PHYTOCHEMICAL SCREENING AND ANTI MICROBIAL STUDIES OF HEMIDESMUS INDICUS
|
|
- Evangeline Thornton
- 5 years ago
- Views:
Transcription
1 Volume-6, Issue-4, Oct-Dec-2015 Coden IJABFP-CAS-USA Received: 12 th July-2015 Revised: 5 th Aug Accepted: 7 th Aug-2015 Research article PHYTOCHEMICAL SCREENING AND ANTI MICROBIAL STUDIES OF HEMIDESMUS INDICUS Jayasimha Rayalu Daddam 1*, Muralidhara Rao 2 and D.S.Rao 3 1 Department of Biotechnology, JNTUA, Anantapuram, Andhra Pradesh. INDIA 2 Department of Biotechnology, Sri Krishnadevaraya University, Anantapuram, Andhra Pradesh. INDIA 3 Department of Chemical Engineering, JNTUA, Anantapuram, Andhra Pradesh. INDIA. *Corresponding Author Address: jayasimharayalu@gmail.com Phone: ABSTRACT: Many forms of alternative medicines were available for those who cannot be helped by conventional medicine. Ayurvedha and Herbal medicine were two important forms of alternative medicine that was widely available in India. This work was mainly concerned with the identification of the therapeutic properties of Hemidesmus indicus. The ethanolic extract of hemidesmus indicus root was used for its anti oxidant and antimicrobial activity. Hemidesmus indicus root extract has very well anti oxidant and anti microbial activity. The ethanolic extract of Hemidesmus indicus was checked for anti microbial activity against pathogenic bacteria such as staphylococcus aures, pseudomonas aeruginosa and fungi Aspergillus niger. Key words: Hemidesmus indicus, Antioxidant activity, anti microbial activity, phytochemical screening. INTRODUCTION Hemidesmus indicus commonly brown as Indian Sarsaparilla, belonging to the family Asclepiadaceae, is a slender laticiferous, twining, sometimes prostrate or semi erect shrub, occurring over the greater part of India. Roots are woody and aromatic; stems numerous, slender, terete, thickened at the nodes; leaves opposite, short-petioled, very variable, elliptic-oblong to linear-lanceolate often variegated with white above, sometimes silvery white and pubescent beneath, flowers are greenish outside, purplish inside, crowded in subsessile aeillary cymes; follicles are slender, four inches long, cylindrical, sometimes curved, divaricate; seeds numerous, black, flattened, with a silvery white coma. This is a common medicinal plant widely used in Indian Systems of Medicine (Anonymous, 1997) and also an official drug in Indian Pharmacopoeia (Anonymous, 1996) and British Pharmacopoeia (Anonymous, 2003). Various market samples are available in the name, identified as H. indicus, Decalepis hamiltoni and Cryptokpis buchanani belonging to the family Asclepiadaceae; lchnocarpta frutescens and Vallaris solanaceae of the family Apocyanaceae. Apart from this H. indicus exists with two variants namely var. indicus and var. pubescens, which are not given much emphasis. Hence the review was carried out to enumerate the benefits of H. indicus (Anonymous (1986, 1997), Mukherjee, 1980). The roots are used as antipyretic, anti-diarrhoeal, astringent, blood purifier, diaphoretic, diuretic, refrigerant and tonic (Anonymous, 1986, 1997; Nadkarni, 1989). Roots are useful in biliousness, blood diseases, dysentery, diarrhoea, respiratory disorders, skin diseases, syphilis, fever, leprosy, leucoderma, leucorrhoea, itching, bronchitis, asthma, eye diseases, epileptic fits in children, kidney and urinary disorders, loss of appetite, burning sensation and rheumatism (Mukherjee, 1953; Chandra et al 1995; Anonymous, 1986; Nadkarni, 1989). Root bark is used to cure dyspepsia, loss of appetite, nutritional disorders, fever, skin diseases, ulcer, syphilis, rheumatism and leucorrhoea (Nadkarni, 1989). Stem of H. indicus is used as diaphoretic, diuretic, laxative and in treating brain, liver and kidney diseases, syphilis, sleet, urinary discharges, uterine complaints, leucodenna, cough and asthma (Bhandary et al 1995). International Journal of Applied Biology and Pharmaceutical Technology Page: 10
2 MATERIAL AND METHODS Collection of Plant Materials The fresh and healthy roots of Hemidesmus indicus were collected. The plant specimens were identified in Department of Biotechnology, Sri krishnadevaraya University, Anantapuram. Preparation of Root Extract of Hemidesmus indicus The extraction of roots of Hemidesmus indicus was carried out using known standard procedures. The roots were dried in shade and powdered in a mechanical grinder. The powder (10.0 g) was initially defatted with ethyl alcohol by using a Soxhlet extractor for 72 hours at a temperature not exceeding the boiling point of the solvent. The extracts were filtered using Whatman filter paper (No.1) while hot, concentrated in vacuum under reduced pressure using rotary flask evaporator, and dried in a desiccator. The ethyl alcoholic root extract yields a dark reddish residue weighing 4.50 g (45.0% w/w). This crude extracts of ethylalcohol was used for further investigation for potential of antimicrobial properties. Preliminary Phytochemical Screening The root extracts were subjected to preliminary phytochemical testing to detect for the presence of different chemical groups of compounds. Air-dried and root powder was screened for the presence of saponins, tannins, alkaloids, flavonoids, triterpenoids, stroids, glycosides, anthraquinones, coumarin, saponins, gum, mucilage, carbohydrates, reducing sugars, starch, protein, and amino acids. Test Microorganisms and Growth Media Staphylococcus aureus (MTCC 3160), Pseudomonas aeruginosa (MTCC 1688) and fungal strain Aspergillus niger (MTCC 1785) were chosen based on their clinical and pharmacological importance. The bacterial strains obtained from Department of Microbiology, Osmania University, were used for evaluating antimicrobial activity. The bacterial and fungal stock cultures were incubated for 24 hours at 37 C on nutrient agar and potato dextrose agar (PDA) medium, respectively, following refrigeration storage at 4 C. The bacterial strains were grown in Mueller- Hinton agar (MHA) plates at 37 C (the bacteria were grown in the nutrient broth at 37 C and maintained on nutrient agar slants at 4 C), whereas the fungi were grown in Sabouraud dextrose agar and PDA media, respectively, at 28 C. The stock cultures were maintained at 4 C. Antimicrobial Activity Whatman No: 1 filter paper discs of 6mm diameter are prepared and autoclaved by keeping in a clean and dry Petri plate. The filter paper discs were soaked in plant extracts for 6 hours are taken as test material. After 6 hours the discs were shade dried. The concentrations of root extracts per disc are accounted for 0.1 grams/1ml. Subsequently they are carefully transferred to spread on cultured Petri plates. Filter paper discs immersed in ethanol, benzene, distilled water are prepared and used as control. Testing of antimicrobial activity: To test the antimicrobial activity on agar plates, LB agar medium was prepared using the ingredients mentioned above. The medium was sterilized at 121 C for 30 min s. The agar test plates were prepared by pouring about 15ml of the medium into 10cm Petri dishes under aseptic condition and left undisturbed for 2hrs to solidify the medium. 1ml of inocculum (containing suspension) of P.aeruginosa and Sta.aureus was poured to the respective plates separately containing solidified agar media. Six replicates were maintained. The prepared sterile whatman no :1 filter paper discs of 6mm diameter were impregnated with the extracts and shaken thoroughly and this test plates incubated for a period of 48 hrs in BOD at 37ºC for the development of inhibitory zones and the average of 2 independent readings for each organism in different extracts were recorded. The control Petri plates and also maintained above respective cultures Measuring the diameter of inhibition zone: The inhibition zones were lead after 1 day at 37ºC for bacteria. The diameter of the inhibition zone was measured and recorded with the aid of plastic ruler. 7 paper discs placed in 1 Petri plate. RESULTS AND DISCUSSION It was found that ethyl alcoholic extracts of Hemidesmus indicus roots contained tannins, flavonoids, saponins, triterpenoids, steroids, glycosides, anthraquinones, reducing sugars, carbohydrates, proteins, and amino acids. Antimicrobial activity Ethanolic extract of Hemidesmus indicus root International Journal of Applied Biology and Pharmaceutical Technology Page: 11
3 Fig 1: Pseudomonas aeruginosa Fig 2: Staphylococcus aureus Fig 3: Aspergillus niger Table 1: Inhibitory activities of Root extract of Hemidesmus indicus on microorganisms Zone of inhibition (MIC) Pseudomonas Plants Staphylococcus aureus Aspergillus aeruginosa (+ve) (mm) niger (mm) (-ve) (mm) Hemidesmus indicus PHYTOCHEMICAL SCREENING OF PLANT LEAVES IN DIFFERENT EXTRACTS Table 2: Hemidesmus indicus phyto chemical Screning S.No Secondary metabolites Hexane Ethyl acetate Ethanolic Aqueous 1 Steroids Triterpenes Saponins Tri terpinoidal saponins Alkaloids Carbohydrates Flavonoids Tannins Glycosides Polyphenols International Journal of Applied Biology and Pharmaceutical Technology Page: 12
4 CONCLUSION In the present study it was found that Hemidesmus indicus root extract has an excellent antimicrobial activity. The pathogenic bacteria like Pseudomonas aeruginosa, Staphylococcus aureus and fungus Aspergillus niger were inhibited in presence of the root extracts of Hemidesmus indicus ethanolic extract. Therefore the future studies should be aimed to exploit this plant to be used as one of the best medicinal plant is controlling pathogenic bacteria. REFFERENCES Anonymous. (1986). The useful plants of India. CSIR, New Delhi, India. Anonymous, (1996). Indian Pharmacopoeia. Indian Pharmacopoeia Committee, Ministry of Health and Family Welfare, Government of India, New Delhi. Anonymous. (1997). The Wealth of India. Raw materials, Vol. III, V and X, CSIR, New Delhi, India. Anonymous. (2003). British Pharmacopoeia. British Pharmacopoeia Commission, Market Towers, Nine Elms Lane, London. Bhandary, M.J.M. Chandrashekar K.R. and Icaveriappe K.M. (1995). Medical ethnobotany of the siddis of Uttara Kannada district, Karnataka. India J. Ethnophannacol., 47: Mukherjee, B. (1953). The Indian pharmaceutical codex, Vol. II, Indigenous drugs. CSIR, New Delhi, India. Mukherjee, K. and L.N. Ray. (1980). Screening of some Indian plant species. Q. J. Crude Drug Res., 18: Nadkami, A.N. (1989). Indian Material Media Vol. I, Popular Book Depot, Bombay, India. International Journal of Applied Biology and Pharmaceutical Technology Page: 13
5
STUDIES ON TEPHROSIA PURPUREA: A PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL STUDIES
Volume-5, Issue-4, Oct-Dec-2015 Coden: IJPAJX-CAS-USA, Copyrights@2015 ISSN-2231-4490 Received: 12 th Aug-2015 Revised: 29 th Aug-2015 Accepted: 30 th Aug-2015 Research article STUDIES ON TEPHROSIA PURPUREA:
More informationHigher plants produced hundreds to thousands of diverse chemical compounds with different biological activities (Hamburger and Hostettmann, 1991).
4. ANTIMICROBIAL ACTIVITY OF PHYSALIS MINIMA L. 4.1. Introduction Use of herbal medicines in Asia represents a long history of human interactions with the environment. Plants used for traditional medicine
More informationIn vitro antimicrobial activity of leaves and bark extracts of Ficus religiosa (Linn.)
INDIAN JOURNAL OF PHARMACEUTICAL & BIOLOGICAL RESEARCH (IJPBR). Vol. 1 (1), March., 13 In vitro antimicrobial activity of leaves and bark extracts of Ficus religiosa (Linn.) G.Ramakrishnaiah * 1, T. Hariprasad
More informationPHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITIES OF A MEDICINAL PLANT STERCULIA VILLOSA. Ch.Subba Lakshmi 1* and T.Pullaiah 2
Volume-5, Issue-1, Jan-Mar-2015 Coden: IJPAJX-USA, Copyrights@2015 ISSN-2231-4490 Received: 20 th Oct-2014 Revised: 19 th Nov-2014 Accepted: 22 nd Nov-2014 Research article PHYTOCHEMICAL SCREENING AND
More informationInternational Journal of Scientific & Engineering Research, Volume 7, Issue 8, August ISSN
International Journal of Scientific & Engineering Research, Volume 7, Issue 8, August-2016 105 Antimicrobial activity of Andrographis paniculata stem extracts. S.Gurupriya 1 and Dr.L.Cathrine 2 1 M.phil
More informationIn vitro study of antibacterial activity of Carissa carandas leaf extracts
Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 2012, 2 (1):36-40 ISSN : 2249-7412 CODEN (USA): AJPSKY In vitro study of antibacterial activity of Carissa
More informationOcta Journal of Biosciences
Octa Journal of Biosciences ISSN 2321 3663 International peer-reviewed journal June 2014 Octa. J. Biosci. Vol. 2(1):59-63 Octa Journal of Biosciences journal homepage: www.sciencebeingjournal.com Antimicrobial
More informationBioprospecting of Neem for Antimicrobial Activity against Soil Microbes
ISSN: 2454-132X Impact factor: 4.295 (Volume3, Issue1) Available online at: www.ijariit.com Bioprospecting of Neem for Antimicrobial Activity against Soil Microbes R. Prasanna PRIST University, Tamilnadu
More informationPhytochemical screening and antibacterial properties of Garcinia kola
2013; 2(3): 34-38 Online at: www.phytopharmajournal.com Research Article ISSN 2230-480X JPHYTO 2013; 2(3): 34-38 2013, All rights reserved Phytochemical screening and antibacterial properties of Garcinia
More informationINTERNATIONAL JOURNAL OF INSTITUTIONAL PHARMACY AND LIFE SCIENCES
International Standard Serial Number (ISSN): 2249-6807 International Journal of Institutional Pharmacy and Life Sciences 5(4): July-August 2015 INTERNATIONAL JOURNAL OF INSTITUTIONAL PHARMACY AND LIFE
More informationAsian Journal of Phytomedicine and Clinical Research Journal home page:
Research Article CODEN: AJPCFF ISSN: 2321-0915 Asian Journal of Phytomedicine and Clinical Research Journal home page: www.ajpcrjournal.com PHARMACOGNOSTIC, PHYTOCHEMICAL AND BIOLOGICAL STUDIES OF LEAVES
More informationPuducherry. Antimicrobial activity, Crude drug extraction, Zone of Inhibition, Culture Media, RVSPHF567.
ANTI-MICROBIAL ACTIVITY OF THE CRUDE DRUGS AND THE POLYHERBAL FORMULATION (RVSPHF567) BY STANDARDIZED CUP AND PLATE METHOD C.S. Kandasamy 1,2*, Suman Nath 2, P. Arulraj 1,2, V. Gopal 3, P. Muthusamy 4,
More informationChandan Prasad.et.al. Int. Journal of Engineering Research and Application ISSN : , Vol. 7, Issue 9, ( Part -6) September 2017, pp.
RESEARCH ARTICLE OPEN ACCESS Antibacterial properties of Unripened Fruits of Trapa natans, Annona squamosa, Ficus carica and Anthocephalus cadamba against Pathogenic Gram Positive and Negative Bacteria.
More informationAntimicrobial Potential of Whole Plant and Callus Extract of Aristolochia bracteolata Lam
Human Journals Research Article October 2016 Vol.:7, Issue:3 All rights are reserved by A. Lavanya et al. Antimicrobial Potential of Whole Plant and Callus Extract of Aristolochia bracteolata Lam Keywords:
More informationANTIMICROBIAL AND PHYTOCHEMICAL SCREENING OF TRAGIA INVOLUCRATA L. USING UV-VIS AND FTIR
ANTIMICROBIAL AND PHYTOCHEMICAL SCREENING OF TRAGIA INVOLUCRATA L. USING UV-VIS AND FTIR Sahaya Sathish S, Vijayakanth P, Palani R, Thamizharasi T and Vimala A Department of Botany, St. Joseph s College
More informationCHAPTER 8 ANTIBACTERIAL ACTIVITY OF THE CRUDE ETHANOLIC EXTRACT AND THE ISOLATED COMPOUNDS FROM THE STEM OF COSTUS IGNEUS
CHAPTER 8 ANTIBACTERIAL ACTIVITY OF THE CRUDE ETHANOLIC EXTRACT AND THE ISOLATED COMPOUNDS FROM THE STEM OF COSTUS IGNEUS 8.1 INTRODUCTION Medicinal plants are the backbone of traditional medicine and
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2010, 2(6):424-428 Antibacterial activity of Moringa Oleifera
More informationANTIBACTERIAL EFFECTS OF CRUDE EXTRACT OF Azadirachta indica AGAINST Escherichia coli and Staphylococcus aureus
International Journal of Science, Environment and Technology, Vol. 2, No 5, 2013, 989 993 ISSN 2278-3687 (O) ANTIBACTERIAL EFFECTS OF CRUDE EXTRACT OF Azadirachta indica AGAINST Escherichia coli and Staphylococcus
More informationPharmacologyonline 2: (2011) ewsletter Tiwari et al. A EVALUATIO OF A TIMICROBIAL ACTIVITIES OF ROOT EXTRACT OF CALE DULA OFFICI ALIS (LI.
A EVALUATIO OF A TIMICROBIAL ACTIVITIES OF ROOT EXTRACT OF CALE DULA OFFICI ALIS (LI.) *Prashant Tiwari 1, Ritesh Jain 1, Kuldeep Kumar 2, Rajnikant Panik 3, Pratap Kumar Sahu 4 1. School of Pharmacy,
More informationPHYTOCHEMICAL SCREENING AND ANTICANDID ACTIVITY OF SELECTED PLANTS OF GENUS CAESALPINIA
PHYTOCHEMICAL SCREENING AND ANTICANDID ACTIVITY OF SELECTED PLANTS OF GENUS CAESALPINIA A. Radhaiah, B. Suman, B. Suresh Babu* and D. Muniswamy Department of Botany, S.V.A. Govt. Degree College (M), Srikalahasti,
More informationEVALUATION OF ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF Zingiber officinale (GINGER) RHIZOME EXTRACT
International Journal of Allied Practice, Research and Review Website: www.ijaprr.com (ISSN 2350-1294) EVALUATION OF ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF Zingiber officinale (GINGER) RHIZOME
More informationInternational Journal of Research in Pharmaceutical and Nano Sciences Journal homepage:
Research Article CODEN: IJRPJK ISSN: 2319 9563 International Journal of Research in Pharmaceutical and Nano Sciences Journal homepage: www.ijrpns.com SYNERGISTIC ANTIBACTERIAL ACTIVITY OF CLOVE, CUMIN
More informationEvaluation of antimicrobial activity and Bidens biternata ehrenb Leaves
Human Journals Research Article October 2015 Vol.:1, Issue:4 All rights are reserved by Nadiminti Satish Reddy et al. Evaluation of antimicrobial activity and Bidens biternata ehrenb Leaves Keywords: Bidens
More informationAntibacterial properties of Anthocephalus cadamba fruits
Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 2011, 1 (2):1-7 ISSN : 2249 7412 Antibacterial properties of Anthocephalus cadamba fruits * Ram Prakash Mishra
More informationA study on phytochemical analysis and antimicrobial activity of Hyptis suaveolens (L.) poit
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(6):438-442 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 A study on phytochemical analysis and antimicrobial
More informationAntimicrobial Activity of Black Fruit Variant of Solanum nigrum L.
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 6 (2017) pp. 2706-2713 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.605.303
More informationScreening of Antibacterial Activity of Aqueous Bark Extract of Bombax ceiba against some Gram Positive and Gram Negative Bacteria
Screening of Antibacterial Activity of Aqueous Bark Extract of Bombax ceiba against some Gram Positive and Gram Negative Bacteria Original Article Digge V.G 1, Kuthar Sonali S 2, Hogade Maheshwar G 3,
More informationPRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF DIFFERENT EXTRACTS OF Saccharum officinarum Linn (Ikshu) ROOTS
International Journal of Science, Environment and Technology, Vol. 5, No 4, 2016, 2315 2322 ISSN 2278-3687 (O) 2277-663X (P) PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF DIFFERENT
More informationWORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES. World Journal of Pharmacy and Pharmaceutical Sciences SJIF Impact Factor 6.
WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES Neeru et al. SJIF Impact Factor 6.041 Volume 5, Issue 6, 1884-1891 Research Article ISSN 2278 4357 DETERMINATION OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID
More informationInternational Journal of Pharma and Bio Sciences
Research Article Microbiology International Journal of Pharma and Bio Sciences ISSN 0975-6299 PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF CLITORIA TERNATEA LEAVES AGAINST STAPHYLOCOCCUS AUREUS
More informationAvailable online at
Available online at www.ijpab.com ISSN: 2320 7051 Int. J. Pure App. Biosci. 3 (1): 203-206 (2015) INTERNATIONAL JOURNAL OF PURE & APPLIED BIOSCIENCE Research Article Antifungal Activity of the Plant Extracts
More informationResearch Journal of Pharmaceutical, Biological and Chemical Sciences
ISSN: 09758585 Research Journal of Pharmaceutical, Biological and Chemical Sciences Detection of Antimicrobial Activity of Oscimum sanctum (Tulsi) & Trigonella foenum graecum (Methi) against some selected
More informationAvailable online at Phytochemical analysis and inhibitory activity of Ornamental Plant (Bougainvillea spectabilis)
Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 13, 3(2):1- ISSN : 2249-7412 CODEN (USA): AJPSKY Phytochemical analysis and inhibitory activity of Ornamental
More informationIn vitro Evaluation of Antibacterial Activity of Bark and Flower Extracts of Pimenta officinalis Lindl.
ADVANCES IN BIORESEARCH, Vol 1 [2] December 2010: 61-68 Society of Education, India http://www.soeagra.com ISSN 0976-4585 RESEARCH PAPER In vitro Evaluation of Antibacterial Activity of Bark and Flower
More informationAntibacterial Activity of Leaves of BAMBOO
Vijay kumar Singh 1 *, Rahul Shukla 1, Satish V 2, Shankul Kumar 2,Sumit Gupta 3 and Ashutosh Mishra 4 1 Dept. Of & Phytochemistry, KNIMT, SULTANPUR, U.P. 2 G.H.B. college of Pharmacy, Aniyad, Gujrat.
More informationJigna Parekh, Nehal Karathia and Sumitra Chanda*
African Journal of Biomedical Research, Vol. 9 (); 53-5 ISSN 1119 59 Ibadan Biomedical Communications Group Full Length Research Article Evaluation of antibacterial activity and phytochemical analysis
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2010, 2(6):435-440 Antibacterial activity of Plumeria rubra Linn.
More informationComparism of the Antimicrobial Activities of N-hexane and Diethyl Ether Extracts of Garcinia kola Heckel [Bitter kola] Seed
INTERNATIONAL JOURNAL OF ADVANCES IN PHARMACY, BIOLOGY AND CHEMISTRY Research Article Comparism of the Antimicrobial Activities of N-hexane and Diethyl Ether Extracts of Garcinia kola Heckel [Bitter kola]
More informationANTIBACTERIAL ACTIVITY OF GYMNEMA SYLVESTRE HYDROALCOHOLIC LEAF EXTRACT.
International Journal of Advanced Research and Review www.ijarr.in ANTIBACTERIAL ACTIVITY OF GYMNEMA SYLVESTRE HYDROALCOHOLIC LEAF EXTRACT. Dr.Mayuri Thanwar 1, Dr.Dhananjay Dwivedi 2 1. Scientific Officer,
More informationAntibacterial activities of extracts and their fractions of leaves of Tridax procumbens Linn
Antibacterial activities of extracts and their fractions of leaves of Tridax procumbens Linn Dhasarathan. P 1, Hemalatha. N 2, Theriappan. P 1 and Ranjitsingh, A.J.A 3 1 Department of Biotechnology, Prathyusha
More informationActivity of Cassia fistula L. Barks fractions as antibacterial agent
Activity of Cassia fistula L. Barks fractions as antibacterial agent Chaerunisaa, Anis Yohana * ; Milanda, Tiana; Susilawati, Yasmiwar 1 Faculty of Pharmacy, Padjadjaran University, Jl. Raya Jatinangor
More informationPhytochemical Analysis and Antimicrobial Activities of Carissa carandas L.
Universities Research Journal 2011, Vol. 4, No. 1 Phytochemical Analysis and Antimicrobial Activities of Carissa carandas L. Aye Aye Naing Abstract The plant Carissa carandas L. locally known as Khan-pin,
More informationAntifungal activity of methanolic and ethanolic leaf extracts of medicinal plants
INTERNATIONAL JOURNAL OF PLANT PROTECTION VOLUME 9 ISSUE 2 OCTOBER, 216 474-478 e ISSN-976-68 Visit us : www.researchjournal.co.in IJPP RESEARCH PAPER DOI : 1.74/HAS/IJPP/9.2/474-478 Antifungal activity
More informationPhytochemical,Screening,of,Various,Extracts,of,Root,of,Withania, Somnifera*(L)*Dunal*
Archieves*of*Business*Research* *Vol.3,*No.2* Publication*Date:March25,2015 DOI:10.14738/abr.32.823. Vinotha,S.,Thabrew,I.,&Ranjani,S.S.(2015).PhytochemicalScreeningofVariousExtractsofRootofWithaniaSomnifere
More informationPRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO ANTIBACTERIAL ACTIVITY OF CUCURBITA MAXIMA SEED EXTRACT
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article PRELIMINARY PHYTOCHEMICAL SCREENING AND INVITRO ANTIBACTERIAL ACTIVITY OF CUCURBITA MAXIMA
More informationSTUDY ON THE ANTIMICROBIAL ACTIVITY OF THE CRUDE EXTRACT OBTAINED FROM THE ROOTS OF PLUMBAGO ZEYLANICA AND EVALUATION OF ITS MICROSPHERES
ISSN: 2230-7346 Research Article Journal of Global Trends in Pharmaceutical Sciences Vol.2, Issue 2, pp -212-221, April-June 2011 STUDY ON THE ANTIMICROBIAL ACTIVITY OF THE CRUDE EXTRACT OBTAINED FROM
More informationANTIMICROBIAL ACTIVITY OF LEAVES AND LATEX EXTRACT OF THE HERBAL PLANT CALOTROPIS GIGANTEA (ERUKKU IN TAMIL)
IJBPAS, April, 2012, 1(3): 261-270 ISSN: 2277 4998 ANTIMICROBIAL ACTIVITY OF LEAVES AND LATEX EXTRACT OF THE HERBAL PLANT CALOTROPIS GIGANTEA (ERUKKU IN TAMIL) MURUGAN T* Head, Department of Microbiology,
More informationEvaluation of antifungal activity of Zingiber officinale against Fusarium oxysporum f.sp. lycopersici
Available online at www.pelagiaresearchlibrary.com Advances in Applied Science Research, 2016, 7(2):5-9 Evaluation of antifungal activity of Zingiber officinale against Fusarium oxysporum f.sp. lycopersici
More informationAsian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:
Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: www.ajpamc.com FORMULATION AND EVALUATION OF HERBAL WASH FOR NASAL HEALTH
More informationInternational Journal of Ayurvedic and Herbal Medicine 1:1 (2011) 1 7
International Journal of Ayurvedic and Herbal Medicine 1:1 (2011) 1 7 Journal homepage: http://www.interscience.org.uk/index.php/ijahm Preliminary phyto chemical screening and anti microbial activities
More informationA Study on Phytochemicals, Antioxidant, Antidiabetic and Antimicrobial Activity of the Leaves of Solanum Trilobatum
RESEARCH ARTICLE A Study on Phytochemicals, Antioxidant, Antidiabetic and Antimicrobial Activity of the Leaves of Solanum Trilobatum Fabiola 1, Dr. V. Judia Harriet Sumathy 2 PG & Research Department of
More informationIsolation of Herbal Plants: Antifungal and Antibacterial Activities
Isolation of Herbal Plants: Antifungal and Antibacterial Activities Rathi Sanjesh G 1*, Patel Kanu R 2, Bhaskar Vaidhun H 3 1. Shri JagdishPrasad Jhabarmal Tibrewala University, Vidyanagari, Jhunjhunu,
More information3. PRELIMINARY PHYTOCHEMICAL SCREENING
93 3. PRELIMINARY PHYTOCHEMICAL SCREENING 3.1 INTRODUCTION All the drugs- Ayurvedic, Unani and Herbal extracts were subjected to preliminary phytochemical screening to test the presence of alkaloids, carbohydrates
More informationComparative Physicochemical and Phyto-Chemical Screening of Glucomap: An Herbal Antidiabetic Tablet
Comparative Physicochemical and Phyto-Chemical Screening of Glucomap: An Herbal Antidiabetic Tablet Satyaendra K. Shrivastava* 1, P.K. Dubey 1, B. Shrivastava 2, Pankaj Sharma 2 * 1 Swami Vivekanand College
More informationIN VITRO ANTIMICROBIAL ACTIVITY OF VARIOUS EXTRACTS OF MIRABILIS JALAPA LEAVES
Int. J. Chem. Sci.: 8(1), 2010, 559-564 IN VITRO ANTIMICROBIAL ACTIVITY OF VARIOUS EXTRACTS OF MIRABILIS JALAPA LEAVES R. MEERA *, P. DEVI a, P. MUTHUMANI, B. KAMESWARI b and B. ESWARAPRIYA c Department
More informationAvvaiyar govntment College for women, Karaial U.T of Puducherry. India.
ANTIMICROBIAL EFFECT OF MEDICINAL PLANT PEDALIUM MUREX AGAINST DIFFERENT MICROORGANISMS Anandanayaki S 1 Uma C 2* 1 Department of Plant science, 2 Department of Chemistry, Avvaiyar govntment College for
More informationISSN: Available online Journal of Global Trends in Pharmaceutical Sciences Vol.2, Issue 3, pp , July -Sept 2011
Research Article ISSN: 2230-7346 Available online http://www.jgtps.com Journal of Global Trends in Pharmaceutical Sciences Vol.2, Issue 3, pp -290-295, July -Sept 2011 PRELIMINARY PHYTOCHEMICAL, PHYSICOCHEMICAL
More informationBioautography assay of Caesalpinia coriaria (Jacq) wild, as antifungal agent.
INTERNATIONAL JOURNAL OF CURRENT RESEARCH IN BIOLOGY AND MEDICINE ISSN: 2455-944X www.darshanpublishers.com DOI:10.22192/ijcrbm Volume 1, Issue 6-2016 Original Research Article Bioautography assay of Caesalpinia
More informationFormulation of a Carbonated Herbal Health Drink with Hemidesmus indicus root extract
International Journal of Advance Industrial Engineering E-ISSN 2320 5539 2018 INPRESSCO, All Rights Reserved Available at http://inpressco.com/category/ijaie/ Research Article Formulation of a Carbonated
More informationScreening of phytochemical and antibacterial activity of Hemidesmus indicus (L.) and Vetiveria zizanoides (L.)
Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 2012, 2 (2):363-368 ISSN: 2248 9215 CODEN (USA): EJEBAU Screening of phytochemical and antibacterial activity
More informationAntimicrobial activity of Trinpanchmool drugs
Available online at www.scholarsresearchlibrary.com Scholars Research Library Archives of Applied Science Research, 2010, 2 (3): 183-187 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-508X
More informationPhytochemical Analysis and Antioxidant property of Aegle marmelos Extracts
ISSN: 2319-7706 Volume 4 Number 9 (2015) pp. 826-830 http://www.ijcmas.com Original Research Article Phytochemical Analysis and Antioxidant property of Aegle marmelos Extracts Anjay Kumar Gupta*, Sumeet
More informationCASSIA FISTULA SPECIES BY DFT APPROACH
COMPUTATION OF BINDING ENERGY OF BIOACTIVE CONSTITUENTS PRESENT IN CASSIA FISTULA SPECIES BY DFT APPROACH G.Valli* and M.Suganya The Standard Fireworks Rajaratnam College for Women, Sivakasi-626123 Tamilnadu,
More informationQuantitative estimation of Secondary Metabolite and Inhibitory effect of Azima Tetracantha leave extract against Candida Albicans
Quantitative estimation of Secondary Metabolite and Inhibitory effect of Azima Tetracantha leave extract against Candida Albicans Sandhiya.V* 1, Kavitha.C 2 1 * Department of Pharmaceutics, C.L. Baid Metha
More informationJournal of Medicinal Plants Studies Preliminary Phytochemical Studies Of Kalanchoe pinnata (Lam.) Pers
Year: 2013, Volume: 1, Issue: 2 First page: (19) Last page: (23) ISSN: 2320-3862 Online Available at Journal of Medicinal Plants Studies Preliminary Phytochemical Studies Of Kalanchoe pinnata (Lam.) Pers
More informationVol - 4, Issue - 1, Jan 2013 ISSN: Sharma et al PHARMA SCIENCE MONITOR
PHARMA SCIENCE MONITOR AN INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES COMPARATIVE ANTI BACTERIAL ACTIVITY OF LAGENARIA SICERARIA, MOMORDICA CHARANTIA AND CHENOPODIUM AMBROSIOIDES AGAINST CERTAIN HUMAN
More informationPhytochemical analysis and antifungal activity of Cauliflower stem (Brassica oleraceae var botrytis L.)
Phytochemical analysis and antifungal activity of Cauliflower stem (Brassica oleraceae var botrytis L.) Gowri, G 1 and Manimegalai, K 2 1,2 Department of Zoology, Avinashilingam Institute for Home Science
More informationPreliminary Phytochemical Screening Analysis and Therapeutic Potential of Tecoma stans (L.)
International Journal of Applied Chemistry. ISSN 0973-1792 Volume 13, Number 1 (2017) pp. 129-134 Research India Publications http://www.ripublication.com Preliminary Phytochemical Screening Analysis and
More informationNSave Nature to Survive
ISSN: 0973-7049 NSave Nature to Survive : Special issue, Vol. 1; 89-94; 2010 EVALUATION OF ANTIBACTERIAL POTENTIAL OF STEM BARK OF MORINGA OLEIFERA LAM Renu Sarin et al. Moringa oleifera Antimicrobial
More informationCOMPARATIVE ANTI MICROBIAL STUDY OF SHUDDHA KASISA AND KASISA BHASMA
Research Article International Ayurvedic Medical Journal ISSN:2320 5091 COMPARATIVE ANTI MICROBIAL STUDY OF SHUDDHA KASISA AND KASISA BHASMA Dr. Nisha Kumari.P. R *Dr. Dinesh Nayak J **, Dr. Sathyanarayana
More informationCONTROL OF RICE BLIGHT PATHOGEN XANTHOMONAS ORYZAE THROUGH HERBAL PLANT EXTRACT
IJPSR (2014), Vol. 5, Issue 12 (Research Article) Received on 30 April, 2014; received in revised form, 09 July, 2014; accepted, 08 August, 2014; published 01 December, 2014 CONTROL OF RICE BLIGHT PATHOGEN
More informationAntimicrobial activity of some medicinal plants against multidrug resistant skin pathogens
Journal of Medicinal Plants Research Vol. 5(16), pp. 3856-3860, 18 August, 2011 Available online at http://www.academicjournals.org/jmpr ISSN 1996-0875 2011 Academic Journals Full Length Research Paper
More informationAvailable online at
Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 2013, 3(2):107-111 ISSN : 2249-7412 CODEN (USA): AJPSKY Comparative study on in vitro antibacterial and antifungal
More informationAntimicrobial Activity and Phytoichemical Screening of Ximenia americana L Bark and Leaves
Antimicrobial Activity and Phytoichemical Screening of Ximenia americana L Bark and Leaves Zeinab M. M. Ali 1 Ahmed. E. M Saeed 2 and Hassan. S. Khalid 3 1 Department of Chemistry, College of Science,
More informationAntimicrobial activity and phytochemical analysis of Calotropis gigantea root, latex extracts. Pramila kori* and 2, Prerana alawa
IOSR Journal Of Pharmacy (e)-issn: 2250-3013, (p)-issn: 2319-4219 www.iosrphr.org Volume 4, Issue 6 (June 2014), PP. 07-11 Antimicrobial activity and phytochemical analysis of Calotropis gigantea root,
More informationNdukwe, I. G.*, Bello, A. I., Habila, J. D. and John, C. Department of Chemistry, Ahmadu Bello University, Zaria, Nigeria. Accepted 30 August, 2007
African Journal of Biotechnology Vol. 6 (23) pp. 2645-2649, 3 December, 2007 Available online at http://www.academicjournals.org/ajb ISSN 1684 5315 2007 Academic Journals Full Length Research Paper Phytochemical
More informationIn vitro Antimicrobial Activity of Extracts from Careya arborea Roxb Leaves
Microbiology Journal 5 (): 7-20, 205 ISSN 253-0696 / DOI: 0.3923/mj.205.7.20 205 Academic Journals Inc. In vitro Antimicrobial Activity of Extracts from Careya arborea Roxb Leaves 2 Mahadev R. Mali and
More informationResearch Article. Phytochemical and antibacterial efficacy of Hevea brasiliensis
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2015, 7(12):777-783 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Phytochemical and antibacterial efficacy of Hevea
More information*Kareem, S. O.; Akpan, I. and Ojo, O. P. Department of Microbiology, University of Agriculture P. M. B. 2240, Abeokuta, Nigeria
African Journal of Biomedical Research, Vol. 11 (2008); 105-110 SSN 1119 5096 badan Biomedical Communications Group Full Length Research Article Antimicrobial Activities of Calotropis procera on Selected
More informationStudies on biodeterioration of some chemical constituents in fresh and market roots of drug Desmodium gangeticum DC. under storage
Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 2013, 3(1):161-165 ISSN: 2248 9215 CODEN (USA): EJEBAU Studies on biodeterioration of some chemical constituents
More informationPhytochemical and antibacterial activities of crude leaf and root extracts of Clitoria ternatea varieties (Fabaceae)
2017; 6(6): 1104-1108 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2017; 6(6): 1104-1108 Received: 12-09-2017 Accepted: 13-10-2017 Rajesh Kumar T Santhosh Kumar R Anju VS Correspondence Rajesh Kumar T Phytochemical
More informationANTI-MICROBIAL ACTIVITIES OF BETEL NUT (ARECA CATECHU LINN) SEED EXTRACTS
ANTI-MICROBIAL ACTIVITIES OF BETEL NUT (ARECA CATECHU LINN) SEED EXTRACTS Ajima Karphrom 1, Suthisak Suknaisilp 2, Pasawadee Pradeepasaena 2, Sumate Tantratian 2 * ABSTRACT Seeds of betel nuts grown in
More informationPhytochemical Screening of Tubers and Leaf extracts of Sagittaria sagittifolial.:newsa (Arrowhead)
International Journal of Scientific and Research Publications, Volume 7, Issue 9, September 2017 431 Phytochemical Screening of Tubers and Leaf extracts of Sagittaria sagittifolial.:newsa (Arrowhead) Anita
More informationJournal of Chemical and Pharmaceutical Research
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(5):420-424 Screening of invitro antimicrobial activity
More informationThe textile material is goods carrier of various types
AJHS Asian Journal of Home Science Volume 9 Issue 1 June, 2014 308-312 e ISSN-0976-8351 Open Access - www.researchjournal.co.in A Case Study Extraction and application of antimicrobial agents using neem
More information*MIAN SHAHZADA ZIA AHMAD & ZAHEER-UD-DIN KHAN. Department of Botany, GC University, Lahore. ABSTRACT INTRODUCTION
BIOLOGIA (PAKISTAN) 01, 8 (1&), 17-17 PK ISSN 000-09 Antibacterial activity of crude s of different parts of Butea monosperma (Lamk.) Taub. *MIAN SHAHZADA ZIA AHMAD & ZAHEER-UD-DIN KHAN Department of Botany,
More informationCHAPTER 6 EVALUATION OF SELECTED PLANT EXTRACTS FOR EVALUATION OF SELECTED PLANT EXTRACTS FOR ANTI-ACNE ACTIVITY
CHAPTER 6 EVALUATION OF SELECTED PLANT EXTRACTS FOR School of Science, SVKM s NMIMS University Page 119 6. EVALUATION OF SELECTED PLANT EXTRACTS FOR 6.1 MATERIALS AND METHODS 6.1.1 Antimicrobial assays
More informationEvaluation of Antibacterial and Antifungal Activities of Leaf and Seed Extracts of Croton Tiglium Plant against Skin Disease Causing Microbes
International Journal of Research Studies in Biosciences (IJRSB) Volume 3, Issue 5, May 2015, PP 139-144 ISSN 2349-0357 (Print) & ISSN 2349-0365 (Online) www.arcjournals.org Evaluation of Antibacterial
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research J. Chem. Pharm. Res., 2010, 2(2): 107-111 ISSN No: 0975-7384 Phytochemical Screening of Curculigo Orchioides Gaertn. Root
More informationEvaluation of Diuretic Activity of Jussiaea Suffruticosa Linn.
Evaluation of Diuretic Activity of Jussiaea Suffruticosa Linn. R.Mythreyi 1, N.Rajkumar 1* and E.Sasikala 2 1 Maharaji College of Pharmacy, Besant Nagar, Chennai-90. 2 Central Research Institute for Siddha,
More informationTHE EFFECT OF ALKALOIDS AND FLAVONOIDS EXTRACTS OF VITEX DONIANA SEED ON SOME MICROORGANISMS
THE EFFECT OF ALKALOIDS AND FLAVONOIDS EXTRACTS OF VITEX DONIANA SEED ON SOME MICROORGANISMS E.I. Okoye Department of Pure and Industrial Chemistry, Anambra State University, Uli, Nigeria ABSTRACT: This
More informationPhytochemical and Anti-cholestrol activity of Aegel marmelos (L.) Corr. leaf extract using in albino mice
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Special Issue-3 (February-2016) pp. 82-87 Journal homepage: http://www.ijcmas.com Original Research Article Phytochemical
More informationAntimicrobial activity of Terminalia chebula
, ISSN 2249 4340 Vol. 1, No. 2, pp. 175-179, September 2011 RESEARCH ARTICLE Antimicrobial activity of Terminalia chebula M. Golam MOSTAFA, Mahdia RAHMAN, M. Manjurul KARIM* Department of Microbiology,
More informationPhytochemical Analysis, Antimicrobial Efficacy and Determination of Bioactive Components from Leaves of Justicia Adhatoda (Linn)
Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 2015, 5(2): 43-51 ISSN : 2249-7412 CODEN (USA): AJPSKY Phytochemical Analysis, Antimicrobial Efficacy and
More informationAnti-microbial Properties of Thai Traditional Flower Vegetable Extracts
Anti-microbial Properties of Thai Traditional Flower Vegetable Extracts Wunwisa Krasaekoopt and Areeya Kongkarnchanatip Faculty of Biotechnology, Assumption University Bangkok, Thailand Abstract In this
More informationAntimicrobial activity of fabrics treated with Quercus Infectoria extract for foot odour control. V.Yamuna, S.Sudha*
ISSN : 0974-7435 Volume 7 Issue 6 Antimicrobial activity of fabrics treated with Quercus Infectoria extract for foot odour control V.Yamuna, S.Sudha* Department of Fashion Technology, PSG College of Technology,
More informationInternational Journal of Food Nutrition and Safety, 2012, 1(2): International Journal of Food Nutrition and Safety
International Journal of Food Nutrition and Safety, 2012, 1(2): 54-59 International Journal of Food Nutrition and Safety Journal homepage: www.modernscientificpress.com/journals/ijfns.aspx ISSN: 2165-896X
More informationScreening Of Phytochemical And Antibacterial Activity Of Polygala javana Plant Leaf, Stem And Root Extract Against Human Pathogen
International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.4, No.4, pp 1792-1796, Oct-Dec 2012 Screening Of Phytochemical And Antibacterial Activity Of Polygala javana Plant Leaf,
More informationPharmacognostic Study Of Plant Euphorbia hirta L
Pharmacognostic Study Of Plant Euphorbia hirta L Research Article Giram Ganesh Bhagwat 1 *,Chaulang Ganesh Mahadev 1,Ghodke Dhananjay Sahebrao 1, Patil Kundan Suresh 1,Yeole Pramod Govindrao 1,Ganjiwale
More informationReceived: 20 th April-2013 Revised: 28 th April Accepted: 29 th April Research article
Received: 20 th April-2013 Revised: 28 th April -2013 Accepted: 29 th April -2013 Research article EVALUATION OF ANTIMICROBIAL ACTIVITY OF EUPHORBIA HIRTA AND CALOTROPIS PROCERA AGAINST MDR PATHOGENS 1
More information