SwissProt/ TrEmbl Acc. No. 1 Description. Found in Other Studies 5. MW (kda) 2 pi 3 Function 4

Transcription

1 P02774 Vitamin D-binding protein precursor Cell communication c, a,b S,C P08833 Insulin-like growth factor binding protein 1 precursor Cell communication c, a P02760 AMBP protein precursor [Contains: Alpha-1-microglo Cell communication c, a,b S P02751 Fibronectin precursor Cell communication d S P00450 Ceruloplasmin precursor Cell communication c S,C P02763 Alpha-1-acid glycoprotein 1 precursor Cell communication a S,C P02452 Collagen alpha 1 (I) chain precursor Cell communication d S P02790 Hemopexin precursor Defense response c S,C P04217 Alpha-1B-glycoprotein precursor Defense response c, a S,C P01042 Kininogen precursor Enzyme activity c S,C P02749 Beta-2-glycoprotein I precursor Cell communication S,C P61769 Beta-2-microglobulin precursor Signal transduction c, a P02753 Plasma retinol-binding protein precursor Cell communication S P09486 SPARC precursor Development d S P19652 Alpha-1-acid glycoprotein 2 precursor Cell communication S P00747 Plasminogen precursor Enzyme activity S,C P02771 Alpha-fetoprotein precursor Cell communication b S P01236 Prolactin precursor Signal transduction P02461 Collagen alpha 1 (III) chain precursor Cell communication P02647 Apolipoprotein A-I precursor Cell communication d S,C P80370 Delta-like protein precursor Development P25311 Zinc-alpha-2-glycoprotein precursor Cell proliferation P20908 Collagen alpha 1 (V) chain precursor Cell communication P02765 Alpha-2-HS-glycoprotein precursor Metabolism a,d S,C P13727 Eosinophil granule major basic protein precursor Defense response P04196 Histidine-rich glycoprotein precursor Enzyme activity c S Q16610 Extracellular matrix protein 1 precursor Signal transduction S,C P01033 Metalloproteinase inhibitor 1 precursor Metabolism S P43652 Afamin precursor Cell communication S P08603 Complement factor H precursor Defense response c S,C P12111 Collagen alpha 3 (VI) chain precursor Cell communication c P41222 Prostaglandin-H2 D-isomerase precursor Cell communication S P23142 Fibulin-1 precursor Cell communication c S P17936 Insulin-like growth factor binding protein 3 precursor Development

2 P01034 Cystatin C precursor Enzyme activity P18065 Insulin-like growth factor binding protein 2 precursor Cell communication S Q16270 Insulin-like growth factor binding protein 7 precursor Cell communication d Q12841 Follistatin-related protein 1 precursor Cell proliferation c P01008 Antithrombin-III precursor Enzyme Activity c S,C P98160 Basement membrane-specific heparan sulfate proteo Cell communication c P10451 Osteopontin precursor Cell communication c P09466 Glycodelin precursor Development P12109 Collagen alpha 1 (VI) chain precursor Cell communication d P51884 Lumican precursor Cell communication c S Q14766 Latent transforming growth factor beta binding protein Enzyme activity P22692 Insulin-like growth factor binding protein 4 precursor Metabolism P62328 Thymosin beta Metabolism C Q12805 EGF-containing fibulin-like extracellular matrix protein Defense response S P10909 Clusterin precursor Signal transduction c S P04004 Vitronectin precursor Cell communication c S,C Q15582 Transforming growth factor-beta induced protein IG-H Cell proliferation d S P61626 Lysozyme C precursor Enzyme activity C P00751 Complement factor B precursor Metabolism c, a S P13987 CD59 glycoprotein precursor Defense response c C P35555 Fibrillin-1 precursor Development P08294 Extracellular superoxide dismutase [Cu-Zn] precurso Metabolism Q9HC84 Mucin-5B precursor Cell communication C P07998 Ribonuclease pancreatic precursor Enzyme activity P02788 Lactotransferrin precursor Defense response a S,C Q9UBC9 Small proline-rich protein FNA C P01344 Insulin-like growth factor II precursor Signal transduction Q14118 Dystroglycan precursor Signal transduction P00748 Coagulation factor XII precursor Enzyme activity S P07602 Proactivator polypeptide precursor [Contains: Saposi Metabolism P01019 Angiotensinogen precursor Metabolism c S P01833 Polymeric-immunoglobulin receptor precursor Cell communication c C P02766 Transthyretin precursor Cell communication c, a S P02768 Serum albumin precursor Cell communication b,d S,C

3 P05156 Complement factor I precursor Defense response S Q15113 Procollagen C-proteinase enhancer protein precurso Development S P11464 Pregnancy-specific beta-1-glycoprotein 1 precursor FNA c P29279 Connective tissue growth factor precursor Metabolism Q9UGM5 Fetuin-B precursor Metabolism P02671 Fibrinogen alpha/alpha-e chain precursor Cell proliferation S,C P00746 Complement factor D precursor Defense response S P02748 Complement component C9 precursor Defense response c S P07476 Involucrin Development C P05090 Apolipoprotein D precursor Metabolism c S P01243 Chorionic somatomammotropin hormone precursor Signal transduction a P08123 Collagen alpha 2(I) chain precursor Cell communication d P05362 Intercellular adhesion molecule-1 precursor Cell communication P24593 Insulin-like growth factor binding protein 5 precursor Cell communication P02679 Fibrinogen gamma chain precursor Cell proliferation c S,C Q07654 Trefoil factor 3 precursor Defense response P35321 Cornifin A Development C O95633 Follistatin-related protein 3 precursor FNA P31949 Calgizzarin Metabolism P61916 Epididymal secretory protein E1 precursor Metabolism O43278 Kunitz-type protease inhibitor 1 precursor Enzyme activity P04278 Sex hormone-binding globulin precursor Cell communication S P16070 CD44 antigen precursor Cell communication Q13421 Mesothelin precursor Cell communication c P01028 Complement C4 precursor Defense response c S,C P08174 Complement decay-accelerating factor precursor Defense response P31025 Von Ebner's gland protein precursor Defense response c, a Q10588 ADP-ribosyl cyclase 2 precursor Defense response S P03973 Antileukoproteinase 1 precursor Enzyme activity C P04080 Cystatin B Enzyme activity a C P02652 Apolipoprotein A-II precursor Metabolism S P10599 Thioredoxin Metabolism C Q96IY4 Carboxypeptidase B2 precursor Metabolism S P11684 Uteroglobin precursor Signal transduction

4 P55290 Cadherin-13 precursor Cell organization P22105 Tenascin-X precursor Cell communication S P80188 Neutrophil gelatinase-associated lipocalin precursor Cell communication S,C P01024 Complement C3 precursor Defense response c S,C P01860 Ig gamma-3 chain C region Defense response S P48745 NOV protein homolog precursor Development P03952 Plasma kallikrein precursor Enzyme activity S P17900 Ganglioside GM2 activator precursor Enzyme activity P04156 Major prion protein precursor Metabolism P07988 Pulmonary surfactant-associated protein B precursor Metabolism c P01215 Glycoprotein hormones alpha chain precursor Signal transduction Q14767 Latent transforming growth factor-beta-binding protei Signal transduction P00734 Prothrombin precursor Enzyme activity c S P02787 Serotransferrin precursor Defense response a,c,d S,C P09228 Cystatin SA precursor Enzyme activity Q03591 Complement factor H-related protein 1 precursor Cell communication S P12830 Epithelial-cadherin Cell communication P14543 Nidogen Cell communication P16949 Stathmin Cell communication P49747 Cartilage oligomeric matrix protein Cell communication S P protein epsilon Cell communication d P protein zeta/delta Cell communication d C P68871 Hemoglobin subunit beta Cell communication a S Q13908 Alpha-2 type V collagen Cell communication Q14036 Alpha-2 type I collagen Cell communication Q14112 Nidogen Cell communication Q5XKQ4 Alpha-2-glycoprotein 1, zinc Cell communication S Q6GRB6 Fetuin-B Cell communication S Q6GTW4 Cadherin 13, preproprotein Cell communication Insulin-like growth factor binding protein, acid labile Q8TAY0 subunit Cell communication S Q92496 Complement factor H-related protein Cell communication P35609 Alpha-actinin Cell organization P53485 Actin, cytoplasmic Cell organization

5 P68133 Actin, alpha skeletal muscle Cell organization S P02675 Fibrinogen beta chain Cell proliferation d, a S,C P60709 Actin, cytoplasmic Cell proliferation S,C P protein gamma Cell proliferation d P01011 Alpha-1-antichymotrypsin Defense response S,C P01595 Ig kappa chain V-I region Bi Defense response P01598 Ig kappa chain V-I region EU Defense response S P01603 Ig kappa chain V-I region Ka Defense response P01605 Ig kappa chain V-I region Lay Defense response P01621 Ig kappa chain V-III region NG Defense response S P01622 Ig kappa chain V-III region Ti Defense response S P01624 Ig kappa chain V-III region POM Defense response S P01625 Ig kappa chain V-IV region Len Defense response S P01766 Ig heavy chain V-III region BRO Defense response P01767 Ig heavy chain V-III region BUT Defense response P01857 Ig gamma-1 chain C region Defense response S,C P01859 Ig gamma-2 chain C region Defense response S,C P01861 Ig gamma-4 chain C region Defense response S,C P01876 Ig alpha-1 chain C region Defense response S,C P01877 Ig alpha-2 chain C region Defense response S,C P04083 Annexin A Defense response d S,C P04206 Ig kappa chain V-III region GOL Defense response S P04434 Ig kappa chain V-III region VH Defense response S P05109 Protein S100-A Defense response S,C P05155 Plasma protease C1 inhibitor Defense response c S P05164 Myeloperoxidase* Defense response C P06681 Complement C Defense response S P06702 Protein S100-A Defense response S,C P08571 Monocyte differentiation antigen CD Defense response S P08697 Alpha-2-antiplasmin Defense response c S P10643 Complement component C Defense response S P15814 Immunoglobulin lambda-like polypeptide Defense response P18135 Ig kappa chain V-III region HAH Defense response S P20160 Azurocidin* Defense response

6 P40121 Macrophage capping protein Defense response d P49913 Antibacterial protein FALL-39* Defense response Long palate, lung and nasal epithelium carcinomaassociated protein 1* Defense response C Q8TDL5 Q9UGM3 Glycoprotein 340* Defense response P05452 Tetranectin Development S P06741 Myosin light chain 3, skeletal muscle isoform Development P07355 Annexin A Development d S,C P07996 Thrombospondin Development S P36955 Pigment epithelium-derived factor Development c S Q9UBI9 Headcase protein homolog Development P30740 Leukocyte elastase inhibitor* Enzyme activity C P05543 Thyroxine-binding globulin Enzyme activity c S P05546 Heparin cofactor Enzyme activity S P08185 Corticosteroid-binding globulin Enzyme activity c S P09525 Annexin A Enzyme activity d Q02325 Plasminogen-related protein B Enzyme activity S P02750 Leucine-rich alpha-2-glycoprotein FNA S P13796 Plastin-2* FNA S,C Q2XP30 Mutant beta-globin FNA Q5SYV9 Lipocalin FNA Q6EMK4 Vasorin FNA Q92520 Protein FAM3C FNA Q9UJ14 Gamma-glutamyltransferase FNA Q9Y5Z4 Heme-binding protein FNA O00187 Mannan-binding lectin serine protease Metabolism S P00918 Carbonic anhydrase Metabolism P01023 Alpha-2-macroglobulin Metabolism S P06727 Apolipoprotein A-IV Metabolism c,d S P08709 Coagulation factor VII Metabolism P15144 Aminopeptidase N Metabolism S P18669 Phosphoglycerate mutase Metabolism d C P19440 Gamma-glutamyltranspeptidase Metabolism P22352 Glutathione peroxidase Metabolism

7 P36222 Chitinase-3-like protein Metabolism P43251 Biotinidase Metabolism S P55058 Phospholipid transfer protein Metabolism S P60174 Triosephosphate isomerase Metabolism d C P S ribosomal protein S27a Metabolism P S ribosomal protein L Metabolism P62988 Ubiquitin Metabolism C Q16819 Meprin A subunit alpha Metabolism S Q53XZ5 Tyrosine 3-monooxygenase/tryptophan 5- monooxygenase Metabolism Q5T986 Inter-alpha (Globulin) inhibitor, H2 polypeptide Metabolism CDNA FLJ32377 fis, clone SKMUS , highly similar to Polyubiquitin Metabolism Q96MH4 Q96PQ8 Factor VII active site mutant immunoconjugate Metabolism Q14406 Chorionic somatomammotropin hormone-like Signal transduction Q53YY1 Angiotensinogen Signal transduction S Q7Z7P3 Coagulation factor II, Signal transduction Q86VW7 Colony stimulating factor 1 receptor, Signal transduction Q8WZA4 Collectin placenta Signal transduction Proteins that were identified in experiment are listed with their SwissProt or TrEMBL accession 1. Molecular weight (kda) 2 and pi 3 calculated using CalPI/ tool available on SwissProt website. 4 Functional annotation is performed using procedure outlined in methods section. FNA-Function not assigned. 5 AF proteins that were also found in other amniotic fluid (a I,b II,c III ) and amnion cell (d IV ) studies are marked accordingly. 6 AF proteins that are also present in Serum (S) and Cervico-vagianl Fluid (C) are also marked (see results section for details). * Proteins identified in amniotic fluid with infection. I - Park, SJ et. al. Proteomics, 2006, 6, (1), ; II - Nilsson S et. al. J. Proteome Research, 2004, 3, (4), ; III - Michel, P. E. et. al. Electrophoresis, 2006, 7, 7.; IV- Tsangaris, G. et. al. Electrophoresis, 2005, 26, (6), ; Proteins are also color coded for their role in fetal biology (see results for details): Red-Complement and Coagulation cascades, Grey-Metabolism, Green - Defense response, Yellow-Signal transduction, Orange-Development, and Cyan- Transport