WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES. World Journal of Pharmacy and Pharmaceutical Sciences SJIF Impact Factor 6.
|
|
- Alisha Shields
- 6 years ago
- Views:
Transcription
1 WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES Neeru et al. SJIF Impact Factor Volume 5, Issue 6, Research Article ISSN DETERMINATION OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT AND TOTAL ANTIOXIDANT CAPACITY OF DIFFERENT EXTRACTS OF ROYLEA ELEGANS WALL (AERIAL PARTS) Neeru 1*, Shilpi Kashyap 1, Esha Vatsa 2, Jitendra Singh 3 and Ankush Sundriyal 4 1 Himachal Institute of Pharmacy, Paonta Sahib District Sirmour. 2 Manav Bharti University, Solan, Himachal Pradesh. 3,4 SBSPGI, Balawala Dehradun. Article Received on 13 April 2016, Revised on 01 May 2016, Accepted on 22 May 2016 DOI: /wjpps *Corresponding Author Neeru Himachal Institute of Pharmacy, Paonta Sahib District Sirmour. ABSTRACT Petroleum ether, chloroform, ethyl acetate, methanol and water s of Roylea elegans (aerial parts) was screened for assessing bioactive phytochemical constituents. The preliminary phytochemical screening using the standard phytochemical tests detected the presence of alkaloids, steroids, flavonoids, terpenoids and phenols in different solvent s. Total phenolic and flavonoid contents along with total antioxidant capacity of the ethyl acetate of Roylea elegans (aerial parts) was evaluated to explore the reliable and potential sources of novel natural antioxidants. The ethyl acetate showed high flavonoid content. Due to high flavonoid content it showed better antioxidant activity. KEYWORDS: Roylea elegans, Total phenolic content, total flavonoid content, antioxidant activity. INTRODUCTION In nature plants gives more products which are useful for human beings. These natural products are used for several diseases and also used as antioxidant. Plants give secondary metabolites which are used for several diseases. These secondary metabolites are alkaloids, glycosides, terpenoids, flavonoids, phenolic compound and tannins. These secondary metabolites are produced from various parts of plants like stem, leaves, flowers, fruits and Vol 5, Issue 6,
2 roots. These secondary metabolites are biologically active and show better activity. Flavonoid and phenolic content shows better antioxidant activity. Roylea elegans a plant belonging to family lamiaceae shows better antioxidant activity due to presence of good flavonoid content. Roylea elegans is a shrub of monotypic genus. The family lamiaceae contain 22 species. Plant Roylea elegans belonging to family lamiaceae. Leaves part of this plant (decoction) is traditionally used as a bitter tonic and also as a febrifuge. It is also used as a tonic in contusions. Leaves are used in skin disease and fever. The leaves of Roylea elegans contains various phytoconstituents like betulin, beta-sitosterol, beta-amyrin, glucose, sti gmasterol, cetyl alcohol, and palmitic, stearic, oleic, gallic, oxalic, fructose, arabinose and tartaric acids. MATERIALS AND METHODS chemicals and reagents All the chemicals which were used in study are of analytical grade and purchased from the Himedia Lab. Pvt. Ltd, Rankem. Plant selection and identification The plant material (Roylea elegans) investigated in the present study was collected from Nauni, near Solan, Himachal Pradesh. The plant material was identified and authenticated at the Herbarium of Council of Scientific and Industrial Research - National Institute of Science Communication and Information Resources (CSIR-NISCAIR), Delhi vide reference no. NISCAIR/RHMD/Consult/2014/2781/160. Preparation of plant The dried aerials parts of plant were collected. Then aerial parts of the plant were pulverized through a mechanical grinder. Powder material was dried in hot air oven at moderate temperature. The Powdered material was subjected to successive Soxhlet ion by solvents in increasing order of polarity viz. Petroleum ether, chloroform, ethyl acetate then macerate with water for 24hour. The was concentrated and stored at 4 0 c until further use in the experiment (Chatwal 2011). Phytochemical evaluation of the Vol 5, Issue 6,
3 The preliminary phytochemical screening of the aerial parts mainly done for the evaluation of the various phytoconstituents such as steroids, tannin, Alkaloids, Flavonoids and glycosides were present in the aerial parts of different s of R. elegans. Determination of total phenolics content The Total Phenolic content of of R. elegans was estimated according to the method described in Ayurvedic Pharmacopoeia of India. The total phenol content was expressed in milligrams of gallic acid equivalents per gram of. Prepare a stock solution (1mg/ml) of the in methanol. From the stock solution, take suitable quantity of the into 25ml volumetric flask and add 10 ml of water and 1.5ml of folin ciocalteau reagent. Keep the mixture for 5min and then add 4ml of 20% sodium carbonate solution and make up to 25ml with double distilled water Keep the mixture for 30min and record absorbance at 765nm. Calculate percentage of total phenolics from calibration curve of gallic acid prepared by using the above procedure and express total phenolics as percentage of gallic acid. [5] Total phenolic content was calculated with the help of standard curve equation and the formula was below Total phenolic content (%w/w)= GAE V D W Where, GAE Gallic acid equivalent (µg/ml) V Total volume of sample (ml) D Dilution factor W Sample weight (gm) Determination of total flavonoid content The total flavonoid content of of R. elegans was estimated according to the aluminium chloride method as follows: The aluminium chloride method was used for the determination of the total flavonoid content of the sample s. Rutin was used to make the calibration curve. Ten milligrams of rutin was dissolved in 80% ethanol and then diluted to 25, 50 and 100 μg/ml. The standard solutions (0.5 ml) were prepared by mixing 1.5 ml of 95% ethanol, 0.1 ml of 10% aluminum chloride, 0.1 ml of 1M potassium acetate and 2.8 ml of distilled water. The absorbance of the reaction mixture was measured after 30 min at 415 nm. The amount of 10% aluminum chloride was substituted by the same amount of distilled water in blank. [11] Vol 5, Issue 6,
4 Total flavonoid content was calculated with standard curve equation and the formula Total flavonoid content (% w/w)= RE V D W Where, RE Rutin equivalent (µg/ml) V Total volume of sample (ml) D Dilution factor W Weight of sample (gm) Antioxidant activity DPPH DPPH will be assayed as described by Elizabeth and Rao. The reaction mixture contained 1.0 ml of 0.3mM DPPH in 50 ml of methanol will be added with concentrations ranging from μg/ml.the mixture of DPPH in methanol used as positive control and methanol alone served as blank. When DPPH reacts with antioxidants in the sample, it was reduced and the colour changed from deep violet to light yellow and measured at 517 nm. Quercetin will be used as a reference standard. Scavenging activity (%) = [A517 (control) A517 (sample)/ A517 (control)] X 100. [4] RESULT AND DISCUSSION Table 1: Phytochemical screening of different s of Roylea elegans Wall. (aerial parts) S. no. Phytochemical tests Pet. Ether Chloroform Ethyl acetate Methanol Water 1. Carbohydrate Protein Amino acid Fats Steroid Triterpenoides Glycosides Flavonoids Alkaloids Tannins Total Phenolic Content Table 2: Standard curve of gallic acid S. no. Concentration(µg/ml) Absorbance Vol 5, Issue 6,
5 Figure 1: Standard curve of gallic acid Table 3: Total phenolic content in different s S. no. Extracts Total phenolic content (%w/w) 1. Petroleum ether Chloroform Ethyl acetate Methanol Water Figure 2: Total phenolic content Total Flavonoid Content Table 4: Standard curve of rutin Vol 5, Issue 6,
6 S. no. Concentration (µg/ml) Absorbance Figure 3: Standard curve of Rutin Table 5: Total flavonoid content in different s. S. no. Extracts Total flavonoid content (%w/w) 1. Petroleum ether Chloroform Ethyl acetate Methanol Water Figure 4: Total flavonoid content Total Antioxidant Capacity Table 6: DPPH scavenging activity Vol 5, Issue 6,
7 % Inhibition S. Concentration Petroleum Ethyl no. (µg/ml) Rutin Chloroform Methanol Water ether acetate Figure 5: DPPH scavenging activity CONCLUSION In our present study total phenolic content of in different s were showing that water has high phenolic content and total flavonoid content of different showing ethyl acetate has high. Total antioxidant activity of different e s was showing that ethyl acetate has high antioxidant power due to high flavonoid content. REFERENCES 1. Benzie, I. F. F. & Strain, J. J.,The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power : The FRAP assay, Analytical Biochemistry, 1996; Bhandari, S. L. Bisht, S. S. Bisht, N. S. (Department of Botany, H.N.Bal University, Campus Pauri,(Garhwal), Micro propagation of Roylea elegans Wall. an important medicinal herb, Journal of Medicinal and Aromatic Plant Sciences v, 2008; 30(4): Dobhal, M. P. & Joshi B. C., Chemical investigations of Roylea elegans Wall. Part I, Herba Pol., 1979; Elizabeth, K. & Rao, M. N. A., Oxygen radical scavenging activity of curcumin. Int J Pharmaceut, 1990; Vol 5, Issue 6,
8 5. Indian Pharmacopoeia. I.P, Vol I. Ministry of Health and Family Welfare, Government of India, Published by the Indian Pharmacopoeia Commission, Ghaziabad, India, 2010; 82-83: 139 & Khandelwal, K.R. Practical pharmacognosy, 19 th edition, 2008; 9: , Ranganayaki S. Gusain P. S. Singh A. K. The chemical constituents of leaves of Roylea elegans. J Sci Res Plants & Medicines, 1985; Sai Khan, M. S. Howard, L. R. Miller, J. C. antioxidant activity and total phenolics in Solanum, J Food Sci, 1995; 60(2): Upadhyay, G. Kamboj, P. Malik, J. International Journal of Research in Pharmaceutical and Biomedical Sciences, 2011; 2(4). 10. Upadhyay, G. Maurya, H. Chauhan, N. Traditional claim of Roylea elegans Wall. On liver disorder., Woisky, R. and Salatino, A. Analysis of propolis: some parameters and procedures for chemical quality control. J. Apic. Res., 1998; 37: Vol 5, Issue 6,
Research Article GALLIC ACID AND FLAVONOID ACTIVITIES OF AMARANTHUS GANGETICUS
ISSN 2395-3411 Available online at www.ijpacr.com 238 Research Article GALLIC ACID AND FLAVONOID ACTIVITIES OF AMARANTHUS GANGETICUS G. Jyoti Jain 1* and S. Ramachandra Setty 2 1 Department of Pharmacology,
More informationEstimation of total phenolic and flavonoid content of Hibiscus furcatus Roxb leaves
IJPAR Vol.6 Issue 1 Jan - Mar -217 Journal Home page: ISSN:232-2831 Research article Open Access Estimation of total phenolic and flavonoid content of Hibiscus furcatus Roxb leaves Najuma Salim*, Rahul
More informationEvaluation of Diuretic Activity of Jussiaea Suffruticosa Linn.
Evaluation of Diuretic Activity of Jussiaea Suffruticosa Linn. R.Mythreyi 1, N.Rajkumar 1* and E.Sasikala 2 1 Maharaji College of Pharmacy, Besant Nagar, Chennai-90. 2 Central Research Institute for Siddha,
More informationPhytochemical Analysis and Antioxidant property of Aegle marmelos Extracts
ISSN: 2319-7706 Volume 4 Number 9 (2015) pp. 826-830 http://www.ijcmas.com Original Research Article Phytochemical Analysis and Antioxidant property of Aegle marmelos Extracts Anjay Kumar Gupta*, Sumeet
More informationIn Vitro Antioxidant Activity and Phytochemical Screening of Pholidota articulata
Human Journals Research Article November 2016 Vol.:5, Issue:1 All rights are reserved by Darshan Singh et al. In Vitro Antioxidant Activity and Phytochemical Screening of Pholidota articulata Keywords:
More informationPRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO FREE RADICAL SCAVENGING ACTIVITY OF MELOCHIA CORCHORIFOLIA PLANT EXTRACTS
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article PRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO FREE RADICAL SCAVENGING ACTIVITY OF MELOCHIA
More informationInternational Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: Vol.9, No.4, pp , 2016
International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: 0974-4304 Vol.9, No.4, pp 215-219, 2016 Phytochemical Analysis and Total Phenolic and Flavonoid Contents Determination of Methanolic
More informationDetermination of total phenolic, flavonoid content and free radical scavenging activities of common herbs and spices.
2014; 3 (3): 104-108 E: ISSN 2278-4136 P: ISSN 2349-8234 JPP 2014; 3 (3): 104-108 Received: 07-08-2014 Accepted: 08-09-2014 Ovais Ullah Shirazi. Muhammad Muzaffar Ali Khan Khattak. (a) (b)non Communicable
More informationAntioxidant Activity of the plant Andrographis paniculata (Invitro)
Chapter 4 Antioxidant Activity of the plant Andrographis paniculata (Invitro) 4.1 INTRODUCTION Antioxidants prevents or repairs the cells against reactive oxygen species, reduces damage caused by free
More informationPRELIMINARY PHYTOCHEMICAL STUDIES ON SOLANUM SURATTENSE BURM.F. SEEDS
Ancient Science of Life Vol : XXVI (1&2) July, August, September, October, November, December 2006 PRELIMINARY PHYTOCHEMICAL STUDIES ON SOLANUM SURATTENSE BURM.F. SEEDS S. SHAHILADEVI, G. JAYANTHI AND
More informationInvestigation of phytochemicals & estimation of total phenolic and flavonoid content of myxopyrum smilacifolium (wall.
IJPAR Vol.8 Issue 1 Jan Mar - 2019 Journal Home page: ISSN:2320-2831 Research article Open Access Investigation of phytochemicals & estimation of total phenolic and flavonoid content of myxopyrum smilacifolium
More informationIn vitro antioxidant activity of a flavonoid compound isolated from methanolic extract of Helianthus annuus leaves (Asteraceae)
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2017, 9 [6]:28-34 [http://scholarsresearchlibrary.com/archive.html] ISSN 0975-5071 USA CODEN: DPLEB4
More informationAntioxidant Activity by DPPH Radical Scavenging Method of Ageratum conyzoides Linn. Leaves
American Journal of Ethnomedicine, 2014, Vol. 1, No. 4, 244-249 Available online at http://www.ajethno.com American Journal of Ethnomedicine Antioxidant Activity by DPPH Radical Scavenging Method of Ageratum
More informationTLC, HPTLC and Quantitative Estimation of Acetone, Methanolic and Hydro-Alcoholic Extract of Stem Bark of Bauhinia purpurea Linn.
Available online on www.ijppr.com International Journal of Pharmacognosy and Phytochemical Research 215; 7(3); 49-415 Research Article ISSN: 975-4873 TLC, HPTLC and Quantitative Estimation of Acetone,
More informationPhytochemical composition and in vitro antioxidant activity of methanolic and aqueous extracts of aerial part of Pentatropis nivalis (Asclepiadaceae)
Babre et al. DOI: 10.5138/09750185.2219 International Journal of Phytomedicine 2018;10(1):68-72 Original Research Article Phytochemical composition and in vitro antioxidant activity of methanolic and aqueous
More informationStudies on the Antioxidant Properties of Various extracts of Hippophae rhamnoide
International Journal of Environment, Agriculture and Biotechnology (IJEAB) Vol-2, Issue-3, May-Jun- 217 http://dx.doi.org/.22161/ijeab/2.3.14 ISSN: 2456-1878 Studies on the Antioxidant Properties of Various
More information3. PRELIMINARY PHYTOCHEMICAL SCREENING
93 3. PRELIMINARY PHYTOCHEMICAL SCREENING 3.1 INTRODUCTION All the drugs- Ayurvedic, Unani and Herbal extracts were subjected to preliminary phytochemical screening to test the presence of alkaloids, carbohydrates
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research J. Chem. Pharm. Res., 2010, 2(2): 107-111 ISSN No: 0975-7384 Phytochemical Screening of Curculigo Orchioides Gaertn. Root
More informationPharmacognostic Study Of Plant Euphorbia hirta L
Pharmacognostic Study Of Plant Euphorbia hirta L Research Article Giram Ganesh Bhagwat 1 *,Chaulang Ganesh Mahadev 1,Ghodke Dhananjay Sahebrao 1, Patil Kundan Suresh 1,Yeole Pramod Govindrao 1,Ganjiwale
More informationDetermination of Tanninoids. Analytical Pharmacognosy
QUIZ If the manager of a phytopharmaceutical industry wish to buy one chromatographic equipment, which one you recommend, HPLC or TLC densitometer. What are the reasons to support your recommendation.
More informationINTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES
INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES Development of standardization of trikatu churna: A spectrophotometric approach Manjesh Thakur*, P. Porwal, P. Pal, K. Shukla and S.C. Mahajan Mahakal
More informationEVALUATION OF INVITRO ANTIOXIDANT ACTIVITY OF ETHANOLIC ROOT EXTRACT OF CURCULIGO ORCHIOIDES
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article EVALUATION OF INVITRO ANTIOXIDANT ACTIVITY OF ETHANOLIC ROOT EXTRACT OF CURCULIGO ORCHIOIDES
More informationScholars Research Library. Determination of Phenol and flavonoid content from Vateria indica (Linn)
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2012, 4 (1):222-226 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationPhytochemical Investigation and Invitro Evaluation of Antioxidant Activity of Mucuna Pruriens Root
Phytochemical Investigation and Invitro Evaluation of Antioxidant Activity of Mucuna Pruriens Root OKPOSO 1**, A. Michael., ETINAGBEDIA 2, A., OROGU 3, O. Joshua, OKUDA 4, A. Frank 1** Department of Chemistry,
More informationPharmacognostic and preliminary phytochemical analysis of Aegle marmelos L. and Centella asiatica L.
Pharmacognostic and preliminary phytochemical analysis of Aegle marmelos L. and Centella asiatica L. A. Vijayalakshmi, G. Sakthivigneswari and Suganya, J. Department of Botany, Avinashilingam Institute
More informationPhytochemical and Physico-Chemical Analysis of Siddha Preparation Magizham Pattai Chooranam
International Journal of Research in Pharmacy and Biosciences Volume 1, Issue 2, December 2014, PP 11-15 ISSN 2394-5885 (Print) & ISSN 2394-5893 (Online) Phytochemical and Physico-Chemical Analysis of
More information6 CHAPTER-6 TOTAL PHENOLIC AND FLAVONOID CONTENT DETERMINATION
6 CHAPTER-6 TOTAL PHENOLIC AND FLAVONOID CONTENT DETERMINATION 6.1 PHENOLIC COMPOUNDS Phenolic compounds are a group of chemical compounds that are widely distributed in nature. They are simple compounds
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(2):465-471 Evolution of Free Radical Scavenging Potential
More informationInternational Journal of Drug Research and Technology
Int. J. Drug Res. Tech. 2012, Vol. 2 (2), 203-207 ISSN 2277-1506 International Journal of Drug Research and Technology Available online at http://www.ijdrt.com/ Original Research Paper COMPARATIVE PHYTOCHEMICAL
More informationInternational Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: Vol.9, No.4, pp , 2016
International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: 0974-4304 Vol.9, No.4, pp 261-265, 2016 A Research Article on Phytochemical and Physicochemical Evaluation of Leaves & Stems of Kalanchoe
More informationComparative Physicochemical and Phyto-Chemical Screening of Glucomap: An Herbal Antidiabetic Tablet
Comparative Physicochemical and Phyto-Chemical Screening of Glucomap: An Herbal Antidiabetic Tablet Satyaendra K. Shrivastava* 1, P.K. Dubey 1, B. Shrivastava 2, Pankaj Sharma 2 * 1 Swami Vivekanand College
More informationJournal of Pharmacognosy and Phytochemistry. Preliminary Phytochemical Screening and HPTLC Fingerprinting of Leaf Extracts of Pisonea aculeata
ISSN 2278-4136 ZDB-Number: 2668735-5 IC Journal No: 8192 Volume 2 Issue 1 Online Available at www.phytojournal.com Journal of Pharmacognosy and Phytochemistry Preliminary Phytochemical Screening and HPTLC
More informationJournal of Chemical and Pharmaceutical Research, 2013, 5(3): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2013, 5(3):98-104 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Preliminary phytochemical screening and HPTLC fingerprinting
More informationBotanical and phytochemical evaluation of fruits of spondias pinnata
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2015, 7 (5):276-280 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationInternational Journal of Research in Pharmacy and Science
Research Article Available online www.ijrpsonline.com ISSN: 2249 3522 International Journal of Research in Pharmacy and Science DPPH Scavenging Activity of Various Extracts of Sweet Potatoes Leaves with
More informationTHIN LAYER CHROMATOGRAPHY
THIN LAYER CHROMATOGRAPHY Thin layer chromatography is the best known technique of plant biochemistry. TLC is used for preliminary separation and determination of plant constituents. It is helpful for
More informationPhytochemical Screening of Tubers and Leaf extracts of Sagittaria sagittifolial.:newsa (Arrowhead)
International Journal of Scientific and Research Publications, Volume 7, Issue 9, September 2017 431 Phytochemical Screening of Tubers and Leaf extracts of Sagittaria sagittifolial.:newsa (Arrowhead) Anita
More informationTaraxacum officinale Herb as an Antiinflammatory
American Journal Advanced Drug Delivery www.ajadd.co.uk Taraxacum ficinale Herb as an Antiinflammatory Medicine M. Amin Mir* 1, S.S. Sawhney 1 and Manmohan Singh Jassal 2 1 R & D Division, Uttaranchal
More informationInternational Journal of Research in Cosmetic Science. Original Article
Available online at http://www.urpjournals.com International Journal of Research in Cosmetic Science Universal Research Publications. All rights reserved Original Article ANTITYROSINASE AND ANTIOXIDANT
More informationFABRICATION AND EVALUATION OF GLIMEPIRIDE CORDIA DICHOTOMA G.FORST FRUIT MUCILAGE SUSTAINED RELEASE MATRIX TABLETS
Int. J. Chem. Sci.: 7(4), 2009, 2555-2560 FABRICATION AND EVALUATION OF GLIMEPIRIDE CORDIA DICHOTOMA G.FORST FRUIT MUCILAGE SUSTAINED RELEASE MATRIX TABLETS HINDUSTAN ABDUL AHAD *, B. PRADEEP KUMAR, C.
More informationResearch Article An In-Vitro Free Radical Scavenging Activity of Methanolic Extracts of Whole Plant of Teramnus labialis (Linn.)
Research Article An In-Vitro Free Radical Scavenging Activity of Methanolic Extracts of Whole Plant of Teramnus labialis (Linn.) G. Alagumani Vasagam*, A. Kottai Muthu and R. Manavalan Department of Pharmacy,
More informationPreliminary phytochemical screening of foliar extract of Anthocephalus cadamba (Roxb.)
International Journal of Sciences & Applied Research www.ijsar.in Preliminary phytochemical screening of foliar extract of Anthocephalus cadamba (Roxb.) Basant Kumar 1, Garima Bartariya 2 and Abhishek
More informationPhytochemical screening and quantitative analysis of hexane, acetone, methanol & water extracts of Salicornia virginica by UV-spectrophotometry
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (16):52-56 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationOPTIMIZATION OF MICROWAVE-ASSISTED EXTRACTION OF BIOACTIVE COMPOUNDS FROM LEAVES AND STEMS OF THAI WATER SPINACH (Ipomoea aquatic var.
OPTIMIZATION OF MICROWAVE-ASSISTED EXTRACTION OF BIOACTIVE COMPOUNDS FROM LEAVES AND STEMS OF THAI WATER SPINACH (Ipomoea aquatic var. aquatica) Boonnet Koonchanok 1, Thadsaneeya Cheunchob 1, Nisakorn
More informationIn Vitro Antioxidant Activity of some Edibles Bearing Nutritional Value
International Journal of Biotechnology and Bioengineering Research. ISSN 2231-1238, Volume 4, Number 5 (2013), pp. 487-494 Research India Publications http://www.ripublication.com/ ijbbr.htm In Vitro Antioxidant
More informationAsian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:
Banu Rekha J and Jaykar. / Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry. 3(2), 2015, 76-81. Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and
More informationKeywords: antioxidant; extraction; paper flower; phenolic compound
PHENOLIC ANTIOXIDANTS FROM Bougainvillea SPP. Punyawatt Pintathong 1, *, Prisana Pinket 1, Monthira Papoodplook 1, Natthawut Thitipramote 1,2, Phanuphong Chaiwut 1 1 School of Cosmetic Science, Mae Fah
More informationPreparation and characterization of Aloe vera extract
Chapter 2 Preparation and characterization of Aloe vera extract 2.1. INTRODUCTION Aloe vera ethanolic extract was prepared according to Noor et.al, 2008 with few modifications. The phytochemical analysis
More informationS. Sumathi*, G.T. Iswariya, B. Sivaprabha, B. Dharani, P. Radha, and P.R. Padma
IJPSR (2013), Vol. 4, Issue 3 (Research Article) Received on 21 November, 2012; received in revised form, 11 January, 2013; accepted, 23 February, 2013 COMPARATIVE STUDY OF RADICAL SCAVENGING ACTIVITY
More informationANTI-OXIDANTANT AND PHYTOCHEMICAL SCREENING OF ACALYPHA COMMUNIS (müll. arg.) BY HPTLC FINGER PRINTING METHOD
IJCRR Section: Healthcare Sci. Journal Impact Factor 4.016 Research Article ANTI-OXIDANTANT AND PHYTOCHEMICAL SCREENING OF ACALYPHA COMMUNIS (müll. arg.) BY HPTLC FINGER PRINTING METHOD Rajasekaran S.
More informationInternational Journal of Pharma and Bio Sciences
Research Article Microbiology International Journal of Pharma and Bio Sciences ISSN 0975-6299 PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF CLITORIA TERNATEA LEAVES AGAINST STAPHYLOCOCCUS AUREUS
More informationPharmacologyonline 3: (2011) ewsletter Verma et al.
A TIDIABETIC ACTIVITY OF ETHYL ACETATE EXTRACT OF PTEROCARPUS MARSUPIUM I ALLOXA I DUCED DIABETIC RATS Prabhakar K Verma 1*, Vipan K Kamboj 2 Manoj K Mishra 3 Sudhir Ranjan 4 1* Department of Pharmaceutical
More informationStandardisation of Herbal Extract Mixture by HPTLC Method
Research Article Standardisation of Herbal Extract Mixture by HPTLC Method S. Dhanalakshmi*, Jayakumari S., Abinaya S.K, Srudhi, Elenchizeyan Dept of Pharmacognosy, School of Pharmaceutical Sciences, (VISTAS),
More informationJournal of Medicinal Plants Studies Preliminary Phytochemical Studies Of Kalanchoe pinnata (Lam.) Pers
Year: 2013, Volume: 1, Issue: 2 First page: (19) Last page: (23) ISSN: 2320-3862 Online Available at Journal of Medicinal Plants Studies Preliminary Phytochemical Studies Of Kalanchoe pinnata (Lam.) Pers
More informationEVALUATION OF TOTAL PHENOLIC, FLAVONOID CONTENT, ANTIOXIDANT AND IN VITRO ANTILITHOGENESIS ACTIVITIES OF CHIVES LEAF (Allium schoenoprasum, L.
ISSN: 0974-1496 e-issn: 0976-0083 CODEN: RJCABP http://www.rasayanjournal.com http://www.rasayanjournal.co.in EVALUATION OF TOTAL PHENOLIC, FLAVONOID CONTENT, ANTIOXIDANT AND IN VITRO (Allium schoenoprasum,
More informationA November 2015
Phytochemical Screening, Total Phenolic Content and Antioxidant Activity of Syzygium zeylanicum Leaves AZIZAH MAHMOOD Politeknik Pagoh Johor, Semambu, 25350 Kuantan, Pahang, Malaysia. Email: azizah1.poli@1govuc.gov.my
More informationJournal of Applied Pharmacy (ISSN );
203 J App Pharm Vol. 7; Issue 3: 184-210; July, 2015 Kundan et al., 2015 Original Research Article PHARMACOGNOSTIC STUDIES AND PHYSICO-CHEMICAL PROFILE OF MELISSA PARVIFLORA BENTH. Kundan Singh Bora*,
More informationFLAVONOIDS AND PHENOLIC COMPOUNDS FROM Litsea polyantha JUSS. BARK
FLAVONOIDS AND PHENOLIC COMPOUNDS FROM Litsea polyantha JUSS. BARK Manik Ghosh 1 *, Barij N. Sinha 1, Julio A. Seijas 2 *, M. Pilar Vázquez-Tato 2, Xesús Feás 2 1 Department of Pharmaceutical Sciences
More informationChapter 2 Biochemical changes and antioxidant activity of elephant- foot yam corm during development
Chapter 2 Biochemical changes and antioxidant activity of elephant- foot yam corm during development Introduction The corm of elephant-foot yam is used as vegetable and also as a major ingredient in various
More informationKeywords- Hibiscus rosa- sinensislinn., phytochemical, extract.antibacterial, axillary solitary, companulate.
GLOBAL JOURNAL OF ENGINEERING SCIENCE AND RESEARCHES COMPARATIVE PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF ETHANOLIC AND WATER FLOWER EXTRACTS OF HIBISCUS ROSASINENSIS LINN. (HIBISCUS RED)
More informationAntioxidant Activity and Anticancer Study on Phytochemicals Extract from Tubers of Gloriosa superba against Human Cancer Cell (Hep-G2)
Antioxidant Activity and Anticancer Study on Phytochemicals Extract from Tubers of Gloriosa superba against Human Cancer Cell (Hep-G2) Simon SE 1* and Jayakumar FA 2 1 Department of Biotechnology, Bharathidasan
More informationScience & Technologies ANTIOXIDANT ACTIVITY AND SECONDARY METABOLITES IN DIFFERENT EXTRACTS OF EUPHRASIA OFFICINALIS L. GROWING IN BULGARIA
ANTIOXIDANT ACTIVITY AND SECONDARY METABOLITES IN DIFFERENT EXTRACTS OF EUPHRASIA OFFICINALIS L. GROWING IN BULGARIA Dimitrova M., Hristova L., Damianova E., Yordanova Y., Petrova N., V.Kapchina-Toteva*
More informationJOURNAL OF SCIENTIFIC & INNOVATIVE RESEARCH
Volume 1 Issue 3 2012 Available online at: JOURNAL OF SCIENTIFIC & INNOVATIVE RESEARCH Preliminary Phytochemical analysis of Leaf Extract of Alternanthera Brasiliana Ruchi Singh *1, Sanchida Sakida 1,
More informationComparative HPTLC Analysis of Leaves of Allium cepa L., Ficus carica L. and Ziziphus mauritiana L. with Standard Quercetin
Available online on www.ijppr.com International Journal of Pharmacognosy and Phytochemical Research 2017; 9(5); 739-743 DOI number: 10.25258/phyto.v9i2.8157 ISSN: 0975-4873 Research Article Comparative
More informationFirst Order Derivative Spectrophotometric Method for Estimation of Melatonin in Bulk and Pharmaceutical Dosage Form
Human Journals Research Article August 2017 Vol.:10, Issue:1 All rights are reserved by Bhusnure O.G.et al. First Order Derivative Spectrophotometric Method for Estimation of Melatonin in Bulk and Pharmaceutical
More informationISOLATION AND CHARACTERIZATION OF HESPERIDIN FROM ORANGE PEEL
Indo American Journal of Pharmaceutical Research, 2013 ISSN NO: 2231-6876 Journal home page: http:///index.php/en/ INDO AMERICAN JOURNAL OF PHARMACEUTICAL RESEARCH ISOLATION AND CHARACTERIZATION OF HESPERIDIN
More informationPhytochemical and antioxidant properties of some Cassia species
Phytochemical and antioxidant properties of some Cassia species Firdose R. Kolar a *, Chaya L. Gogi a, Mairunisabegum M. Khudavand a, Meera S. Choudhari a and Sindhu B. Patil a a Department of Botany,
More informationPhytochemical Investigation and Evaluation of Antioxidant Activity of Dalbergia sissoo Bark
Human Journals Research Article June 217 Vol.:9, Issue:3 All rights are reserved by ANUSHA GOVINDULA et al. Phytochemical Investigation and Evaluation of Antioxidant Activity of Dalbergia sissoo Bark Keywords:
More informationPhytochemical Analysis and Antimicrobial Activities of Carissa carandas L.
Universities Research Journal 2011, Vol. 4, No. 1 Phytochemical Analysis and Antimicrobial Activities of Carissa carandas L. Aye Aye Naing Abstract The plant Carissa carandas L. locally known as Khan-pin,
More informationPharmacognostic, Phytochemical and Physicochemical Study of Ficus arnottiana Miq. Leaves (Moraceace)
Available online on www.ijppr.com International Journal of Pharmacognosy and Phytochemical Research 2016; 8(1); 193-198 Research Article ISSN: 0975-4873 Pharmacognostic, Phytochemical and Physicochemical
More information3. RESULTS AND DISCUSSION
3. RESULTS AND DISCUSSION 3.1. PHYTOCHEMICAL INVESTIGATIONS OF F.RACEMOSA BARK The course powder of Ficus racemosa was sequentially extracted by Pet. Ether, Ethyl acetate along with Alcohol by Soxhlet
More informationIN VITRO ANTICANCER ACTIVITY OF FLOWER EXTRACTS OF COUROUPITA GUIANENSIS
CHAPTER 3 IN VITRO ANTICANCER ACTIVITY OF FLOWER EXTRACTS OF COUROUPITA GUIANENSIS 3. INTRODUCTION Plants are the basic source of knowledge of modern medicine. Almost all the parts of the plant, namely
More informationMethod Development and Validation of Emtricitabine in Bulk by UV Spectroscopy
Human Journals Research Article February 2019 Vol.:14, Issue:3 All rights are reserved by Gandi Anusha et al. Method Development and Validation of Emtricitabine in Bulk by UV Spectroscopy Keywords: Emtricitabine,
More informationChandan Prasad.et.al. Int. Journal of Engineering Research and Application ISSN : , Vol. 7, Issue 9, ( Part -6) September 2017, pp.
RESEARCH ARTICLE OPEN ACCESS Antibacterial properties of Unripened Fruits of Trapa natans, Annona squamosa, Ficus carica and Anthocephalus cadamba against Pathogenic Gram Positive and Negative Bacteria.
More informationINTERNATIONAL RESEARCH JOURNAL OF PHARMACY ISSN Research Article
INTERNATIONAL RESEARCH JOURNAL OF PHARMACY www.irjponline.com ISSN 223 87 Research Article EVALUATION OF ANTIOXIDANT ACTIVITY OF CORCHORUS AESTUANS LINN LEAVES EXTRACTS Patel Rashmika P. 1 *, Patel Manish
More informationThis chapter deals with the evaluation of alpha amylase inhibitory
This chapter deals with the evaluation of alpha amylase inhibitory activity of different extracts isolated from leaves of Aloe vera L. and leaves of Azadiracta indica A Juss. collected from Bharatpur and
More informationScholars Research Library
Available online at www.scholarsresearchlibrary.com J. Nat. Prod. Plant Resour., 2013, 3 (2):86-91 (http://scholarsresearchlibrary.com/archive.html) ISSN : 2231 3184 CODEN (USA): JNPPB7 Phytochemical Screening
More informationScholars Research Library. Phytochemical studies of Svensonia hyderobadensis (walp.) Mold: A rare medicinal plant
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2011: 3 (4) 51-55 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationVol-3, Issue-4, Suppl-1, Nov 2012 ISSN: Patel et al PHARMA SCIENCE MONITOR
PHARMA SCIENCE MONITOR AN INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR ALOE POLYSACCHARIDES AS A MEASURE OF ALOE VERA QUALITY IN HERBAL FORMULATION
More informationPelagia Research Library. Study of antioxidant activity of ethanolic extract of fresh Eichhornia crassipes (Mart.)Solms
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2012, 3 (2):271-277 ISSN: 0976-8688 CODEN (USA): PSHIBD Study of antioxidant activity of ethanolic extract of fresh Eichhornia crassipes
More informationPhytochemical screening and estimation of total phenolics and total flavonoid content of Cocos nucifera endocarp
World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.org/ Original
More informationIJPRD, 2013; Vol 5(03): November-2013 ( ) International Standard Serial Number
IJPRD, 2013; Vol 5(03): November-2013 (001 006) International Standard Serial Number 0974 9446 --------------------------------------------------------------------------------------------------------------------------------------------------
More information------------------------------------------------------------------------------------------------------------------------ HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC METHOD FOR QUANTIFICATION OF PIPERINE
More informationThe Journal of Phytopharmacology
Volume 1 Issue 3 2012 The Journal of Phytopharmacology (Pharmacognosy and Phytomedicine Research) Comparing of antioxidant and DPPH induced free radical scavenging activity of Sesbania grandiflora and
More informationResearch Article In-vitro antioxidant activity of Cassia auriculata Leaves
Research Article In-vitro antioxidant activity of Cassia auriculata Leaves R. Radha 1*, MVV. Prasad 2, A. Lakshmi Devi 1, R. Bhanu Murthy, M. Ravi Kumar 1, B. Sitaram 3 and D. Ranganayakulu 1 1 Sri Padmavathi
More informationSUPPLEMENTARY MATERIAL
SUPPLEMENTARY MATERIAL Identification and in vitro antioxidant activities of phenolic compounds isolated from Cynoglossum cheirifolium L. Bensaid Ilhem 1, Atik Bekkara Fawzia 1, El Haci Imad Abdelhamid
More informationSequential Extraction of Plant Metabolites
ISSN: 2319-7706 Volume 4 Number 2 (2015) pp. 33-38 http://www.ijcmas.com Original Research Article Sequential Extraction of Plant Metabolites Shankar L. Laware* PG. Department of Botany, Fergusson College
More informationExtraction and Standardization of Medicinal Herbal Products
Extraction and Standardization of Medicinal Herbal Products By: Dr Masoud Sadeghi Dinani Department of Pharmacognosy Isfahan University of Medical Sciences Medicinal Plants About 80% of all the world s
More informationCHEM104 Exp. 9 Phytochemical Antioxidants with Potential Benefits in Foods Part I. 1
EM104 Exp. 9 Phytochemical Antioxidants with Potential Benefits in Foods Part I. 1 ame People have known and believed for a long time that eating certain foods is good for their health. owever, it is difficult
More informationPhytochemical Analysis of Alfalfa (Medicago sativa) Seed Extract by Soxhlet Extraction Using Different Solvents
American Journal of Advanced Drug Delivery www.ajadd.co.uk Original Article Phytochemical Analysis of Alfalfa (Medicago sativa) Seed Extract by Soxhlet Extraction Using Different Solvents G. Sheela Joy
More informationSummary. and. conclusion
Summary and conclusion The focus of the study was to investigate nutritional and medicinal values of ten wild edible fruits in Kolhapur district viz. Ficus racemosa, Elaeagnus conferta, Antidesma ghasembilla,
More informationHPTLC Method for estimation of Gallic acid and Rutin in Haritaki -An Ayurvedic Formulation
International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol. 3, No.2, pp 986-999, April-June 2011 HPTLC Method for estimation of Gallic acid and Rutin in Haritaki -An Ayurvedic
More informationImpact factor: 3.958/ICV: 4.10 ISSN:
Impact factor: 3.958/ICV: 4.10 ISSN: 0976-7908 402 Pharma Science Monitor 9(2), Apr-Jun 2018 PHARMA SCIENCE MONITOR AN INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES Journal home page: http://www.pharmasm.com
More informationResearch Article Derivative Spectrophotometric Method for Estimation of Metformin Hydrochloride in Bulk Drug and Dosage Form
Research Article Derivative Spectrophotometric Method for Estimation of Metformin Hydrochloride in Bulk Drug and Dosage Form Gowekar NM, Lawande YS*, Jadhav DP, Hase RS and Savita N. Gowekar Department
More informationIsolation and characterization of stigmast- 5-en-3β-ol (β-sitosterol) from the leaves of Hygrophila spinosa T. Anders
Isolation and characterization of stigmast- 5-en-3β-ol (β-sitosterol) from the leaves of Hygrophila spinosa T. Anders Arjun Patra 1*, S. Jha 2, P.N. Murthy 3, Manik 2, A. Sharone 2 1 College of Pharmacy,
More informationGlobal College of Pharmacy, Kahnpur Khui, Tehsil Anandpur Sahib, Distt.- Ropar, Punjab, India
IJPSR (2012), Vol. 3, Issue 09 (Research Article) Received on 19 May, 2012; received in revised form 25 June, 2012; accepted 27 August, 2012 IN-VITRO EVALUATION OF TWO MARKETED BRANDS OF PARACETAMOL TABLETS
More informationPreliminary Phytochemical Screening and HPTLC Fingerprinting of Bark Extracts of Symplocos racemosa
2013; 2 (3): 4549 ISSN 22784136 ISSN 23498234 JPP 2013; 2 (3): 4549 2013 AkiNik Publications Received: 1872013 Accepted: 0982013 Preliminary Phytochemical Screening and HPTLC Fingerprinting of Bark s of
More informationEvaluation of HPTLC Fingerprints of Flavonoids and Antioxidant Activity of Selected Medicinal Plants of Lamiaceae Family
Available online on www.ijppr.com International Journal of Pharmacognosy and Phytochemical Research 2015; 7(2); 240-245 Research Article ISSN: 0975-4873 Evaluation of HPTLC Fingerprints of Flavonoids and
More informationPhytochemical,Screening,of,Various,Extracts,of,Root,of,Withania, Somnifera*(L)*Dunal*
Archieves*of*Business*Research* *Vol.3,*No.2* Publication*Date:March25,2015 DOI:10.14738/abr.32.823. Vinotha,S.,Thabrew,I.,&Ranjani,S.S.(2015).PhytochemicalScreeningofVariousExtractsofRootofWithaniaSomnifere
More information