Breeding in the past. Seedling were the main source for the cultivars today. Spontaneous mutations on branches were used to select new cultivars.

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1 Origin of citrus

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3 Breeding in the past Seedling were the main source for the cultivars today. Spontaneous mutations on branches were used to select new cultivars.

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5 Present breeding The current breeding projects are based on crossing and Selection: 1.Crossing selecion and inducing mutation for seedlessness. 2.Crossing for seedless triploids.

6 ARO Citrus Breeding Program A. New flavors and colors B. Easy peeling C. Early and Late Ripening Seasons D. Seedlessness C. High yield and low alternate bearing- more then 30 tons per hectare E. Long shelf life F. Pests resistance G. Dwarfing

7 INDUCING MUTATIONS A technique used to thoroughly extract seeds Before (with seeds): After (seedles): SEEDLESS

8 Breeding in the future Developing molecular markers for important traits such as: ripening season, alternaria, seedlessness, etc. The selection will start at the net house by sampling DNA from the seedling following by PCR. Developing biotechnology approaches for transforming genes of important traits into citrus cultivars.

9 Current projects A. Developing molecular markers for parents identification and alternaria resistance. B. Transformation of genes for drought resistance.

10 Germinating in the dark in sterile condition

11 Infecting with agrobacterium

12 Grafting the trasformants

13 Growing in transgenic greenhouse

14 Expression of reporting gene in the stomata

15 KEDEM Ripening Season - September-November Total soluble solids (TSS): 14.9% Acid content: 1.02% TSS/acid ratio: alternaria: resistant - Seedless - Early ripening

16 Rishonit RIPENING SEASON - OCTOBER-DECEMBER TOTAL SOLUBLE SOLIDS (TSS): 16.3% ACID CONTENT: 1.0% TSS/ACID RATIO: 16.3 alternaria: resistant - Seedless - Early ripening

17 ORIT Ripening Season January-February Total soluble solids (TSS): 15.6% Acid content: 1% TSS/acid ratio: 15.6 alternaria: resistant - Seedless - Late ripening

18 Admoni Ripening Season September - October Total soluble solids (TSS): 13.27% Acid content: 1.6% TSS/acid ratio: Early ripening

19 RIPENING SEASON - NOVEMBER TOTAL SOLUBLE SOLIDS (TSS): 13.5% ACID CONTENT: 1.1% TSS/ACID RATIO: 12.3 alternaria: resistant Tami - Seedless - Early ripening --Dwarf Tree

20 Dity RIPENING SEASON - NOVEMBMBER TOTAL SOLUBLE SOLIDS (TSS): 12.8% ACID CONTENT: 1.57% TSS/ACID RATIO: 8.15 alternaria: susceptible - Seedless

21 RIPENING SEASON: DECEMBER-JANUARY TOTAL SOLUBLE SOLIDS (TSS): 17.5% ACID CONTENT: 1.34% TSS/ACID RATIO: 13 alternaria: susceptible Meirav - Seedless -Long Shelf-life

22 Ripening Season: December-January Total soluble solids (tss):15.5% Acid content:1.1% TSS/acid ratio: NECTAR - Seedless -Long Shelf-life

23 RIPENING SEASON: FEBRUARY-MARCH TOTAL SOLUBLE SOLIDS (TSS): 14.27% ACID CONTENT: 1.54% TSS/ACID RATIO: 9.26 alternaria: resistant YIFAT - Seedless --Long Shelf-life

24 RIPENING SEASON: FEBRUARY-MARCH TOTAL SOLUBLE SOLIDS (TSS): 14.1% ACID CONTENT: 2.24% TSS/ACID RATIO: 6.3 alternaria: susceptible WINOLA - Seedless --Long Shelf-life

25 RONIT RIPENING SEASON: FEBRUARY- MARCH TOTAL SOLUBLE SOLIDS (TSS): 18.8% ACID CONTENT: 1.2% TSS/ACID RATIO: alternaria: resistant - Seedless --Long Shelf-life

26 ODEM RIPENING SEASON: JANUARY- FEBRUARY TOTAL SOLUBLE SOLIDS (TSS): 15% ACID CONTENT: 0.96% TSS/ACID RATIO: alternaria: resistant - Seedless --Long Shelf-life

27 RIPENING SEASON: JANUARY-FEBRUARY TOTAL SOLUBLE SOLIDS (TSS): 15.8% ACID CONTENT: 1.2% TSS/ACID RATIO: alternaria: resistant SIGAL

28 DANIT RIPENING SEASON: FEBRUARY-MARCH TOTAL SOLUBLE SOLIDS (TSS): 14.8% ACID CONTENT: 1.28% TSS/ACID RATIO: alternaria: resistant - Seedless --Long Shelf-life

29 MOR- SEEDLESS MURCOTT RIPENING SEASON: JANUARY-MARCH TOTAL SOLUBLE SOLIDS (TSS): 15% ACID CONTENT: 1.3% TSS/ACID RATIO: alternaria: susceptible - Seedless --Long Shelf-life

30 ORRI RIPENING SEASON: JANUARY-APRIL TOTAL SOLUBLE SOLIDS (TSS): 14% ACID CONTENT: 0.7% TSS/ACID RATIO: 20 alternaria: resistant - Seedless --Long Shelf-life

31 FLAMINGO Ripening Season: October-December Total soluble solids (TSS): 12.5% Acid content: 0.52 TSS/acid ratio: 24 - Seedless -High lycopene --Long Shelf-life

32 Aliza RIPENING SEASON: NOVEMBER-FEBRUARY TOTAL SOLUBLE SOLIDS (TSS): 15% ACID CONTENT: 0.64% TSS/ACID RATIO: Seedless --Long Shelf-life

33 EINAT RIPENING SEASON: NOVEMBER-FEBRUARY TOTAL SOLUBLE SOLIDS (TSS): 12.1% ACID CONTENT: 1.1% TSS/ACID RATIO: 11 - Seedless -High lycopene --Long Shelf-life

34 Ayelet-Seedless Villafranca Lemon RIPENING SEASON: ALL YEAR AROUND TOTAL SOLUBLE SOLIDS (TSS):7% ACID CONTENT: 9.6% TSS/ACID RATIO: Seedless --Long Shelf-life

35 Galya-Seedless Urica Lemon RIPENING SEASON: ALL YEAR AROUND TOTAL SOLUBLE SOLIDS (TSS):7% ACID CONTENT:9.8% TSS/ACID RATIO: Seedless --Long Shelf-life

36 1 RIPENING SEASON: JANUARY TOTAL SOLUBLE SOLIDS (TSS):12.4% ACID CONTENT:1.7% TSS/ACID RATIO: Seedless --Long Shelf-life

37 2 RIPENING SEASON: FEBRUARY TOTAL SOLUBLE SOLIDS (TSS):14% ACID CONTENT:1.3% TSS/ACID RATIO: 10.8 alternaria: resistant - Seedless --Long Shelf-life

38 3 RIPENING SEASON: JANUARY TOTAL SOLUBLE SOLIDS (TSS):14.8% ACID CONTENT:1.3% TSS/ACID RATIO: 11.4 alternaria: resistant - Seedless --Long Shelf-life

39 4 RIPENING SEASON: JANUARY TOTAL SOLUBLE SOLIDS (TSS):14.3% ACID CONTENT:1.1% TSS/ACID RATIO: 13 - Low seeds --Long Shelf-life

40 5 RIPENING SEASON: JANUARY TOTAL SOLUBLE SOLIDS (TSS):11.8% ACID CONTENT:1% TSS/ACID RATIO: 11.8 alternaria: resistant - Seedless --Long Shelf-life

41 6 RIPENING SEASON: JANUARY TOTAL SOLUBLE SOLIDS (TSS):11.9% ACID CONTENT:1.1% TSS/ACID RATIO: 10.8 alternaria: resistant - low Seeds --Long Shelf-life

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