BIOENERGETICS. 1. Detection of succinate dehydrogenase activity in liver homogenate using artificial electron acceptors.
|
|
- Leon Banks
- 5 years ago
- Views:
Transcription
1 BIOENERGETICS Problems to be prepared: 1. Methods of enzymes activity assessment, the role of artificial electron acceptors and donors. 2. Reactions catalyzed by malate dehydrogenase, succinate dehydrogenase, cytochrome oxidase, lactate dehydrogenase and cellular localization of these enzymes. 3. Processes supplied cell in energy. 1. Detection of succinate dehydrogenase activity in liver homogenate using artificial electron acceptors. Reaction catalyzed by succinate dehydrogenase: CH 2 CH + FAD + FADH 2 CH 2 HC Succinate dehydrogenase is a flavoprotein catalyzing reversible conversion (oxidation) of succinate to fumarate (see figure above). It is the only enzyme of Krebs cycle bound to the inner mitochondrial membrane. It is also the component of protein complex named succinate-ubichinon reductase, the element of electron transport chain. Using this complex, electrons and protons from FADH 2 linked with succinate dehydrogenase are transferred onto respiratory chain. colored (blue) oxidized form colorless reduced form Ryc.1. Reaction of methylene blue reduction. Principle of the method Two artificial electron acceptors are used for detection of succinate dehydrogenase activity: methylene blue and dichlorophenoloindophenol (DCPI). Both are colored in oxidized form and colorless in reduced state. Standard red-ox potential for methylene blue is V, and for DCPI is V. Both red-ox potentials are higher than red-ox potential of FAD/FADH 2, which is V. Therefore, protons from FADH 2 can be transferred to either methylene blue or DCPI. The gradual discoloration, which is the effect of methylene blue or DCPI reduction, is the evidence for proceeding the reaction catalyzed by succinate dehydrogenase. Since the reduced form of methylene blue may also be oxidized by molecular oxygen, the in tubes should be covered with a 1
2 liquid paraffin. Two competitive inhibitors of succinate dehydrogenase are used in the experiment: malonate and pyrophosphate. Malonate has a similar structure to that of succinate, and is the most known competitive inhibitor of the tested enzyme. The less known is fact that pyrophosphate, in spite of its different chemical structure, is a competitive inhibitor too. a) methylene blue as an acceptor Materials and chemicals: mol/dm 3 TRIS-HCl 2. 1 mol/dm 3 sodium succinate % methylene blue mol/dm 3 sodium malonate 5. 5% liver homogenate 6. 5% boiled liver homogenate Add chemicals to five tubes according to table 1. Table mol/dm 3 TRIS-HCl mol/dm 3 sodium succinate 0.02 % methylene blue mol/dm 3 sodium 0.2 malonate H 2 O % liver homogenate boiled liver homogenate After addition of homogenate, shake tubes intensively and immediately put one cm layer of liquid paraffin on the top of the tubes content (after covering with paraffin do not shake tubes!) Incubate tubes at 37 C for 1.5 h. Observe color changes in all tubes after 45 min and 1.5 h of incubation. b) DCPI as an acceptor Materials and chemicals: mol/dm 3 TRIS-HCl 2. 1 mol/dm 3 sodium succinate % DCPI mol/dm 3 sodium malonate 5. 5% sodium pyrophosphate mol/dm 3 KCN 7. 1% liver homogenate 2
3 DCPI colored (blue) oxidized form DCPI colorless reduced form Ryc.2. Reaction of DCPI reduction Add chemicals to seven tubes according to table 2. Table mol/dm 3 TRIS-HCl mol/dm 3 sodium succinate 0.008% DCPI % sodium pyrophosphate mol/dm 3 sodium 0.5 malonate 0.1 mol/dm 3 KCN 0.2 H 2 O % liver homogenate boiled liver homogenate After addition of homogenate shake tubes and incubate mixtures at room temperature for 60 min. Observe color changes in all tubes after 30 and 60 min. 2. Detection of cytochrome oxidase activity in heart homogenate Cytochrome oxidase is IV protein complex of electron transport chain. It is a hemoprotein consisted of ten polypeptide chains and comprises two cytochromes in the structure: a and a 3. Each cytochrome contains Cu + and Fe 2+ ions. Cytochrome oxidase is the last component of electron transport chain and catalyzes cytochrom c oxidation with direct reduction of oxygen molecule. By the action of cytochrome oxidase, O 2 is reduced at the cost of 2Fe 2+ and 2Cu + oxidation, following by formation of 2 molecules of H 2 O. 3
4 Reaction catalyzed by cytochrome oxidase: 4 cytochromes c(fe 2+ ) (reduced) + O 2 + 4H + 4 cytochromes c(fe 3+ ) (oxidized) + 2H 2 O Principle of the method P-phenyldiamine is used as an artificial electrons donor. Red-ox potential of p- phenyldiamine is slightly lower than cytochrome c. Thus, when cytochrome oxidase is active and total amount of cytochrom c is sufficient, p-phenyldiamine becomes oxidized in the presence of oxygen. The appearance of a color in the tubes indicates the reaction progress. P-phenyldiamine does not become oxidized without presence of cytochrome oxidase. colorless reduced form colored oxidized form Ryc.3. Reaction of p-phenyldiamine oxidation. Materials and chemicals mol/dm 3 Tris-HCl % cytochrome c mol/dm 3 KCN 4. 1% p-phenyldiamine 5. 5% heart homogenate 6. 5% boiled heart homogenate Add chemicals to six tubes according to table 3. Table mol/dm 3 Tris-HCl % cytochrome c mol/dm 3 KCN 3 drops H 2 O % p-phenyldiamine % heart homogenate boiled heart homogenate 4
5 After addition of all chemicals, shake the tubes content and observe color changes over time. Interpret results. For most of micromolecular metabolites the inner mitochondrial membrane has a very limited permeability, which is conditioned by the presence of specific transporters like ADP-ATP translocase, phosphate translocase etc. Outer mitochondrial membrane acts as molecular bolter (riddle) and allows for passing of compounds with molecular weight not exceeding daltons. Thus, exogenous cytochrome c (with molecular weight of daltons) does not pass through intact outer membrane. If a part of mitochondria is damaged by too intensive homogenization, exogenous cytochrome c can react with cytochrome oxidase, which is located in inner mitochondrial membrane next to the outer surface. The increase of cytochrome c concentration leads to the stimulation of cytochrome oxidase activity. If a very small amount of mitochondria from heart homogenate is damaged during homogenization, s in tubes 4 and 6 have a similar color. 3. Localization of succinate dehydrogenase, lactate dehydrogenase and cytochrome oxidase in subcellular fractions of the liver. Reaction catalyzed by lactate dehydrogenase (LDH) H C OH + NAD + C = O + NADH + H + CH 3 CH 3 Succinate dehydrogenase and cytochrome oxidase are found exclusively in mitochondria, whereas lactate dehydrogenase is a cytoplasmatic enzyme, participating in glycolysis. LDH catalyzes reversible reaction of oxidation of lactate to pyruvate. In highly aerobic tissues (oxygenated) e.g. in the liver and heart muscle, lactate absorbed from blood is oxidized to pyruvate, what enables its utilization in gluconeogenesis (in liver) or as energetic fuel (in heart muscle). In the cells with anaerobic metabolism (red blood cell, intensively working skeletal muscle cells) reaction catalyzed by lactate dehydrogenase makes glycolysis possible to proceed under anaerobic conditions. Reduction of pyruvate by LDH enables oxidation of NADH + H + when electron transfer chain cannot run. Thus glycolysis, in contrast to other metabolic pathways, can run and generate ATP by substrate level phosphorylation. LDH belongs to a group of indicatory enzymes. Estimation of its activity and detection of isoenzymes presence in blood serum is used in diagnosis of liver and heart diseases. Materials and chemicals mol/dm 3 Tris-HCl % DCPI 3. 1 mol/dm 3 sodium succinate 4. 1 mol/dm 3 sodium lactate % cytochrome c 6. 1% p-phenyldiamine mmol/dm 3 NAD + (in Eppendorf tubes) mol/dm 3 sodium malonate 9. 5% mitochondria suspension 10. 5% cytosolic fraction 5
6 Add chemicals to nine tubes according to table 4. Table mol/dm 3 Tris-HCl % DCPI mol/dm 3 sodium lactate mol/dm 3 sodium succinate % cytochrome c % p-phenyldiamine mmol/dm 3 NAD H 2 O mol/dm 3 sodium malonate 0.5 5% mitochondria suspension % cytosolic fraction boiled cytosolic fraction Shake the tubes content and observe color changes over time. The positive result in tube 2 may be due to partial degradation of mitochondria during separation and passing the membrane fragments to postmitochondrial supernatant. The positive result in tube 5 may indicate that cytosolic lactate dehydrogenase was absorbed on mitochondrial suspension. 5. Detection of malate dehydrogenase in cytosolic fraction. Effect of ph. Malate dehydrogenase is found in cytosol and mitochondria. In both cellular compartments this enzyme catalyzes the same reaction, but plays different roles. Reaction catalyzed by malate dehydrogenase CHOH CH + NAD + + NADH + H + CH 2 CH 2 In cytosol, malate dehydrogenase participates in transport of hydrogen atoms from NADH + H + of cytosolic dehydrogenases onto respiratory chain (malate/aspartate shuttle). In mitochondria, malate dehydrogenase is one of Krebs cycle enzymes. 6
7 Reaction catalyzed by malate dehydrogenase is strongly endoergic with very low equilibrium constant (K=10-12 ). Equilibrium of this reaction is markedly moved in the direction of malate formation, thus it is necessary to simultaneously remove the reaction products i.e. oxaloacetate and NADH + H +. In vivo in mitochondria oxaloacetate participates in Krebs cycle and NADH is oxidized, transferring protons and electrons onto ETC. In order to show in vitro that oxaloacetate is formed in this reaction, ph must be increased from 7.0 to Under the alkaline conditions, protons (from NADH + H + ) are removed according to the reaction: H + + OH H 2 O and the equilibrium of this reaction shifts into right. Principle of the method Oxaloacetate (produced by malate dehydrogenase) reacts with 2,4- dinitrophenylhydrazine and forms oxaloacetate 2,4-dinitrophenylhydrazone, which is colored under the alkaline conditions. Materials and chemicals mol/dm 3 glycine buffer, ph= mol/dm 3 Tris-HCl buffer, ph = mol/dm 3 Tris-HCl mol/dm 3 sodium malate mmol/dm 3 NAD + (in Eppendorf tubes) % 2,4-dinitrophenylhydrazine mol/dm 3 NaOH 8. 5% cytosolic fraction Add chemicals to four tubes according to table 5. Table mol/dm 3 glycine buffer, ph= mol/dm 3 Tris-HCl buffer, ph= mol/dm 3 Tris-HCl mmol/dm 3 NAD mol/dm 3 sodium malate H 2 O 0.1 5% cytosolic fraction After 30 minutes of incubation at 37 C add 0.6 cm 3 of 0.02% 2,4-dinitrophenylhydrazine to all tubes, and next 2 cm 3 of 1.5 mol/l NaOH. After addition of all chemicals, shake the tubes content and observe color changes over time. Interpret results. 7
How Cells Release Chemical Energy. Chapter 7
How Cells Release Chemical Energy Chapter 7 7.1 Overview of Carbohydrate Breakdown Pathways All organisms (including photoautotrophs) convert chemical energy of organic compounds to chemical energy of
More informationCLASS 11 th. Respiration in Plants
CLASS 11 th 01. Introduction All living cells require continuous supply of energy to perform various vital activities. This energy is released in controlled manner for cellular use via the process of respiration.
More informationIII. 6. Test. Respiració cel lular
III. 6. Test. Respiració cel lular Chapter Questions 1) What is the term for metabolic pathways that release stored energy by breaking down complex molecules? A) anabolic pathways B) catabolic pathways
More informationElectron Transport and Oxidative. Phosphorylation
Electron Transport and Oxidative Phosphorylation Electron-transport chain electron- Definition: The set of proteins and small molecules involved in the orderly sequence of transfer to oxygen within the
More informationElectron transport chain,oxidative phosphorylation & mitochondrial transport systems. M.Kohutiar, B.Sopko
Electron transport chain,oxidative phosphorylation & mitochondrial transport systems. M.Kohutiar, B.Sopko Content 1. Structure of mitochondria Mitochondrial transport systems 2. Electron transport 3. Parts
More informationMULTIPLE CHOICE QUESTIONS
MULTIPLE CHOICE QUESTIONS 1. Which of the following statements concerning anabolic reactions is FALSE? A. They are generally endergonic. B. They usually require ATP. C. They are part of metabolism. D.
More informationElectron transport chain chapter 6 (page 73) BCH 340 lecture 6
Electron transport chain chapter 6 (page 73) BCH 340 lecture 6 The Metabolic Pathway of Cellular Respiration All of the reactions involved in cellular respiration can be grouped into three main stages
More informationName Class Date. 1. Cellular respiration is the process by which the of "food"
Name Class Date Cell Respiration Introduction Cellular respiration is the process by which the chemical energy of "food" molecules is released and partially captured in the form of ATP. Carbohydrates,
More informationVocabulary. Chapter 20: Electron Transport and Oxidative Phosphorylation
Vocabulary ATP Synthase: the enzyme responsible for production of ATP in mitochondria Chemiosmotic Coupling: the mechanism for coupling electron transport to oxidative phosphorylation; it requires a proton
More informationRespiration. Respiration. Respiration. How Cells Harvest Energy. Chapter 7
How Cells Harvest Energy Chapter 7 Organisms can be classified based on how they obtain energy: autotrophs: are able to produce their own organic molecules through photosynthesis heterotrophs: live on
More informationHow Cells Harvest Energy. Chapter 7. Respiration
How Cells Harvest Energy Chapter 7 Respiration Organisms classified on how they obtain energy: autotrophs: produce their own organic molecules through photosynthesis heterotrophs: live on organic compounds
More informationCarbohydrate Metabolism by Yeast Experiment #12
Carbohydrate Metabolism by Yeast Experiment #12 Objective: To observe enzyme activities in respiring yeast by observing color changes in methylene blue dye as a substitute for NAD + in the yeast respiratory
More informationRespiration. Respiration. How Cells Harvest Energy. Chapter 7
How Cells Harvest Energy Chapter 7 Respiration Organisms can be classified based on how they obtain energy: autotrophs: are able to produce their own organic molecules through photosynthesis heterotrophs:
More information2/4/17. Cellular Metabolism. Metabolism. Cellular Metabolism. Consists of all of the chemical reactions that take place in a cell.
Metabolism Cellular Metabolism Consists of all of the chemical reactions that take place in a cell. Can be reactions that break things down. (Catabolism) Or reactions that build things up. (Anabolism)
More informationMarah Bitar. Faisal Nimri ... Nafeth Abu Tarboosh
8 Marah Bitar Faisal Nimri... Nafeth Abu Tarboosh Summary of the 8 steps of citric acid cycle Step 1. Acetyl CoA joins with a four-carbon molecule, oxaloacetate, releasing the CoA group and forming a six-carbon
More informationOxidative Phosphorylation
Electron Transport Chain (overview) The NADH and FADH 2, formed during glycolysis, β- oxidation and the TCA cycle, give up their electrons to reduce molecular O 2 to H 2 O. Electron transfer occurs through
More informationCellular Respiration Stage 2 & 3. Glycolysis is only the start. Cellular respiration. Oxidation of Pyruvate Krebs Cycle.
Cellular Respiration Stage 2 & 3 Oxidation of Pyruvate Krebs Cycle AP 2006-2007 Biology Glycolysis is only the start Glycolysis glucose pyruvate 6C 2x 3C Pyruvate has more energy to yield 3 more C to strip
More informationCellular Metabolism. Biol 105 Lecture 6 Read Chapter 3 (pages 63 69)
Cellular Metabolism Biol 105 Lecture 6 Read Chapter 3 (pages 63 69) Metabolism Consists of all of the chemical reactions that take place in a cell Metabolism Animation Breaking Down Glucose For Energy
More informationChapter 9 Cellular Respiration Overview: Life Is Work Living cells require energy from outside sources
Chapter 9 Cellular Respiration Overview: Life Is Work Living cells require energy from outside sources Some animals, such as the giant panda, obtain energy by eating plants, and some animals feed on other
More information4. Which step shows a split of one molecule into two smaller molecules? a. 2. d. 5
1. Which of the following statements about NAD + is false? a. NAD + is reduced to NADH during both glycolysis and the citric acid cycle. b. NAD + has more chemical energy than NADH. c. NAD + is reduced
More informationCellular Metabolism 6/20/2015. Metabolism. Summary of Cellular Respiration. Consists of all the chemical reactions that take place in a cell!
Cellular Metabolism Biology 105 Lecture 6 Chapter 3 (pages 56-61) Metabolism Consists of all the chemical reactions that take place in a cell! Cellular metabolism: Aerobic cellular respiration requires
More informationBY: RASAQ NURUDEEN OLAJIDE
BY: RASAQ NURUDEEN OLAJIDE LECTURE CONTENT INTRODUCTION CITRIC ACID CYCLE (T.C.A) PRODUCTION OF ACETYL CoA REACTIONS OF THE CITIRC ACID CYCLE THE AMPHIBOLIC NATURE OF THE T.C.A CYCLE THE GLYOXYLATE CYCLE
More informationChemical Energy. Valencia College
9 Pathways that Harvest Chemical Energy Valencia College 9 Pathways that Harvest Chemical Energy Chapter objectives: How Does Glucose Oxidation Release Chemical Energy? What Are the Aerobic Pathways of
More informationCellular Metabolism 9/24/2013. Metabolism. Cellular Metabolism. Consists of all the chemical reactions that take place in a cell!
Cellular Metabolism Biology 105 Lecture 6 Chapter 3 (pages 56-61) Metabolism Consists of all the chemical reactions that take place in a cell! Cellular Metabolism Aerobic cellular respiration requires
More informationCellular Metabolism. Biology 105 Lecture 6 Chapter 3 (pages 56-61)
Cellular Metabolism Biology 105 Lecture 6 Chapter 3 (pages 56-61) Metabolism Consists of all the chemical reactions that take place in a cell! Cellular Metabolism Aerobic cellular respiration requires
More informationBIOLOGY - CLUTCH CH.9 - RESPIRATION.
!! www.clutchprep.com CONCEPT: REDOX REACTIONS Redox reaction a chemical reaction that involves the transfer of electrons from one atom to another Oxidation loss of electrons Reduction gain of electrons
More informationCellular Respiration: Harvesting Chemical Energy Chapter 9
Cellular Respiration: Harvesting Chemical Energy Chapter 9 Assemble polymers, pump substances across membranes, move and reproduce The giant panda Obtains energy for its cells by eating plants which get
More informationTCA CYCLE (Citric Acid Cycle)
TCA CYCLE (Citric Acid Cycle) TCA CYCLE The Citric Acid Cycle is also known as: Kreb s cycle Sir Hans Krebs Nobel prize, 1953 TCA (tricarboxylic acid) cycle The citric acid cycle requires aerobic conditions!!!!
More informationSection B: The Process of Cellular Respiration
CHAPTER 9 CELLULAR RESPIRATION: HARVESTING CHEMICAL ENERGY Section B: The Process of Cellular Respiration 1. Respiration involves glycolysis, the Krebs cycle, and electron transport: an overview 2. Glycolysis
More informationCellular Pathways That Harvest Chemical Energy. Cellular Pathways That Harvest Chemical Energy. Cellular Pathways In General
Cellular Pathways That Harvest Chemical Energy A. Obtaining Energy and Electrons from Glucose Lecture Series 12 Cellular Pathways That Harvest Chemical Energy B. An Overview: Releasing Energy from Glucose
More informationCh. 9 Cell Respiration. Title: Oct 15 3:24 PM (1 of 53)
Ch. 9 Cell Respiration Title: Oct 15 3:24 PM (1 of 53) Essential question: How do cells use stored chemical energy in organic molecules and to generate ATP? Title: Oct 15 3:28 PM (2 of 53) Title: Oct 19
More informationA) Choose the correct answer: 1) Reduction of a substance can mostly occur in the living cells by:
Code: 1 1) Reduction of a substance can mostly occur in the living cells by: (a) Addition of oxygen (b) Removal of electrons (c) Addition of electrons (d) Addition of hydrogen 2) Starting with succinate
More informationOxidative phosphorylation & Photophosphorylation
Oxidative phosphorylation & Photophosphorylation Oxidative phosphorylation is the last step in the formation of energy-yielding metabolism in aerobic organisms. All oxidative steps in the degradation of
More information3. Distinguish between aerobic and anaerobic in terms of cell respiration. Outline the general process of both.
3.7 Cell Respiration 1. Define cell respiration. Cell respiration is the controlled release of energy from organic molecules in cells to form ATP. 2. State the equation for the process of cell respiration.
More informationHigher Biology. Unit 2: Metabolism and Survival Topic 2: Respiration. Page 1 of 25
Higher Biology Unit 2: Metabolism and Survival Topic 2: Respiration Page 1 of 25 Sub Topic: Respiration I can state that: All living cells carry out respiration. ATP is the energy currency of the cell
More informationReleasing Chemical Energy
Releasing Chemical Energy Ø Energy From Carbohydrates Ø Aerobic Respiration/ Stages Ø Fermentation Ø Food as a Source of Energy How Do Cells Access the Chemical Energy in Carbohydrayes? Aerobic Respiration
More informationChapter 5. Microbial Metabolism
Chapter 5 Microbial Metabolism Metabolism Collection of controlled biochemical reactions that take place within a microbe Ultimate function of metabolism is to reproduce the organism Metabolic Processes
More informationOxidative Phosphorylation
Oxidative Phosphorylation Energy from Reduced Fuels Is Used to Synthesize ATP in Animals Carbohydrates, lipids, and amino acids are the main reduced fuels for the cell. Electrons from reduced fuels are
More informationBIO 311C Spring Lecture 27 Monday 5 Apr. 1
BIO 311C Spring 2010 Lecture 27 Monday 5 Apr. 1 Review Metabolic Pathways and Processes that Participate in Respiration - Glycolysis Occurs in the cytoplasmic matrix - Pyruvate dehydrogenase - Krebs Cycle
More informationChapter 9. Cellular Respiration and Fermentation
Chapter 9 Cellular Respiration and Fermentation Energy flows into an ecosystem as sunlight and leaves as heat Photosynthesis generates O 2 and organic molecules, which are used in cellular respiration
More informationRESPIRATION Worksheet
A.P. Bio L.C. RESPIRATION Worksheet 1. In the conversion of glucose and oxygen to carbon dioxide and water a) which molecule becomes reduced? b) which molecule becomes oxidized? c) what happens to the
More informationElectron Transport and oxidative phosphorylation (ATP Synthesis) Dr. Howaida Nounou Biochemistry department Sciences college
Electron Transport and oxidative phosphorylation (ATP Synthesis) Dr. Howaida Nounou Biochemistry department Sciences college The Metabolic Pathway of Cellular Respiration All of the reactions involved
More informationChapter 9. Cellular Respiration: Harvesting Chemical Energy
Chapter 9 Cellular Respiration: Harvesting Chemical Energy Living cells require energy from outside sources Energy flows into an ecosystem as sunlight and leaves as heat Photosynthesis generates O 2 and
More information1 Which pathway for aerobic cellular respiration is located in the cytoplasm of the cell?
1 Which pathway for aerobic cellular respiration is located in the cytoplasm of the cell? glycolysis citric cycle 2 Which of the following statements is NOT correct regarding aerobic cellular respiration?
More informationChapter 8 Mitochondria and Cellular Respiration
Chapter 8 Mitochondria and Cellular Respiration Cellular respiration is the process of oxidizing food molecules, like glucose, to carbon dioxide and water. The energy released is trapped in the form of
More information2
1 2 3 4 5 6 7 8 9 10 11 What is the fate of Pyruvate? Stages of Cellular Respiration GLYCOLYSIS PYRUVATE OX. KREBS CYCLE ETC 2 The Krebs Cycle does your head suddenly hurt? 3 The Krebs Cycle An Overview
More informationRespiration. Organisms can be classified based on how they obtain energy: Autotrophs
Respiration rganisms can be classified based on how they obtain energy: Autotrophs Able to produce their own organic molecules through photosynthesis Heterotrophs Live on organic compounds produced by
More informationCell Respiration Assignment Score. Name Sec.. Date.
Cell Respiration Assignment Score. Name Sec.. Date. Working by alone or in a group, answer the following questions about Cell Respiration. This assignment is worth 30 points with the possible points for
More informationObjective: You will be able to construct an explanation for how each phase of respiration captures and stores free energy.
Objective: You will be able to construct an explanation for how each phase of respiration captures and stores free energy. Do Now: Compare and contrast the three black equations below ADP + P + Energy
More informationAEROBIC RESPIRATION. Chapter 8
AEROBIC RESPIRATION Chapter 8 AEROBIC RESPIRATION Aerobic respiration is the next step after Glycolysis if the cell can obtain oxygen. We won t need it until the last step but we still need it. Remember
More informationTrue or False: 1. Reactions are called endergonic if they occur spontaneously and release free energy.
True or False: 1. Reactions are called endergonic if they occur spontaneously and release free energy. 2. Enzymes catalyze chemical reactions by lowering the activation energy 3. Biochemical pathways are
More informationCellular Respiration
Cellular Respiration C 6 H 12 O 6 + 6O 2 -----> 6CO 2 + 6H 2 0 + energy (heat and ATP) 1. Energy Capacity to move or change matter Forms of energy are important to life include Chemical, radiant (heat
More informationCitric acid cycle and respiratory chain. Pavla Balínová
Citric acid cycle and respiratory chain Pavla Balínová Mitochondria Structure of mitochondria: Outer membrane Inner membrane (folded) Matrix space (mtdna, ribosomes, enzymes of CAC, β-oxidation of FA,
More informationChapter 9: Cellular Respiration Overview: Life Is Work. Living cells. Require transfusions of energy from outside sources to perform their many tasks
Chapter 9: Cellular Respiration Overview: Life Is Work Living cells Require transfusions of energy from outside sources to perform their many tasks Biology, 7 th Edition Neil Campbell and Jane Reece The
More informationBIL 256 Cell and Molecular Biology Lab Spring, Tissue-Specific Isoenzymes
BIL 256 Cell and Molecular Biology Lab Spring, 2007 Background Information Tissue-Specific Isoenzymes A. BIOCHEMISTRY The basic pattern of glucose oxidation is outlined in Figure 3-1. Glucose is split
More information3.7.1 Define cell respiration [Cell respiration is the controlled release of energy from organic compounds in cells to form ATP]
3.7 Cell respiration ( Chapter 9 in Campbell's book) 3.7.1 Define cell respiration [Cell respiration is the controlled release of energy from organic compounds in cells to form ATP] Organic compounds store
More informationWHY IS THIS IMPORTANT?
CHAPTER 3 ESSENTIALS OF METABOLISM WHY IS THIS IMPORTANT? It is important to have a basic understanding of metabolism because it governs the survival and growth of microorganisms The growth of microorganisms
More informationMULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question.
Respiration Practice Name MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 1) Which of the following statements describes NAD+? A) NAD+ can donate
More informationOVERVIEW OF RESPIRATION AND LOOSE ENDS. What agents? What war?
5.19.06 OVERVIEW OF RESPIRATION AND LOOSE ENDS What agents? What war? 1 Ubiquinone or Coenzyme Q: small hydrophobic molecule that can pick up or donate electrons The respiratory chain contains 3 large
More informationBackground knowledge
Background knowledge This is the required background knowledge: State three uses of energy in living things Give an example of an energy conversion in a living organism State that fats and oils contain
More informationCELL BIOLOGY - CLUTCH CH AEROBIC RESPIRATION.
!! www.clutchprep.com CONCEPT: OVERVIEW OF AEROBIC RESPIRATION Cellular respiration is a series of reactions involving electron transfers to breakdown molecules for (ATP) 1. Glycolytic pathway: Glycolysis
More informationCELLULAR RESPIRATION. Xe - + Y X + Ye - CH 4 + 2O 2 CO 2 + H 2 O + energy. C 6 H 12 O 6 + 6O 2 6CO 2 + 6H 2 O + energy SUMMARY EQUATION
AP BIOLOGY CELLULAR ENERGETICS ACTIVITY #2 NAME DATE HOUR CELLULAR RESPIRATION SUMMARY EQUATION STEPWISE REDOX REACTION Oxidation: Reduction: Xe - + Y X + Ye - CH 4 + 2O 2 CO 2 + H 2 O + energy C 6 H 12
More information3.7 CELLULAR RESPIRATION. How are these two images related?
3.7 CELLULAR RESPIRATION How are these two images related? CELLULAR RESPIRATION Cellular respiration is the process whereby the body converts the energy that we get from food (glucose) into an energy form
More informationNotes CELLULAR RESPIRATION SUMMARY EQUATION C 6 H 12 O 6 + O 2. 6CO 2 + 6H 2 O + energy (ATP) STEPWISE REDOX REACTION
AP BIOLOGY CELLULAR ENERGETICS ACTIVITY #2 Notes NAME DATE HOUR SUMMARY EQUATION CELLULAR RESPIRATION C 6 H 12 O 6 + O 2 6CO 2 + 6H 2 O + energy (ATP) STEPWISE REDOX REACTION Oxidation: partial or complete
More informationEnzymes and Metabolism
PowerPoint Lecture Slides prepared by Vince Austin, University of Kentucky Enzymes and Metabolism Human Anatomy & Physiology, Sixth Edition Elaine N. Marieb 1 Protein Macromolecules composed of combinations
More informationRespiration. Energy is everything!
Respiration Energy is everything! Tesla was incredible Everyone was intrigued by Tesla Tesla showed that energy does not need to be feared So what does this have to do with twinkies? Everything! Cellular
More informationNotes CELLULAR RESPIRATION SUMMARY EQUATION C 6 H 12 O 6 + O 2. 6CO 2 + 6H 2 O + energy (ATP) STEPWISE REDOX REACTION
AP BIOLOGY CELLULAR ENERGETICS ACTIVITY #2 Notes NAME DATE HOUR SUMMARY EQUATION CELLULAR RESPIRATION C 6 H 12 O 6 + O 2 6CO 2 + 6H 2 O + energy (ATP) STEPWISE REDOX REACTION Oxidation: partial or complete
More informationNOTES: Ch 9 Cellular Respiration: Harvesting Chemical Energy Part 1: The Overview
NOTES: Ch 9 Cellular Respiration: Harvesting Chemical Energy Part 1: The Overview Overview: Life Is Work Living cells require energy from outside sources Some animals, such as the giant panda, obtain energy
More informationCITRIC ACID CYCLE ERT106 BIOCHEMISTRY SEM /19 BY: MOHAMAD FAHRURRAZI TOMPANG
CITRIC ACID CYCLE ERT106 BIOCHEMISTRY SEM 1 2018/19 BY: MOHAMAD FAHRURRAZI TOMPANG Chapter Outline (19-1) The central role of the citric acid cycle in metabolism (19-2) The overall pathway of the citric
More informationChapter 7 Cellular Respiration and Fermentation*
Chapter 7 Cellular Respiration and Fermentation* *Lecture notes are to be used as a study guide only and do not represent the comprehensive information you will need to know for the exams. Life Is Work
More informationBIOLOGY. Cellular Respiration and Fermentation CAMPBELL. Reece Urry Cain Wasserman Minorsky Jackson
CAMPBELL BIOLOGY TENTH EDITION Reece Urry Cain Wasserman Minorsky Jackson 9 Cellular Respiration and Fermentation Lecture Presentation by Nicole Tunbridge and Kathleen Fitzpatrick Figure 9.2 Light energy
More informationCell Respiration. Anaerobic & Aerobic Respiration
Cell Respiration Anaerobic & Aerobic Respiration Understandings/Objectives 2.8.U1: Cell respiration is the controlled release of energy from organic compounds to produce ATP. Define cell respiration State
More informationChapter 9: Cellular Respiration: Harvesting Chemical Energy
AP Biology Reading Guide Name: Date: Period Chapter 9: Cellular Respiration: Harvesting Chemical Energy Overview: Before getting involved with the details of cellular respiration and photosynthesis, take
More informationCh. 9 Cellular Respiration Stage 2 & 3: Oxidation of Pyruvate Krebs Cycle
Ch. 9 Cellular Respiration Stage 2 & 3: Oxidation of Pyruvate Krebs Cycle 2006-2007 Glycolysis is only the start Glycolysis glucose pyruvate 6C Pyruvate has more energy to yield 3 more C to strip off (to
More informationElectron Transport Chain and Oxidative Phosphorylation 20-1
Electron Transport Chain and Oxidative Phosphorylation 20-1 Learning Objectives 1. What Role Does Electron Transport Play in Met.? 2. What Are the Reduction Potentials for the Electron Transport Chain?
More informationMITOCHONDRIA LECTURES OVERVIEW
1 MITOCHONDRIA LECTURES OVERVIEW A. MITOCHONDRIA LECTURES OVERVIEW Mitochondrial Structure The arrangement of membranes: distinct inner and outer membranes, The location of ATPase, DNA and ribosomes The
More informationOxidative phosphorylation
OXIDATIVE PHOSPHORYLATION Oxidative phosphorylation Oxidative reaction Coupled by phosphorylation to the generation of high energy intermediate (ATP or other high phosphagen) Oxidative phosphorylation
More informationCellular Respiration
Cellular I can describe cellular respiration Cellular respiration is a series of metabolic pathways releasing energy from a foodstuff e.g. glucose. This yields energy in the form of ATP adenosine P i P
More informationCellular Respiration and Fermentation
CAMPBELL BIOLOGY IN FOCUS URRY CAIN WASSERMAN MINORSKY REECE 7 Cellular Respiration and Fermentation Lecture Presentations by Kathleen Fitzpatrick and Nicole Tunbridge, Simon Fraser University SECOND EDITION
More informationUnit 2: Metabolic Processes
How is energy obtained biologically? Recall: Red Ox Reactions Unit 2: Metabolic Processes Oxidation Is the chief mechanism by which chemical potential energy is released This energy comes from reduced
More information2. What are the products of cellular respiration? Include all forms of energy that are products.
Name Per Cellular Respiration An Overview Why Respire Anyhoo? Because bucko all cells need usable chemical energy to do work. The methods cells use to convert glucose into ATP vary depending on the availability
More informationMULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question.
2004 BCOR 11 Exam 2 Name: Section: Please note that the chapters covered in this exam 2 (2004) are not the same chapters we are covering this year (2005). That means that you won't be getting more questions
More informationCellular Respiration: Harvesting Chemical Energy
Chapter 9 Cellular Respiration: Harvesting Chemical Energy You should be able to: 1. Explain how redox reactions are involved in energy exchanges. Name and describe the three stages of cellular respiration;
More informationCellular Respiration and Fermentation
CAMPBELL BIOLOGY IN FOCUS URRY CAIN WASSERMAN MINORSKY REECE 7 Cellular Respiration and Fermentation Lecture Presentations by Kathleen Fitzpatrick and Nicole Tunbridge, Simon Fraser University SECOND EDITION
More informationCellular Respiration Harvesting Chemical Energy ATP
Cellular Respiration Harvesting Chemical Energy ATP 2006-2007 What s the point? The point is to make ATP! ATP 2006-2007 Harvesting stored energy Energy is stored in organic molecules carbohydrates, fats,
More informationNAME KEY ID # EXAM 3a BIOC 460. Wednesday April 10, Please include your name and ID# on each page. Limit your answers to the space provided!
EXAM 3a BIOC 460 Wednesday April 10, 2002 Please include your name and ID# on each page. Limit your answers to the space provided! 1 1. (5 pts.) Define the term energy charge: Energy charge refers to the
More informationCellular Respiration- -conversion of stored energy in glucose to usable energy for the cell -energy in cells is stored in the form of ATP
Cellular Respiration Notes Chapter 7 How Cells Make ATP Energy Releasing Pathways Cellular Respiration- -conversion of stored energy in glucose to usable energy for the cell -energy in cells is stored
More informationCELLULAR RESPIRATION SUMMARY EQUATION. C 6 H 12 O 6 + O 2 6CO2 + 6H 2 O + energy (ATP) STEPWISE REDOX REACTION
CELLULAR RESPIRATION SUMMARY EQUATION C 6 H 12 O 6 + O 2 6CO2 + 6H 2 O + energy (ATP) STEPWISE REDOX REACTION Oxidation: partial or complete loss of electrons Reduction: partial or complete gain of electrons
More informationFREE ENERGY Reactions involving free energy: 1. Exergonic 2. Endergonic
BIOENERGETICS FREE ENERGY It is the portion of the total energy change in a system that is available for doing work at constant temperature and pressure; it is represented as ΔG. Reactions involving free
More informationCellular Respiration Harvesting Chemical Energy ATP
Cellular Respiration Harvesting Chemical Energy ATP 2006-2007 What s the point? The point is to make ATP! ATP 2006-2007 Harvesting stored energy Energy is stored in organic molecules carbohydrates, fats,
More informationEnergetics of carbohydrate and lipid metabolism
Energetics of carbohydrate and lipid metabolism 1 Metabolism: The sum of all the chemical transformations taking place in a cell or organism, occurs through a series of enzymecatalyzed reactions that constitute
More informationRespiration. Energy is everything!
Respiration Energy is everything! Tesla was incredible Everyone was intrigued by Tesla Tesla showed that energy does not need to be feared So what does this have to do with twinkies? Everything! Cellular
More informationChapter Seven (Cellular Respiration)
Chapter Seven (Cellular Respiration) 1 SECTION ONE: GLYCOLYSIS AND FERMENTATION HARVESTING CHEMICAL ENERGY Cellular respiration is the process in which cells make adenosine triphosphate (ATP) by breaking
More informationWHY DO WE NEED TO BE ABLE TO RESPIRE?
WHY DO WE NEED TO BE ABLE TO RESIRE? To produce energy AT. We need energy to eat, breathe, move, stay warm etc. THE THREE MAIN ROCESSES IN RESIRATION 1. Glycolysis The first step in respiration 2. Krebs
More informationCitric Acid Cycle: Central Role in Catabolism. Entry of Pyruvate into the TCA cycle
Citric Acid Cycle: Central Role in Catabolism Stage II of catabolism involves the conversion of carbohydrates, fats and aminoacids into acetylcoa In aerobic organisms, citric acid cycle makes up the final
More informationMetabolism. Metabolism. Energy. Metabolism. Energy. Energy 5/22/2016
5//016 Metabolism Metabolism All the biochemical reactions occurring in the body Generating, storing and expending energy ATP Supports body activities Assists in constructing new tissue Metabolism Two
More informationCampbell Biology 9. Chapter 9 Cellular Respiration and Fermentation. Chul-Su Yang, Ph.D., Lecture on General Biology 1
Lecture on General Biology 1 Campbell Biology 9 th edition Chapter 9 Cellular Respiration and Fermentation Chul-Su Yang, Ph.D., chulsuyang@hanyang.ac.kr Infection Biology Lab., Dept. of Molecular & Life
More informationElectron transport chain, oxidative phosphorylation, mitochondrial transport systems
Electron transport chain, oxidative phosphorylation, mitochondrial transport systems JAN ILLNER Respiratory chain & oxidative phosphorylation INTERMEMBRANE SPACE ubiquinone cytochrome c ATPase Production
More informationBIOLOGY 101. CHAPTER 9: Cellular Respiration - Fermentation: Life is Work
BIOLOGY 101 CHAPTER 9: Cellular Respiration - Fermentation: Life is Work An Introduction to Metabolism: Energy of Life 8.3 ATP powers cellular work by coupling exergonic reactions to endergonic reactions
More informationConsists of all of the chemical reactions that take place in a cell. Summary of Cellular Respiration. Electrons transferred. Cytoplasm Blood vessel
7/19/2014 Metabolism Cellular Metabolism Metabolism Consists of all of the chemical reactions that take place in a cell PLAY Animation Breaking Down Glucose For Energy Biol 105 Lecture Packet 6 Read Chapter
More information