Chem Lecture 8 Carbohydrate Metabolism Part I: Glycolysis

Transcription

1 Chem Lecture 8 Carbohydrate Metabolism Part I: Glycolysis

2 Introduction Carbohydrate metabolism involves a collection of pathways. Glycolysis Hexoses 3-Carbon molecules Gluconeogenesis 3-Carbon molecules Hexoses Fermentation (anaerobic) Citric Acid Cycle (aerobic) Oxidation all the way to CO 2 + H 2 O Pentose-Phosphate pathway Hexose Pentose 2

3 Introduction Carbohydrate metabolism involves a collection of pathways. Glycolysis Hexoses 3-Carbon molecules Gluconeogenesis 3-Carbon molecules Hexoses Fermentation (anaerobic) Citric Acid Cycle (aerobic) Oxidation all the way to CO 2 + H 2 O Pentose-Phosphate pathway Hexose Pentose 2

4 Introduction Carbohydrate metabolism involves a collection of pathways. Glycolysis Hexoses 3-Carbon molecules Gluconeogenesis 3-Carbon molecules Hexoses Fermentation (anaerobic) Citric Acid Cycle (aerobic) Oxidation all the way to CO 2 + H 2 O Pentose-Phosphate pathway Hexose Pentose 2

5 The Glycolytic Reactions There are 10 reactions, which lead from glucose to pyruvate. These reactions couple the lysis (splitting) and oxidation of hexose to the synthesis of 2 ATP s from ADP and P i. Glucose + 2 NAD ADP + 2 P i 2 Pyruvate + 2 NADH + H ATP + 2 H 2 O 3

6 The Glycolytic Reactions There are 10 reactions, which lead from glucose to pyruvate. These reactions couple the lysis (splitting) and oxidation of hexose to the synthesis of 2 ATP s from ADP and P i. Glucose + 2 NAD ADP + 2 P i 2 Pyruvate + 2 NADH + H ATP + 2 H 2 O 3

7 The Glycolytic Reactions There are 10 reactions, which lead from glucose to pyruvate. These reactions couple the lysis (splitting) and oxidation of hexose to the synthesis of 2 ATP s from ADP and P i. Glucose + 2 NAD ADP + 2 P i 2 Pyruvate + 2 NADH + H ATP + 2 H 2 O 3

8 The Glycolytic Reactions There are 10 reactions, which lead from glucose to pyruvate. These reactions couple the lysis (splitting) and oxidation of hexose to the synthesis of 2 ATP s from ADP and P i. Glucose + 2 NAD ADP + 2 P i 2 Pyruvate + 2 NADH + H ATP + 2 H 2 O 3

9 The Glycolytic Reactions There are 10 reactions, which lead from glucose to pyruvate. These reactions couple the lysis (splitting) and oxidation of hexose to the synthesis of 2 ATP s from ADP and P i. Glucose + 2 NAD ADP + 2 P i 2 Pyruvate + 2 NADH + H ATP + 2 H 2 O 3

10 The Glycolytic Reactions There are 10 reactions, which lead from glucose to pyruvate. These reactions couple the lysis (splitting) and oxidation of hexose to the synthesis of 2 ATP s from ADP and P i. Glucose + 2 NAD ADP + 2 P i 2 Pyruvate + 2 NADH + H ATP + 2 H 2 O 3

11 The Glycolytic Reactions There are 10 reactions, which lead from glucose to pyruvate. These reactions couple the lysis (splitting) and oxidation of hexose to the synthesis of 2 ATP s from ADP and P i. Glucose + 2 NAD ADP + 2 P i 2 Pyruvate + 2 NADH + H ATP + 2 H 2 O 3

12 The Glycolytic Reactions There are 10 reactions, which lead from glucose to pyruvate. These reactions couple the lysis (splitting) and oxidation of hexose to the synthesis of 2 ATP s from ADP and P i. Glucose + 2 NAD ADP + 2 P i 2 Pyruvate + 2 NADH + H ATP + 2 H 2 O 3

13 The Glycolytic Reactions There are 10 reactions, which lead from glucose to pyruvate. These reactions couple the lysis (splitting) and oxidation of hexose to the synthesis of 2 ATP s from ADP and P i. Glucose + 2 NAD ADP + 2 P i 2 Pyruvate + 2 NADH + H ATP + 2 H 2 O 3

14 The Glycolytic Reactions Glucose + 2 NAD ADP + 2 P i 2 Pyruvate + 2 NADH + H ATP + 2 H 2 O 4

15 The Glycolytic Reactions Glucose + 2 NAD ADP + 2 P i 2 Pyruvate + 2 NADH + H ATP + 2 H 2 O 5

16 The Glycolytic Reactions 2x Glucose + 2 NAD ADP + 2 P i 2 Pyruvate + 2 NADH + H ATP + 2 H 2 O 5

17 The Glycolytic Reactions Reaction 1: Hexokinase 6

18 The Glycolytic Reactions Clicker Questions: There are four different hexokinase enzymes (I - IV) with differing K M values: Hexokinase K M I, II, III M IV 10-2 M Hexokinase IV, also known as glucokinase, is found in the liver. When the different tissues line up to take glucose from the blood, where is the liver in this lineup? A. First in line B. Last in line C. Somewhere in the middle 7

19 The Glycolytic Reactions Reaction 1: Hexokinase Different tissues have different isoforms of hexokinase. The liver hexokinase, also called glucokinase, has the highest K M, which reflects this organs role in regulating blood glucose levels This reaction has a high negative ΔG. Except for the liver, once phosphorylated, glucose cannot leave the cell. 8

20 The Glycolytic Reactions Reaction 1: Hexokinase 9

21 The Glycolytic Reactions Reaction 1: Hexokinase Problem: What is the standard free energy change, ΔG, for the hexokinase reaction? 9

22 The Glycolytic Reactions Reaction 1: Hexokinase Problem: What is the standard free energy change, ΔG, for the hexokinase reaction? 9

23 The Glycolytic Reactions Reaction 1: Hexokinase 9

24 The Glycolytic Reactions Reaction 2: Glucose 6-Phosphate Isomerase 10

25 The Glycolytic Reactions Reaction 2: Glucose 6-Phosphate Isomerase In the cell, this reaction occurs near equilibrium 11

26 The Glycolytic Reactions Clicker Reaction Questions: 2: Glucose 6-Phosphate If a reaction occurs near equilibrium, what does this say about the actual ΔG for the reaction? Isomerase In the cell, this reaction occurs near equilibrium A. ΔG > 0 B. ΔG 0 C. ΔG < 0 11

27 The Glycolytic Reactions Reaction 3: Phosphofructokinase 1 12

28 The Glycolytic Reactions Reaction 3: Phosphofructokinase 1 12

29 The Glycolytic Reactions Reaction 3: Phosphofructokinase 1 This enzyme catalyzes the first committed step in glycolysis. PFK-1 is regulated by numerous allosteric effectors. 13

30 The Glycolytic Reactions Reaction 4: Aldolase 14

31 The Glycolytic Reactions Reaction 4: Aldolase 14

32 The Glycolytic Reactions Reaction 4: Aldolase 14

33 The Glycolytic Reactions Reaction 4: Aldolase 15

34 The Glycolytic Reactions Reaction 4: Aldolase 15

35 The Glycolytic Reactions Reaction 4: Aldolase 15

36 The Glycolytic Reactions Reaction 5: Triose-Phosphate Isomerase 16

37 The Glycolytic Reactions Reaction 5: Triose-Phosphate Isomerase This reaction also occurs near equlibrium The reaction mechanism involves an endiol intermediate. 17

38 Chemical Modes of Enzymatic Catalysis Acid/Base catalysis Triose phosphate isomerase illustrates both general acid and bases catalysis. Chem 352, Lecture 4 - Part II, Enzyme Catalysis

39 Chemical Modes of Enzymatic Catalysis Acid/Base catalysis Triose phosphate isomerase illustrates both general acid and bases catalysis. Chem 352, Lecture 4 - Part II, Enzyme Catalysis

40 Chemical Modes of Enzymatic Catalysis Acid/Base catalysis Triose phosphate isomerase illustrates both general acid and bases catalysis. Chem 352, Lecture 4 - Part II, Enzyme Catalysis

41 The Glycolytic Reactions Reaction 5: Triose-Phosphate Isomerase 19

42 The Glycolytic Reactions Reaction 5: Triose-Phosphate Isomerase 19

43 The Glycolytic Reactions Reaction 5: Triose-Phosphate Isomerase 19

44 The Glycolytic Reactions Reaction 5: Triose-Phosphate Isomerase This reaction also occurs near equlibrium The reaction mechanism involves an endiol intermediate. 20

45 Chemical Modes of Enzymatic Catalysis Acid/Base catalysis Triose phosphate isomerase illustrates both general acid and bases catalysis. Chem 352, Lecture 4 - Part II, Enzyme Catalysis

46 Chemical Modes of Enzymatic Catalysis Acid/Base catalysis Triose phosphate isomerase illustrates both general acid and bases catalysis. Chem 352, Lecture 4 - Part II, Enzyme Catalysis

47 Chemical Modes of Enzymatic Catalysis Acid/Base catalysis Triose phosphate isomerase illustrates both general acid and bases catalysis. Chem 352, Lecture 4 - Part II, Enzyme Catalysis

48 The Glycolytic Reactions Reaction 6: Glyceraldehyde 3-Phosphate Dehydrogenase 22

49 The Glycolytic Reactions Reaction 6: Glyceraldehyde 3-Phosphate Dehydrogenase This reaction also takes place near equilibrium because the 1,3- bisphosphoglycerate is rapidly depleated. NAD + levels in the cell are typically low, so regeneration of NAD +, is critical for this step in glycolysis. 23

50 The Glycolytic Reactions Reaction 7: Phosphoglycerate Kinase 24

51 The Glycolytic Reactions Reaction 7: Phosphoglycerate Kinase 24

52 The Glycolytic Reactions Reaction 7: Phosphoglycerate Kinase 24

53 The Glycolytic Reactions Reaction 7: Phosphoglycerate Kinase 24

54 The Glycolytic Reactions Reaction 7: Phosphoglycerate Kinase 24

55 The Glycolytic Reactions Reaction 7: Phosphoglycerate Kinase This enzyme is named for the reverse reaction. This reaction is an example of substratelevel phosphorylation. 25

56 The Glycolytic Reactions Reaction 8: Phosphoglycerate Mutase 26

57 The Glycolytic Reactions Reaction 8: Phosphoglycerate Mutase 27

58 The Glycolytic Reactions Reaction 8: Phosphoglycerate Mutase Clicker Questions: What class of reaction is the phosphoglycerate mutase reaction? A. Transferase B. Lyase C. Isomerase D. Ligase E. Oxidoreductase 27

59 The Glycolytic Reactions Reaction 9: Enolase 28

60 The Glycolytic Reactions Reaction 10: Pyruvate Kinase 29

61 The Glycolytic Reactions Reaction 10: Pyruvate Kinase 29

62 The Glycolytic Reactions Reaction 10: Pyruvate Kinase 29

63 The Glycolytic Reactions Reaction 10: Pyruvate Kinase 29

64 The Glycolytic Reactions Reaction 10: Pyruvate Kinase 29

65 The Glycolytic Reactions Reaction 10: Pyruvate Kinase 29

66 The Glycolytic Reactions Reaction 10: Pyruvate Kinase Like phospglycerate kinase, this enzyme is named for the reverse reaction. This reaction is another example of substrate-level phosphorylation. 30

67 The Fates of Pyruvate Pyruvate represents one of the major intersections in metabolism. 31

68 The Fates of Pyruvate Pyruvate represents one of the major intersections in metabolism. Fermentation Citric Acid Cycle Fat 31

69 The Fates of Pyruvate Fermenatation is used to regenerate oxidized NAD + when O 2 cannot be utilized to do this. Fermentation 32

70 The Fates of Pyruvate Fermenatation is used to regenerate oxidized NAD + when O 2 cannot be utilized to do this. Alcohol Fermentation Glycolysis 32

71 The Fates of Pyruvate Fermenatation is used to regenerate oxidized NAD + when O 2 cannot be utilized to do this. Glycolysis Lactate Fermentation Alcohol Fermentation 32

72 Coenzymes and Vitamins (Chapter 7.7) Pyruvate decarboxylase uses the coenzyme thiamine pyrophosphate (TPP). thiamine pyrophosphate is synthesized from vitamin B 1 (thiamine) 33

73 Coenzymes and Vitamins (Chapter 7.7) Pyruvate decarboxylase uses the coenzyme thiamine pyrophosphate (TPP). thiamine pyrophosphate is synthesized from vitamin B 1 (thiamine) 33

74 Coenzymes and Vitamins (Chapter 7.7) Pyruvate decarboxylase uses the coenzyme thiamine pyrophosphate (TPP). thiamine pyrophosphate is synthesized from vitamin B 1 (thiamine) 33

75 Coenzymes and Vitamins (Chapter 7.7) Pyruvate decarboxylase uses the coenzyme thiamine pyrophosphate (TPP). thiamine pyrophosphate is synthesized from vitamin B 1 (thiamine) 33

76 Coenzymes and Vitamins (Chapter 7.7) Pyruvate decarboxylase uses the coenzyme thiamine pyrophosphate (TPP). thiamine pyrophosphate is synthesized from vitamin B 1 (thiamine) 33

77 Coenzymes and Vitamins (Chapter 7.7) Thiamine pyrophosphate is used in many decarboxylation reactions, Including pyruvate decarboxylase 34

78 Coenzymes and Vitamins (Chapter 7.7) Thiamine pyrophosphate is used in many decarboxylation reactions, Including pyruvate decarboxylase 34

79 Coenzymes and Vitamins (Chapter 7.7) Thiamine pyrophosphate is used in many decarboxylation reactions, Including pyruvate decarboxylase 34

80 Free Energy Changes in Glycolysis The overall free energy change is negative. 35

81 Free Energy Changes in Glycolysis The overall free energy change is negative. Sites of Regulation 35

82 Regulation of Glycolysis The enzymes that catalyze the irreversible reactions are the primary sites of allosteric regulation. 36

83 Regulation of Glycolysis The enzymes that catalyze the irreversible reactions are the primary sites of allosteric regulation. 36

84 Regulation of Glycolysis The enzymes that catalyze the irreversible Feedback inhibition reactions are the Things are backing up further down the line primary sites of allosteric regulation. 36

85 Regulation of Glycolysis The enzymes that catalyze the irreversible reactions are the Feed-forward activation Cell needs more ATP primary sites of allosteric regulation. 36

86 Regulation of Glycolysis The enzymes that catalyze the irreversible reactions are the primary sites of allosteric regulation. Feedback inhibition Cell s ATP needs are met Feedback inhibition Cell s ATP needs are met 36

87 Regulation of Glycolysis The enzymes that catalyze the irreversible reactions are the primary sites of allosteric regulation. Feedback inhibition Cell s ATP needs are met Feedback inhibition Cell s ATP needs are met 36

88 Regulation of Glycolysis The enzymes that catalyze the irreversible reactions are the primary sites of allosteric regulation. Feedback inhibition Cell s ATP needs are met Feedback inhibition Cell s ATP needs are met 36

89 Regulation of Glycolysis The enzymes that catalyze the irreversible reactions are the primary sites of allosteric regulation. Feedback inhibition Citric Acid Cycle s needs for anabolic pathways are met 36

90 Regulation of Glycolysis The enzymes that catalyze the irreversible reactions are the primary sites of allosteric regulation. In the Liver: The hormone glucagon is not signaling a need to push glucose out into the blood. 36

91 Regulation of Glycolysis The enzymes that catalyze the irreversible reactions are the primary sites of allosteric regulation. 36

92 Regulation of Glycolysis In the liver, glucokinase, which is livers form of hexokinase, is also regulated by a regulatory protein that binds to glucokinase when fructose 6-phosphate levels are high. 37

93 Regulation of Glycolysis In the liver, glucokinase, which is livers form of hexokinase, is also regulated by a regulatory protein that binds to glucokinase when fructose 6-phosphate levels are high. 37

94 Regulation of Glycolysis In the liver, glucokinase, which is livers form of hexokinase, is also regulated by a regulatory protein that binds to glucokinase when fructose 6-phosphate levels are high. 37

95 Regulation of Glycolysis The liver is the only organ that is able to release glucose back into the blood It does so to satisfy the glucose need of other tissues, particularly the brain. 38

96 Regulation of Glycolysis The liver is the only organ that is able to release glucose back into the blood It does so to satisfy the glucose need of other tissues, particularly the brain. 38

97 Regulation of Glycolysis The liver is the only organ that is able to release glucose back into the blood It does so to satisfy the glucose need of other tissues, particularly the brain. 38

98 Regulation of Glycolysis The liver is the only organ that is able to release glucose back into the blood Under low blood glucose levels, the hormone glucagon signals the liver to halt glycolysis. It does this using a signal transduction pathway. 39

99 Transduction of Extracellular Signals G-Proteins Chem 352, Lecture 6, Part II - Membranes 40

100 Regulation of Glycolysis The liver is the only organ that is able to release glucose back into the blood Under low blood glucose levels, the hormone glucagon signals the liver to halt glycolysis. It does this using a signal transduction pathway. 41

101 Regulation of Glycolysis The liver is the only organ that is able to release glucose back into the blood Under low blood glucose levels, the hormone glucagon signals the liver to halt glycolysis. It does this using a signal transduction pathway. 41

102 Regulation of Glycolysis The liver is the only organ that is able to release glucose back into the blood Under low blood glucose levels, the hormone glucagon signals the liver to halt glycolysis. It does this using a signal transduction pathway. In the Liver: The hormone glucagon is not signaling a need to push glucose out into the blood. 41

103 Regulation of Glycolysis The liver is the only organ that is able to release glucose back into the blood Under low blood glucose levels, the hormone glucagon signals the liver to halt glycolysis. It does this using a signal transduction pathway. 41

104 Regulation of Glycolysis The liver is the only organ that is able to release glucose back into the blood Under low blood glucose levels, the hormone glucagon signals the liver to halt glycolysis. It does this using a signal transduction pathway. 41

105 Regulation of Glycolysis Regulation of Pyruvate Kinase Pyruvate Kinase is also regulated in response to glucagon. Phosphorylation by Protein Kinase A in response to glucagon, lowers its activity. 42

106 Regulation of Glycolysis Regulation of Pyruvate Kinase Pyruvate Kinase is also regulated in response to glucagon. Phosphorylation by Protein Kinase A in response to glucagon, lowers its activity. 42

107 Regulation of Glycolysis Regulation of Pyruvate Kinase Pyruvate Kinase is also regulated in response to glucagon. Phosphorylation by Protein Kinase A in response to glucagon, lowers its activity. 42

108 Regulation of Glycolysis Regulation of Pyruvate Kinase Pyruvate Kinase is also regulated in response to glucagon. Phosphorylation by Protein Kinase A in response to glucagon, lowers its activity. 42

109 Regulation of Glycolysis Regulation of Pyruvate Kinase In addition to being allosterically inhibited by ATP, Pyruvate Kinase is also allosterically activated by frucose 6-phosphate. 43

110 Regulation of Glycolysis Regulation of Pyruvate Kinase In addition to being allosterically inhibited by ATP, Pyruvate Kinase is also allosterically activated by frucose 6-phosphate. 43

111 Regulation of Glycolysis Regulation of Pyruvate Kinase In addition to being allosterically inhibited by ATP, Pyruvate Kinase is also allosterically activated by frucose 6-phosphate. 43

112 Regulation of Glycolysis Regulation of Pyruvate Kinase In addition to being allosterically inhibited by ATP, Pyruvate Kinase is also allosterically activated by frucose 6-phosphate. 43

113 Other Points Skip Section 11.6 Entry of other sugars into glycolysis Skip Section 11.7 Entner-Doudoroff Pathway in Bacteria, which lack PFK-1 44

114 Review of Glycolysis Questions: What is the metabolic purpose behind the glycolytic pathway? 45

115 Review of Glycolysis Questions: What The -lysis the part metabolic of glycolysis purpose means behind to the split. glycolytic Using structural pathway? formulas, draw the chemical equation for the reaction in which a 6-carbon molecule is split into two 3-carbon molecules. 45

116 Review of Glycolysis Questions: What The Even -lysis though the part metabolic the of glycolytic glycolysis purpose pathway means behind to can the split. be glycolytic used Using the structural pathway? formulas, absence of draw oxygen, the chemical there is one equation reaction for in the which reaction and in which oxidation a 6-carbon occurs. molecule is split into two 3-carbon molecules. A. Using structural formulas, draw the chemical equation for this reaction. B. What oxidizing reagent is used in this reaction? 45

117 Review of Glycolysis Questions: What The Even When -lysis though oxygen the part metabolic the is available of glycolytic glycolysis purpose and pathway means can behind to can used, the split. be glycolytic the used Using oxidizing the structural pathway? agent formulas, absence using in the of draw oxygen, previously the chemical there described is one equation reaction for in the is which reoxidized reaction and in by the which oxidation electron a 6-carbon transport occurs. molecule chain. is split into two 3-carbon molecules. A. A. Using structural formulas, show draw the reaction chemical or equation for combination this reaction. or reactions that is used to reoxizide this agent when oxygen cannot be utilized. B. What oxidizing reagent is used in this reaction? B. What is the name of the pathway you drew? 45

118 Next Up Lecture 8 - Carbohydrate Metabolism Part II: Gluconeogenesis, Pentose Phosphate Pathway, and Glycogen Metabolism (Moran et al., Chapter 12) 46