Old Truths. Why Ultrasound? Embryo Transfer The Last 20 Years. Embryo Transfer The Last 20 Years

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1 Proceedings, pplied eproductive trategies in Beef Cattle October -6, 0 taunton, V mbryo ransfer he Last 0 Years andall H. Hinshaw, D.V.. shby mbryos 0 Grace Chapel oad Harrisonburg, V randall@ashbyvets.com mbryo ransfer he Last 0 Years Old ruths Donors ecips Chance New echnologies/ideas Ultrasound Donor synchronization ecip synchronization xpectations exed semen IV easons for Costs Old ruths Ultrasound he donor is largest variable in embryo production Not H/protocol Not practitioner Not environment nm n he recip is the largest variable in conception rate Not the embryo Not the practitioner Chance is the largest variable in small groups Why Ultrasound? ble to diagnose pregnancy earlier ore accurate Less invasive Confirm fetal viability Diagnose fetal anomalies etal sexing ssess superovulation before breeding Understand follicular dynamics

2 Proceedings, pplied eproductive trategies in Beef Cattle October -6, 0 taunton, V ynchronization and anagement of ecipients/donors ynchronization and anagement of ecipients/donors 0 ollicle Diam meter (mm) 6 0 Wave Wave Wave POGLNDIN Lutalyse/strumate GONDOOPIN - LING HOON (GnH) - Cystorelin, actrel, ertagyl POGIN elengestrol cetate (G); CID (controlled internal drug release device) Days J. P. Kastelic, 99 ynchronization and anagement of ecipients/donors ynchronization and anagement of ecipients/donors POGLNDIN Lutalyse/strumate Prostaglandin injected between day and 0 of the estrous cycle regresses the corpus luteum (CL). GONDOOPIN - LING HOON (GnH) - Cystorelin, actrel, ertagyl GnH causes a Luteinizing Hormone surge: LH follicle turnover / new follicle development CL ynchronization and anagement of ecipients/donors ynchronization and anagement of ecipients/donors GONDOOPIN - LING HOON (GnH) - Cystorelin, actrel, ertagyl POGIN elengestrol cetate (G); CID (controlled internal drug release device) GnH causes a Luteinizing Hormone surge: LH dministration of a progestin (G or CID) inhibits heat and blocks ovulation = artificial CL follicle turnover / new follicle development ovulation of a follicle

3 Proceedings, pplied eproductive trategies in Beef Cattle October -6, 0 taunton, V Donor anagement - iming of H for uperovulation ollicle Diam meter (mm) 0 6 Get a tart Wait 7- days H Wave Wave Wave Days J. P. Kastelic, 99 Donor anagement - H chedule for uperovulation ID COLLCION oday Donor (Day ) Donor (Day ) Donor (Day ) H Injections I Collection Day I Donor anagement H chedule for uperovulation ID COLLCION CID GnH 0 ameter (mm) ollicle Dia 6 0 Wave days later days later New wave H Injections ny Day of Days Cycle Donor anagement mbryo Collection / xpected esults ynchronization and anagement of ecipients requency Distribution requency of ransferable Beef mbryos % % 0% % 6% % % 0% collections (0 donors) 77 total ova/embryos.9 total ova/embryos per collection. transferable (.9%) embryos per collection Number of embryos poor synchrony tight B N Y O one day N BYO N BYO over several days

4 Proceedings, pplied eproductive trategies in Beef Cattle October -6, 0 taunton, V ynchronization and anagement of ecipients GnH C I D PG check (. d) N Day 0 Day 7 Day 6 GnH PG GnH N C I D hr ynchronization and anagement of ecipients xpected esults ngus arm # ( years) resh embryos rozen embryos Grade ransfers % Pregnant Grade ransfers % Pregnant otal 6.6 otal 9.9 ynchronization and anagement of ecipients xpected esults ynchronization and anagement of ecipients xpected esults rozen embryos Contract ecipient Herds Location otal ransfers Preg rate 66% % 6% 6% 60% % 6% 60% % 60% expect rozen embryos - -60% Grade % Pregnant Best 0-% % lower 0-0% lower What do I tell my clients? verage is OK, but notice the range. resh embryos - expect 7 0% better than frozen embryos orted emen emen orter orted emen educes transferable embryos by ~ 0% Bull dependent Better results in virgins plitting embryos improves output Used with IV

5 Proceedings, pplied eproductive trategies in Beef Cattle October -6, 0 taunton, V IV-In Vitro ertilization Donor s ovaries aspirated transvaginally Oocytes matured and fertilized mbryos transferred IV-In Vitro ertilization dvantages very other week exed semen Use valuable semen on multiple donors Problem donors ble to aspirate pregnant cows IV-In Vitro ertilization Disadvantages Cost Need to transfer fresh Lower conception rates Higher pregnancy wastage Higher incidence of fetal anomalies Logistics In Vitro ystem for mbryo Development DONO spirate oocytes at ur at i o n I V C U L U Day W h at un W h HI P I V L Day 7 CIP egrade & imed ransfer In Vitro ystem for mbryo Development PGNNCY otal hipped otal ecovered otal ransferred 7 Percent ransferred 9% ransfers Grade otal No Pregnant 7 6 % Pregnant 6.7%.%.% 7.% Common easons for Improve genetics vs I Herd expansion arket individual cow families

6 Proceedings, pplied eproductive trategies in Beef Cattle October -6, 0 taunton, V Cost of Production Item Collection ee/donor (drugs, flushing donors, etc) emen ( $0/unit) reezing fee Donor $00.00 $ $0.00 Donors $ $0.00 $00.00 otal $70.00 $0.00 vg. mbryos/collection stimated Cost/ mbryo 7 $0.7 7 ( total) $. a Costs do not include farm labor, overhead or donor maintenance. 6