Probiotic in Indonesia : Local issue Indigenous Probiotic Research

Size: px
Start display at page:

Download "Probiotic in Indonesia : Local issue Indigenous Probiotic Research"

Transcription

1 Probiotic in Indonesia : Local issue Indigenous Probiotic Research Ingrid S. Surono SEAMEO TROPMED RCCN University of Indonesia

2 DADIH, TRADITIONAL FERMENTED MILK OF WEST SUMATRA

3 Dadih, Indonesian traditional fermented raw buffalo milk of West Sumatra No heat application on the buffalo milk

4 5 potential dadih strains Lactobacillus plantarum strains IS Lactobacillus plantarum IS Enterococcus faecium strains IS Enterococcus faecium strains IS Enterococcus faecium strains IS-16183

5 Screening studies Viability Adhesion Pathogen inhibition and displacement Immune modulation Detoxification

6 Viability In vivo Sprague Dawley rats and Balb/c mouse L. plantarum IS and IS-20506

7 3D Contour Plot (LACTIC-indeks-1.STA 5v*1810c) Distance Weighted Least Squares C3 C2 VAR3 C1 C0 A0 A1 A2 A above VAR1 Fig 1. Effect on probiotic suplementation on faecal lactic bacteria of Sprague Dawley rats A0 :control; A1 : L.casei Shirota; A2 : L.plantarum IS-10506; A3 : L. plantarum IS-20506

8 Fig 2. Interaction between kind of probiotic and treatment period on Faecal coliform A0 :control; A1 : L.casei Shirota; A2 : L.plantarum IS-10506; A3 : L. plantarum IS-20506

9 Balb/c mice, control, IS dan LGG Rerata jumlah bakteri asam laktat inhibitor TLR2 inhibitor TLR4 tidak ada inhibitor Rerata 1, Jumlah bakteri Coliform Jumlah bakteri E Coli PROBIOTIK LGG PROBIOTIK LIS TANPA PROBIOTIK Kelompok Perlakuan 1.0E-4 PROBIOTIK LGG PROBIOTIK LIS Kelompok Perlakuan TANPA PROBIOTIK A B Fig 3. Bacteriological profile Lactobacillus rhamnosus GG (LGG) group, Lactobacillus plantarum IS (LIS) group in Balb/c induced alergen ovalbumin. A. mean of total fecal lactic acid bacteria afer treatment. LGG and LIS group significantly higher viable count compared to control, at p=0,010 dan p=0,03., respectievely B. Fecal Coliform and Eschericia coli of Balb/c mouse LGG and LIS group has no significant different as compared to control group

10 Viability in Indonesian elderly Enterococcus faecium IS Table 1. Effect of dietary milk and nonfermented milk containing strain IS on fecal coliforms and lactic acid bacteria in elderly subjects Treatments Number of coliforms (log cfu/g) Number of LAB (log cfu/g) Before milk intake (d 0) 5.79 b 6.69 b After milk intake (d 21) 5.91 b 6.49 b After milk containing strain IS intake (d 42) a,b Means in the same column with different superscript letters differ (P<0.05) 6.79 a 7.36 a

11 Adhesion properties of 5 dadih s strains

12 Table 2. Inhibition of pathogen adhesion by LAB strains isolated from dadih fermented milk. Results are shown as average % adhesion inhibition ± SD. % adhesion inhibition 1 Strains Bacteroides vulgatus DSM 1447 Clostridium histolyticum DSM 627 Escherichia coli K2 Salmonella typhimurium ATCC Staphylococcus aureus DSM IS ±11.5* 27.5±9.5* 34.7±10.5* 22.8±2.0* 38.9±6.8* IS ±11.9* 29.5±6.5* 29.5±1.5* 27.2±5.3* 40.1±8.4* IS ±8.8* 29.7±9.4* 34.0±2.8* 24.5±6.3* 37.9±6.3* IS ±0.7* 27.6±8.7* 35.5±0.9* 22.0±0.0* 29.5±7.8* IS ±1.9* 25.5±5.3* 32.7±2.2* 37.6±14.6* 39.4±8.1* 1 Changes in the adhesion of pathogens in the absence of LAB (control) were assigned a value of 0%. * Significantly different from control 0% (p < 0.05).

13 1 Changes in the adhesion of pre-adhered pathogens following the addition of buffer without LAB strains (control) were assigned a value of 0%. * Significantly different from control (p<0.05) Table 3. Displacement of pathogens by LAB strains isolated from dadih fermented milk. Results are shown as average % pathogen displaced ± SD Strains Bacteroide svulgatus DSM 1447 Clostridium histolyticum DSM 627 % displacement pathogen 1 Escherichia coli K2 Salmonella typhimuriu m ATCC Staphylococc us aureus DSM IS ±9.8* 21.7±1.5* 63.8±1.5* 52.3±9.3* 31.0±6.2* IS ±4.9* 22.5±1.9* 61.7±2.7* 61.5±3.1* 20.3±7.9* IS ±8.5* 18.4±4.2* 62.3±0.9* 57.3±15.3* 16.4±7.3 * IS ±5.4* 20.3±8.9* 59.9±2.7* 57.9±9.0* 18.3±8.8* IS ±9.4* 19.3±2.9* 64.6±8.8* 53.3±3.3* 21.5±5.4*

14 Fig 4. Adherence of pathogen strains (B) to human intestinal mucus. Results were expressed average % adhesion to mucus ±SD.

15 Table 4. Adhesion to hydrocarbons of viable and nonviable probiotic and pathogenic strains as measured using the BATH test. Results are expressed as a mean ± standard deviation (SD) (n 4). Pathogen strains % adhesion to hydrocarbons Live (mean±sd) Non-viable (mean±sd) Bacteroides vulgatus DSM ±13.9* 75.6±13.6 Clostridium histolyticum DSM ±1.8* 61.6±4.6 Clostridium difficile DSM ± ±15.2 Escherichia coli K2 32.7±14.5* 70.1±13.8 Enterobacter sakazakii ATCC ±19.7* 78.4±17.5 Staphylococcus aureus DSM ± ±11.34 Probiotic strains Live (mean±sd) % adhesion to hydrocarbons Non-viable (mean±sd) Enterococcus faecium IS Enterococcus faecium IS Enterococcus faecium IS Lactobacillus plantarum IS Lactobacillus plantarum IS ± ± ±10.0* 76.3± ± ± ± ± ± ±8.4 *) significant differences (p<0.05) between hydrophobicity percentages achieved with live and dead cells.

16 Table 5. Percentages of autoaggregation for pathogen (A) and probiotic (B) strains from dadih strains at different times. Control time= 0h. A) Pathogen strains % autoaggregation (20 C) % autoaggregation (37 C) 2h 16 h 20 h 24h 2h 16 h 20 h 24h Bacteroides vulgatus DSM ± ± ± ± ± ± ± ±5.0 Clostridium histolyticum DSM ± ±8.8 * 31.7±5.8 * 63.1±6.9 * 0.2± ± ±2.5 * 58.6±6.2 * Clostridium difficile DSM ± ± ± ± ± ± ± ±5.0 Escherichia coli K2 2.5± ± ± ± ± ± ± ±3.8 Enterobacter sakazakii ATCC ± ± ± ± ± ± ± ±6.0 Staphylococcus aureus DSM ±6.1 * 28.7±1.0 * 29.4±3.3 * 62.4±5.0 * 11.3±0.5 * 31.3±3.7 * 38.3±5.5 * 58.4±6.0 * Probiotics strains 2h % autoaggregation (20 C) 16 h 20 h 24h 2h % autoaggregation (37 C) 16 h 20 h 24h Enterococcus faecium IS ± ± ± ± ± ± ± ±9.0 Enterococcus faecium IS ± ± ± ± ± ± ± ±8.0 Enterococcus faecium IS ±0.5* 1.4±0.5* 14.2± ± ± ± ± ±5.5 Lactobacillus plantarum IS ± ± ± ± ± ± ± ±6.3 Lactobacillus plantarum IS ±0.5* 3.8±2.0* 7.2±2.4* 26.7± ±0.5 * 22.8± ± ±10.0 *) Significant differences among all strains tested in each time

17 BATH (%) Autoaggregation ( % after 20 h incubation) Probiotic strains BATH (mean±sd) Aggregation (mean±sd) Enterococcus faecium IS ± ±5.0 Enterococcus faecium IS ± ±2.3 Enterococcus faecium IS ± ±2.5 Lactobacillus plantarum IS ± ±3.0 Lactobacillus plantarum IS ± ±2.4 Fig 5. Autoaggregation index of the probiotic bacteria isolated from dadih as a function of adhesion of xylene after incubation at room temperature (20 C). Values are the average from at least three experiments.

18 Bacteroides vulgatus Clostridium histolyticum % Coaggregation % coaggregation IS IS IS IS IS IS IS IS IS IS Staphylococcus aureus Enterobacter sakazakii % Coaggregation % Coaggregation IS IS IS IS IS IS IS IS IS IS Fig 6. Coaggregation abilities of probiotic strains with pathogens after 2h incubation at 20ºC expressed as percentages. Values are the average ±SD from at least three experiments.

19 Clostridium difficile Escherichia coli % Coaggregation % Coaggregation IS IS IS IS IS IS IS IS IS IS Fig 6. Coaggregation abilities of probiotic strains with pathogens after 2h incubation at 20ºC expressed as percentages. Values are the average ±SD from at least three experiments.

20 Immune modulation In vivo studies (Sprague Dawley rats and Balb/c mouse)

21 Effect of Probiotic on faecal siga in Sprague Dawley Rat FAECAL ANAEROBIC BACTERIA Delta OD days 0-7 days 0-10 days A1B0 A2B0 A3B0 Fig 7. Effect of Probiotic on faecal siga in rats A1 : L. casei Shirota; A2 : L. plantarum IS-10506; A3 : L plantarum IS-20506

22 E. faecium IS in Young children (under 5 years) Serum IgA siga saliva Serum IgG Nutritional status

23 IgG serum total konsentrasi IgG (mg/ml) Awal Akhir kontrol probiotik Delta IgG serum probiotic group (0.94 mg/ml) > control group (0.569 mg/ml) kenaikan konsentrasi total IgG serum (mg/ml) normal underweight underweight berat The highest increment in normal nutritional status children

24 IgA serum total based on nutritional status Delta IgA serum total (mg/ml) normal plasebo Placebo undernourished Nutritional Status pro Probiotic tik Severe undernourished IgA serum normal > undernourished> severe undernourished E. faecium IS effective in increasing IgA serum total in undernourished children

25 IgA SALIVA TOTAL Concentration (mg/ml) IgA Saliva Total ** hari ke-0 ** placebo Treatment ** ** probiotik Probiotic hari ke-90 Day 0 Day 90 **Significant at α = 0.05

26 siga SALIVA TOTAL BASED ON NUTRITIONAL STATUS Delta IgA saliva total (mg/ml) Normal normal Undernourished kurang sangat Severe kurang undernourished Nutritional Status Status Gizi Placebo plasebo Probiotic probiotik siga undernourished > severe undernourished > normal E. faecium IS more effective in increasing siga in undernourished and severe undernourished children

27 Body weight (linear regression) Body weight (kg) y2 = 0.105x R 2 = y1 = x R 2 = Minggu ke- placebo probiotik Linear (placebo) Linear (probiotik) Slope (b) = PBB, b2 (probiotic) = 1.5 b1 (placebo) Increase body weight in probiotic group tends to be higher than placebo, 1.5 times

28 persen (%) Probiotik Kontrol Fig 12. Percentage of children with improved nutritional status after probiotic treatment Table 3. Percentage of children with improved nutritional status Nutritional status improvement % subject who improved nutritional status Control Probiotic SevereUnderweight to underweight Severe Underweight to normal Underweight to normal

29 Detoxification Bio elimination of cyanobacterial toxin Microcystin-LR (MC-LR) Metabolically active removal of MC-LR

30 Cyanobacteria, blue-green algae typical for eutrophic water bodies toxicity: hepatotoxins (liver toxins), tumour promoters, inhibit protein phosphatases 1 and 2A, acute LD 50 (mouse, i.p.) ca µg/kg depending on the toxin variant, oral toxicity weaker (lethal doses ca times higher Microcystin-LR (6) D-Glu (iso) (7) N-methyldehydroAla COOH (5) Adda HN OCH 3 H 3 C O NH H CH 3 CH 3 H O N HN C N O CH 3 O N NH O (1) D-Ala CH 2 H 3 C O CH 3 HN CH 3 H N CH 3 O COOH (2) L-Leu NH 2 (4) L-Arg (3) D-erythro-β-methylAsp (iso) WHO guideline value: max. 1 µg/l microcystin-lr in drinking water

31 Fig MC-LR Removal percentage of the best two strains IS and IS-2050 compared to commercial Shirota strain (freeze dried cultures)

32 MC-LR removed (%) b c d Incubation time (h) c a d c d MC-LR removed (%) b d d Incubation time (h) a c e Fig. 5a Microcystin-LR removal percentage of viable (black), heat-treated (grey), and acid treated (white) cells of Lactobacillus plantarum IS-10506, 8.6x x cfu per assay at ph 7, 22 o C. Error bars show the SD of the mean of three experiments. Bars with different letter are significantly different at 95 % confidence level Fig. 5b Microcystin-LR removal percentage of viable (black), heat-treated (grey), and acid treated (white) cells of Lactobacillus plantarum IS-20506, 7.6x x cfu per assay at ph 7, 22 o C. Error bars show the SD of the mean of three experiments. Bars with different letter are significantly different at 95 % confidence level

33 MC-LR removed (%) MC-LR rem oved (% ) incubation time (h) incubation time (h) Fig. 6a Microcystin-LR removal percentages by viable cells of Lactobacillus plantarum IS at ph 7, White bars (22 o C) and Grey bars (37 o C). Error bars indicate confidence intervals at 95% level. Fig.6b Microcystin-LR removal percentages of viable cells of Lactobacillus plantarum IS at ph 7,White bars (22 o C) and Grey bars (37 o C). Error bars indicate confidence intervals at 95% level.

34 IS IS MC-LR removed (%) MC-LR removed (%) 9 60 log viable cells/ml log viable cells/ml Incubation time (h) Incubation time (h) 7 Figure 7a. MC-LR removed (%) and log viable cells ml -1 of IS strain at different glucose concentration during incubation at 37 C. Empty legends stand for MC-LR removed, and filled legend for log viable cells. Symbols (, ) 0% of glucose, (, ) to 1% of glucose, (, ) 2% of glucose and (, ) 3% of glucose. Figure 7b. MC-LR removed (%) and log viable cells ml -1 of IS strain at different glucose concentration during incubation at 37 C. Empty legends stand for MC-LR removed, and filled legend for log viable cells. Symbols (, ) 0% of glucose, (, ) to 1% of glucose, (, ) 2% of glucose and (, ) 3% of glucose.

35 MC-LR removed (%) E E E E E E+13 bacterial concentration (CFU ml -1 ) L. rhamnosus GG L. rhamnosus LC-705 B. longum 46 B. lactis 420 B. lactis Bb12 L. plantarum Lp-115 L. plantarum IS L. plantarum IS Figure 8. Effect of bacterial concentration on removal of MC-LR. Initial MC-LR concentration 100 μg L -1, incubation time 24 h, temperature 37 C, average ± SD, n=3.

36 On going research L. plantarum IS : HIV/AIDS patient Profile fecal microbiota Humoral immune system Cellular immune system

37 Published papers 1. Collado, M. C., Surono, I.S., Meriluoto, J., Salminen, S., Potential Probiotic Characteristics of Lactobacillus and Enterococcus Strains Isolated from Traditional Dadih Fermented Milk against Pathogen Intestinal Colonization. Journal of Food Protection Vol.70, Collado, M. C., Surono, I., Meriluoto, J., Salminen, S., Indigenous Dadih Lactic Acid Bacteria: Cell- Surface Properties and Interaction with Pathogen. Journal of Food Science, vol. 72, Surono, I.S., Collado, M.C., Salminen, S. and Meriluoto, J. Effect of glucose and incubation temperature on metabolically active Lactobacillus plantarum from dadih in removing microcystin-lr. Food and Chemical Toxicology (in communication) 4. Usman Pato, Ingrid S. Surono, Koesnandar, Akiyoshi Hosono, Hypo-cholesterolemic Effect of Indigenous Dadih Lactic Acid Bacteria by Deconjugation of Bile Salts. Asian-Aust. J. Anim. Sci., Vol. 17 No. 12 : Jorry Dharmawan, Ingrid S. Surono, Lee Yuan Kun, Adhesion Properties of Indigenous Dadih Lactic Acid Bacteria on Human Intestinal Mucosal Surface Asian-Aust. J. Anim. Sci., Vol. 19 No. 5 : Surono, I.S., In vitro Probiotic properties of indigenous dadih lactic bacteria. Asian-Aust. J. of Anim. Sci. 16, 5,

38 Financial Support Viability, and immuno modulatory properties : Ministry of Research and Technology ( ) Adhesion and detoxification properties : Academy of Finland decisions RC and RC

39 Prebiotic Research Roots and tubers as source of prebiotic : Sweet potato, bread fruit, taro, araroot

40 Government Regulation BADAN POM (Indonesian FDA) Probiotic as functional foods Claim of Nutrition fact : Enriched, fortified : contain probiotic bacteria more than minimum dose 10 6 cfu/day viable count until expired date Containing : probiotic more than minimum dose 10 7 cfu/day Rich in probiotic : more than minimum dose 10 8 cfu/day List of Probiotic bacteria?

41 Nutritient function claim : Probiotic (Lactobacillus and Bifidobacterium, expect L. bulgaricus) can help in maintaining intestinal function) Confirmation of the presence of probiotic bacteria? Bifidobacteria : can be produced in home industry?

42 Health benefit claim No health claim in Label and advertisement Label and advertisement for probiotic product should mention Genus, species, and strain, minimun viable counts at the end of expired date, information about amount of probiotic and its fisiological effect. Storage condition, contact number of producer Human studies on Indonesian people, minimum 20 subjects : Adequate amount?

43 Prebiotic as functional foods Enriched, fortified (at least 10% from daily value 10g/day) Containing : % from daily value (10g/day) per serving Rich in prebiotic, very good source : at least 20 % of DV per serving No nutrient function claims in label and advertisement No helath claim in label and advertisement

44 Consumer Perspective Lack of knowledge about probiotic and prebiotic : Street vendor sells probiotic product without refrigerator Street vendor sells yogurt and claim as yogurt probiotic Concern about the viable counts in probiotic product (real cfu/ml or g) Relatively expensive

45 Street vendor sells Home made yogurt and claim as yogurt probiotic Yogurt probiotic (Yogurt for health)

46 Producers Perspective Rigid government regulations for health claim : using defined commercial strain with scientific based evident, can not do health claim Need to educate customer to gain awareness on health benefit of probiotic

47 High Appreciation to Wonderful Team work

48

49 Thank You Very Much for your kind attention

Indigenous Probiotics and Immunological Effects Ingrid S Surono Food Technology Department, Faculty of Engineering-BINUS University,

Indigenous Probiotics and Immunological Effects Ingrid S Surono Food Technology Department, Faculty of Engineering-BINUS University, Indigenous Probiotics and Immunological Effects Ingrid S Surono Food Technology Department, Faculty of Engineering-BINUS University, Indonesian Scientific Society for Probiotics and Prebiotics (ISSPP)

More information

Probiotics for Aquaculture

Probiotics for Aquaculture Probiotics for Aquaculture Seppo Salminen Functional Foods Forum University of Turku Finland Acquaculture Fish farming Carp and related fish are the most farmed fish (mainly in Asia) at 18,5 million metric

More information

Role of commercial probiotic strains against human pathogen adhesion to intestinal mucus

Role of commercial probiotic strains against human pathogen adhesion to intestinal mucus Letters in Applied Microbiology ISSN 0266-8254 ORIGINAL ARTICLE Role of commercial probiotic strains against human pathogen adhesion to intestinal mucus M.C. Collado 1, J. Meriluoto 2 and S. Salminen 1

More information

ABSTRACT MATERIALS AND METHODS M. CARMEN COLLADO, 1 * INGRID S. SURONO, 1,2 JUSSI MERILUOTO, 3 AND SEPPO SALMINEN 1

ABSTRACT MATERIALS AND METHODS M. CARMEN COLLADO, 1 * INGRID S. SURONO, 1,2 JUSSI MERILUOTO, 3 AND SEPPO SALMINEN 1 700 Journal of Food Protection, Vol. 70, No. 3, 2007, Pages 700 705 Copyright, International Association for Food Protection Potential Probiotic Characteristics of Lactobacillus and Enterococcus Strains

More information

3 Aggregation and adhesion abilities to HCT-116 cellsof probiotic candidates Lactobacillus plantarum

3 Aggregation and adhesion abilities to HCT-116 cellsof probiotic candidates Lactobacillus plantarum 25 3 Aggregation and adhesion abilities to HCT-116 cellsof probiotic candidates Lactobacillus plantarum strains isolated from mandai,indonesian fermented food against enteropathogens 2 Abstract Ten probiotic

More information

2/3/2011. Adhesion of Bifidobacterium lactis HN019 to human intestinal

2/3/2011. Adhesion of Bifidobacterium lactis HN019 to human intestinal PROBIOTICS LEARNING THE WHY AND WHEN PROBIOTICS DEFINITION live micro-organisms organisms that are beneficial to the host organism WHO: Live organisms which, when administered in adequate amounts, confer

More information

Issue 3, September usio

Issue 3, September usio Issue 3, September 2014 usio 1 Lactobacillus casei strain Shirota Research Update IMPROVEMENT IN INTESTINAL HEALTH 1. A probiotic fermented milk drink containing Lactobacillus casei strain Shirota improves

More information

International Food Research Journal 23(5): (2016) Journal homepage:

International Food Research Journal 23(5): (2016) Journal homepage: International Food Research Journal 23(5): 2234-2240 (2016) Journal homepage: http://www.ifrj.upm.edu.my Aggregation and adhesion abilities to enterocyte-like HCT-116 cells of probiotic candidates Lactobacillus

More information

השפעת חיידקים פרוביוטיים

השפעת חיידקים פרוביוטיים השפעת חיידקים פרוביוטיים החיים בחלל )המעי(... על רון שאול יחידת גסטרו ילדים מרכז רפואי רמב"ם Introduction The intestinal microflora primarily in the large bowel consists mostly on benign bacterial species

More information

Survival of new probiotic strains with anti-inflammatory & anti-obesity effects used in non-fat yogurt and low-fat Cheddar cheese making

Survival of new probiotic strains with anti-inflammatory & anti-obesity effects used in non-fat yogurt and low-fat Cheddar cheese making Survival of new probiotic strains with anti-inflammatory & anti-obesity effects used in non-fat yogurt and low-fat Cheddar cheese making Veronique Demers-Mathieu, Ph.D. Department of Microbiology & Medicine

More information

Role of Food Matrix for Probiotic Effects

Role of Food Matrix for Probiotic Effects Role of Food Matrix for Probiotic Effects W. Kneifel Department of Food Science and Technology BOKU University of Natural Resources and Life Sciences Vienna Hohenheim, 15 Oct.. 2010 wolfgang.kneifel@boku.ac.at

More information

Probiotics in Pediatric Health. AANP Annual Convention

Probiotics in Pediatric Health. AANP Annual Convention Probiotics in Pediatric Health AANP Annual Convention Don Brown, ND July 12, 2017 Human Microbiome Richness and Metabolic Markers Human gut microbial composition was studied in 292 Danish adults Individuals

More information

Hypocholesterolemic Effect of Indigenous Dadih Lactic Acid Bacteria by Deconjugation of Bile Salts*

Hypocholesterolemic Effect of Indigenous Dadih Lactic Acid Bacteria by Deconjugation of Bile Salts* 74 Hypocholesterolemic Effect of Indigenous Dadih Lactic Acid Bacteria by Deconjugation of Bile Salts* Usman Pato**, Ingrid S. Surono, Koesnandar and Akiyoshi Hosono Faculty of Agriculture, Riau University,

More information

Bile and Acid Tolerance of Lactic Acid Bacteria Isolated from Dadih and Their Antimutagenicity against Mutagenic Heated Tauco*

Bile and Acid Tolerance of Lactic Acid Bacteria Isolated from Dadih and Their Antimutagenicity against Mutagenic Heated Tauco* 1680 Bile and Acid Tolerance of Lactic Acid Bacteria Isolated from Dadih and Their Antimutagenicity against Mutagenic Heated Tauco* Usman Pato** Faculty of Agriculture, University of Riau, Pekanbaru 28293,

More information

Effect of Feed Supplementation of Lactic Acid Bacteria on Microbial Changes in Broiler Intestine

Effect of Feed Supplementation of Lactic Acid Bacteria on Microbial Changes in Broiler Intestine Kasetsart J. (Nat. Sci.) 42 : 269-276 (2008) Effect of Feed Supplementation of Lactic Acid Bacteria on Microbial Changes in Broiler Intestine Patoomporn Chim-anage 1 *, Varaporn Hirunvong 1, Pramote Sirirote

More information

Probiotics: Targeting obesity

Probiotics: Targeting obesity Probiotics: Targeting obesity Azadeh Nadjarzadeh, PhD in Nutrition Nutrition and Food Security Research Centre, Shahid Sadoughi University of Medical Sciences, Yazd, Iran azadehnajarzadeh@gmail.com Among

More information

Understanding probiotics and health

Understanding probiotics and health Understanding probiotics and health Gemma Laws MSc Student Microbiology and Immunology Department The gut microbiota The name given to the total microbial population living in our intestine Bacteria, fungi,

More information

Study summaries L. casei 431

Study summaries L. casei 431 This binder provides you with summaries of selected publications on Lactobacillus paracasei subsp. paracasei L. casei 431. The publications are clinical studies performed in humans documenting the effects

More information

Understanding Today s Probiotics Regulations in South East Asia. Wai Mun Poon Regulatory Affairs Consultant

Understanding Today s Probiotics Regulations in South East Asia. Wai Mun Poon Regulatory Affairs Consultant Understanding Today s Probiotics Regulations in South East Asia Wai Mun Poon Regulatory Affairs Consultant waimun@wongsjasia.com Probiotic Foods in South East Asia More probiotic foods are being introduced

More information

EFFECTS OF ALETA IN PROMOTING THE GROWTH OF PROBIOTIC BACTERIA: IN VITRO STUDY

EFFECTS OF ALETA IN PROMOTING THE GROWTH OF PROBIOTIC BACTERIA: IN VITRO STUDY 2 Senoko Drive 758 200 Singapore tel: +65.6755.633 www.kemin.com EFFECTS OF ALETA IN PROMOTING THE GROWTH OF PROBIOTIC BACTERIA: IN VITRO STUDY Lakshmibai Vasanthakumari Bindhu. Ph.D Abstract: It is well

More information

Probiotics : What we Know and Where we are Going Next

Probiotics : What we Know and Where we are Going Next Probiotics : What we Know and Where we are Going Next Neerja Hajela, Ph.D. General Manager - Science and Regulatory Affairs Yakult Danone India Pvt. Ltd. Functional Food Market Probiotics an important

More information

Prebiotics, probiotics and synbiotics: An update

Prebiotics, probiotics and synbiotics: An update Prebiotics, probiotics and synbiotics: An update Natalie Schellack Professor, School of Pharmacy, Sefako Makgatho Health Sciences University Yolanda Combrinck Former academic intern, School of Pharmacy,

More information

Bovine mastitis may be associated with the deprivation of gut Lactobacillus. C. Ma, J. Zhao, X. Xi, J. Ding, H. Wang, H. Zhang and L.Y.

Bovine mastitis may be associated with the deprivation of gut Lactobacillus. C. Ma, J. Zhao, X. Xi, J. Ding, H. Wang, H. Zhang and L.Y. Supplementary online material of Beneficial Microbes DOI: http://dx.doi.org/10.3920/bm2015.0048. Bovine mastitis may be associated with the deprivation of gut Lactobacillus C. Ma, J. Zhao, X. Xi, J. Ding,

More information

The impact of the microbiome on brain and cognitive development

The impact of the microbiome on brain and cognitive development The Gut-Brain Axis The impact of the microbiome on brain and cognitive development Diane Stadler, PhD, RD Oregon Health & Sciences University, Portland, Oregon Lao-American Nutrition Institute With acknowledgements

More information

Manipulating the gut microbiome

Manipulating the gut microbiome Manipulating the gut microbiome William DePaolo, PhD Associate Professor Medicine Director Center for Microbiome Sciences & Therapeutics University of Washington Microbiota The actual bugs that reside

More information

Prevention and Therapy of Antibiotic Associated Diarrhea (ADD) through Probiotics

Prevention and Therapy of Antibiotic Associated Diarrhea (ADD) through Probiotics Prevention and Therapy of Antibiotic Associated Diarrhea (ADD) through Probiotics DGMIM, 15.-16.10.2010 16.10.2010 Stuttgart Prof. Rémy Meier, MD GI-Department University Hospital Liestal, Switzerland

More information

ESTABLISHMENT AND DEVELOPMENT OF LACTIC ACID BACTERIA AND BIFIDOBACTERIA MICROBIOTA IN BREAST-MILK AND THE INFANT GUT

ESTABLISHMENT AND DEVELOPMENT OF LACTIC ACID BACTERIA AND BIFIDOBACTERIA MICROBIOTA IN BREAST-MILK AND THE INFANT GUT Text 1 ESTABLISHMENT AND DEVELOPMENT OF LACTIC ACID BACTERIA AND BIFIDOBACTERIA MICROBIOTA IN BREAST-MILK AND THE INFANT GUT Solís G. a, de los Reyes-Gavilan C.G. b, Fernández N. a, Margolles A. b and

More information

Examining the effects of pre and probiotics on gut microbiota during the ageing process

Examining the effects of pre and probiotics on gut microbiota during the ageing process Session: Reviewing key ingredients shaping nutrition for healthy ageing Tuesday 22 nd November 2016 Examining the effects of pre and probiotics on gut microbiota during the ageing process Louise R Wilson

More information

Viability of Probiotic Bacteria during Refrigerated Storage of Commercial Probiotic Fermented Dairy Products Marketed In Jordan

Viability of Probiotic Bacteria during Refrigerated Storage of Commercial Probiotic Fermented Dairy Products Marketed In Jordan Journal of Food Research; Vol. 6, No. 2; 207 ISSN 927-0887 E-ISSN 927-0895 Published by Canadian Center of Science and Education Viability of Probiotic Bacteria during Refrigerated Storage of Commercial

More information

More than a gut feeling: How the microbiota improves health

More than a gut feeling: How the microbiota improves health More than a gut feeling: How the microbiota improves health Amanda Ramer-Tait, PhD Harold and Esther Edgerton Assistant Professor of Immunology and Microbiology Department of Food Science, University of

More information

NEERJA HAJELA, PhD Head Science Yakult Danone India Pvt. Ltd.

NEERJA HAJELA, PhD Head Science Yakult Danone India Pvt. Ltd. NEERJA HAJELA, PhD Head Science Yakult Danone India Pvt. Ltd. What we already know. Functional Foods - foods that provide a health benefit beyond the traditional nutrients it contains.. American Dietetics

More information

Microencapsulation of Probiotics

Microencapsulation of Probiotics Microencapsulation of Probiotics Brian Carpenter Advanced BioNutrition Corp. 7155 Columbia Gateway Drive Columbia, MD 21046 USA bcarpenter@abn-corp.com Institute of Food Technologists 1 Outline Probiotic

More information

Discussion Group 1: Where pathogenesis and commensalism meet

Discussion Group 1: Where pathogenesis and commensalism meet Discussion Group 1: Where pathogenesis and commensalism meet Pathogen Probiotic Shared Challenges: Survive gastric juice Survive bile Virulence factors Competition Survival and growth Attachment Evasion

More information

Vimta Labs Ltd., Life Sciences Facility, Plot No. 5, Alexandria Knowledge Park, Genome Valley, Shameerpet, Hyderabad, Telangana

Vimta Labs Ltd., Life Sciences Facility, Plot No. 5, Alexandria Knowledge Park, Genome Valley, Shameerpet, Hyderabad, Telangana Last Amended on - Page 1 of 14 I. DRUGS & PHARMACEUTICALS 1. Biological Assays Antibiotics And Other Drugs Bulk Drugs & Their Formulations: Erythromycin, Gentamicin, Nystatin IP Appendix 9.1 2.2.10 BP

More information

Probiotic action and health and well-being of children. Seppo Salminen Functional Foods Forum Finland

Probiotic action and health and well-being of children. Seppo Salminen Functional Foods Forum Finland Probiotic action and health and well-being of children Seppo Salminen Functional Foods Forum Finland DEFINITION OF A PROBIOTIC Probiotic:...a living microbial preparation, which beneficially influences

More information

FIBER HEALTH BENEFITS

FIBER HEALTH BENEFITS FIBER FIBER HEALTH BENEFITS Normal Laxation: Increase stool weight from fiber, water retained by fiber and bacterial mass Eases defecation and prevents or relieves constipation Cereal fibers are best;

More information

Seppo Salminen Mimi Tang

Seppo Salminen Mimi Tang Koletzko B. (ed): Pediatric Nutrition in Practice. Basel, Karger, 2008, pp 80 84 1 General Aspects of Childhood Nutrition 1.8 Gut Microbiota in Infants Seppo Salminen Mimi Tang Key Words M i c r o b i

More information

Diarrhea is a major cause of child mortality

Diarrhea is a major cause of child mortality Paediatrica Indonesiana VOLUME 54 January NUMBER 1 Original Article Probiotic Weisella paramesenteroides on enteropathogenic E. coli-induced diarrhea Aslinar, Yusri Dianne Jurnalis, Endang Purwati RN,

More information

Best use of a probiotic supplement (Symprove TM )

Best use of a probiotic supplement (Symprove TM ) UCL School of Pharmacy You ve got to be in it to win it Best use of a probiotic supplement (Symprove TM ) Professor Simon Gaisford s.gaisford@ucl.ac.uk @sgaisforducl Probiotics Probiotic market estimated

More information

Probiotics and Prebiotics: Frequently Asked Questions

Probiotics and Prebiotics: Frequently Asked Questions 1. What are probiotics? Our intestines contain over 100 trillion bacteria. This is 1,000 times the number of cells that make up the entire human body. Many of these bacteria play an important role in protecting

More information

Properties of Evidence-Based Probiotics for Human Health

Properties of Evidence-Based Probiotics for Human Health DK3341_book.fm Page 109 Wednesday, July 27, 2005 8:26 AM 5 Properties of Evidence-Based Probiotics for Human Health Lynne V. McFarland and Gary W. Elmer CONTENTS 5.1 Introduction... 110 5.2 Desirable Properties

More information

The science behind probiotics Colin Hill, APC Microbiome Institute University College Cork Ireland

The science behind probiotics Colin Hill, APC Microbiome Institute University College Cork Ireland The science behind probiotics Colin Hill, APC Microbiome Institute University College Cork Ireland Illustration Charis Tsevis Probiotics live microorganisms that, when administered in adequate amounts,

More information

SYNBIOTIC EFFECTS DO THEY MAKE A DIFFERENCE?

SYNBIOTIC EFFECTS DO THEY MAKE A DIFFERENCE? SYNBIOTIC EFFECTS DO THEY MAKE A DIFFERENCE? Gail Cresci, PhD, RD, LD, CNSC Associate Staff Departments of Gastroenterology/Nutrition/Pathobiology Cleveland Clinic Cleveland, OH Objectives Discuss rationale

More information

Lactobacillus salivarius Ls-33

Lactobacillus salivarius Ls-33 TM 52-1e Lactobacillus salivarius Ls-33 CHAACTEISTICS OF THE SPECIES Lactobacillus salivarius is a Gram-positive, non-spore forming, homofermentative rod and is a common inhabitant of the human intestinal

More information

Lavanya Nutankalva,MD Consultant: Infectious Diseases

Lavanya Nutankalva,MD Consultant: Infectious Diseases Lavanya Nutankalva,MD Consultant: Infectious Diseases Introduction The word Probiotic was derived from the Greek phrase meaning for life." was first coined in the 1960s by Lilly and Stillwell. Probiotics

More information

The role of nutrition in optimum gastrointestinal health

The role of nutrition in optimum gastrointestinal health The role of nutrition in optimum gastrointestinal health Kelly A. Tappenden, Ph.D., R.D., FASPEN Kraft Foods Human Nutrition Endowed Professor University Distinguished Teacher-Scholar University of Illinois

More information

Increasing barrier function with multispecies probiotics

Increasing barrier function with multispecies probiotics Increasing barrier function with multispecies probiotics Elsbeth Pekelharing, MSc Science Liaison We strive for the most effective microbiome management solutions with our evidence-based & indication-specific

More information

INDEX. THT company history Your bacteria producer Probiotics - pure culture Probiotics - formulations and finished products...

INDEX. THT company history Your bacteria producer Probiotics - pure culture Probiotics - formulations and finished products... 1 INDEX THT company history... 03 Your bacteria producer... 04 Probiotics - pure culture... 05 Probiotics - formulations and finished products... 06 Packaging services... 06 Tailor made... 07 Prolactys...

More information

Probiotics for Disease Prevention and Amelioration. Neerja Hajela, PhD Yakult Danone India Pvt. Ltd.

Probiotics for Disease Prevention and Amelioration. Neerja Hajela, PhD Yakult Danone India Pvt. Ltd. Probiotics for Disease Prevention and Amelioration Neerja Hajela, PhD Yakult Danone India Pvt. Ltd. Microbiology turns inwards Human Metagenome project and Meta Hit project reveals that the gut micro flora

More information

GUT MICROBIOME WHAT IS IT? WHY IS IT IMPORTANT FOR HUMAN HEALTH?

GUT MICROBIOME WHAT IS IT? WHY IS IT IMPORTANT FOR HUMAN HEALTH? GUT MICROBIOME WHAT IS IT? WHY IS IT IMPORTANT FOR HUMAN HEALTH? Corrie Whisner, PhD School of Nutrition and Health Promotion Arizona State University Center for Research on Ingredient Safety Annual Meeting

More information

Choosing the Right Probiotic

Choosing the Right Probiotic Choosing the Right Probiotic Robert Dadd Master Herbalist : Flora Health Canada What is the Microbiome? The community of microorganisms that inhabits our bodies Skin, mouth, nose, digestive tract, and

More information

Table S1. Overview of most studies on impact of ingested bacteria on human gut microbiota using methodologies not based on sequencing approaches

Table S1. Overview of most studies on impact of ingested bacteria on human gut microbiota using methodologies not based on sequencing approaches Supplementary Material Fate, activity, and impact of ingested bacteria within the human gut microbiota Muriel Derrien and Johan E.T. van Hylckama Vlieg Danone Nutricia Research, RD 128 Avenue de la Vauve,

More information

INTESTINAL MICROBIOTA EXAMPLES OF INDIVIDUAL ANALYSES

INTESTINAL MICROBIOTA EXAMPLES OF INDIVIDUAL ANALYSES EXAMPLES OF INDIVIDUAL ANALYSES INTESTINAL MICROBIOTA Microbiota in the animal or human intestine has evolved together with the host. Consequently, the gastrointestinal tract could be considered a metacommunity,

More information

Dysbiosis & Inflammation

Dysbiosis & Inflammation MASTERING THE MICROBIOME: Dysbiosis & Inflammation 2017 Tom Fabian, PhD It is reasonable to propose that the composition of the microbiome and its activities are involved in most, if not all, of the biological

More information

malaysia Volume 4 October / November / December 2015 grow bacteria eat healthy KDN: PP 17575/03/2013 (031985)

malaysia Volume 4 October / November / December 2015 grow bacteria eat healthy KDN: PP 17575/03/2013 (031985) malaysia Volume 4 October / November / December 2015 grow Immunity-fighting bacteria eat healthy look healthy KDN: PP 17575/03/2013 (031985) contents Publisher: UHS Essential Health (Malaysia) Sdn. Bhd.

More information

Azienda Ospedaliera S. Camillo Forlanini. Unità Operativa di Gastroenterologia. Moscow June Cosimo Prantera

Azienda Ospedaliera S. Camillo Forlanini. Unità Operativa di Gastroenterologia. Moscow June Cosimo Prantera / Azienda Ospedaliera S. Camillo Forlanini Unità Operativa di Gastroenterologia Moscow June 2006 Cosimo Prantera ANTIBIOTICS AND BACTERIAL SPECIES Metronidazole Bacteroides - Clostridia Ciprofloxacin Escherichia

More information

AUTOIMMUNITY AND THE GUT

AUTOIMMUNITY AND THE GUT AUTOIMMUNITY AND THE GUT Autoimmunity The Past The rationale - All about a malfunctioning immune system The immune system is overactive to the point of attacking the body Avoid anything that stimulates

More information

ROLE OF THE GUT BACTERIA

ROLE OF THE GUT BACTERIA ROLE OF THE GUT BACTERIA Our Good Bacteria In a perfect world, we would all have a proper ratio of good bacteria And what could this proper ratio do for us? The knowledge of the connections between our

More information

VITAMINS, MINERALS AND THE GUT

VITAMINS, MINERALS AND THE GUT VITAMINS, MINERALS AND THE GUT Nutrients Looking at individual nutrients that are involved with gut health can be misleading This is not about taking individual nutrients It supports more a whole food

More information

HMP is lead by NIH but is an International Consortium.

HMP is lead by NIH but is an International Consortium. Human Microbiome The Human Microbiome Project was one of the spin offs of the Human Genome Project. The HMP project started in 2007 with the overall goal of Characterizing the Human Microbiome and determine

More information

ADJUSTING SPECIAL DIET STYLES

ADJUSTING SPECIAL DIET STYLES Slide 1 ADJUSTING SPECIAL DIET STYLES Slide 2 Diet and Microflora Alterations in diet can account for 57% of structural changes in the gut bacteria (genetics only account for 12%) Western diet high fat,

More information

Effects of probiotics in the treatment of alcoholic hepatitis: randomized controlled multicenter study

Effects of probiotics in the treatment of alcoholic hepatitis: randomized controlled multicenter study Effects of probiotics in the treatment of alcoholic hepatitis: randomized controlled multicenter study Lactobacillus subtilis/streptococcus faecium Lactobacillus rhamnosus R0011/acidophilus R0052 Ki Tae

More information

The Gut Microbiome: 101 Justin Carlson University of Minnesota

The Gut Microbiome: 101 Justin Carlson University of Minnesota The Gut Microbiome: 101 Justin Carlson University of Minnesota Where are we now? 360 B.C. 2003 Human Gut Microbes Associated With Obesity Ley et al., Nature. 2006. Consumer Driven Science For Better of

More information

9/18/2018. The Physiological Roles of the Intestinal Microbiota. Learning Objectives

9/18/2018. The Physiological Roles of the Intestinal Microbiota. Learning Objectives The Physiological Roles of the Intestinal Microbiota Kelly A. Tappenden, Ph.D., R.D., FASPEN Professor and Head, Kinesiology and Nutrition University Distinguished Teacher-Scholar University of Illinois

More information

Next generation of probiotics

Next generation of probiotics Session: Evaluating next generation ingredients to support digestive health Wednesday 23 rd November 2016 Next generation of probiotics Louise R Wilson RD PhD Assistant Science Manager, Yakult UK Ltd LWilson@yakult.co.uk

More information

How probiotics could prevent iron deficiency in a new way

How probiotics could prevent iron deficiency in a new way Probiotics Congress: Asia How probiotics could prevent iron deficiency in a new way 29th Feb, 2016 Radhakrishna Hemmad Regional Director Asia Pacific Probi AB Agenda Short introduction to Probi Iron deficiency:

More information

!Microbiology Profile, stool

!Microbiology Profile, stool LAB #: F000000-0000-0 PATIENT: Sample Patient ID: P12345 SEX: Female AGE: 37 CLIENT #: 12345 DOCTOR: Doctor's Data, Inc. 3755 Illinois Ave. St. Charles, IL 60174!Microbiology Profile, stool BACTERIOLOGY

More information

Effect of inulin on growth and antimicrobial activity of Lactobacillus spp.

Effect of inulin on growth and antimicrobial activity of Lactobacillus spp. Intl. J. Food. Ferment. 5(1): 47-52, June 2016 2016 New Delhi Publishers. All rights reserved DOI: 10.5958/2321-712X.2016.00006.5 Effect of inulin on growth and antimicrobial activity of Lactobacillus

More information

PRODUCTION OF PLAIN YOGHURT ADDING HAIRY BASIL MUCILAGE AS PREBIOTICS Piyanoot Noiduang, 1, * Areerat Ittakornpan 1, Vasinee Marukatat 1 1

PRODUCTION OF PLAIN YOGHURT ADDING HAIRY BASIL MUCILAGE AS PREBIOTICS Piyanoot Noiduang, 1, * Areerat Ittakornpan 1, Vasinee Marukatat 1 1 1 PRODUCTION OF PLAIN YOGHURT ADDING HAIRY BASIL MUCILAGE AS PREBIOTICS Piyanoot Noiduang, 1, * Areerat Ittakornpan 1, Vasinee Marukatat 1 1 Department of Food Technology, Faculty of Science, Siam University,

More information

Dialogue on the stability of probiotics

Dialogue on the stability of probiotics Dialogue on the stability of probiotics Learning from the US Dietary Supplements market Fernando Ferrer, MBA Auditorium ANVISA Basilia, Brazil. June 19, 2017 Disclaimer The views and opinions expressed

More information

WEBINAR Microbial Metabolism Associated with Health. 12 April 2018; CET

WEBINAR Microbial Metabolism Associated with Health. 12 April 2018; CET WEBINAR Microbial Metabolism Associated with Health 12 April 2018; 15.00-16.30 CET PROGRAMME Brief Introduction of Both Organisations and Scientific Background Dr Bettina Schelkle (ILSI Europe, BE) Dr

More information

UNDERSTANDING PROBIOTIC SUPPLEMENTS

UNDERSTANDING PROBIOTIC SUPPLEMENTS UNDERSTANDING PROBIOTIC SUPPLEMENTS Probiotics Health Canada Definition: Probiotics are considered to be live microorganisms which, when administered in adequate amounts, confer a health benefit on the

More information

UNDERSTANDING PROBIOTIC SUPPLEMENTS

UNDERSTANDING PROBIOTIC SUPPLEMENTS Slide 1 UNDERSTANDING PROBIOTIC SUPPLEMENTS Slide 2 Probiotics Health Canada Definition: Probiotics are considered to be live microorganisms which, when administered in adequate amounts, confer a health

More information

[Type text] [Type text] [Type text]

[Type text] [Type text] [Type text] [Type text] [Type text] [Type text] ISSN : 0974-7435 Volume 10 Issue 24 BioTechnology 2014 An Indian Journal FULL PAPER BTAIJ, 10(24), 2014 [16486-16491] Evaluation of Lactobacillus rhamnosus viability

More information

SCIENTIFIC OPINION. LACTORAL and maintenance of natural intestinal microflora during travel

SCIENTIFIC OPINION. LACTORAL and maintenance of natural intestinal microflora during travel The EFSA Journal (2008) 863, 1-8 SCIENTIFIC OPINION LACTORAL and maintenance of natural intestinal microflora during travel Scientific substantiation of a health claim related to LACTORAL (a combination

More information

PROBIOTICS NEGATIVE ASPECTS

PROBIOTICS NEGATIVE ASPECTS PROBIOTICS NEGATIVE ASPECTS Dr Ismail Moola Department of Medical Gastroenterology CMJAH University of Witwatersrand Putative Benefits of Probiotics Modulate Immune Intestinal Function Increase secretory

More information

Participants. probiotic. /placebo)

Participants. probiotic. /placebo) Table S1. Demographic characteristics of trials included in the meta-analysis. Study Birth weight (mean±sd) Gestation (wk) Feeding patterns Participants (probiotic /placebo) Probiotic agents Dosage Duration

More information

Index. B Bacitracin Lactobacillus acidophilus, 70 pyelonephritis, 246 Antifungals

Index. B Bacitracin Lactobacillus acidophilus, 70 pyelonephritis, 246 Antifungals A AAD, see Antibiotic-associated diarrhea Absorption Bifidobacterium species animals,67 humans,67 biotherapeutic agents, 47 Lactobacillus species, 61 Saccharomyces boulardii animals, 50, 51 humans,57 Absorption,

More information

PROFESSIONAL FORMULATION. ProBiotic & ProBiotic Multi. Probiotics: A Common Sense Discussion

PROFESSIONAL FORMULATION. ProBiotic & ProBiotic Multi. Probiotics: A Common Sense Discussion PROFESSIONAL FORMULATION ProBiotic & ProBiotic Multi ProBiotic (V-PB) 25 Billion C.F.U/gram 90 capsules per bottle Non-Dairy ProBiotic Multi (V-PBM) 15 Billion C.F.U./gram 45 capsules per bottle Non-Dairy

More information

IMPACT OF CONSUMPTION OF DIFFERENT LEVELS OF BIFIDOBACTERIUM LACTIS HN019 ON THE INTESTINAL MICROFLORA OF ELDERLY HUMAN SUBJECTS

IMPACT OF CONSUMPTION OF DIFFERENT LEVELS OF BIFIDOBACTERIUM LACTIS HN019 ON THE INTESTINAL MICROFLORA OF ELDERLY HUMAN SUBJECTS IMPACT OF B. LACTIS HN019 ON GUT FLORA OF THE ELDERLY IMPACT OF CONSUMPTION OF DIFFERENT LEVELS OF BIFIDOBACTERIUM LACTIS HN019 ON THE INTESTINAL MICROFLORA OF ELDERLY HUMAN SUBJECTS M. AHMED 2, J. PRASAD

More information

I S I F O O D P R OT E C T I O N. Microorganisms in food: THE GOOD, THE BAD & THE UGLY 192,720,000,000,000

I S I F O O D P R OT E C T I O N. Microorganisms in food: THE GOOD, THE BAD & THE UGLY 192,720,000,000,000 I S I F O O D P R OT E C T I O N Centre of Expertise for Applied Food Microbiology Microorganisms in food: 192,720,000,000,000 1 192,720,000,000,000 our daily intake 1,000 bacteria / portion Average American:

More information

Trend and Innovation of Pro and Prebiotics in Dairy Industry

Trend and Innovation of Pro and Prebiotics in Dairy Industry Emerging Dairy Ingredients: Innovation, Safety & Functionality Bogor, 9 June 2011 Trend and Innovation of Pro and Prebiotics in Dairy Industry Lilis Nuraida Southeast Asia Food and Agricultural Science

More information

EED INTERVENTIONS 3.0

EED INTERVENTIONS 3.0 EED INTERVENTIONS 3.0 Produced by: Kidane, L.; Osterman, A.; Babigumira, J. Agenda Agenda Project Background and Objectives Approach & Rationale Methods Results Discussion and Next Steps 2 Project Background

More information

Lorraine McIntyre Food Safety Specialist. Fermented Food Safety BC Food Protection Association November 7, 2016

Lorraine McIntyre Food Safety Specialist. Fermented Food Safety BC Food Protection Association November 7, 2016 Lorraine McIntyre Food Safety Specialist Fermented Food Safety BC Food Protection Association November 7, 2016 What is Fermentation? Fermentation is the chemical transformation of organic substances

More information

Probiotics: Their Role in Medicine Today. Objectives. Probiotics: What Are They? 11/3/2017

Probiotics: Their Role in Medicine Today. Objectives. Probiotics: What Are They? 11/3/2017 Probiotics: Their Role in Medicine Today Viki Barr Pharm.D., BCPS AQ ID Assistant Professor, Pharmacy Practice Rosalind Franklin University of Medicine and Science Clinical Pharmacist, Infectious Diseases

More information

Probiotic yoghurt and supplements: Retail sales in the EU Supplements and yoghurts to record 4%, and -1% growth over

Probiotic yoghurt and supplements: Retail sales in the EU Supplements and yoghurts to record 4%, and -1% growth over Probiotic yoghurt and supplements: Retail sales in the EU Supplements and yoghurts to record 4%, and -1% growth over 217-218 1 Probiotic yoghurt and supplements in the EU, retail sales, 29-218 7 1% 6 8%

More information

Safety Assessment of Yestimun Beta-Glucans

Safety Assessment of Yestimun Beta-Glucans Safety Assessment of Yestimun Beta-Glucans Name of Applicant: Leiber GmbH, Bramsche, Germany Contact person(s): Marion Hartong Novel Food Classification: Article 1.2(d) Introduction An application for

More information

Linking Research to Clinical Practice

Linking Research to Clinical Practice Probiotics and Oral Health Denise M. Bowen, RDH, MS Linking Research to Clinical Practice The purpose of Linking Research to Clinical Practice is to present evidence based information to clinical dental

More information

The role of intestinal microbiota in metabolic disease-a novel therapeutic target.

The role of intestinal microbiota in metabolic disease-a novel therapeutic target. Michael Connolly Department of Food Biosciences, The University of Reading The role of intestinal microbiota in metabolic disease-a novel therapeutic target. University of Reading 2008 www.reading.ac.uk

More information

Oral Health Applications for Probiotics

Oral Health Applications for Probiotics Oral Health Applications for Probiotics Andrew McBain Biofilm Research Group Manchester Pharmacy School University of Manchester Overview Oral microbiology introduction Dental probiotics and replacement

More information

Ther-Biotic Complete. Broad-spectrum synergistic multispecies probiotic formula. Supporting essential intestinal microdiversity

Ther-Biotic Complete. Broad-spectrum synergistic multispecies probiotic formula. Supporting essential intestinal microdiversity Ther-Biotic Complete Broad-spectrum synergistic multispecies probiotic formula Supporting essential intestinal microdiversity Ther-Biotic Complete Broad-spectrum synergistic multispecies probiotic formula

More information

We are IntechOpen, the first native scientific publisher of Open Access books. International authors and editors. Our authors are among the TOP 1%

We are IntechOpen, the first native scientific publisher of Open Access books. International authors and editors. Our authors are among the TOP 1% We are IntechOpen, the first native scientific publisher of Open Access books 3,350 108,000 1.7 M Open access books available International authors and editors Downloads Our authors are among the 151 Countries

More information

continuing education for pharmacists Volume XXXIV, No. 8

continuing education for pharmacists Volume XXXIV, No. 8 continuing education for pharmacists Volume XXXIV, No. 8 Prebiotics, Probiotics, and Synbiotics: Their Therapeutic Role Cortney M. Mospan, PharmD, BCACP, CGP, Assistant Professor of Pharmacy, Wingate University

More information

ANTIBIOTIC ALTERNATIVES FOR YOUNGSTOCK

ANTIBIOTIC ALTERNATIVES FOR YOUNGSTOCK ANTIBIOTIC ALTERNATIVES FOR YOUNGSTOCK Jud Heinrichs Dairy Science Penn State Topics for today Overview of the issue Product types Some data How to pick a product How management helps 1 Overview The emergence

More information

Emerging Probiotic Research: A Guide to the Use of Probiotics in Clinical Practice

Emerging Probiotic Research: A Guide to the Use of Probiotics in Clinical Practice Emerging Probiotic Research: A Guide to the Use of Probiotics in Clinical Practice Donald Brown, ND April 13, 2010 Probiotics WHO Definition: Live microorganisms, which when administered in adequate amounts,

More information

Administration of Bifidobacterium to Infants with Atopic Dermatitis: Changes in Fecal Microflora and Clinical Symptoms

Administration of Bifidobacterium to Infants with Atopic Dermatitis: Changes in Fecal Microflora and Clinical Symptoms Administration of Bifidobacterium to Infants with Atopic Dermatitis: Changes in Fecal Microflora and Clinical Symptoms Shoichiro Taniuchi, MD * Kazuhiro Hattori, MD * Akemi Yamamoto, MD * Misa Sasai, MD

More information

Changes in the Microflora of Bovine Colostrum During Natural Fermentation

Changes in the Microflora of Bovine Colostrum During Natural Fermentation 27 f. Milk Food Techno/. Vol. 39. No. I, Pages 27-31!January, 1976) Copyright 1976, International Association of Milk, Food, and Environmental Sanitarians Changes in the Microflora of Bovine Colostrum

More information

the (st)art of growing

the (st)art of growing the (st)art of growing The art of rearing Emulsizym 4seasons Nukamel s range of whey-based milk replacers (CMR) is recommended for the stable and efficient rearing of dairy calves. The acidified milk replacers

More information

Probiotics: Implications for Pediatric Health

Probiotics: Implications for Pediatric Health Probiotics: Implications for Pediatric Health PREFACE It is no longer questioned that the interplay between our genes, nutrition, and our environment hold the keys to growth, development, and health.

More information

CHAPTER 8 IN VITRO CHARACTERIZATION OF LACTIC ACID BACTERIA STRAINS FOR PROBIOTIC CHARACTERISTICS

CHAPTER 8 IN VITRO CHARACTERIZATION OF LACTIC ACID BACTERIA STRAINS FOR PROBIOTIC CHARACTERISTICS CHAPTER 8 IN VITRO CHARACTERIZATION OF LACTIC ACID BACTERIA STRAINS FOR PROBIOTIC CHARACTERISTICS 8.1. Introduction Probiotic bacteria must overcome physical and chemical barriers such as acid and bile

More information