HIMANSHU SHARMA. POSITION APPLIED FOR Postdoctoral Fellow
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1 HIMANSHU SHARMA POSITION APPLIED FOR Postdoctoral Fellow Research Interests: Biochemistry and molecular cell biology Molecular Genetic Quality control and trafficking mechanism of misfolded proteins Therapeutic management of misfolded proteins Thrust area in PhD research work Elucidate the molecular genetics of CFTR gene in male infertility and functional characterization of rare Indian CFTR gene mutations. EDUCATIONAL BACKGROUND Ph. D Biochemistry (Thesis submitted) INSTITUTE PGIMER, Chandigarh YEARS ATTENDED M.Sc. Biochemistry Class/Division First (70.08%) UNIVERSITY Aligarh Muslim University, Aligarh, India YEARS ATTENDED B.Sc. (Hon.) Biochemistry Class/Division First (75.33%) UNIVERSITY Aligarh Muslim University, Aligarh, India YEARS ATTENDED Sen. Sec. School Certificate Class/Division First (Distinction) (66 %) UNIVERSITY Aligarh Muslim University, Aligarh, India YEARS ATTENDED Sec. School Certificate Class/Division First (Distinction) (75 %) SCHOOL Our lady of Fatima Hr. Sec School, Aligarh, India YEARS ATTENDED
2 RESEARCH EXPERIENCE Six month training (June December 2012) in university of Poitiers France, work on short term project entitled Characterization of Novel mutation found in Classical and Infertile CF male of Indian population: A molecular approach to establish genotype and phenotype correlation. From April 2012 onward working as senior research fellow in project entitled Molecular Basis of Male Infertility: Diagnostic and Genetic Implications. Worked as research assistant in an ICMR funded project Nov Mar 2012 in PGIMER Chandigarh Identification and molecular characterization of CFTR gene mutations and microdeletion in human Y chromosome AZF candidate gene in male infertility: Diagnostic and genetic implications. One year M. Sc dissertation work on Effect of fish oil on cisplatin induced hepatotoxicity. RESEARCH PUBLICATIONS H Sharma, R.S. Mavuduru, S.K. Singh, R. Prasad. Heterogeneous spectrum of mutations in CFTR gene from Indian patients with congenital absence of vas deferens and their association with cystic fibrosis genetic modifiers. Mol Hum Rep. 2014; 20: PMID: H Sharma, M Jollivet Souchet, Callebaut I, R Prasad, F Becq. Function, Pharmacological correction and maturation of new Indian CFTR gene mutations. J of cystic Fibrosis. 2014, doi: /j.jcf PMID: H Sharma, R.S. Mavuduru, S.K. Singh, R. Prasad. Increased frequency of CFTR gene mutation in Indian patients with non CBAVD obstructive azoospermia and spermatogenic failure. Gene. 2014; 10:548: PMID:
3 R Prasad, H Sharma, G Kaur. Molecular basis of cystic fibrosis disease an Indian perspective. Indian J of Clin Biochem: 2010; 25(4): Citations; 13 H Sharma. Hepatoprotective effect of fish oil against cisplatin induced oxidative stress in male rats. Int. J of Inv. Res. 2012; 1(1): H Sharma, R.S. Mavuduru, S.K. Singh, R. Prasad. Frequency of Y chromosome microdeletion in Indian infertile males with spermatogenic failure. (Communicated in Andrologia) Publications in Books and proceedings N Sharma, H Sharma, U Sharma, R Prasad. Biochemistry, Molecular Biology and Genetics of Cystic Fibrosis. Chapter in book Biochemistry, Molecular biology and Genetics of Genetic Disorders. Research signpost publication group. ISBN No H Sharma Declining male infertility: An alarming trend A short informative article for general awareness accepted for published in Scientific Malaysia an international magazine. Issue 6: august H Sharma, Ravimohan S M, S K Singh, Prasad R. Role of CFTR in regulating spermatogenesis and Implications in male infertility. Abstract published 63 rd in annual proceedings of American society of Human Genetics H Sharma, M Jollivet Souchet, Callebaut I, R Prasad, F Becq. Characterization of Novel mutations found in Classical and Infertile CF males of Indian population: A molecular approach to establish genotype and phenotype correlation. Abstract published in 63 rd annual proceedings of American society of Human Genetics H Sharma, Ravimohan S M, S K Singh, Prasad R. Molecular Basis of Male Infertility: An Indian Perspective. Abstract published in 62 nd annual proceedings of American society of Human Genetics
4 TRAININGS AND SEMINARS ATTENDED (National & International) Six month training (June December 2012) in university of Poitiers France, worked on short term project entitled Characterization of Novel mutation found in Classical and Infertile CF male of Indian population: A molecular approach to establish genotype and phenotype correlation. Presented poster entitled Role of CFTR in spermatogenesis and implications in male infertility in 63 rd annual meeting of American society of Human Genetics. An international conference in Boston USA held in October Presented poster entitled Identification and Molecular characterization of CFTR gene mutations in Indian infertile CAVD male population and awarded consolation prize in international conference on reproductive health with emphasis on stratagies on family planning and 22 nd annual meeting of Indian society for the study of reproduction and fertility (ISSRF). Feb Presented poster entitled Spectrum of CFTR gene mutation in Indian infertile CBAVD male Population and awarded first prize in CME Molecular basis of genetic disorders Dec 2012 organized by dept of biochemistry PGIMER, Chandigarh on the occasion of golden jubilee celebration of PGIMER, Chandigarh. Participated in oral presentation current scenario of cystic fibrosis disease: An Indian Perspective in 10 th Annual research festival march school of life science JNU, New Delhi. Presented poster entitled functional characterization of rare/novel mutation in Indian CF And infertile male population in the 9 th national research scholar meet in life science-2013, held at advance centre for treatment, research and education in cancer (ACTREC), Navi Mumbai. Participated in the two day work shop (16-17 July 2014) on fluorescence in situ hybridization (FISH) technology organized by Abbott molecular & GMCH, Chandigarh. 4
5 Participated in the symposium Advanced techniques in basic research: tools of 21 st Century held on May, 2010 at PGIMER, Chandigarh. Techniques well acquainted Well experience with all the techniques used in molecular genetics studies like: DNA isolation from blood and tissues RNA isolation, ARMS PCR, RFLP, SSCP, PAGE. Modern techniques used in functional characterization and in-vitro assay like: mammalian Cell culture, western blot analysis (CFTR proteins), immunoflouresence, radioactive iodide efflux assay (basic knowledge), site directed mutagenesis and expression of plasmid in mammalian cells. Basic knowledge and hand on practice over biochemical assay techniques like HPLC and GC. Well expertise to use various bioinformatics tools like PolyPhen-2, mmb, HOPE, isatble etc., implicated in functional prediction of human SNPs. As well as basic idea and hand on practice in techniques of homology modelling. FELLOWSHIPS, AWARDS AND PROFESSIONAL AFFILIATIONS Fellowship- Awarded prestigious Indo-French Charpak PhD fellowship-2012 by French government for six month training in university of Poitiers France, where I have carried out part of my PhD work in collaboration with French laboratory 17 June December Awards- Awarded ICMR senior research fellowship in Qualified national GATE examination 2010 with 94.8 percentile. Awarded first prize for poster presentation in CME Molecular basis of Genetic disorders December 2012 organized by department of biochemistry on the occasion of golden jubilee celebration of PGIMER Chandigarh. 5
6 Awarded consolation prize for poster presentation in International conference on Reproductive Health and Emphasis on Strategy of Family Planning Organized by ICMR and ISSRF. Feb Membership- Life time member of Indian society for study of reproduction and fertility (ISSRF) (I.D No. 1026). Life time member of Indian society of human genetics. Member of American society of Human genetics. (I.D No ). PERSONAL DATA Date of Birth : 30/04/85 Gender : Male Nationality : Indian CONTACT INFORMATION Address (Present) Address (Permanent) Mobile No. : E Mail Address : Lab No. 339 Dept of biochemistry Research block-a sector-12 PGIMER, Chandigarh. : M-Shatabdi Nagar Aligarh-(UP) : himanshu.bcm@gmail.com Place: Chandigarh Himanshu Sharma REFERENCES 1. Dr. Rajendra Prasad 2. Dr. Ravi Mohan Prof & Head Assistant professor Dept. of Biochemistry, PGIMER Dept of Urology Chandigarh. India PGIMER, Chandigarh fateh1977@gmail.com ravismi2003@yahoo.com 3. Dr. S.K Singh Professor Dept. Of Urology, PGIMER, Chandigarh, India. Sharwanksingh2002@yahoo.com 6
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