Name: Xueming Zhao. Professional Title: Professor. Animal embryo biotechnology, mainly including in vitro maturation (IVM), in vitro fertilization

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Name: Xueming Zhao Professional Title: Professor Telephone:86-010-62815892 Fax:86-010-62895971 E-mail: zhaoxueming@caas.cn Website: http://www.iascaas.net.cn/yjspy/dsjj/sssds/dwyzyzypz1/62040.

1 Name: Xueming Zhao Professional Title: Professor Telephone: Fax: Website: Research field Animal embryo biotechnology, mainly including in vitro maturation (IVM), in vitro fertilization (IVF) of mammalian oocytes, vitrification of oocytes and embryos. Work Experience , IAS, CAAS, Professor , IAS, CAAS, Associate Professor , University of Connecticut, Postdoctor , Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Assistant Professor. Educational background China Agricultural University, Animal embryo biotechnology, Ph.D , China Agricultural University, Animal embryo biotechnology, Master , China Agricultural University, Animal Science, Bachelor.

2 Notable honors and service Outstanding young researcher of CAAS. Research description In vitro maturation, fertilization of oocytes and embryo development We successfully establish the high efficiency system of in vitro production of bovine embryos, and the cleavage and blastocyst rates of IVM oocytes reach 85% and 40%.We also investigate the effect of antioxidants on the quality of oocytes and embryos. Notably, we preliminarily discover the mechanism via which melatonin improves the cytoplasmic maturation of bovine oocytes, which may be regulated by melatonin through demethylation approach (2017, Journal of Pineal Research, IF:10.391). Vitrification of mammalian oocytes and embryos We successfully cryopreserve the oocytes and embryos of mouse and bovine by using OPS method and EDFS30 vitrification solution. Mean while, we also focus on the effect of vitrification on the cellular, molecule and epigenetic events of oocytes and embryos. Notably, we find that vitrification leads to the apoptosis of oocytes via mitochondria and melatonin supplementation can inhibit this approach (2016, Journal of Pineal Research, IF:10.391). Funds Until now, I am the PI on nine grants, including two grants of National Natural Science Foundation of China, and I am also sponsored by China Scholarship Council and the outstanding research foundation of CAAS.

3 Reviewers I serve as the peer review of National Natural Science Foundation of China, China Agricultural Science and Technology Award, and five SCI journals, including PLoS One, Reprod.Fertil.Devel., Gene, etc. Publications and patients As the first/corresponding author, I have published 18 SCI papers. The total IF reached 60, and the IF of two papers reached 10. As the first inventor, I have applied for three invention patients. 1. Zhao XM, Wang N, Hao HS, Li CY, Zhao YH, Yan CL, Wang HY, Du WH, Wang D, Liu Y, Pang YW, Zhu HB. Melatonin improves the fertilization capacity and developmental ability of bovine oocytes by regulating cytoplasmic maturation events. J Pineal Res; 2017;e Wang N, Hao HS, Li CY, Zhao YH, Wang HY, Yan CL, Du WH, Wang D, Liu Y, Pang YW, Zhu HB, Zhao XM*. Calcium ion regulation by BAPTA-AM and ruthenium red improved the fertilisation capacityand developmental ability of vitrified bovine oocytes. Sci Rep.2017; 7(1): Wang N, Li CY, Zhu HB, Hao HS, Wang HY, Yan CL, Zhao SJ, Du WH, Wang D, Liu Y, Pang YW, Zhao XM*. Effect of vitrification on the mrna transcriptome of bovine oocytes. Reprod Domest Anim. 2017;52(4): Wei R#, Zhao X#, Hao H, Du W, Zhu H. Embryonic stem-like cells from rabbit blastocysts cultured with melatonin could differentiate into three germ layers in vitro and in vivo. Mol Reprod Dev. 2016;83(11):

4 5.Zhao XM, Cui LS, Hao HS, Wang HY, Zhao SJ, Du WH, Wang D, Liu Y, Zhu HB. Transcriptome analyses of inner cell mass and trophectoderm cells isolated by magnetic- activated cell sorting from bovine blastocysts using single cell RNA-seq. Reprod Domest Anim. 2016;51(5): Zhao XM, Hao HS, Du WH, Zhao SJ, Wang HY, Wang N, Wang D, Liu Y, Qin T, Zhu HB. Melatonin inhibits apoptosis and improves the developmental potential of vitrified bovine oocytes. J Pineal Res 2016;60(2): Zhao XM, Hao HS, Du WH, Zhu HB. Effect of vitrification on the microrna transcriptome in mouse blastocysts. PLoS One. 2015;10:e Zhao XM, Min JT, Du WH, Hao HS, Liu Y, Qin T, Wang D, Zhu HB. Melatonin enhances the in vitro maturation and developmental potential of bovine oocytes denuded of the cumulus oophorus.zygote. 2015;23: Zhao XM, Ren JJ, Zhao SJ, Cui LS, Hao HS, Wang HY, Du WH, Qin T, Liu Y, Wang D, Zhu HB.Apoptosis-Like Events and In Vitro Fertilization Capacity of Sex-Sorted Bovine Sperm. Reprod Domest Anim. 2014;49: Zhao XM, Ren JJ, Du WH, Hao HS, Liu Y, Qin T, Wang D, Zhu HB. Effect of mouse cumulus cells on the in vitro maturation and developmental potential of bovine denuded germinal vesicle oocytes. Zygote. 2014;22: Zhao XM, Ren JJ, Du WH, Hao HS, Wang D, Qin T, Liu Y, Zhu HB. Effect of vitrification on promoter CpG island methylation patterns and expression levels of DNA methyltransferase 1o, histone acetyltransferase 1, and deacetylase 1 in metaphase II mouse oocytes. Fertil Steril. 2013; 100:

5 12. Zhao XM, Ren JJ, Du WH, Hao HS, Wang D, Qin T, Liu Y, Zhu HB. Effect of 5-aza-2 - deoxycytidine on methylation of the putative imprinted control region of H19 during the in vitro development of vitrified bovine two-cell embryos. Fertil Steril.2012;98: Zhao XM, Du WH, Hao HS,Wang D, Liu Y, Qin T, Zhu HB. Effect of vitrification on promoter methylation and the expression of pluripotency and differentiation genes in mouse blastocysts. Mol Reprod Dev. 2012;79: Zhao XM, Du WH, Wang D,Hao HS, Qin T, Liu Y, Zhu HB. Controlled freezing and open-pulled straw (OPS) vitrification of in vitro produced bovine blastocysts following analysis of ATP content and reactive oxygen species (ROS) level. J Integ Agric. 2012;11: Zhao XM, Du WH, Wang D,Hao HS, Liu Y, Qin T, Zhu HB. Effect of cyclosporine pretreatment on mitochondrial function in vitrified bovine mature oocytes. Fertil Steril. 2011; 95: Zhao XM, Du WH, Wang D,Hao HS, Qin T, Liu Y, Zhu HB. Recovery of mitochondrial function and endogenous antioxidant systems in vitrified bovine oocytes during extended in vitro culture. Mol Reprod Dev.2011;78: Zhao XM, Fu XW, Hou YP, Yan CL, Suo L, Wang YP, Zhu HB, Dinnyes A, Zhu SE. Effect of vitrification on mitochondrial distribution and membrane potential in mouse two pronuclear (2-PN) embryos. Mol Reprod Dev. 2009; 76(11): Zhao XM, Quan GB, Zhou GB, Hou YP, Zhu SE. Conventional freezing, straw, and open-pulled straw vitrification of mouse two pronuclear (2-PN) stage embryos. Anim Biotechnol. 2007;18(3):

Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Received 23 January 2013; revised 10 April 2013; accepted 13 April 2013

Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Received 23 January 2013; revised 10 April 2013; accepted 13 April 2013 Iranian Biomedical Journal 17 (3): 123-128 (July 2013) DOI: 10.6091/ibj.1199.2013 The Effect of Vitrification and in vitro Culture on the Adenosine Triphosphate Content and Mitochondrial Distribution of