A Microfluidic Culture Model of the Human Reproductive Tract: Screening of Female Reproductive Toxic Chemicals
|
|
- Cecilia Mason
- 6 years ago
- Views:
Transcription
1 A Microfluidic Culture Model of the Human Reproductive Tract: Screening of Female Reproductive Toxic Chemicals Shuo Xiao, PhD Assistant Professor Reproductive Health & Toxicology Laboratory Department of Environmental Health Sciences Arnold School of Public Health, University of South Carolina
2 Conflict of Interest Statement This is no conflict of interest for the work I present today.
3 Female Reproductive System Fallopian tube Menstrual cycle Ovary Cervix Ovary Follicles Uterus Vagina Infertility IVF
4 Female Reproductive Tract and Pregnancy Embryo development & transport Fertilization Primordial follicle Corpus Luteum Embryo implantation Primary follicle Secondary follicle Antral follicle Ovulation
5 Hormone Regulation of Female Reproductive System Highly regulated by pituitary hormones follicle-stimulating hormone (FSH) and luteinizing hormone (LH), and ovarian hormones Estrogen and Progesterone. Targets for endocrine disrupting chemicals (EDCs), pharmaceutical chemicals
6 Summary of Female Reproductive Toxicity Testing In vivo animal testing as a standard to test chemicals female reproductive toxicity Organ weight, morphology, and histology Vaginal smear and serum hormone levels Ovulation and oocyte production Fertility, pregnancy, litter size, multiple generational reproduction testing In vivo models are time-consuming, costly, and harmful to animals Lack of in vitro models for testing fallopian tube, uterus, and cervix In vitro 2D ovarian cell/follicle cultures do not phenocopy the physicochemical microenvironment and 3D tissue specific architecture Guidelines for Reproductive Toxicity Risk Assessment
7 Outline I. Using microfluidic system to establish the ex-vivo 28-day menstrual cycle hormone control and female reproductive tract on a chip - Establish II. Screening of female reproductive toxic chemicals - Discover
8 Organ-on-a-chip using Microfluidic Technology Brain chip Vessel chip Lung chip Heart chip
9 Clinical Research Only in Males Early testing of chemical toxicity in female is more difficult than in males because: Existence of menstrual cycle and hormone changes Lake of awareness of the importance of gender as a biological variable Ethical issues associated with women who have potential or current pregnancy Drugs have been withdrawn from the market for greater healthy risk in women Considering gender/sex as a biological variable in all NIH-funded research since 2015!
10 Hypothesis I. Develop ovarian functions in the microfluidic system to mimic human 28-day menstrual cycle hormone control and ovarian function II. Establish ex-vivo female reproductive tract (FRT) on a chip by integrating and inter-connecting multiple reproductive tissues
11 Microfluidic Platform (MPS) Design Passed media reservoir Tissue Modules Media donor Fluidic interface Tissue module Actuator interface Materials: can be sterilized, non-toxic to cells/follicles, do not bind hormones; Pumping pattern: computer program controlled. Xiao et al, Nat Commu, 2017
12 Ovary Chip Based on Microfluidic Culture Passed media reservoir Media donor Tissue module Sampling: easy handling and loading, collect the most recent passed media without disturbing a running culture. Xiao et al, Nat Commu, 2017
13 Ovary Chip Based on Microfluidic Culture Alginate encapsulation Follicle growth in vitro In vitro maturation Ovulation In vitro fertilization Embryo development Embryo transfer Live birth Apply the 3D in vitro follicle growth (IVFG) model in the microfluidic system, which mimics Xiao et al, Repro, 2015 full ovarian cycle (follicular phase, ovulation, luteal phase) Xu et al, Biomaterials, 2006
14 Follicle Maturation & Ovulation in Microfluidic Culture day 0 day 14 MII oocyte α-tubulin F-actin DAPI day 0 day 14 In vitro ovulation α-tubulin F-actin DAPI Microfluidic platform supports mouse ovarian follicle development and oocyte maturation. Xiao et al, Nat Commu, 2017
15 Follicle Luteinization upon hcg Stimulation Before hcg hcg 16h 48h 14 days 10 μm nucleus /1 mm Before hcg 24h after hcg Error bar: Standard deviation; *p<0.05 * 48h after hcg * 14 days after hcg The in vitro luteinization of follicular cells is similar to corpus luteum formation in vivo (cell hypertrophy and differentiation from granulosa cell to luteal cell). Xiao et al, Nat Commu, 2017
16 Ovarian Hormone Secretion in Microfluidic Culture Follicular phase Luteal phase Estradiol (nm) Estrodiol Progesterone Progesterone (nm) 0 day Error bar: Standard deviation Microfluidic platform recapitulates human 28-day menstrual cycle hormone control. Xiao et al, Nat Commu, 2017
17 Is the Female Reproductive Chip Possible? Fallopian tube Ovary Uterus Follicle Cervix Vagina Female reproductive tract (FRT) Hormones drive 28 days menstrual cycle Female reproductive organs are connected with each other to support reproductive functions: 28-day menstrual cycle and pregnancy.
18 Ex vivo Female Reproductive Tract in Microfluidic system Ovary Fallopian tube Uterus Uterine Cervix Follicle Ovary Fallopian Fallopian tube Uterus Uterine cervix Liver Integrating and inter-connect multiple female reproductive tissues in vitro based on the microfluidic platform. Xiao et al, Nat Commu, 2017
19 Integrated Female Reproductive Organs-EVATAR Fallopian tube Uterine endometrium Uterine cervix Ovary/follicles Liver Universal spheroids culture media Xiao et al, Nat Commu, 2017
20 Fallopian Tube Tissue Remains Viable in MPS day 0 Epithelium H&E day 0 Cilia beating day 0 OVGP1 High E2 day 7 day 7 day 7 Low E2 OVGP1: oviductal glycoprotein Microfluidic culture supported human fallopian tube viability, and cilia beating is controlled by dynamic hormone secretion patterns. Xiao et al, Nat Commu, 2017
21 Recellularized Endometrium Remain viable in MPS Control Endo-H&E Decelled Endo-H&E Recelled Endo-H&E Recelled Endo-Ki67 Recelled Endo-ER Recelled Endo-PR Endo: uterine endometrium ER: estrogen receptor PR: progesterone receptor Decelled: decellularization Recelled: recellularization Decellularization: removing cells from tissue but keeping extracellular matrix (ECM) scaffold; Recellulzrization: Reseeding cells into the decellularized scaffold. Xiao et al, Nat Commu, 2017 Olalekanet & Kim, BOR, 2017
22 Microfluidic Culture Supports Ectocervix in Response to Hormone from Upstream Ovary Chip day -7 day 0 with E2 peak H&E Ki67 Xiao et al, Nat Commu, 2017 K McKinnon & S Getsios, unpublished
23 Microfluidic Culture Supports Liver Microtissues Microfluidic culture supported human liver spheroid viability and albumin production throughout 28-day culture period.
24 Hormone Secretion in Integrated Microfluidic Culture Ovary only in MPS Multiple tissues in MPS Estradiol (pg / ml) Estrodiol Progesterone Progesterone (ng / ml) Estradiol (pg / ml) Estradiol Progesterone Progesterone (ng / ml) Downstream tissues consumed ovarian secreted hormones and/or integrated tissues changed ovarian hormone expression patterns!
25 Outline I. Using microfluidic system to establish the ex-vivo 28-day menstrual cycle hormone control and female reproductive tract on a chip - Establish II. Screening of female reproductive toxic chemicals - Discover
26 Ovarian Toxicity of Doxorubicin (DOX) Control Estradiol Progesterone DOX 200 nm Estradiol (pg/ml) day * Control 200 nm DOX Progesterone (ng/ml) day Control 200 nm DOX * *p<0.05 DOX is widely used for chemotherapy; WHO recommended DOX significantly changed the follicle ovarian steroid hormone expression patterns in the microfluidic cultures. Xiao et al, Unpublished, 2017
27 Ovarian Toxicity of Doxorubicin Diameter (µm) day Follicle growth 0 nm 2 nm 20 nm 200 nm * Estradiol (pg/ml) day Estradiol secretion 0 nm 2 nm 20 nm 200 nm * Survival rate (%) day Follicle survival 0 nm 2 nm 20 nm 200 nm * Survival rate (%) LC50 of DOX y = x nm DOX dose-dependently inhibited follicle growth, survival, and hormone secretion, DOX has LC50 at nm, which is relevant or even lower than the human exposure levels. Xiao et al, Tox Sci, 2017
28 Ovarian Toxicity of Doxorubicin MII percentage (%) DOX (nm) Oocyte MII percentage * * * * Spindle & chromosome abnormality (%) DOX (nm) Spindle and chromosome morphology * * DOX at low level exposure of 20 nm didn t significantly affect follicle growth and survival, However, DOX significantly increased the percentage of oocytes with abnormal spindle morphology and chromosome misalignment. Xiao et al, Tox Sci, 2017
29 Take Home Message Fallopian tube Ovary Cervix Follicles Uterus Vagina Ovary chip and female reproductive tract chip Introducing gender/sex to co-cultured cells/tissues; DOX has dose-dependent ovarian toxicity, which increases the risk of infertility during chemotherapy; Low but human-relevant exposure level of DOX disrupts oocyte meiotic maturation(avoid this window for oocyte cryopreservation and donation, and pregnancy).
30 Reference 1. Xiao, S., Zhang, J, Liu, M., Iwahata, H., Rogers, HB, Woodruff, TK. (2017) Doxorubicin has dose-dependent toxicity on mouse ovarian follicle development, hormone secretion, and oocyte maturation. Toxicological Sciences. Doi: /toxsci/kfx Xiao, S., Coppeta, J., Rogers, H., Woodruff, TK. (2017) A microfluidic culture model of the human reproductive tract and 28- day menstrual cycle. Nature Communications. Nature Communications. Mar 28; 8: Laronda, MM., Rutz, AL., Xiao, S., Whelan, KA., Duncan, FD., Roth, EW., Woodruff, TK., Shah, R. (2017) A bioprosthetic ovary created using 3D printed microporous scaffolds restores ovarian function in sterilized mice. Nature Communications. May 16; 8: Xiao, S., Zhang, J., Romero MM., Smith KN., Shea, LD., Woodruff, TK. (2015) In vitro follicle growth supports human oocyte meiotic maturation. Scientific Reports. 5: Xiao, S., Duncan, FE., Bai, L., Nguyen, CT., Shea, LD., Woodruff, TK. (2015) Size-specific follicle selection improves mouse oocyte reproductive outcomes. Reproduction: Piirep Olalekan, SA., Burdette, JE., Getsios, S., Woodruff, TK., Kim, JJ. Development of a novel human recellularized endometrium that responds to a 28-day hormone treatment. Biology of Reproduction. 95(5):
31 Acknowledgements University of South Carolina Xiao Lab Yingzheng Wang, MS Mingjun Liu, MS Megan F. Kopp Draper Labs Brett Isenberg, PhD Jeff Borenstein, PhD Jonathan Coppeta, PhD Northwestern University Kim Lab Susan Olalekan, PhD Sevim Yildiz Arslan, PhD J. Julie Kim, PhD Northwestern University Teresa Woodruff Lab Hunter Rogers, MS Jiyang Zhang, MS Mingjun Liu, BS Monica Laronda, PhD Kelly McKinnon Peter Chen, MS Mingyang Jiang, MS Lu Bai, MS Cat Nugyen, MS Alex Rashedi, BS Jie Zhu, MD Danijela Dokic, MD Beth Sefton, PhD Chanel Arnold-Murray Financial supports: NIEHS/NCATS: UH3TR Subcontract award to S Xiao (UH3TR001207) Arnold School of Public Health Research Fund
Oncofertility: Fertility Preservation and Restoration Progress Toward an Ovarian Bioprosthesis
Oncofertility: Fertility Preservation and Restoration Progress Toward an Ovarian Bioprosthesis Fertility Needs in Pediatric Cancer Patients Acute Lymphoblastic Leukemia Cells in ovarian cortical tissue
More informationFemale Reproductive System. Lesson 10
Female Reproductive System Lesson 10 Learning Goals 1. What are the five hormones involved in the female reproductive system? 2. Understand the four phases of the menstrual cycle. Human Reproductive System
More informationReviewer #1 (Remarks to the Author)
Reviewer #1 (Remarks to the Author) This manuscript presents an engineering system designed to culture and maintain living tissue from female reproductive organs. The modular capacity, reconfigurability,
More information9.4 Regulating the Reproductive System
9.4 Regulating the Reproductive System The Reproductive System to unite a single reproductive cell from a female with a single reproductive cell from a male Both male and female reproductive systems include
More informationA microfluidic culture model of the human reproductive tract and 28-day menstrual cycle
Received 3 Aug 1 Accepted 13 Jan 17 Published Mar 17 DOI: 1.13/ncomms15 OPEN A microfluidic culture model of the human reproductive tract and -day menstrual cycle Shuo Xiao 1, *, Jonathan R. Coppeta, *,
More informationInvestigation: The Human Menstrual Cycle Research Question: How do hormones control the menstrual cycle?
Investigation: The Human Menstrual Cycle Research Question: How do hormones control the menstrual cycle? Introduction: The menstrual cycle (changes within the uterus) is an approximately 28-day cycle that
More information10.7 The Reproductive Hormones
10.7 The Reproductive Hormones December 10, 2013. Website survey?? QUESTION: Who is more complicated: men or women? The Female Reproductive System ovaries: produce gametes (eggs) produce estrogen (steroid
More informationThe Human Menstrual Cycle
The Human Menstrual Cycle Name: The female human s menstrual cycle is broken into two phases: the Follicular Phase and the Luteal Phase. These two phases are separated by an event called ovulation. (1)
More informationFemale Reproductive Physiology. Dr Raelia Lew CREI, FRANZCOG, PhD, MMed, MBBS Fertility Specialist, Melbourne IVF
Female Reproductive Physiology Dr Raelia Lew CREI, FRANZCOG, PhD, MMed, MBBS Fertility Specialist, Melbourne IVF REFERENCE Lew, R, Natural History of ovarian function including assessment of ovarian reserve
More informationReproduction and Development. Female Reproductive System
Reproduction and Development Female Reproductive System Outcomes 5. Identify the structures in the human female reproductive system and describe their functions. Ovaries, Fallopian tubes, Uterus, Endometrium,
More informationChapter 14 Reproduction Review Assignment
Date: Mark: _/45 Chapter 14 Reproduction Review Assignment Multiple Choice Identify the choice that best completes the statement or answers the question. 1. Use the diagram above to answer the next question.
More information1. During the follicular phase of the ovarian cycle, the hypothalamus releases GnRH.
1. During the follicular phase of the ovarian cycle, the hypothalamus releases GnRH. 2. This causes the anterior pituitary to secrete small quantities of FSH and LH. 3. At this time, the follicles in the
More informationHormonal Control of Human Reproduction
Hormonal Control of Human Reproduction Bởi: OpenStaxCollege The human male and female reproductive cycles are controlled by the interaction of hormones from the hypothalamus and anterior pituitary with
More informationSample Provincial exam Q s: Reproduction
Sample Provincial exam Q s: Reproduction 11. Functions Testosterone Makes the male sex organs function normally, and also inhibits hypothalamus s release of GnRH and thus LH & FSH and thus testosterone
More informationReproductive Hormones
Reproductive Hormones Male gonads: testes produce male sex cells! sperm Female gonads: ovaries produce female sex cells! ovum The union of male and female sex cells during fertilization produces a zygote
More informationWeb Activity: Simulation Structures of the Female Reproductive System
differentiate. The epididymis is a coiled tube found along the outer edge of the testis where the sperm mature. 3. Testosterone is a male sex hormone produced in the interstitial cells of the testes. It
More informationN. Shirazian, MD. Endocrinologist
N. Shirazian, MD Internist, Endocrinologist Inside the ovary Day 15-28: empty pyfollicle turns into corpus luteum (yellow body) Immature eggs Day 1-13: 13: egg developing inside the growing follicle Day
More informationREPRODUCCIÓN. La idea fija. Copyright 2004 Pearson Education, Inc., publishing as Benjamin Cummings
REPRODUCCIÓN La idea fija How male and female reproductive systems differentiate The reproductive organs and how they work How gametes are produced and fertilized Pregnancy, stages of development, birth
More informationIs it the seed or the soil? Arthur Leader, MD, FRCSC
The Physiological Limits of Ovarian Stimulation Is it the seed or the soil? Arthur Leader, MD, FRCSC Objectives 1. To consider how ovarian stimulation protocols work in IVF 2. To review the key events
More informationChapter 46 ~ Animal Reproduction
Chapter 46 ~ Animal Reproduction Overview Asexual (one parent) fission (parent separation) budding (corals) fragmentation & regeneration (inverts) parthenogenesis Sexual (fusion of haploid gametes) gametes
More informationNeil Goodman, MD, FACE
Initial Workup of Infertile Couple: Female Neil Goodman, MD, FACE Professor of Medicine Voluntary Faculty University of Miami Miller School of Medicine Scope of Infertility in the United States Affects
More informationOutline. Male Reproductive System Testes and Sperm Hormonal Regulation
Outline Male Reproductive System Testes and Sperm Hormonal Regulation Female Reproductive System Genital Tract Hormonal Levels Uterine Cycle Fertilization and Pregnancy Control of Reproduction Infertility
More informationMULTIPLE CHOICE: match the term(s) or description with the appropriate letter of the structure.
Chapter 27 Exam Due NLT Thursday, July 31, 2015 Name MULTIPLE CHOICE: match the term(s) or description with the appropriate letter of the structure. Figure 27.1 Using Figure 27.1, match the following:
More informationBio 322 Human Anatomy Objectives for the laboratory exercise Female Reproductive System
Bio 322 Human Anatomy Objectives for the laboratory exercise Female Reproductive System Required reading before beginning this lab: Saladin, KS: Human Anatomy 5 th ed (2017) Chapter 26 For this lab you
More informationPhases of the Ovarian Cycle
OVARIAN CYCLE An ovary contains many follicles, and each one contains an immature egg called an oocyte. A female is born with as many as 2 million follicles, but the number is reduced to 300,000 to 400,000
More informationEndocrinology of the Female Reproductive Axis
Endocrinology of the Female Reproductive Axis girlontheriver.com Geralyn Lambert-Messerlian, PhD, FACB Professor Women and Infants Hospital Alpert Medical School at Brown University Women & Infants BROWN
More informationSISTEMA REPRODUCTOR (LA IDEA FIJA) Copyright 2004 Pearson Education, Inc., publishing as Benjamin Cummings
SISTEMA REPRODUCTOR (LA IDEA FIJA) How male and female reproductive systems differentiate The reproductive organs and how they work How gametes are produced and fertilized Pregnancy, stages of development,
More informationReproductive System. Testes. Accessory reproductive organs. gametogenesis hormones. Reproductive tract & Glands
Reproductive System Testes gametogenesis hormones Accessory reproductive organs Reproductive tract & Glands transport gametes provide nourishment for gametes Hormonal regulation in men Hypothalamus - puberty
More informationChapter 27 The Reproductive System. MDufilho
Chapter 27 The Reproductive System 1 Figure 27.19 Events of oogenesis. Before birth Meiotic events 2n Oogonium (stem cell) Mitosis Follicle development in ovary Follicle cells Oocyte 2n Primary oocyte
More informationThe reproductive lifespan
The reproductive lifespan Reproductive potential Ovarian cycles Pregnancy Lactation Male Female Puberty Menopause Age Menstruation is an external indicator of ovarian events controlled by the hypothalamicpituitary
More informationXIAO, SHUO CV 1 SHUO XIAO
XIAO, SHUO CV 1 EDUCATION SHUO XIAO Department. Environmental Health Sciences Arnold School of Public Health, University of South Carolina Discovery I building, 915 Greene St, Rm 327, Columbia, SC, 29208
More informationFemale Reproductive System. Justin D. Vidal
Female Reproductive System Justin D. Vidal If you cannot identify the tissue, then it is probably part of the female reproductive system! Introduction The female reproductive system is constantly changing,
More informationREPRODUCTION & GENETICS. Hormones
REPRODUCTION & GENETICS Hormones http://www.youtube.com/watch?v=np0wfu_mgzo Objectives 2 Define what hormones are; Compare and contrast the male and female hormones; Explain what each hormone in the mail
More informationMULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question.
MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 1) Which of the following hormones controls the release of anterior pituitary gonadotropins? A) LH
More informationHORMONES & REPRODUCTION OUTLINE
1 HORMONES & REPRODUCTION Dr. Steinmetz OUTLINE 2 The Endocrine System Sexual Reproduction Hormonal Role in Sexual Differentiation Gender Differences and Gender Identity Characterizing Complex Behaviors
More informationEndocrine System Hormones & Homeostasis. Regents Biology
Endocrine System Hormones & Homeostasis 2009-2010 Homeostasis Homeostasis maintaining internal balance in the body organism must keep internal conditions stable even if environment changes also called
More informationChapter 28: REPRODUCTIVE SYSTEM: MALE
Chapter 28: REPRODUCTIVE SYSTEM: MALE I. FUNCTIONAL ANATOMY (Fig. 28.1) A. Testes: glands which produce male gametes, as well as glands producing testosterone 2. Seminiferous tubules (Fig.28.3; 28.5) a.
More informationTestes (male gonads) -Produce sperm -Produce sex hormones -Found in a sac called the scrotum -Suspended outside of the body cavity for temperature
REPRODUCTION Testes (male gonads) -Produce sperm -Produce sex hormones -Found in a sac called the scrotum -Suspended outside of the body cavity for temperature reduction -Testes wall made of fibrous connective
More informationSTRUCTURE AND FUNCTION OF THE FEMALE REPRODUCTIVE SYSTEM
Unit 7B STRUCTURE AND FUNCTION OF THE FEMALE REPRODUCTIVE SYSTEM LEARNING OBJECTIVES 1. Learn the structures of the female reproductive tract. 2. Learn the functions of the female reproductive tract. 3.
More informationChapter 14 The Reproductive System
Biology 12 Name: Reproductive System Per: Date: Chapter 14 The Reproductive System Complete using BC Biology 12, page 436-467 14. 1 Male Reproductive System pages 440-443 1. Distinguish between gametes
More informationStudy Guide Answer Key Reproductive System
Biology 12 Human Biology Textbook: BC Biology 12 Study Guide Answer Key Reproductive System 1. Distinguish between a gamete and a gonad using specific examples from the male and female systems. Gonads
More informationI. ART PROCEDURES. A. In Vitro Fertilization (IVF)
DFW Fertility Associates ASSISTED REPRODUCTIVE TECHNOLOGY (ART) Welcome to DFW Fertility Associates/ Presbyterian-Harris Methodist Hospital ARTS program. This document provides an overview of treatment
More informationThe beginning of puberty is marked by the progressive increase in the production of sex hormones.
Puberty is characterized by the changes that prepare the human body for the ability to reproduce. This stage generally occurs between the ages of 10 and 14 years old. The beginning of puberty is marked
More informationThe Male Reproductive System
The Male Reproductive System Male Reproductive System The male sex cell is a sperm cell The whole purpose is to produce and deliver sperm to the egg Structure of a Human Sperm Cell Streamlined, built to
More informationCASE 41. What is the pathophysiologic cause of her amenorrhea? Which cells in the ovary secrete estrogen?
CASE 41 A 19-year-old woman presents to her gynecologist with complaints of not having had a period for 6 months. She reports having normal periods since menarche at age 12. She denies sexual activity,
More informationBiology of fertility control. Higher Human Biology
Biology of fertility control Higher Human Biology Learning Intention Compare fertile periods in females and males What is infertility? Infertility is the inability of a sexually active, non-contracepting
More informationWhat are the main functions of the male reproductive system? 1. Produce sperm 2. Deposit sperm into the female 3. Provide a pathway for the removal
What are the main functions of the male reproductive system? 1. Produce sperm 2. Deposit sperm into the female 3. Provide a pathway for the removal of urine Where is sperm produced? -In the 2 testes What
More informationFemale reproductive cycle: A Comprehensive Review Rachel Ledden Paper for Bachelors in Science January 20, 2018
Running head: 1 Female reproductive cycle: A Comprehensive Review Rachel Ledden Paper for Bachelors in Science January 20, 2018 Female reproductive cycle: A Comprehensive Review 2 The reproductive cycle
More informationMohammad. Renad zakaria ---
13 Mohammad Renad zakaria --- Before we start: - I didn t follow the record order, for organizing purposes. - I added extra information from our text box which is Guyton 12 th edition, pages 987-997, actually
More informationEmbryology Lecture # 4
1 Quick Review: Oogenesis : - Oogonia start appear in the ovary when the age of the fetus 1 is th (5 week). - Then the Oogonia transformed into 1ry Oocyte. - 1ry Oocyte is surrounded by a follicle (cover).
More informationMale Reproduction Organs. 1. Testes 2. Epididymis 3. Vas deferens 4. Urethra 5. Penis 6. Prostate 7. Seminal vesicles 8. Bulbourethral glands
Outline Terminology Human Reproduction Biol 105 Lecture Packet 21 Chapter 17 I. Male Reproduction A. Reproductive organs B. Sperm development II. Female Reproduction A. Reproductive organs B. Egg development
More information2 - male hormones/ female system
2 - male hormones/ female system May 7, 2012 5:08 PM I) Testosterone: main androgen (male sex hormone) A) Intro to Testosterone: -steroid hormone produced by interstitial cells in testes -produced in response
More informationStage 4 - Ovarian Cancer Symptoms
WELCOME Stage 4 - Ovarian Cancer Symptoms University of Baghdad College of Nursing Department of Basic Medical Sciences Overview of Anatomy and Physioloy II Second Year Students Asaad Ismail Ahmad,
More informationAge and Fertility. A Guide for Patients Revised 2012 Copyright 2012 by the American Society for Reproductive Medicine
1 Age and Fertility A Guide for Patients Revised 2012 Copyright 2012 by the American Society for Reproductive Medicine INTRODUCTION Fertility changes with age. Both males and females become fertile in
More informationStudent Academic Learning Services Page 1 of 5 Reproductive System Practice
Student Academic Learning Services Page 1 of 5 Reproductive System Practice Fill in the blanks using the words listed: accessory glands, vas deferens, penis, scrotum, fallopian tube, testes, urethra, vagina,
More informationReproductive physiology. About this Chapter. Case introduction. The brain directs reproduction 2010/6/29. The Male Reproductive System
Section Ⅻ Reproductive physiology Ming-jie Wang E-Mail: mjwang@shmu.edu.cn About this Chapter The reproductive organs and how they work the major endocrine functions of sexual glands actions of sex hormones
More informationFertility Diagnostics
Fertility Diagnostics Fertility hormones measured on PATHFAST For internal use only Diagnostics PATHFAST Chemiluminescence-immuno-analyzer 1 Content: page 1. Fertility hormones - general aspects 1.1 Reproductive
More informationReproductive physiology
Reproductive physiology Sex hormones: Androgens Estrogens Gestagens Learning objectives 86 (also 90) Sex Genetic sex Gonadal sex Phenotypic sex XY - XX chromosomes testes - ovaries external features Tha
More informationLUTEAL PHASE SUPPORT. Doç. Dr. Nafiye Yılmaz. Zekai Tahir Burak Kadın Sağlığı Eğitim Araştırma Hastanesi
LUTEAL PHASE SUPPORT Doç. Dr. Nafiye Yılmaz Zekai Tahir Burak Kadın Sağlığı Eğitim Araştırma Hastanesi TAJEV, 2014 1 ART & success *Live birth rate 2 Optimal luteal phase Etiology of luteal phase deficiency
More informationBio 12- Ch. 21: Reproductive System
Bio 12- Ch. 21: Reproductive System 21.1- Male Reproductive System o Male anatomy o Testes and how they relate to sperm production and male sex hormones o Hormone regulation in males 21.2- Female Reproductive
More informationHuman Physiology 6.6- Hormones, Homeostasis, and Reproduction
Human Physiology 6.6- Hormones, Homeostasis, and Reproduction Essential idea: Hormones are used when signals need to be widely distributed. Application: William Harvey s investigation of sexual reproduction
More informationFunctions of male Reproductive System: produce gametes deliver gametes protect and support gametes
Functions of male Reproductive System: produce gametes deliver gametes protect and support gametes Spermatogenesis occurs in the testes after puberty. From the testes they are deposited into the epididymas
More informationChapter 36 Active Reading Guide Reproduction and Development
Name: AP Biology Mr. Croft Chapter 36 Active Reading Guide Reproduction and Development Section 1 1. Distinguish between sexual reproduction and asexual reproduction. 2. Which form of reproduction: a.
More informationUnit 15 ~ Learning Guide
Unit 15 ~ Learning Guide Name: INSTRUCTIONS Complete the following notes and questions as you work through the related lessons. You are required to have this package completed BEFORE you write your unit
More informationa. the tail disappears b. they become spermatids c. they undergo capacitation d. they have been stored in the uterus for several days
(2 points each) Multiple Choice. Read each question thoroughly before answering. From the choices available, choose the answer that is the most correct. Place all answers on the accompanying answer sheet.
More informationI. Endocrine System & Hormones Figure 1: Human Endocrine System
I. Endocrine System & Hormones Figure 1: Human Endocrine System Endocrine System: a) Endocrine glands are ductless since they lack specific vessels for the transport of hormones throughout the body. Instead,
More informationSperm production. Sperm production. Meiosis. Mitosis. The cells of Leydig in testes secrete
Sperm production Ductus deferens Epididymis The cells of Leydig in testes secrete Seminiferous testosterone (T) tubules T secreted at puberty produces 2 o sex characteristics, spermatogenesis, & maintain
More informationSperm production. Sperm production. Controlling sperm production. Meiosis. Mitosis. The cells of Leydig in testes secrete
Ductus deferens Sperm production Epididymis The cells of Leydig in testes secrete Seminiferous testosterone (T) tubules T secreted at puberty produces 2 o sex characteristics, spermatogenesis, & maintain
More informationOVARY The surface of the ovary is covered with surface epithelium
OVARY Cow The ovary, or female gonad, is: 1. an exocrine gland, producing oocytes 2. an endocrine gland, secreting hormones, i.e., estrogen and progesterone OVARY OVARY The surface of the ovary is covered
More informationHuman Reproductive System
Human Reproductive System I. The male reproductive anatomy is a delivery system for sperm. A. The male s external reproductive organs consist of the scrotum and penis. 1. The penis is the external organ
More informationReproductive System (Hormone Function) Physiology Department Medical School, University of Sumatera Utara
Reproductive System (Hormone Function) Physiology Department Medical School, University of Sumatera Utara 1 Endocrine Control: Three Levels of Integration Hormones of the hypothalamic-anterior pituitary
More informationHuman Reproductive Anatomy The female anatomy first just the reproductive parts:
Human Reproduction: Structures, Functions, and Hormones Human reproduction differs from the mechanisms in lower vertebrates and many invertebrates. We depend on internal fertilization sperm are deposited
More informationHuman Reproductive System
Human Reproductive System I. The male reproductive anatomy is a delivery system for sperm. A. The male=s external reproductive organs consist of the scrotum and penis. 1. The penis is the external organ
More information6.6 Hormones, homeostasis and reproduction
6.6 Hormones, homeostasis and reproduction Essential idea: Hormones are used when signals need to be widely distributed. Thyroxin is a hormone produced by the thyroid gland. It's key role is in controlling
More informationPuberty and Fertility. Normal Female Puberty PUBERTY! What about girls with Galactosemia? E Puberty and Fertility Badik Spencer 1
Puberty and Fertility Jennifer Badik*, MD Pediatric Endocrinology and Jessica Spencer*, MD, MSc Reproductive Endocrinology and Infertility Part One PUBERTY! *no conflicts of interest to report Every girl
More informationInfertility treatment
In the name of God Infertility treatment Treatment options The optimal treatment is one that provide an acceptable success rate, has minimal risk and is costeffective. The treatment options are: 1- Ovulation
More informationLOW RESPONDERS. Poor Ovarian Response, Por
LOW RESPONDERS Poor Ovarian Response, Por Patients with a low number of retrieved oocytes despite adequate ovarian stimulation during fertility treatment. Diagnosis Female About Low responders In patients
More informationAssisted Reproduction. By Dr. Afraa Mahjoob Al-Naddawi
Assisted Reproduction By Dr. Afraa Mahjoob Al-Naddawi Learning Objectives: By the end of this lecture, you will be able to: 1) Define assisted reproductive techniques (ART). 2) List indications for various
More informationto ensure the. Sexual reproduction requires the (from the mother) by a (from the father). Fertilization is the fusion of.
The Reproductive System Fill-In Notes Purpose of life: to ensure the. Stages of Human Development Sexual reproduction requires the (from the mother) by a (from the father). Fertilization is the fusion
More informationThe Science of your Cycle
The Science of your Cycle Day 3: Get to know your cycle (Part I) with Jennifer Aldoretta Cofounder & CEO of Groove Today s goals Learn how your hormones work together to create the changes that happen
More informationReproduction Worksheet
Name: Date: Reproduction Worksheet Directions: Base your answers to questions 1-4 on the diagram below and your knowledge of biology. 1. Identify the structure in which sperm is produced. What is the name
More informationEndometrial Preparation for Frozen Embryo Transfer (FET) Zitao Liu, MD, PhD New Hope Fertility Center, NY
Endometrial Preparation for Frozen Embryo Transfer (FET) Zitao Liu, MD, PhD New Hope Fertility Center, NY Natural Cycle FET Protocol for endometrial preparation N FET, including modified N FET HRT FET:
More informationTop 5 Fertility Secrets Revealed
Top 5 Fertility Secrets Revealed by Melinda Stevens Brought to you by http://www.pregnancy-online.info All rights reserved, 2007. Copyright 2007, Pregnancy-Online.info 2 Hi, thanks for downloading my free
More informationUrinary System Chapter 16
Urinary System Chapter 16 1 Urology- the branch of medicine that treats male and female urinary systems as well as the male reproductive system. Nephrology- the scientific study of the anatomy, physiology,
More informationReproductive Endocrinology. Isabel Hwang Department of Physiology Faculty of Medicine University of Hong Kong Hong Kong May2007
Reproductive Endocrinology Isabel Hwang Department of Physiology Faculty of Medicine University of Hong Kong Hong Kong May2007 isabelss@hkucc.hku.hk A 3-hormone chain of command controls reproduction with
More informationBiology of gender Sex chromosomes determine gonadal sex (testis-determining factor)
Indifferent ducts of embryo Biology of gender Sex chromosomes determine gonadal sex (testis-determining factor) Y chromosome present Y chromosome absent Phenotypic sex is depends on development of external
More informationBiology of gender Sex chromosomes determine gonadal sex (testis-determining factor)
Indifferent ducts of embryo Y chromosome present Y chromosome absent Male Female penis ovary uterus vagina testis Biology of gender Sex chromosomes determine gonadal sex (testis-determining factor) Phenotypic
More informationBiology of Reproduction- Zool 346 Exam 2
Biology of Reproduction- Zool 346 Exam 2 ANSWER ALL THE QUESTIONS ON THE ANSWER SHEET. THE ANSWER ON THE ANSWER SHEET IS YOUR OFFICIAL ANSWER. Some critical words are boldfaced. This exam is 7 pages long.
More informationUse of in vitro maturation for fertility preservation
Use of in vitro maturation for fertility preservation G. Arroyo Servei de Medicina de la Reproducció Departament d Obstetrícia, Ginecologia i Reproducció INSTITUT UNIVERSITARI DEXEUS MEDICAL STRATEGY TO
More informationFirst you must understand what is needed for becoming pregnant?
What is infertility? Infertility means difficulty in becoming pregnant without using contraception. First you must understand what is needed for becoming pregnant? Ovum from the woman to combine with a
More informationEgg donation in an in vitro fertilization program: an alternative approach to cycle synchronization and timing of embryo transfer
FERTILITY AND STERILITY Copyright tl 1989 The American Fertility Society Printed on acid-free paper in U. S.A. Egg donation in an in vitro fertilization program: an alternative approach to cycle synchronization
More informationCauses of Infertility and Treatment Options
Causes of Infertility and Treatment Options Dr Mrs.Kiran D. Sekhar Former vice President-FOGSI Former Chairperson- Genetics and Foetal medicine-fogsi Founder and Medical Director-Kiran Infertility centre
More informationDATE: NAME: CLASS: Chapter 14 Test
Multiple Choice Questions Decide which of the choices best completes the statement or answers the question. Locate that question number on the separate answer sheet provided. Use the procedure described
More informationHold On To Your Dreams
Hold On To Your Dreams Dr. Michael Kettel Dr. Sandy Chuan 1. THE BASICS OF IVF & EMBRYO DEVELOPMENT 2. IVF ADD-ONS - MYTH VS. SCIENCE IN VITRO FERTILIZATION 1. Ovarian Stimulation 2. Egg Retrieval 3. Create
More informationEmbryo Selection after IVF
Embryo Selection after IVF Embryo Selection after IVF Many of human embryos produced after in vitro fertilization carry abnormal chromosomes. Placing a chromosomally normal embryo (s) into a normal uterus
More informationReproductive Endocrinology & Infertility Glossary
Reproductive Endocrinology & Infertility Glossary The following is a glossary of terms you may hear during your association with the University of Mississippi Health Care's reproductive endocrinology and
More informationLecture 14: Conception, Fertility, Early Fetal Loss. provera. Depo-provera. Early Fetal Loss. Implanon. Norplant. Nuva Ring.
Lecture 14: Conception, Fertility, Early Fetal Loss Birth Control (cont.) Conception What Influences Probability of Conception? Early Fetal Loss Infertility Fertility Enhancement Depo-provera provera Injectable
More informationEndocrine and Reproductive Systems. Chapter 39: Biology II
Endocrine and Reproductive Systems Chapter 39: Biology II The Endocrine System Made up of glands that release their products into the bloodstream These products broadcast messages throughout the body Chemicals
More informationReproductive Health and Pituitary Disease
Reproductive Health and Pituitary Disease Janet F. McLaren, MD Assistant Professor Division of Reproductive Endocrinology and Infertility Department of Obstetrics and Gynecology jmclaren@uabmc.edu Objectives
More informationPhysiology of Male Reproductive System
Physiology of Male Reproductive System the anterior pituitary gland serves as the primary control of reproductive function at puberty Ant Pituitary secretes FSH & large amounts of LH (ICSH) FSH & LH cause
More information