APCCMS2010 Hong Kong Jan th Applied Biosystems LCMSMS Industry Workshop. Dr Matthew Grigg Asia Pacific Marketing and Support Manager

Transcription

1 APCCMS2010 Hong Kong Jan th Applied Biosystems LCMSMS Industry Workshop Dr Matthew Grigg Asia Pacific Marketing and Support Manager

2 Applied Biosystems LLC and MDS Inc. Joint Owners

3 AB SCIEX Tandem MS Product Portfolio Hybrid Systems 3200 Q TRAP LC/MS/MS System QSTAR ELITE LC/MS/MS System 4000 Q TRAP LC/MS/MS System QTRAP 5500 LC/MS/MS System Food and Beverage Environmental Forensic Clinical Triple Quad Systems Software Solutions API 2000 LC/MS/MS System API 3200 LC/MS/MS System API 4000 LC/MS/MS System Applied Biosystems LLC and MDS Inc. Joint Owners Triple Quad 5500 LC/MS/MS System

4 Use of MS in Clinical Research Applications TDM NBS Biomarker Clin Tox Immunosuppressant Antiretroviral Antipsychotics Anticonvulsive Antiviral Benzodiazepine Diseases Fatty Acid Oxidation Disorder Amino Acid Disorders Organic Acid Steroids T3/T4 Vitamin D Homocysteine Orotic Acid VLCFA General unknown identification Drugs Of Abuse (DOA) Disorders Bile Acid Other Diseases Cancer Etc. API 3200 API 3200 API 3200 to 5000 Q TRAP Applied Biosystems LLC and MDS Inc. Joint Owners

5 Creating Complete Solutions For Routine Analysis Hardware Unique QTrap & TMS Technology Best in Class HPLC Customer Support Installation and training Phone/On-site application support Market Specific Solution Software Cliquid 2.0 Software ChemoView Software imethod Tests Application specific methods MRM Catalogues and Libraries Harnessing the power of Tandem MS: Best-in-class hardware + Easy to use software + Customizable applications solutions Get customers up and running quickly Applied Biosystems LLC and MDS Inc. Joint Owners

6 Cliquid software a new turn key solution Applied Biosystems LLC and MDS Inc. Joint Owners

7 Cliquid Software Goals Goal to provide virtually trainingless solution for LC/MS/MS applications Workflow framework for routine testing Allow users to focus on proper sample prep! Provide expert user with the ability to deploy sophisticated methodologies with little or no training to laboratory technicians Provide complete solutions with pre-configured tests and reports matching regulatory guidelines The Simplest Cliquid 3.0 Workflow Click on Run Samples Choose a Test Build Sample List Name Job and Submit Applied Biosystems LLC and MDS Inc. Joint Owners

8 Cliquid Software and imethod Tests Create custom solutions with easy-to-use Cliquid Software and downloadable pre-configured imethod Tests Food Safety Testing Environmental Monitoring Clinical Research Toxicology Secure and simple four step workflow Pre-configured system suitability tests to ensure optimal systems performance Pre-configured report templates created according to regulatory guidelines Supports English, Japanese and simplified Chinese languages Applied Biosystems LLC and MDS Inc. Joint Owners

9 Increasing Productivity : Increasing Throughput The New Cliquid MPX -2 High Throughput System

10 Cliquid MPX -2 High Throughput System NEW Multiplexing of two HPLCs into a single Mass Spectrometer Applied Biosystems LLC and MDS Inc. Joint Owners

11 Based Upon Customer Input + = More Samples Less Time Savings A new way to increase instrument utilization Applied Biosystems LLC and MDS Inc. Joint Owners

12 Cliquid MPX -2 High Throughput System NEW Key Features: Single point of control software Audit trail / traceability for compliance Intuitive, simple to use method editor Flexible reporting options. Run 1 Real-time instrument status High Pressure HPLC Compatible Run 2 Single vendor solution Simple Cliquid Software workflow Run 3 Supported for use in either Analyst or Cliquid Software Multiplexing of two HPLCs into a single Mass Spectrometer Applied Biosystems LLC and MDS Inc. Joint Owners

13 XIC of +MRM (4 pairs): 210.2/164.2 Da from Sample 9 (STD 5) of Mix_batch_1.wiff (Unknown Ion Source) 8.0e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e Time, min Max. 8.1e4 cps. XIC of +MRM (4 pairs): 210.2/164.2 Da from Sample 9 (STD 5) of Mix_batch_1.wiff (Unknown Ion Source) Max. 8.1e4 cps e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e Time, min XIC of +MRM (4 pairs): 210.2/164.2 Da from Sample 9 (STD 5) of Mix_batch_1.wiff (Unknown Ion Source) 8.0e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e Time, min Max. 8.1e4 cps. XIC of +MRM (4 pairs): 210.2/164.2 Da from Sample 9 (STD 5) of Mix_batch_1.wiff (Unknown Ion Source) 8.0e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e Time, min Max. 8.1e4 cps. XIC of +MRM (4 pairs): 210.2/164.2 Da from Sample 9 (STD 5) of Mix_batch_1.wiff (Unknown Ion Source) 8.0e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e Time, min Max. 8.1e4 cps. XIC of +MRM (4 pairs): 210.2/164.2 Da from Sample 9 (STD 5) of Mix_batch_1.wiff (Unknown Ion Source) 8.0e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e Time, min Max. 8.1e4 cps. XIC of +MRM (4 pairs): 210.2/164.2 Da from Sample 9 (STD 5) of Mix_batch_1.wiff (Unknown Ion Source) 8.0e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e Time, min Max. 8.1e4 cps. XIC of +MRM (4 pairs): 210.2/164.2 Da from Sample 9 (STD 5) of Mix_batch_1.wiff (Unknown Ion Source) 8.0e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e Time, min Max. 8.1e4 cps. How Multiplex LC Works Designed to Support Two Parallel LC Streams into a Single MS idle acquire from stream 1 acquire from stream 2 wait for sync signal Example of staggered injections from two HPLC systems running the same method 1 inject to stream N send sync signal Applied Biosystems LLC and MDS Inc. Joint Owners

14 Method Editor View A single screen within Analyst Software to input all HPLC parameters Applied Biosystems LLC and MDS Inc. Joint Owners

15 Instrument Status View A second screen to monitor the real time instrument status Applied Biosystems LLC and MDS Inc. Joint Owners

16 Example Analysis

17 Example: Vitamin D Analysis XIC of +MRM (5 pairs): 383.4/229.2 Da ID: Vit-D3-2 from Sample 4 (Vitamin D Recipe QC2) of 2009_03_12_173017_12Mar09_VitD_ e4 8.0e4 Max. 4.5e4 cps. 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 OH-Vitamin-D 3 4 th Injection 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 OH-Vitamin-D 2 2.0e4 1.5e4 1.0e XIC of +MRM (5 pairs): 383.4/229.2 Da ID: Vit-D3-2 from Sample 50 (Vitamin D Recipe QC2) of Time, 2009_03_12_173017_12Mar09_VitD_00... min Max. 5.5e4 cps. 8.3e4 8.0e4 7.5e4 7.0e4 6.5e4 6.0e4 5.5e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 2.5e4 2.0e4 1.5e4 1.0e th Injection Time, min Two fold increase in throughput: 100 injections, Singleplex Run Time:>6 hr 30 min 100 injections, Multiplex Run Time: 3 hr 30 min Time saved: 3 hr Applied Biosystems LLC and MDS Inc. Joint Owners

18 Example: Amino Acid Analysis XIC of +MRM (72 pairs): 330.1/115.1 amu Expected RT: 2.2 ID: PSer_IS from Sample 3 (Sample003) of Mutiple batches aa45.wiff (Turb... Max. 6.0e4 cps. 5.2e5 5.0e5 4.8e5 4.6e5 4.4e5 4.2e5 4.0e5 3.8e5 3.6e5 3.4e5 3.2e5 3.0e5 2.8e5 2.6e5 2.4e5 2.2e5 2.0e5 1.8e5 1.6e5 1.4e5 1.2e5 1.0e5 8.0e e4 4.0e4 2.0e Time, min Two fold increase in throughput: 10 AA45 sample injections Singleplex Run Time : >200 min Multiplex Run Time: 89 min Time saved: 111 min Applied Biosystems LLC and MDS Inc. Joint Owners

19 Increasing Productivity : Increasing Throughput Online Sample Prep -

20 Conditioning Equilibration Sample Application Washes Evaporation Reconstitution N 2 OFF-LINE ON-LINE Analytical Column Analytical Column Injection On-line vs Off-line SPE Drying Analyte elution Switch valve Applied Biosystems LLC and MDS Inc. Joint Owners

21 Symbiosis PICO System The Pico system is integrated with the full line of ABI MS systems Applied Biosystems LLC and MDS Inc. Joint Owners

22 Symbiosis On-line Technique How It Operates Applied Biosystems LLC and MDS Inc. Joint Owners

23 Software Integration Optimal Workflow Main features Analyst 1.5 Security & audit trailing (21 CFR Part 11 compliant) Seamless integration in to Analyst 1.5 Method editor Batch editor Applied Biosystems LLC and MDS Inc. Joint Owners

24 imethod TM Test for Analysis of Four Immunosuppressants XICof +MRM(6pairs): /425.4DaID: CyclosporineAfromSample 33 (Cal 6) of 2008_12_12_182119_Immunosuppressant.wiff (Turbo... Max. 2.3e5 cps. Method Info: API 3200 LC/MS/MS System Spark PICO system with Mistral column oven AB POROS R1 sorbent Phenomenex Luna 5 u Phenyl-Hexyl 50 x 2.1 mm HPLC Analyte LLOQ ng/ml Cyclosporine 47.1 Tacrolimus 2.3 Sirolimus 2.4 Everolimus Applied Biosystems LLC and MDS Inc. Joint Owners 2.3e5 2.2e5 2.1e5 2.0e5 1.9e5 1.8e5 1.7e5 1.6e5 1.5e5 1.4e5 1.3e5 1.2e5 1.1e5 1.0e5 9.0e4 8.0e4 7.0e4 6.0e4 5.0e4 4.0e4 3.0e4 2.0e4 1.0e4 0.0 Tacrolimus (44.0 ng/ml) Everolimus (44.1 ng/ml) Ascomycin (IS) Sirolimus (48.2 ng/ml) 1.35 Cyclosporine A (1743 ng/ml) Cyclosporine D (IS) Time, min Chromatogram of Chromsystems Whole Blood Calibrator Level 6

25 imethod TM Test for 25-Mono-Hydroxy-Vitamin D2 and 25-Mono-Hydroxy- Vitamin D3 in Human Plasma XIC of +MRM (3 pairs): 383.1/211.1 Da ID: MHVD 3 from Sample 5 (100 ng/ml) of cal curve.wiff (Heated Nebulizer) Max. 4.3e4 cps. 4.2e Method Info: API 4000 LC/MS/MS System In te n s ity, c p s 4.0e4 3.8e4 3.6e4 3.4e4 3.2e4 3.0e4 2.8e4 2.6e4 2.4e4 2.2e4 2.0e4 1.8e4 1.6e4 1.4e4 1.2e4 1.0e Time, min Chromatogram of a 100 ng/ml MHVD3 in plasma Spark PICO system with Mistral column oven Spark HySphere C8EC SE sorbent SPE cartridge Merck Chromolith SpeedROD RP-18e 50x4.6 mm HPLC column LLOQ: 5 ng/ml Applied Biosystems LLC and MDS Inc. Joint Owners MHVD3 Cal Curve, ng/ml

26 orclinicalresearch/index.htm Applied Biosystems LLC and MDS Inc. Joint Owners

27 Analysis by LC-MS/MS Development of an imethod TM Test

28 How imethod Test was Developed Literature search on most recent publication and reference levels to set goals Sample volume; run time; sample preparation; detection limits; curve and QC ranges Method development: established detail requirements for method validation and sample analysis selectivity, accuracy, precision, recovery, reproducible and matrix effects Three runs to establish method documents Intra-day and inter-day; over 100 samples for each batch Three verification runs from different labs and scientists Feed back and modify method and documents Final imethod test documentation Chemical and reagent supply; sample preparation procedure; HPLC conditions; MS/MS conditions; example chromatogram; tricks and tips Applied Biosystems LLC and MDS Inc. Joint Owners

29 Trace Level Estrogen Analysis Applied Biosystems LLC and MDS Inc. Joint Owners

30 The Importance of Estrogens Estrogen is an entire class of related female sex hormones: estrone (E1) estradiol (E2), and estriol (E3) Estrone (E1) is widespread throughout the body. It is the only one of estrogens that s present in any amount in women after menopause. Estradiol (E2) is the primary sex hormone of childbearing women. It is formed from developing ovarian follicles. Estradiol is responsible for female characteristics and sexual functioning. Also, estradiol is important to women s bone health. Estradiol contributes to most gynecologic problems and even female cancers. Estriol (E3) is made from the placenta. It s important only during pregnancy Applied Biosystems LLC and MDS Inc. Joint Owners

31 Summary of Estrogens Reference Levels Population Estrone (E1) Estradiol (E2) Estriol(E3) (Pregnancy) Adult Male pg/ml pg/ml < 2ng/mL Premenopausal Female pg/ml pg/ml Postmenopausal Female pg/ml <10 pg/ml (Pregnancy) ng/ml (non-pregnancy) <2 ng/ml Target LOQ 5 pg/ml 5 pg/ml < 10 pg/ml ULQ pg/ml pg/ml 2000 pg/ml TIETZ Textbook of Clinical Chemistry and Molecular Diagnostics; 4 th Edition Estradiol: LC-MSMS test: 300K- 500K samples in US for one year Reference: Steroids 73 (2008) ; Steroids 73 (2008) Applied Biosystems LLC and MDS Inc. Joint Owners

32 The Fragmentation of Estradiol on APCI LC-MSMS Estradiol H2O Ring open fragments -H2O Applied Biosystems LLC and MDS Inc. Joint Owners

33 The Fragmentation of Estradiol on ESI Neg LC-MSMS (Less interferences from other steroids in neg mode) Estradiol _Neg No ring open fragments Applied Biosystems LLC and MDS Inc. Joint Owners

34 Factors Were Considered and Evaluated Different organic solvent combination for liquid-liquid extraction Derivatization with dansyl has also been evaluated High background and interference IS impurity interference and stability IS is not very stable at low concentration Interferences from other endogenous level steroids No major interference from other steroids to E1 and E2 in neg ionization mode Run time: 6min Column wash with high organic is important for the roughness of assay Different phase columns have been tested C18, C8, Bi-phenyl, hydro-rp Gemini ESI or APCI APCI pos is less sensitive than ESI neg with mobile phase modifier Non-specific binding High non-specific binding for E1 and E2 Glass labware and injection vial are recommended Applied Biosystems LLC and MDS Inc. Joint Owners

35 Conditions: Verified Instrumentation Estrone and Estradiol: API 5000 and AB SCIEX Triple Quad 5500 Mass Spectrometers Shimadzu Prominence 20A and Agilent 1200 integrated systems Method Information 500 µl sample volume required Liquid-liquid extraction without derivatization Clinically relevant dynamic range of pg/ml 6 minute total run time Applied Biosystems LLC and MDS Inc. Joint Owners

36 Estrone and Estradiol: Lower Limit of Quantitation Estrone 5 pg/ml LLOQ, S/N=22 Estradiol 5 pg/ml LLOQ, S/N= Applied Biosystems LLC and MDS Inc. Joint Owners

37 Sensitivity: LQC in Human Serum E3 at LQC 30 pg/ml S/N= Applied Biosystems LLC and MDS Inc. Joint Owners

38 Estrone and Estradiol: Precision and Accuracy Estrone (E1) Sample (pg/ml) %CV % Accuracy Estradiol (E2) Sample (pg/ml) %CV % Accuracy Day 1-3 Day 1-3 LLOQ (5) Low QC (15) High QC (100) ULOQ (500) Day 1-3 Day 1-3 LLOQ (5) Low QC (15) 4 96 High QC (100) ULOQ (500) Estrone (inter-day) LLOQ CV s 9%,QC s CV s 5% Accuracy deviation 4% Estradiol (inter-day) LLOQ CV s 10%,QC s CV s 5% Accuracy deviation 4% Applied Biosystems LLC and MDS Inc. Joint Owners

39 Estrone and Estradiol: Linearity Estrone Estradiol Clinically relevant dynamic range pg/ml, R Applied Biosystems LLC and MDS Inc. Joint Owners

40 Roughness of Assay: Estrone Low QC Analyte to IS ratio Analyte peak area Retention Time Applied Biosystems LLC and MDS Inc. Joint Owners

41 Estrone and Estradiol: Summary The method was verified internally across laboratories for analytical analysis LLOQ with suitable signal to noise (20) Inter-day Precision and Accuracy LLOQ CVs 10%,QCs CV s 5% Accuracy Deviation ±4% Control samples at clinically relevant low and high concentrations (5-500 pg/ml) with linear response r Two steps liquid liquid extraction without derivatization 6 minutes run time Applied Biosystems LLC and MDS Inc. Joint Owners

42 Summary AB SCIEX is extending its leadership in the mass spectrometry market to new markets and applications Simplifying mass spectrometry for applied markets and clinical research Will continue on complete workflow solutions Systems Software Methods and Kits Training Support Applied Biosystems LLC and MDS Inc. Joint Owners

43 Acknowledgements Beth Frenandez Tania Sasaki Elliott Jones Lisa Sapp David Lavorato Adam Latawiec; Theresa Lee; Peter Lodenquai; Susan Leonard Applied Biosystems LLC and MDS Inc. Joint Owners

44 Legal Acknowledgments For Research Use Only. Not for use in diagnostic procedures The trademarks mentioned herein are the property of either Life Technologies Corporation, Applied Biosystems/MDS Analytical Technologies, or otherwise, their respective owners 2009 Applied Biosystems LLC and MDS Inc. Joint Owners. All Rights Reserved Applied Biosystems LLC and MDS Inc. Joint Owners