SAFETY DATA SHEET FLOW CELL BUFFER

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1 Revision Date 03/2014 SAFETY DATA SHEET FLOW CELL BUFFER According to Regulation (EU) No 453/2010 SECTION 1: IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING 1.1. Product identifier Product name FLOW CELL BUFFER Container size 750 µl 1.2. Relevant identified uses of the substance or mixture and uses advised against Identified uses Laboratory Reagent. For research purposes only Details of the supplier of the safety data sheet Supplier Oxford Nanopore Technologies Ltd. Edmund Cartwright House, 4 Robert Robinson Avenue, Oxford Science Park, Oxford, OX4 4GA UK T: +44 (0) F: +44 (0) W: E: support@nanoporetech.com 1.4. Emergency telephone number +44 (0) (Mon - Fri 09:00-17:00 h) SECTION 2: HAZARDS IDENTIFICATION 2.1. Classification of the substance or mixture Classification (EC 1272/2008) Classification (1999/45/EEC) R32. Physical and Chemical Hazards Not classified. Human health Environment EUH032 Not classified. The Full Text for all R-Phrases and Hazard Statements are Displayed in Section 16. Human health Warning - this preparation contains a substance not yet tested completely. Contact with acids liberates very toxic gas. Environment The product contains a substance which is harmful to aquatic organisms and which may cause long term adverse effects in the aquatic environment Label elements Label In Accordance With (EC) No. 1272/2008 No pictogram required. Precautionary Statements Supplemental label information 2.3. Other hazards P280 EUH032 Wear protective clothing, gloves, eye and face protection. Contact with acids liberates very toxic gas. This product does not contain any PBT or vpvb substances. SECTION 3: COMPOSITION/INFORMATION ON INGREDIENTS 3.2. Mixtures 1 / 12

2 POTASSIUM FERROCYANIDE 5-10% CAS-No.: EC No.: Classification (EC 1272/2008) EUH032 Aquatic Chronic 3 - H412 Classification (67/548/EEC) R52/53,R32. POTASSIUM FERRICYANIDE 1-5% CAS-No.: EC No.: Classification (EC 1272/2008) EUH032 Classification (67/548/EEC) R32. The Full Text for all R-Phrases and Hazard Statements are Displayed in Section 16. SECTION 4: FIRST AID MEASURES 4.1. Description of first aid measures General information Warning - this preparation contains a substance not yet tested completely. Get medical advice/attention if you feel unwell. Contact with acids liberates very toxic gas. IF exposed or concerned: Get medical advice/attention. Inhalation Due to the small packaging the risk of inhalation is minimal. Move into fresh air and keep at rest. Get medical attention if any discomfort continues. Ingestion Get medical attention if any discomfort continues. Skin contact Remove contaminated clothing. Wash skin thoroughly with soap and water for several minutes. Get medical attention promptly if symptoms occur after washing. Eye contact Make sure to remove any contact lenses from the eyes before rinsing. Promptly wash eyes with plenty of water while lifting the eye lids. Continue to rinse for at least 15 minutes. Get medical attention if any discomfort continues Most important symptoms and effects, both acute and delayed Inhalation No specific symptoms noted. Ingestion Not known. May cause discomfort if swallowed. Skin contact No specific symptoms noted. Eye contact Spray in the eyes: May cause temporary eye irritation Indication of any immediate medical attention and special treatment needed The severity of the symptoms described will vary dependant of the concentration and the length of exposure. Warning - this preparation contains a substance not yet tested completely. Contact with acids liberates very toxic gas. Hydrogen cyanide (HCN). SECTION 5: FIREFIGHTING MEASURES 5.1. Extinguishing media Extinguishing media This product is not flammable. Use fire-extinguishing media appropriate for surrounding materials. Unsuitable extinguishing media Do not use water jet as an extinguisher, as this will spread the fire Special hazards arising from the substance or mixture Hazardous combustion products Fire creates: Carbon monoxide (CO). Carbon dioxide (CO2). Hydrocarbons. Hydrogen cyanide (HCN). 2 / 12

3 Unusual Fire & Explosion Hazards No unusual fire or explosion hazards noted. Specific hazards Contact with acids liberates very toxic gas. FLOW CELL BUFFER 5.3. Advice for firefighters Special Fire Fighting Procedures Contact with acids liberates very toxic gas. Warning - this preparation contains a substance not yet tested completely. Avoid contact with skin and eyes. Protective equipment for fire-fighters Self contained breathing apparatus and full protective clothing must be worn in case of fire. SECTION 6: ACCIDENTAL RELEASE MEASURES 6.1. Personal precautions, protective equipment and emergency procedures Wear protective clothing as described in Section 8 of this safety data sheet Environmental precautions Avoid discharge to the aquatic environment Methods and material for containment and cleaning up In case of spills, beware of slippery floors and surfaces. Wear necessary protective equipment. CAUTION! Contact with acids liberates very toxic gas. Absorb spillage with non-combustible, absorbent material. Transfer to a container for disposal. For waste disposal, see section Reference to other sections Wear protective clothing as described in Section 8 of this safety data sheet. See section 11 for more detailed information on health effects and symptoms. For waste disposal, see section 13. SECTION 7: HANDLING AND STORAGE 7.1. Precautions for safe handling Read and follow manufacturer's recommendations. Wear protective clothing as described in Section 8 of this safety data sheet. Do not handle broken packages without protective equipment. Avoid contact with skin and eyes. Do not eat, drink or smoke when using the product. Wash hands thoroughly after handling. Avoid contact with acids Conditions for safe storage, including any incompatibilities Protect from freezing and direct sunlight. Store at temperatures not exceeding 4 C. Store away from: Acids Specific end use(s) The identified uses for this product are detailed in Section 1.2. SECTION 8: EXPOSURE CONTROLS/PERSONAL PROTECTION 8.1. Control parameters Ingredient Comments No exposure limits noted for ingredient(s) Exposure controls Process conditions Provide eyewash station. Engineering measures Provide adequate general and local exhaust ventilation. Respiratory equipment Respiratory protection may be required. Contact with acids liberates very toxic gas. Wear full face mask supplied with: Type: ABEK [ EN 14387:2004 ] Hand protection Protective gloves must be used if there is a risk of direct contact or splash. The most suitable glove must be chosen in consultation with the gloves supplier, who can inform about the breakthrough time of the glove material. Eye protection Wear approved safety goggles. 3 / 12

4 Other Protection Wear suitable protective clothing as protection against splashing or contamination. Hygiene measures Wash hands after handling. When using do not eat, drink or smoke. Wash contaminated clothing before reuse. Environmental Exposure Controls Avoid discharge to the aquatic environment. SECTION 9: PHYSICAL AND CHEMICAL PROPERTIES 9.1. Information on basic physical and chemical properties Appearance Colour Odour Solubility Initial boiling point and boiling range ( C) Melting point ( C) Relative density Vapour density (air=1) Vapour pressure Evaporation rate ph-value, Conc. Solution Viscosity Decomposition temperature ( C) Odour Threshold, Lower Odour Threshold, Upper Flash point ( C) Auto Ignition Temperature ( C) Flammability Limit - Lower(%) Flammability Limit - Upper(%) Partition Coefficient (N-Octanol/Water) Explosive properties Not considered to be explosive. Oxidising properties Does not meet the criteria for oxidising Other information Not known. Liquid Colourless to pale yellow. No characteristic odour. Soluble in water mm Hg ~0 760 mm Hg SECTION 10: STABILITY AND REACTIVITY Reactivity Reaction with: Acids Chemical stability Stable under normal temperature conditions and recommended use. Stable under the prescribed storage conditions. 4 / 12

5 10.3. Possibility of hazardous reactions Not known. Hazardous Polymerisation Will not polymerise Conditions to avoid Avoid heat. Protect from freezing and direct sunlight Incompatible materials Materials To Avoid Contact with acids liberates very toxic gas Hazardous decomposition products Not known. SECTION 11: TOXICOLOGICAL INFORMATION Information on toxicological effects Toxicological information Warning - this preparation contains a substance not yet tested completely. Other Health Effects Contact with acids liberates very toxic gas. Hydrogen cyanide (HCN). Acute toxicity: Acute Toxicity (Oral LD50) Acute Toxicity (Dermal LD50) Acute Toxicity (Inhalation LC50) Skin Corrosion/Irritation: Serious eye damage/irritation: Respiratory or skin sensitisation: Respiratory sensitisation Skin sensitisation Germ cell mutagenicity: Genotoxicity - In Vitro Carcinogenicity: Carcinogenicity Reproductive Toxicity: Reproductive Toxicity - Fertility 5 / 12

6 Reproductive Toxicity - Development FLOW CELL BUFFER Specific target organ toxicity - single exposure: STOT - Single exposure Specific target organ toxicity - repeated exposure: STOT - Repeated exposure Aspiration hazard: Not anticipated to present an aspiration hazard based on chemical structure. Toxicological information on ingredients. 6 / 12

7 Toxicological information CAUTION! Contact with acids liberates very toxic gas. Hydrogen cyanide (HCN). Acute toxicity: Acute Toxicity (Oral LD50) 3613 mg/kg Rat Acute Toxicity (Dermal LD50) Acute Toxicity (Inhalation LC50) > 1000 mg/m3 Rat 4 hours Limited information on study available. No reported deaths occurred. Skin Corrosion/Irritation: Dose (Dose and duration unstated) Rabbit Not irritating. Serious eye damage/irritation: Slightly Irritating. Respiratory or skin sensitisation: Respiratory sensitisation Skin sensitisation Guinea pig maximization test (GPMT): Guinea Pig Not Sensitising. Germ cell mutagenicity: Genotoxicity - In Vitro DNA damage and/or repair: Negative. Genotoxicity - In Vivo Carcinogenicity: Carcinogenicity (Dose and duration unstated) Oral Rat Is not expected to be carcinogenic, based on results of related chemicals in animal studies Based on available data the classification criteria are not met. Reproductive Toxicity: Reproductive Toxicity - Fertility Reproductive Toxicity - Development Teratogenicity: NOAEL =<0.036 ppm Inhalation. Rat 7 / 12

8 Delivered in aerosol form, 24 hours a day for an unknown duration. Insufficient data presented and test not conducted to OECD guidelines. Data lacking. Specific target organ toxicity - single exposure: STOT - Single exposure Not classified as a specific target organ toxicant after a single exposure. Specific target organ toxicity - repeated exposure: STOT - Repeated exposure Not classified as a specific target organ toxicant after repeated exposure. Aspiration hazard: Viscosity Not anticipated to present an aspiration hazard based on chemical structure. 8 / 12

9 Toxicological information CAUTION! Contact with acids liberates very toxic gas. Hydrogen cyanide (HCN). Acute toxicity: Acute Toxicity (Oral LD50) 2970 mg/kg Mouse Supplier Safety Data Sheet Acute Toxicity (Dermal LD50) Acute Toxicity (Inhalation LC50) Skin Corrosion/Irritation: Data lacking. Serious eye damage/irritation: Data lacking. Respiratory or skin sensitisation: Respiratory sensitisation Skin sensitisation Germ cell mutagenicity: Genotoxicity - In Vitro Genotoxicity - In Vivo Carcinogenicity: Carcinogenicity Reproductive Toxicity: Reproductive Toxicity - Fertility Reproductive Toxicity - Development Specific target organ toxicity - single exposure: STOT - Single exposure Not classified as a specific target organ toxicant after a single exposure. Specific target organ toxicity - repeated exposure: STOT - Repeated exposure Not classified as a specific target organ toxicant after repeated exposure. 9 / 12

10 Aspiration hazard: Viscosity Not anticipated to present an aspiration hazard based on chemical structure. SECTION 12: ECOLOGICAL INFORMATION Ecotoxicity The product contains a substance which is harmful to aquatic organisms and which may cause long term adverse effects in the aquatic environment Toxicity Acute Fish Toxicity Not considered toxic to fish. Ecological information on ingredients. Acute Toxicity - Fish LC50 96 hours 19 mg/l Poecilia reticulata (Guppy) LC50 96 hours > 100 mg/l Pimephales promelas (Fat-head Minnow) Acute Toxicity - Aquatic Invertebrates EC50 96 hours 32 mg/l Daphnia magna Acute Toxicity - Microorganisms NOEC ~ 47 mg/l Activated sludge Acute Toxicity - Fish LC50 96 hours 869 mg/l Onchorhynchus mykiss (Rainbow trout) Supplier Safety Data Sheet Acute Toxicity - Aquatic Invertebrates EC50 48 hours 549 mg/l Daphnia magna Supplier Safety Data Sheet Persistence and degradability Degradability There are no data on the degradability of this product. Ecological information on ingredients. Degradability Not Applicable - Inorganic chemical. Decomposition products: Hydrogen cyanide (HCN). Iron salts. Degradability Not Applicable - Inorganic chemical Bioaccumulative potential Bioaccumulative potential No data available on bioaccumulation. Partition coefficient 10 / 12

11 Ecological information on ingredients. FLOW CELL BUFFER Bioaccumulative potential No data available on bioaccumulation. Bioaccumulative potential No data available on bioaccumulation Mobility in soil Mobility: The product is soluble in water. Ecological information on ingredients. Mobility: Soluble in water. Mobility: Soluble in water Results of PBT and vpvb assessment This product does not contain any PBT or vpvb substances. Ecological information on ingredients. Not Classified as PBT/vPvB by current EU criteria. Not Classified as PBT/vPvB by current EU criteria Other adverse effects Contact with acids liberates very toxic gas. Ecological information on ingredients. None known. None known. SECTION 13: DISPOSAL CONSIDERATIONS Waste treatment methods When handling waste, consideration should be made to the safety precautions applying to handling of the product. Contact with acids liberates very toxic gas. Residues and empty containers should be taken care of as hazardous waste according to local and national provisions. SECTION 14: TRANSPORT INFORMATION General The product is not covered by international regulation on the transport of dangerous goods (IMDG, IATA, ADR/RID) UN number UN proper shipping name Transport hazard class(es) Transport Labels No transport warning sign required. 11 / 12

12 14.4. Packing group Environmental hazards Environmentally Hazardous Substance/Marine Pollutant No Special precautions for user Transport in bulk according to Annex II of MARPOL73/78 and the IBC Code SECTION 15: REGULATORY INFORMATION Safety, health and environmental regulations/legislation specific for the substance or mixture Guidance Notes Workplace Exposure Limits EH40. EU Legislation Regulation (EC) No 1272/2008 of the European Parliament and of the Council of 16 December 2008 on classification, labelling and packaging of substances and mixtures, amending and repealing Directives 67/548/EEC and 1999/45/EC, and amending Regulation (EC) No 1907/2006 with amendments Chemical Safety Assessment No chemical safety assessment has been carried out. SECTION 16: OTHER INFORMATION General information Only trained personnel should use this material. Revision Comments This is first issue. Revision Date 03/2014 Risk Phrases In Full R32 R52/53 Hazard Statements In Full EUH032 H412 Contact with acids liberates very toxic gas. Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment. Contact with acids liberates very toxic gas. Harmful to aquatic life with long lasting effects. 12 / 12

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