LC TASTE AS A NOVEL TOOL FOR THE IDENTIFICATION OF FLAVOUR MODIFYING COMPOUNDS

Size: px
Start display at page:

Download "LC TASTE AS A NOVEL TOOL FOR THE IDENTIFICATION OF FLAVOUR MODIFYING COMPOUNDS"

Transcription

1 LC TASTE AS A NVEL TL FR THE IDENTIFICATIN F FLAVUR MDIFYING CMPUNDS K.V. Reichelt 1, R. Peter 2, M. Roloff 2, J.P. LEY 2, G.E. Krammer 2, and K.-H. Engel Technische Universität München, Chair of General Food Technology, Am Forum 2, D-8535 Freising-Weihenstephan, Germany Symrise GmbH & Co. KG, Flavor & Nutrition, Research & Innovation, P.. Box 1253, D-371 Holzminden, Germany Abstract LC Taste methodology, which allows direct correlation between analytical data and online in-vivo sensory evaluation, was tested for the ability to identify taste modulating compounds from complex plant derived matrices. Tests were carried out using the known flavour modifying compounds homoeriodictyol (HED), sterubin and hesperetin either alone or in combination to evaluate their taste modifying effects after fractionation via high temperature liquid chromatography. In addition, fractions of Herba Santa methanolic extract, containing these three compounds was also tested. Bitter masking and sweet enhancing effects, respectively, were clearly detectable in the relevant fractions. Introduction Flavour and taste modification (bitter masking, sweet or salt enhancing, etc.) is currently an important topic for the food industry (1, 2). In many products the contents of salt and nutritive sweet carbohydrates have to be reduced which leads to a change in taste profile. Compounds such as polyphenols which are added to various types of foods providing health benefits often show bitter taste. These unwanted side effects are in most cases not tolerated by the consumer. Flavour modifiers are commonly defined as substances which have no typical flavouring properties per se but which are able to modify the flavour profile of other flavouring substances. In combination with other flavouring substances and food ingredients these substances are able to modify the flavour profile of flavoured foods. This consequently leads to a modified perception of the foodstuff by panellists and consumers and is reflected in reproducible changes of flavour profiles in sensory (3). In addition to the fact that these compounds have little or no intrinsic taste, but show their effects only in the presence of taste-active compounds, detection of taste modifiers in natural sources is frequently hindered by the complexity of plant extracts which makes the work laborious as well as time and cost intensive. Typical methods developed for the identification flavour modifying compounds are taste dilution analysis (TDA), comparative taste dilution analysis (ctda) or dose over threshold (DoT) analysis, which in general are rather time consuming (4, 5, 6). To accelerate and to simplify the identification of novel taste modifying compounds from plant extracts, an extension of the known LC Taste methodology (7, 8) was developed, which allows correlating data from real-time analysis and in vivo detection of relevant taste modifying compounds by a specially trained sensory panel (Figure 1). 397

2 Figure 1. Schematic representation of LC Taste device. Experimental Chemicals and extracts. Homoeriodictyol (HED) and sterubin were isolated according to (9). Hesperetin was purchased from Sigma (Steinheim, Germany). Dried Eriodictyon californicum leaves (5 g) were treated with 3.2 l of boiling water for one hour to break up the plant material. The plant material was filtered, dried and extracted 3 times with 2. l of methanol each for one hour. The resulting methanolic extract was evaporated to dryness (97.7 g). High temperature high performance liquid chromatography (HTLC). The HPLC apparatus consisted of two pumps (SunChrom HPLC pump SunFlow ; SunChrom, Friedrichsdorf, Germany), an injector ( µl loop; Midas, Spark, AJ Emmen, The Netherlands), a column oven (Polaratherm Series 9; Selerity Technologies Inc., Salt Lake City, Utah, USA), an ELSD detector (Sedex 85 LT- ELSD, Sedere, Alfortville, Cedex, France) and a diode array detector (SunChrom SpectraFlow, SunChrom, Friedrichsdorf, Germany), monitoring the effluent in a wavelength range between nm and nm. Separations were performed with a Hamilton PRP-1 reversed phase column (Hamilton Company, Reno, Nevada, USA) in a 25 x 1 mm semi-preparative scale with a particle size of 1 μm. After injection of the sample ( µl full loop) the analysis was carried out at 1 C isotherm using a water/ethanol gradient with a flow rate of 3 ml/min to elute the compounds. Sensory studies. Thresholds for basic tastes were determined with a panel of specially trained, healthy persons without any reported taste disorders using sucrose, sodium chloride, citric acid, caffeine and mono sodium glutamate either as described in (1) or after fractionation via high temperature liquid chromatography. Masking and enhancing studies were done using paired comparison test method using water fractionated under the same conditions as a reference. Samples were blinded and coded and presented in randomized order. 398

3 Results In order to determine the performance of both the novel LC Taste technology and the panel, taste recognition thresholds for basic tastes were determined. There were no significant differences between LC Taste and conventional determination of thresholds. Furthermore, known taste modifying compounds such as HED, sterubin (9) and hesperetin (11) (Figure 2) were subjected to HTLC, diluted 1: 1 with standard solutions containing caffeine (5 ppm) and sucrose (5 %), respectively, to yield final concentrations of 5 ppm each and evaluated sensorially by the dedicated panel to determine bitter masking and sweet enhancing effects of these compounds (Figure 3). As a comparison, water was fractionated under the same conditions, diluted with the test solutions as described above and presented to the panellists. Normalized modulation probability factor of both samples, showing the presumption of a tested compound or fraction to have taste modulating effects was calculated as follows NMP = ( n n ) higher lower / n total Me Me H Me H Figure 2. Chemical structures of homoeriodictyol (HED), sterubin & hesperetin from Herba Santa (Eriodictyon californicum (H. & A.) Torr., Hydrophyllaceae). normalized modulation probability Hesperetin HED Sterubin Sweet enhancing Bitter masking Figure 3. NMP-factors for hesperetin, HED (tested as sodium salt) and sterubin tested as single compounds (5 ppm each) on caffeine and sucrose solution after fractionation via LC Taste (n= 15). A recombinate from methanolic Herba Santa extract was prepared, containing the before tested compounds HED ( ppm in final solution), hesperetin ( ppm) and sterubin ( ppm) and separated and fractionated via HTLC. The eluate was diluted 1:1 with the testing solutions containing sucrose or caffeine and evaluated by the sensory panel to determine the taste modulating properties of the recombinate fractions in comparison to samples containing no test compound (Figure 4). 399

4 sweet enhancing effects (1), (2) (3) bitter masking effects Figure 4. NMP-factors for hesperetin (1, ppm), HED (2, ppm; tested as sodium salt), and sterubin (3, ppm) tested as recombinate from Herba Santa extract on caffeine and sucrose solution after fractionation via LC Taste (n= 15). In addition, methanolic Herba Santa extract was separated via HTLC and fractionated. Selected fractions, including those containing HED, hesperetin and sterubin were diluted with testing solutions and evaluated sensorially by an expert panel (n= 8) to identify possible taste modulating effects (Figure 5). As described above, samples fractionated under the same conditions but containing no test compound served as reference samples. enhancing effect masking effect bitter sweet no effect Figure 5. NMP-factors for selected peaks from Herba Santa extract tested on sucrose and caffeine solution after fractionation via LC Taste (n= 8). It was shown that the taste modulating effects of the tested single compounds HED, hesperetin and sterubin were detectable by sensory analysis using LC Taste. The same effect was shown for a recombinate of Herba Santa extract, containing the three compounds mentioned above. Herba Santa extract was separated and fractionated via HTLC under the same conditions. Selected peaks were tested for their taste modulating properties using sucrose and caffeine solution as test solution. Two fractions, containing the taste modulating compounds HED, hesperetin and sterubin clearly showed sweet enhancing and bitter masking effects, respectively. In

5 addition to this known active compounds, three other fractions showed interesting sweet enhancing effects. Several other fractions did not seem to reduce but to enhance bitterness. These fractions will be analysed in detail to know which compounds are responsible for these effects. References 1. Ley J.P. (8) Chem. Percept. 1: Ley J.P., Blings M., Paetz S., Kindel G., Freiherr K., Krammer G.E., Bertram H.- J. (8) In Sweetness and Sweeteners Biology, Chemistry, and Psychophysics (Weerasinghe D.K., DuBois G.E., eds.), American Chemical Society: Washington, DC, USA, 8, pp Krammer G., personal communication. 4. ttinger H., Soldo T., Hofmann T. (3) J. Agric. Food Chem. 51: Soldo T., Hofmann T. (5) J. Agric. Food Chem. 53: Scharbert S., Hofmann T. (5) J. Agric. Food Chem. 53: Roloff M.; Erfurt H.; Kindel G.; Schmidt C.-.; Krammer G.E. (6) W 6/ Krammer G. et al. (6) In Flavour Science: Recent Advances and Trends (Bredie W.L.P., Petersen M.A., eds.), Elsevier: Amsterdam, Netherlands, pp Ley J.P.; Krammer G.; Reinders G.; Gatfield I.L.; Bertram H.-J. (5) J. Agric. Food Chem. 53: Deutsches Institut für Normung, e.v. (5) DIN 1959 Sensorische Prüfverfahren - Bestimmung der Geschmacksempfindlichkeit. 11. Ley J.P.; Kindel G.; Paetz S.; Riess T.; Haug M.; Schmidtmann R.; Kammer G.E. (7) W 7/

Mass-Based Purification of Natural Product Impurities Using an Agilent 1260 Infinity II Preparative LC/MSD System

Mass-Based Purification of Natural Product Impurities Using an Agilent 1260 Infinity II Preparative LC/MSD System Application Note Food Testing and Agriculture Mass-Based Purification of Natural Product Impurities Using an Agilent 126 Infinity II Preparative LC/MSD System Authors Florian Rieck and Jörg Hippler Agilent

More information

Rapid Gradient and Elevated Temperature UHPLC of Flavonoids in Citrus Fruit

Rapid Gradient and Elevated Temperature UHPLC of Flavonoids in Citrus Fruit Rapid Gradient and Elevated Temperature UHPLC of Flavonoids in Citrus Fruit Application Note General Chromatography, Food Industry Authors John W. Henderson Jr., Judy Berry, Anne Mack, William Long Agilent

More information

Qualitative and quantitative determination of phenolic antioxidant compounds in red wine and fruit juice with the Agilent 1290 Infinity 2D-LC Solution

Qualitative and quantitative determination of phenolic antioxidant compounds in red wine and fruit juice with the Agilent 1290 Infinity 2D-LC Solution Qualitative and quantitative determination of phenolic antioxidant compounds in red wine and fruit juice with the Agilent 1290 Infinity 2D-LC Solution Application Note Food Testing Author Edgar Naegele

More information

Application Note. Agilent Application Solution Analysis of ascorbic acid, citric acid and benzoic acid in orange juice. Author. Abstract.

Application Note. Agilent Application Solution Analysis of ascorbic acid, citric acid and benzoic acid in orange juice. Author. Abstract. Agilent Application Solution Analysis of ascorbic acid, citric acid and benzoic acid in orange juice Application Note Author Food Syed Salman Lateef Agilent Technologies, Inc. Bangalore, India 8 6 4 2

More information

Analysis of Amino Acids Derived Online Using an Agilent AdvanceBio AAA Column

Analysis of Amino Acids Derived Online Using an Agilent AdvanceBio AAA Column Application Note Pharmaceutical and Food Testing Analysis of Amino Acids Derived Online Using an Agilent AdvanceBio AAA Column Author Lu Yufei Agilent Technologies, Inc. Abstract A liquid chromatographic

More information

Analysis of Common Sweeteners and Additives in Beverages with the PerkinElmer Flexar FX-15 System Equipped with a PDA Detector

Analysis of Common Sweeteners and Additives in Beverages with the PerkinElmer Flexar FX-15 System Equipped with a PDA Detector application Note Liquid Chromatography Author Njies Pedjie PerkinElmer, Inc. Shelton, CT 06484 USA Analysis of Common Sweeteners and Additives in Beverages with the PerkinElmer Flexar FX-15 System Equipped

More information

Analysis of Cannabinoids in Cannabis by UHPLC Using PDA Detection

Analysis of Cannabinoids in Cannabis by UHPLC Using PDA Detection APPLICATION NOTE Liquid Chromatography Author: Wilhad M. Reuter PerkinElmer, Inc. Shelton, CT Analysis of Cannabinoids in Cannabis by UHPLC Using PDA Detection Introduction Cannabis sativa, from which

More information

HPLC Analysis of Sugars

HPLC Analysis of Sugars HPLC Analysis of Sugars Pre-Lab Exercise: 1) Read about HPLC, sugars and the experiment and its background. 2) Prepare a flowchart as appropriate for the lab exercise. 3) Note the various sugar concentrations

More information

Transferring a Method for Analysis of DNPH-Derivatized Aldehydes and Ketones from HPLC to UHPLC

Transferring a Method for Analysis of DNPH-Derivatized Aldehydes and Ketones from HPLC to UHPLC Transferring a Method for Analysis of DNPH-Derivatized Aldehydes and Ketones from HPLC to UHPLC Application Note Environmental Author Melanie Metzlaff Agilent Technologies, Inc. Waldbronn, Germany Abstract

More information

UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes

UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes Catalin E. Doneanu, Weibin Chen, and Jeffrey R. Mazzeo Waters Corporation, Milford, MA, U.S. A P P L I C AT ION B E N E F

More information

Relative Measurement of Zeaxanthin Stereoisomers by Chiral HPLC

Relative Measurement of Zeaxanthin Stereoisomers by Chiral HPLC Relative Measurement of Zeaxanthin Stereoisomers by Chiral HPLC Principle To measure the relative percentages of the (3R,3 R), (3R,3 S) and (3S,3 S) stereoisomers of zeaxanthin in dietary ingredient and

More information

Impurity Profiling of Carbamazepine by HPLC/UV

Impurity Profiling of Carbamazepine by HPLC/UV Application Note: 52049 Impurity Profiling of Carbamazepine by HPLC/UV Terry Zhang, Guifeng Jiang, Thermo Fisher Scientific, San Jose, CA, USA Key Words Accela Hypersil GOLD Carbamazepine Drug Analysis

More information

Product Guide for LudgerSep TM R1 HPLC Column for DMB labelled Sialic Acid Analysis

Product Guide for LudgerSep TM R1 HPLC Column for DMB labelled Sialic Acid Analysis Product Guide for LudgerSep TM R1 HPLC Column for DMB labelled Sialic Acid Analysis Product # LS-R1-4.6x150 Ludger Document # LS-R1-DMB-Guide-v5.1 Ludger Ltd Culham Science Centre Oxford OX14 3EB United

More information

Extended Temperature Range Impact on HPLC Column Stability and Performance

Extended Temperature Range Impact on HPLC Column Stability and Performance Extended Temperature Range Impact on HPLC Column Stability and Performance Stephanie J. Marin Dale Felix, Jody Clark Selerity Technologies, Inc. Salt Lake City, UT www.selerity.com The Selerity Series

More information

ISSN: ; CODEN ECJHAO E-Journal of Chemistry 2011, 8(3),

ISSN: ; CODEN ECJHAO E-Journal of Chemistry  2011, 8(3), ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2011, 8(3), 1275-1279 Simultaneous Determination of Paracetamol, Phenylephrine Hydrochloride, Oxolamine Citrate and Chlorpheniramine

More information

Application Note. Authors: C. Ledesma, M. Gibert, J.R. Gibert Ingenieria Analitica S.L. Extracts from various food products

Application Note. Authors: C. Ledesma, M. Gibert, J.R. Gibert Ingenieria Analitica S.L. Extracts from various food products High-Performance Anion-Exchange Chromatography coupled with Pulsed Electrochemical Detection as a powerful tool to evaluate lactose content in lactose-free labeled products Application Note Authors: C.

More information

Comparison of a UPLC Method across Multiple UHPLC Systems

Comparison of a UPLC Method across Multiple UHPLC Systems Comparison of a UPLC Method across Multiple UHPLC Systems Tanya Jenkins Waters Corporation, Milford, MA, U.S. INTRODUCTION In 2004, Waters introduced the ACQUITY UPLC System. Since this launch, many liquid

More information

Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 82 nd meeting 2016.

Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 82 nd meeting 2016. Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 82 nd meeting 2016 Quinoline Yellow This monograph was also published in: Compendium of Food Additive

More information

Chiral Multicolumn Method Development on the Agilent 1260 Infinity II SFC System

Chiral Multicolumn Method Development on the Agilent 1260 Infinity II SFC System Chiral Multicolumn Method Development on the Agilent 126 Infinity II SFC System Application Note Small Molecule Pharmaceuticals Author Edgar Naegele Agilent Technologies, Inc. Waldbronn, Germany Abstract

More information

Amudha S et al., Asian Journal of Pharmthiaceutical Technology & Innovation, 04 (21); 2016; Research Article

Amudha S et al., Asian Journal of Pharmthiaceutical Technology & Innovation, 04 (21); 2016; Research Article Asian Journal of Pharmaceutical Technology & Innovation ISSN: 2347-8810 Research Article Received on: 09-11-2016 Accepted on: 20-11-2016 Published on: 15-12-2016 Corresponding Author: * Amudha S, Dept.

More information

Simultaneous Analysis of Active Pharmaceutical Ingredients and Their Counter-Ions Using a Mixed-Mode Column

Simultaneous Analysis of Active Pharmaceutical Ingredients and Their Counter-Ions Using a Mixed-Mode Column P-CN54E Simultaneous Analysis of Active Pharmaceutical Ingredients and Their Counter-Ions Using a Mixed-Mode Column Pittcon 5 9-6P Kenichiro Tanaka, William Hedgepeth, Yuki Sato Shimadzu Scientific Instruments,

More information

Rapid and sensitive UHPLC screening of additives in carbonated beverages with a robust organic acid column

Rapid and sensitive UHPLC screening of additives in carbonated beverages with a robust organic acid column APPLICATION NOTE 21673 Rapid and sensitive UHPLC screening of additives in carbonated beverages with a robust organic acid column Authors Aaron Lamb and Brian King, Thermo Fisher Scientific, Runcorn, UK

More information

ASSAY AND IMPURITY METHOD FOR DURACOR TABLETS BY HPLC

ASSAY AND IMPURITY METHOD FOR DURACOR TABLETS BY HPLC ASSAY AND IMPURITY METHOD FOR DURACOR TABLETS BY HPLC METHOD APPROVALS Norvin Pharma Inc. Author Analytical Laboratory Approver Analytical Laboratory Group Leader Approver Manager Quality Control Chemistry

More information

[ APPLICATION NOTE ] Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector

[ APPLICATION NOTE ] Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector Mark Benvenuti, Gareth Cleland, and Jennifer Burgess Waters Corporation, Milford, MA, USA

More information

Ankit et al Journal of Drug Delivery & Therapeutics; 2013, 3(2), Available online at RESEARCH ARTICLE

Ankit et al Journal of Drug Delivery & Therapeutics; 2013, 3(2), Available online at  RESEARCH ARTICLE Ankit et al Journal of Drug Delivery & Therapeutics; 2013, 3(2), 26-30 26 Available online at http://jddtonline.info RESEARCH ARTICLE METHOD DEVELOPMENT AND ITS VALIDATION FOR QUANTITATIVE SIMULTANEOUS

More information

Analysis of Commercially Available Products Containing Stevia

Analysis of Commercially Available Products Containing Stevia Analysis of Commercially Available Products Containing Stevia Application note The commercial use of the herb stevia refers to the extracts from the Stevia rebaudina, Bertoni plant. Although originally

More information

Analysis of Phenolic Antioxidants in Edible Oil/Shortening Using the PerkinElmer Altus UPLC System with PDA Detection

Analysis of Phenolic Antioxidants in Edible Oil/Shortening Using the PerkinElmer Altus UPLC System with PDA Detection APPLICATION NOTE Liquid Chromatography Author: Wilhad M. Reuter PerkinElmer, Inc. Shelton, CT Analysis of Phenolic Antioxidants in Edible Oil/Shortening Using the PerkinElmer Altus UPLC System with PDA

More information

Maximizing chromatographic peak capacity with the Agilent 1290 Infinity LC system

Maximizing chromatographic peak capacity with the Agilent 1290 Infinity LC system Maximizing chromatographic peak capacity with the Agilent 1290 Infinity LC system A practical guide on how to use parameters to increase peak capacity Application Note Pharmaceutical and Chemical Author

More information

Product Guide for LudgerSep TM ur2 UHPLC Column for DMB Sialic Acid Analysis

Product Guide for LudgerSep TM ur2 UHPLC Column for DMB Sialic Acid Analysis Product Guide for LudgerSep TM ur2 UHPLC Column for DMB Sialic Acid Analysis Product # LS-UR2-2.1x100 Ludger Document # LS-uR2-DMB-Guide-v2.1 Ludger Ltd Culham Science Centre Oxford OX14 3EB United Kingdom

More information

STANDARD OPERATING PROTOCOL (SOP)

STANDARD OPERATING PROTOCOL (SOP) 1 STANDARD PERATING PRTCL (SP) Subject: To determine the Contents of isoflavone in soybean products by HPLC Analysis Project/Core No.: Core B Total 9 Pages SP No.: CB0102 Modified Date: 05/15/01 BTANICAL

More information

PAPRIKA EXTRACT SYNONYMS DEFINITION DESCRIPTION FUNCTIONAL USES CHARACTERISTICS

PAPRIKA EXTRACT SYNONYMS DEFINITION DESCRIPTION FUNCTIONAL USES CHARACTERISTICS PAPRIKA EXTRACT Prepared at the 77 th JECFA, published in FAO JECFA Monographs 14 (2013), superseding tentative specifications prepared at the 69 th JECFA (2008). An ADI of 0-1.5 mg/kg bw was allocated

More information

Application Note. Introduction

Application Note. Introduction by Supercritical Fluid Extraction and Supercritical Fluid Chromatography with Evaporative Light Scattering Detection Introduction An evaporative light scattering detector (ELSD) is a versatile detector

More information

SUPERCRITICAL FLUID EXTRACTION OF MICROALGAE SPIRULINA PLATENSIS. CHEMO-FUNCTIONAL CHARACTERIZATION

SUPERCRITICAL FLUID EXTRACTION OF MICROALGAE SPIRULINA PLATENSIS. CHEMO-FUNCTIONAL CHARACTERIZATION SUPERCRITICAL FLUID EXTRACTION OF MICROALGAE SPIRULINA PLATENSIS. CHEMO-FUNCTIONAL CHARACTERIZATION Jose A. Mendiola 1, F. Javier Señoráns 1, Elena Ibañez* 2, Laura Jaime 1 and Guillermo Reglero 1 1: Área

More information

Determination of Tetracyclines in Chicken by Solid-Phase Extraction and High-Performance Liquid Chromatography

Determination of Tetracyclines in Chicken by Solid-Phase Extraction and High-Performance Liquid Chromatography Determination of Tetracyclines in Chicken by Solid-Phase Extraction and High-Performance Liquid Chromatography Application ote Food Safety Authors Chen-Hao Zhai and Yun Zou Agilent Technologies Co. Ltd.

More information

STANDARD OPERATING PROTOCOL (SOP)

STANDARD OPERATING PROTOCOL (SOP) 1 STANDARD PERATING PRTCL (SP) Subject: Determination of Flavonol Glycosides in Ginkgo biloba Products by HPLC Analysis Project/Core No.: Core B Total 6 Pages SP No.: CB0104 Modified Date: 07/30/01 BTANICAL

More information

Hard resins: New insights into a long-known hop fraction. Martin Biendl

Hard resins: New insights into a long-known hop fraction. Martin Biendl Hard resins: New insights into a long-known hop fraction Martin Biendl Woellmer hop fractionation (invented 1916) soluble in methanol: total resins soluble in hexane: soft resins approx. 90 % (w/w) alpha-acids

More information

Application Note. Agilent Application Solution Analysis of fat-soluble vitamins from food matrix for nutrition labeling. Abstract.

Application Note. Agilent Application Solution Analysis of fat-soluble vitamins from food matrix for nutrition labeling. Abstract. Agilent Application Solution Analysis of fat-soluble vitamins from food matrix for nutrition labeling Application Note Food 1 Agilent 12 Infinity 1 Author Siji Joseph Agilent Technologies, Inc. Bangalore,

More information

Agilent 1260 Infinity Analytical SFC System

Agilent 1260 Infinity Analytical SFC System Agilent 1260 Infinity Analytical SFC System Installation Instructions: Checkout Procedure This technical note describes the checkout procedures for the Agilent 1260 Infinity Analytical SFC System. Contents

More information

A Robustness Study for the Agilent 6470 LC-MS/MS Mass Spectrometer

A Robustness Study for the Agilent 6470 LC-MS/MS Mass Spectrometer A Robustness Study for the Agilent 7 LC-MS/MS Mass Spectrometer Application Note Clinical Research Authors Linda Côté, Siji Joseph, Sreelakshmy Menon, and Kevin McCann Agilent Technologies, Inc. Abstract

More information

LC/MS Analysis of Various Hydrophilic Compounds Using a Polymer-Based Amino Column - Shodex TM HILICpak TM VG-50 2D

LC/MS Analysis of Various Hydrophilic Compounds Using a Polymer-Based Amino Column - Shodex TM HILICpak TM VG-50 2D LC/MS Analysis of Various Hydrophilic Compounds Using a Polymer-Based Amino Column - Shodex TM HILICpak TM VG-50 2D Introduction Components of pharmaceutical products and food products often include high

More information

APPLICATION NOTE Determination of Aflatoxin M1 via FREESTYLE ThermELUTE with Online HPLC-Measurement

APPLICATION NOTE Determination of Aflatoxin M1 via FREESTYLE ThermELUTE with Online HPLC-Measurement Determination of Aflatoxin M1 via FREESTYLE ThermELUTE with Online HPLC-Measurement APPLICATION NOTE www.lctech.de As of: February 2017, Version: 1.3 Determination of Aflatoxin M1 via FREESTYLE ThermELUTE

More information

Journal of Chemical and Pharmaceutical Research

Journal of Chemical and Pharmaceutical Research Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(2):770-775 Validation of Rapid Liquid Chromatographic

More information

Separation of Polyphenols by Comprehensive 2D-LC and Molecular Formula Determination by Coupling to Accurate Mass Measurement

Separation of Polyphenols by Comprehensive 2D-LC and Molecular Formula Determination by Coupling to Accurate Mass Measurement Separation of Polyphenols by Comprehensive 2D-LC and Molecular Formula Determination by Coupling to Accurate Mass Measurement Application Note Food Testing & Agriculture Author Edgar Naegele Agilent Technologies,

More information

Quantitative Analysis of Carbohydrates and Artificial Sweeteners in Food Samples Using GFC- MS with APCI Interface and Post-column Reagent Addition

Quantitative Analysis of Carbohydrates and Artificial Sweeteners in Food Samples Using GFC- MS with APCI Interface and Post-column Reagent Addition PO-CON1321E Quantitative Analysis of Carbohydrates and Artificial Sweeteners in Food Samples Using GFC- MS with APCI Interface and Post-column Reagent Addition ASMS 213 TP 71 Jie Xing 1, Yin Ling Chew*

More information

The Determination of Sugars in Molasses by High-Performance Anion Exchange with Pulsed Amperometric Detection

The Determination of Sugars in Molasses by High-Performance Anion Exchange with Pulsed Amperometric Detection Application Note 9 The Determination of Sugars in Molasses by High-Performance Anion Exchange with Pulsed Amperometric Detection INTRODUCTION The accurate measurement of the amount of sugar in final molasses

More information

Determination of Benzoate in Liquid Food Products by Reagent-Free Ion Chromatography

Determination of Benzoate in Liquid Food Products by Reagent-Free Ion Chromatography Application Note 65 Determination of Benzoate in Liquid Food Products by Reagent-Free Ion Chromatography INTRODUCTION Preservatives are commonly added to many food products, such as soda, fruit juice,

More information

Determination of sugars in honey by liquid chromatography

Determination of sugars in honey by liquid chromatography Saudi Journal of Biological Sciences (2011) 18, 17 21 King Saud University Saudi Journal of Biological Sciences www.ksu.edu.sa www.sciencedirect.com ORIGINAL ARTICLE Determination of sugars in honey by

More information

SUPPLEMENTARY MATERIAL

SUPPLEMENTARY MATERIAL SUPPLEMENTARY MATERIAL Artepillin C, is it a good marker for quality control of Brazilian Green Propolis? Cui-ping Zhang 1, Xiao-ge Shen 1, Jia-wei Chen 1, Xia-sen Jiang 1, Kai Wang 2, Fu-liang Hu 1 *

More information

Application Note. Authors. Abstract. Petrochemical

Application Note. Authors. Abstract. Petrochemical Fast screening of impurities in biodiesel using the Agilent 160 Infinity Analytical SFC System in combination with evaporative light scattering detection Application Note Petrochemical Authors Maria Rambla-Alegre,

More information

SEDEX 90LT Presentation

SEDEX 90LT Presentation SEDEX 90LT Presentation HPLC 2011, Budapest SENSITIVITY FLEXIBILITY SEDERE EXPERIENCE SEDEX 90LT Technology SEDEX 990LT 0LT Light Source Selected Blue-Violet Laser 405nm 10mW Output SEDEX 90LT Technology

More information

SIMPLE AND VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF CARBOPLATIN IN BULK AND FORMULATION DOSAGE FORM. Subhashini.Edla* B.

SIMPLE AND VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF CARBOPLATIN IN BULK AND FORMULATION DOSAGE FORM. Subhashini.Edla* B. SIMPLE AND VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF CARBOPLATIN IN BULK AND FORMULATION DOSAGE FORM Subhashini.Edla* B.Syama sundhar Abstract Dept of Chemistry, Acharya Nagarjuna University, Nagarjuna

More information

Optimization of extraction method and profiling of plant phenolic compounds through RP-HPLC

Optimization of extraction method and profiling of plant phenolic compounds through RP-HPLC Chapter III Optimization of extraction method and profiling of plant phenolic compounds through RP-HPLC 1. INTRODUCTION Phenolics compounds are naturally present antioxidants, found in a variety of plant

More information

Automated Purification and Analytical Reinjection of a Small Molecule Drug, Probenecid, on a Gilson LC/MS Dual Function System

Automated Purification and Analytical Reinjection of a Small Molecule Drug, Probenecid, on a Gilson LC/MS Dual Function System Automated Purification and Analytical Reinjection of a Small Molecule Drug, Probenecid, on a Gilson LC/MS Dual Function System Keywords Introduction Application Note PHA0413 High Pressure Liquid Chromatography

More information

Fig.1. Denatonium benzoate (DB) chemical structure

Fig.1. Denatonium benzoate (DB) chemical structure ILIADE code 280 CLEN Method Determination of Denatonium Benzoate in Alcoholic Products by HPLC-UV 1 Scope The purpose of this method is verification of fulfilment of the legislative requirements on denatured

More information

Determination of 6-Chloropicolinic Acid (6-CPA) in Crops by Liquid Chromatography with Tandem Mass Spectrometry Detection. EPL-BAS Method No.

Determination of 6-Chloropicolinic Acid (6-CPA) in Crops by Liquid Chromatography with Tandem Mass Spectrometry Detection. EPL-BAS Method No. Page 1 of 10 Determination of 6-Chloropicolinic Acid (6-CPA) in Crops by Liquid Chromatography with Tandem Mass Spectrometry Detection EPL-BAS Method No. 205G881B Method Summary: Residues of 6-CPA are

More information

Analysis of Cannabinoids in Hemp Seed Oils by HPLC Using PDA Detection

Analysis of Cannabinoids in Hemp Seed Oils by HPLC Using PDA Detection APPLICATION NOTE Liquid Chromatography Authors: Catharine Layton Wilhad M. Reuter PerkinElmer, Inc. Shelton, CT Analysis of Cannabinoids in Hemp Seed Oils by HPLC Using PDA Detection Introduction Cannabis

More information

ANALYSIS OF -HYDROXYBUTYRATE (GHB) AND -BUTYROLACTONE (GBL) IN LIQUIDS PERFORMED AT NATIONAL LABORATORY OF FORENSIC SCIENCE (SKL), SWEDEN

ANALYSIS OF -HYDROXYBUTYRATE (GHB) AND -BUTYROLACTONE (GBL) IN LIQUIDS PERFORMED AT NATIONAL LABORATORY OF FORENSIC SCIENCE (SKL), SWEDEN ANALYSIS OF -HYDROXYBUTYRATE (GHB) AND -BUTYROLACTONE (GBL) IN LIQUIDS PERFORMED AT NATIONAL LABORATORY OF FORENSIC SCIENCE (SKL), SWEDEN Per LUNDQUIST National Laboratory of Forensic Science, Linköping,

More information

Improved Extraction and Analysis of Hexavalent Chromium from Soil and Water

Improved Extraction and Analysis of Hexavalent Chromium from Soil and Water Improved Extraction and Analysis of Hexavalent Chromium from Soil and Water Richard F. Jack, 1 Jinshui Che, 2 Lipika Basumallick, 1 and Jeffrey Rohrer 1 1 Thermo Fisher Scientific, Sunnyvale, CA, USA;

More information

CHAPTER 8 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS OF M. ROXBURGHIANUS AND P. FRATERNUS PLANT EXTRACTS

CHAPTER 8 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS OF M. ROXBURGHIANUS AND P. FRATERNUS PLANT EXTRACTS CHAPTER 8 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS OF M. ROXBURGHIANUS AND P. FRATERNUS PLANT EXTRACTS CHAPTER 8: HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

More information

Application Note Soy for Isoflavones by HPLC. Botanical Name: Glycine max L. Common Names: Parts of Plant Used: Beans.

Application Note Soy for Isoflavones by HPLC. Botanical Name: Glycine max L. Common Names: Parts of Plant Used: Beans. Application Note 0066 - Soy for Isoflavones by HPLC As published in The Handbook of Analytical Methods for Dietary Supplements Botanical Name: Glycine max L. Common Names: Soybean Parts of Plant Used:

More information

Performance Characteristics of the Agilent 1290 Infinity II Multicolumn Thermostat

Performance Characteristics of the Agilent 1290 Infinity II Multicolumn Thermostat Performance Characteristics of the Agilent 9 Infinity II Multicolumn Thermostat Technical Overview Author Sonja Schneider Agilent Technologies, Inc. Waldbronn, Germany Abstract This Technical Overview

More information

Title Revision n date

Title Revision n date A. THIN LAYER CHROMATOGRAPHIC TECHNIQUE (TLC) 1. SCOPE The method describes the identification of hydrocortisone acetate, dexamethasone, betamethasone, betamethasone 17-valerate and triamcinolone acetonide

More information

Effective use of Pharmacopeia guidelines to reduce cost of chromatographic analysis for Fluticasone propionate

Effective use of Pharmacopeia guidelines to reduce cost of chromatographic analysis for Fluticasone propionate Effective use of Pharmacopeia guidelines to reduce cost of chromatographic analysis for Fluticasone propionate Application Note Pharmaceutical QA/QC Author Siji Joseph Agilent Technologies, Inc. Bangalore,

More information

Analysis of Cannabinoids in Hemp Seed Oils by HPLC Using PDA Detection

Analysis of Cannabinoids in Hemp Seed Oils by HPLC Using PDA Detection A P P L I C AT I O N N O T E Liquid Chromatography Authors: Catharine Layton Wilhad M. Reuter PerkinElmer, Inc. Shelton, CT Analysis of Cannabinoids in Hemp Seed Oils by HPLC Using PDA Detection Introduction

More information

Type Analysis of Citrus Essential Oils by Multidimensional Supercritical Fluid Chromatography/ Gas Chromatography

Type Analysis of Citrus Essential Oils by Multidimensional Supercritical Fluid Chromatography/ Gas Chromatography 25 Type Analysis of Citrus Essential Oils by Multidimensional Supercritical Fluid Chromatography/ Gas Chromatography Takashi YARITA, Akira NoMURA and Yoshiyuki HoRIMOTo Department of Analytical Chemistry,

More information

Application Note. Authors. Abstract. Food

Application Note. Authors. Abstract. Food Determination of Hormones in Shrimp by Agilent 129 Infinity LC with Agilent Poroshell 12 LC Column and Agilent Bond Elut QuEChERS for Sample Preparation Application Note Food Authors Rongjie Fu and Andy

More information

Analysis of Counterfeit Antidiabetic Drugs by UHPLC with the Agilent 1220 Infinity Mobile LC

Analysis of Counterfeit Antidiabetic Drugs by UHPLC with the Agilent 1220 Infinity Mobile LC Analysis of Counterfeit Antidiabetic Drugs by UPLC with the Agilent 1 Infinity Mobile LC Application Note Small Molecule Pharmaceuticals & Generics Author Sonja Schneider Agilent Technologies, Inc. Waldbronn,

More information

Application Note. Small Molecule Pharmaceuticals. 2-Ethyl-2-phenylmalonamide Ethosuximide Primidone Carbamazepine. Carbamazepine-

Application Note. Small Molecule Pharmaceuticals. 2-Ethyl-2-phenylmalonamide Ethosuximide Primidone Carbamazepine. Carbamazepine- Analysis of Pharmaceutical Substances Using HPLC and UHPLC Methods Backward Compatibility for Validated Analytical Procedures and Methods Comparing the Infi nity LC and the Infi nity II LC Application

More information

Minimierung von Kochsalz bei Brot und Backwaren: Technologie und Geschmackqualität IGF-Projekt Nr N

Minimierung von Kochsalz bei Brot und Backwaren: Technologie und Geschmackqualität IGF-Projekt Nr N .7. Minimierung von Kochsalz bei Brot und Backwaren: Technologie und Geschmackqualität IGF-Projekt Nr. 5 N Peter Köhler, Katharina Konitzer, Tabea Wieber, Thomas Hofmann Deutsche Forschungsanstalt für

More information

AS Application Note 1801

AS Application Note 1801 Determination of mineral oil contaminations in foodstuff, cosmetics and packaging with the CHRONECT Workstation MOSH/MOAH ASAN 1801 Status: April 2018 Page 1 / 8 Introduction Mineral oil contaminants are

More information

Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 1290 Infinity II/6230B LC/TOF system

Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 1290 Infinity II/6230B LC/TOF system Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 9 Infinity II/63B LC/TOF system Application Brief Authors Mike Adams, Karen Kaikaris, and A. Roth CWC Labs Joan Stevens, Sue D

More information

Pelagia Research Library

Pelagia Research Library Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2014, 5(5):91-98 ISSN: 0976-8688 CODEN (USA): PSHIBD A novel RP-HPLC method development and validation of Perindopril Erbumine in

More information

ISSN X CODEN (USA): PCHHAX. A study of the component composition of phenolic compounds obtained from Dahlia varieties Ken s Flame herb

ISSN X CODEN (USA): PCHHAX. A study of the component composition of phenolic compounds obtained from Dahlia varieties Ken s Flame herb Available online at www.derpharmachemica.com ISSN 0975-413X CODEN (USA): PCHHAX Der Pharma Chemica, 2016, 8(18):455-459 (http://derpharmachemica.com/archive.html) A study of the component composition of

More information

Food Matters Live Sensory marketing in practice Applying sensory branding in a health and well being context

Food Matters Live Sensory marketing in practice Applying sensory branding in a health and well being context Food Matters Live Sensory marketing in practice Applying sensory branding in a health and well being context Peter Burgess Campden BRI, UK 24 th November 2016 Topic areas Product development challenges

More information

Rapid and Robust Detection of THC and Its Metabolites in Blood

Rapid and Robust Detection of THC and Its Metabolites in Blood Rapid and Robust Detection of THC and Its Metabolites in Blood Application Note Forensics/Doping Control Author Stephan Baumann Agilent Technologies, Inc. Santa Clara CA 95051 USA Abstract A robust method

More information

A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY FOR LERCANIDIPINE HYDROCHLORIDE

A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY FOR LERCANIDIPINE HYDROCHLORIDE Int. J. Chem. Sci.: 6(1), 2008, 441-446 A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY FOR LERCANIDIPINE HYDROCHLORIDE S. APPALA RAJU, ARVIND B. KARADI and SHOBHA MANJUNATH HKES s College of Pharmacy,

More information

Official Journal of the European Union. (Non-legislative acts) REGULATIONS

Official Journal of the European Union. (Non-legislative acts) REGULATIONS 23.4.2016 L 108/1 II (Non-legislative acts REGULATIONS COMMISSION IMPLEMTING REGULATION (EU 2016/635 of 22 April 2016 amending the Annex to Regulation (EC No 2870/2000 aegards certain reference methods

More information

10 Sulfaquinoxaline H N O S O. 4-amino-N-quinoxalin-2-ylbenzenesulfonamide C 14 H 12 N 4 O 2 S MW: CAS No.:

10 Sulfaquinoxaline H N O S O. 4-amino-N-quinoxalin-2-ylbenzenesulfonamide C 14 H 12 N 4 O 2 S MW: CAS No.: 10 Sulfaquinoxaline N N H N O S O NH 2 4-amino-N-quinoxalin-2-ylbenzenesulfonamide C 14 H 12 N 4 O 2 S MW: 300.33 CAS No.: 59-40-5 Outline of sulfaquinoxaline Sulfaquinoxaline is light yellow to brownish

More information

Product Guide for LudgerSep TM R1 HPLC Column for Glycan Analysis

Product Guide for LudgerSep TM R1 HPLC Column for Glycan Analysis Product Guide for LudgerSep TM R1 HPLC Column for Glycan Analysis (Ludger Product Code: LS-R1-4.6x150) Ludger Ltd Culham Science Centre Oxford OX14 3EB United Kingdom Tel: +44 1865 408 554 Fax: +44 870

More information

1 out of 8. Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 86th Meeting 2018 ERYTHROSINE

1 out of 8. Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 86th Meeting 2018 ERYTHROSINE 1 out of 8 Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 86th Meeting 2018 ERYTHROSINE This monograph was also published in: Compendium of Food

More information

Column Stability and Performance under High Temperature HPLC Conditions

Column Stability and Performance under High Temperature HPLC Conditions Column Stability and Performance under High Temperature HPLC Conditions Stephanie J. Marin Brian Jones, Dale Felix, Jody Clark Selerity Technologies, Inc. Salt Lake City, UT www.selerity.com Introduction

More information

Increasing resolution using longer columns while maintaining analysis time Advantages of the wide power range of the Agilent 1290 Infinity LC System

Increasing resolution using longer columns while maintaining analysis time Advantages of the wide power range of the Agilent 1290 Infinity LC System Increasing resolution using longer columns while maintaining analysis time Advantages of the wide power range of the Agilent 129 Infinity LC System Application Note Pharmaceutical and Chemical Analysis

More information

Journal of Chemical and Pharmaceutical Research

Journal of Chemical and Pharmaceutical Research Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(1):138-144 Simultaneous RP HPLC determination of Latanoprost

More information

Analytical and Preparative SFC Columns

Analytical and Preparative SFC Columns Analytical and Preparative SFC Columns Best Performance for Supercritical Fluid Chromatography Sepax SFC-Cyano... Sepax SFC-Amino... Sepax SFC-Pyridine... Sepax SFC-SCX... 5 Sepax SFC-Diol... 6 Sepax SFC-Silica...

More information

Ultrafast analysis of synthetic antioxidants in vegetable oils using the Agilent 1290 Infinity LC system

Ultrafast analysis of synthetic antioxidants in vegetable oils using the Agilent 1290 Infinity LC system Ultrafast analysis of synthetic antioxidants in vegetable oils using the Agilent 19 Infinity LC system Application Note Food Authors Standard solution Gerd Vanhoenacker, Frank David, Pat Sandra Research

More information

CHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW

CHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW 132 CHAPTER 6 DEVELOPMENT AND VALIDATION OF A STABILITY-INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF PARACETAMOL, TRAMADOL HYDROCHLORIDE AND DOMPERIDONE IN A COMBINED DOSAGE FORM 6.1 INTRODUCTION

More information

Analysis of Food Sugars in Various Matrices Using UPLC with Refractive Index (RI) Detection

Analysis of Food Sugars in Various Matrices Using UPLC with Refractive Index (RI) Detection Analysis of Food Sugars in Various Matrices Using UPLC with Refractive Index (RI) Detection Mark E. Benvenuti Waters Corporation, Milford, MA, USA APPLICATION BENEFITS Using UPLC with RI detection enables

More information

HPLC to UHPLC Transfer of USP Method for Amlodipine Besylate Using the Agilent 1290 Infinity II LC

HPLC to UHPLC Transfer of USP Method for Amlodipine Besylate Using the Agilent 1290 Infinity II LC HPLC to UHPLC Transfer of USP Method for Amlodipine Besylate Using the Agilent 129 Infinity II LC Application Note Small Molecule Pharmaceuticals Authors Gerd Vanhoenacker, Mieke Steenbeke, Koen Sandra,

More information

Bioequivalence Studies of Two Formulations of Famciclovir Tablets by HPLC Method

Bioequivalence Studies of Two Formulations of Famciclovir Tablets by HPLC Method Asian Journal of Chemistry Vol. 19, No. 6 (2007), 4245-4250 Bioequivalence Studies of Two Formulations of Famciclovir Tablets by HPLC Method K.V. SUBRAHMANYAM*, P. MOHANRAJ, P. SANDHYARANI, V.S. SARAVANAN

More information

Supporting Information

Supporting Information Notes Bull. Korean Chem. Soc. 2013, Vol. 34, No. 1 1 http://dx.doi.org/10.5012/bkcs.2013.34.1.xxx Supporting Information Chemical Constituents of Ficus drupacea Leaves and their α-glucosidase Inhibitory

More information

SUMMARY, CONCLUSION & RECOMMENDATIONS

SUMMARY, CONCLUSION & RECOMMENDATIONS 196 Chapter-5 SUMMARY, CONCLUSION & RECOMMENDATIONS 197 CHAPTER 5 5.1 Summary, Conclusion and Recommendations Summary and Conclusion are drawn based on the work carried out by the author on development

More information

Determination of Benzoic Acid in Cranberry (Vaccinium Macrocarpon Ait) by Hplc with Using Different Extraction Methods

Determination of Benzoic Acid in Cranberry (Vaccinium Macrocarpon Ait) by Hplc with Using Different Extraction Methods "Science Stays True Here" Biological and Chemical Research (ISSN 2312-0088), Vol.6, 8-13 Science Signpost Publishing Determination of Benzoic Acid in Cranberry (Vaccinium Macrocarpon Ait) by Hplc with

More information

Annex to the Accreditation Certificate D-PL according to DIN EN ISO/IEC 17025:2005

Annex to the Accreditation Certificate D-PL according to DIN EN ISO/IEC 17025:2005 Deutsche Akkreditierungsstelle GmbH Annex to the Accreditation Certificate D-PL-14285-01-00 according to DIN EN ISO/IEC 17025: Period of validity: 03.02.2017 to 14.09.2021 Holder of certificate: ADM WILD

More information

Draft monograph for inclusion in. The International Pharmacopoeia. Dextromethorphani solutionum peroralum - Dextromethorphan oral solution

Draft monograph for inclusion in. The International Pharmacopoeia. Dextromethorphani solutionum peroralum - Dextromethorphan oral solution August 2015 Draft document for comment 1 2 3 4 5 6 Draft monograph for inclusion in The International Pharmacopoeia Dextromethorphani solutionum peroralum - Dextromethorphan oral solution (August 2015)

More information

Doubling the throughput of long chromatographic methods by using a novel Dual LC workflow

Doubling the throughput of long chromatographic methods by using a novel Dual LC workflow APPLICATIN NTE 7601 Doubling the throughput of long chromatographic methods by using a novel Dual LC workflow Authors Sylvia Grosse, Mauro De Pra, Frank Steiner Thermo Fisher Scientific, Germering, Germany

More information

METHOD 8316 ACRYLAMIDE, ACRYLONITRILE AND ACROLEIN BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

METHOD 8316 ACRYLAMIDE, ACRYLONITRILE AND ACROLEIN BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) METHOD 8316 ACRYLAMIDE, ACRYLONITRILE AND ACROLEIN BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) 1.0 SCOPE AND APPLICATION 1.1 The following compounds can be determined by this method: Compound Name

More information

2D-LC as an Automated Desalting Tool for MSD Analysis

2D-LC as an Automated Desalting Tool for MSD Analysis 2D-LC as an Automated Desalting Tool for MSD Analysis Direct Mass Selective Detection of a Pharmaceutical Peptide from an MS-Incompatible USP Method Application Note Biologics and Biosimilars Author Sonja

More information

Extended Application Note

Extended Application Note Extended Application Note Comparison of 4 μm vs. 2.o Columns in Analysis of Drospirenone/Ethinyl Estradiol Tablet APP A-339 www.mtc-usa.com 1-732-578-1777 INTRODUCTION The formulation of drospirenone and

More information

Delineation of the Role of Glycosylation in the Cytotoxic Properties of Quercetin using Novel Assays in Living Vertebrates

Delineation of the Role of Glycosylation in the Cytotoxic Properties of Quercetin using Novel Assays in Living Vertebrates Delineation of the Role of Glycosylation in the Cytotoxic Properties of Quercetin using Novel Assays in Living Vertebrates Si-Hwan Park,, Hyun Jung Kim,, Soon-Ho Yim, Ah-Ra Kim, Nisha Tyagi, Haihong Shen,

More information

INDUSTRIAL TRAINING REPORT

INDUSTRIAL TRAINING REPORT INDUSTRIAL TRAINING REPORT NORANIZA BINTI AHMAD DAUD BACHELOR OF SCIENCE (HONS) MAJOR IN CHEMISTRY FACULTY OF SCIENCE UNIVERSITY OF PUTRA MALAYSIA CENTER FOR INTERNATIONAL EDUCATION AND RESEARCH FOOD CHEMISTRY,

More information