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1 FDA Compliant Patented & Patent Pending ISO 13485:2003 ISO 9001:2000

2 Embryo Corral & Embryo GPS TM IVF Dishware Specially Designed & Tested for IVF No more droplet collapsing, running and mixing. The benefits of the Embryo GPS TM and Embryo Corral designer dishware. Time savings, cost savings and major benefits to the oocyte and embryo. Benefit of less time consumption: Set-up time specifically designed wells make set up faster and easier. Observations time contoured well bottom and same focal distance make locating embryos quicker. Same dish configurations multiply the savings. This will result in time saving aspects for the embryologist. Benefit of possible cost reductions: Set-up costs specially designed dishes save in labor and time. Use of media predetermined dish design allows better planning for overall less media use. Less time needed for observation of embryos reduces overall costs and time spent on this task. All dishes are LAL and 1-cell MEA tested meaning greater confidence and less onsite testing. COA for each batch included with each shipment. Optimal for more sensitive procedures and PGD biopsies. Results in more consistent oocyte and embryo placement and savings in use and testing. Benefits to the oocytes and embryos: The overall design means safety in handling embryos. No likelihood of collapsing droplets. Fixed volumes per well and standardization. Rapid identification of oocytes/embryos at a fixed focal point (GPS). Allows better oil coverage by dish design. Grouping of embryos in the Embryo Corral may improve outcome. GPS dishes are sterile Gamma Radiated; LAL and 1-cell MEA tested by independent testing company. Bottom of dish allows better and uniform heat exchange. New lid allows greater gas exchange and may reduce likelihood of sealing by oil and untoward alteration of ph. A major benefit is that the embryos will spend less time outside the controlled environment of the incubator. Adaptable to different culture schemes. The Embryo GPS TM and Embryo Corral dishware have been painstakingly designed to help improve safety and make life simpler for everyone.

3 The Embryo GPS TM dishware with its GPS location system improves security and safety of embryos and reduces time in a drop-less environment No More Droplet running, collapsing, or mixing The Embryo GPS TM dishware gives you structured wells and observation enhancements that allow you to save time and effort and improves safety in handling embryos. A drop-less environment with designed wells, the GPS location for embryos with the same microscope focal distance. The GPS dishes provide even and better temperature transition and distribution for a safe and consistent workplace for IVF and embryos. The GPS location system provides you time savings and improved safety all the time. The 8 outer wells have walls to culture in a drop-less environment; no more droplet collapsing. All 8 wells have the GPS locator spot. Ease in set-up, identification, observation, handling and increased safety. Safety comes with the GPS system. Each embryo rests safely in each well. No more drops collapsing or mingling during transportation. The wells provide uniform media volume and focal distance. The wells make oil overlay much easier and faster. GPS culture system In the GPS culture dishes each embryo rests in an exact spot in each well and at the exact same focal distance. Each embryo will be found at the exact location saving time and effort in locating and observing embryos. Each batch is 1-cell Mouse Embryo Assay (MEA) and Limulus Amebocyte Lysate (LAL) tested by independent testing company. Each batch comes with Certificate of Analysis. The bottom: Thinner bottom for better heat exchange and space for patient identification. Embryo GPS TM dish has 3 large center wells for culturing, quick embryo locating and observation. New lid design allows greater gas exchange with posts to prevent oil-induced sealing. FDA Compliant ISO 9001:2000 ISO 13485:2003 GPS dishware are made with tested non-toxic medical grade plastic manufactured by Nalge Nunc International exclusively for SunIVF. Proven safe in IVF and ART culture. The Embryo GPS TM dish provides 11 exact locations (3 central wells + 8 outer wells). Embryo GPS The best dish for PGD cases.

4 The Embryo Corral THE ONLY CHOICE FOR GROUP CULTURE The Embryo Corral is the only culture dish that allows group culture of embryos while allowing individual evaluation of each embryo. The fence of vertical posts in each central well prevents the embryos from passing from one quadrant to another, while still allowing exchange of culture media and embryo-derived autocrine and paracrine growth factors to among the embryos in the 4 quadrants of the 2 central wells, assisting embryo development. The 8 outer wells have walls to culture in a drop-less society, media will not collapse. All 8 wells have the GPS locator spot. The same ease in set-up, identification, observation and handling and safety. Each central well has 4 quadrants for grouping embryos and keeping specific identification. The GPS system makes ease in locating, observation, handling and overall safety. Safety comes with the GPS system. Each embryo rests safely in each well. No more drops collapsing or mingling during transportation. The wells provide uniform media volume and focal distance. The wells make oil overlay much easier and faster. New lid design allows greater gas exchange with posts to prevent oil-induced sealing. FDA Compliant The bottom: Thinner bottom for better heat exchange and space for patient identification. ISO 9001:2000 ISO 13485:2003 Embryos are held in separate quadrants. Medium, with autocrine and paracrine facors, can circulate among the quadrants. Each batch is 1-cell Mouse Embryo Assay (MEA) and Limulus Amebocyte Lysate (LAL) tested by independent testing company. Each batch comes with Certificate of Analysis. GPS dishware are made with tested non-toxic medical grade plastic manufactured by Nalge Nunc International exclusively for SunIVF. Proven safe in IVF and ART culture. The Embryo Corral dish provides 16 exact locations (2 central wells with 4 quadrants each + 8 outer wells).

5 GPS DISH YOUR PGD SOLUTION GPS dishware is a unique and practical way to be responsible with your embryos when performing PGD procedure. The predetermined wells in the GPS dishware significantly lower the likelihood of mixing droplets, therefore, you re always able to identify each embryo. The features of the GPS dishware allows you to locate the embryo faster, making the procedure shorter so embryos will spend less time out of the controlled environment of the incubator. The GPS dishware s specially designed lid prevents the lid sealing and increased PH. Its thinner bottom allows more stable and better heat exchange. 1-cell MEA and LAL tested. FDA compliant. CE certified. Please call for a FREE sleeve of Embryo GPS dishes to see the benefits for PGD biopsy proocedures. Ordering/Sampling Information Product Description Quantity Part Number Embryo GPS TM Embryo Culture Dish The first dish designed solely for IVF. 50pk (10/sleeve) EGPS-050 Embryo GPS TM Embryo Culture Dish The first dish designed solely for IVF. 100pk (10/sleeve) EGPS-100 Embryo GPS TM Embryo Culture Dish The first dish designed solely for IVF. 500pk (10/sleeve) EGPS-500 Embryo Corral Embryo Culture Dish Optimum for embryo growth and group culture. 50pk (10/sleeve) EMBC-050 Embryo Corral Embryo Culture Dish Optimum for embryo growth and group culture. 100pk (10/sleeve) EMBC-100 Embryo Corral Embryo Culture Dish Optimum for embryo growth and group culture. 500pk (10/sleeve) EMBC-500 Enriched specially designed PGD Biopsy PGD Biopsy Media formulation. Ca & Mg free w/ sucrose and is a 20 ml LPGG-020 HEPES buffered solution. 8 week min. shelf life. 50 ml LPGG-050 What Our Customers Say As of January we switched to the Embryo Corral dishes made by SunIVF. We have not had any more problems with the spreading of media drops. We are using less oil and we have noticed an increase in our clinical pregnancy rate for the first six months of this year compare to the same time in previous years. Toni Di Berardino MSc, Laboratory Director, Mount Sinai Hospital, Toronto, ON, Canada Although spreading medium droplets is a phenomenon that many of us have faced thru our careers, the introduction of the Corral and the GPS dishes from IVFonline have eliminated that problem in our laboratory. In addition to the benefits of a fixed volume per well and rapid identification of the oocyte/embryos at a fixed focal point the dishes are very easy to prepare and use for culture. The benefits of the group culture quadrant in the Corral dishes have been remarkable. They are very adaptable to many culture schemes. Perhaps employing dishware specifically designed and tested for IVF is the answer to the droplet issues. Charlene Alouf, PhD, Laboratory Director, Crozer Chester Medical Center Fertility Center, PA, USA

6 GPS vs. Standard Microdrop Culture Comparison of Handling Times Improves safety and reduces time in setting up, observing and handling. 15 Time (minutes) GPS Microdrop Figure 1: Comparison of the estimated handling times required for GPS or standard microdrop culture of one dish of 5 embryos from Day 1 to Day 5, with a change to fresh medium on Day 3. The bars represent the times for: preparation of the dishes, set-up of the cultures, and daily evaluation of the embryos. The total handling time for microdrop culture is 32% more than the total time for GPS culture. (Values are based on the results presented in Rieger et al., 2007, Proc. 14th World Congress on IVF & 3rd World Congress on IVM, Montreal, Abstract P-1202) P < GPS Microdrop P = P < Time per dish (min) 2 1 P = P < P = Day 0 Prepare Dishes Day 1 Set-up Cultures Day 2 Day 3 Day 4 Day 5 Embryo Evaluation 0 Total Figure 2: The times required for set-up and embryo examination in microdrops and GPS microwells. GPS Microdrop 100 P = % of Zygotes P = Cleaved Day 2 Day 3 Day 4 Day 5 Morula Compacted Blastocyst Expanded Blastocyst Expanded Hatched Figure 3: Mouse embryo development in microdrops and GPS microwells.

7 7 winning features of the GPS dishes 1. Improves safety and security of the embryo/oocyte or specimen. 2. Each self-contained well maintains the droplets integrity. No more droplet collapsing, running or mixing. 3. Each well enables rapid identification of each embryo or specimen. 4. Contoured bottoms of each well provide predetermined location of specimens and better heat exchange. 5. GPS dishes provide single or group culture with the benefits of individual tracking. 6. Redesigned lid allows increased CO 2 exchange. 7. Easy setup and use. Reduce time of setup, locating and observing embryos. The Embryo Corral and the Embryo GPS TM The only culture dishes you will ever need A better choice your patients deserve References 1 Rieger, D.; Schimmel, T.; Cohen, J.; Cecchi, M. Comparison of GPS and standard dishes for embryo culture: set-up and observation times, and embryo development. 2007, Proc. 14th World Congress on IVF & 3rd World Congress on IVM, Montreal, Abstract P Wiley, LM, et al Effect of potassium concentration, type of protein supplement, and embryo density on mouse preimplantation development in vitro. Fert Steril. 45(1): Paria, BC and Dey, SK Preimplantation embryo development in vitro: Cooperative interactions among embryos and role of growth factors. Proc Natl Acad Sci. 87: Canseco, RS, et al Embryo density and medium volume effects on early murine development. J Assist Reprod & Gen. 9(5): Lane, M and Gardner DK Effect of incubation volume and embryo density on the development and viability of mouse embryos in vitro. Hum Reprod. 7(4): Gardner, DK, et al Enhanced rates of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum and somatic cells: Amino acids, vitamins, and culturing embryos in groups stimulate development. Biol Reprod. 50: Salahuddin, SW, et al. Effects of embryo density and co-culture of unfertilized oocytes on embryonic development of in vitro fertilized mouse embryos. Hum Reprod. 10(9): O Neill, C Autocrine Mediators are required to act on the embryo by the 2-cell stage to promote normal development and survivial of mouse preimplantation embryo in vitro. 58: Gil, MA, et al Effect of volume of medium and number of oocytes during in vitro fertilization on embryo development in pigs. 60: de Oliveira, AT et al Gene expression and developmental competence of bovine embryos produced in vitro under varying embryo density conditions. Theriogenology. 64(7): Pickering, SJ et al An analysis of multinucleated blastomere formation in human embryos. Hum Reprod. 10(7): Balakier, H and Cadesky, K The frequency and developmental capability of human embryos containing multinucleated blastomeres. Hum Reprod. 12(4): Jackson, KV, et al Multinucleation in normally fertilized embryos is associated witha an accelerated ovulation iduction reponse and lower implantation and pregnancy rates in in vitro fertilization-embryo transfer cycles. Fert Steril. 70(1): Sakkas, D, et al Early cleavage of human embryos to the two-cell stage after intracytoplasmic sperm injection as an indicator of embryo viability. 13(1): Scott, L et al The morphology of human pronuclear embryos is positively related to blastocyst development and implantation. 15(11): Van Royen, E, et al Multinucleation in cleavage stage embryos. Hum Reprod. 18(5): Hnida, C et al Computer-controlled, multilevel, morphometric analysis of blastomere size as a biomarker of fragmentation and multinuclearity in human embryos. 19(2): Paula J. Stokes, Lalantha R. Abeydeera and Henry J. Leese, Development of porcine 1embryo in vitro and in vitro: evidence fro embryo cross talk in vitro. Dev Biol Aug 1;284: Alouf, C Technology in the IVF Laboratory: Group Culture Revisited. Fertility Vol.5: Norwich St. E. Guelph, ON, Canada N1H 2G6 gps@ivfonline.com US/Canada: Intl.: Fax:

8 Traditional Petri Dish Embryo GPS TM Dish running droplets droplets mixing droplets flattening poor temperature control losing track of marked droplet difficulty locating embryos sealing of dish lid & increased ph drop-less environment micro wells designed to enhance embryo culture no droplets mixing save time and money by reducing set up time, observation time, testing and handling better heat conservation enhance safety easily locate & observe embryos new designed lid standardization of culture, uniform volumes Standardize IVF culture by changing to the Embryo GPS TM Eliminate these concerns and enhance safety by changing to the Embryo GPS TM dishware. Embryo GPS The best dish for PGD cases. Tel: Tel: Fax:

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