Hematoxylin and Eosin (H&E), 7 days after porcine-pancreatic-elastase (PPE)-induced AAA compared to untreated, healthy control aortas
More informationLate regulation of immune genes and micrornas in circulating leukocytes in a pig model of
1 Supplementary material for: 2 3 4 5 6 Late regulation of immune genes and micrornas in circulating leukocytes in a pig model of influenza A (H1N2) infection Louise Brogaard, Peter M. H. Heegaard, Lars
More informationIdentification of Tissue-Specific Protein-Coding and Noncoding. Transcripts across 14 Human Tissues Using RNA-seq
Identification of Tissue-Specific Protein-Coding and Noncoding Transcripts across 14 Human Tissues Using RNA-seq Jinhang Zhu 1, Geng Chen 1, Sibo Zhu 1,2, Suqing Li 3, Zhuo Wen 3, Bin Li 1, Yuanting Zheng
More informationSubject Index. Bcl-2, apoptosis regulation Bone marrow, polymorphonuclear neutrophil release 24, 26
Subject Index A1, apoptosis regulation 217, 218 Adaptive immunity, polymorphonuclear neutrophil role 31 33 Angiogenesis cancer 178 endometrium remodeling 172 HIV Tat induction mechanism 176 inflammatory
More informationCELL BIOLOGY - CLUTCH CH THE IMMUNE SYSTEM.
!! www.clutchprep.com CONCEPT: OVERVIEW OF HOST DEFENSES The human body contains three lines of against infectious agents (pathogens) 1. Mechanical and chemical boundaries (part of the innate immune system)
More informationChapter 11 CYTOKINES
Chapter 11 CYTOKINES group of low molecular weight regulatory proteins secreted by leukocytes as well as a variety of other cells in the body (8~30kD) regulate the intensity and duration of the immune
More information3.D- Cell Communication
3.D- Cell Communication Big Idea 3: Living systems store, retrieve, transmit and respond to information essential to life processes. EU 3.A: Heritable information provides for continuity of life. EU 3.B:
More informationChapter 13: Cytokines
Chapter 13: Cytokines Definition: secreted, low-molecular-weight proteins that regulate the nature, intensity and duration of the immune response by exerting a variety of effects on lymphocytes and/or
More informationSupplementary information for: Community detection for networks with. unipartite and bipartite structure. Chang Chang 1, 2, Chao Tang 2
Supplementary information for: Community detection for networks with unipartite and bipartite structure Chang Chang 1, 2, Chao Tang 2 1 School of Life Sciences, Peking University, Beiing 100871, China
More informationThe Immune System. A macrophage. ! Functions of the Immune System. ! Types of Immune Responses. ! Organization of the Immune System
The Immune System! Functions of the Immune System! Types of Immune Responses! Organization of the Immune System! Innate Defense Mechanisms! Acquired Defense Mechanisms! Applied Immunology A macrophage
More informationMedical Virology Immunology. Dr. Sameer Naji, MB, BCh, PhD (UK) Head of Basic Medical Sciences Dept. Faculty of Medicine The Hashemite University
Medical Virology Immunology Dr. Sameer Naji, MB, BCh, PhD (UK) Head of Basic Medical Sciences Dept. Faculty of Medicine The Hashemite University Human blood cells Phases of immune responses Microbe Naïve
More informationCells communicate with each other via signaling ligands which interact with receptors located on the surface or inside the target cell.
BENG 100 Frontiers of Biomedical Engineering Professor Mark Saltzman Chapter 6 SUMMARY In this chapter, cell signaling was presented within the context of three physiological systems that utilize communication
More information2013 W. H. Freeman and Company. 12 Signal Transduction
2013 W. H. Freeman and Company 12 Signal Transduction CHAPTER 12 Signal Transduction Key topics: General features of signal transduction Structure and function of G protein coupled receptors Structure
More informationfasting blood glucose [mg/dl]
SUPPLEMENTL MTERIL Supplemental Figure I body weight [g] 5 5 5 fasting blood glucose [mg/dl] 5 5 5 C total cholesterol [mg/dl] 8 6 4 WT Has -/- Supplemental Figure I: Has-deficient mice exhibited no apparent
More informationAntigen Presentation and T Lymphocyte Activation. Abul K. Abbas UCSF. FOCiS
1 Antigen Presentation and T Lymphocyte Activation Abul K. Abbas UCSF FOCiS 2 Lecture outline Dendritic cells and antigen presentation The role of the MHC T cell activation Costimulation, the B7:CD28 family
More informationIntrinsic cellular defenses against virus infection
Intrinsic cellular defenses against virus infection Detection of virus infection Host cell response to virus infection Interferons: structure and synthesis Induction of antiviral activity Viral defenses
More informationPCxN: The pathway co-activity map: a resource for the unification of functional biology
PCxN: The pathway co-activity map: a resource for the unification of functional biology Sheffield Institute for Translational Neurosciences Center for Integrative Genome Translation GENOME INFORMATICS
More informationHow Will New Therapies Affect HCC Development?
How Will New Therapies Affect HCC Development? July 6, 2018 Scott Friedman, M.D. Fishberg Professor of Medicine Dean for Therapeutic Discovery Chief, Division of Liver Diseases Icahn School of Medicine
More informationNewly Recognized Components of the Innate Immune System
Newly Recognized Components of the Innate Immune System NOD Proteins: Intracellular Peptidoglycan Sensors NOD-1 NOD-2 Nod Protein LRR; Ligand Recognition CARD RICK I-κB p50 p65 NF-κB Polymorphisms in Nod-2
More information# of transcr ipts FDR q- value transcripts PGF,CTNNB1,AKT2,FARP2,VEGFB,PDGFRA,DRD 2,CSF1,CDH1,ITGB1,SRC,KRAS,RASGRP3,MAP2. Pathway
Supplementary Table 9. Pathways and biological processes associated with transcripts specific to SEB in both the pre- and post- RASP leukocytes (transcripts included in the Reactome Function Interaction
More informationsilent epidemic,. (WHO),
Tel: 02-740-8686; E-mail: hhbkim@snu.ac.kr silent epidemic,. (WHO),. 5 3, 1. 50 70. 50%, 25%, 20% (12~35%). 2.8% 0.7% 4. ( ). bone remodeling (osteoblast), (osteoclast),.. 3~4.. 70% (osteocyte) (bone lining
More informationCYTOKINE RECEPTORS AND SIGNAL TRANSDUCTION
CYTOKINE RECEPTORS AND SIGNAL TRANSDUCTION What is Cytokine? Secreted popypeptide (protein) involved in cell-to-cell signaling. Acts in paracrine or autocrine fashion through specific cellular receptors.
More informationEffector T Cells and
1 Effector T Cells and Cytokines Andrew Lichtman, MD PhD Brigham and Women's Hospital Harvard Medical School 2 Lecture outline Cytokines Subsets of CD4+ T cells: definitions, functions, development New
More informationPrinciples of Adaptive Immunity
Principles of Adaptive Immunity Chapter 3 Parham Hans de Haard 17 th of May 2010 Agenda Recognition molecules of adaptive immune system Features adaptive immune system Immunoglobulins and T-cell receptors
More informationAnimal Models to Understand Immunity
Animal Models to Understand Immunity Hussein El Saghire hesaghir@sckcen.be Innate Adaptive immunity Immunity MAPK and NF-kB TLR pathways receptors Fast Slow Non-specific Specific NOD-like receptors T-cell
More informationHormones. Prof. Dr. Volker Haucke Institut für Chemie-Biochemie Takustrasse 6
Hormones Prof. Dr. Volker Haucke Institut für Chemie-Biochemie Takustrasse 6 Tel. 030-8385-6920 (Sekret.) 030-8385-6922 (direkt) e-mail: vhaucke@chemie.fu-berlin.de http://userpage.chemie.fu-berlin.de/biochemie/aghaucke/teaching.html
More informationT Cell Activation, Costimulation and Regulation
1 T Cell Activation, Costimulation and Regulation Abul K. Abbas, MD University of California San Francisco 2 Lecture outline T cell antigen recognition and activation Costimulation, the B7:CD28 family
More informationSystems biology approaches and pathway tools for investigating cardiovascular disease
Systems biology approaches and pathway tools for investigating cardiovascular disease Craig E. Wheelock 1,2*, Åsa M. Wheelock 2,3,4, Shuichi Kawashima 5, Diego Diez 2, Minoru Kanehisa 2,5, Marjan van Erk
More informationHORMONES (Biomedical Importance)
hormones HORMONES (Biomedical Importance) Hormones are the chemical messengers of the body. They are defined as organic substances secreted into blood stream to control the metabolic and biological activities.
More informationTCR, MHC and coreceptors
Cooperation In Immune Responses Antigen processing how peptides get into MHC Antigen processing involves the intracellular proteolytic generation of MHC binding proteins Protein antigens may be processed
More informationReceptors Functions and Signal Transduction- L4- L5
Receptors Functions and Signal Transduction- L4- L5 Faisal I. Mohammed, MD, PhD University of Jordan 1 PKC Phosphorylates many substrates, can activate kinase pathway, gene regulation PLC- signaling pathway
More informationT Cell Activation. Patricia Fitzgerald-Bocarsly March 18, 2009
T Cell Activation Patricia Fitzgerald-Bocarsly March 18, 2009 Phases of Adaptive Immune Responses Phases of T cell responses IL-2 acts as an autocrine growth factor Fig. 11-11 Clonal Expansion of T cells
More informationCell Quality Control. Peter Takizawa Department of Cell Biology
Cell Quality Control Peter Takizawa Department of Cell Biology Cellular quality control reduces production of defective proteins. Cells have many quality control systems to ensure that cell does not build
More informationT-cell activation T cells migrate to secondary lymphoid tissues where they interact with antigen, antigen-presenting cells, and other lymphocytes:
Interactions between innate immunity & adaptive immunity What happens to T cells after they leave the thymus? Naïve T cells exit the thymus and enter the bloodstream. If they remain in the bloodstream,
More informationT-cell activation T cells migrate to secondary lymphoid tissues where they interact with antigen, antigen-presenting cells, and other lymphocytes:
Interactions between innate immunity & adaptive immunity What happens to T cells after they leave the thymus? Naïve T cells exit the thymus and enter the bloodstream. If they remain in the bloodstream,
More informationChapter 3 The Induced Responses of Innate Immunity
Chapter 3 The Induced Responses of Innate Immunity Pattern recognition by cells of the innate immune system Pattern recognition by cells of the innate immune system 4 main pattern recognition receptors
More informationThe Adaptive Immune Responses
The Adaptive Immune Responses The two arms of the immune responses are; 1) the cell mediated, and 2) the humoral responses. In this chapter we will discuss the two responses in detail and we will start
More informationAdaptive immune responses: T cell-mediated immunity
MICR2209 Adaptive immune responses: T cell-mediated immunity Dr Allison Imrie allison.imrie@uwa.edu.au 1 Synopsis: In this lecture we will discuss the T-cell mediated immune response, how it is activated,
More informationCell-Derived Inflammatory Mediators
Cell-Derived Inflammatory Mediators Introduction about chemical mediators in inflammation Mediators may be Cellular mediators cell-produced or cell-secreted derived from circulating inactive precursors,
More informationT Lymphocyte Activation and Costimulation. FOCiS. Lecture outline
1 T Lymphocyte Activation and Costimulation Abul K. Abbas, MD UCSF FOCiS 2 Lecture outline T cell activation Costimulation, the B7:CD28 family Inhibitory receptors of T cells Targeting costimulators for
More informationMechanisms of Cell Injury: Loss of Calcium Homeostasis
Mechanisms of Cell Injury: Loss of Calcium Homeostasis SCPA610: Cellular Pathology Amornrat N. Jensen, Ph.D. amornrat.nar@mahidol.ac.th Leading questions Why is intracellular calcium important for the
More informationCell Biology (BIOL 4374 and BCHS 4313) Third Exam 4/24/01
Cell Biology (BIOL 4374 and BCHS 4313) Third Exam 4/24/01 Name SS# This exam is worth a total of 100 points. The number of points each question is worth is shown in parentheses. For multiple choice questions,
More informationT cell-mediated immunity
T cell-mediated immunity Overview For microbes within phagosomes in phagocytes.cd4+ T lymphocytes (TH1) Activate phagocyte by cytokines studies on Listeria monocytogenes For microbes infecting and replicating
More informationCell Signaling (part 1)
15 Cell Signaling (part 1) Introduction Bacteria and unicellular eukaryotes respond to environmental signals and to signaling molecules secreted by other cells for mating and other communication. In multicellular
More informationACTIVATION OF T LYMPHOCYTES AND CELL MEDIATED IMMUNITY
ACTIVATION OF T LYMPHOCYTES AND CELL MEDIATED IMMUNITY The recognition of specific antigen by naïve T cell induces its own activation and effector phases. T helper cells recognize peptide antigens through
More informationImmunology Basics Relevant to Cancer Immunotherapy: T Cell Activation, Costimulation, and Effector T Cells
Immunology Basics Relevant to Cancer Immunotherapy: T Cell Activation, Costimulation, and Effector T Cells Andrew H. Lichtman, M.D. Ph.D. Department of Pathology Brigham and Women s Hospital and Harvard
More informationProtein Trafficking in the Secretory and Endocytic Pathways
Protein Trafficking in the Secretory and Endocytic Pathways The compartmentalization of eukaryotic cells has considerable functional advantages for the cell, but requires elaborate mechanisms to ensure
More informationBasis of Immunology and
Basis of Immunology and Immunophysiopathology of Infectious Diseases Jointly organized by Institut Pasteur in Ho Chi Minh City and Institut Pasteur with kind support from ANRS & Université Pierre et Marie
More informationThanks to: Signal Transduction. BCB 570 "Signal Transduction" 4/8/08. Drena Dobbs, ISU 1. An Aging Biologist s. One Biologist s Perspective
BCB 570 "" Thanks to: One Biologist s Perspective Drena Dobbs BCB & GDCB Iowa State University Howard Booth Biology Eastern Michigan University for Slides modified from his lecture Cell-Cell Communication
More informationBasics of Pharmacology
Basics of Pharmacology Pekka Rauhala Transmed 2013 What is pharmacology? Pharmacology may be defined as the study of the effects of drugs on the function of living systems Pharmacodynamics The mechanism(s)
More informationCellular Physiology (PHSI3009) Contents:
Cellular Physiology (PHSI3009) Contents: Cell membranes and communication 2 nd messenger systems G-coupled protein signalling Calcium signalling Small G-protein signalling o RAS o MAPK o PI3K RHO GTPases
More informationInnate Immunity. Chapter 3. Connection Between Innate and Adaptive Immunity. Know Differences and Provide Examples. Antimicrobial peptide psoriasin
Chapter Know Differences and Provide Examples Innate Immunity kin and Epithelial Barriers Antimicrobial peptide psoriasin -Activity against Gram (-) E. coli Connection Between Innate and Adaptive Immunity
More informationImmunological Aspects of Parasitic Diseases in Immunocompromised Individuals. Taniawati Supali. Department of Parasitology
Immunological Aspects of Parasitic Diseases in Immunocompromised Individuals Taniawati Supali Department of Parasitology 1 Defense mechanism in human Th17 (? ) Acute Chronic Th1 Th 2 Intracellular Treg
More informationLecture Outline. Hormones & Chemical Signaling. Communication Basics: Overview. Communication Basics: Methods. Four methods of cell communication
Lecture Outline Hormones & Chemical Signaling Communication Basics Communication Overview Communication Methods Signal pathways Regulation (modulation) of signal pathways Homeostasis... again Endocrine
More informationAnimal Physiology Study Guide
Animal Physiology Study Guide 1. Which of the following are an example of passive transport? 2. Which active transport? 3. How can you tell? 1. Which of the following are an example of passive transport?
More informationEffector mechanisms of cell-mediated immunity: Properties of effector, memory and regulatory T cells
ICI Basic Immunology course Effector mechanisms of cell-mediated immunity: Properties of effector, memory and regulatory T cells Abul K. Abbas, MD UCSF Stages in the development of T cell responses: induction
More informationACTIVATION AND EFFECTOR FUNCTIONS OF CELL-MEDIATED IMMUNITY AND NK CELLS. Choompone Sakonwasun, MD (Hons), FRCPT
ACTIVATION AND EFFECTOR FUNCTIONS OF CELL-MEDIATED IMMUNITY AND NK CELLS Choompone Sakonwasun, MD (Hons), FRCPT Types of Adaptive Immunity Types of T Cell-mediated Immune Reactions CTLs = cytotoxic T lymphocytes
More informationCytokines modulate the functional activities of individual cells and tissues both under normal and pathologic conditions Interleukins,
Cytokines http://highered.mcgraw-hill.com/sites/0072507470/student_view0/chapter22/animation the_immune_response.html Cytokines modulate the functional activities of individual cells and tissues both under
More informationThird line of Defense
Chapter 15 Specific Immunity and Immunization Topics -3 rd of Defense - B cells - T cells - Specific Immunities Third line of Defense Specific immunity is a complex interaction of immune cells (leukocytes)
More informationFc receptors, phagocytosis role 128
Subject Index Adaptive immunity dependence on innate immunity 9, 10 evolution 10 Aging anti-inflammatory agents in counteraction 202 beneficial polymorphisms 199 201 definition 18, 189 innate immunity
More informationGoals and Challenges of Communication. Communication and Signal Transduction. How Do Cells Communicate?
Goals and Challenges of Communication Reaching (only) the correct recipient(s) Imparting correct information Timeliness Causing the desired effect Effective termination Communication and Signal Transduction
More informationG-Protein Signaling. Introduction to intracellular signaling. Dr. SARRAY Sameh, Ph.D
G-Protein Signaling Introduction to intracellular signaling Dr. SARRAY Sameh, Ph.D Cell signaling Cells communicate via extracellular signaling molecules (Hormones, growth factors and neurotransmitters
More informationAyman Mesleh & Leen Alnemrawi. Bayan Abusheikha. Faisal
24 Ayman Mesleh & Leen Alnemrawi Bayan Abusheikha Faisal We were talking last time about receptors for lipid soluble hormones.the general mechanism of receptors for lipid soluble hormones: 1. Receptors
More informationCytokines, adhesion molecules and apoptosis markers. A comprehensive product line for human and veterinary ELISAs
Cytokines, adhesion molecules and apoptosis markers A comprehensive product line for human and veterinary ELISAs IBL International s cytokine product line... is extremely comprehensive. The assays are
More informationThe Lymphatic System and Body Defenses
PowerPoint Lecture Slide Presentation by Patty Bostwick-Taylor, Florence-Darlington Technical College The Lymphatic System and Body Defenses 12PART B Adaptive Defense System: Third Line of Defense Immune
More informationA. Incorrect! It s not correct. Synergism of cytokines refers to two or more cytokines acting together.
Immunology - Problem Drill 11: Cytokine and Cytokine Receptors Question No. 1 of 10 1. A single cytokine can act on several different cell types, which is known as. Question #1 (A) Synergism (B) Pleiotropism
More informationIntercellular indirect communication
Intercellular indirect communication transmission of chemical signals: sending cell signal transmitting tissue hormone medium receiving cell hormone intercellular fluid blood neurocrine neurotransmitter
More informationSupplementary Figure 1: Func8onal Network Analysis of Kinases Significantly Modulated by MERS CoV Infec8on and Conserved Across All Time Points
A. B. 8 4 Supplementary Figure : Func8onal Network Analysis of Kinases Significantly Modulated by MERS CoV Infec8on and Conserved Across All Time Points Examined. A) Venn diagram analysis of kinases significantly
More informationSUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION Advances in pancreatic islet monolayer culture on glass surfaces enable superresolution microscopy and insights into beta cell ciliogenesis and proliferation Edward A. Phelps,
More informationInnate immunity. Abul K. Abbas University of California San Francisco. FOCiS
1 Innate immunity Abul K. Abbas University of California San Francisco FOCiS 2 Lecture outline Components of innate immunity Recognition of microbes and dead cells Toll Like Receptors NOD Like Receptors/Inflammasome
More informationDiscussion & Conclusion
Discussion & Conclusion 7. Discussion DPP-4 inhibitors augment the effects of incretin hormones by prolonging their half-life and represent a new therapeutic approach for the treatment of type 2 diabetes
More informationInnate Immunity. Hathairat Thananchai, DPhil Department of Microbiology Faculty of Medicine Chiang Mai University 2 August 2016
Innate Immunity Hathairat Thananchai, DPhil Department of Microbiology Faculty of Medicine Chiang Mai University 2 August 2016 Objectives: Explain how innate immune system recognizes foreign substances
More informationPublished on Second Faculty of Medicine, Charles University (http://www.lf2.cuni.cz )
Published on Second Faculty of Medicine, Charles University (http://www.lf2.cuni.cz ) Biochemistry Submitted by Marie Havlová on 8. February 2012-0:00 Syllabus of Biochemistry Mechanisms of enzyme catalysis.
More informationQUIZ/TEST REVIEW NOTES SECTION 7 NEUROPHYSIOLOGY [THE SYNAPSE AND PHARMACOLOGY]
QUIZ/TEST REVIEW NOTES SECTION 7 NEUROPHYSIOLOGY [THE SYNAPSE AND PHARMACOLOGY] Learning Objectives: Explain how neurons communicate stimulus intensity Explain how action potentials are conducted along
More informationFundamentals of the Nervous System and Nervous Tissue: Part C
PowerPoint Lecture Slides prepared by Janice Meeking, Mount Royal College C H A P T E R 11 Fundamentals of the Nervous System and Nervous Tissue: Part C Warm Up What is a neurotransmitter? What is the
More informationType 1 Diabetes 2/23/2015. Endocrine System Hormones. Living with Type 1 Diabetes
Endocrine System Hormones 2007-2008 Living with Type 1 Diabetes Type 1 Diabetes results from the autoimmune destruction of the insulin- producing beta-cells in the pancreas. The lack of insulin leads to
More informationPhysiology Unit 1 CELL SIGNALING: CHEMICAL MESSENGERS AND SIGNAL TRANSDUCTION PATHWAYS
Physiology Unit 1 CELL SIGNALING: CHEMICAL MESSENGERS AND SIGNAL TRANSDUCTION PATHWAYS In Physiology Today Cell Communication Homeostatic mechanisms maintain a normal balance of the body s internal environment
More informationRAISON D ETRE OF THE IMMUNE SYSTEM:
RAISON D ETRE OF THE IMMUNE SYSTEM: To Distinguish Self from Non-Self Thereby Protecting Us From Our Hostile Environment. Innate Immunity Acquired Immunity Innate immunity: (Antigen nonspecific) defense
More informationThe T cell receptor for MHC-associated peptide antigens
1 The T cell receptor for MHC-associated peptide antigens T lymphocytes have a dual specificity: they recognize polymporphic residues of self MHC molecules, and they also recognize residues of peptide
More informationAllergy and Immunology Review Corner: Chapter 13 of Immunology IV: Clinical Applications in Health and Disease, by Joseph A. Bellanti, MD.
Allergy and Immunology Review Corner: Chapter 13 of Immunology IV: Clinical Applications in Health and Disease, by Joseph A. Bellanti, MD. Chapter 13: Mechanisms of Immunity to Viral Disease Prepared by
More informationProteins are sometimes only produced in one cell type or cell compartment (brain has 15,000 expressed proteins, gut has 2,000).
Lecture 2: Principles of Protein Structure: Amino Acids Why study proteins? Proteins underpin every aspect of biological activity and therefore are targets for drug design and medicinal therapy, and in
More information18. PANCREATIC FUNCTION AND METABOLISM. Pancreatic secretions ISLETS OF LANGERHANS. Insulin
18. PANCREATIC FUNCTION AND METABOLISM ISLETS OF LANGERHANS Some pancreatic functions have already been discussed in the digestion section. In this one, the emphasis will be placed on the endocrine function
More informationClose to site of release (at synapse); binds to receptors in
Chapter 18: The Endocrine System Chemical Messengers 1. Neural 2. Endocrine 3. Neuroendocrine 4. Paracrine 5. Autocrine Endocrine System --Endocrine and nervous systems work together --Endocrine vs. Nervous
More informationImmunology lecture: 14. Cytokines: Main source: Fibroblast, but actually it can be produced by other types of cells
Immunology lecture: 14 Cytokines: 1)Interferons"IFN" : 2 types Type 1 : IFN-Alpha : Main source: Macrophages IFN-Beta: Main source: Fibroblast, but actually it can be produced by other types of cells **There
More informationComprehensive and Easy Course Notes for BIOL1040 Exams and Assessment
Comprehensive and Easy Course Notes for BIOL1040 Exams and Assessment MODULE 1: PRINCIPLES OF CELL FUNCTION Membrane Structure & Function Cellular membranes are fluid mosaics of lipids and proteins Phospholipids
More informationMolecular Cell Biology - Problem Drill 19: Cell Signaling Pathways and Gene Expression
Molecular Cell Biology - Problem Drill 19: Cell Signaling Pathways and Gene Expression Question No. 1 of 10 1. Which statement about cell signaling is correct? Question #1 (A) Cell signaling involves receiving
More informationPlasma membranes. Plasmodesmata between plant cells. Gap junctions between animal cells Cell junctions. Cell-cell recognition
Cell Communication Cell Signaling Cell-to-cell communication is essential for multicellular organisms Communicate by chemical messengers Animal and plant cells have cell junctions that directly connect
More informationimmunity produced by an encounter with an antigen; provides immunologic memory. active immunity clumping of (foreign) cells; induced by crosslinking
active immunity agglutination allografts immunity produced by an encounter with an antigen; provides immunologic memory. clumping of (foreign) cells; induced by crosslinking of antigenantibody complexes.
More informationENERGY FROM INGESTED NUTREINTS MAY BE USED IMMEDIATELY OR STORED
QUIZ/TEST REVIEW NOTES SECTION 1 SHORT TERM METABOLISM [METABOLISM] Learning Objectives: Identify primary energy stores of the body Differentiate the metabolic processes of the fed and fasted states Explain
More informationInnate Immunity. Connection Between Innate and Adaptive Immunity. Know Differences and Provide Examples Chapter 3. Antimicrobial peptide psoriasin
Know Differences and Provide Examples Chapter * Innate Immunity * kin and Epithelial Barriers * Antimicrobial peptide psoriasin -Activity against Gram (-) E. coli Connection Between Innate and Adaptive
More informationM.Sc. III Semester Biotechnology End Semester Examination, 2013 Model Answer LBTM: 302 Advanced Immunology
Code : AS-2246 M.Sc. III Semester Biotechnology End Semester Examination, 2013 Model Answer LBTM: 302 Advanced Immunology A. Select one correct option for each of the following questions:- 2X10=10 1. (b)
More informationWhat are the 6 types of neuroglia and their functions?!
Warm Up! Take out your 11C Notes What are the 6 types of neuroglia and their functions?! Astrocytes Microglia Ependymal Cells Satellite Cells Schwann Cells Oligodendrocytes Support, brace, & nutrient transfer
More informationMolecular Cell Biology Problem Drill 16: Intracellular Compartment and Protein Sorting
Molecular Cell Biology Problem Drill 16: Intracellular Compartment and Protein Sorting Question No. 1 of 10 Question 1. Which of the following statements about the nucleus is correct? Question #01 A. The
More informationSolution key Problem Set
Solution key- 7.013 Problem Set 6-2013 Question 1 a) Our immune system is comprised of different cell types. Complete the table below by selecting all correct cell types from the choices provided. Cells
More informationGENERAL CHARACTERISTICS OF THE ENDOCRINE SYSTEM FIGURE 17.1
GENERAL CHARACTERISTICS OF THE ENDOCRINE SYSTEM FIGURE 17.1 1. The endocrine system consists of glands that secrete chemical signals, called hormones, into the blood. In addition, other organs and cells
More informationSarah Jaar Marah Al-Darawsheh
22 Sarah Jaar Marah Al-Darawsheh Faisal Mohammad Receptors can be membrane proteins (for water-soluble hormones/ligands) or intracellular (found in the cytosol or nucleus and bind to DNA, for lipid-soluble
More information