optical mapping analysis Tyrode connexin43 Sprague Dawley male 8 weeks old g n 64 ketamine hydrochloride 5 mg/kg

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1 14 Symposium 39 optical mapping SD Tyrode Na channel di 4 ANEPPS action potential duration dispersion APDd VTVF APDd VT/VF 30 1,2 3 optical mapping analysis connexin43 1 Sprague Dawley male 8 weeks old g n 64ketamine hydrochloride 5 mg/kg n 41 1 PhysioTel and DataquestTM, Data Sciences International, Bonpei Takase, et al. Pneumonectomy induced transient right ventricular pressure overload causes vulnerability to lethal arrhythmias 1616

2 Symposium St Paul, Minnesota, USA 2 8 MHz UF 750XT, Fukuda Denshi Co Ltd., Tokyo, Japan RV LV inter ventricular septum IVS RV/ LV IVS 3 optical mapping analysis 37 Tyrode CaCl2 [2], NaCl [140], KCl [4.5], dextrose [10], MgCl2 [1], and HEPES [10, ph 7.4], in mmol/l Tyrode Na di 4 ANEPPS [15 mol/ L] 40mL butanedione monoxime Wako Chemical, Tokyo, Japan, 20 mm optical mapping analysis high quality charge couple device CCD camera Leica , Geneva, Switzerland4 APD commercialized software Ultima 6006; Sei Media, Inc., Tokyo, Japan 5 5mm APD APD APD APD 60ms APD APD APD dispersion APD dispersion 3 20 burst pacing, 5, 50, 100V 40 ms interval, 20trains voltage paraformaldehyde phosphate buffer solution Wako Pure Chemical Industries Ltd ethanolparaffin hematoxylin and eosin, Elastica van Gieson Masson trichrome connexin43 Anti connexin43 monoclonal antibody 1 2,000, Sigma Aldrich, St. Louis, USA gap junction connexin APD dispersion 4 2 APD dispersion burst pacing 1617

3 16 Symposium [APD] ADP dispersion 1618

4 Symposium burst pacing A 1 100v 20 trains at RV outflow B 4 100v 20 trains at RV outflow 1619

5 18 Symposium 39 4 connexin43 1 Day 1 n 7 Day 4 n 7 1 week n 7 2 weeks n 20 4 weeks n 10 srvp mmhg RAP mmhg RV dilatation RV conduction disturbance VF/VT induction 3 APD dispersion connexin APD dispersion APD dispersion connexin

6 Symposium APD dispersion 3 4 connexin43 4, 5 APD dispersion stretch activated ion channel SAC APD connexin43 SAC APD dispersion 6, 7 SAC tarantula venom peptide GsMTx 4 1 Rubin LJ. Introduction: Diagnosis and management of pulmonar y arterial hypertension: ACCP evidence based clinical practice guideline. Chest 2004;126:7S 10S. 2 Kuriyama T. Clinical aspect of precapillary pulmonary hypertension, Nihon Kyobu Sikkan Gakkai Zasshi JP J Respir Disease 1992;30: Umar S, Lee JH, da Lange E, Iorga A, Partow Navid R, Bapat A, et al. Spontaneous ventricular fibrillation in right ventricular failure secondary to chronic pulmonary hypertension. Circ Arrhythm Electrophysiol 2012;5: Koura T, Hara M, Takeuchi S, Ota K, Okada Y, Miyoshi S, et al. Anisotropic conduction properties in canine atria analyzed by high resolution optical mapping: preferential direction of conduction block changes from longitudinal to transverse with increasing age. Circulation 2002;105: Sasano C, Honjo H, Takagishi Y, Uzzaman M, Emdad L, Shimizu A, et al. Internalization and dephosphorylation of connexin43 in hypertrophied right ventricles of rats with pulmonary hypertension. Circ J 2007;71: Kohl P, Bollensdorff C, Garny A. Effects of mechanosensitive ion channels on ventricular electrophysiology: experimental and theoretical models. Exp Physiol 2006;91: Morris CE, Juranka PF. Nav channel mechanosensitivity: activation and inactivation accelerate reversibly with stretch. Biophys J 2007;93: