Pelagia Research Library
|
|
- Nora Stevenson
- 6 years ago
- Views:
Transcription
1 Available online at Der Pharmacia Sinica, 2012, 3 (1):41-46 ISSN: CDEN (USA): PSHIBD Determination of withanolides from the roots and herbal formulation of Withania somnifera by HPLC using DAD and ELSD detector J. V. Manwar 1, K. R. Mahadik 1*, A. R. Paradkar 2, S. P. Takle 3, L. Sathiyanarayanan 1 and S. V. Patil 3 1 Department of Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Pune, Maharashtra, India 2 Institute of Pharmaceutical Innovation, and IRC in Polymer Science and Technology, University of Bradford, Bradford, West Yorkshire- BD7 1DP, United Kingdom 3 Department of Alcohol Technology, Vasantdada Sugar Institute, Pune, Maharashtra, India ABSTRACT Roots of Withania somnifera are widely used in Indian herbal formulations. Withaferine A and withanolide A are two pharmacologically important withanolides present in the roots of the plant. HPLC method using DAD and ELSD detector was developed for simultaneous determination of withaferine A and withanolide A from the roots and herbal formulation of the plant. Coarse powder of root was extracted with methanol by shaking for 30 minutes and sonication for 45 minutes. Herbal formulation was first extracted with chloroform and then with ethyl acetate, extracts were combined, evaporated and reconstituted with methanol. Retention time of both the compounds was vary with detector to detector and it was found for withaferine A (3.74, 3.84 min) and withanolide A (4.51, 4.60 min) with DAD and ELSD detector, respectively. Amount of withanolides in each sample were calculated from the regression equations of standard calibration graphs. The results showed that the HPLC method is suitable for the determination of withaferine A and withanolide A from the roots and herbal formulation of W. somnifera. Keywords: Withaferine A, withanolide A, Withania somnifera, HPLC. INTRDUCTIN Withania somnifera (family Solanaceae) is a medicinally important herb used in number of Indian herbal formulations. In India, it is locally known as Ashwagandha and is considered as Indian Ginseng. Roots of the plant are major source of active chemical substances and are traditionally used to cure ulcers, fever, cough, dyspnoea, consumption, dropsy, impotence, rheumatism, toxicosis and leucoderma [1]. These activities are mainly attributed towards the presence of different withanolides mainly withaferine A and withanolide A [2-5]. Ashwagandharishtha is the herbal formulation that contains roots of W. somnifera as a major crude drug along with 26 other herbs. It is mainly recommended for the treatment of impotency. ther uses of the formulation include syncope, epilepsy, cachexia, psychosis, emaciation, piles, digestive impairment, and neurological disorders [6-7]. Quality of the crude drug used in manufacturing of such formulations depends on the amount of active ingredient presents. Literature survey indicated phytochemical variability in marketed preparations of W. somnifera [8]. Therefore, standardization 41
2 of these crude drugs has become necessary before their use for preparing formulations. Moreover, standardization of finished product is of equal importance to be considered in assuming the safety and efficacy product before use. Evaporative Light Scattering detector (ELSD) is a sensitive and a universal detector which eliminates the need of derivatization for low or non UV absorbing compounds [9]. Literature survey shows that the HPLC and HPTLC method is available for the determination of withaferine A from W. somnifera [10-12]. For simultaneous determination of withaferine A with withanolide D, RP-HPLC method is reported [13]. HPLC-UV (DAD)-positive ion electrospray ionization-mass spectrometry is available for determination of withaferine A, 12- deoxywithastramolides, withanolide A using withanone as external standard [14]. This method is costly and time consuming as separation time is 35 minutes. ne TLC method is reported for simultaneous determination withaferine A and withanolide A from different plant parts of W. somnifera. This method is time consuming, non reproducible and requires derivatization of compounds prior to analysis [15]. Both the withanolides are low UV absorbing compounds can be accurately analyzed by ELSD detector even at very low concentration. HPLC is the most commonly used technique for the separation and analysis of pharmaceutical and phytochemical compounds [16-18]. It possesses advantages like high resolving power, speed of separation, accuracy and reproducibility. In the literature, there is no report of HPLC method for the simultaneous determination of withaferine A and withanolide A in roots and herbal formulation of W. somnifera. Therefore, in the present paper we have reported simple, rapid and reproducible HPLC method for the determination of these withanolides from the roots and herbal formulation of W. somnifera. (I) CH 2 H (II) H H H H H Structure of withaferine A (I) and withanolide A (II) H MATERIALS AND METHDS Plant materials and reagents Dried roots of W. somnifera were collected from local market and were authenticated by the Agharkar Research Institute, Pune, India. The roots were first crushed into small pieces and then converted to coarse powder by pulverization. Herbal formulation Ashwagandharishtha (Baidyanath) was procured from local Ayurvedic Pharmacy. Standard drug sample of withaferine A and withanolide A were purchased from Natural Remedies Private Limited, Bangalore, India. All the solvents used in the study were of HPLC grade. Preparation of standard solution Standard withaferine A and withanolide A were dissolved methanol to produce concentration of each 1000 µg/ml (standard stock solution). 1 ml of the resultant solution was further diluted to 10 ml to get a final concentration containing 100 µg/ml of each drug. This concentration was used for the experimental work (standard solution). Extraction of samples The coarse powder of W. somnifera (1g) was extracted with 10 ml of methanol by shaking for 30 minutes and then sonicated (Bandelin Sonorex sonicator) for 45 minutes at room temperature. The extract was concentrated to dryness by removing the solvents using rotary vacuum evaporator (Laborota 4000, Heidolph). The extract was redissolved in 10 ml of methanol, filtered through 0.45 micron filter paper and was used for analysis. For herbal formulation, 10 ml of product was successively extracted thrice with each of 10 ml chloroform and then with each of 10 ml of ethyl acetate. Both the extracts were combined, concentrated to dryness by removing the solvents using rotary vacuum evaporator and redissolved in 10 ml of methanol. The solution was filtered through 0.45 micron filter paper and was used for analysis. 42
3 TLC analysis of withanolides Both the extracts were analyzed by thin layer chromatography using CAMAG HPTLC system comprising of Linomat-IV sample applicator, scanner III with WinCATS software for scanning and documentation. About 5 µl of standard solution, 50 µl of both extracts were spotted separately on TLC plate precoated with silica gel GF 254 (5 x 10 cm). The development of plate and measurement of peak areas were carried out according to procedure given by Sharma [15]. Figure 1. Densitograms of withaferine A (1) and withanolide A (2) in standard solution (A), roots extract (B) and marketed formulation (C). Densitogram of marketed formulation (C) showing masking of withanolides peaks by unknown peak (3) HPLC analysis of withanolides HPLC analysis of withaferine A and withanolide A was carried out on Agilent 1260 Infinity Quaternary LC System consist of diode array detector G4212A (DAD) and 1260 Evaporative Light Scattering detector (ELSD) with auto injector using Nucleosil C 18 (4.6 mm I.D x 250 mm) column. The mobile phase was consisting of acetonitrile and water at the ratio of 60:40 at 35 C, with a flow rate of 1.0 ml/min. The measurements of withanolides with DAD detector were made at 215 nm (Bw 4 nm) and ELSD detector at evaporation temperature 45 C and nitrogen pressure 4 bar. About 4 µl of standard solution and both sample solutions were injected separately and chromatograms were recorded. The amount of withaferine A and withanolide A present in the sample were calculated from respective regression equations. The chromatograms of standard solution, roots and herbal formulation extract are shown in Figure 2 and Figure 3. Validation of proposed method Validation of proposed method was carried out in terms of linearity and range, limit of detection (LD), limit of quantitation (LQ), precision and recovery. Calibration graph, LD and LQ To study the linearity of withanolides, a series of dilutions (60, 80, 100, 120 and 140 µg/ml) were made from the standard stock solution. Calibration graph was plotted as concentration versus peak area response. The limit of detection and limit of quantitation were measured on the basis of signal-to-noise ratios. Precision The precision of method was checked by injecting three consecutive replicates of standard solution. Precision of the method was expressed in terms of %RSD calculated from the measurement of peak area response. 43
4 Recovery study To study the accuracy of method, recovery study was carried out by standard addition method. Accuracy was evaluated in triplicate by adding known concentration of standard solution to the preanalysed sample of roots and herbal formulation. Figure 2. Chromatograms of withaferine A (1) and withanolide A (2) in standard solution by DAD (A) and ELSD detector (B) I II Figure 3. Chromatograms of withaferine A (1) and withanolide A (2) in roots (I) and herbal formulation (II) of W. somnifera by DAD (A) and ELSD detector (B) RESULTS AND DISCUSSIN Thin layer chromatography method reported by Sharma et al. [15] for quantification of withaferine A and withanolide A from roots and other parts of W. somnifera require derivatization of spots using vanillin reagent prior to detection. Peak response is depends upon the various factors like concentration and immersion time of plate in vanillin reagent, drying temperature and waiting time before detection. This whole process affects the sensitivity and reproducibility of method. Most important drawback of this method is it can only use for analysis of withanolides in various parts of plant including roots, but it cannot be used for the analysis of herbal formulation. When extracts of roots and herbal formulation were analysed by this method, it was observed that there was no interference of peaks of withanolides in roots sample, but in case of herbal formulation peaks of both withanolides get masked by big peak of unknown compound (Figure 1). Therefore, attempts were made to develop a simple, rapid and reproducible 44
5 HPLC method for determination of withaferine A and withanolide A from the roots and herbal formulation of W. somnifera using DAD and ELDS detector. Use of ELSD detector eliminates the problem of sensitivity of withanolides which are low UV absorbing. Comparison of peak areas shown by DAD and ELSD detector indicated that withaferine A is near about 1300 time more sensitive to ELSD detector than DAD detector, whereas withanolide A is more than 1900 times more sensitive to ELSD detector than DAD detector. Calibration graphs for withaferine A and withanolide A were obtained by plotting concentration versus peak area response. The graphs were found to be linear over the concentration range 60 to140 µg/ml with high correlation coefficient values ranges from to By DAD detector, observed LD and LQ values were withaferine A (2.29, 6.95 µg/ml) and withanolide A (2.07, 6.27µg/mL), respectively. By ELSD detector, LD and LQ values were withaferine A (6.95, µg/ml) and withanolide A (6.27, µg/ml). Higher LD and LQ values by ELSD detector may be due to the noisy base line. Precision of the method was expressed as %RSD and it was calculated from the peak area response by injecting three consecutive replicates of standard solution. The %RSD found out by DAD and ELSD detector were withaferine A (0.46, 0.93) and withanolide A (0.23, 0.97), respectively. The values lie within a standard limit. The data of validation parameters is given in Table 1. The contents of withanolides in roots extract and herbal formulation were calculated from the regression equations of standard calibration graphs. The %RSD of sample analysis of roots extract and herbal formulation was range from within 0.44 to The percent recovery obtained for withanolides in roots extract and herbal formulation was within the limit of to (Table 2). The results confirm the method is accurate and free from any positive or negative interference of other compounds present. The work demonstrated that proposed HPLC method is suitable for determination of withaferine A and withaferine A and withanolide A from roots and herbal formulation of W. somnifera. Table 1. Validation parameters of HPLC method for withanolides DAD detector ELSD detector Withaferine A Withanolide A Withaferine A Withanolide A Retention time Area Range (µg/ml) Linearity (R 2 ) LD (µg/ml) LQ (µg/ml) Precision Table 2. Results of analysis of withanolides in roots and herbal formulation and recovery study DAD detector ELSD detector Contents %Recovery Contents %Recovery Mean %RSD Mean %RSD Mean %RSD Mean %RSD Roots extract Withaferine A Withanolide A Herbal formulation Withaferine A Withanolide A *contents in µg/ml; n=3 REFERENCES [1] U Devi. Ind. J Pharm Sci, 1996, 34, [2] SK Bhattacharya, KS Satyan, S Ghosal, Ind J Exp Biol, 1997, 35, [3] B Jayaprakasam, YJ Zhang, NP Seeram, MG Nair, Life Sci, 2003, 21,
6 [4] H Ichikawa, Y Takada, S Shishodia, B Jayaprakasam, MG Nair, BB Agarwal, Mol Cancer Ther, 2006, 5, [5] L Misra, P Mishra, A Pandey, RS Sangwan, NS Sangwan, R Tuli, Phytochemistry, 2008, 69, [6] Anonymous, Ayurvedic Pharmacopoeia of India (1 st Edn.). The Controller of Publications, New Delhi, 2001, [7] CP Khare, Indian Medicinal Plants. Springer Science and Business Media, New York, USA, [8] RS Sangwan, ND Chaurasiya, LN Misra, P Lal, GC Uniyal, R Sharma, NS Sangwan, KA Suri, GN Qazi, R Tuli R, Current Sci, 2004, 86, [9] XY Chai, SL Li, P Li, J Chromatogr A, 2005, 1070, [10] N Mahadevan, RP Kasar, T Subburaju, B Suresh, J Sep Sci, 2003, 26, [11] S Bala, R Govindrajan, A Rawat, S Mahrotra, Ind J Pharm Sci, 2004, 66, [12] PS Nayak, S Upadhyay, A Upadhayay, J Plan Chromatogr, 2009, 22, [13] M Ganzera, MI Choudhari, IA Khan, Fitoterapia, 2003, 74, [14] R Khajuria, K Suri, R Gupta, N Satil, M Amina, Suri, G Qazi, J Sep Sci, 2004, 27, [15] V Sharma, AP Gupta, P Bhandari, RC Gupta, B Singh, Chromatogr, 2007, 66, [16] JV Manwar, BV Sonawane, SV Patil, SP Takle, Der Chemica Sinica, 2011, 2, [17] M Sugumaran, M Poornima, Y Yogesh Kumar, S Ramarajasekhar, Der Pharmacia Sinica, 2011, 2, [18] RP Mahor, V Parcha, Y Singh, R Sharma, A Bhandari, Der Chemica Sinica, 2011, 2,
Development and Validation of Stability Indicating HPTLC Method for Estimation of Seratrodast
ARC Journal of Pharmaceutical Sciences (AJPS) Volume 2, Issue 3, 2016, PP 15-20 ISSN 2455-1538 DOI: http://dx.doi.org/10.20431/2455-1538.0203004 www.arcjournals.org Development and Validation of Stability
More informationInternational Journal of Pharma and Bio Sciences DISTRIBUTIO OF WITHA OLIDE A CO TE T I VARIOUS ORGA S OF WITHA IA SOM IFERA (L.) DU AL.
International Journal of Pharma and Bio Sciences DISTRIBUTIO OF WITHA OLIDE A CO TE T I VARIOUS ORGA S OF WITHA IA SOM IFERA (L.) DU AL. N. PRAVEEN, P. M. NAIK, S. H. MANOHAR AND H. N. MURTHY* Plant laboratory,
More informationA HPTLC method for chemotaxonomic evaluation of some Curcuma Species and their commercial samples
Journal of Scientific & Industrial Research 876 Vol. 68, October 2009, pp. 876-880 J SCI IND RES VOL 68 OCTOBER 2009 A HPTLC method for chemotaxonomic evaluation of some Curcuma Species and their commercial
More informationJPSBR: Volume 4, Issue 6: 2014 ( ) ISSN NO
Analytical Method Development and Validation for Simultaneous Estimation of Timolol Maleate and Brimonidine in Bulk and Marketed Ophthalmic Formulation Suketa K. Mehta*, Dr. Dilip G. Maheshwari Department
More informationJournal of Chemical and Pharmaceutical Research, 2013, 5(1): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2013, 5(1):180-184 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 A simple and sensitive RP-HPLC method for estimation
More informationDevelopment of a Validated RP-HPLC Method for the Analysis of Citicoline Sodium in Pharmaceutical Dosage Form using Internal Standard Method
Research Article Development of a Validated RP-HPLC Method for the Analysis of Citicoline Sodium in Pharmaceutical Dosage Form using Internal Standard Method * S. M. Sandhya, G. Jyothisree, G. Babu Department
More informationDevelopment and Validation of Improved RP-HPLC method for Identification and Estimation of Ellagic and Gallic acid in Triphala churna
International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol.2, No.3, pp 1486-1493, July-Sept 2010 Development and Validation of Improved RP-HPLC method for Identification and Estimation
More informationComparative HPTLC Analysis of Leaves of Allium cepa L., Ficus carica L. and Ziziphus mauritiana L. with Standard Quercetin
Available online on www.ijppr.com International Journal of Pharmacognosy and Phytochemical Research 2017; 9(5); 739-743 DOI number: 10.25258/phyto.v9i2.8157 ISSN: 0975-4873 Research Article Comparative
More informationVol-3, Issue-4, Suppl-1, Nov 2012 ISSN: Ghodasara et al PHARMA SCIENCE MONITOR
PHARMA SCIENCE MONITOR AN INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES HPTLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF DIOSGENIN AND GALLIC ACID IN MARKETED FORMULATION Ghodasara
More informationMETHOD DEVELOPMENT AND VALIDATION BY RP-HPLC FOR ESTIMATION OF ZOLPIDEM TARTARATE
WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES Ramalakshmi et al. SJIF Impact Factor 6.647 Volume 7, Issue 2, 1010-1018 Research Article ISSN 2278 4357 METHOD DEVELOPMENT AND VALIDATION BY RP-HPLC
More informationMethod Development and Validation for the Estimation of Saroglitazar in Bulk and Pharmaceutical Dosage Form by RP-HPLC
Original Research Method Development and Validation for the Estimation of Saroglitazar in Bulk and Pharmaceutical Dosage Form by RP-HPLC Hanumantha Rao K 1, Lakshmana Rao A 2,*, Chandra Sekhar KB 3 1 Assistant
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2015, 6(1):6-10 ISSN: 0976-8688 CODEN (USA): PSHIBD Validated RP-HPLC method for simultaneous estimation of metformin hydrochloride
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2014, 5(5):91-98 ISSN: 0976-8688 CODEN (USA): PSHIBD A novel RP-HPLC method development and validation of Perindopril Erbumine in
More informationAvailable online at Universal Research Publications. All rights reserved. Original Article
Available online at http://www.urpjournals.com International Journal of Analytical and Bioanalytical chemistry Universal Research Publications. All rights reserved Original Article Application of HPTLC
More informationJournal of Chemical and Pharmaceutical Research, 2018, 10(3): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2018, 10(3):142-147 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development of Reverse Phase HPLC Method and Validation
More informationSimultaneous Estimation and Validation of Tramadol and Paracetamol by HPTLC in Pure and Pharmaceutical Dosage Form
Asian Journal of Chemistry Vol. 22, No. 2 (2010), 850-854 Simultaneous Estimation and Validation of Tramadol and Paracetamol by HPTLC in Pure and Pharmaceutical Dosage Form C. ROOSEWELT*, N. HARIHRISHNAN,
More informationScholars Research Library. Der Pharmacia Lettre, 2016, 8 (6): (http://scholarsresearchlibrary.com/archive.html)
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (6):217-223 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More information------------------------------------------------------------------------------------------------------------------------ HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC METHOD FOR QUANTIFICATION OF PIPERINE
More informationHPTLC Determination of Atomoxetine Hydrochloride from its Bulk Drug and Pharmaceutical Preparations
Asian Journal of Chemistry Vol. 20, No. 7 (2008), 5409-5413 HPTLC Determination of Atomoxetine Hydrochloride from its Bulk Drug and Pharmaceutical Preparations S.S. KAMAT, VINAYAK T. VELE, VISHAL C. CHOUDHARI
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS TOLBUTAMIDE IN PURE AND PHARMACEUTICAL FORMULATIONS
Int. J. Chem. Sci.: 11(4), 2013, 1607-1614 ISSN 0972-768X www.sadgurupublications.com DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS TOLBUTAMIDE IN PURE AND PHARMACEUTICAL FORMULATIONS
More informationRP-HPLC Analysis of Temozolomide in Pharmaceutical Dosage Forms
Asian Journal of Chemistry Vol. 22, No. 7 (2010), 5067-5071 RP-HPLC Analysis of Temozolomide in Pharmaceutical Dosage Forms A. LAKSHMANA RAO*, G. TARAKA RAMESH and J.V.L.N.S. RAO Department of Pharmaceutical
More informationTHIN LAYER CHROMATOGRAPHY
THIN LAYER CHROMATOGRAPHY Thin layer chromatography is the best known technique of plant biochemistry. TLC is used for preliminary separation and determination of plant constituents. It is helpful for
More informationDevelopment and Validation of HPTLC Method for Estimation of Tramadol HCl in Bulk and in Capsule Dosage Form
International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.4, No.3, pp 1261-1265, July-Sept 2012 Development and Validation of HPTLC Method for Estimation of Tramadol HCl in Bulk
More informationSimultaneous Estimation of Gemcitabine Hydrochloride and Capecitabine Hydrochloride in Combined Tablet Dosage Form by RP-HPLC Method
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2011, 8(3), 1212-1217 Simultaneous Estimation of Gemcitabine Hydrochloride and Capecitabine Hydrochloride in Combined Tablet Dosage
More informationAsian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:
Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: www.ajpamc.com VALIDATED RP-HPLC METHOD FOR DETERMINATION OF BROMHEXINE
More informationIJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page:
IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: ISSN: 2320-2831 Research article Open Access Method development and validation of tenofovir disoproxil fumerate and emtricitabine in combined tablet
More informationISSN (Print)
Scholars Academic Journal of Pharmacy (SAJP) Sch. Acad. J. Pharm., 2014; 3(3): 240-245 Scholars Academic and Scientific Publisher (An International Publisher for Academic and Scientific Resources) www.saspublisher.com
More informationDEVELOPMENT AND VALIDATION OF NEW HPLC METHOD FOR THE ESTIMATION OF PALIPERIDONE IN PHARMACEUTICAL DOSAGE FORMS
ISSN: 0974-1496 e-issn: 0976-0083 CODEN: RJCABP http://www.rasayanjournal.com http://www.rasayanjournal.co.in DEVELOPMENT AND VALIDATION OF NEW HPLC METHOD FOR THE ESTIMATION OF PALIPERIDONE IN PHARMACEUTICAL
More informationDevelopment and Validation of Analytical Method for Determination of Andrographolide in Bulk Powder
DOI:10.21276/ijprhs.2019.01.08 Y Pancham et al. CODEN (USA)-IJPRUR, e-issn: 2348-6465 International Journal of Pharma Research and Health Sciences Available online at www.pharmahealthsciences.net Original
More informationISSN: ; CODEN ECJHAO E-Journal of Chemistry 2011, 8(3),
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2011, 8(3), 1275-1279 Simultaneous Determination of Paracetamol, Phenylephrine Hydrochloride, Oxolamine Citrate and Chlorpheniramine
More informationDevelopment and validation of stability indicating RP-LC method for estimation of calcium dobesilate in pharmaceutical formulations
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (11):236-242 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(1):138-144 Simultaneous RP HPLC determination of Latanoprost
More informationApplication Note. Agilent Application Solution Analysis of ascorbic acid, citric acid and benzoic acid in orange juice. Author. Abstract.
Agilent Application Solution Analysis of ascorbic acid, citric acid and benzoic acid in orange juice Application Note Author Food Syed Salman Lateef Agilent Technologies, Inc. Bangalore, India 8 6 4 2
More informationScholars Research Library
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2010, 2(2): 294-299 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationSIMULTANEOUS ESTIMATION OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN TABLETS BY RP-HPLC METHOD
170 Original Article SIMULTANEOUS ESTIMATION OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN TABLETS BY RP-HPLC METHOD *Lakshmana Rao A, 1 Bhaskara Raju V *V.V. Institute of Pharmaceutical Sciences, Gudlavalleru,
More informationAvailable Online through Research Article
IJPT ISSN: 0975-766X Available Online through Research Article www.ijptonline.com DETERMINATION OF GALLIC ACID IN ACACIA NILOTICA LINN. BY HPTLC V Leela *, L Kokila, R Lavanya, A.Saraswathy, P.Brindha
More informationAvailable online at Scholars Research Library
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2015, 7 (3):157-161 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationTentu Nageswara Rao et al. / Int. Res J Pharm. App Sci., 2012; 2(4): 35-40
International Research Journal of Pharmaceutical and Applied Sciences Available online at www.irjpas.com Int. Res J Pharm. App Sci., 2012; 2(4):35-40 Research Article Estimation of Fesoterodine fumarate
More informationDevelopment And Validation Of HPTLC Method For Simultaneous Estimation Of Rutin And Quercetin In Hydroalcoholic Extract Of Triphala Churna
International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.4, No.4, pp 1457-1463, Oct-Dec 2012 Development And Validation Of HPTLC Method For Simultaneous Estimation Of Rutin
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD ESTIMATION OF TOLVAPTAN IN BULK PHARMACEUTICAL FORMULATION
http://www.rasayanjournal.com Vol.4, No.1 (2011), 165-171 ISSN: 0974-1496 CODEN: RJCABP DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR AND ITS PHARMACEUTICAL FORMULATION V. Kalyana Chakravarthy * and
More informationValidation of HPTLC Method for Quantification of Embelin from Embelia ribes Burm. F.
Available online on www.ijppr.com International Journal of Pharmacognosy and Phytochemical Research 2016; 8(9); 1492-1496 Research Article ISSN: 0975-4873 Validation of HPTLC Method for Quantification
More informationAsian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:
Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: www.ajpamc.com ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF GEFITINIB
More informationCHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW
132 CHAPTER 6 DEVELOPMENT AND VALIDATION OF A STABILITY-INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF PARACETAMOL, TRAMADOL HYDROCHLORIDE AND DOMPERIDONE IN A COMBINED DOSAGE FORM 6.1 INTRODUCTION
More informationAnalysis of Counterfeit Antidiabetic Drugs by UHPLC with the Agilent 1220 Infinity Mobile LC
Analysis of Counterfeit Antidiabetic Drugs by UPLC with the Agilent 1 Infinity Mobile LC Application Note Small Molecule Pharmaceuticals & Generics Author Sonja Schneider Agilent Technologies, Inc. Waldbronn,
More information10 Kadam V. N. et al. Ijppr.Human, 2014; Vol. 1(2): 10-21 (Research Article) DEVELOPMENT AND VALIDATION OF ANALYTICAL METHODS FOR SIMULTANEOUS ESTIMATION OF VOGLIBOSE, GLIMEPIRIDE AND METFORMIN HYDROCHLORIDE
More informationA simple validated RP-HPLC method for quantification of sumatriptan succinate in bulk and pharmaceutical dosage form
IJPAR Vol.4 Issue 1 Jan-Mar-2015 Journal Home page: ISSN: 2320-2831 Research article Open Access A simple validated RP-HPLC method for quantification of sumatriptan succinate in bulk and pharmaceutical
More informationEffective use of Pharmacopeia guidelines to reduce cost of chromatographic analysis for Fluticasone propionate
Effective use of Pharmacopeia guidelines to reduce cost of chromatographic analysis for Fluticasone propionate Application Note Pharmaceutical QA/QC Author Siji Joseph Agilent Technologies, Inc. Bangalore,
More informationPelagia Research Library. Validated HPTLC Method for Simultaneous Estimation of Ramipril and Metolazone in Bulk Drug and Formulation
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2011, 2 (3): 230-238 ISSN: 0976-8688 CODEN (USA): PSHIBD Validated HPTLC Method for Simultaneous Estimation of Ramipril and Metolazone
More informationMethod Development and Validation for Simultaneous Estimation of Atorvastatin and Ezetimibe in Pharmaceutical Dosage Form by HPLC
World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.org/ Original
More informationRP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR CILOSTAZOL IN TABLET DOSAGE FORM
IJPAR Vol.5 Issue 4 Oct - Dec -2016 Journal Home page: ISSN:2320-2831 Research article Open Access RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR CILOSTAZOL IN TABLET DOSAGE FORM *1 M. Swapna *, 2 M.Vijay
More informationInternational Journal of Pharma and Bio Sciences
International Journal of Pharma and Bio Sciences RESEARCH ARTICLE PHARMACEUTICAL ANALYSIS DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR ESTIMATION OF ESCITALOPRAM OXALATE IN TABLET DOSAGE
More informationDEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF ALOGLIPTIN BENZOATE IN BULK DRUGS AND TABLET DOSAGE FORMS
DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF ALOGLIPTIN BENZOATE IN BULK DRUGS AND TABLET DOSAGE FORMS KOMAL SHARMA *1 & AMRITA PARLE 2 ABSTRACT A new, simple, precise, accurate and selective
More informationAN HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF RISPERIDONE AND TRIHEXYPHENIDYL HYDROCHLORIDE FROM BULK AND DOSAGE FORMS.
Research article Hygeia.J.D.Med.vol.3 (1), 2011, 29-33. www.hygeiajournal.com HYGEIA JOURNAL FOR DRUGS AND MEDICINES ISSN 2229 3590 (online) ISSN 0975 6221 (print) AN HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION
More informationDevelopment and Validation of RP-HPLC Method for the Estimation of Gemigliptin
Human Journals Research Article September 2018 Vol.:13, Issue:2 All rights are reserved by Hajera Khan et al. Development and Validation of RP-HPLC Method for the Estimation of Gemigliptin Keywords: Gemigliptin,
More informationDevelopment and validation of RP-HPLC method for simultaneous estimation of gliclazide and metformin in pure and tablet dosage form
ISSN: 2320-2831 IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: Research article Open Access Development and validation of RP-HPLC method for simultaneous estimation of gliclazide and metformin in
More informationIJRPC 2013, 3(2) Jajam Thriveni et al. ISSN: INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ROSUVASTATIN CALCIUM
More informationDevelopment and validation of analytical methods for estimation of imatinib mesylate in bulk and solid dosage forms by UV spectroscopy
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2015, 7 (3):214-220 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationVALIDATED HPLC METHOD FOR SIMULTANEOUS QUANTITATION OF TADALAFIL AND DAPOXETINE HYDROCHLORIDE IN BULK DRUG AND FORMULATION
Academic Sciences International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 0975-1491 Vol 4, Issue 2, 2012 Research Article VALIDATED HPLC METHD FR SIMULTANEUS QUANTITATIN F TADALAFIL AND DAPXETINE
More informationDetermination of Tetracyclines in Chicken by Solid-Phase Extraction and High-Performance Liquid Chromatography
Determination of Tetracyclines in Chicken by Solid-Phase Extraction and High-Performance Liquid Chromatography Application ote Food Safety Authors Chen-Hao Zhai and Yun Zou Agilent Technologies Co. Ltd.
More informationAvailable online Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2015, 7(9):951-960 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Analytical method development and validation for
More informationDevelopment and Validation for Simultaneous Estimation of Sitagliptin and Metformin in Pharmaceutical Dosage Form using RP-HPLC Method
International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.5, No.4, pp 1736-1744, Oct-Dec 2013 Development and Validation for Simultaneous Estimation of Sitagliptin and Metformin
More informationInternational Journal of Pharma and Bio Sciences DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF STRONTIUM RANELATE IN SACHET
International Journal of Pharma and Bio Sciences RESEARCH ARTICLE ANALYTICAL CHEMISTRY DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF STRONTIUM RANELATE IN SACHET K.MYTHILI *, S.GAYATRI,
More informationANALYTICAL TECHNIQUES FOR THE QUALITY OF ANTI- AIDS DRUGS
Research Article N. Bala Krishna, IJPRBS, 2013; Volume 2(1): 63-77 ISSN: 2277-8713 IJPRBS N.BALAA KRISHNA, Dr. M.V.V NAGESWARA REDDY IJPRBS-QR CODE PAPER-QR CODE DEVELOPMENT OF NEW ANALYTICAL TECHNIQUES
More informationInternational Journal of Research in Pharmaceutical and Nano Sciences Journal homepage:
Research Article CODEX: IJRPJK ISSN: 2319 9563 International Journal of Research in Pharmaceutical and Nano Sciences Journal homepage: www.ijrpns.com HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2015, 6(4): 30-37 ISSN: 0976-8688 CODEN (USA): PSHIBD Development and validation of stability indicating RP-HPLC method for simultaneous
More information36 J App Pharm Vol. 6; Issue 1: 36-42; January, 2014 Rao et al., 2014
36 J App Pharm Vol. 6; Issue 1: 36-42; January, 2014 Rao et al., 2014 Original Research Article SIMPLE AND RAPID LIQUID CHROMATOGRAPHIC METHOD FOR REAL-TIME QUANTIFICATION OF NAPROXEN / ESOMEPRAZOLE MAGNESIUM
More informationRao and Gowrisankar: Stability-indicating RP-HPLC Method for Pseudoephedrine, Ambroxol and Desloratadine
Research Paper Development and Validation of Stability-indicating RP- HPLC Method for the Estimation of Pseudoephedrine, Ambroxol and Desloratadine in Bulk and Tablet Dosage Forms N. MALLIKARJUNA RAO*
More informationStability indicating thin-layer chromatographic determination of eslicarbazepine acetate as bulk drug: Application to forced degradation study
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (11):38-47 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationDevelopment, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC Method
ISSN: 0973-4945; CODEN ECJAO E- Chemistry http://www.e-journals.net 2012, 9(1), 340-344 Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by PLC Method V.
More informationJ Pharm Sci Bioscientific Res (4): ISSN NO
Development and Validation of Analytical Methods for Simultaneous Estimation of Pregabalin and Amitriptyline Hydrochloride in their Combined Marketed Dosage form ABSTRACT: Nikhilkumar Patel, Gurjit Kaur,
More informationHyderabad, India. Department of Pharmaceutical Chemistry, Glocal University, Saharanpur, India.
International Journal On Engineering Technology and Sciences IJETS RP-HPLC Method development and validation for the Simultaneous Estimation of Metformin and Empagliflozine in Tablet Dosage Form Shaik
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research J. Chem. Pharm. Res., 2010, 2(5): 45-51 ISSN No: 0975-7384 CODEN(USA): JCPRC5 Simultaneous U.V. Spectrophotometric estimation
More informationRP-HPLC Method Development and Validation of Abacavir Sulphate in Bulk and Tablet Dosage Form
RP-HPLC Method Development and Validation of Abacavir Sulphate in Bulk and Tablet Dosage Form S. LAVANYA* 1, SK. MANSURA BEGUM 1, K. NAGAMALLESWARA RAO 2, K. GAYATHRI DEVI 3 Department of pharmaceutical
More informationA NOVEL RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF BERBERINE, QUERCETIN, AND PIPERINE IN AN AYURVEDIC FORMULATION
International Journal of Applied Pharmaceutics ISSN- 0975-7058 Vol 11, Issue 1, 2019 Original Article A NOVEL RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF BERBERINE, QUERCETIN, AND PIPERINE IN AN AYURVEDIC
More informationDEVELOPMENT OF RP-HPLC METHOD FOR ESTIMATION OF DROTAVERINE HYDROCHLORIDE IN PHARMACEUTICAL FORMULATIONS
Int. J. Chem. Sci.: 6(4), 2008, 2055-2061 DEVELOPMENT OF RP-HPLC METHOD FOR ESTIMATION OF DROTAVERINE HYDROCHLORIDE IN PHARMACEUTICAL FORMULATIONS B. S. SASTRY a, S. GANANADHAMU and G. DEVALA RAO K. V.
More informationDetermination of β2-agonists in Pork Using Agilent SampliQ SCX Solid-Phase Extraction Cartridges and Liquid Chromatography-Tandem Mass Spectrometry
Determination of β2-agonists in Pork Using Agilent SampliQ SCX Solid-Phase Extraction Cartridges and Liquid Chromatography-Tandem Mass Spectrometry Application Note Food Safety Authors Chenhao Zhai Agilent
More informationVol-3, Issue-4, Suppl-1, Nov 2012 ISSN: Shingala et al PHARMA SCIENCE MONITOR
PHARMA SCIENCE MONITOR AN INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF GALLIC ACID AND ANDROGRAPHOLIDE IN POLYHERBAL FORMULATION Dhara Shingala*,
More informationEstimation of zolmitriptan by a new RP-HPLC method
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2012, 4 (3):1022-1026 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationScholars Research Library. Der Pharmacia Lettre, 2015, 7 (5):44-49 (
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2015, 7 (5):44-49 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR DETERMINATION OF ROSUVASTATIN CALCIUM IN BULK AND PHARMACEUTICAL DOSAGE FORM
Research Article DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR DETERMINATION OF ROSUVASTATIN CALCIUM IN BULK AND PHARMACEUTICAL DOSAGE FORM Chirag B. Pandya*, K.P. Channabasavaraj, Jaydeep D. Chudasama,
More informationReverse Phase HPLC Analysis of Atomoxetine in Pharmaceutical Dosage Forms
Asian Journal of Chemistry Vol. 21, No. 2 (2009), 829-833 Reverse Phase HPLC Analysis of Atomoxetine in Pharmaceutical Dosage Forms B.V.V.S. JAGADEESH, S. SATYANARAYANA RAJU, V.JAYATHIRTHA RAO and J.V.L.N.
More informationCorresponding Author:
Adv J Pharm Life sci Res, 2017 5;3:1-8 ISSN 2454 3535 (On-line) RP-HPLC Method for Estimation of Mupirocin in Bulk and Pharmaceutical Formulation S.K.Attar 1 *, M.S.Kalshetti 2, N. A. Jadhao 3, N. R. Patel
More informationJournal of Pharmacreations
Journal of Pharmacreations ISSN: 2348-6295 Pharmacreations Vol.5 Issue 1 Jan- Mar- 2018 Journal Home page: www.pharmacreations.com Research article Open Access Method development and validation of apixaban
More informationSimultaneous estimation of Metformin HCl and Sitagliptin in drug substance and drug products by RP-HPLC method
International Journal of Chemical and Pharmaceutical Sciences 2017, Mar., Vol. 8 (1) ISSN: 0976-9390 IJCPS Simultaneous estimation of Metformin HCl and Sitagliptin in drug substance and drug products by
More informationScholars Research Library
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2010, 2(5): 363-370 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationAnalytical method validation for tablet of phenoxymethyl penicillin potassium by RP-HPLC method
Analytical method validation for tablet of phenoxymethyl penicillin potassium by RP-HPLC method Baghel U.S.* 1, Singhal M. 2, Gupta M.M. 1, Singh H.P. 3, Dave Shuchi 4, Sahu M. *1 Jaipur College of Pharmacy,
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS OF TRAMADOL IN PURE AND PHARMACEUTICAL FORMULATIONS
Int. J. Chem. Sci.: 10(4), 2012, 2039-2047 ISSN 0972-768X www.sadgurupublications.com DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS OF TRAMADOL IN PURE AND PHARMACEUTICAL FORMULATIONS
More informationA Validated Chiral Liquid Chromatographic Method for The Enantiomeric Separation of Dapoxetine Hydrochloride
Received on 15/05/2012; Revised on 22/05/2012; Accepted on 09/06/2012 A Validated Chiral Liquid Chromatographic thod for The Enantiomeric Separation of Dapoxetine Hydrochloride T.Rohith 1 and S. Ananda
More informationINTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES
INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES Development of standardization of trikatu churna: A spectrophotometric approach Manjesh Thakur*, P. Porwal, P. Pal, K. Shukla and S.C. Mahajan Mahakal
More informationQUANTITATIVE ESTIMATION OF PIPERINE COUGH SYRUP BY HPTLC METHOD
IN HERBAL COUGH SYRUP BY HPTLC METHOD P. Shanmugasundaram, R. Maheswari, and M. Vijayaanandhi Department of Pharmaceutical Analysis, Vel s College of Pharmacy, Chennai-600117, Tamilnadu, India. Email:
More informationInternational Journal of Medicine and Pharmaceutical Research. International Journal of Medicine and Pharmaceutical Research
International Journal of Medicine and Pharmaceutical Research Journal Home Page: www.pharmaresearchlibrary.com/ijmpr Research Article Open Access Development and Validation of RP-HPLC Method for the Estimation
More informationRP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF BACLOFEN IN BULK AND PHARMACEUTICAL DOSAGE FORMS
Int. J. Chem. Sci.: 11(1), 2013, 390-398 ISSN 0972-768X www.sadgurupublications.com RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF BACLOFEN IN BULK AND PHARMACEUTICAL DOSAGE FORMS SAROJ
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(2):770-775 Validation of Rapid Liquid Chromatographic
More informationANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF DILTIAZEM HYDROCHLORIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM BY RP- HPLC
Research Communication ISSN 2394-2789(Print) e-issn 2394-2797(Online) ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF DILTIAZEM HYDROCHLORIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM BY
More informationQuantitative estimation of Rosuvastatin in bulk and tablet dosage form by using area under curve method
Original research article ISSN 2321-0125 www.jpbs-online.com Quantitative estimation of Rosuvastatin in bulk and tablet dosage form by using area under curve method P. S. Jain*, N. K. Kale, S. J. Surana
More informationScholars Research Library
Available online at www.derpharmachemica.com Scholars Research Library Der Pharma Chemica, 2010, 2(5): 46-52 (http://derpharmachemica.com/archive.html) ISSN 0975-413X CODEN (USA): PCHHAX Development and
More informationCHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW
51 CHAPTER 2 SIMULTANEOUS ESTIMATION OF PIOGLITAZONE, GLIMEPIRIDE AND GLIMEPIRIDE IMPURITIES IN COMBINATION DRUG PRODUCT BY A VALIDATED STABILITY-INDICATING RP-HPLC METHOD 2.1 INTRODUCTION OF DOSAGE FORM
More informationSIMULTANEOUS ESTIMATION OF LEVOCETIRIZINE DIHYDROCHLORIDE AND MONTELUKAST SODIUM BY RP-HPLC METHOD
SIMULTANEOUS ESTIMATION OF LEVOCETIRIZINE DIHYDROCHLORIDE AND MONTELUKAST SODIUM BY RP-HPLC METHOD 1 1 Sushma Somkuwar, A.K. Pathak * Department of Pharmacy, Barkatullah University, Bhopal, M.P., [India]
More informationAmudha S et al., Asian Journal of Pharmthiaceutical Technology & Innovation, 04 (21); 2016; Research Article
Asian Journal of Pharmaceutical Technology & Innovation ISSN: 2347-8810 Research Article Received on: 09-11-2016 Accepted on: 20-11-2016 Published on: 15-12-2016 Corresponding Author: * Amudha S, Dept.
More informationSIMPLE AND VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF CARBOPLATIN IN BULK AND FORMULATION DOSAGE FORM. Subhashini.Edla* B.
SIMPLE AND VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF CARBOPLATIN IN BULK AND FORMULATION DOSAGE FORM Subhashini.Edla* B.Syama sundhar Abstract Dept of Chemistry, Acharya Nagarjuna University, Nagarjuna
More information