INTERACTION OF SEVERAL TRADITIONAL MEDICINE PREPARATIONS WITH AMINOPYRINE IN RAT HEPATOCYTES

Size: px
Start display at page:

Download "INTERACTION OF SEVERAL TRADITIONAL MEDICINE PREPARATIONS WITH AMINOPYRINE IN RAT HEPATOCYTES"

Transcription

1 INTERACTION OF SEVERAL TRADITIONAL MEDICINE PREPARATIONS WITH AMINOPYRINE IN RAT HEPATOCYTES H.H. Abas, H.B. Saringat and A.A.Rozana The study looks at the in vitro interaction of three Malay (Air Gamat Asli Langkawi, Faizol Tonik and Air Jamu Pak Tani ) and three Chinese (Yomeishu, Ginseng & Polygona Root Extractum and King To Nin Jiom Pei Pa Kao ) traditional medicine preparations aminopyrine in rat liver hepatocytes. Aminopyrine N-demethylase activity was determined by measuring the quantity of formaldehyde formed. Results indicate that Faizol Tonik a and Air Jamu Pak Tamu increase the activity of aminopyrine N-demethylase significantly (P<0.05) whereas Air Gamat Asli Langkawi produces no significant change in the enzymatic activity when compared to control. With the Chinese traditional medicine preparations, all three preparations investigated increase the activity of aminopyrine N-demethylase significantly (P<0.05). Thus, several of the traditional medicine preparations tested were found to affect the metabolism of aminopyrine in-vitro and whether the biovailability of aminopyrine in-vivo can be similarly affected by these preparations remains to be investigated INTRODUCTION Systematic botanical, chemical and pharmacological studies of medicinal plants are being actively conducted in many universities and research institutions in Malaysia. Major portions of research are being conducted on crude plant extracts, soluble plant fractions and pure compounds. Literature search indicated that there are still limited research done on traditional medicine preparations that are available in the market. Most traditional medicine preparations consumed by the public, irrespective of racial origin, usually contain more than one plant extracts as their active ingredients. The therapeutic outcome or adverse effect/s of these preparations thus could have been derived from the summation of all the ingredients in the traditonal medicine preparations. The analysis of these preparations available in the market is of more immediate relevance with respect to the health benefit or risk to consumer. Furthermore, such information is much needed by the relevant government authorities responsible in overseing the registration of all traditional medicine preparations as required under the National Drug Control Legislation. This preliminary study aims to investigate possible in vitro interactions between three Malay and three Chinese traditional medicine preparations available in the market with aminopyrine utilising the rat liver hepatocytes. Article V, May 1998 Page 1

2 Interaction Of Traditional Medicine Preparations With Aminopyrine In Rat Hepatocytes ARBEC, May 1998 MATERIAL AND METHODS Chemicals Used All chemicals used were of analytical grade purity. Collagenase (type IV) and aminopyrine were obtained from the Sigma Chemical Co., St. Louis, MO, USA. The medicinal preparations were obtained from local medicine shops. Air Gamat Asli Langkawi was manufactured by Keluaran Gamat Asli Langkawi, Langkawi, Kedah; Faizol Tonik was manufactured by Kilang Faizol Tonik, Kuala Kangsar, Perak and Air Jamu Pak Tani was manufactured by Perusahaan Jamu Arjuna, Batu Pahat, Johor; Yomeishu was manufactured by Yomeishu Seizo Co. Ltd., Tokyo, Japan; Ginseng & Polygona Root Extractum was manufactured by Tianjin Medicine and Health Products, China and King To Nin Jiom Pei Pa Kao was manufactured by Nin Jiom Medicine Manufacturing (Hong Kong) Limited. Preparation of Hepatocytes and Liver Enzyme Assay Forty to sixty-weeks old normal male rats of the Sprague Dawley strain, bred in the School of Pharmaceutical Sciences, Universiti Sains Malaysia were used. All rats weighing between g were fed a normal laboratory chow (Gold Coin ) ad libitum and had free access to tap water. Isolated hepatocytes were prepared by using the modified collagenase perfusion technique (Hussin and Skett, 1988). The animals were anaesthetised with ether and the liver perfused in-situ through the hepatic portal vein with calcium-free Hank's Balanced Salt Solution (HBSS) for about 15 minutes followed by the collagenase buffer (HBSS supplemented with 4 mm calcium chloride and collagenase 0.5 mg/ml) until the liver appeared to have broken up. The liver was removed from the animal and the cells dispersed in the calcium-free HBSS using a steel forcep. The cell suspension was filtered through gauze and centrifuged at 200g for 5 minutes (in a Hettich Universal centrifuge) to clean the parenchymal cells of debris. The supernatant was removed and cells suspended in incubation medium (HBSS supplemented with lg/l glucose, 100mg/L MgS0 4, 100mg/L MgC1 2 and 185mg/L CaCl 2 ; ph 7.4). The cells were then counted using a hemocytometer and assayed for viability using trypan blue exclusion method. Average yield was about 5 x 10 7 cells/g liver and viability was routinely greater than 90 % for all preparations. Equal volume (0.5 ml) of serial dilutions of the traditional medicine preparations were added to petri dishes containing aminopyrine (25nM final concentration), 2 x 10 6 hepatocytes and incubation medium (final volume of 10 ml). Control petri dishes contain the same but had the traditional medicine preparations replaced with distilled water. The highest volume of the traditional medicine preparations was chosen so as not to interfere with spectrophotometric measurement. The petri dishes were then incubated in C0 2 incubator (Queue Systems Inc./Model 2710) for 18 minutes at 37 C. Aminopyrine N-demethylase activity was determined by measuring the quantity of formaldehyde formed according to the colorimetric method of Nash (1953). Statistical Analysis Results were presented as means ± S.D. Statistical test within groups were analysed using Student t-test from a computerised Statswork software. Article V, May 1998 Page 2

3 RESULTS AND DISCUSSION The pharmacological or adverse effect/s that resulted from the consumption of any traditional medicine preparations is derived from the combination of effects of the total ingredients in a preparation. This is well differentiated from the consumption of allopathic medicine whereupon the content and dose of an active ingredient in any particular dosage form is well quantitated due to more stringent quality control. The traditional medicine preparations available in the market usually contain more than one plant extracts or other biological sources. Figure 1 indicates that at dilutions higher than 1:8, Air Jamu Pak Tani significantly increase the activity of aminopyrine N-demethylase in the rat liver. Undiluted volume of the same preparation exerted a 90 % activity increase above control (P < 0.001) whereas at lower dilutions (< 1: 16) no significant effect (P > 0.05) was produced. It is not known however which of the I I natural plant extracts used in the concoction of Jamu Pak Tani is the active ingredient producing the effect observed. Air Gamat Asli Langkawi, a single component concoction, showed no significant effect on aminopyrine N-demethylase activity when compared to control (Figure 2). Compared to control, serial dilutions of this preparation exerted no significant influence on the liver enzyme activity. Faizol Tonik exerted significant increase in aminopyrine Ndemethylase activity (Figure 3). At one-eighth and one-fourth dilutions, the enzyme activity was increased by about 16 % above control (P < 0.05); at half dilution, 41% above control (P < 0.001) and undiluted preparation increased the enzyme activity by 96 % above control (P < 0.001). King To Nin Jiom Pei Pa Kao also increased aminopyrine Ndennethylase activity (Figure 4). Undiluted preparation exerted a 61% increase in enzyme activity when compared to control (P < 0.001) whereas one-eighth and higher dilutions had no effect on the enzyme activity. Undiluted Yomeishu significantly increased aminopyrine Ndemethylase activity by 92 % (P < 0.001). The enzyme activity significantly increased with decreasing dilutions of Yomeishu and dilutions of one-sixteenth and higher exerted no significant effect on the enzyme activity with-respect to control (Figure 5). Only half dilution and undiluted Ginseng & Polygona Root Extractum exerted significant increase in aminopyrine N-demethylase activity (34 % and 91% respectively) (Figure 6). One-fourth and higher dilutions showed no significant effect on the enzyme activity when compared to control. With the exception of Air Gamat Asli Langkawi all the other five preparations investigated in this study have more than one plant source. As documented on the bottle container, Air Jamu Pak Tani and Faizol Tonik have I I and 17 ingredients natural sources respectively; Yomeishu, King To Nin Jioin Pei Pa Kao and Ginseng & Polygona Root Extractum have 14, 16 and 2 herbal ingredients respectively. Article V, May 1998 Page 3

4 Interaction Of Traditional Medicine Preparations With Aminopyrine In Rat Hepatocytes ARBEC, May 1998 Figure 1 : In-vitro effect of Air Jamu Pak Tani an aminopyrine N-demethylase activity in normal male rat hepatocytes Figure 2 - In-vitro effect of Air Gamat Asli Langkawi on aminopyrine Article V, May 1998 Page 4

5 Figure 3 - In-vitro effect of Faizol Tonik on aminopyrine N-demethylase activity in normal male rat hepatocytes Figure 4 - In-vitro effect of King To Nin Jiam Pei Pa Kao on aminopyrine Article V, May 1998 Page 5

6 Interaction Of Traditional Medicine Preparations With Aminopyrine In Rat Hepatocytes ARBEC, May 1998 Figure 5 - In-vitro effect of Yomeishu on aminopyrine Figure 6 - In-vitro effect of Ginseng and Polygona root extractum on aminopyrine Article V, May 1998 Page 6

7 Aminopyrine undergoes phase I N-demethylation process in the liver in the presence of cytochrome P-450 N-demethylase. The increase in the aminopyrine N-dernethylase activity in-vitro as illustrated in this study may increase the metabolism of aminopyrine and tentatively reduces the latter bioavailability. Other clinical drugs such as tricyclic antidepressants such as imipramine, desipramine; neuroleptic drugs such as phenothiazine, chlorpromazine and thioridazine; procainamide, cocaine, morphine and salicylamide are also known to undergo N-- demethylation in-vivo. Thus there is also a possibility that the traditional medicine preparations investigated in this study may increase the metabolism of these clinical drugs. However, it should be stressed that the effect here is only seen in-vitro and a similar effect in-vivo needs to be investigated. In conclusion, the study indicated that all the traditional medicine preparations investigated (except Air Garnat Asli Langkawi ) significantly increase aminopyrine N-demethylase activity in rat hepa-tocytes in-vitro. A possibility exists that similar interactions may occur in-vitro with other drugs that undergo hepatic phase I N-demethylation. Whether this effect is similarly produced in-vivo still needs further investigation. ACKNOWLEDGEMENT The authors acknowledge the research grant No. 131/0210/0520 provided by the Universiti Sains Malaysia, Penang, for making this study possible. REFERENCES Hussin, A.H. and Skett, P Lack of effect of insulin in hepatocytes isolated from streptozotocin-diabetic male rats. Biochem. Pharmac. 37: Nash, T The colorimetric estimation of formaldehyde by means of the Hantzsch Reaction. Biochem. J, 55: Article V, May 1998 Page 7

Pharmacologyonline 3: (2010)

Pharmacologyonline 3: (2010) EFFECT OF ORTHOSIPHO STAMI EUS A D TI OSPORA CRISPA O AMI OPYRI E METABOLISM I RATS Jin Han Chin, 1* Phaik Tin Tan 2, Abas Hj. Hussin 3 1 Faculty of Pharmaceutical Sciences, UCSI University, No. 1, Jalan

More information

EFFECT OF THE ORTHOSIPHON STAMINEUS, BENTH ON AMINOPYRINE METABOLISM IN RAT HEPATOCYTES

EFFECT OF THE ORTHOSIPHON STAMINEUS, BENTH ON AMINOPYRINE METABOLISM IN RAT HEPATOCYTES Malaysian Journal of Pharmaceutical Sciences, Vol. 5, No. 1, 25 32 (2007) EFFECT OF THE ORTHOSIPHON STAMINEUS, BENTH ON AMINOPYRINE METABOLISM IN RAT HEPATOCYTES CHIN JIN HAN 1* AND ABAS HJ. HUSSIN 2 1School

More information

Recombinant Trypsin, Animal Origin Free

Recombinant Trypsin, Animal Origin Free Recombinant Trypsin, Animal Origin Free PRODUCT INFORMATION: BioGenomics r-trypsin powder is ready to use, animal origin free optimized for cell culture applications. It is derived by r-dna technology.

More information

METABOLISM OF DRUGS BY SUBFRACTIONS OF HEPATIC MICROSOMES FROM PROLONGED ETHANOL-TREATED RATS

METABOLISM OF DRUGS BY SUBFRACTIONS OF HEPATIC MICROSOMES FROM PROLONGED ETHANOL-TREATED RATS METABOLISM OF DRUGS BY SUBFRACTIONS OF HEPATIC MICROSOMES FROM PROLONGED ETHANOL-TREATED RATS Suehiro NAKANISHI, Go KINOSHITA, Eiko SHIOHARA and Miyoko TSUKADA Department of Pharmacology, Faculty of Medicine,

More information

CANINE CARDIAC MYOCYTE ISOLATION PROTOCOL November 2000

CANINE CARDIAC MYOCYTE ISOLATION PROTOCOL November 2000 CANINE CARDIAC MYOCYTE ISOLATION PROTOCOL November 2000 I. Preparation for cell isolation: A. Make sure the following items are autoclaved: 1. 1-8X8 baking dish 6. 3-1 liter bottles 2. 2-500 ml beaker

More information

DIASTASE ACTIVITY IN HONEY ASSAY PROCEDURE K-AMZHY 04/05

DIASTASE ACTIVITY IN HONEY ASSAY PROCEDURE K-AMZHY 04/05 DIASTASE ACTIVITY (α-amylase) IN HONEY ASSAY PROCEDURE K-AMZHY 04/05 Megazyme International Ireland Limited 2004 INTRODUCTION: The traditional method for the measurement of diastase activity in honey isthe

More information

Hassan Pyar Kok-Khiang Peh *

Hassan Pyar Kok-Khiang Peh * Isolation of probiotics Lactobacillus acidophilus from commercial yoghurt Hassan Pyar Kok-Khiang Peh * School of Pharmaceutical Sciences, University Sains Malaysia, 11800 Minden, Penang, Malaysia. Telephone

More information

4. Determination of fat content (AOAC, 2000) Reagents

4. Determination of fat content (AOAC, 2000) Reagents 94 ANALYTICAL METHODS 1. Determination of moisture content (AOAC, 2000) 1. Dry the empty dish and lid in the oven at 105 C for 3 h and transfer to desiccator to cool. Weigh the empty dish and lid. 2. Weigh

More information

Protocol for Thawing Cryopreserved Hepatocytes

Protocol for Thawing Cryopreserved Hepatocytes cell and tissue-based products Protocol for Thawing Cryopreserved Hepatocytes Product Instruction The following procedure may be carried out in a biosafety containment hood to reduce the risk of contamination

More information

FABRICATION AND EVALUATION OF GLIMEPIRIDE CORDIA DICHOTOMA G.FORST FRUIT MUCILAGE SUSTAINED RELEASE MATRIX TABLETS

FABRICATION AND EVALUATION OF GLIMEPIRIDE CORDIA DICHOTOMA G.FORST FRUIT MUCILAGE SUSTAINED RELEASE MATRIX TABLETS Int. J. Chem. Sci.: 7(4), 2009, 2555-2560 FABRICATION AND EVALUATION OF GLIMEPIRIDE CORDIA DICHOTOMA G.FORST FRUIT MUCILAGE SUSTAINED RELEASE MATRIX TABLETS HINDUSTAN ABDUL AHAD *, B. PRADEEP KUMAR, C.

More information

Tongkat Ali Extract / Longjack Extract

Tongkat Ali Extract / Longjack Extract Tongkat Ali Extract Tongkat Ali Extract / Longjack Extract Tongkat Ali or Longjack extract also known as Eurycoma Longifolia - 100:1 and 200:1 root extracts are a concentrated form of the soluble parts

More information

IN VITRO ANTICANCER ACTIVITY OF FLOWER EXTRACTS OF COUROUPITA GUIANENSIS

IN VITRO ANTICANCER ACTIVITY OF FLOWER EXTRACTS OF COUROUPITA GUIANENSIS CHAPTER 3 IN VITRO ANTICANCER ACTIVITY OF FLOWER EXTRACTS OF COUROUPITA GUIANENSIS 3. INTRODUCTION Plants are the basic source of knowledge of modern medicine. Almost all the parts of the plant, namely

More information

TOXICOLOGY PROTOCOLS PROTOCOL 1. SERIAL DILUTIONS. Objective To make a serial dilution for use in dose/response bioassays.

TOXICOLOGY PROTOCOLS PROTOCOL 1. SERIAL DILUTIONS. Objective To make a serial dilution for use in dose/response bioassays. TOXICOLOGY PROTOCOLS PROTOCOL 1. SERIAL DILUTIONS Objective To make a serial dilution for use in dose/response bioassays. Background The idea behind a bioassay is that the test organism will respond in

More information

L. E. Phillip, M.V. Simpson, E. S. Idziak H and S.F. Kubow*

L. E. Phillip, M.V. Simpson, E. S. Idziak H and S.F. Kubow* Ruminal and metabolic effects of pure lignin in sheep fed low and high fibre diets. L. E. Phillip, M.V. Simpson, E. S. Idziak H and S.F. Kubow* Introduction Previous studies with cattle indicated that

More information

Similarity & Difference in OTC Classification and their Borderlines among Asia Pacific

Similarity & Difference in OTC Classification and their Borderlines among Asia Pacific Similarity & Difference in OTC Classification and their Borderlines among Asia Pacific Dr. Noppadon (Nhum) Adjimatera South East Asia (SEA) Regulatory & Medical Affairs Director, RB, SEA, Thailand 22 October

More information

CytoPainter Lysosomal Staining Kit - Blue Fluorescence

CytoPainter Lysosomal Staining Kit - Blue Fluorescence ab112135 CytoPainter Lysosomal Staining Kit - Blue Fluorescence Instructions for Use For staining Lysosomes in suspension and adherent cells by using our proprietary blue fluorescence probe This product

More information

Relaxation responses of aortic rings from salt-loaded high calcium fed rats to potassium chloride, calcium chloride and magnesium sulphate

Relaxation responses of aortic rings from salt-loaded high calcium fed rats to potassium chloride, calcium chloride and magnesium sulphate Pathophysiology 4 (1998) 275 280 Relaxation responses of aortic rings from salt-loaded high calcium fed rats to potassium chloride, calcium chloride and magnesium sulphate B.J. Adegunloye, O.A. Sofola

More information

Appendix A: Preparation of Media and Chemicals. Malt Extract Agar (MEA) weighing g was dissolved in 400 ml of distilled water

Appendix A: Preparation of Media and Chemicals. Malt Extract Agar (MEA) weighing g was dissolved in 400 ml of distilled water Appendix A: Preparation of Media and Chemicals Preparation of Malt Extract Agar (MEA) Malt Extract Agar (MEA) weighing 13.44 g was dissolved in 400 ml of distilled water in an Erlenmeyer flask using a

More information

INSTRUCTIONS Pierce Primary Cardiomyocyte Isolation Kit

INSTRUCTIONS Pierce Primary Cardiomyocyte Isolation Kit INSTRUCTIONS Pierce Primary Cardiomyocyte Isolation Kit 88281 Number Description 88281 Pierce Primary Cardiomyocyte Isolation Kit, contains sufficient reagents to isolate cardiomyocytes from 50 neonatal

More information

2017, Pharmapex USA, A Member of Apex Group of Companies

2017, Pharmapex USA, A Member of Apex Group of Companies 2017, Pharmapex USA, A Member of Apex Group of Companies Natural Blood Health Aid uses a proprietary and licensed Virgin Isolate Technology, invented which recreates the Ayurvedic method used in ancient

More information

CytoPainter LysoGreen Indicator Reagent

CytoPainter LysoGreen Indicator Reagent ab176826 CytoPainter LysoGreen Indicator Reagent Instructions for Use For staining lysosomes in live cells with our proprietary Green probe. This product is for research use only and is not intended for

More information

Modulating Glucose Uptake in Skeletal Myotubes: Insulin Induction with Bioluminescent Glucose Uptake Analysis

Modulating Glucose Uptake in Skeletal Myotubes: Insulin Induction with Bioluminescent Glucose Uptake Analysis icell Skeletal Myoblasts Application Protocol Modulating Glucose Uptake in Skeletal Myotubes: Insulin Induction with Bioluminescent Glucose Uptake Analysis Introduction The skeletal muscle is one of the

More information

Research Article GALLIC ACID AND FLAVONOID ACTIVITIES OF AMARANTHUS GANGETICUS

Research Article GALLIC ACID AND FLAVONOID ACTIVITIES OF AMARANTHUS GANGETICUS ISSN 2395-3411 Available online at www.ijpacr.com 238 Research Article GALLIC ACID AND FLAVONOID ACTIVITIES OF AMARANTHUS GANGETICUS G. Jyoti Jain 1* and S. Ramachandra Setty 2 1 Department of Pharmacology,

More information

Rat cholesterol ELISA Kit

Rat cholesterol ELISA Kit Rat cholesterol ELISA Kit Catalog No. CSB-E11706r (96T) This immunoassay kit allows for the in vitro quantitative determination of rat Cholesterol concentrations in serum, plasma and other biological fluids.

More information

STUDIES OF THE HEMAGGLUTININ OF HAEMOPHILUS PERTUSSIS HIDEO FUKUMI, HISASHI SHIMAZAKI, SADAO KOBAYASHI AND TATSUJI UCHIDA

STUDIES OF THE HEMAGGLUTININ OF HAEMOPHILUS PERTUSSIS HIDEO FUKUMI, HISASHI SHIMAZAKI, SADAO KOBAYASHI AND TATSUJI UCHIDA STUDIES OF THE HEMAGGLUTININ OF HAEMOPHILUS PERTUSSIS HIDEO FUKUMI, HISASHI SHIMAZAKI, SADAO KOBAYASHI AND TATSUJI UCHIDA The National Institute of Health, Tokyo, Japan (Received: August 3rd, 1953) INTRODUCTION

More information

NM interference in the DCF assay

NM interference in the DCF assay Project: VIGO NM interference in the DCF assay Quenching effects DCF AUTHORED BY: DATE: Cordula Hirsch 20.01.14 REVIEWED BY: DATE: Harald Krug 09.04.2014 APPROVED BY: DATE: DOCUMENT HISTORY Effective Date

More information

Human Obestatin ELISA

Human Obestatin ELISA K-ASSAY Human Obestatin ELISA For the quantitative determination of obestatin in human serum and plasma Cat. No. KT-495 For Research Use Only. 1 Rev. 081309 K-ASSAY PRODUCT INFORMATION Human Obestatin

More information

An evaluation of two new haemagglutination tests for the rapid diagnosis of autoimmune thyroid diseases

An evaluation of two new haemagglutination tests for the rapid diagnosis of autoimmune thyroid diseases Journal of Clinical Pathology, 1978, 31, 1147-115 An evaluation of two new haemagglutination tests for the rapid diagnosis of autoimmune thyroid diseases I. CAYZER, S. R. CHALMERS, D. DONIACH', AND G.

More information

BIOL 347L Laboratory Three

BIOL 347L Laboratory Three Introduction BIOL 347L Laboratory Three Osmosis in potato and carrot samples Osmosis is the movement of water molecules through a selectively permeable membrane into a region of higher solute concentration,

More information

Purity Tests for Modified Starches

Purity Tests for Modified Starches Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 82 nd meeting 2016 Purity Tests for Modified Starches This monograph was also published in: Compendium

More information

CELLULASE from PENICILLIUM FUNICULOSUM

CELLULASE from PENICILLIUM FUNICULOSUM CELLULASE from PENICILLIUM FUNICULOSUM Prepared at the 55th JECFA (2000) and published in FNP 52 Add 8 (2000), superseding tentative specifications prepared at the 31st JECFA (1987) and published in FNP

More information

The following protocol describes the isolation of nuclei from tissue. Item. Catalog No Manufacturer

The following protocol describes the isolation of nuclei from tissue. Item. Catalog No Manufacturer SOP: Nuclei isolation from tissue and DNaseI treatment Date modified: 090923 Modified by: P. Sabo. (UW) The following protocol describes the isolation of nuclei from tissue. Ordering Information Item.

More information

SUPPLEMENTAL MATERIAL. Supplementary Methods

SUPPLEMENTAL MATERIAL. Supplementary Methods SUPPLEMENTAL MATERIAL Supplementary Methods Culture of cardiomyocytes, fibroblasts and cardiac microvascular endothelial cells The isolation and culturing of neonatal rat ventricular cardiomyocytes was

More information

ab Sphingomyelin Assay Kit

ab Sphingomyelin Assay Kit ab133118 - Sphingomyelin Assay Kit Instructions for Use For the specific, sensitive and convenient method of quantifying Sphingomyelin in plasma or serum. This product is for research use only and is not

More information

Biodiversity Study & Biomass Analysis

Biodiversity Study & Biomass Analysis PR072 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Biodiversity Study & Biomass Analysis Teacher s Guidebook (Cat. # BE-403) think proteins!

More information

HYPOGLYCAEMIC ACTION OF THE FLAVONOID FRACTION OF ARTOCARPUS HETEROPHYLLUS LEAF

HYPOGLYCAEMIC ACTION OF THE FLAVONOID FRACTION OF ARTOCARPUS HETEROPHYLLUS LEAF 42 Research Paper ISSN 0189-6016 2006 Afr. J. Traditional, Complementary and Alternative Medicines www.africanethnomedicines.net HYPOGLYCAEMIC ACTION OF THE FLAVONOID FRACTION OF ARTOCARPUS HETEROPHYLLUS

More information

Organic Molecule Composition of Milk: Lab Investigation

Organic Molecule Composition of Milk: Lab Investigation Name: Organic Molecule Composition of Milk: Lab Investigation Introduction & Background Milk & milk products have been a major food source from earliest recorded history. Milk is a natural, nutritionally

More information

Phytochemical screening and antibacterial properties of Garcinia kola

Phytochemical screening and antibacterial properties of Garcinia kola 2013; 2(3): 34-38 Online at: www.phytopharmajournal.com Research Article ISSN 2230-480X JPHYTO 2013; 2(3): 34-38 2013, All rights reserved Phytochemical screening and antibacterial properties of Garcinia

More information

Formulation and evaluation of sublingual tablets of lisinopril

Formulation and evaluation of sublingual tablets of lisinopril Journal of GROVER Scientific & Industrial AGARWAL: Research FORMULATION AND EVALUATION OF SUBLINGUAL TABLETS OF LISINOPRIL Vol. 71, June 2012, pp. 413-417 413 Formulation and evaluation of sublingual tablets

More information

Supplementary Files S1 Isolation of Monocytes S2 Haemolysis study Reagents Procedure S3 Cytotoxicity studies Trypan blue dye exclusion method

Supplementary Files S1 Isolation of Monocytes S2 Haemolysis study Reagents Procedure S3 Cytotoxicity studies Trypan blue dye exclusion method Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2014 Supplementary Files S1 Isolation of Monocytes A 3 ml volume of Histopaque 1083 solution was

More information

Superoxide Dismutase Assay Kit

Superoxide Dismutase Assay Kit Superoxide Dismutase Assay Kit Catalog Number KA3782 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 General Information...

More information

Mouse primary keratinocytes preparation

Mouse primary keratinocytes preparation Mouse primary keratinocytes preparation 1. Fill a 150 X 25 mm petri dish with ice. Put newborn mice (2 3 days old) in the petri dish and insert it in an ice bucket. Leave the mice in the ice bucket for

More information

African Journal of Pharmaceutical Research & Development Vol. 4 No.1 pp (2012)

African Journal of Pharmaceutical Research & Development Vol. 4 No.1 pp (2012) African Journal of Pharmaceutical Research & Development Vol. 4 No.1 pp.16-19 (2012) Effects of Antimalarial Herbal Mixture (Abc 123) on the Liver of Rats *Iliya HA, Wannang NN, Andrew MM, Ibrahim H Department

More information

International Journal of Food Nutrition and Safety, 2012, 1(2): International Journal of Food Nutrition and Safety

International Journal of Food Nutrition and Safety, 2012, 1(2): International Journal of Food Nutrition and Safety International Journal of Food Nutrition and Safety, 2012, 1(2): 54-59 International Journal of Food Nutrition and Safety Journal homepage: www.modernscientificpress.com/journals/ijfns.aspx ISSN: 2165-896X

More information

STABILITY INDICATING ASSAY. differentiate an intact drug from its potential decomposition products 425.

STABILITY INDICATING ASSAY. differentiate an intact drug from its potential decomposition products 425. .1. INTRODUCTION.1.1 STABILITY INDICATING ASSAY The stability - indicating assay is a method that is employed for the analysis of stability samples in pharmaceutical industry. It is essential to validate

More information

SUPPLEMENTARY INFORMATION. CXCR4 inhibitors could benefit to HER2 but not to Triple-Negative. breast cancer patients

SUPPLEMENTARY INFORMATION. CXCR4 inhibitors could benefit to HER2 but not to Triple-Negative. breast cancer patients SUPPLEMENTARY INFORMATION CXCR4 inhibitors could benefit to HER2 but not to Triple-Negative breast cancer patients Lefort S. 1,2, Thuleau A. 3, Kieffer Y. 1,2, Sirven P. 1,2, Bieche I. 4, Marangoni E.

More information

ASSAY OF USING BETA-GLUCAZYME TABLETS

ASSAY OF USING BETA-GLUCAZYME TABLETS ASSAY OF endo-β-glucanases USING BETA-GLUCAZYME TABLETS T-BGZ 12/12 Note: Changed assay format for malt β-glucanase Megazyme International Ireland 2012 SUBSTRATE: The substrate employed is Azurine-crosslinked

More information

Supporting Information File S2

Supporting Information File S2 Pulli et al. Measuring Myeloperoxidase Activity in Biological Samples Page 1 of 6 Supporting Information File S2 Step-by-Step Protocol Reagents: Sucrose (Sigma, S3089) CaCl 2 (Sigma, C5770) Heparin Sodium

More information

PRODUCT INFORMATION & MANUAL

PRODUCT INFORMATION & MANUAL PRODUCT INFORMATION & MANUAL Mitochondrial Extraction Kit NBP2-29448 Research use only. Not for diagnostic or therapeutic procedures www.novusbio.com P: 303.760.1950 P: 888.506.6887 F: 303.730.1966 technical@novusbio.com

More information

Bioprospecting of Neem for Antimicrobial Activity against Soil Microbes

Bioprospecting of Neem for Antimicrobial Activity against Soil Microbes ISSN: 2454-132X Impact factor: 4.295 (Volume3, Issue1) Available online at: www.ijariit.com Bioprospecting of Neem for Antimicrobial Activity against Soil Microbes R. Prasanna PRIST University, Tamilnadu

More information

Effects of glucose concentration in the medium on rat hepatocyte culture

Effects of glucose concentration in the medium on rat hepatocyte culture ORIGINAL ARTICLE pissn 2288-6575 eissn 2288-6796 http://dx.doi.org/10.4174/astr.2014.87.2.53 Annals of Surgical Treatment and Research Effects of glucose concentration in the medium on rat hepatocyte culture

More information

Blood Urea Nitrogen Enzymatic Kit Manual Catalog #:

Blood Urea Nitrogen Enzymatic Kit Manual Catalog #: Blood Urea Nitrogen Enzymatic Kit Manual Catalog #: 5602-01 TABLE OF CONTENTS GENERAL INFORMATION... 2 Product Description... 2 Procedure Overview... 2 Kit Contents, Storage and Shelf Life... 3 Required

More information

Study of the main chemical components of Ganoderma lucidum

Study of the main chemical components of Ganoderma lucidum Study of the main chemical components of Ganoderma lucidum Yasuo Komota et al Tokyo Medical and Dental University [Purpose] As part of the means for exerting quality control on Ganoderma lucidum 50% ethanol

More information

Work-flow: protein sample preparation Precipitation methods Removal of interfering substances Specific examples:

Work-flow: protein sample preparation Precipitation methods Removal of interfering substances Specific examples: Dr. Sanjeeva Srivastava IIT Bombay Work-flow: protein sample preparation Precipitation methods Removal of interfering substances Specific examples: Sample preparation for serum proteome analysis Sample

More information

The Aloe Vera Specialist

The Aloe Vera Specialist The Aloe Vera Specialist Ready-To-Mix Formula Direct from Manufacturer With Ingredients Included: Aloe Solids, Flavoring, Sweeteners, Gums, ph Buffers, etc. www.aloequeen.com 1 Rise in Aloe Vera Consumption

More information

GB Translated English of Chinese Standard: GB NATIONAL STANDARD

GB Translated English of Chinese Standard: GB NATIONAL STANDARD Translated English of Chinese Standard: GB5009.5-2016 www.chinesestandard.net Sales@ChineseStandard.net GB NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA GB 5009.5-2016 National food safety standard

More information

Experiment 1. Isolation of Glycogen from rat Liver

Experiment 1. Isolation of Glycogen from rat Liver Experiment 1 Isolation of Glycogen from rat Liver Figure 35: FIG-2, Liver, PAS, 100x. Note the presence of a few scattered glycogen granules (GG). Objective To illustrate the method for isolating glycogen.

More information

B. 50 mm Calcium Chloride Solution (CaCl 2 ) (Prepare 25 ml in Reagent A using Calcium Chloride, Dihydrate, Sigma Prod. No. C-3881.

B. 50 mm Calcium Chloride Solution (CaCl 2 ) (Prepare 25 ml in Reagent A using Calcium Chloride, Dihydrate, Sigma Prod. No. C-3881. SIGMA QUALITY CONTROL TEST PROCEDURE ProductInformation Enzymatic Assay of PHOSPHOLIPASE C PRINCIPLE: L-α-Lecithin + H 2 O Phospholipase C > 1,2-Diglyceride + Choline Phosphate Choline phosphate + H 2

More information

Choline Assay Kit (Fluorometric)

Choline Assay Kit (Fluorometric) Product Manual Choline Assay Kit (Fluorometric) Catalog Number MET- 5042 96 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Choline is a water soluble amine that is an essential

More information

Study of the main chemical components of Ganoderma lucidum

Study of the main chemical components of Ganoderma lucidum Study of the main chemical components of Ganoderma lucidum Yasuo Komota et al Tokyo Medical and Dental University [Purpose] As part of the means for exerting quality control on Ganoderma lucidum 50% ethanol

More information

--> Buy True-PDF --> Auto-delivered in 0~10 minutes. GB Translated English of Chinese Standard: GB5009.

--> Buy True-PDF --> Auto-delivered in 0~10 minutes. GB Translated English of Chinese Standard: GB5009. Translated English of Chinese Standard: GB5009.259-2016 www.chinesestandard.net Sales@ChineseStandard.net NATIONAL STANDARD GB OF THE PEOPLE S REPUBLIC OF CHINA National food safety standard Determination

More information

CoQ10(Coenzyme Q10) ELISA Kit

CoQ10(Coenzyme Q10) ELISA Kit CoQ10(Coenzyme Q10) ELISA Kit Catalogue No.: EU0196 Size: 48T/96T Reactivity: Universal Detection Range: 0.781-50ng/ml Sensitivity:

More information

National Standard of the People s Republic of China. National food safety standard. Determination of pantothenic acid in foods for infants and

National Standard of the People s Republic of China. National food safety standard. Determination of pantothenic acid in foods for infants and National Standard of the People s Republic of China GB 5413.17 2010 National food safety standard Determination of pantothenic acid in foods for infants and young children, milk and milk products Issued

More information

The Next Generation of Time Lapse Systems. Miri TL. time lapse incubator for IVF. Designed in Denmark. Made in the E.U.

The Next Generation of Time Lapse Systems. Miri TL. time lapse incubator for IVF. Designed in Denmark. Made in the E.U. The Next Generation of Time Lapse Systems. Miri TL time lapse incubator for IVF Designed in Denmark Made in the E.U. Medical Devices 1023 2 Miri TL An affordable time lapse incubation system for IVF The

More information

Mechanism of Action of N-Acetylcysteine in the Protection Against the Hepatotoxicity of Acetaminophen in Rats In Vivo

Mechanism of Action of N-Acetylcysteine in the Protection Against the Hepatotoxicity of Acetaminophen in Rats In Vivo Mechanism of Action of N-Acetylcysteine in the Protection Against the Hepatotoxicity of Acetaminophen in Rats In Vivo BERNHARD H. LAUTERBURG, GEORGE B. CORCORAN, and JERRY R. MITCHELL, Baylor College of

More information

FULVIC ACIDS. Miessence vs. Competitors. A Comparative Analysis. Introduction

FULVIC ACIDS. Miessence vs. Competitors. A Comparative Analysis. Introduction Miessence vs. Competitors A Comparative Analysis Introduction Miessence Fulvic Acids, was developed using innovative technology that separates humic substances into the fractions of our choosing. The fundamentals

More information

Pharmaceutical. Calculations

Pharmaceutical. Calculations u E L E V E N T H E D I T I O N Pharmaceutical Calculations Howard C. Ansel, Ph. D. Professor and Dean Emeritus College of Pharmacy The University of Georgia Athens, Georgia Mitchell J. Stoklosa, A.M.,

More information

10.00 PBS OVA OVA+isotype antibody 8.00 OVA+anti-HMGB1. PBS Methatroline (mg/ml)

10.00 PBS OVA OVA+isotype antibody 8.00 OVA+anti-HMGB1. PBS Methatroline (mg/ml) RESEARCH ARTICLE Penh (100% of PBS) 1 PBS 8.00 +anti-hmgb1 6.00 4.00 p=0.054 Cellular & Molecular Immunology advance online publication, PBS 3.12 6.25 Methatroline (mg/ml) Neutrophil isolation and culture

More information

Journal of Chemical and Pharmaceutical Research

Journal of Chemical and Pharmaceutical Research Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research J. Chem. Pharm. Res., 2010, 2(5): 45-51 ISSN No: 0975-7384 CODEN(USA): JCPRC5 Simultaneous U.V. Spectrophotometric estimation

More information

Short Term Programme Centre for Herbal Standardization Universiti Sains Malaysia

Short Term Programme Centre for Herbal Standardization Universiti Sains Malaysia Short Term Programme Centre for Herbal Standardization Universiti Sains Malaysia Back to the roots through your 1- stop multicultural herbal hub Centre for Herbal Standardization (CHEST) Universiti Sains

More information

-Glucan (mixed linkage), colorimetric method

-Glucan (mixed linkage), colorimetric method -Glucan (mixed linkage), colorimetric method Catalogue number: AK0027, 00 tests Introduction -Glucans are common components in cereals, bacteria, yeasts and mushrooms. Mixed linkage -glucans are naturally

More information

Asian Journal of Pharmaceutical and Clinical Research Vol. 4, Issue 2, 2011 ISSN

Asian Journal of Pharmaceutical and Clinical Research Vol. 4, Issue 2, 2011 ISSN Asian Journal of Pharmaceutical and Clinical Research Vol. 4, Issue 2, 2011 ISSN - 0974-2441 Research Article HERB DRUG INTERACTION STUDIES OF EURYCOMA LONGIFOLIA EXTRACT TAF 273 ON THE METABOLISM OF ROSIGLITAZONE,

More information

Plant tissue extraction kit. For extraction of soluble protein and other biomolecules and metabolites from plant tissues.

Plant tissue extraction kit. For extraction of soluble protein and other biomolecules and metabolites from plant tissues. ab206999 Plant tissue extraction kit Instructions for use: For extraction of soluble protein and other biomolecules and metabolites from plant tissues. This product is for research use only and is not

More information

ENHANCEMENT OF SOLUBILITY OF BICALUTAMIDE DRUG USING SOLID DISPERSION TECHNIQUE

ENHANCEMENT OF SOLUBILITY OF BICALUTAMIDE DRUG USING SOLID DISPERSION TECHNIQUE PHARMA SCIENCE MONITOR AN INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES ENHANCEMENT OF SOLUBILITY OF BICALUTAMIDE DRUG USING SOLID DISPERSION TECHNIQUE Kantilal B. Narkhede *1, R. B. Laware 2, Y. P.

More information

March, 2000 Volume 9, No. 1 RESEARCH ODDS & ENDS

March, 2000 Volume 9, No. 1 RESEARCH ODDS & ENDS Research Notes A C o m p i l a t i o n o f V i t a l R e s e a r c h U p d a t e s o n H u m a n N u t r i t i o n March, 2000 Volume 9, No. 1 RESEARCH ODDS & ENDS As many know, Albion is heavily involved

More information

Mouse Adiponectin Immunoassay Kit

Mouse Adiponectin Immunoassay Kit Antibody and Immunoassay Services Li Ka Shing Faculty of Medicine, The University of Hong Kong Mouse Adiponectin Immunoassay Kit Catalogue Number: 32010 For the quantitative determination of mouse adiponectin

More information

Journal of Chemical and Pharmaceutical Research, 2017, 9(3): Research Article

Journal of Chemical and Pharmaceutical Research, 2017, 9(3): Research Article Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2017, 9(3):214-218 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Extraction Optimization and Antioxidant Activity

More information

Optimization of Enzyme-assisted Ultrasonic Extraction of Total Ginsenosides from Ginseng Roots Guangna LIU, Yulin ZUO, Jing ZHANG

Optimization of Enzyme-assisted Ultrasonic Extraction of Total Ginsenosides from Ginseng Roots Guangna LIU, Yulin ZUO, Jing ZHANG 2019 2nd International Conference on Computer Science and Advanced Materials (CSAM 2019) Optimization of Enzyme-assisted Ultrasonic Extraction of Total Ginsenosides from Ginseng Roots Guangna LIU, Yulin

More information

The effect of insulin on chemotherapeutic drug sensitivity in human esophageal and lung cancer cells

The effect of insulin on chemotherapeutic drug sensitivity in human esophageal and lung cancer cells The effect of insulin on chemotherapeutic drug sensitivity in human esophageal and lung cancer cells Published in: Natl Med J China, February 10, 2003; Vol 83, No 3, Page 195-197. Authors: JIAO Shun-Chang,

More information

Islet viability assay and Glucose Stimulated Insulin Secretion assay RT-PCR and Western Blot

Islet viability assay and Glucose Stimulated Insulin Secretion assay RT-PCR and Western Blot Islet viability assay and Glucose Stimulated Insulin Secretion assay Islet cell viability was determined by colorimetric (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay using CellTiter

More information

IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page:

IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: ISSN: 2320-2831 Research article Open Access Method development and validation of tenofovir disoproxil fumerate and emtricitabine in combined tablet

More information

DEVELOPMENT OF NEW ANALYTICAL METHODS FOR THE ESTIMATION OF OPIOID ANTAGONIST NALTREXONE HYDROCHLORIDE

DEVELOPMENT OF NEW ANALYTICAL METHODS FOR THE ESTIMATION OF OPIOID ANTAGONIST NALTREXONE HYDROCHLORIDE 114 P a g e International Standard Serial Number (ISSN): 2319-8141 International Journal of Universal Pharmacy and Bio Sciences 4(1): January-February 2015 INTERNATIONAL JOURNAL OF UNIVERSAL PHARMACY AND

More information

Supplemental Information

Supplemental Information Supplemental Information SI1. AFM Imaging and SPR Imaging Before and After Exposure to Enzyme Mixture in the Sample Chamber and Buffer in the Reference Chamber of the Fluid Cell To verify that cellulose

More information

EPIGENTEK. EpiQuik Total Histone Extraction Kit. Base Catalog # OP-0006 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE

EPIGENTEK. EpiQuik Total Histone Extraction Kit. Base Catalog # OP-0006 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE EpiQuik Total Histone Extraction Kit Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE Uses: The EpiQuik Total Histone Extraction Kit is suitable for a quick preparation of total histone extracts

More information

European Food Safety Authority

European Food Safety Authority AF 01.10.2004 3a Discussion paper on Botanicals European Food Safety Authority SCIENTIFIC COMMITTEE Brussels, 25 June 2004 EFSA/SC/26 Final Discussion Paper on Botanicals and Botanical Preparations widely

More information

Human Alpha 1 microglobulin ELISA Kit

Human Alpha 1 microglobulin ELISA Kit Human Alpha 1 microglobulin ELISA Kit Catalogue No.: EH4144 Size: 48T/96T Reactivity: Human Range:0.625-40ng/ml Sensitivity:

More information

Acetoacetate Assay Kit (Colorimetric)

Acetoacetate Assay Kit (Colorimetric) ab180875 Acetoacetate Assay Kit (Colorimetric) Instructions for Use For the rapid, sensitive and accurate measurement of endogenous Acetoacetate in various samples. This product is for research use only

More information

EFFECTS OF VANADATE ON OLEIC ACID INDUCED INSULIN RESISTANCE IN CULTURED RAT HEPATOCYTES

EFFECTS OF VANADATE ON OLEIC ACID INDUCED INSULIN RESISTANCE IN CULTURED RAT HEPATOCYTES 1 Department of Physiology, School of Dentistry, University of Zagreb, Gunduliæeva 5, HR-1 Zagreb, Croatia Department of Physiology, School of Medicine, University of Zagreb, Šalata 3, HR-1 Zagreb, Croatia

More information

Synopsis. Received March 2, adrenaline. Mosinger and Kujalova (1964) reported that adrenaline-induced lipolysis

Synopsis. Received March 2, adrenaline. Mosinger and Kujalova (1964) reported that adrenaline-induced lipolysis Studies on Reduction of Lipolysis in Adipose Tissue on Freezing and Thawing YASUSHI SAITO1, NoBUO MATSUOKA1, AKIRA KUMAGAI1, HIROMICHI OKUDA2, AND SETSURO FUJII3 Chiba University, Chiba 280, Japan, 2Department

More information

NOVEL RP-HPLC METHOD. B.Lakshmi et a. concentration range KEY INTRODUCTION. Diltiazem is used to. . It works by of contractionn of the. (dilate).

NOVEL RP-HPLC METHOD. B.Lakshmi et a. concentration range KEY INTRODUCTION. Diltiazem is used to. . It works by of contractionn of the. (dilate). B.Lakshmi et a ISSN-2319-2119 al, The Experiment, January. 2013 Vol..6(4), 365-371 A NOVEL RP-HPLC METHOD FOR THE DETERMINATION OF DILTIAZEM IN PHARMACEUTICAL DRUG PRODUCTS ABSTRACT High resolution RP-HPLC

More information

A COMPARATIVE STUDY OF XENOBIOTIC-METABOLIZING ENZYMES IN LIVER AND INTESTINE OF VARIOUS ANIMAL SPECIES

A COMPARATIVE STUDY OF XENOBIOTIC-METABOLIZING ENZYMES IN LIVER AND INTESTINE OF VARIOUS ANIMAL SPECIES DRUG METABOLISM A DIsPOsITIoN Copyright #{174} 1974 by The American Society for Pharmacology and Experimental Therapeutics Vol. 2 No. S Printed in U.S.A. A COMPARATIVE STUDY OF XENOBIOTIC-METABOLIZING

More information

Activity # 4. Measurement of Enzyme Activity

Activity # 4. Measurement of Enzyme Activity Activity # 4. Measurement of Enzyme Activity Adapted from Experimental Molecular Biology Lab Manual, Stephen H. Munroe, Marquette University Week 1 Protein Extraction, Concentration Measurement & Instrument

More information

AF HDL and LDL/VLDL Assay Kit

AF HDL and LDL/VLDL Assay Kit A HDL and LDL/VLDL Assay Kit Catalog Number KA1656 100 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3

More information

1.2 Systematic Name: Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

1.2 Systematic Name: Orthophosphoric-monoester phosphohydrolase (alkaline optimum) Document Title Alkaline Phosphatase Page 1 of 6 Originating Department QA Approval Departments QA, QC Approval Date 5 th June 2017 Effective Date 8 th June 2017 1.0 PRODUCT DETAILS 1.1 Enzyme Name: Alkaline

More information

NISIN. SYNONYMS INS No. 234 DEFINITION

NISIN. SYNONYMS INS No. 234 DEFINITION SYNONYMS INS No. 234 NISIN Prepared at the 12th JECFA (1968), published in NMRS 45A (1969) and in FNP 52 (1992). Metals and arsenic specifications revised at the 63rd JECFA (2004). An ADI 0-33,000 units

More information

Studies on the metabolism of volatile fatty acids in hens

Studies on the metabolism of volatile fatty acids in hens Studies on the metabolism of volatile fatty acids in hens II. An effect of feedstuff on the production of V.F. As in the alimentary tract of egg laying hens Eiichi WATANABE and Akira CHIBA (Miyagi Agriculture

More information

THE QUANTITATIVE GLUCOSE AND MINERAL NUTRIENT REQUIREMENTS OF MOUSE LS (SUSPENSION) CELLS IN CHEMICALLY DEFINED MEDIUM

THE QUANTITATIVE GLUCOSE AND MINERAL NUTRIENT REQUIREMENTS OF MOUSE LS (SUSPENSION) CELLS IN CHEMICALLY DEFINED MEDIUM J. Cell Sci. 8, 693-700 (1971) Printed in Great Britain THE QUANTITATIVE GLUCOSE AND MINERAL NUTRIENT REQUIREMENTS OF MOUSE LS (SUSPENSION) CELLS IN CHEMICALLY DEFINED MEDIUM J. R. BIRCH* AND S. J. PIRT

More information

HYPOLIPIDEMIC EFFECT OF EXTRACTS FROM ABELMOSCHUS ESCULENTUS L. MALVACEAE ON TYLOXAPOL- INDUCED HYPERLIPIDEMIA IN MICE

HYPOLIPIDEMIC EFFECT OF EXTRACTS FROM ABELMOSCHUS ESCULENTUS L. MALVACEAE ON TYLOXAPOL- INDUCED HYPERLIPIDEMIA IN MICE HYPOLIPIDEMIC EFFECT OF EXTRACTS FROM ABELMOSCHUS ESCULENTUS L. MALVACEAE ON TYLOXAPOL- INDUCED HYPERLIPIDEMIA IN MICE Huynh Ngoc Trinh 1, Nguyen Ngoc Quynh 1, Tran T Van Anh 2, Vo Phung Nguyen 1 1 Department

More information

SensoLyte pnpp Alkaline Phosphatase Assay Kit *Colorimetric*

SensoLyte pnpp Alkaline Phosphatase Assay Kit *Colorimetric* SensoLyte pnpp Alkaline Phosphatase Assay Kit *Colorimetric* Catalog # 72146 Kit Size 500 Assays (96-well plate) Optimized Performance: This kit is optimized to detect alkaline phosphatase activity Enhanced

More information

ASSAY OF using AZO-FRUCTAN S-AZFR5 11/17

ASSAY OF using AZO-FRUCTAN S-AZFR5 11/17 www.megazyme.com ASSAY OF endo-fructanase using AZO-FRUCTAN S-AZFR5 11/17 Megazyme 2017 PRINCIPLE: The substrate is the high molecular weight fraction of chicory fructan (DP ~ 20-60) dyed with an azo-dye

More information