Supporting Information. Mutational analysis of a phenazine biosynthetic gene cluster in
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1 Supporting Information for Mutational analysis of a phenazine biosynthetic gene cluster in Streptomyces anulatus 9663 Orwah Saleh 1, Katrin Flinspach 1, Lucia Westrich 1, Andreas Kulik 2, Bertolt Gust 1, Hans-Peter Fiedler 2 and Lutz Heide *1 Address: 1 Pharmaceutical Institute, University of Tübingen, Auf der Morgenstelle 8, Tübingen, Germany and 2 Institute of Microbiology and Infection Medicine, University of Tübingen, Auf der Morgenstelle 28, Tübingen, Germany Lutz Heide - heide@uni-tuebingen.de * Corresponding author Fax: Analytical data, complete list of genes in cosmid ppzos04, and PCR primers S1
2 Figure S1: Production of endophenazine A and endophenazine E by S. coelicolor M512(ppzOS04) after cultivation for 3, 5 and 7 days in 24 deep-well plates. S2
3 Figure S2: 1 H- 1 H COSY ( ), HSQC ( ) and HMBC ( ) correlations from 2D NMR experiments for endophenazine E. S3
4 Figure S3: Reduction of ubiquinone to ubiquinol, catalyzed by cytochrome d ubiquinol oxidase, and reduction of phenazine-1-carboxylic acid to dihydro-phenazine-1-carboxylic acid, possibly catalyzed by PpzZ1/PpzZ2 of the endophenazine biosynthetic gene cluster of Streptomyces anulatus S4
5 Figure S4: Extracted ion chromatograms for the mass of endophenazine B ([M + H] + = 323) for the heterologous host S. coelicolor M512 with the cosmid ppzos04 carrying the intact phenazine biosynthetic gene cluster from S. anulatus and the heterologous host with the constructs ppzos10-11 and ppzos S5
6 Table S1: Genes identified on cosmid ppzos04 from S. anulatus Gene aa Proposed function Orthologue identified by BLAST Identity/ Acc. Number search Similarity % orf1 333 serine protease putative serine protease, Streptomyces 93/98 ZP_ roseosporus NRRL orf2 342 aspartate-semialdehyde ASD2, Streptomyces griseus subsp. 97/99 YP_ dehydrogenase griseus NBRC orf3 115 putative transcriptional modulator Streptomyces roseosporus NRRL 71/87 ZP_ orf4 870 aminopeptidase N Streptomyces griseus subsp. griseus 98/99 YP_ NBRC ppzp 299 prenyltransferase EpzP, Streptomyces cinnamonensis 57/71 ADQ43372 mk 345 mevalonate kinase MK, Streptomyces cinnamonensis 76/87 ADQ43373 mdpd 351 diphosphomevalonate decarboxylase Streptomyces sp. CL190 77/84 BAB07791 pmk 371 phosphomevalonate kinase Streptomyces sp. CL190 72/80 BAB07792 ippi 363 isopentenyl-diphosphate deltaisomerase hmgr hydroxy-3-methylglutaryl coenzyme A reductase hmgs hydroxy-3-methylglutaryl CoA synthase Streptomyces sp. CL190 79/88 Q9KWG2 HMGR, Streptomyces cinnamonensis 89/94 ADQ43377 LPMA, Streptomyces sp. SN-1061M 85/92 ADC96649 ppzt 327 putative acetoacetyl-coa synthase Streptomyces sp. KO /87 BAD86806 ppzu 221 flavodoxin flavoprotein WrbA, Streptomyces violaceusniger Tü /79 YP_ ppzv 206 putative TetR-family regulator EpzV, Streptomyces cinnamonensis 64/76 ADQ43382 ppzm 340 N-methyltransferase EpzM, Streptomyces cinnamonensis 77/86 ADQ43384 ppza 168 phenazine biosynthesis EpzA2, Streptomyces cinnamonensis 83/88 ADQ43385 ppzg 233 FMN-dependent oxidase EphzG, Streptomyces cinnamonensis 68/77 CAL34111 ppzf 279 trans 2,3-dihydro 3- hydroxyanthranilate isomerase ppze amino-2-desoxy-isochorismate synthase ppzd 207 2,3-dihydro-3-hydroxy-anthranilate (DHHA) synthase ppzc deoxy-D-arabino-heptulosonic acid 7-phosphate synthase EpzF, Streptomyces cinnamonensis 87/94 ADQ43387 EphzE, Streptomyces cinnamonensis 82/87 CAL34110 EphzD, Streptomyces cinnamonensis 85/92 CAL34109 EphzC, Streptomyces cinnamonensis 82/89 CAL34108 ppzb 162 phenazine biosynthesis EphzB, Streptomyces cinnamonensis 83/88 CAL34107 ppzk 419 FAD-dependent oxidoreductase FAD-dependent pyridine nucleotidedisulfide oxidoreductase ppzl 107 ferredoxin ferredoxin, Rhodopseudomonas palustris HaA2 ppzy 290 transcriptional regulator transcriptional regulator, Streptomyces lividans TK24 55/66 YP_ /68 YP_ /74 ZP_ S6
7 ppzz1 430 cytochrome d ubiquinol oxidase, subunit I cytochrome d ubiquinol oxidase, subunit I, Stackebrandtia nassauensis DSM ppzz2 344 cytochrome d ubiquinol oxidase, cytochrome d ubiquinol oxidase, subunit subunit II II, Stackebrandtia nassauensis DSM ppzr1 563 ABC transporter cysteine ABC transporter permease, Thermobispora bispora DSM ppzr2 585 ABC transporter cysteine ABC transporter permease, Streptosporangium roseum DSM orf allantoicase putative allantoicase, Streptomyces griseus subsp. griseus NBRC /66 YP_ /60 YP_ /68 YP_ /65 YP_ /99 YP_ S7
8 Table S2: Sequences of the primers used to construct the cosmids ppzos10, ppzos11 and ppzos Cosmid Primer Sequence ppzos10 18A9_L_for 5 -GCC AAG CAG GGC AAG CCC GCC CAC GAG TTC TCG GTG AAG TCT AGA ATT CCG GGG ATC CGT CGA CC-3 ppzos11 ppzos21 ppzos22 ppzos23 ppzos24 ppzos25 ppzos26 ppzos27 ppzos28 18A9_L_rev 18A9_R_for 18A9_R_rev 18A9_fOrf2_for 18A9_fOrf2_rev 18A9_fOrf4_for 18A9_fOrf4_rev Knock_ppz2_F Knock_ppz2_R Knock_ppz3_F Knock_ppz3_R Knock_ppz4_F Knock_ppz4_R Knock_ppzM_F Knock_ppzM_R 18A9_fppz8_F 18A9_fppz8_R 18A9_fOrf5_F 18A9_fOrf5_R 5 -CTA CGC GGT GGC CGC CGC CGC GTC CGC CGC CCG GGC CTT ACT AGT TGT AGG CTG GAG CTG CTT C-3 5 -CGG AAT TCG TTA CTC CAG TCG CGT GCG TGT GTC GTC GGT TCT AGA ATT CCG GGG ATC CGT CGA CC-3 5 -TTC GGC TGA GCT GAA AGA TAT CTG CGA AAA CTT TCG ATG ACT AGT TGT AGG CTG GAG CTG CTT C-3 5 -GCC AAG CAG GGC AAG CCC GCC CAC GAG TTC TCG GTG AAG TCT AGA ATT CCG GGG ATC CGT CGA CC-3 5 -CCG GAG CCG GAC ACC GCC TGG TAG GAG GCG ACG GTC AGC ACT AGT TGT AGG CTG GAG CTG CTT C-3 5 -CGG AAT TCG TTA CTC CAG TCG CGT GCG TGT GTC GTC GGT TCT AGA ATT CCG GGG ATC CGT CGA CC-3 5 -CGC CAT GAT CGG GTG CGT GGA GGG CAG CTG GTC CTG CCG ACT AGT TGT AGG CTG GAG CTG CTT C-3 5 -CCC CGT TTT CCC GTT CAC TTC TTC CCA GAG GGG GGC ATG TCT AGA ATT CCG GGG ATC CGT CGA CC-3 5 -GCG CCA CAG GCG CCG GCC CCG GTG TCG TCT TGA CGG TCT ACT AGT TGT AGG CTG GAG CTG CTT C-3 5 -GGC CGG CGC CTG TGG CGC CGG CCC CGG CCG AGA CCG ATG TCT AGA ATT CCG GGG ATC CGT CGA CC-3 5 -ACG GCA CTC CGG GCC GCT CGG AGT TCG ACA TAG AGG AAC ACT AGT TGT AGG CTG GAG CTG CTT C-3 5 -CAT CAC TGG AAC AGG TCA TCT CAT GAA GGA GAC TCC ATG TCT AGA ATT CCG GGG ATC CGT CGA CC-3 5 -CCA CGT TCG TCG GCA CGA GTC TGT GGC GGG GCA GCC TGG ACT AGT TGT AGG CTG GAG CTG CTT C-3 5 -CCC GCC CGC CCG CAC CCG CCC ATG AGG AGA CAG GCC ATG TCT AGA ATT CCG GGG ATC CGT CGA CC-3 5 -GGC GGG AGG ATC CGG TCG CCG GCC GTC GGC ACG TCA TGG ACT AGT TGT AGG CTG GAG CTG CTT C-3 5 -CCT CAC CGA CTT CCA AGC GCT GCT CAC CAA CTC CAG CGC TCT AGA ATT CCG GGG ATC CGT CGA CC-3 5 -TTC GGC TGA GCT GAA AGA TAT CTG CGA AAA CTT TCG ATG ACT AGT TGT AGG CTG GAG CTG CTT C-3 5 -GCA TCA GGC CCG GGT CGT GGG CGA GAT GGC GGA CGC CCA TCT AGA ATT CCG GGG ATC CGT CGA CC-3 5 -TTC GGC TGA GCT GAA AGA TAT CTG CGA AAA CTT TCG ATG ACT AGT TGT AGG CTG GAG CTG CTT C-3 S8
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