Nerve Muscle Relationship and Neural Muscular Junction Quiz. Remember, you need to know the structure and the function!
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1 Nerve Muscle Relationship and Neural Muscular Junction Quiz Remember, you need to know the structure and the function!
2 What is this called? What is this? Schwann cell What is this called? Basal lamina What do these vesicles contain? What is this? What is this space called? Nucleus What are these structures? Junctional folds Nucleus Sarcoplasm Mitochondria What is this?
3 The Neuromuscular Junction Motor nerve fiber Myelin Schwann cell Synaptic knob Basal lamina Synaptic vesicles (containing ACh) Sarcolemma Synaptic cleft Nucleus ACh receptor Junctional folds Nucleus Sarcoplasm Mitochondria Myofilaments
4 What is this step called? (steps 1 and 2) Nerve signal Motor nerve fiber Sarcolemma What happens here as nerve signal reaches this point? Synaptic knob What s inside these vesicles? What are these structures? Synaptic cleft 1 2 What happens at #1? What happens at #2?
5 Excitation (steps 1 and 2) Nerve signal Motor nerve fiber Sarcolemma enters synaptic knob Synaptic knob Synaptic vesicles ACh ACh receptors Synaptic cleft 1 Arrival of nerve signal 2 Acetylcholine (ACh) release nerve signal opens voltage regulated calcium gated channels in synaptic knob calcium stimulates exocytosis of ACh from synaptic vesicles ACh released into synaptic cleft
6 This is part of what process? (steps 3 and 4) ACh ACh ACh receptor K + Sarcolemma Na + 3 What just happened here? 4 What is the term that describes this type of potential? What is the form of regulation (type) at these gates?
7 Excitation (steps 3 and 4) ACh ACh ACh receptor K + Sarcolemma Na + 3 Binding of ACh to receptor 4 Opening of ligand-regulated ion gate; creation of end plate potential two ACh molecules bind to each receptor protein, opens ligand regulated Na + and K + channels. (i.e. Ach is the ligand) Na + enters shifting RMP /// goes from -90mV to +75mV /// then K + exits and RMP returns to -90mV /// quick voltage shift is called the end-plate potential (EPP) // a type of action potential
8 This is part of what process? (step 5) K + Plasma membrane of synaptic knob Na + What type of regulated ion gates? Sarcolemma 5 What is being created just outside of the neuromuscular endplate?
9 Excitation (step 5) K + Plasma membrane of synaptic knob Na + Voltage-regulated ion gates Sarcolemma 5 Opening of voltage-regulated ion gates; creation of action potentials Voltage change (in end-plate region) spreads to nearby voltage regulated gated Na and K channels to produce an action potential just outside of the neuromuscular junction that then spreads over entire muscle surface.
10 What is this step called? (steps 6 and 7) 8 What is this? What is this? What is this? 6 7 #6) What term describes the movement of the yellow arrows? #7) What event is occurring? Is it active or passive? #8) What do these three structures form?
11 Excitation-Contraction Coupling (steps 6 and 7) Terminal cisterna of SR T tubule T tubule Sarcoplasmic reticulum 6 Action potentials propagated 7 Calcium released from down T tubules terminal cisternae action potential (AP) spreads from sarcoplasm into T tubules AP flows from T tubules to sarcoplasmic reticulum AP opens voltage regulated gated calcium ion channels in SR Ca +2 diffuse into the cytosol
12 This is part of what process? (steps 8 and 9) What is this? What is this? What is this? What is this? What is this? 8 Where did the Calcium come from? 9 What just happened here? What was the method of regulation?
13 Excitation-Contraction Coupling (steps 8 and 9) TroponinTropomyosin Actin Thin filament Active sites Myosin 8 Binding of calcium to troponin 9 Shifting of tropomyosin; exposure of active sites on actin calcium binds to troponin in thin filaments troponin-tropomyosin complex changes shape and exposes active sites on actin this is the site where the energized myosin head must bind to in order for a muscle to shorten
14 What is this step called? (steps 10 and 11) Troponin Tropomyosin What happened to the ATP? ADP P i Myosin What happened to the myosin head? 10 Actin Myosin 11 What do we call the attachment of the myosin head to actin? What happens to the myosin head after it attaches to the actin binding site?
15 Contraction (steps 10 and 11) myosin ATPase enzyme in myosin head hydrolyzes ATP molecule Troponin Tropomyosin This reaction occurs independent of the actin troponin tropomyocin event 10 ADP P i Myosin Hydrolysis of ATP to ADP + Pi; activation and cocking of myosin head Myosin head is activated = the head cocks to extends head /// ADP + P i remain attached to head Cross-bridge: Actin Myosin head binds to actin active site forming a myosin - actin cross-bridge 11 Formation of myosin actin cross-bridge Now ADP + P released from the myosin head
16 This is part of what process? (steps 12 and 13) ATP 13 What caused the myosin head to release the actin binding site? ADP P i 12 What phrase describes this event? What is released from the myosin head as it binds to actin?
17 The Power Stroke Myosin head flexes Contraction (steps 12 and 13) Pulls thin filament over thick filament // Z disc move closer together Myosin head can not release actin until new ATP molecule binds to myosin // this breaks the bridge Only upon binding more ATP to the myosin head myosin releases actin and the process is repeated ATP 13 Binding of new ATP; breaking of cross-bridge each head performs 5 power strokes per second each stroke utilizes one molecule of ATP ADP P ii 12 Power stroke; sliding of thin filament over thick filament As one bridge is broken many more are formed which maintains tension in muscle
18 What is this step called? (steps 14 and 15) ACh AChE 14 What happens when the nerve s action potential stops? 15 What is the function of acetylcholesterase?
19 Relaxation (steps 14 and 15) AChE ACh 14 Cessation of nervous stimulation and ACh release 15 ACh breakdown by acetylcholinesterase (AChE) Stop nerve stimulation to stop ACh release AChE breaks down Ach // fragments reabsorbed into synaptic knob This stops stimulation by ACh // reverses all downstream events
20 This is part of what process? (step 16) Terminal cisterna of SR 16 What is the action of the calcium ions? What is it called? Is it active or passive?
21 Ca +2 pumped back into SR by active transport. // this is active transport (Why?) Ca +2 binds to calsequestrin while in storage in SR Relaxation (step 16) Terminal cisterna of SR ATP is needed for muscle relaxation as well as muscle contraction. 16 Reabsorption of calcium ions by sarcoplasmic reticulum
22 This is part of what process? (steps 17 and 18) What happens when calcium is releases from tropinin? 17 ADP P i Tropomyosin ATP 18 Where did the tropomyosin move?
23 Relaxation (steps 17 and 18) Ca +2 removed from troponin is pumped back into SR tropomyosin blocks once again the myosin binding sites ADP P i muscle fiber ceases to produce or maintain tension 17 Loss of calcium ions from troponin muscle fiber returns to its resting length due to recoil of elastic components & contraction of antagonistic muscles Tropomyosin ATP 18 Return of tropomyosin to position blocking active sites of actin
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