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6 Huntingdon Life Sciences Page 6. Final Report TABLE OF CONTENTS Page 1136 COVER PAGE...1 GLP STATEMENT...2 SIGNATURE PAGE...3 QUALITY ASSURANCE STATEMENT...4 SUMMARY...5 TABLE OF CONTENTS INTRODUCTION MATERIALS AND METHODS 2.1. Study Management Study Dates Experimental Outline Justifications Test Substance Test Animals Animal Assignment Animal Identification Veterinary Care Husbandry During Non-Exposure Periods Husbandry During Exposure Periods Test Substance Preparation Test Substance Administration and Chamber Operation Exposure Chamber Sampling Experimental Evaluations Postmortem Evaluation of Dams and Pups Statistical Analysis Data Storage Regulatory References Protocol Deviations RESULTS AND DISCUSSION 3.1. Chamber Monitoring Mortality and Pregnancy Clinical Observations Body Weights...31

7 Huntingdon Life Sciences Page 7. Final Report TABLE OF CONTENTS Page Feed Consumption Organ Weights Macroscopic Pathology Cesarean Section Data Fetal External Examination Data CONCLUSION...32 REFERENCES...33 FIGURES 1. Diagram of 1000-Liter Whole-Body Exposure Chamber and Generation System - Group Diagram of 1000-Liter Whole-Body Exposure Chamber and Generation System - Groups 2, 3 & Mean Body Weights - Males Mean Body Weights - Females Mean Feed Consumption - Males Mean Feed Consumption - Females...39 TABLES 1. Summary of Survival and Pregnancy Summary of In-Chamber Observations Summary of Clinical Observations...42 Summary of Clinical Observations (Males)...43 Summary of Clinical Observations During Gestation (Females) Mean Body Weights (Males)...45 Summary of Gestation Body Weights (Females) Mean Body Weight Gain (Males)...47 Summary of Gestation Body Weight Gain (Females) Mean Feed Consumption (Males)...49 Summary of Gestation Feed Consumption (Females) Mean Organ Weight Values Summary of Necropsy Observations...63 Summary of Necropsy Observations (Males)...64 Summary of Maternal Necropsy Observations (Females) Summary of Cesarean Section Data Summary of Gravid Uterine Weight and Net Body Weight Change...69

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10 Page 1140 Huntingdon Life Sciences Page 10 Final Report DATE OF ANIMAL RECEIPT 7 October 2004 (Males)* - Experimental Start Date 15 & 18 October 2004 (Females) EXPOSURE INITIATION 20 October 2004** (Experimental Start Date), 21 and 22 October EXPOSURE TERMINATION 2, 3 and 4 November TERMINAL SACRIFICES 3,4 and 5 November EXPERIMENTAL TERMINATION 5 November 2004 (Date of Last Data Collection) STUDY COMPLETION 19 February 2010 (Date Final Report is signed by the Study Director) *as per OECD GLPs; ** as per EPA GLPs

11 Page 1141 Huntingdon Life Sciences Page 11 Final Report 2.3. EXPERIMENTAL OUTLINE Number of Animals Test Exposure Group Substance Level" Initial Necropsy ppm M F M F 1 Air Control Liquified Petroleum Gas Liquified Petroleum Gas Liquified Petroleum Gas "Exposures were 6 hours per day for 7 consecutive days per week for 2 weeks for a total of exactly 14 exposures. Exposure levels are expressed as ppm of test substance. The exposures were conducted via whole-body exposure. M = male; F = female (these animals were timed-mated and were exposed from gestation days 6 to 19). The first day of exposures was defined as day 0 of the study (males). The first day of gestation was defined as day 0 of the study (females) JUSTIFICATIONS ROUTE, DURATION AND FREQUENCY OF ADMINISTRATION The inhalation route is one of the potential routes of human exposure to this test substance. The duration of the study and frequency of the exposures were considered to be the minimum necessary for determining exposure levels for a subsequent 13 week inhalation study and a developmental inhalation study EXPOSURE LEVEL SELECTION The exposure levels were selected based on establishing (for safety reasons) the high exposure level as no more than 50% of the lower explosion limit (LEL = 2.1 % = ppm) for the test substance.

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13 Huntingdon Life Sciences Page 13 Final Report EXPIRATION DATE 31 December ANALYSIS Documentation of the identity, strength, purity, composition, storage stability, and method of synthesis, fabrication, and/or derivation of the test substance and the maintenance of these records were the responsibility of the Sponsor STORAGE Ambient conditions ARCHIVAL SAMPLE The sponsor arranged for storage of an archival sample at a contract archival facility (EPL Archives, Inc., Terminal Drive, Sterling, VA 20166). Since multiple studies ( & ) were conducted with the same test substance, a common archival sample was taken and appropriately labeled. The testing facility arranged for safe transportation on 15 September 2005 of the archival sample to the archival facility, in compliance with all applicable regulations DISPOSITION 2.6. TEST ANIMALS Following completion of the final study with this test substance, the testing facility informed the sponsor of the volume of remaining test substance as well as the number of test substance containers. The sponsor then instructed the testing facility to ship the test substance and containers to Chevron and this was done on 11 January No test substance or container (even empty ones) will be disposed of until the issuance of the final report SPECIES Albino Rats (Outbred) VAF/Plus CD (Sprague-Dawley derived) [Crl: CD BR] Crl: CD (SD) IGS BR Page 1143

14 Huntingdon Life Sciences Page 1144 Page 14 Final Report SUPPLIER Charles River Laboratories Kingston, N ew York NUMBER OF ANIMALS Received: 88 total (44 males, 44 females) Placed on test: 80 total (40 males, 40 females) AGE AT RECEIPT Approximately 6 weeks (males) Approximately 75 to 85 days in age (females) AGE AT INITIATION OF DOSING Approximately 8 weeks (males) Approximately 78 to 90 days in age (females) WEIGHT AT INITIATION OF DOSING (GRAMS) Male: Female: Mean Range 234 to to 282 Individual weights of animals placed on test were within ±20% of the mean weight for each sex ACCLIMATION PERIOD Animals were acclimated for approximately 2 weeks for male animals. Female animals were received on days 1, 2 or 3 of gestation and were quarantined until start of exposures on gestation day 6. All animals were checked for viability twice daily. Prior to assignment to study, all animals were examined to ascertain suitability for study.

15 Huntingdon Life Sciences Page 1145 Page 15 Final Report 2.7. ANIMAL ASSIGNMENT More animals than required for the study were purchased and acclimated. Male animals considered suitable for study on the basis of pretest physical examinations, body weight data and any other pretest evaluations, were distributed into 4 groups of 10 males by a computerized random sort program so that body weights means for each group were comparable. Female animals considered suitable for study on the basis of pretest physical examinations were randomized into groups on gestation day 4 using a method that ranked the gestation day 4 body weights and randomly assigned each female animal within the block into groups. Disposition of all animals not utilized in the study is maintained in the study file ANIMAL IDENTIFICATION Each animal was assigned a temporary identification number upon receipt. Each rat was identified with a metal ear tag bearing its assigned animal number. The assigned animal number plus the study number comprised the unique animal number for each animal. In addition, each cage was provided with a cage card that was color-coded for dose level identification and contained study number and animal number information VETERINARY CARE Animals were monitored by the technical staff for any conditions requiring possible veterinary care and treated as necessary ANIMAL HUSBANDRY DURING NON-EXPOSURE PERIODS FACILITIES MANAGEMENT/ANIMAL HUSBANDRY Currently acceptable practices of good animal husbandry were followed e.g., Guide for the Care and Use of Laboratory Animals; National Academy Press, Huntingdon Life Sciences, East Millstone, New Jersey is fully accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC) HOUSING Animals were individually housed in suspended, stainless steel wire mesh cages during the study.

16 Huntingdon Life Sciences Page Page 16 Final Report FEED Certified Rodent Diet, No (Meal) (PMI Nutrition International, st. Louis, Missouri) was available without restriction 7 days a week except during exposures. Fresh feed was presented weekly FEED ANALYSIS Analysis of each feed lot used during this study was performed by the manufacturer. Results are maintained on file by the Testing Facility. There were no known contaminants in the feed which were expected to interfere with the results of this study WATER Water (Elizabethtown Water Company, Westfield, New Jersey) was available without restriction via an automated watering system WATER ANALYSIS Water analyses are conducted by Elizabethtown Water Company, Westfield, New Jersey (Raritan-Millstone Plant) to ensure that water meets standards specified under the EPA Federal Safe Drinking Water Act Regulations (40 CFR Part 141). In addition, water samples are collected biannually from representative rooms in the Testing Facility; chemical and microbiological water analyses are conducted on these samples by a subcontract laboratory. Results of all water analyses are maintained on file at the Testing Facility. There were no known contaminants in the water which were expected to interfere with the results of this study ENVIRONMENTAL CONDITIONS LightlDark Cycle A twelve hour light/dark cycle controlled via an automatic timer was provided.

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