Original Article A Validated RP-HPLC Method for Quantification of Gallic Acid as marker in Different Extracts of Symplocos racemosa (Roxb)

Size: px
Start display at page:

Download "Original Article A Validated RP-HPLC Method for Quantification of Gallic Acid as marker in Different Extracts of Symplocos racemosa (Roxb)"

Transcription

1 Available online at International Journal of Chromatographic Science Universal Research Publications. All rights reserved Original Article A Validated RP-HPLC Method for Quantification of Gallic Acid as marker in Different Extracts of Symplocos racemosa (Roxb) Name of contributors Nagore Dheeraj H 1. *- M. Pharm (JJT University, Jhunjhunu, Rajasthan, India) Kuber Vinod V 2. PhD (Analytical Department, Tulip Lab Pvt. Ltd, Pune) Patil Pankaj S 2. M. Pharm (Analytical Department, Tulip Lab Pvt. Ltd, Pune) Sagulale Amol D 2. M. Pharm (Analytical Department, Tulip Lab Pvt. Ltd, Pune) Deshmukh Tushar A 3. PhD (Pharmacognosy Department, College of Pharmacy Faizpur, Jalgaon) ID nagoredheeraj@gmail.com Conflicting Interest - There is no Conflicting Interest in this manuscript. Received 21 September 2012; accepted 08 October 2012 Abstract Applications of modern analytical techniques are important for the quality evaluation and standardization of Herbal extracts. Symplocos racemosa (Roxb), an important medicinal plant with wide medicinal properties, is frequently used in various traditional herbal preparations. Gallic acid, major bioactive tannin was selected as chemical marker of S. racemosa (Roxb), and RP- HPLC method was developed for the quantification of Gallic acid using Inertsil C8-4 (250 x 4.6) mm, 5u column. The detection was carried out at wavelength 280 nm. The Gallic acid was satisfactorily resolved with Retention time (R t ) value about 8.5 minutes. The linear regression analysis data for the calibration plots showed good linear relationship with r 2 = The % RSD values for system precision, method precision and intermediate precision was found to 0.26 %, 0.31 % and 0.59 % respectively. According to the International Conference on Harmonization (ICH) guidelines, the method was validated in terms of its Linearity, specificity, recovery, precision, robustness and solution stability by following standard protocols. Statistical analysis of the data showed that the method is reproducible and selective for the determination of Gallic acid. The method was able to identify and quantify Gallic acid from complex mixtures of phytochemicals containing S. racemosa Universal Research Publications. All rights reserved Keywords: Symplocos racemosa (Roxb), Gallic acid, RP-HPLC, Standardization. 1. Introduction In Traditional Indian Medicinal System (TIMS) Ayurveda has been practiced in India since ancient times. Ayurveda is plant based system of medicine which already gained worldwide attention due to its safety and efficacy because it is based on philosophical, experiential and experimental data [1]. In the Ayurveda, various Formulations containing medicinal plants for the treatment of various diseases and its complications are included. Now a days, lack of sexual desire and erectile dysfunction has becomes the important problem in human society. Lodhra (Symplocos racemosa Roxb.) is one of the imperative plants reported to posses aphrodisiac property. It is reported to have the phosphodiesterase inhibitor activity and can be used in erectile dysfunction [2]. As per Ayurvedic literature Lodhra is also useful for erectile dysfunction, anemia, skin disorders, gastrointestinal problems etc. It belongs to family Symplocaceae [3]. Unani medicine uses it as an emmenagogue and aphrodisiac. It is widely used not only for gynecological disorders but is also used as a potent remedy for inflammation and cleaning of the uterus. This is used to treat leucorrhoea and menorrhagia [4]. Its decoction is also used for the treatment of bowel complaints and ulcers [5]. Medicinally bark is useful in eye diseases, blood purification, leprosy, dropsy and liver complaints [4]. A large number of chemical compounds belonging to different classes such as alkaloids, diterpenoid lactones, glycosides, phenolics compounds are reported in Lodhra. Bark contains flavanol glucosides like symplocoside, symposide, leucopelargonidin 3-glucoside, ellagic acid, flavonol glycoside like rhamnetin 3-digalactoside, triterpenoids like 19α-hydroxyarjunolic acid-3,28-o-bis-βglucopyranosides, 19α-hydroxyasiatic acid-3, 28-O-bis-βglucopyranosides, betulin, Oleanolic acid, β- sitosterol and α-amyrin, alkaloids loturine, isoloturine and harmane [6]. Apart from these chemical constituents the bark mainly contains Gallic Acid [7, 8]. Many formulation of Lodhra are also reported in Ayurveda like Rodharasava, Pusyanuga Churna, Gangadhara Churna etc [9]. One of important activity for achieving standardization is availability/ development of marker based analytical 19

2 method to analyse raw material. As per literature review, although Lodhra is a important plant in Ayurveda, still very few analytical methods like HPTLC of (-) epiafzelechin [9] β sitosterol [10] and Ellagic acid [11] are reported. These compounds are more or less ubiquitous. Gallic Acid is specific tannin present in Lodhra and hence it can be used to discriminate the Lodhra from other genus and species. In the present study, efforts have been made to standardize Lodhra by developing RP HPLC method for the quantitation of Gallic acid. 2. Experimental 2.1. Plant Material The bark of Symplocos racemosa Roxb. were collected from Pune regions of Maharashtra and authenticate by botanical survey of India, Pune [Voucher Specimen No.SRDN001]. The powdered bark was passed through sieve no. 85, weighed and then used for extraction Preparation of Extract Powdered bark was extracted with water, alcohol and water-alcohol mixture (1:1) separately for 72 hours at 80 o C by using soxhlet apparatus. These extracts were then concentrated to dryness by removing the solvent in the rotary evaporator under reduced pressure. The polar solvents were used for extraction because Symplocos racemosa (Roxb) contains maximum amount of polar constituents. Also Gallic acid is easily soluble in polar solvent. The validation of the method was carried out on Alcoholic extract Determination of Percent Yield of Extract The percent yield of extract obtained from all the extracts were calculated from the following equation: Percent Yield = (W1 100) / W2 Where, W1 was the weight of the extract after the solvent evaporation and W2 was the weight of powdered bark taken Chemicals HPLC grade solvents like acetonitrile and methanol were obtained from Merck Ltd, India. Standard Gallic acid [Potency % Product no.27645] was purchased from Sigma, Bangalore India Chromatographic Conditions for HPLC High Performance Liquid Chromatography method was performed with Waters 2695 Alliance system with a 2996 photodiode array detector (PDA). Gallic acid was separated on a reverse-phase 250 mm 4.6 mm, 5μ, Inertsil C8-4 column (LCGC). The mobile phase was prepared from 0.1 % orthophosphoric acid in water of ph 2.5 (solvent A) and acetonitrile (solvent B). The mobile phase was degassed and filtered through 0.45-μm filter before use. The gradient program used was : Initial 0 10 min, from A B (95:5 v/v), min, linear change from A B (95:5 v/v) to A B (5:95 v/v), min, constant change from A B (5:95 v/v), on min linear changes from A B (5:95 v/v) to A B (95-5 v/v), min, constant change from A B (95-5 v/v). The mobile phase flow rate was 1 ml min 1. Before the first injection, the column was saturated for 30 minutes with the initial mobile phase. The column temperature was maintained at 30 C. The injection volume was kept 10 μl. The PDA was set at 280 nm to acquire the chromatogram. The Gallic acid was identified by comparing the retention time and spectra obtained from sample and standard solutions. The present work was performed in an airconditioned room maintained at 22 o C Preparation of Standard Solution of Gallic acid A stock solution of Gallic acid was prepared by dissolving 100 mg of standard in 100 ml methanol [1000 µg/ml]. 1 ml of stock solution was diluted up to 10 ml to make the 100 µg/ml solution and was used as working standard for the analysis Preparation of Test Solution for Analysis. 1 gm extract was accurately weighed. To this 80 ml of water and 1 ml of concentrated hydrochloric acid AR grade was added and it was kept on reflux for 2 hour on water bath at 100 o C. Mixture was allowed to cool at room temperature and diluted up to 100 ml with water. The mixture was filtered and allowed to evaporate on water bath. The residue was dissolved in 20 ml methanol. This resulting solution was used as test solution Preparation of Calibration graph. The stock solution of Gallic Acid was diluted to seven different concentrations between % of working concentration. These were injected in triplicate for the preparation of calibration graph. The calibration graph was plotted by using the concentrations versus average peak area at 280 nm Validation of HPLC Method The proposed HPLC method was validated in terms of precision, specificity, linearity, accuracy, solution stability and robustness as per the ICH guidelines [12] Specificity The specificity of the method was studied by assessment of peak purity of Gallic acid using Waters empower software and diode array detector [Figure 5] and represented in terms of purity angle, purity threshold and purity flag [Table 5] Precision System Precision The repeatability of the sample application and measurement of peak area were carried out by spotting seven replicate spots of standard and was expressed in terms of percent relative standard deviation (% RSD) [Table 1] Method Precision Seven different samples of extract were prepared separately and analysed as per the method. The percent assay was calculated and the precision was expressed in terms of % RSD [Table 1] Intermediate Precision The intermediate precision was determined by analyzing sample solution by two different analysts on different day using different set of instruments. The percent assay was calculated and the precision was expressed in terms of % RSD [Table 1] Solution Stability The sample solution and standard solution were prepared as per the proposed method and subjected to stability study at room temperature for 14 h. The sample solution was analyzed at initial and at different time intervals up to 14 hrs. The change in response of Gallic acid in sample solution with respect to time is calculated as absolute 20

3 percent difference against initial response [Table 1]. Table 1 Method validation parameters for quantitation of Gallic acid Sr. No. Parameters HPLC 1. Linearity ( correlation coefficient r 2 ) System precision. (% RSD) (n=6) Method Precision. (% RSD) (n=6) Intermediate precision. (% RSD) (n=6) Solution stability Stable Robustness The robustness of the method was determined by slight deviation in the method parameters. The parameters selected were deviation in the wavelength, column temperature, flow rate and mobile phase gradient. The Retention time of Gallic acid was determined and % RSD with system suitability parameters was observed [Table 2]. Table 2 Robustness of the method Sr. No. Parameters for HPLC % RSD 1 Wavelength Column Temperature Flow Rate Mobile Phase Gradient Studies The accuracy of the method was determined from recovery studies by adding known amount of standards at 80, 100 and 120% level to the preanalyzed sample followed by replicate quantitative analyses by the proposed method for three times [Table 3]. Table 3 study Sr. no level Amount added (mg) Amount recovered (mg) % 80% % % % % % % % % % Average Analysis of Extracts The content of Gallic acid in water, alcoholic and wateralcoholic extract was determined as per the method described under chromatographic conditions. All the analysis was repeated three times and average values are mentioned in Table 4. Table 4 Analysis of Gallic acid from Extracts Sr. No. Name of Extract Assay (%w/w) 1 Water extract of bark of Lodhra Alcohol extract of bark of Lodhra Water-alcohol extract of bark of Lodhra Results 3.1. Estimation of Percent Yield of Extract The percent yield of water, alcoholic and water-alcoholic extracts of Lodhra bark were found to be % w/w, % w/w and % w/w respectively. Table 5 Specificity Parameters Sr Purity Purity Purity Standard No Angle Threshold Flag Gallic No Flag acid found * No Flag found* means no interference in Gallic acid peak Figure 1 Chromatogram of Standard Gallic acid Figure 2 Chromatogram of Water Extract of Lodhra 3.2. Chromatographic Study The composition of mobile phase in HPLC method was optimized by testing different solvent compositions of varying polarity, column chemistry and the best results were obtained by using present method which produces highly symmetrical peaks showing good resolution between Gallic acid and other peaks [Figure 1]. The scanning wavelength selected was 280 nm for Gallic acid. At this wave length the Gallic acid showed optimum response [Figure 2-4]. The Gallic acid was satisfactorily resolved with Retention time (R t ) value about 8.5 minutes. Figure 3 Chromatogram of Alcohol Extract of Lodhra. The calibration graph is linear in the working range of µg/ml with acceptable correlation coefficient [Table 1]. The graph for standard Gallic acid is given in Figure 6. 21

4 Figure 4 Chromatogram of Water-Alcohol Extract of Lodhra Figure 5 Peak purity spectra of Gallic acid for Specificity Figure 6 Linearity graph for Gallic acid The values of system precision, method precision and intermediate precision are given against sample application and scanning of peak area and expressed in terms of %RSD. The values were found to be 0.26 %, 0.31 % and 0.59 % respectively for system precision, method precision and intermediate precision, the % RSD values depicted in Table 1 showed that the proposed method provides acceptable level of system precision, method precision and intermediate precision. The peak purity of Gallic acid was assessed by comparing their respective spectra at peak start, peak apex, and peak end positions of the spot from standard and extracts [Figure 5]. Purity angle and Purity threshold was found to be and respectively with no purity flag [Table 5]. The given method was optimized by doing robustness. The peak area of Gallic acid was calculated for each parameter and % RSD was found to be less than 2%. The values of %RSD as shown in Table 2 indicated better robustness of the method. The proposed method was used for estimation of Gallic acid from extract after spiking with 80, 100 and 120% of additional standard of Gallic acid to preanalysed sample. The recovery percent of Gallic acid was found to be 98.49, and [Table 3] Analysis of Gallic acid from Extracts The content of Gallic acid in water, alcoholic and wateralcoholic extracts were found to be 0.67, 0.60 and 0.75 %w/w and reported in Table 4. The results showed interesting differences in the amounts of Gallic acid present in different extracts of same plant. 4. Discussion HPLC is a powerful analytical technique for the herbal drug analysis. This method has accuracy, sensitivity, reproducibility, resolution and automation. The method also provides nanogram sensitivity, adequate linearity and repeatability [13]. The method is precise for sample application and measurement of peak areas with low values of % R.S.D. suggested an excellent precision of the method. The results obtained after recovery study showed the method is accurate for the analysis of gallic acid from Lodhra bark extract. The method reported here is rapid and suitable for the quantitation of Gallic acid from the bark extract of Lodhra. Gallic acid can easily be quantified in the presence of other constituents from the extract of Lodhra bark without compromising the accuracy. 5. Conclusion The developed HPLC technique is precise, specific, accurate and robust for the determination of Gallic acid. The proposed method can be used for qualitative as well as quantitative analysis of Gallic acid from extract of bark of Symplocos racemosa (Roxb.) References: 1. J.M. Humber, The role of complementary and alternative medicine: accommodating pluralism, J. Am. Med. Assoc. 288 (2002) V.U. Ahmad, A.A. Muhammad, H. Hussain, N.A. Muhammad, U. Farooq, N. Fatima, I.M. Choudhary, Phenolic glycosides from Symplocos racemosa: natural inhibitors of phosphodiesterase-i, Phytochemistry. 63 (2003) Anonymous, The Ayurvedic Pharmacopoeia of India. New Delhi: Controller of publications, Government of India, 1 (1999) K.K. Bhutani, A.N. Jadhav, V. Kalia, Effect of Symplocos racemosa Roxb. on gonadotropin release in immature female rats and ovarian histology, J. Ethnopharmacol. 94 (2004) R. Dhaon, G.K. Jain, J.P.S. Sarin, N.M. Khanna, Symposide: a new anti fibrinolytic glycoside from Symplocos racemosa Roxb., Ind. J. Chem. 28B (1989) R. Badonia, D.K. Semwalb, S.K. Kothiyala, U. Rawat, Chemical constituents and biological applications of the genus Symplocos, J. Asian. Nat. Prod. Res. 2 (2010) K.R. Kritikar, B.D. Basu, Indian Medicinal Plants. International Book Distributors, second ed., Dehradun, (1935) I. Junko, M.W. Oyama, L. Mark, L. Kuo-Hsiung, Anti- AIDS Agents Anti-HIV Activity of Harman, an 22

5 9. Anti-HIV Principle from Symplocos setchuensis, and Its Derivatives, J. Nat. Prod. 64 (2001) Anonymous. Quality standards of Indian Medicinal Plants (Indian council of medical research), fourth ed., New Delhi, (2005) S. Shah, S. hailaja, High Performance Thin Layer Chromatographic Quantification of b- Sitosterol from stem bark of Symplocos racemosa Roxb. and it s formulation, J. Herbal. Med. Toxicol.4 (2010) R. Rao, B. Bhavya, K. Pavani, A. Swapna, C.H. Prasoona, Anthelmintic activity of Symplocos racemosa, Int. J. Pharm. Biol. Sci. 1 (2011) International Conference on the Harmonization of Technical Requirements for the Registration of Pharmaceuticals for Human Use (ICH) Q2B.Validation of Analytical Procedures, Methodology. (1996). 14. S. Chopra, J.A. Farhan, R.K. Khar, K.S. Motwani, S. Mahdi, S. Talegaonkar, Validated high-performance thin-layer chromatography method for determination of trigonelline in herbal extract and pharmaceutical dosage form, Anal. Chim. Acta. 557 (2006) Source of support: Nil; Conflict of interest: None declared 23

Development and Validation of Improved RP-HPLC method for Identification and Estimation of Ellagic and Gallic acid in Triphala churna

Development and Validation of Improved RP-HPLC method for Identification and Estimation of Ellagic and Gallic acid in Triphala churna International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol.2, No.3, pp 1486-1493, July-Sept 2010 Development and Validation of Improved RP-HPLC method for Identification and Estimation

More information

Amudha S et al., Asian Journal of Pharmthiaceutical Technology & Innovation, 04 (21); 2016; Research Article

Amudha S et al., Asian Journal of Pharmthiaceutical Technology & Innovation, 04 (21); 2016; Research Article Asian Journal of Pharmaceutical Technology & Innovation ISSN: 2347-8810 Research Article Received on: 09-11-2016 Accepted on: 20-11-2016 Published on: 15-12-2016 Corresponding Author: * Amudha S, Dept.

More information

Simultaneous Estimation of Gemcitabine Hydrochloride and Capecitabine Hydrochloride in Combined Tablet Dosage Form by RP-HPLC Method

Simultaneous Estimation of Gemcitabine Hydrochloride and Capecitabine Hydrochloride in Combined Tablet Dosage Form by RP-HPLC Method ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2011, 8(3), 1212-1217 Simultaneous Estimation of Gemcitabine Hydrochloride and Capecitabine Hydrochloride in Combined Tablet Dosage

More information

RP-HPLC Analysis of Temozolomide in Pharmaceutical Dosage Forms

RP-HPLC Analysis of Temozolomide in Pharmaceutical Dosage Forms Asian Journal of Chemistry Vol. 22, No. 7 (2010), 5067-5071 RP-HPLC Analysis of Temozolomide in Pharmaceutical Dosage Forms A. LAKSHMANA RAO*, G. TARAKA RAMESH and J.V.L.N.S. RAO Department of Pharmaceutical

More information

Development and validation of stability indicating RP-LC method for estimation of calcium dobesilate in pharmaceutical formulations

Development and validation of stability indicating RP-LC method for estimation of calcium dobesilate in pharmaceutical formulations Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (11):236-242 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4

More information

Journal of Chemical and Pharmaceutical Research, 2013, 5(1): Research Article

Journal of Chemical and Pharmaceutical Research, 2013, 5(1): Research Article Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2013, 5(1):180-184 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 A simple and sensitive RP-HPLC method for estimation

More information

Stability indicating RP-HPLC method development and validation of Etizolam and Propranolol hydrochloride in pharmaceutical dosage form

Stability indicating RP-HPLC method development and validation of Etizolam and Propranolol hydrochloride in pharmaceutical dosage form World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.org/ Original

More information

CHAPTER 8 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS OF M. ROXBURGHIANUS AND P. FRATERNUS PLANT EXTRACTS

CHAPTER 8 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS OF M. ROXBURGHIANUS AND P. FRATERNUS PLANT EXTRACTS CHAPTER 8 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS OF M. ROXBURGHIANUS AND P. FRATERNUS PLANT EXTRACTS CHAPTER 8: HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

More information

METHOD DEVELOPMENT AND VALIDATION BY RP-HPLC FOR ESTIMATION OF ZOLPIDEM TARTARATE

METHOD DEVELOPMENT AND VALIDATION BY RP-HPLC FOR ESTIMATION OF ZOLPIDEM TARTARATE WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES Ramalakshmi et al. SJIF Impact Factor 6.647 Volume 7, Issue 2, 1010-1018 Research Article ISSN 2278 4357 METHOD DEVELOPMENT AND VALIDATION BY RP-HPLC

More information

CHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW

CHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW 132 CHAPTER 6 DEVELOPMENT AND VALIDATION OF A STABILITY-INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF PARACETAMOL, TRAMADOL HYDROCHLORIDE AND DOMPERIDONE IN A COMBINED DOSAGE FORM 6.1 INTRODUCTION

More information

Pelagia Research Library

Pelagia Research Library Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2014, 5(5):91-98 ISSN: 0976-8688 CODEN (USA): PSHIBD A novel RP-HPLC method development and validation of Perindopril Erbumine in

More information

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD ESTIMATION OF TOLVAPTAN IN BULK PHARMACEUTICAL FORMULATION

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD ESTIMATION OF TOLVAPTAN IN BULK PHARMACEUTICAL FORMULATION http://www.rasayanjournal.com Vol.4, No.1 (2011), 165-171 ISSN: 0974-1496 CODEN: RJCABP DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR AND ITS PHARMACEUTICAL FORMULATION V. Kalyana Chakravarthy * and

More information

ISSN (Print)

ISSN (Print) Scholars Academic Journal of Pharmacy (SAJP) Sch. Acad. J. Pharm., 2014; 3(3): 240-245 Scholars Academic and Scientific Publisher (An International Publisher for Academic and Scientific Resources) www.saspublisher.com

More information

RP-HPLC Method Development and Validation of Abacavir Sulphate in Bulk and Tablet Dosage Form

RP-HPLC Method Development and Validation of Abacavir Sulphate in Bulk and Tablet Dosage Form RP-HPLC Method Development and Validation of Abacavir Sulphate in Bulk and Tablet Dosage Form S. LAVANYA* 1, SK. MANSURA BEGUM 1, K. NAGAMALLESWARA RAO 2, K. GAYATHRI DEVI 3 Department of pharmaceutical

More information

Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC Method

Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC Method ISSN: 0973-4945; CODEN ECJAO E- Chemistry http://www.e-journals.net 2012, 9(1), 340-344 Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by PLC Method V.

More information

Reverse Phase HPLC Analysis of Atomoxetine in Pharmaceutical Dosage Forms

Reverse Phase HPLC Analysis of Atomoxetine in Pharmaceutical Dosage Forms Asian Journal of Chemistry Vol. 21, No. 2 (2009), 829-833 Reverse Phase HPLC Analysis of Atomoxetine in Pharmaceutical Dosage Forms B.V.V.S. JAGADEESH, S. SATYANARAYANA RAJU, V.JAYATHIRTHA RAO and J.V.L.N.

More information

Development and validation of related substances method for Varenicline and its impurities

Development and validation of related substances method for Varenicline and its impurities Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (1):304-309 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4

More information

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS TOLBUTAMIDE IN PURE AND PHARMACEUTICAL FORMULATIONS

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS TOLBUTAMIDE IN PURE AND PHARMACEUTICAL FORMULATIONS Int. J. Chem. Sci.: 11(4), 2013, 1607-1614 ISSN 0972-768X www.sadgurupublications.com DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS TOLBUTAMIDE IN PURE AND PHARMACEUTICAL FORMULATIONS

More information

Simultaneous estimation of Metformin HCl and Sitagliptin in drug substance and drug products by RP-HPLC method

Simultaneous estimation of Metformin HCl and Sitagliptin in drug substance and drug products by RP-HPLC method International Journal of Chemical and Pharmaceutical Sciences 2017, Mar., Vol. 8 (1) ISSN: 0976-9390 IJCPS Simultaneous estimation of Metformin HCl and Sitagliptin in drug substance and drug products by

More information

Simultaneous Determination of Halobetasol and Salicylic Acid Related Substances by Reversed Phase High Performance Liquid Chromatographic Method

Simultaneous Determination of Halobetasol and Salicylic Acid Related Substances by Reversed Phase High Performance Liquid Chromatographic Method Asian Journal of Chemistry Vol. 22, No. 5 (2010), 3369-3375 Simultaneous Determination of Halobetasol and Salicylic Acid Related Substances by Reversed Phase High Performance Liquid Chromatographic Method

More information

J Pharm Sci Bioscientific Res (4): ISSN NO

J Pharm Sci Bioscientific Res (4): ISSN NO Development and Validation of Analytical Methods for Simultaneous Estimation of Pregabalin and Amitriptyline Hydrochloride in their Combined Marketed Dosage form ABSTRACT: Nikhilkumar Patel, Gurjit Kaur,

More information

Scholars Research Library. Der Pharmacia Lettre, 2016, 8 (6): (http://scholarsresearchlibrary.com/archive.html)

Scholars Research Library. Der Pharmacia Lettre, 2016, 8 (6): (http://scholarsresearchlibrary.com/archive.html) Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (6):217-223 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4

More information

Journal of Chemical and Pharmaceutical Research

Journal of Chemical and Pharmaceutical Research Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(1):138-144 Simultaneous RP HPLC determination of Latanoprost

More information

Volume 2 (6), 2014, Page CODEN (USA)-IJPRUR, e-issn: International Journal of Pharma Research and Health Sciences

Volume 2 (6), 2014, Page CODEN (USA)-IJPRUR, e-issn: International Journal of Pharma Research and Health Sciences CODEN (USA)-IJPRUR, e-issn: 2348-6465 International Journal of Pharma Research and Health Sciences Available online at www.pharmahealthsciences.net Original Article Simultaneous Determination of Salbutamol

More information

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF LACOSAMIDE IN BULK AND ITS PHARMACEUTICAL FORMULATION

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF LACOSAMIDE IN BULK AND ITS PHARMACEUTICAL FORMULATION http://www.rasayanjournal.com Vol.4, No.3 (2011), 666-672 ISSN: 0974-1496 CODEN: RJCABP DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR IN BULK AND ITS PHARMACEUTICAL FORMULATION V.Kalyan Chakravarthy*

More information

HPTLC Determination of Atomoxetine Hydrochloride from its Bulk Drug and Pharmaceutical Preparations

HPTLC Determination of Atomoxetine Hydrochloride from its Bulk Drug and Pharmaceutical Preparations Asian Journal of Chemistry Vol. 20, No. 7 (2008), 5409-5413 HPTLC Determination of Atomoxetine Hydrochloride from its Bulk Drug and Pharmaceutical Preparations S.S. KAMAT, VINAYAK T. VELE, VISHAL C. CHOUDHARI

More information

CHAPTER 2 SIMULTANEOUS DETRMINATION OF ANASTROZOLE AND TEMOZOLOMIDE TEMOZOLOMIDE CAPSULES 20 MG AND ANASTROZOLE TABLETS 1 MG

CHAPTER 2 SIMULTANEOUS DETRMINATION OF ANASTROZOLE AND TEMOZOLOMIDE TEMOZOLOMIDE CAPSULES 20 MG AND ANASTROZOLE TABLETS 1 MG CHAPTER 2 SIMULTANEOUS DETRMINATION OF ANASTROZOLE AND TEMOZOLOMIDE IN TEMOZOLOMIDE CAPSULES 20 MG AND ANASTROZOLE TABLETS 1 MG ANALYTICAL METHOD VALIDATION REPORT FOR ASSAY 43 2.1 Introduction Analytical

More information

Sanjog Ramdharane 1, Dr. Vinay Gaitonde 2

Sanjog Ramdharane 1, Dr. Vinay Gaitonde 2 JPSBR: Volume 5, Issue 2: 2015 (151-155) ISS. 2271-3681 A ew Gradient RP- HPLC Method for Quantitative Analysis of : (3-luoro-4- Morpholin-4-yl-Phenyl)-Carbamic Acid Methyl Ester and its Related Substances

More information

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS OF TRAMADOL IN PURE AND PHARMACEUTICAL FORMULATIONS

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS OF TRAMADOL IN PURE AND PHARMACEUTICAL FORMULATIONS Int. J. Chem. Sci.: 10(4), 2012, 2039-2047 ISSN 0972-768X www.sadgurupublications.com DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS OF TRAMADOL IN PURE AND PHARMACEUTICAL FORMULATIONS

More information

Method Development and Validation for the Estimation of Saroglitazar in Bulk and Pharmaceutical Dosage Form by RP-HPLC

Method Development and Validation for the Estimation of Saroglitazar in Bulk and Pharmaceutical Dosage Form by RP-HPLC Original Research Method Development and Validation for the Estimation of Saroglitazar in Bulk and Pharmaceutical Dosage Form by RP-HPLC Hanumantha Rao K 1, Lakshmana Rao A 2,*, Chandra Sekhar KB 3 1 Assistant

More information

International Journal of Pharma and Bio Sciences DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF STRONTIUM RANELATE IN SACHET

International Journal of Pharma and Bio Sciences DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF STRONTIUM RANELATE IN SACHET International Journal of Pharma and Bio Sciences RESEARCH ARTICLE ANALYTICAL CHEMISTRY DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF STRONTIUM RANELATE IN SACHET K.MYTHILI *, S.GAYATRI,

More information

Isocratic Reversed Phase Liquid Chromatographic Method Validation for the Determination of Cilostazol in Pure and Formulations

Isocratic Reversed Phase Liquid Chromatographic Method Validation for the Determination of Cilostazol in Pure and Formulations Human Journals Research Article October 2015 Vol.:4, Issue:3 All rights are reserved by Rambabu K et al. Isocratic Reversed Phase Liquid Chromatographic Method Validation for the Determination of Cilostazol

More information

Rao and Gowrisankar: Stability-indicating RP-HPLC Method for Pseudoephedrine, Ambroxol and Desloratadine

Rao and Gowrisankar: Stability-indicating RP-HPLC Method for Pseudoephedrine, Ambroxol and Desloratadine Research Paper Development and Validation of Stability-indicating RP- HPLC Method for the Estimation of Pseudoephedrine, Ambroxol and Desloratadine in Bulk and Tablet Dosage Forms N. MALLIKARJUNA RAO*

More information

DEVELOPMENT AND VALIDATION OF NEW HPLC METHOD FOR THE ESTIMATION OF PALIPERIDONE IN PHARMACEUTICAL DOSAGE FORMS

DEVELOPMENT AND VALIDATION OF NEW HPLC METHOD FOR THE ESTIMATION OF PALIPERIDONE IN PHARMACEUTICAL DOSAGE FORMS ISSN: 0974-1496 e-issn: 0976-0083 CODEN: RJCABP http://www.rasayanjournal.com http://www.rasayanjournal.co.in DEVELOPMENT AND VALIDATION OF NEW HPLC METHOD FOR THE ESTIMATION OF PALIPERIDONE IN PHARMACEUTICAL

More information

INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES

INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES Development of standardization of trikatu churna: A spectrophotometric approach Manjesh Thakur*, P. Porwal, P. Pal, K. Shukla and S.C. Mahajan Mahakal

More information

Development and Validation of Stability Indicating HPTLC Method for Estimation of Seratrodast

Development and Validation of Stability Indicating HPTLC Method for Estimation of Seratrodast ARC Journal of Pharmaceutical Sciences (AJPS) Volume 2, Issue 3, 2016, PP 15-20 ISSN 2455-1538 DOI: http://dx.doi.org/10.20431/2455-1538.0203004 www.arcjournals.org Development and Validation of Stability

More information

Research Article Simultaneous Estimation of DL-Methionine and Pyridoxine Hydrochloride in Tablet Dosage Form by RP-HPLC

Research Article Simultaneous Estimation of DL-Methionine and Pyridoxine Hydrochloride in Tablet Dosage Form by RP-HPLC Research Article Simultaneous Estimation of DL-Methionine and Pyridoxine Hydrochloride in Tablet Dosage Form by RP-HPLC Shinde Prashanti 1 *, Mane Aruna 2, Palled Mahesh 1, Bhat AR 1 and Karagane Swapna

More information

36 J App Pharm Vol. 6; Issue 1: 36-42; January, 2014 Rao et al., 2014

36 J App Pharm Vol. 6; Issue 1: 36-42; January, 2014 Rao et al., 2014 36 J App Pharm Vol. 6; Issue 1: 36-42; January, 2014 Rao et al., 2014 Original Research Article SIMPLE AND RAPID LIQUID CHROMATOGRAPHIC METHOD FOR REAL-TIME QUANTIFICATION OF NAPROXEN / ESOMEPRAZOLE MAGNESIUM

More information

ISSN: ; CODEN ECJHAO E-Journal of Chemistry 2011, 8(3),

ISSN: ; CODEN ECJHAO E-Journal of Chemistry  2011, 8(3), ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2011, 8(3), 1275-1279 Simultaneous Determination of Paracetamol, Phenylephrine Hydrochloride, Oxolamine Citrate and Chlorpheniramine

More information

Development And Validation Of HPTLC Method For Simultaneous Estimation Of Rutin And Quercetin In Hydroalcoholic Extract Of Triphala Churna

Development And Validation Of HPTLC Method For Simultaneous Estimation Of Rutin And Quercetin In Hydroalcoholic Extract Of Triphala Churna International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.4, No.4, pp 1457-1463, Oct-Dec 2012 Development And Validation Of HPTLC Method For Simultaneous Estimation Of Rutin

More information

Available online at Scholars Research Library

Available online at  Scholars Research Library Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2015, 7 (3):157-161 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4

More information

Development and validation of RP-HPLC method for simultaneous estimation of gliclazide and metformin in pure and tablet dosage form

Development and validation of RP-HPLC method for simultaneous estimation of gliclazide and metformin in pure and tablet dosage form ISSN: 2320-2831 IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: Research article Open Access Development and validation of RP-HPLC method for simultaneous estimation of gliclazide and metformin in

More information

A NOVEL RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF BERBERINE, QUERCETIN, AND PIPERINE IN AN AYURVEDIC FORMULATION

A NOVEL RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF BERBERINE, QUERCETIN, AND PIPERINE IN AN AYURVEDIC FORMULATION International Journal of Applied Pharmaceutics ISSN- 0975-7058 Vol 11, Issue 1, 2019 Original Article A NOVEL RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF BERBERINE, QUERCETIN, AND PIPERINE IN AN AYURVEDIC

More information

Journal of Chemical and Pharmaceutical Research

Journal of Chemical and Pharmaceutical Research Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(2):770-775 Validation of Rapid Liquid Chromatographic

More information

Pelagia Research Library

Pelagia Research Library Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2015, 6(1):6-10 ISSN: 0976-8688 CODEN (USA): PSHIBD Validated RP-HPLC method for simultaneous estimation of metformin hydrochloride

More information

Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:

Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: www.ajpamc.com VALIDATED RP-HPLC METHOD FOR DETERMINATION OF BROMHEXINE

More information

A simple validated RP-HPLC method for quantification of sumatriptan succinate in bulk and pharmaceutical dosage form

A simple validated RP-HPLC method for quantification of sumatriptan succinate in bulk and pharmaceutical dosage form IJPAR Vol.4 Issue 1 Jan-Mar-2015 Journal Home page: ISSN: 2320-2831 Research article Open Access A simple validated RP-HPLC method for quantification of sumatriptan succinate in bulk and pharmaceutical

More information

Dhull Rohit, Kumar Sanjay, Jalwal Pawan Department of Chemistry, OPJS Churu, Rajasthan, India

Dhull Rohit, Kumar Sanjay, Jalwal Pawan Department of Chemistry, OPJS Churu, Rajasthan, India International Journal of Advanced Science and Research ISSN: 2455-4227, Impact Factor: RJIF 5.12 www.allsciencejournal.com Volume 2; Issue 2; March 2017; Page No. 31-40 Validated gradient stability indicating

More information

International Journal of Medicine and Pharmaceutical Research. International Journal of Medicine and Pharmaceutical Research

International Journal of Medicine and Pharmaceutical Research. International Journal of Medicine and Pharmaceutical Research International Journal of Medicine and Pharmaceutical Research Journal Home Page: www.pharmaresearchlibrary.com/ijmpr Research Article Open Access Development and Validation of RP-HPLC Method for the Estimation

More information

Development and Validation of Stability Indicating HPLC method for Determination of Eperisone HCL in Bulk and in Formulation

Development and Validation of Stability Indicating HPLC method for Determination of Eperisone HCL in Bulk and in Formulation Development and Validation of Stability Indicating HPLC method for Determination of Eperisone HCL in Bulk and in Formulation Sanjay Patil 1, Suvarna Vanjari 2, Rajendra Patil 3, Tushar Deshmukh 4 1 TSSM

More information

Research Article Derivative Spectrophotometric Method for Estimation of Metformin Hydrochloride in Bulk Drug and Dosage Form

Research Article Derivative Spectrophotometric Method for Estimation of Metformin Hydrochloride in Bulk Drug and Dosage Form Research Article Derivative Spectrophotometric Method for Estimation of Metformin Hydrochloride in Bulk Drug and Dosage Form Gowekar NM, Lawande YS*, Jadhav DP, Hase RS and Savita N. Gowekar Department

More information

International Journal of Pharma and Bio Sciences

International Journal of Pharma and Bio Sciences International Journal of Pharma and Bio Sciences RESEARCH ARTICLE PHARMACEUTICAL ANALYSIS DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR ESTIMATION OF ESCITALOPRAM OXALATE IN TABLET DOSAGE

More information

Corresponding Author:

Corresponding Author: Adv J Pharm Life sci Res, 2017 5;3:1-8 ISSN 2454 3535 (On-line) RP-HPLC Method for Estimation of Mupirocin in Bulk and Pharmaceutical Formulation S.K.Attar 1 *, M.S.Kalshetti 2, N. A. Jadhao 3, N. R. Patel

More information

Ankit et al Journal of Drug Delivery & Therapeutics; 2013, 3(2), Available online at RESEARCH ARTICLE

Ankit et al Journal of Drug Delivery & Therapeutics; 2013, 3(2), Available online at  RESEARCH ARTICLE Ankit et al Journal of Drug Delivery & Therapeutics; 2013, 3(2), 26-30 26 Available online at http://jddtonline.info RESEARCH ARTICLE METHOD DEVELOPMENT AND ITS VALIDATION FOR QUANTITATIVE SIMULTANEOUS

More information

Estimation of Emtricitabine in Tablet Dosage Form by RP-HPLC

Estimation of Emtricitabine in Tablet Dosage Form by RP-HPLC Asian Journal of Chemistry Vol. 21, No. 8 (9), 5979-5983 Estimation of Emtricitabine in Tablet Dosage Form by RP-HPLC V. KIRAN KUMAR* and N. APPALA RAJU Department of Pharmaceutical Analysis, Sree Chaitanya

More information

Journal of Pharmacreations

Journal of Pharmacreations Journal of Pharmacreations ISSN: 2348-6295 Pharmacreations Vol.5 Issue 1 Jan- Mar- 2018 Journal Home page: www.pharmacreations.com Research article Open Access Method development and validation of apixaban

More information

SIMULTANEOUS ESTIMATION OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN TABLETS BY RP-HPLC METHOD

SIMULTANEOUS ESTIMATION OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN TABLETS BY RP-HPLC METHOD 170 Original Article SIMULTANEOUS ESTIMATION OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN TABLETS BY RP-HPLC METHOD *Lakshmana Rao A, 1 Bhaskara Raju V *V.V. Institute of Pharmaceutical Sciences, Gudlavalleru,

More information

A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY FOR LERCANIDIPINE HYDROCHLORIDE

A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY FOR LERCANIDIPINE HYDROCHLORIDE Int. J. Chem. Sci.: 6(1), 2008, 441-446 A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY FOR LERCANIDIPINE HYDROCHLORIDE S. APPALA RAJU, ARVIND B. KARADI and SHOBHA MANJUNATH HKES s College of Pharmacy,

More information

World Journal of Pharmaceutical Research

World Journal of Pharmaceutical Research World Journal of Pharmaceutical ReseaRch Volume 3, Issue 3, 4527-4535. Research Article ISSN 2277 715 DEVELOPMENT AND VALIDATION OF STABILITY INDICATING HPLC METHOD FOR ESTIMATION OF RAMOSETRON Zarana

More information

Airo International Research Journal ISSN : Volume : 7 October 2015

Airo International Research Journal ISSN : Volume : 7 October 2015 Airo International Research Journal ISSN : 2320-3714 Volume : 7 October 2015 METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF ESCITALOPRAM OXALATE AND ETIZOLAM IN TABLET DOSAGE FORM

More information

ANALYTICAL TECHNIQUES FOR THE QUALITY OF ANTI- AIDS DRUGS

ANALYTICAL TECHNIQUES FOR THE QUALITY OF ANTI- AIDS DRUGS Research Article N. Bala Krishna, IJPRBS, 2013; Volume 2(1): 63-77 ISSN: 2277-8713 IJPRBS N.BALAA KRISHNA, Dr. M.V.V NAGESWARA REDDY IJPRBS-QR CODE PAPER-QR CODE DEVELOPMENT OF NEW ANALYTICAL TECHNIQUES

More information

Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:

Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: www.ajpamc.com ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF GEFITINIB

More information

CHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW

CHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW 51 CHAPTER 2 SIMULTANEOUS ESTIMATION OF PIOGLITAZONE, GLIMEPIRIDE AND GLIMEPIRIDE IMPURITIES IN COMBINATION DRUG PRODUCT BY A VALIDATED STABILITY-INDICATING RP-HPLC METHOD 2.1 INTRODUCTION OF DOSAGE FORM

More information

A New Stability-Indicating and Validated RP-HPLC Method for the Estimation of Liraglutide in Bulk and Pharmaceutical Dosage Forms

A New Stability-Indicating and Validated RP-HPLC Method for the Estimation of Liraglutide in Bulk and Pharmaceutical Dosage Forms OPEN ACCESS Eurasian Journal of Analytical Chemistry ISSN: 1306-3057 2017 12(2):31-44 DOI 10.12973/ejac.2017.00152a A New Stability-Indicating and Validated RP-HPLC Method for the Estimation of Liraglutide

More information

RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF BACLOFEN IN BULK AND PHARMACEUTICAL DOSAGE FORMS

RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF BACLOFEN IN BULK AND PHARMACEUTICAL DOSAGE FORMS Int. J. Chem. Sci.: 11(1), 2013, 390-398 ISSN 0972-768X www.sadgurupublications.com RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF BACLOFEN IN BULK AND PHARMACEUTICAL DOSAGE FORMS SAROJ

More information

IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page:

IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: ISSN: 2320-2831 Research article Open Access Method development and validation of tenofovir disoproxil fumerate and emtricitabine in combined tablet

More information

Development and Validation of a Simultaneous HPLC Method for Quantification of Amlodipine Besylate and Metoprolol Tartrate in Tablets

Development and Validation of a Simultaneous HPLC Method for Quantification of Amlodipine Besylate and Metoprolol Tartrate in Tablets Journal of PharmaSciTech 0; ():- Research Article Development and Validation of a Simultaneous HPLC Method for Quantification of Amlodipine Besylate and Metoprolol Tartrate in Tablets * Sayyed Hussain,

More information

SULTANA SHAIKH, VANDANA JAIN *

SULTANA SHAIKH, VANDANA JAIN * International Journal of Applied Pharmaceutics ISSN- 0975-7058 Vol 10, Issue 3, 2018 Original Article DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF QUERCETIN, ELLAGIC

More information

Pharmacological Study of Symplocos racemosa Roxb

Pharmacological Study of Symplocos racemosa Roxb Human Journals Research Article April 2017 Vol.:9, Issue:1 All rights are reserved by Srabana Maitra (PAUL) et al. Pharmacological Study of Symplocos racemosa Roxb Keywords: Symplocos racemosa, antioxidant,

More information

RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR CILOSTAZOL IN TABLET DOSAGE FORM

RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR CILOSTAZOL IN TABLET DOSAGE FORM IJPAR Vol.5 Issue 4 Oct - Dec -2016 Journal Home page: ISSN:2320-2831 Research article Open Access RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR CILOSTAZOL IN TABLET DOSAGE FORM *1 M. Swapna *, 2 M.Vijay

More information

Development and Validation of Analytical Method for Determination of Andrographolide in Bulk Powder

Development and Validation of Analytical Method for Determination of Andrographolide in Bulk Powder DOI:10.21276/ijprhs.2019.01.08 Y Pancham et al. CODEN (USA)-IJPRUR, e-issn: 2348-6465 International Journal of Pharma Research and Health Sciences Available online at www.pharmahealthsciences.net Original

More information

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR DETERMINATION OF ROSUVASTATIN CALCIUM IN BULK AND PHARMACEUTICAL DOSAGE FORM

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR DETERMINATION OF ROSUVASTATIN CALCIUM IN BULK AND PHARMACEUTICAL DOSAGE FORM Research Article DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR DETERMINATION OF ROSUVASTATIN CALCIUM IN BULK AND PHARMACEUTICAL DOSAGE FORM Chirag B. Pandya*, K.P. Channabasavaraj, Jaydeep D. Chudasama,

More information

Asian Journal of Chemistry Vol. 21, No. 1 (2009),

Asian Journal of Chemistry Vol. 21, No. 1 (2009), Asian Journal of Chemistry Vol. 21, o. 1 (2009), 176-182 imultaneous Estimation of Related Impurities of Tizanidine Hydrochloride in its Active Pharmaceutical Ingradient by Reversed-Phase Liquid Chromatography

More information

Development and validation of a RP-HPLC method for the simultaneous determination of Embelin, Rottlerin and Ellagic acid in Vidangadi churna

Development and validation of a RP-HPLC method for the simultaneous determination of Embelin, Rottlerin and Ellagic acid in Vidangadi churna Journal of Pharmaceutical Analysis 2012;2(5):366 371 Contents lists available at ScienceDirect Journal of Pharmaceutical Analysis www.elsevier.com/locate/jpa www.sciencedirect.com SHORT COMMUNICATION Development

More information

REVERSE PHASE HPLC METHOD FOR THE ANALYSIS OF ALFUZOSIN HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORMS

REVERSE PHASE HPLC METHOD FOR THE ANALYSIS OF ALFUZOSIN HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORMS Int. J. Chem. Sci.: 6(1), 2008, 399-404 REVERSE PHASE HPLC METHOD FOR THE ANALYSIS OF ALFUZOSIN HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORMS S. APPALA RAJU, ARVIND B. KARADI and SHOBHA MANJUNATH HKES s

More information

10 Kadam V. N. et al. Ijppr.Human, 2014; Vol. 1(2): 10-21 (Research Article) DEVELOPMENT AND VALIDATION OF ANALYTICAL METHODS FOR SIMULTANEOUS ESTIMATION OF VOGLIBOSE, GLIMEPIRIDE AND METFORMIN HYDROCHLORIDE

More information

Comparison of conventional HPLC with UPLC method for determination of albuterol sulfate and it s impurities in pharmaceutical formulation

Comparison of conventional HPLC with UPLC method for determination of albuterol sulfate and it s impurities in pharmaceutical formulation Oriental Journal of Chemistry Vol. 24(2), 537-544 (2008) Comparison of conventional HPLC with UPLC method for determination of albuterol sulfate and it s impurities in pharmaceutical formulation P.N. DALVI,

More information

SIMULTANEOUS DETERMINATION OF ATORVASTATIN AND EZETIMIBE BY RP-HPLC IN PURE AND PHARMACEUTICAL DOSAGE FORM

SIMULTANEOUS DETERMINATION OF ATORVASTATIN AND EZETIMIBE BY RP-HPLC IN PURE AND PHARMACEUTICAL DOSAGE FORM Int. J. Chem. Sci.: 6(3), 2008, 1576-1582 SIMULTANEOUS DETERMINATION OF ATORVASTATIN AND EZETIMIBE BY RP-HPLC IN PURE AND PHARMACEUTICAL DOSAGE FORM B. NEELIMA, P. RAVI KUMAR, M. MURALI KRISHNA, V. HIMA

More information

SIMULTANEOUS QUANTIFICATION OF TELMISARTAN AND METOPROLOL SUCCINATE IN TABLETS BY LIQUID CHROMATOGRAPHY

SIMULTANEOUS QUANTIFICATION OF TELMISARTAN AND METOPROLOL SUCCINATE IN TABLETS BY LIQUID CHROMATOGRAPHY World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.com/ Short

More information

Scholars Research Library

Scholars Research Library Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2010, 2(2): 294-299 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4

More information

Hyderabad, India. Department of Pharmaceutical Chemistry, Glocal University, Saharanpur, India.

Hyderabad, India. Department of Pharmaceutical Chemistry, Glocal University, Saharanpur, India. International Journal On Engineering Technology and Sciences IJETS RP-HPLC Method development and validation for the Simultaneous Estimation of Metformin and Empagliflozine in Tablet Dosage Form Shaik

More information

Vol-3, Issue-4, Suppl-1, Nov 2012 ISSN: Ghodasara et al PHARMA SCIENCE MONITOR

Vol-3, Issue-4, Suppl-1, Nov 2012 ISSN: Ghodasara et al PHARMA SCIENCE MONITOR PHARMA SCIENCE MONITOR AN INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES HPTLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF DIOSGENIN AND GALLIC ACID IN MARKETED FORMULATION Ghodasara

More information

Development of a Validated RP-HPLC Method for the Analysis of Citicoline Sodium in Pharmaceutical Dosage Form using Internal Standard Method

Development of a Validated RP-HPLC Method for the Analysis of Citicoline Sodium in Pharmaceutical Dosage Form using Internal Standard Method Research Article Development of a Validated RP-HPLC Method for the Analysis of Citicoline Sodium in Pharmaceutical Dosage Form using Internal Standard Method * S. M. Sandhya, G. Jyothisree, G. Babu Department

More information

Pankti M. Shah et al, Asian Journal of Pharmaceutical Technology & Innovation, 04 (17); 2016; 07-16

Pankti M. Shah et al, Asian Journal of Pharmaceutical Technology & Innovation, 04 (17); 2016; 07-16 Asian Journal of Pharmaceutical Technology & Innovation ISSN: 2347-8810 Research Article Received on: 30-03-2016 Accepted on: 01-04-2016 Published on: 15-04-2016 Corresponding Author: *Pankti M. Shah,

More information

Development and Validation for Simultaneous Estimation of Sitagliptin and Metformin in Pharmaceutical Dosage Form using RP-HPLC Method

Development and Validation for Simultaneous Estimation of Sitagliptin and Metformin in Pharmaceutical Dosage Form using RP-HPLC Method International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.5, No.4, pp 1736-1744, Oct-Dec 2013 Development and Validation for Simultaneous Estimation of Sitagliptin and Metformin

More information

Preliminary Phytochemical Screening and HPTLC Fingerprinting of Bark Extracts of Symplocos racemosa

Preliminary Phytochemical Screening and HPTLC Fingerprinting of Bark Extracts of Symplocos racemosa 2013; 2 (3): 4549 ISSN 22784136 ISSN 23498234 JPP 2013; 2 (3): 4549 2013 AkiNik Publications Received: 1872013 Accepted: 0982013 Preliminary Phytochemical Screening and HPTLC Fingerprinting of Bark s of

More information

Key Words: Halobetasol, Fusidic acid, RP-HPLC.

Key Words: Halobetasol, Fusidic acid, RP-HPLC. Asian Journal of Chemistry Vol. 22, No. 5 (2010), 3376-3380 Simultaneous Determination of alobetasol Propionate and Fusidic Acid Related Substances by Reversed Phase igh Performance Liquid Chromatographic

More information

International Journal of Research in Pharmaceutical and Nano Sciences Journal homepage:

International Journal of Research in Pharmaceutical and Nano Sciences Journal homepage: Research Article CODEX: IJRPJK ISSN: 2319 9563 International Journal of Research in Pharmaceutical and Nano Sciences Journal homepage: www.ijrpns.com HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS

More information

AMERICAN JOURNAL OF BIOLOGICAL AND PHARMACEUTICAL RESEARCH

AMERICAN JOURNAL OF BIOLOGICAL AND PHARMACEUTICAL RESEARCH AMERICAN JOURNAL OF BIOLOGICAL AND PHARMACEUTICAL RESEARCH e-issn - 2348-2184 Print ISSN - 2348-2176 Journal homepage: www.mcmed.us/journal/ajbpr VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF

More information

Method Development and Validation for Simultaneous Estimation of Atorvastatin and Ezetimibe in Pharmaceutical Dosage Form by HPLC

Method Development and Validation for Simultaneous Estimation of Atorvastatin and Ezetimibe in Pharmaceutical Dosage Form by HPLC World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.org/ Original

More information

Journal of Chemical and Pharmaceutical Research, 2017, 9(9): Research Article

Journal of Chemical and Pharmaceutical Research, 2017, 9(9): Research Article Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2017, 9(9):70-80 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development and Validation of Stability Indicating

More information

Development and Validation of RP-HPLC Method for the Estimation of Gemigliptin

Development and Validation of RP-HPLC Method for the Estimation of Gemigliptin Human Journals Research Article September 2018 Vol.:13, Issue:2 All rights are reserved by Hajera Khan et al. Development and Validation of RP-HPLC Method for the Estimation of Gemigliptin Keywords: Gemigliptin,

More information

A stability indicating RP-HPLC method for simultaneous estimation of darunavir and cobicistat in bulk and tablet dosage form

A stability indicating RP-HPLC method for simultaneous estimation of darunavir and cobicistat in bulk and tablet dosage form Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (12):89-96 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4

More information

METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR DETERMINATION OF NEW ANTIDIABETIC AGENT LINAGLIPTIN IN BULK AND IN PHARMACEUTICAL FORMULATION

METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR DETERMINATION OF NEW ANTIDIABETIC AGENT LINAGLIPTIN IN BULK AND IN PHARMACEUTICAL FORMULATION International Journal of Medicinal Chemistry & Analysis METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR DETERMINATION OF NEW ANTIDIABETIC AGENT LINAGLIPTIN IN BULK AND IN PHARMACEUTICAL FORMULATION

More information

A Validated Chiral Liquid Chromatographic Method for The Enantiomeric Separation of Dapoxetine Hydrochloride

A Validated Chiral Liquid Chromatographic Method for The Enantiomeric Separation of Dapoxetine Hydrochloride Received on 15/05/2012; Revised on 22/05/2012; Accepted on 09/06/2012 A Validated Chiral Liquid Chromatographic thod for The Enantiomeric Separation of Dapoxetine Hydrochloride T.Rohith 1 and S. Ananda

More information

Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Haloperidol and Trihexyphenidyl in API and Combined Tablet Dosage Form

Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Haloperidol and Trihexyphenidyl in API and Combined Tablet Dosage Form Amulya ORIGINAL E et al.: Development RESEARCH and Validation of RP-HPLC Method ISSN: 2455-8095 doi: https://doi.org/10.21477/ijapsr.3.3.3 Development and Validation of RP-HPLC Method for the Simultaneous

More information

VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DEXAMETHASONE AND GRANISETRON IN COMBINED DOSAGE FORMS

VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DEXAMETHASONE AND GRANISETRON IN COMBINED DOSAGE FORMS INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DEXAMETHASONE AND GRANISETRON IN COMBINED

More information

MEDAK DIST. ANDHRA PRADESH STATE, INDIA. Research Article RECEIVED ON ACCEPTED ON

MEDAK DIST. ANDHRA PRADESH STATE, INDIA. Research Article RECEIVED ON ACCEPTED ON Page67 Available Online through IJPBS Volume 1 Issue 2 APRIL- JUNE 2011 SIMPLE QUANTITATIVE METHOD DEVELOPMENT AND VALIDATION OF VALSARTAN IN PUREFORM AND PHARMACEUTICAL DOSAGE FORMS BYUV SPECTROSCOPY

More information

New RP-HPLC Method for the Determination of Fludarabine in Pharmaceutical Dosage Forms

New RP-HPLC Method for the Determination of Fludarabine in Pharmaceutical Dosage Forms Online : ISSN 2349-669X Print : ISSN 0973-9874 New RP-HPLC Method for the Determination of Fludarabine in Pharmaceutical Dosage Forms LAKSHMI B 1, *, RAMA KRISHNA K 2 AND JAYAVEERA K N 3 1. Department

More information

Research Article. ISSN Available online at 746

Research Article. ISSN Available online at   746 ISSN 2395-3411 Available online at www.ijpacr.com 746 Research Article Development and Validation of New Analytical Methods for Simultaneous Estimation of Ramipril and Metoprolol succinate by HPLC method

More information