PURAMEX ZN. For an all-day shine-free appearance CORBION.COM/PERSONALCARE

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1 PURAMEX ZN For an all-day shine-free appearance CORBION.COM/PERSONALCARE 11/3/2014

2 Sebum Sebum is an oily substance that is secreted by sebaceous glands in the skin, which are usually connected to the hair follicle. In the glands, sebum is produced within specialized cells and is released when the cells reach maturity. Sebum mostly consists of triglyceride oils, wax, fatty acids and squalene. It is essential to lubricate hair and keep skin supple and plays an important role in maintaining the protective acid mantle of the skin. Schematic view of hair follicle and sebaceous gland /3/2014

3 Oily skin/scalp Oily skin is caused by excess sebum production (also know as seborrhoea). It is characterized by shininess, especially in the so-called T-zone, blemishes and pimples. Oily skin is especially susceptible to clogged pores, blackheads, and buildup of dead skin cells on the surface of the skin. Men tend to have oilier skin than women. Excess sebum production on the scalp can also cause oily hair /3/2014

4 Sebum control Zinc lactate as zinc source for sebum control Zinc is well-known to inhibit the enzyme 5 -reductase*, which is involved in converting testosterone (an androgen hormone) into dihydrotestosterone (DHT). Testosterone 5 -reductase DHT Sebaceous gland Receptor DHT binds to receptors on the sebaceous glands and this stimulates the sebaceous glands to produce increased quantities of sebum. By inhibiting 5 -reductase, sebum production may be reduced. *D. Stamatiadis et al. British Journal of Dermatology (1988) 119, /3/2014

5 PURAMEX ZN efficient Zn source Zinc lactate contains a higher percentage of Zn than other popular Zn sources, such as Zinc PCA and Zinc Gluconate. Zn source MW (anhydrous) % Zn Zinc Lactate % Zinc PCA % Zinc Gluconate % PCA = pyrrolidone carboxylic acid In addition zinc lactate also provides lactate, an essential component of the natural moisturizing factor of the skin (NMF), for additional moisturizing benefits PURAMEX ZN is an efficient Zn source with added moisturizing benefits /3/2014

6 PURAMEX ZN short term sebum control effect Clinical study on sebum reduction efficacy of PURAMEX ZN in a no-rinse facial cleansing lotion. Ingredient INCI w/w % Water Aqua 61.5 Cremerol 1 HMG Hydrogenated milk glycerides 0.5 Captex Propylene Glycol Dicaprate 3.0 Clinical study 30 female volunteers Age Oily to slightly oily skin, especially around T-zone Glucamate 3 SSE-20 PEG-20 Methyl Glucose Sesquistearate 6.0 Cetyl Alcohol Cetyl Alcohol 4.0 PURAMEX ZN Zinc Lactate 2.0 Lubrajel 3 MS Actiwash 4 Glycerin (and) Glyceryl Acrylate/Acrylic Acid Copolymer (and) Propylene Glycol Butylene Glycol (and) Water (and) Urtica Dioica (Nettle) Extract (and) Equisetum Hyemale Extract (and) Camellia Oleifera Leaf Extract Protocol 1 Week wash out period Measurement of sebum level by Sebumeter before cleansing and 30 mins, 1hr, 2 hrs and 4 hrs after cleansing Test product on one half of the face, control (cleanser base) on the other half of the face. 1 Dow 3 Lubrizo 2 Abitecl 4 Active Organics /3/2014

7 PURAMEX ZN sebum control Clinical study on short term sebum reduction efficacy of 2% PURAMEX ZN in a (norinse) facial cleansing lotion. Sebum level (arbitrary units) Before 4 hrs -24% * Placebo 2% PURAMEX ZN *Significant vs placebo P<0.05 Student T -Test At 2% use level, PURAMEX ZN significantly reduced sebum levels up to 24% after 4 hours /3/2014

8 PURAMEX ZN long term sebum control effect Clinical study on sebum reduction efficacy of PURAMEX ZN in a facial gel-cream Ingredient INCI w/w % Cetiol 1 C5 Coco Caprylate/Caprate 5.0 IPP 1 Isopropyl Palmitate 4.0 CM50 Cyclopentasiloxane 2.0 Montanov 2 L C14-C22 Alcohol (and) C12-C20 Alkyl Glycoside Myritol Caprylic/Capric Triglyceride 1.0 Cutina 1 GMS-V Glyceryl Stearate 2.0 Water Aqua Ad 100 Glycerin Glycerin Clinical study 20 volunteers Age Very oily skin (sebum level 140 μg/cm² verified by Sebumeter ), Protocol 1 Week wash out period Application of product 2x day during 4 weeks Measurement of sebum level by Sebumeter Sepinov 2 EMT 10 Hydroxyethyl Acrylate / Sodium Acryloyldimethyl Taurate Copolymer PURAMEX ZN Zinc Lactate Preservative ph adjuster To ph 5 q.s 1 BASF 2 Seppic /3/2014

9 PURAMEX ZN sebum control Clinical study on long term sebum reduction efficacy of 1% PURAMEX ZN in a (leaveon) gel-cream on the forehead 0-10 Sebum reduction in % vs D0 D7 D14 D21 D At 1% use level, PURAMEX ZN significantly reduced sebum levels >35% after 4 weeks use /3/2014

10 PURAMEX ZN benefits Sebum control Reducing skin shine NMF constituent included Efficient zinc source Biobased Controlling oily skin, while maintaining hydration For an all-day shine-free appearance /3/2014

11 PURAMEX ZN applications Oily skin care products Skin care products to reduce skin shine Oil controlling make-up or BB creams Products for skin with a tendency to acne Teenage skin care Men s care Oily scalp As complementary ingredient in anti-dandruff products /3/2014

12 PURAMEX ZN product characteristics PURAMEX ZN is the soluble zinc salt of natural L-Lactic acid, which is produced by fermentation from carbohydrates. INCI: Appearance: Zinc Lactate white powder Recommended use level: 0,5-2% Origin: natural fermentation ECOCERT approved* *Raw material approved by ECOCERT GREENLIFE conform the ECOCERT Natural and Organic Cosmetics Standard /3/2014

13 ZINC LACTATE IN ORAL CARE Antibacterial CORBION.COM/PERSONALCARE 11/3/2014

14 PURAMEX ZN - benefits Antibacterial properties antibacterial and anti halitosis Excellent solubility Good Zinc source Easy to formulate (mouth rinse and toothpaste) Biobased /3/2014

15 Zinc lactate in Oral Care Anti-plaque properties in dentifrices (Hanke 1940; Shah 1982) Zinc is one of the minor elements present in human dental enamel and it is a micronutrient, essential for the growth of microorganisms present in the oral cavity at trace levels Elevated concentrations of zinc may have inhibitory effects on bacterial growth (Gallagher and Cutress 1977; Babich and Stotzky 1978) Zinc is an effective bacteriostatic antimicrobial agent against caries-associated Streptococcus mutans and various oral bacteria that are associated with gingivitis such as Fusobacterium nucleatum and Prevotella intermedia (Marquis et al. 2005) Studies have shown that zinc has an antihalitosis effect (Yaegaki and Suetaka 1989; Waler 1997) Waler et al reported that the mechanism of action zinc involved in the inhibition of volatile sulphur compounds (VSC) production responsible for malodour (halitosis) was due to its ability to form stable mercaptides (metal thiolate) with the substrate of the VSC in the oral cavity (Waler 1997; Young et al. 2001) /3/2014

16 Test set-up Corbion in house Zn lactate antimicrobial tests EN1276, dirty conditions Contact time: 5 min 2 / 4 / 8 / 24 hours Tested specimen: S. aureus and E. hirae (both Gr+) Samples: compositions Sodium Lauryl Sulfate (35%) 2.86 PURAMEX ZN 85% HCl 5% To ph 5.5 Water Till /3/2014

17 Results 0.5% PURAMEX ZN + 1% SLS is sufficient to kill within 2 hours contact time at ph 5.5 ZnL for colgate 5 min ZnL for colgate 2 hour ZnL for colgate 4 hour ZnL for colgate 8 hour ZnL for colgate 24 hour code ph EH SA EH SA EH SA EH SA EH SA A. Zinc lactate 0.5% 5.49 <3.86 <3.95 >5 5.4 >5 5.0 >5 >5 >5 >5 B. Zinc lactate 1% 5.53 <3.86 <3.95 > >5 >5 >5 >5 C. Zinc lactate 2% 5.54 <3.86 <3.95 > >5 >5 >5 > /3/2014

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