Optimization of saccharification conditions of prebiotic extracted jackfruit seeds
|
|
- Annis Fields
- 5 years ago
- Views:
Transcription
1 Paper Code: fb005 TIChE International Conference 0 November 0, 0 at Hatyai, Songkhla THAILAND Optimization of saccharification conditions of prebiotic extracted jackfruit seeds Sininart Chongkhong *, Bancha Lolharat, Abdullateef Doramae, Pakamas Chatpattananondh Department of Chemical Engineering, Faculty of Engineering, Prince of Songkla University, Songkhla, 90, Thailand * sininart.c@psu.ac.th Abstract In this work, response surface methodology (RSM) was used to determine the optimum saccharification condition in the hydrolysis steps for the ethanol production from prebiotic extracted jackfruit seeds. Three parameters, which were investigated, were gluco-amylase amount ( %w) temperatures (50 70 C) and time ( min). To apply the quadratic model, the two-level-three-factor array ( ) experiment was explained by using a central composite design (CCD). The determination coefficient, R value, for reducing sugar content in the hydrolyzed product was greater than For this statistical analysis, it showed that the factors effected on the reducing sugar content significantly. Base on response surface, the optimum condition for saccharification process was 0. %w gluco-amylase amount at the 50 C incubation temperature and 60 min. It could provide 5. g/l of the highest amount reducing sugar content in the hydrolyzed product. Keyword: Jackfruit seeds, Glucoamylase, Enzymatic Hydrolysis, Response surface methodology
2 TIChE International Conference 0 November 0, 0 at Hatyai, Songkhla THAILAND. Introduction The commercial utilization of amylase enzyme is the hydrolysis of a starchy biomass as raw material for many industries. On a dry basis, agricultural products that compose mainly of starch, hydrolyzed to glucose, offer a good resource for fermentation processes []. The enzymatic hydrolysis of starchy materials can be used in the liquefaction and saccharification processes conventionally. The components of the prebiotic extracted jackfruit seeds are 5.0% starch, 4.99% crude proteins, 0.5% fat, 0.75% ash and 58.8% moisture []. Ethanol production from the prebiotic extracted jackfruit seeds, a starchy material, can be carried out by using enzymes in pretreatment and hydrolysis processes. The starch can be transformed into fermentable sugar by the liquefaction using alpha-amylase enzyme, and then by the saccharification using gluco-amylase enzyme in the hydrolysis process. After that the sugar is converted into ethanol using yeast in the fermentation process. Likewise, the non-reducing sugars at the tail end of the amylase and amylopectin molecules can be hydrolyzed into the reducing sugars by using the gluco-amylase in the saccharification [ 4]. The several starchy materials were investigated by using the gluco-amylase for the hydrolysis step [5]. The optimum operating condition of enzymatic hydrolysis of cassava waste using the glucoamylase was at ph 4.5, 60 C for 4 hrs [6]. The response surface methodology (RSM) has been increasingly used to optimize the ethanol production process [, 6-7]. RSM has been widely used to evaluate and understand the interactions between different physiological and nutritional parameters. This research is intended to determine the optimum conditions (incubation time, temperature and enzyme concentration) for saccharification process of prebiotic extracted jackfruit seeds by using RSM.. Materials and Methods.. Materials and Microorganisms Prebiotic extracted jackfruit seeds are obtained from SME-OTOP research group, Prince of Songkla University, Thailand. Alpha-amylase (Aspergillus cryzae) and Gluco-amylase (Aspergillus niger) were bought from the Sigma-Aldrich company... Pretreatment The crushed seeds, which were about mm particle size, were boiled at 85 C for 5 min. Then they were cooled down until it reached a room temperature.... Liquefaction process The 5 g of the seeds mixed with 5 ml water were poured in 50 ml screw-capped bottles with 0.7 %w Alpha-amylase. The liquefaction process was carried out at an initial ph 6.0, 80 C for 40 min in the oil both with shaking rate of 00 rpm.... Saccharification process The temperature of the liquefied product was lowered to 60 C. The saccharification process was investigated at C for min with %w glucoamylase by using RSM. The reactions were kept at ph 4.5 with the shaking rate of 00 rpm. The hydrolysates (saccharificated products) were separated by centrifuge at 5,000 rpm for 5 min to obtain the clear liquid product before reducing sugar content analysis... Analytical methods The reducing sugars were assayed in terms of glucose by DNS method using a double beam UV-Vis spectrophotometer (model HP 845) with UV-Visible Chem-Station software []..4. Experimental design and optimization Central composite design (CCD) was used to find the optimum reducing sugar concentrations in the hydrolyzed products. Time (, min), temperature (, C), and gluco-amylase amount (, %w) were chosen as the independent variables (Tables and ). The variables were coded according to Eq. (): i xi, xi i =,,,, k () x j where x i are the dimensionless coded level of variables, i are the actual value, x i are the average of the high and low level values and x are the high value minus the low value of that the variables. The significance of each variable influencing hydrolysis process was determined by Student s t test with 95% confidence levels. Table.. Variables in the experimental design. Variables Coded levels Time (min) Temperature ( C) Gluco-amylase (%w) j fb005-
3 Predicted Values (g/l) TIChE International Conference 0 November 0, 0 at Hatyai, Songkhla THAILAND Table.. The central composite design matrix employed for three independent variables (Actual values are given in Table ). Run no. x x x Reducing sugar concentration (Y, g/l) in the hydrolyzed product was a desirable output. To obtain optimum conditions, 7 experiments in Table were studied. A mathematic model for predicting the output yields by ANOVA, Eq. (), was created from the roles of parameters, their interactions and statistical analysis. Y 0 () Where Y is the predicted response;,, are the independent variables; b 0 is the offset term; b, b, b are the linear effects; b, b, b are the square effects; and b, b, b are the cross effects of the interaction terms. A correction of the polynomial model Eq. () can be verified with the determination coefficient (R ) and the F-test for the statistical significance.. Results and Discussion.. Response surface analysis for optimization of three factors The trials and results for central composite design are shown in Table. The response surface and contour curves were obtained by using Eq. (): Y () Table.. Experimental and predicted yields for reducing sugar Run no. Experimental Reducing sugar (g/l) Predictive Fig. shows a satisfactory correlation between experimental values and predictive values. As can be seen from the figure, the predicted data of the response from the empirical model agreed well with the observed ones in the range of the operating variables R = Experimental Values (g/l) Fig.. Parity plot for the distribution of experimental vs. predicted values of reducing sugar. The results from using Eq. () showed a high determination coefficient, R = 0.96, which implied that the model was a good fit with the experiments. It gave 96.% predicting efficiency. The adjusted R value corrects the R value for the sample size and for the number of terms. A high value of adjusted determination coefficient (adjusted R = 0.9) advocates for a high significance of the model. fb005-
4 TIChE International Conference 0 November 0, 0 at Hatyai, Songkhla THAILAND As illustrated in Tables 4 and 5, a P value of 0.05 for any factor in analysis of variance (ANOVA) indicates a significant effect of the corresponding factors on the response. The model F value of 0.50 and values of probability > F indicated that the model terms are significant Table.4. Coefficients, t-statistics and significance probability of the model. Term Coefficient Value Standard error t-value P-value Constant b Time b Temperature b Gluco-amylase b Time x time b Temperature x temperature b Gluco-amylase x gluco-amylase b Time x temperature b Temperature x gluco-amylase b Time x gluco-amylase b Table.5. ANOVA for the full quadratic model. Source of variation Sum of squares (SS) Degrees of freedom (DF) Mean squares (MS) F-value Probe > F Regression Residual LOF Error Pure Error Interactions among the factors Fig.. Response surface of temperature vs. time (the enzymatic hydrolysis of prebiotic extracted jackfruit seeds with 0.% glucoamylase). Effects of temperatures and time on the reducing sugar amount (hydrolysis yield) are shown in Fig.. The reducing sugar content was decreased with temperature ranging from 50 to 70 C as the content was increased with the increasing in time from 40 to 480 min. Fig.. Response surface of temperature vs. glucoamylase amount (the enzymatic hydrolysis for 60 min) The amount of gluco-amylase use in the process was varied from 0.05 to 0. %w for 40 to 480 min. It found that the highest reducing sugar content could be obtained at the centre point of this factors (0. %w gluco-amylase and 60 min). fb005-4
5 TIChE International Conference 0 November 0, 0 at Hatyai, Songkhla THAILAND Fig. 4. Response surface of temperatures vs. gluco-amylase amount (the enzymatic hydrolysis at 60 C) Fig. 4 shows the effects of temperatures and amylase amount on reducing sugar content at 60 C. The formation of sugar content was reduced when temperature was raised while the sugar content was increased with amylase amount from 0.05 to 0. %w. Further increase of amylase amount did not significantly increase the amount of reducing sugar. 4. Conclusions suitability for the hydrolysis of wheat starch. Process Biochem. 9 (00) [] G.L. Miller, Use of dinitrosalicyclic acid reagent for determination of reducing sugar, Anal. Chem. (959) [] B. Lolharat, S. Chongkhong, P. Chetpattananondh, Optimization of saccharification condition for the ethanol production from prebiotic extracted jackfruit seed. The 5 th PSU-UNS International Conference on Engineering and Technology (ICET), Prince of Songkla University, Faculty of Engineering, Phuket, May - (0). [4] H. Anto, U.B. Trivedi and K.C. Patel, Glucoamylase production by solid-state fermentation using rice flake manufacturing waste products as substrate, Bioresource Technol. 97 (0) (006) [5] Q. Wang,. Wang,. Wang, H. Ma, Glucoamylase production from food waste by Aspergillus niger under submerged fermentation. Process Biochem. 4 (008) [6] S. O'Brien, Y.-J. Wang, Susceptibility of annealed starches to hydrolysis by [alpha]-amylase and glucoamylase. Carbohyd. Polym. 7 (008) [7] S. Teerapatr, K. Lerdluk and S. La-aied, Approach of Cassava Waste Pretreatments for Fuel Ethanol Production in Thailand, Chulalongkorn J. Sci. Res. (006). [8] I.-L. Shih, C.-Y. Kuo, F.-C. Hsieh, S.-S. Kao, C. Hsieh, Use of surface response methodology to optimize culture conditions for iturin A production by Bacillus subtilis in solid-state fermentation, Journal of the Chinese Institute of Chemical Engineers 9 (008) [9] G.L. Miller, Use of dinitrosalicyclic acid reagent for determination of reducing sugar, Anal. Chem. (959) The response surface methodology (RSM) was successfully used to determine the optimum conditions for the hydrolysis of the prebiotic extracted jackfruit seeds. The saccharification process could increase the significantly reducing sugar concentration before operating in the next fermentation step for ethanol production. A quadratic model, established in terms of hydrolysis: temperature, time and gluco-amylase amount, was proved usefully to predict the yield of saccharification process. The recommended for hydrolysis condition was 0.% enzyme concentration at 50 C for 60 min. It provided 5. g/l reducing sugar content. 5. Acknowledgements The authors gratefully acknowledge the financial support from the Graduate School of Prince of Songkla University, DoE (Discipline of Excellent chemical engineering) and NRCT 0. References [] S.K. Soni, A. Kaur, J.K. Gupta, A solid state fermentation based bacterial [alpha]-amylase and fungal glucoamylase system and its fb005-5
Optimizing conditions for enzymatic clarification of prebiotic extracted jackfruit seeds using response surface methodology
The 5 th PSU-UNS International Conference on Engineering and Technology (ICET-0), Phuket, May -, 0 Prince of Songkla University, Faculty of Engineering Hat Yai, Songkhla, Thailand 90 Optimizing conditions
More informationOPTIMIZATION OF RICE BRAN HYDROLYSIS AND KINETIC MODELLING OF XANTHAN GUM PRODUCTION USING AN ISOLATED STRAIN
International Journal of Science, Environment and Technology, Vol. 4, No 2, 2015, 285 292 ISSN 2278-3687 (O) 2277-663X (P) OPTIMIZATION OF RICE BRAN HYDROLYSIS AND KINETIC MODELLING OF XANTHAN GUM PRODUCTION
More informationEnzymatic Bioconversion and Fermentation of Corn Stover at High-solids Content for Efficient Ethanol Production
National Technical University of Athens School of Chemical Engineering Biotechnology Laboratory Industrial Waste & Wastewater Treatment & Valorization Enzymatic Bioconversion and Fermentation of Corn Stover
More informationENZYMATIC HYDROLYSIS OPTIMIZATION OF SWEET POTATO (Ipomoea batatas) PEEL USING A STATISTICAL APPROACH
Brazilian Journal of Chemical Engineering ISSN 0104-66 Printed in Brazil www.abeq.org.br/bjche Vol. 0, No. 0, pp. 467-476, July - September, 01 ENZYMATIC HYDROLYSIS OPTIMIZATION OF SWEET POTATO (Ipomoea
More informationOPTIMUM HYDROLYSIS CONDITIONS OF CASSAVA STARCH FOR GLUCOSE PRODUCTION
OPTIMUM HYDROLYSIS CONDITIONS OF CASSAVA STARCH FOR GLUCOSE A. Ayodeji Ayoola* A. Opeyemi Adeeyo** C. Vincent Efeovbokhan*** D. Adeola Olasimbo**** PRODUCTION Abstract: Acid and enzymatic hydrolysis of
More informationIn this study, effect of different high-boiling-organic solvent (ethanolamine, diethylene glycol and
ISESCO JOURNAL of Science and Technology Vol. 12 No 21 High Boiling Solvent Pre-treatment of Hazelnut Shells for Enzymatic Hydrolysis Emir Zafer Hoşgün, Berrin Bozan Anadolu University, Engineering Faculty,
More informationIJREAT International Journal of Research in Engineering & Advanced Technology, Volume 1, Issue 2, April-May, 2013 ISSN:
A Comparative Overview of Ethanol Production from Cereal Grains and Potato by Enzymatic Treatment Soumitra Banerjee 1, Debalina Kundu 2 Dept of Food Technology, Techno India Saltlake, Kolkata 700091 Abstract
More informationTakahiro Noda National Agricultural Research Center for Hokkaido Region (NARCH), JAPAN Workshop Japan-New Zealand (JST), 11 October 2010, Tokyo.
National Agriculture and Food Research Organization The enzymatic digestibility and phosphate content in potato starches Takahiro Noda National Agricultural Research Center for Hokkaido Region (NARCH),
More informationImprovement of enzymatic hydrolysis of a marine macro-alga by dilute acid hydrolysis pretreatment
Improvement of enzymatic hydrolysis of a marine macro-alga by dilute acid hydrolysis pretreatment Parviz Yazdani 1*, Keikhosro Karimi 1,2, Mohammad J. Taherzadeh 2 1 Department of Chemical Engineering,
More informationEnzyme use for corn fuel ethanol production. Luis Alessandro Volpato Mereles
Enzyme use for corn fuel ethanol production Luis Alessandro Volpato Mereles July 12 th, 2007 Agenda Global Biofuel Outlook Novozymes at a glance What are enzymes Using Enzymes to produce Fuel Ethanol from
More informationScreening of Rice Straw Degrading Microorganisms and Their Cellulase Activities
Research 83 KKU Sci. J.37 (Supplement) 83-88 (2009) Screening of Rice Straw Degrading Microorganisms and Their Cellulase Activities Abstract Atcha Boonmee 1,2* Rice straw is one of the most abundant agricultural
More informationEXTRACTION OF THERMO-STABLE ALPHA AMYLASE FROM FERMENTED WHEAT BRAN
BIOLOGIA 2001, 47 (1&2), PP 47 52 ISSN 0006 3096 EXTRACTION OF THERMO-STABLE ALPHA AMYLASE FROM FERMENTED WHEAT BRAN *HAMAD ASHRAF, IKRAM UL HAQ, AND JAVED IQBAL Biotechnology Research Laboratory, Department
More informationOptimization of Operating Variables in the Fermentation of Citric acid using Response Surface Methodology
2011 2nd International Conference on Chemical Engineering and Applications IPCBEE vol. 23 (2011) (2011) IACSIT Press, Singapore Optimization of Operating Variables in the Fermentation of Citric acid using
More informationProduction of Thermostable and Ca +2 Independent α-amylases from Halphilic Bacteria
International Journal of Biotechnology and Biochemistry ISSN 0973-2691 Volume 12, Number 2 (2016) pp. 153-159 Research India Publications http://www.ripublication.com Production of Thermostable and Ca
More informationHydrothermal pretreatment of biomass for ethanol fermentation
Hydrothermal pretreatment of biomass for ethanol fermentation Yukihiko Matsumura Hiroshima University 1 Dec. 10-12, 2012 JAPANESE-DANISH JOINT WORKSHOP Future Green Technology Hakata, Japan 緒言 First and
More informationResearch & Reviews in. Optimization of glucose syrup production process using sabrang potato starch (Coleus tuberosus Benth) by hydrolysis enzimatic
ISSN : 0974-7532 Research & Reviews in Volume 9 Issue 2 BioSciences RRBS, 9(2), 2014 [49-55] Optimization of glucose syrup production process using sabrang potato starch (Coleus tuberosus Benth) by hydrolysis
More informationAPPLICATION OF FTIR-ATR SPECTROSCOPY FOR DETERMINATION OF GLUCOSE IN HYDROLYSATES OF SELECTED STARCHES
RESEARCH PAPERS FACULTY OF MATERIALS SCIENCE AND TECHNOLOGY IN TRNAVA SLOVAK UNIVERSITY OF TECHNOLOGY IN BRATISLAVA 10.2478/rput-2013-0019 2013, Volume 21, Special Number APPLICATION OF FTIR-ATR SPECTROSCOPY
More informationUSING CENTRAL COMPOSITE DESIGNS - RESPONSE SURFACE METHODOLOGY TO OPTIMIZE INVERATSE ACTIVITY CONDITIONS FOR FRUCTOSE PRODUCTION
Conference proceedings of Biotechnology for Green Solutions and Sustainable Environment: 8-87, 010 USING CENTRAL COMPOSITE DESIGNS - RESPONSE SURFACE METHODOLOGY TO OPTIMIZE INVERATSE ACTIVITY CONDITIONS
More informationChemical and Microbial Hydrolysis of Sweet Sorghum Bagasse for Ethanol Production
Chemical and Microbial Hydrolysis of Sweet Sorghum Bagasse for Ethanol Production Anusith Thanapimmetha 1,2, Korsuk Vuttibunchon 1, Maythee Saisriyoot 1,2, Penjit Srinophakun 1,2 * 1 Bioprocess Laboratory,
More informationOPTIMIZATION OF ENZYMATIC HYDROLYSIS OF RAMIE DECORTICATION WASTE-BASED CELLULOSE USING RESPONSE SURFACE METHODOLOGY
OPTIMIZATION OF ENZYMATIC HYDROLYSIS OF RAMIE DECORTICATION WASTE-BASED CELLULOSE USING RESPONSE SURFACE METHODOLOGY Laeli Kurniasari 1*, Suwardiyono 1, Renan Subantoro 2, Indah Hartati 1 1 Chemical Engineering,
More informationMolecular Structure and Function Polysaccharides as Energy Storage. Biochemistry
1 1.Objectives Dr. Vijaya Khader Dr. MC Varadaraj To understand how polysaccharides act as energy source To understand the structure and energy generation process from glycogen To understand the structure
More information5 Optimisation of Process Parameters of L- asparaginase production by isolate SI091
Optimisation of Process Parameters of L-asparaginase production by isolate SI91 69 5 Optimisation of Process Parameters of L- asparaginase production by isolate SI91 5.1 Introduction Success of bioprocess
More informationOPTIMISATION OF XYLOSE PRODUCTION USING XYLANASE
Int. J. Chem. Sci.: 8(2), 2010, 909-913 OPTIMISATION OF XYLOSE PRODUCTION USING XYLANASE T. SATHISH a and N. Y. S. MURTHY * Department of Biotechnology, Malla Reddy Engineering College, HYDERABAD (A.P.)
More informationOptimizing the Conversion of Pretreated Sila Sorghum Stalks to Simple Sugars Using Immobilized Enzymes
Optimizing the Conversion of Pretreated Sila Sorghum Stalks to Simple Sugars Using Immobilized Enzymes Wiseman Tumbo Ngigi Department of Chemical & Process Engineering, Moi University, P.O. Box 39-31,
More informationKinetics analysis of β-fructofuranosidase enzyme. 1-Effect of Time Incubation On The Rate Of An Enzymatic Reaction
Kinetics analysis of β-fructofuranosidase enzyme 1-Effect of Time Incubation On The Rate Of An Enzymatic Reaction Enzyme kinetics It is the study of the chemical reactions that are catalyzed by enzymes.
More informationHEMICELLULASE from ASPERGILLUS NIGER, var.
HEMICELLULASE from ASPERGILLUS NIGER, var. Prepared at the 55th JECFA (2000) and published in FNP 52 Add 8 (2000), superseding tentative specifications prepared at the 31st JECFA (1987) and published in
More informationThe Application of Détente Instantanée Contrôlée (DIC) Technology to Minimize the Degradation Rate of Glucose
International Proceedings of Chemical, Biological and Environmental Engineering, Vol. 88 (2015) DOI: 10.7763/IPCBEE. 2015. V88. 6 The Application of Détente Instantanée Contrôlée (DIC) Technology to Minimize
More informationContinuous Starch Liquefaction Through In-line Static Mixer Reactor
The 3 rd Conference of Starch Technology Continuous Starch Liquefaction Through In-line Static Mixer Reactor S. Chamsart 1, 2, A. Sriprasit 1, S. Thungkal 1, 3 and Y. Waiprib 1, 4 1BBERG Bangsaen Biochemical
More informationAMYLOGLUCOSIDASE from ASPERGILLUS NIGER, var.
AMYLOGLUCOSIDASE from ASPERGILLUS NIGER, var. SYNONYMS INS No. 1100 Prepared at the 59 th JECFA (2002) and published in FNP 52 Add 10 (2002), superseding tentative specifications prepared at the 55 th
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 211, 1 (3):124-129 ISSN: 2248 9215 Production of Alkaline Protease by Bacillus subtilis (MTCC7312) using Submerged
More informationΒ-FRUCTOFURANOSIDASE ENZYME
KINETICS ANALYSIS OF Β-FRUCTOFURANOSIDASE ENZYME 2-The effects of enzyme concentration on the rate of an enzyme catalyzed reaction. Systematic names and numbers β-fructofuranosidase (EC 3.2.1.26) Reactions
More informationProduction of 5-Aminolevulinic Acid from Monosodium Glutamate Effluent by Halotolerant Photosynthetic Bacterium (Rhodobacter capsulatus SS3)
Production of -Aminolevulinic Acid from Monosodium Glutamate Effluent by Halotolerant Photosynthetic Bacterium (Rhodobacter capsulatus SS) Amornrat Chaikritsadakarn,*, Poonsuk Prasertsan, and Piyarat Boonsawang
More informationInternational Journal on Future Revolution in Computer Science & Communication Engineering ISSN: Volume: 4 Issue:
Application of the Variance Function of the Difference Between two estimated responses in regulating Blood Sugar Level in a Diabetic patient using Herbal Formula Karanjah Anthony N. School of Science Maasai
More informationCHAPTER NO. TITLE PAGES
BRIEF CONTENTS CHAPTER NO. TITLE PAGES PREFACE 1 1 INTRODUCTION 3 2 REVIEW OF LITERATURE 6 3 MATERIALS & METHODS 70 4 OBSERVATIONS & RESULTS 110 5 DISCUSSION 222 6 SUMMARY & CONCLUSIONS 243 BIBLIOGRAPHY
More informationInternational Conference on Material Science and Application (ICMSA 2015)
International Conference on Material Science and Application (ICMSA 215) Antioxidant Synergistic Effect and Formulation Optimization of Several Common Natural Pigments Yi-Hua JIANG 1, a, *, Xin-Long JIANG
More informationCELLULASE from PENICILLIUM FUNICULOSUM
CELLULASE from PENICILLIUM FUNICULOSUM Prepared at the 55th JECFA (2000) and published in FNP 52 Add 8 (2000), superseding tentative specifications prepared at the 31st JECFA (1987) and published in FNP
More informationLevan production by Bacillus licheniformis NS032 using sugar beet molasses Gojgic-Cvijovic Gojgic Cvijovic Gordana1*, Jakovljevic Dragica1, Kekez Bran
Levan production by Bacillus licheniformis NS032 using sugar beet molasses Gojgic-Cvijovic Gojgic Cvijovic Gordana1*, Jakovljevic Dragica1, Kekez Branka2, Beskoski Vladimir2, Vrvic M. Miroslav2 1Institute
More informationEffect of Different Combinations of Soybean and Wheat Bran on Enzyme Production from Aspergillus oryzae S.
Available online at www.sciencedirect.com APCBEE Procedia 00 (2012) 000 000 Conference title Effect of Different Combinations of Soybean and Wheat Bran on Enzyme Production from Aspergillus oryzae S. Chuenjit
More informationResponse surface methodology for the optimization of kojic acid production by Aspergillus flavus using Palmyra sap as a carbon source
014; (5): 5-57 ISSN: 31-91 www.biosciencejournals.com EJBB 014; (5): 5-57 Received: 13-11-014 Accepted: 9-11-014 Kayitha Bala Durga Devi Payala Vijayalakshmi Bapatla Veerendra Kumar Correspondence: Kayitha
More informationEffect of process conditions on high solid enzymatic hydrolysis of pre-treated pine
Effect of process conditions on high solid enzymatic hydrolysis of pre-treated pine Abstract Anders Josefsson Department of Chemical Engineering, Lund University, Sweden 213-6-12 In this study a relatively
More information) and time (x 3. ), temperature (x 2
26 ASIAN JOURNAL OF DAIRY & FOOD RESEARCH MATERIALS AND METHODS Banana fruits: Bananas (Musa sp.) of variety Robusta were procured at the maturity stage 1(all green) from selected banana orchards near
More informationEFFECT OF HEMICELLULOSE LIQUID PHASE ON THE ENZYMATIC HYDROLYSIS OF AUTOHYDROLYZED EUCALYPTUS GLOBULUS WOOD
S05-036 EFFECT OF HEMICELLULOSE LIQUID PHASE ON THE ENZYMATIC HYDROLYSIS OF AUTOHYDROLYZED EUCALYPTUS GLOBULUS WOOD Romaní, Aloia; Ruiz, Héctor A. *; Pereira, Francisco B; Domingues, Lucília; Teixeira,
More informationExperiment 1. Isolation of Glycogen from rat Liver
Experiment 1 Isolation of Glycogen from rat Liver Figure 35: FIG-2, Liver, PAS, 100x. Note the presence of a few scattered glycogen granules (GG). Objective To illustrate the method for isolating glycogen.
More informationOptimization of processing parameters for extraction of total, insoluble and soluble dietary fibers of defatted rice bran
doi:10.9755/ejfa.v5i8.14513 FOOD SCIENCE AND NUTRITION Optimization of processing parameters for extraction of total, insoluble and soluble dietary fibers of defatted rice bran Cheickna Daou 1,,3* and
More informationSupplementary information to Municipal solid waste as carbon and energy source for Escherichia coli
Supplementary information to Municipal solid waste as carbon and energy source for Escherichia coli Erica Rosander, Maria Svedendahl Humble and Andres Veide KTH Royal Institute of Technology, School of
More informationIsolation and Screening of Amylase Producing Fungi
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 4 (2017) pp. 783-788 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.604.098
More informationInfluence of extrusion-saccharification parameters to filtration of syrup made of enzymatic extruded degermed corn
IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) e-issn: 9-8, p-issn: 9-7. Volume, Issue Ver. I (November 7), PP 6-66 www.iosrjournals.org Influence of extrusion-saccharification parameters
More informationOPTIMIZATION OF PROTEASE PRODUCTION FROM HUSK OF VIGNA MUNGO BY BACILLUS SUBTILIS NCIM 2724 USING STATISTICAL EXPERIMENTAL DESIGN
http://www.rasayanjournal.com Vol.4, No.1 (2011), 159-164 ISSN: 0974-1496 CODEN: RJCABP OPTIMIZATION OF PROTEASE PRODUCTION FROM HUSK OF VIGNA MUNGO BY BACILLUS SUBTILIS NCIM 2724 USING STATISTICAL EXPERIMENTAL
More informationOptimization of A Strong Wheat Flour Quality With A Low Alpha-Amylase Content by Using Response Surface Methodology
Optimization of A Strong Wheat Flour Quality With A Low Alpha-Amylase Content by Using Response Surface Methodology Silvia Mironeasa Faculty of Food Engineering, Stefan cel Mare University of Suceava,
More informationEffect of ph on the production of protease by Fusarium oxysporum using agroindustrial waste
Biotechnological Communication Biosci. Biotech. Res. Comm. 8(1): 78-83 (2015) Effect of ph on the production of protease by Fusarium oxysporum using agroindustrial waste Rupali R. Deshmukh and N. N. Vidhale*
More informationMass flow every 24h BCRL SSCF. Enzymes Biomass, Water EH. Liquid Ferm. broth
Fig. S1 BCRL SHF Mass flow every 24h Enzymes Biomass, Water EH hydrolysate Ferm. Ferm. broth Enzymes Biomass, Water EH Whole hydrolysate SSCF Ferm. broth Solids buildup Solids buildup BCRL SHF EH (ml)
More informationFunctional Properties of Foods. Database and Model Prediction
Functional Properties of Foods. Database and Model Prediction Nikolaos A. Oikonomou a, Magda Krokida b a Department of Chemical Engineering, National Technical University of Athens, Athens, Greece (nikosoik@central.ntua.gr)
More informationExperiment 9. NATURE OF α-amylase ACTIVITY ON STARCH
Experiment 9 NATURE OF α-amylase ACTIVITY ON STARC In Experiment 1 we described the action of α-amylase on starch as that of catalyzing the hydrolysis of α-1,4-glucosidic bonds at random in the interior
More informationMost of the ethanol that is used as a biofuel in this country is produced from corn.
Chem 251 Ethanol from Corn Most of the ethanol that is used as a biofuel in this country is produced from corn. In this experiment you will make ethanol from frozen corn kernels using a process similar
More informationTHE RELATIONSHIP BETWEEN TWO METHODS FOR EVALUATING FIVE-CARBON SUGARS IN EUCALYPTUS EXTRACTION LIQUOR
THE RELATIONSHIP BETWEEN TWO METHODS FOR EVALUATING FIVE-CARBON SUGARS IN EUCALYPTUS EXTRACTION LIQUOR Congcong Chi, a,b* Zeng Zhang, a Weiwei Ge, a and Hasan Jameel b Alkaline pre-extraction and hydrothermal
More informationMIXED XYLANASE, β-glucanase ENZYME PREPARATION, produced by a strain of HUMICOLA INSOLENS
MIXED XYLANASE, β-glucanase ENZYME PREPARATION, produced by a strain of HUMICOLA INSOLENS New specifications prepared at the 61st JECFA (2003) and published in FNP 52 Add 11 (2003). An ADI not specified
More informationHYDROLYSIS OF GINGER (ZINGIBER OFFICINALE ROSCOE) STARCH: SEARCH FOR PROCESS CONDITIONS USING THE SURFACE RESPONSE METHODOLOGY
HYDROLYSIS OF GINGER (ZINGIBER OFFICINALE ROSCOE) STARCH: SEARCH FOR PROCESS CONDITIONS USING THE SURFACE RESPONSE METHODOLOGY Silvânia R. M. Moreschi 1, Ademir J. Petenate 2, M. Angela A. Meireles 1,
More informationThis chapter deals with the evaluation of alpha amylase inhibitory
This chapter deals with the evaluation of alpha amylase inhibitory activity of different extracts isolated from leaves of Aloe vera L. and leaves of Azadiracta indica A Juss. collected from Bharatpur and
More informationWhat is it? Ear of Teosinite
What is it? Ear of Teosinite The amazing corn kernel just became more amazing! Introducing Enogen Corn Corn has Come a Long Way Over the Course of the Last 7000 Years That Little Kernel is full of little
More informationIMPROVEMENT OF WHEAT FLOUR DOUGH RHEOLOGY BY ΑLPHA -AMYLASE AND PROTEASE COMBINATION
Journal homepage: www.fia.usv.ro/fiajournal Journal of Faculty of Food Engineering, Ştefan cel Mare University of Suceava, Romania Volume XIII, Issue 4-2014, pag. 309-316 IMPROVEMENT OF WHEAT FLOUR DOUGH
More informationSIMULATION AND CONTROL OF ENZYMATIC HYDROLYSIS REACTOR OF SOLUBLE POTATO STARCH BY ASPERGILLUS NIGER GLUCOAMYLASE FOR FERMENTATION PROCESS FEED
SIMULATION AND CONTROL OF ENZYMATIC HYDROLYSIS REACTOR OF SOLUBLE POTATO STARCH BY ASPERGILLUS NIGER GLUCOAMYLASE FOR FERMENTATION PROCESS FEED R. R. FONSECA, T. S. S. DANTAS and F. V. DA SILVA State Universityof
More informationProduction of Reducing Sugars from Hydrolysis of Napier Grass by Acid or Alkali
Doi: 10.12982/cmujns.2017.0003 CMU J. Nat. Sci. (2017) Vol. 16(1) 31 Production of Reducing Sugars from Hydrolysis of Napier Grass by Acid or Alkali Duangkanok Tanangteerapong*, Thanawat Tunjaroensin,
More informationCollege of Food Science and Engineering, Beijing University of Agriculture, Beijing, , China
2018 International Conference on Modeling, Simulation and Optimization (MSO 2018) ISBN: 978-1-60595-542-1 Optimization of the Ultrasound-assisted Extraction of Saponins from Germinated Quinoa and the Evaluation
More informationHeterotrophic Growth of Chlorella sp. KKU-S2 for Lipid Production using Molasses as a Carbon Substrate
2011 International Conference on Food Engineering and Biotechnology IPCBEE vol.9 (2011) (2011)IACSIT Press, Singapoore Heterotrophic Growth of Chlorella sp. KKU-S2 for Lipid Production using Molasses as
More informationEfficient hydrolysis of raw starch and ethanol fermentation: a novel raw starch digesting glucoamylase from Penicillium oxalicum
DOI 10.1186/s13068-016-0636-5 Biotechnology for Biofuels RESEARCH Open Access Efficient hydrolysis of raw starch and ethanol fermentation: a novel raw starch digesting glucoamylase from Penicillium oxalicum
More informationRecycling of yeast cells for simultaneous saccharification and fermentation of liquefied starch of rice flour
Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 2012, 2 (1):127-134 ISSN: 2248 9215 CODEN (USA): EJEBAU Recycling of yeast cells for simultaneous saccharification
More informationInternational Journal of Modern Chemistry, 2012, 1(1): 1-8 International Journal of Modern Chemistry
International Journal of Modern Chemistry, 2012, 1(1): 1-8 International Journal of Modern Chemistry Journal homepage: www.modernscientificpress.com/journals/ IJMChem.aspx ISSN: 2165-0128 Florida, USA
More informationMathematical Modeling for the Prediction of Liquid Glucose and Xylose Produced From Cassava Peel
American Journal of Engineering Research (AJER) e-issn: 232-847 p-issn : 232-936 Volume-6, Issue-5, pp-274-28 www.ajer.org Research Paper Open Access Mathematical Modeling for the Prediction of Liquid
More informationEFFECT OF CARBON SOURCES ON FORMATION OF a-amylase AND GLUCOAMYLASE BY
J. Gen. App!. Microbiol,, 21, 51-59 (1975) EFFECT OF CARBON SOURCES ON FORMATION OF a-amylase AND GLUCOAMYLASE BY CLOSTRIDIUM ACETOBUTYLICUM BURT ENSLEY, JOHN J. McHUGH, AND LARRY L. BARTON Department
More informationMinimizing Wash Water Usage After Acid Hydrolysis Pretreatment of Biomass
University of Arkansas, Fayetteville ScholarWorks@UARK Biological and Agricultural Engineering Undergraduate Honors Theses Biological and Agricultural Engineering 5-2013 Minimizing Wash Water Usage After
More informationHealth and Medical Education, Tehran, Iran 3 Administration for Supervision and Evaluation of Food and Beverages, Food and Drug Organization,
ESTIMATING AND MODELING EFFECTS OF CITRIC ACID, ASCORBIC ACID AND SODIUM CHLORIDE ON GLUTEN EXTRACTION FROM WHEAT FLOUR USING RESPONSE SURFACE METHODOLOGY (RSM) Lahordi N. 1, *Eskandari S.,3, Karimian
More informationAvailable online at ScienceDirect. Procedia Environmental Sciences 31 (2016 )
Available online at www.sciencedirect.com ScienceDirect Procedia Environmental Sciences 31 (2016 ) 5 534 The Tenth International Conference on Waste Management and Technology (ICWMT) The content variation
More informationColour of starch-iodine complex as index of retrogradability of starch pastes
Vol. 8(5), pp. 89-93 May, 2014 DI:10.5897/AJPAC2014.0571 Article Number: 802AA7844885 ISSN 1996-0840 Copyright 2014 Author(s) retain the copyright of this article http://www.academicjournals.org/ajpac
More informationMark J. Anderson, Patrick J. Whitcomb Stat-Ease, Inc., Minneapolis, MN USA
Journal of Statistical Science and Application (014) 85-9 D DAV I D PUBLISHING Practical Aspects for Designing Statistically Optimal Experiments Mark J. Anderson, Patrick J. Whitcomb Stat-Ease, Inc., Minneapolis,
More informationAstaxanthin Production by Phaffia rhodozyma Fermentation of. Cassava Residues Substrate
Astaxanthin Production by Phaffia rhodozyma Fermentation of Cassava Residues Substrate Jinsong Yang 1a, Haisheng Tan 1b, Rui Yang 1c, Xiaohuan Sun 1d,Hairui Zhai 1e, Kaimian Li f * Hainan University,Haikou,
More informationJournal of Chemical and Pharmaceutical Research, 2014, 6(3): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 0, (3):37-391 Research Article ISSN : 0975-734 CODEN(USA) : JCPRC5 Effects of twin-screw extrusion on soluble dietary fibre
More informationAlpha-amylase Adsorption on Raw Starch and Its Relation to Raw Starch Digestion* By R.M. SANDSTEDT and SeinoSuke UEDA**
Journal of the Japanese Society of Starch Science, Vol. 17, No. 1 (1969) 215 Alpha-amylase Adsorption on Raw Starch and Its Relation to Raw Starch Digestion* By R.M. SANDSTEDT and SeinoSuke UEDA** Department
More informationProduction and stabilization of amylases from Aspergillus niger
Mycosphere Production and stabilization of amylases from Aspergillus niger Monga M, Goyal M *, Kalra KL and Soni G Department of Biochemistry and Department of Microbiology, Punjab Agricultural University,
More informationINFLUENCE of PARTICLE SIZE on CITRIC ACID PRODUCTION by Aspergillus niger USING RICE CHAFF and
INFLUENCE of PARTICLE SIZE on CITRIC ACID PRODUCTION by Aspergillus niger USING RICE CHAFF and SESAMUM OIL CAKE as SUBSTRATES S M Gopinath, Department of Biotechnology, Acharya Institute of Technology,
More informationBIO- DEGRADABLE COMPOSITE MADE FROM STARCH AND COCONUT FIBER : MECHANICAL STRENGTH AND BIODEGRATION CHRACTERSTICS
BIO- DEGRADABLE COMPOSITE MADE FROM STARCH AND COCONUT FIBER : MECHANICAL STRENGTH AND BIODEGRATION CHRACTERSTICS 55 Rahul Sen*, N.C.Upadhayay**, Upender Pandel*** *Research Scholar **Associate Professor
More informationRenewable Carbon-Feedstock to Industrial Chemicals: Producing Renewable Materials from Granular Starch
S E 1 E 2 E 3 E 4 E n Continuous Biocatalytic Systems P Renewable Carbon-Feedstock to Industrial Chemicals: Producing Renewable Materials from Granular Starch Manoj Kumar, PhD Sr. Scientist, Research &
More informationOptimization of Enzymatic Hydrolysis of Manihot esculenta Root Starch by α-amylase and Glucoamylase Using Response Surface Methodology
Optimization of Enzymatic Hydrolysis of Manihot esculenta Root Starch by α-amylase and Glucoamylase Using Response Surface Methodology F. A. Aderibigbe 1*, A. L. Adejumo 2, R. U. Owolabi 3 and A.N. Anozie
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com Der Chemica Sinica, 2013, 4(1):67-74 ISSN: 0976-8505 CODEN (USA) CSHIA5 Analysis of Abakaliki Rice Husks N. B. Ekwe Chemical Engineering Department, University
More informationUSE OF ENZYMES IN HYDROLYSIS OF MAIZE STALKS. Ivo Valchev, Sanchi Nenkova, Petya Tsekova, and Veska Lasheva
USE OF ENZYMES IN HYDROLYSIS OF MAIZE STALKS Ivo Valchev, Sanchi Nenkova, Petya Tsekova, and Veska Lasheva Lignocellulosic biomass is the most abundant organic raw material in the world. Cellulose and
More informationResearch on Extraction Process of Gallic Acid from Penthorum chinense Pursh by Aqueous Ethanol
Green and Sustainable Chemistry, 2015, 5, 63-69 Published Online May 2015 in SciRes. http://www.scirp.org/journal/gsc http://dx.doi.org/10.4236/gsc.2015.52009 Research on Extraction Process of Gallic Acid
More informationChina. Keywords: Synergistic Effects, Antioxidant, Ginkgo Leaves Flavonoid, Loquat Leaves Flavonoid, Response Surface Method(RSM).
2016 International Conference on Energy Development and Environmental Protection (EDEP 2016) ISBN: 978-1-60595-360-1 Antioxidant Synergistic Effect and Formulation Optimization of Ginkgo Leaves Flavonoid
More informationPreparation of Glucose Syrup from Waxy Corn by Enzymatic Process
Dagon University Research Journal 2011, Vol. 3 Preparation of Glucose Syrup from Waxy Corn by Enzymatic Process Thin Thin Naing * Abstract was prepared from waxy corn. Enzymatic hydrolysis of prepared
More informationWorld Journal of Pharmaceutical and Life Sciences WJPLS
wjpls, 2018, Vol. 4, Issue 1, 80-84 Research Article ISSN 2454-2229 Rajarajan et al. WJPLS www.wjpls.org SJIF Impact Factor: 4.223 PRODUCTION AND CHARACTERIZATION OF AMYLASE FROM ASPERGILLUS NIGER Dr.
More informationAmylase Production from Potato and Banana Peel Waste
Int.J.Curr.Microbiol.App.Sci (213) 2(11): 41-414 ISSN: 2319-776 Volume 2 Number 11 (213) pp. 41-414 http://www.ijcmas.com Original Research Article Amylase Production from Potato and Banana Peel Waste
More informationA Model for the Starch Enzymatic Hydrolysis based on the Boltzmann Equation
A Model for the Starch Enzymatic Hydrolysis based on the Boltzmann Equation A.PATSIOURA, E.REPPAS, G.MANIATIS and V.GEKAS Department of Environmental Engineering, Technical University of Crete, Polytechnioupolis,
More informationProduction and Optimization of Protease from Aspergillus niger and Bacillus subtilis using Response Surface Methodology
IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) ISSN: 2455-264X, Volume 2, Issue 7 (Nov. Dec. 2016), PP 01-07 Production and Optimization of Protease from Aspergillus niger and Bacillus subtilis
More informationRESPONSE SURFACE MODELING AND OPTIMIZATION TO ELUCIDATE THE DIFFERENTIAL EFFECTS OF DEMOGRAPHIC CHARACTERISTICS ON HIV PREVALENCE IN SOUTH AFRICA
RESPONSE SURFACE MODELING AND OPTIMIZATION TO ELUCIDATE THE DIFFERENTIAL EFFECTS OF DEMOGRAPHIC CHARACTERISTICS ON HIV PREVALENCE IN SOUTH AFRICA W. Sibanda 1* and P. Pretorius 2 1 DST/NWU Pre-clinical
More informationSaccharification of corncob using cellulolytic bacteria - Titi Candra Sunarti et al.
Saccharification of corncob using cellulolytic bacteria - Titi Candra Sunarti et al. Figure 2. (a) (b) (c) (d) Microscopic structures of (a) corncob, (b) delignified corncob, (c) cellulose fraction, (d)
More informationStudy on moisture content of substrate (Sesamum oil cake and Rice chaff) on the yield of Citric acid
Int.J.Curr.Microbiol.App.Sci (14) 3(5): 138-144 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-776 Volume 3 Number 5 (14) pp. 138-144 http://www.ijcmas.com Original Research
More informationThe Use of Novel Enzyme Accelerant Technology in Reducing Costs and Increasing Yields in Ethanol Production.
The Use of Novel Enzyme Accelerant Technology in Reducing Costs and Increasing Yields in Ethanol Production. Ken Matthews. Business Development Manager Eka Chemicals Inc. 1 Pulp, Paper and More. The Use
More informationWe are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists. International authors and editors
We are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists 3,500 108,000 1.7 M Open access books available International authors and editors Downloads Our
More informationDirect Fermentation of Potato Starch to Ethanol by Cocultures of Aspergillus niger and Saccharomyces cerevisiaet
APPLID AND NVIRONMNTAL MICROBIOLOGY, Nov. 1986, p. 155-159 99-224/86/11155-5$2./ Copyright C 1986, American Society for Microbiology Vol. 52, No. 5 Direct Fermentation of Potato Starch to thanol by Cocultures
More informationBioconversion of solid food wastes to ethanol
Analyst, March 1998, Vol. 123 (497 502) 497 Bioconversion of solid food wastes to ethanol Jothi V. Kumar *a, Abolghasem Shahbazi b and Rose Mathew a a Department of Chemistry, North Carolina A&T State
More informationOptimization of Acidic Hydrolysis of Sweet Potato Peels To Produce Fermentable Sugar
IOSR Journal of Engineering (IOSRJEN) ISSN (e): 2250-3021, ISSN (p): 2278-8719 Vol. 08, Issue 6 (June. 2018), V (I) PP 20-26 www.iosrjen.org Optimization of Acidic Hydrolysis of Sweet Potato Peels To Produce
More information