PREPARTION AND EVALUATION OF MULTIPURPOSE HERBAL CREAM

Transcription

1 PREPARTION AND EVALUATION OF MULTIPURPOSE HERBAL CREAM N R Patel*, H U Momin, R L Dhumal, K L Mohite Faculty of Pharmacy, Adarsh College of Pharmacy Vita. Maharashtra ABSTRACT Objectives To improve the beauty of the human, herbal cosmetic preparation are used. The objective of present research was to formulate and evaluate multipurpose herbal cream for the purpose of soothing, nourishing, Moisturizing, And Emollient and to treat various skin diseases. Material and method The herbal extracts are prepared by using hydroaclcholic extract method (Papaya, Green tea, Chandan) and by aqueous extract method (Aloe, Tulsi, Neem and Turmeric) of crud drug. Different crude drug like Aloe (Aloe barbadensis), Papaya, (Carica Papaya), Turmeric (Curcumalonga), Tulsi (Ocimum sanctum), Neem (Azadirachtaindica), Chandan (Santalum album), Green tea (Camellia Sinesis), Rose oil (Olium rosae), Almond oil (Prunus dulcis), Lemon oil (cymbopogon flexuosus ) were taken. RESULT & DISCUSION- For the formulation of cream the Evaluations all these Formulations were dones on different parameter like P H of cream, viscosity, Homogenicity, Appearance, Acid value, Saponification value. Washability, Irritancy, Consistency & Antimicrobial growth test. The Formulation showed good result of all above parameters. CONCULSION Antimicrobial testing of formulation has been concluded that both the formulation gives antimicrobial activity toward Gram positive (Bacilus) as well as Gram negative (E.coli) bacteria. Thus these studies suggest that all the herbal cosmetic preparation is safe to us was provided & it can be used as a provision of protective barrier to skin & also it may produce Synergistic action. KEY WORD Anti-acne, Antimicrobial, Camellia Sinesis, Cosmaceutical, Multipurpose herbal cream. Corresponding Author: *N R Patel Faculty of Pharmacy, Adarsh College of Pharmacy Vita. Maharashtra INTRODUCTION The word Cosmetic derived from a Greek word kosmesticos that means to adorn. From that time any materials used to beautification or promoting appearance is known as cosmetic various types of natural material were used for these purpose. [1] Herbal excipients are of plant origin extracted & isolated from plant parts. Being natural herbal excipients are non toxic, affordable, biodegradable with some exception, biocompatible, ecofriendly & can be changed chemically thus attract the consumers. The plant sources are renewal & can be cultivated or harvested in sustainable manner, can produce constant availability of raw material, waste from food industry can be achieved as a raw material to extract herbal excipients. These are other reasons for improve in demand of herbal material as a excipients. The basic idea for skin care cosmetic lies deep in the Rigveda, Yajurveda, Ayurveda, Unani & Homeopathy system of medicine. [2] These are the product in which herbs are used in crud or in extract form. These herbs should have different properties like Advanced Journal of Pharmacie and Life science Research 27

2 antioxidant, anti-inflammatory antiseptic emollient, Antiseborrhatic antikerolytic and Antibacterial etc. cosmetic are prepared or formulate to reduce wrinkles. Fight acne & to control oil secretion for various types of skin protective, sunscreen, Antiance, Antiwrinkle & antiaging & designed using different types of materials either natural or synthetic. Multipurpose herbal cream is a poly herbal preparation that contains of extracts of Aloe barbadensis, Ocimum sanctum, Azadirachtaindica, curcuma longa, Olium rosae, Carica papaya, Santalum album, camellia Sinesis, prunus dulcis, cymbopogon Flexuosus. These herbs have been selected depend on value of herbal in traditional system. [3] PREPARATON AND EXTRACT- Two method are used for Preparation of extract 1. Aqueous extracts For (Aloe, Turmeric, Neem &Tulsi) All ingredient are weighed accurately & aqueous extract had been done i.e. 5gm in 50 ml water on water bath at c. This solution concentrated up to 20ml. Filtrations is done same procedure is followed on all powder. [4,5] 2. Alcoholic extracts for (papaya, Chandan & green Tea) All ingredient weight accurately & prepare alcoholic extract has be done. i.e. 5gm in 20ml alcohol & 30ml water heat on water bath at c. This solution concentrate up to 20ml Filtration is done, same procedure is followed on all powder.(fig1) FORMULATON PREPARATION- The oil in water (o/w) emulsion based cream was formulated. The emulsifier stearic acid & cetyl alcohol (part A) dissolved & heated at 75 0 c. The preservatives & other water soluble component (methyl paraben, propyl paraben, Trienthanolamine, propylene glycol) & all extract dissolved in aqueous phase (part B). After heating aqueous phase was added into oil phase at same temperature with continuous stirring the smooth & homogenous cream was prepared. After cooling then add perfume. The formula for [6, 7] given. (Table 1) Table No 1- Formulation of cream Srn o Ingredient F1 % F2 % F3 % 1 Aloe barbadensis Carica papaya Camellia Sinesis Curcuma longa Azadirachta indica Santalum album Ocimum sanctum Stearic acid Cetyl alcohol Methyl paraben Propyl paraben Trienthanolamine Propylene glycol Water q.s q.s q.s 15 Perfume Rose oil q.s Almond oil - q.s - 17 Lemon grass oil - - q.s EVALUATIN OF CREAM [8, 9, 10] The Evaluation of cream as follow 1. Physical Properties: - The cream was observed physical appearance like color, odour & texture. Advanced Journal of Pharmacie and Life science Research 28

3 2. Homogenenicity: - The formulation was tested for the homogenicity by visual appearance & by touch. 3. After feel: - Emliency slipperiness & amount of residue lest after the application of fixed amount of cream was checked. 4. Wash ability: a small amount of cream applied on hand & washed under running tap water. 5. Consistency: This parameter was checked manually. 6. ph of cream: - P H was calibrated using standard butter solution. About 0.5 gm of cream was dissolved in 50ml of Distilled water to measured P H. 7. Viscosity: - Viscosity of formulated cream was determined by book field viscometer at 100 rpm using spindle No Irritancy: - Mark an area (159.cm) on left hand dorsal surface. The formulated cream was applied particular area & time was noted. Irritancy, Erythrema, Edema check on 24hr. 9. Acid value: gm of cream adds in10ml of alcohol & 10ml & ether warm water bath until it dissolve. Titrate against 0.1N sodium hydroxide. Using phenolphthalein as indicator let titare reading v X 0.1x2x v3 V3 = titre reading 10. Saponification Value: - 0.5gm of cream transfer into round bottom flask add 15ml of alcoholic pot hydroxide & reflux on boiling water bath for about an hr (v1). 15ml of alcoholic pot hydroxide reflux on boiling water bath for about an hr (v2). Cool the both solution in to room temp & titrate separately against 0.5N hydrochloric acid using phenolphthalein as the indicator. Let us the titre reading for sample as v1 & black is v2. 2(V 2 -V 1 ) V 2 = Blank reading V 1 =Sample reading 11. Microbial growth test: - The Formulated cream was prepared were inoculated on the agar media streak plate & agar plate method & a control was prepared by omitting the cream. The plate was place in incubator are incubate 37 0 c for 24hr. [3] 12. Stability testing:- Stability testing of prepared formulation was conducted for most stable formulation room temp studied for 7day s. The formulation were kept both at room temp & Evaluated temperature observed on 0th, 10th,15th & 20th day for following parameter. RESULT AND DISCUSIION 1. Physical Properties of Cream- The physical properties & all formulated cream were judged by its color. Odour & texture. The results are tabulated below. Table No.2 Physical Properties of cream: Sr. No F1 F2 F3 1 pale yellow pale yellow pale yellow 2 characteristic characteristic characteristic 3 semi-solid semi-solid semi-solid 2. ph of cream- The result of P H of prepared cream was found to be around 6 which are suitable for topical application. Because skin P H in between The result of P H summarized in table No. 3 Advanced Journal of Pharmacie and Life science Research 29

4 Adv J Pharm Life sci Res, 20175; ;1:27-32 ISSN (On-line) Table No.3 ph of cream: Sr. No Formulation 1 F1 2 F2 3 F3 3. Viscosity The viscosity of cream was found in range of 500 to 100cp which indicate that cream is easily spreadable by small amount of shear. The result is tabulated below. Table No.4 Viscosity of cream: Sr. Viscosity of cream (cp) Room temp No F1 F2 F Irritancy All formulation shows no irritation, Erythrema & edema during Irritancy test study. The resulted of Irritancy test were formulation are safe to use for skin. The results are shown in below table No. 5 Table No.5 Irritancy of cream: Sr. No Formulation Irritant Erythrema Edema 1 NIL NIL NIL 2 NIL NIL NIL 3 NIL NIL NIL P H NIL NIL NIL 5. Formulation applied for acid value & Saponification Value - The result of acid value & Saponification value all formulation creams were presented in Below table & showed satisfactory values. Table No. 6 Acid value and saponification Value: Sr. Parameter formula No. F1 F2 F3 1 Acid Value Saponification value Microbial growth test The formulated cream were tested for the presence of gram positive (bacillus) & gram negative (E.coli) agar medium nutrient agar & Mackony agar respectively show in. There were sign of microbial growth after incubation period of 24hrs at 37 0 c. This indicates cream show the antimicrobial activity (Fig2, 2.1, and 2.1) 7. Stability test- The Formulated cream showed almost constant PH homogenous, emollient, non grassy & easily removed after the application our study indicate that the formulation F1 & F3 found be stable while F2 remaining Formulation was not stable & result is change in colour when stored for long time in Table NO.7 Table No. 7 Stability test of the cream: Days T # F ## PH X1 X2 X3 X4 X5 0 RT F1 6.1 NCC ** E EW ** F2 6.5 NCC ** E EW ** F1 6.2 NCC ** E EW ** 5 RT F2 6.4 NCC ** E EW ** F1 6.2 NCC ** E EW ** 10 RT F2 6.4 NCC ** E EW ** F1 6.1 NCC ** E EW ** 15 RT F2 6.4 NCC ** E EW ** 20 RT F1 6.2 NCC ** E EW ** F2 6.5 NCC ** E EW ** #Temperature, ## Formulation, X1 = Appearance, X2= Homogenicity,X3= after feel, X4 = Washability, X5 = Consistency, **-Good, *- Satisfactory, NCC- Not Change color, E= Emollient, ES= Easy wash ability. Fig No.1. Different types of Herbal Cearm Advanced Journal of Pharmacie and Life science Research 30

5 (On Adv J Pharm Life sci Res, ;1: ;1 this cream can be used as multipurpose cream & the extracts of herbal drug produce synergistic effect of the other. The prepare Formulation ormulation showed good antimicrobial activity no evidence of phase separation & good consistency during the study period. ACKNOWLEDGEMENT Fig No.2. Microbial growth test Different types of Herbal Cearm The authors are grateful to the management of Adarsh College of pharmacy vita for providing all the necessary facilities for the successful completion of this work. REFERANCES Fig No.2.1. Microbial growth test Different types of Herbal Cearm Fig No.2.1. Microbial growth test Different types of Herbal Cearm CONCULSTION Multipurpose herbal cream was o/w type emulsion. This emulsion based cream was formulated using herbal extracts & was evaluated. This work can be concluded that 1. R.N Shah, B.M Methal, A, Hand book of Cosmetic Page No Khawenkars, Bhatiav, Shashtrik, yadav M & Pandeys. Herbal excipietns: pharmaceutical application. International Journal of pharmacognosy& ognosy& phytochemical Research 2014: 6(3) : Akash.Mali, Karekar P. D r & Yadav A.V. Formulation & Evaluation of Multipurpose Herbal cream. Internation Journal Science & Research (IJSR): Moorthyk, Aparna, Aravind & Punit T. In Vitro Screening ning of antimicrobial activity of Wrightia tinctoria (ROxB)R Br. Asian Journal of pharmaceutical & clinical Research, X Fatima Grace, R Joan vijetha, S Shanmuganathan, & D Chamundeeswari. Formulation & Evaluation of Polyherbal cosmetic cream. Adv. J pharma like of poly Sci Res, , Katiyarsk, Ahmad N. Mukhtar H. Green tea & skin. Arch Dermatol 2000; 136: : Ashawat M.S, Saraf Shailendra & Saraf Swarnlata. Biochemical & histopathological studies of herbal cream againt uv radiation. Advanced Journal of Pharmacie rmacie and Life science Research 31

6 8. Lachman L. Liebermah HA & Kanig JL. The theory & practice of industrial pharmacy 3 rd edn, USA, Lea & Febiger. 9. H.N more, K.A.Hajare, Practical physical pharmacy, second edition Mandeepsingh, Shalinisharma, Sukhbir Lal khokra, & Ram kumarsahy, preparation & Evaluation of herbal cosmetic cream. Pharmacology online 2; (2011) ******** Advanced Journal of Pharmacie and Life science Research 32