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1 Supplementary Table 1. Carbohydrate antigens adopted from the late Prof. Elvin A. Kabat that were utilized in Figure 3 Antigen name Class a Antigen Array ID no. position Description Laboratory or commercial origins References Klebsiella K7 1 1 A1 Klebsiella type 7 From the late Prof. Michael Heidelberger & Nimmich, Immunochemistry 13, 67 (1976) Klebsiella K A2 Klebsiella type 11 From the late Prof. Michael Thurow et al., Carbohydr. Res. 41, 241 (1975) Klebsiella K A3 Klebsiella type 13 From Dr. Morten Harboe (University Neimann et al., Carbohydr. Res. 59, 165 (1977); Neimann et al., capsular of Oslo, Norway). Carbohydr. Res. 60, 353 (1978) Klebsiella K A4 Klebsiella type 21 From the late Prof. Michael Choy & Dutton, Can. J. Chem. 51, 198 (1973) Dudman's Dudman's Rhizobium From the late Prof. Michael Chaudhari et al., Carbohydr. Rhizobium tritolii 1 5 A5 tritolii TA1 Heidelberger (NY University) Res. 28, 221 (1973) TA1 Hoffman et al., Fed. Proc., Fed. Chondroitin Am. Soc. Exp. Biol. 17, A6 Chondroitin sulfate B Sigma Chemical Co., St. Louis, MO sulfate "B" (1958); Kabat et al., Carbohydr. Res. 130, 289 (1984)

2 Pneumococcal C From the late Prof. Sam M. Beiser Pneumococcal C 1 7 C1 (Columbia University) (species-specific) type VIII From the late Prof. Michael 1 8 C2 type VIII type XIV 1 9 C3 type XIV capsular Cow blood group B Cow 21 (blood substance, phenol 3 10 C4 group B) insoluble, 10% precipitations (ppt) Bacto-agar 20 C Bacto-agar 20 C 1 11 C5 extract extract Arabino galactan Arabino galactan 1 12 C6 (Larch) (Larch CORASH) From Mr. T.E. Gilmore of E.R. Squibb and Son, No. 227, Lot gastric mucin derived from stomach linings of cow no. 21 by phenol extraction and fractional ethanol precipitation (10% ppt) Bacto-agar (Difco Laboratories, Detroit, MI), treated and extracted in Kabat's laboratory (Jerry Liao). The fraction is soluble at 20 C From Dr. Niel Glaudemans (NIH, Bethesda, MD) IM3-BSA 4 13 E1 Isomaltotriose-BSA From Dr. D. Zopf (NCI, NIH) Kabat et al., J. Exp. Med. 152, 979 (1980) Brown, R., J. Immunol. 37, 445 (1939) Howe et al., J. Am. Chem. Soc. 80, 6656 (1958) Beiser & Kabat, J. Immunol. 68, (1952) Duckworth & Yaphe, Carbohydr. Res. 16, 189 (1971) Kabat et al., J. Exp. Med. 152, 979 (1980) Zopf et al., Methods Enzymol. 50, 163 (1978); Smith et al., Methods Enzymol. 50, 169 (1978)

3 IM3-KLH 4 14 E2 Isomaltotriose-KLH From Dr. D. Zopf (NCI, NIH) Blood group substance human cyst fluid by pepsin digestion Le a (N-1 10% 2 ) 3 15 E3 Lewis a. N-1 10% ppt and ethanol ppt (10% ethanol, 2 ). 2 The cyst fluid of a Nigerian patient was provided by Ada E. Bezer (WHO) human ovarian cyst fluid. The Beach P1 fractions were the non-dialyzable Human ovarian cyst portion of the blood group B Beach P1 (blood blood group B 3 16 E4 substance after hydrolysis at ph 1.5 2, group B) substance, Beach P1 which removes most of the α-l fraction fucosyl end groups but retains blood group B active oligosaccharide side chains Human ovarian cyst Tij II, 20% from blood group B and Le a human ovarian cyst fluid of a Nigerian 2nd 10% (blood 3 17 E5 substances, Tij II, patient (Tij) provided by Ada E. Bezer group B and Le a ) fraction of 20% from (WHO). 2nd 10% Zopf et al., Methods Enzymol. 50, 163 (1978); Smith et al., Methods Enzymol. 50, 169 (1978) Lloyd et al., Biochemistry 7, 2976 (1968) Allen & Kabat, J. Immunol. 82, (1959); Schiffman et al., J. Am. Chem. Soc. 82, 1122 (1959). Maisonrouge-McAuliffe & Kabat, Arch. Biochem. Biophys. 175, (1976) (Please see Fig.1 for the fractions of isolation and purification).

4 2nd 10% KABAT laboratory, prepared from human ovarian cyst fluid by two OG Blood group substance precipitations with 10% ethanol. The 3 18 E6 (blood group I) I. 10% ppt 2 ovarian cyst fluid of a Nigerian patient (OG) was kindly provided by Ada E. Bezer (WHO) KABAT Laboratory, prepared by Gilbert Ashwell (NIH). Preparation of ASOR 3 19 G1 Asialo-orosomucoid orosomucoid was from Dr. Sten Hammarstrom (University of Stockholm, Sweden) LNT-BSA 4 20 G2 Lacto-N-tetraose BSA From Dr. D. Zopf (NCI, NIH) Phosphomannan 1 21 G3 Phosphomannan, Northern Regional research laboratory NRRL B-2448 (Peoria, IL) NRRL B-2448 Meningococcus group Meningococcus From Dr. Carlos A. Moreno (Kings 1 22 G4 B capsular group B College, London) Haemophilus H. influenzae capsular From Drs. John Robbins and Rachel 1 23 G5 influenzae type A type A Schneerson (NICHD, NIH) Vicari & Kabat, J. Immunol. 102, 821 (1969); Feizi et al., J. Exp. Med. 133, 39 (1971) Kawasaki & Ashwell, J. Biol. Chem. 252, (1977) Kabat et al., J. Immunol. 128, 540 (1982) Gotschlich et al., J. Exp. Med. 129, 1239 (1969); Kabat et al., J. Exp. Med. 164,

5 E. coli K92 (strain MT Escherichia coli From Drs. John Robbins and Rachel 1 24 G6 1389) capsular K92 Schneerson (NICHD, NIH) Klebsiella type Klebsiella A3 capsular From Dr. I.W. Sutherland (Edinburgh 1 25 I1 A3 University, Scotland) Klebsiella K I2 Klebsiella type 12 From Dr. Morten Harboe (University capsular of Oslo, Norway). Klebsiella K I3 Klebsiella type 14 From the late Prof. Sam M. Beiser capsular (Columbia University) Klebsiella K I4 Klebsiella type 33 From Dr. Bengt Lindberg (University capsular of Stockholm, Sweden) Chondroitin sulfate "A" 2 29 I5 Chondroitin sulfate A Sigma Chemical Co., St. Louis, MO Chondroitin sulfate "C" 2 30 I6 Chondroitin sulfate C Sigma Chemical Co., St. Louis, MO type IV From the late Prof. Michael 1 31 K1 soluble capsular type SIV Heidelberger type IX From the late Prof. Michael 1 32 K2 type IX type 27 From the late Prof. Michael 1 33 K3 type 27 Dutton & Savage, Carbohydr. Res. 83, 351 (1980) Dutton & Mackie, Carbohydr. Res. 55, 49 (1977) Lindberg et al., Carbohydr. Res. 76, 281 (1979) Kabat et al., Carbohydr. Res. 130, 289 (1984) Kabat et al., Carbohydr. Res. 130, 289 (1984) Bennett & Bishop, Can. J. Chem. 55, 8 (1977)

6 Cow blood group B gastric mucin derived from stomach Cow26 (blood 3 34 K4 substance, phenol linings of cow no. 26 by phenol group B) insoluble, 10% ppt. extraction and fractional ethanol precipitation (10% ppt). Helix pomatia D-Galactans of the snail From Dr. Niel Glaudemans (NIH, 1 35 K5 galactan H. pomatia Bethesda, MD) Helix nemoralis From Dr. Niel Glaudemans (NIH, 1 36 K6 H. nemoralis galactan galactan Bethesda, MD) IM6-BSA 4 37 M1 Isomaltohexaose-BSA From Dr. D. Zopf (NCI, NIH) IM6-KLH 4 38 M2 Isomaltohexaose-KLH From Dr. D. Zopf (NCI, NIH) KABAT Laboratory (Dr. Kenneth O. Le a (N-1 IO 4 Blood group substance 3 39 M3 NaOH) Lewis a Lloyd), prepared from human cyst fluid by pepsin digestion and ethanol Beiser & Kabat, J. Immunol. 68, (1952) O'Colla, Proc. R. Ir. Acad. Sect. B. Biol. Geol. Chem. Sci. 55B, 165 (1953); Bretting et al., Comp. Biochem. Physiol. 65B, 497 (1980) Correa et al., Carbohydr. Res. 3, 445 (1967) Zopf et al., Methods Enzymol. 50, 163 (1978); Smith et al., Methods Enzymol. 50, 169 (1978) Zopf et al., Methods Enzymol. 50, 163 (1978); Smith et al., Methods Enzymol. 50, 169 (1978) Lloyd et al., Biochemistry 7, 2976 (1968)

7 Human ovarian cyst Cyst 9 (blood 3 40 M4 blood group A group A) substance. Cyst 9. Clinical Dextran Dextran N-150-N 1 41 M5 N150N with average MW of 60 kda Hog blood group Hog blood group O(H) 3 42 M6 substance H substance AGOR 3 43 O1 Agalacto-orosomucoid precipitation. Alkaline elimination was then performed to release Le a oligosaccharides from the protein portion. The cyst fluid of a Nigerian patient was provided by Ada E. Bezer (WHO) a human ovarian cyst fluid provided by Dr. Harold Baer (NIH). Commercial Solvents Co. (Terre Haute, IN) hog gastric mucin powder (Wilson Labs, Chicago, IL) by phenol extraction and fractional ethanol precipitation KABAT Laboratory, prepared by Gilbert Ashwell (NIH). Preparation of orosomucoid was from Dr. Sten Hammarstrom (University of Baer et al., J. Immunol. 82, 183 (1959); Moreno et al., J. Exp. Med. 134, 439 (1971) Beiser & Kabat, J. Immunol. 68, (1952); Allen & Kabat, J. Immunol. 82, , (1959) Kawasaki & Ashwell, J. Biol. Chem. 252, 6536 (1977);

8 Inulin 1 44 O2 Polyfructosan Levan (from Levan (B-512E) 1 45 O3 preparation of B-512-E dextran) Meningococcus group Meningococcus group Y 1 46 O4 Y capsular E. coli K1 capsular E. coli K O5 E. coli K100 capsular E. coli K O6 Stockholm, Sweden) Difco Laboratories (Detroit, MI). A reserve carbohydrate in the roots of the Asteraceae, the largest group of flowering plants. Commercial Solvents Co. (Terre Haute, IN) From the late Prof. Michael Heidelberger (NY University) From Drs. John Robbins and Rachel Schneerson (NICHD, NIH) From Drs. John Robbins and Rachel Schneerson (NICHD, NIH) a Carbohydrate antigens were classified and are indicated in the table as follows: 1, ; 2, glycosaminoglycan; 3, glycoprotein; 4, semisynthetic glycoconjugate.

An Immunochemical Study of the Combining Sites of the Second Lectin Isolated from Bandeiraea simplicifolia (BS II)

University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Virology Papers Virology, Nebraska Center for 1978 An Immunochemical Study of the Combining Sites of the Second Lectin Isolated