LAMPIRAN A SERTIFIKAT PENGUJIAN STANDARISASI EKSTRAK ETANOL DAUN SALAM
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- Kenneth Russell
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1 LAMPIRAN A SERTIFIKAT PENGUJIAN STANDARISASI EKSTRAK ETANOL DAUN SALAM 81
2 LAMPIRAN B SERTIFIKAT PENGUJIAN STANDARISASI EKSTRAK ETANOL HERBA SAMBILOTO 82
3 LAMPIRAN C HASIL UJI PENETAPAN KADAR FLAVONOID TOTAL PADA EKSTRAK ETANOL SALAM-SAMBILOTO Rep. Abs. C obs (µg/ml) FP Csampel (µg/ml) W sampel (gram) Cteoritis (µg/ml) Kadar (%) I 0,593 6, ,807 1, ,167 II 0,651 7, ,843 1, ,182 III 0,502 5, ,338 1, ,143 Keterangan: FP = Faktor pengenceran Perhitungan 4d 0,143* 0,167 0,0075 0,1745 0,182 0, ,0150 : 2 = 0,0075 4d = 4 x 0,0075 = 0,03 0,1745 0,143 > 0,03 0,0315 > 0,03 Data 0,143 di-reject Jadi, rata rata % kadar flavonoid total pada ekstrak adalah 0,175% 83
4 LAMPIRAN D HASIL UJI PENETAPAN KADAR FLAVONOID TOTAL PADA FRAKSI AIR SALAM-SAMBILOTO Rep. Abs. C obs (µg/ml) FP Csampel (µg/ml) W sampel (gram) Cteoritis (µg/ml) Kadar (%) I 0,475 5,453 66, ,526 1, ,908 II 0,485 5,557 66, ,463 1, ,923 III 0,502 5,734 66, ,256 1, ,954 Keterangan: FP = faktor pengenceran Perhitungan 4d 0,908 0,0075 0,9155 0,923 0, ,954* 0,0150 : 2 = 0,0075 4d = 4 x 0,0075 = 0,03 0,954 0,9155 > 0,03 0,0385 > 0,03 Data 0,954 di-reject Jadi, rata rata % kadar flavonoid total pada fraksi air adalah 0,916% 84
5 LAMPIRAN E HASIL UJI MUTU FISIK GRANUL Mutu fisik yang diuji Kelembaban granul Bulk density Tapped density Hausner ratio Carr s index Formula tablet ekstrak etanol salamsambiloto Replikasi F I F II F III F IV I 3,74 3,31 3,23 4,28 II 4,83 4,83 4,33 3,67 4,29 4,07 3,78 3,98 SD 0,77 1,07 0,78 0,43 I 0,38 0,33 0,35 0,33 II 0,33 0,37 0,34 0,33 0,36 0,35 0,35 0,33 SD 0,04 0,03 0,01 0,00 I 0,44 0,39 0,41 0,39 II 0,39 0,43 0,40 0,39 0,42 0,41 0,41 0,39 SD 0,04 0,03 0,01 0,00 I 1,16 1,19 1,16 1,18 II 1,19 1,18 1,18 1,19 1,18 1,19 1,17 1,19 SD 0,02 0,01 0,01 0,01 I 14,00 16,00 14,00 15,50 II 16,00 15,00 15,00 16,00 15,00 15,50 14,50 15,75 SD 1,41 0,71 0,71 0,35 Persyaratan 3-5% (Voigt, 1995) - -,25 (Wells, 1988) 2 (Wells, 1988) 85
6 LAMPIRAN F HASIL UJI KESERAGAMAN BOBOT TABLET EKSTRAK SALAM-SAMBILOTO Hasil uji keseragaman bobot tablet formula I Replikasi I Replikasi II No. Bobot Penyimpangan Bobot Penyimpangan tablet (mg) (%) tablet (mg) (%) 1 703,1 0,22 703,2 0, ,9 0,39 700,7 0, ,1 0,35 703,5 0, ,9 0,18 700,5 0, ,0 0,20 700,8 0, ,6 0,14 701,6 0, ,7 0,56 703,6 0, ,9 0,10 704,2 0, ,6 0,14 701,2 0, ,6 0,42 703,2 0, ,3 0,04 704,7 0, ,5 0,13 700,2 0, ,2 0,06 701,3 0, ,3 0,33 701,8 0, ,9 0,18 705,6 0, ,8 0,26 702,1 0, ,6 0,57 704,3 0, ,7 0,01 706,1 0, ,3 0,19 701,3 0, ,3 0,10 705,2 0,35 704,62 702,76 SD 1,99 1,84 86
7 Hasil uji keseragaman bobot tablet formula II Replikasi I Replikasi II No. Bobot tablet (mg) Penyimpangan (%) Bobot tablet (mg) Penyimpangan (%) 1 701,5 0,33 704,1 0, ,7 0,07 704,5 0, ,4 0,17 701,7 0, ,1 0,16 700,7 0, ,2 0,57 701,3 0, ,5 0,47 699,2 0, ,1 0,01 701,8 0, ,1 0,13 701,5 0, ,1 0,58 704,8 0, ,5 0,24 704,1 0, ,4 0,26 702,4 0, ,4 0,32 702,5 0, ,8 0,06 701,3 0, ,2 0,43 700,5 0, ,4 0,40 702,7 0, ,1 0,13 702,8 0, ,2 0,14 703,5 0, ,8 0,20 704,7 0, ,4 0,03 702,2 0, ,9 0,24 701,4 0,11 699,19 702,39 SD 2,14 1,53 87
8 Hasil uji keseragaman bobot tablet formula III Replikasi I Replikasi II No. Bobot tablet (mg) Penyimpangan (%) Bobot tablet (mg) Penyimpangan (%) 1 709,1 0,39 701,1 0, ,9 0,50 704,1 0, ,9 0,36 708,6 0, ,2 0,87 710,2 0, ,4 0,28 705,4 0, ,6 0,17 707,5 0, ,1 0,32 703,1 0, ,8 0,79 705,3 0, ,6 0,17 705,8 0, ,6 0,46 702,7 0, ,4 0,29 705,2 0, ,8 0,08 700,1 0, ,4 0,28 701,0 0, ,8 0,34 704,7 0, ,2 0,40 703,8 0, ,0 0,34 703,5 0, ,0 0,09 702,5 0, ,0 0,37 707,6 0, ,7 0,24 704,5 0, ,9 0,35 707,1 0,34 706,37 704,69 SD 2,92 2,63 88
9 Hasil uji keseragaman bobot tablet formula IV Replikasi I Replikasi II No. Bobot Penyimpangan Bobot Penyimpangan tablet (mg) (%) tablet (mg) (%) 1 705,7 0,63 708,0 0, ,5 0,82 707,3 0, ,4 0,73 708,5 0, ,9 0,37 707,5 0, ,1 0,45 708,2 0, ,9 0,80 709,8 0, ,9 0,09 708,2 0, ,3 0,56 706,7 0, ,3 0,28 709,2 0, ,9 0,20 705,1 0, ,6 0,24 708,7 0, ,3 0,43 708,7 0, ,7 0,51 707,4 0, ,4 0,27 707,4 0, ,7 0,92 707,9 0, ,3 0,14 708,9 0, ,2 0,42 709,0 0, ,3 0,85 703,4 0, ,2 0,42 709,5 0, ,0 0,47 709,7 0,27 701,28 707,76 SD 3,86 1,85 89
10 LAMPIRAN G HASIL UJI KEKERASAN TABLET EKSTRAK ETANOL SALAM- SAMBILOTO No. Kekerasan tablet ekstrak etanol salam-sambiloto (kp) F I F II F III F IV Rep I Rep II Rep I Rep II Rep I Rep II Rep I Rep II 1 5,70 5,20 5,70 5,90 7,00 5,80 7,60 7,60 2 6,10 6,00 6,60 6,10 5,60 5,70 7,70 7,50 3 6,30 5,50 6,20 6,30 6,50 6,30 6,90 7,40 4 5,50 5,60 6,50 6,30 5,40 6,30 7,10 7,00 5 5,10 5,80 6,40 6,20 6,30 6,10 8,00 6,90 6 5,70 5,60 6,10 6,10 6,60 6,10 7,50 6,70 7 6,30 5,70 6,00 5,90 6,40 6,30 6,80 7,40 8 5,90 5,70 6,30 6,40 6,30 6,00 6,80 7,50 9 5,40 5,50 6,20 5,90 6,70 5,90 6,60 7, ,00 5,80 6,20 5,80 6,50 5,70 6,90 7,20 5,80 5,64 6,22 6,09 6,33 6,02 7,19 7,23 SD 0,37 0,22 0,26 0,21 0,49 0,24 0,47 0,30 90
11 LAMPIRAN H HASIL UJI KERAPUHAN TABLET EKSTRAK ETANOL SALAM- SAMBILOTO Formula Rep Berat awal (gram) Berat akhir (gram) Kerapuhan (%) Rata - rata SD KV (%) I II III IV I 14,14 14,08 0,46 II 14,05 13,99 0,45 I 14,00 13,95 0,36 II 14,08 14,02 0,43 I 14,14 14,08 0,45 II 14,06 14,00 0,44 I 14,04 13,98 0,39 II 14,20 14,15 0,39 0,46 0,00 0,96 0,39 0,05 12,47 0,44 0,00 0,86 0,39 0,00 0,85 91
12 LAMPIRAN I HASIL UJI WAKTU HANCUR TABLET EKSTRAK ETANOL SALAM-SAMBILOTO Formula Waktu hancur tablet ekstrak etanol salam-sambiloto (menit) Rata - rata Replikasi I Replikasi II SD KV (%) I 39,63 42,42 41,03 1,97 4,81 II 48,75 48,95 48,85 0,14 0,29 III 40,37 38,36 39,37 1,42 3,61 IV 43,33 42,20 42,77 0,08 1,87 92
13 LAMPIRAN J CONTOH PERHITUNGAN Contoh perhitungan Carr s index dan Hausner ratio: Formula I: Berat gelas ukur kosong (W 1 ) = 105,6253 gram Berat gelas ukur kosong + granul (W 2 ) = 143,7159 gram V 1 V 2 = 100 ml = 86 ml Bulk density = = 0,3809 Tapped density = = 0,4429 Carr s index = x 100% = x 100% = 14,00% Hausner ratio = = = 1,16 Contoh Perhitungan Kadar Flavonoid Total Fraksi Air dan Ekstrak Etanol Salam-Sambiloto Ekstrak etanol salam-sambiloto Rep. Abs. C obs (µg/ml) FP Csampel (µg/ml) W sampel (mg) Cteoritis (µg/ml) Kadar (%) I 0,593 6, ,807 1, ,167 93
14 perhitungan : Abs = 0,593 y= -0, ,0961x C obs (x) = 6,681 µg/ml Csampel = C obs x FP = 6,681 x 10 = 66,807 µg/ml Cteoritis = = = µg/ml Kadar (%) = = x 100% = 0,167% Contoh Perhitungan Kadar Flavonoid Total dalam Tablet Ekstrak Etanol Salam-Sambiloto Rep. Abs. C obs (µg/ml) FP Csampel (µg/ml) W sampel (mg) Kadar total flavonoid (%) Bobot rata rata tablet (mg) Perolehan kembali (%) I 0,231 2, , ,6 0, ,58 Perolehan kembali = x 100 % Perhitungan : Abs = 0,231 y= -0, ,0961x C obs (x) = 2,914 µg/ml Csampel = C obs x FP = 2,914 x 10 = 29,139 µg/ml Perolehan kembali = x 100 % = 92,58% 94
15 LAMPIRAN K TABEL UJI r 95
16 LAMPIRAN L TABEL UJI F 96
17 97
18 LAMPIRAN M HASIL UJI STATISTIK KESERAGAMAN BOBOT TABLET ANTAR FORMULA N Mean Std. Deviation Descriptives Std. Error 95% Confidence Interval for Mean Lower Bound Upper Bound Minimum Maximum form , , , , , , ,620 form , , , , , , ,390 form , , , , , , ,370 form , , , , , , ,760 Total 8 703, , , , , , ,760 ANOVA: One Way Sum of squares df Mean square F Sig. Between groups 24, ,314 1,137 0,435 Within groups 29, ,314 total 54,199 7 Multiple comparisons Dependent variable: keseragaman bobot Tukey HSD Mean 95% confidence interval (I) (J) difference Std. Error Sig. formula formula Lower Upper (I-J) bound bound form 1 form 2 2, , ,723-8, ,90943 form 3-1, , ,900-12, ,16943 form 4-0, , ,989-11, ,17943 form 2 form 1-2, , ,723-13, ,10943 form 3-4, , ,407-15, ,26943 form 4-3, , ,570-14, ,27943 form 3 form 1 1, , ,900-9, ,84943 form 2 4, , ,407-6, ,74943 form 4 1, , ,980-9, ,01943 form 4 form 1 0, , ,989-10, ,83943 form 2 3, , ,570-7, ,73943 form 3-1, , ,980-12, ,
19 LAMPIRAN N HASIL UJI STATISTIK KEKERASAN TABLET ANTAR FORMULA N Mean Std. Deviation Std. Error Descriptive 95% Confidence Interval for Mean Lower Bound Upper Bound Minimum Maximum form 1 2 5,7200 0, , ,7035 6,7365 5,64 5,80 form 2 2 6,1550 0, , ,3291 6,9809 6,09 6,22 form 3 2 6,1750 0, , ,2055 8,1445 6,02 6,33 form 4 2 7,2100 0, , ,9559 7,4641 7,19 7,23 Total 8 6,3150 0, , ,8183 6,8117 5,64 7,23 ANOVA: One Way Sum of squares df Mean square F Sig. Between groups 2, ,800 45,659 0,001 Within groups 0, ,018 total 2,471 7 Dependent variable: kekerasan Tukey HSD (I) formula (J) formula Mean difference (I-J) Multiple comparisons Std. Error Sig. 95% confidence interval Lower Upper bound bound form 1 form 2-0, , ,096-0,9739 0,1039 form 3-0, , ,084-0,9939 0,0839 form 4-1,49000 * 0, ,001-2,0289-0,9511 form 2 form 1 0, , ,096-0,1039 0,9739 form 3-0, , ,999-0,5589 0,5189 form 4-1,05500 * 0, ,005-1,5939-0,5161 form 3 form 1 0, , ,084-0,0839 0,9939 form 2 0, , ,999-0,5189 0,5589 form 4-1,03500 * 0, ,005-1,5739-0,4961 form 4 form 1 1,49000 * 0, ,001 -,9511 2,0289 form 2 1,05500 * 0, ,005 0,5161 1,5939 form 3 1,03500 * 0, ,005 0,4961 1,5739 *. The mean difference is significant at the 0,05 level. 99
20 LAMPIRAN O HASIL UJI STATISTIK KERAPUHAN TABLET ANTAR FORMULA N Mean Std. Deviatio n Std. Error Descriptives 95% Confidence Interval for Mean Lower Bound Upper Bound Minimum Maximum form 1 2 0,4550 0, , ,3915 0,5185 0,45 0,46 form 2 2 0,3950 0, , ,0497 0,8397 0,36 0,43 form 3 2 0,4450 0, , ,3815 0,5085 0,44 0,45 form 4 2 0,3900 0, , ,3900-0,3900 0,39 0,39 Total 8 0,4213 0, , ,3908 0,4517 0,36 0,46 ANOVA: One Way Sum of squares df Mean square F Sig. Between groups 0, ,002 3,523 0,128 Within groups 0, ,001 total 0,009 7 Dependent variable: kerapuhan Tukey HSD Multiple comparisons Mean 95% confidence interval (I) (J) difference Std. Error Sig. formula formula Lower Upper (I-J) bound bound form 1 form 2 0, , ,224-0,0428 0,1628 form 3 0, , ,976-0,0928 0,1128 form 4 0, , ,185-0,0378 0,1678 form 2 form 1-0, , ,224-0,1628 0,0428 form 3-0, , ,328-0,1528 0,0528 form 4 0, , ,997-0,0978 0,1078 form 3 form 1-0, , ,976-0,1128 0,0928 form 2 0, , ,328-0,0528 0,1528 form 4 0, , ,271-0,0478 0,1578 form 4 form 1-0, , ,185-0,1678 0,0378 form 2-0, , ,997-0,1078 0,0978 form 3-0, , ,271-0,1578 0,
21 LAMPIRAN P HASIL UJI STATISTIK WAKTU HANCUR TABLET ANTAR FORMULA N Mean Std. Deviation Descriptives Std. Error 95% Confidence Interval for Mean Lower Bound Upper Bound Minimum Maximum form ,0250 1, , , , ,63 42,42 form ,8500 0, , , , ,75 48,95 form ,3650 1, , , , ,36 40,37 form ,8750 0, , , , ,42 43,33 Total 8 43,0288 3, , , , ,36 48,95 ANOVA: One Way Sum of squares df Mean square F Sig. Between groups 102, ,261 20,857 0,007 Within groups 6, ,643 total 109,354 7 Multiple comparisons Dependent variable: waktu hancur Tukey HSD Mean 95% confidence interval (I) (J) Std. difference Sig. formula formula Error Lower Upper (I-J) bound bound form 1 form 2-7,82500 * 1, ,012-13,0424-2,6076 form 3 1, , ,611-3,5574 6,8774 form 4-1, , ,580-6,9574 3,4774 form 2 form 1 7,82500 * 1, ,012 2, ,0424 form 3 9,48500 * 1, ,006 4, ,7024 form 4 6,08500 * 1, ,030 0, ,3024 form 3 form 1-1, , ,611-6,8774 3,5574 form 2-9,48500 * 1, ,006-14,7024-4,2676 form 4-3, , ,172-8,6174 1,8174 form 4 form 1 1, , ,580-3,4774 6,9574 form 2-6,08500 * 1, ,030-11,3024-0,8676 form 3 3, , ,172-1,8174 8,6174 *. The mean difference is significant at the 0,05 level. 101
22 LAMPIRAN Q HASIL UJI ANAVA KEKERASAN TABLET EKSTRAK ETANOL SALAM-SAMBILOTO DENGAN DESIGN EXPERT Response 1 kekerasan ANOVA for selected factorial model Analysis of variance table [Partial sum of squares - Type III] source Sum of Mean p-value df F value squares square Prob > F Model 2,40 3 0,80 45,66 0,0015 significant A-PVP K-30 1,08 1 1,08 61,65 0,0014 B-Crospovidone 1,14 1 1,14 65,05 0,0013 AB 0,18 1 0,18 10,27 0,0327 Pure error 0, ,018 Cor total 2,47 7 The Model F-value of implies the model is significant. There is only a 0.15% chance that a "Model F-Value" this large could occur due to noise. Values of "Prob > F" less than indicate model terms are significant. In this case A, B, AB are significant model terms. Values greater than indicate the model terms are not significant. If there are many insignificant model terms (not counting those required to support hierarchy), model reduction may improve your model. Std. Dev R-Squared Mean 6.31 Adj R-Squared C.V. % 2.10 Pred R-Squared PRESS 0.28 Adeq Precision The "Pred R-Squared" of is in reasonable agreement with the "Adj R-Squared" of
23 "Adeq Precision" measures the signal to noise ratio. A ratio greater than 4 is desirable. Your ratio of indicates an adequate signal. This model can be used to navigate the design space. Factor Coefficient estimate df Standard error 95% CI low 95% CI high VIF Intercept 6,31 1 0,047 6,19 6,44 A-PVP K-30 0,37 1 0,047 0,24 0,50 1,00 B-Crospovidone 0,38 1 0,047 0,25 0,51 1,00 AB 0,15 1 0,047 0,020 0,28 1,00 Final Equation in Terms of Coded Factors: kekerasan = +6,31 +0,37 *A +0,38 *B +0,15 *A*B Final Equation in Terms of Actual Factors: kekerasan = +6, ,36750 * PVP K-30 +0,37750 * Crospovidone +0,15000 * PVP K-30 * Crospovidone The Diagnostics Case Statistics Report has been moved to the Diagnostics Node. In the Diagnostics Node, Select Case Statistics from the View Menu. 103
24 Proceed to Diagnostic Plots (the next icon in progression). Be sure to look at the: 1) Normal probability plot of the studentized residuals to check for normality of residuals. 2) Studentized residuals versus predicted values to check for constant error. 3) Externally Studentized Residuals to look for outliers, i.e., influential values. 4) Box-Cox plot for power transformations. If all the model statistics and diagnostic plots are OK, finish up with the Model Graphs icon. 104
25 LAMPIRAN R HASIL UJI ANAVA KERAPUHAN TABLET EKSTRAK ETANOL SALAM-SAMBILOTO DENGAN DESIGN EXPERT Response 2 kerapuhan ANOVA for selected factorial model Analysis of variance table [Partial sum of squares - Type III] source Sum of squares df Mean square F value p-value Prob > F Model 6,738E ,246E-003 3,52 0,1277 A-PVP K-30 6,613E ,613E ,37 0,0323 B-Crospovidone 1,125E ,125E-004 0,18 0,6960 AB 1,250E ,250E-005 0,020 0,8954 Pure error 2,550E ,375E-004 Cor total 9,288E not significant The "Model F-value" of 3.52 implies the model is not significant relative to the noise. There is a % chance that a "Model F-value" this large could occur due to noise. Values of "Prob > F" less than indicate model terms are significant. In this case A are significant model terms. Values greater than indicate the model terms are not significant. If there are many insignificant model terms (not counting those required to support hierarchy), model reduction may improve your model. Std. Dev R-Squared Mean 0.42 Adj R-Squared C.V. % 5.99 Pred R-Squared PRESS Adeq Precision
26 A negative "Pred R-Squared" implies that the overall mean is a better predictor of your response than the current model. "Adeq Precision" measures the signal to noise ratio. A ratio of 3.64 indicates an inadequate signal and we should not use this model to navigate the design space. Factor Coefficient Standard 95% CI 95% CI df estimate error low high VIF Intercept 0,42 1 8,927E-003 0,40 0,45 A-PVP K-30-0, ,927E-003-0,054-3,965E ,00 B-Crospovidone -3,750E ,927E-003-0,029 0,021 1,00 AB 1,250E ,927E-003-0,024 0,026 1,00 Final Equation in Terms of Coded Factors: kerapuhan = +0,42-0,029 *A -3,750E-003 *B +1,250E-003 *A*B Final Equation in Terms of Actual Factors: kerapuhan = +0, , *PVP K-30-3,75000E-003 *Crospovidone +1,25000E-003 *PVP K-30*Crospovidone The Diagnostics Case Statistics Report has been moved to the Diagnostics Node. In the Diagnostics Node, Select Case Statistics from the View Menu. 106
27 Proceed to Diagnostic Plots (the next icon in progression). Be sure to look at the: 1) Normal probability plot of the studentized residuals to check for normality of residuals. 2) Studentized residuals versus predicted values to check for constant error. 3) Externally Studentized Residuals to look for outliers, i.e., influential values. 4) Box-Cox plot for power transformations. If all the model statistics and diagnostic plots are OK, finish up with the Model Graphs icon. 107
28 LAMPIRAN S HASIL UJI ANAVA WAKTU HANCUR TABLET EKSTRAK ETANOL SALAM-SAMBILOTO DENGAN DESIGN EXPERT Response 3 waktu hancur ANOVA for selected factorial model Analysis of variance table [Partial sum of squares - Type III] source Sum of Mean p-value df F value squares square Prob > F Model 102, ,26 20,86 0,0066 significant A-PVP K-30 63, ,00 38,35 0,0035 B-Crospovidone 29, ,99 18,26 0,0129 AB 9,79 1 9,79 5,96 0,0711 Pure error 6,57 4 1,64 Cor total 109,35 7 The Model F-value of implies the model is significant. There is only a 0.66% chance that a "Model F-Value" this large could occur due to noise. Values of "Prob > F" less than indicate model terms are significant. In this case A, B are significant model terms. Values greater than indicate the model terms are not significant. If there are many insignificant model terms (not counting those required to support hierarchy), model reduction may improve your model. Std. Dev R-Squared Mean Adj R-Squared C.V. % 2.98 Pred R-Squared PRESS Adeq Precision The "Pred R-Squared" of is in reasonable agreement with the "Adj R-Squared" of
29 "Adeq Precision" measures the signal to noise ratio. A ratio greater than 4 is desirable. Your ratio of indicates an adequate signal. This model can be used to navigate the design space. Factor Coefficient Standard 95% CI 95% CI df estimate error low high VIF Intercept 43,00 1 0,45 41,74 44,26 A-PVP K-30 2,81 1 0,45 1,55 4,06 1,00 B-Crospovidone -1,94 1 0,45-3,19-0,68 1,00 AB -1,11 1 0,45-2,36 0,15 1,00 Final Equation in Terms of Coded Factors: waktu hancur = +43,00 +2,81 *A -1,94 *B -1,11 *A*B Final Equation in Terms of Actual Factors: waktu hancur = +43, ,80625 * PVP K-30-1,93625 * Crospovidone -1,10625 * PVP K-30 * Crospovidone The Diagnostics Case Statistics Report has been moved to the Diagnostics Node. In the Diagnostics Node, Select Case Statistics from the View Menu. 109
30 Proceed to Diagnostic Plots (the next icon in progression). Be sure to look at the: 1) Normal probability plot of the studentized residuals to check for normality of residuals. 2) Studentized residuals versus predicted values to check for constant error. 3) Externally Studentized Residuals to look for outliers, i.e., influential values. 4) Box-Cox plot for power transformations. If all the model statistics and diagnostic plots are OK, finish up with the Model Graphs icon. 110
31 LAMPIRAN T HASIL UJI MUTU FISIK TABLET EKSTRAK SALAM- SAMBILOTO BERDASARKAN FORMULA OPTIMUM Keseragaman bobot No. Bobot tablet (mg) Replikasi I Penyimpangan (%) Bobot tablet (mg) Replikasi II Penyimpangan (%) 1 701,2 0,49 702,5 0, ,3 1,04 699,4 0, ,2 0,06 712,9 1, ,3 0,66 691,2 1, ,0 0,76 692,0 1, ,4 0,82 707,0 0, ,0 0,80 706,0 0, ,2 0,35 704,3 0, ,5 0,16 702,8 0, ,1 0,65 704,5 0, ,1 0,79 703,8 0, ,2 0,36 717,4 2, ,4 0,25 707,4 0, ,5 0,45 705,4 0, ,5 0,55 699,0 0, ,1 0,65 699,8 0, ,8 0,31 700,8 0, ,9 1,03 701,8 0, ,9 0,60 702,4 0, ,4 0,11 705,4 0,30 704,65 703,29 SD 4,42 5,94 111
32 Kekerasan tablet Kekerasan tablet ekstrak etanol salamsambiloto No. (kp) Replikasi I Replikasi II 1 6,00 6,30 2 5,50 6,90 3 5,80 5,80 4 5,40 6,50 5 5,90 6,00 6 6,20 6,30 7 6,00 5,90 8 6,20 6,20 9 5,80 6, ,70 6,50 5,85 6,27 SD 0,27 0,32 Kerapuhan tablet Replikasi Berat awal (gram) Berat akhir (gram) Kerapuhan (%) I 14,37 14,30 0,47 II 14,48 14,42 0,46 Rata - rata SD KV (%) 0,46 0,00 0,37 Waktu hancur tablet Replikasi Waktu hancur tablet ekstrak etanol salamsambiloto (menit) I 40,05 II 39,72 Rata - rata SD KV (%) 39,89 0,23 0,59 112
33 LAMPIRAN U TABEL UJI T 113
34 LAMPIRAN V HASIL UJI STATISTIK HASIL PERCOBAAN DAN HASIL TEORITIS PADA KEKERASAN TABLET EKSTRAK ETANOL SALAM-SAMBILOTO One-Sample Statistics N Mean Std. Deviation Std. Error Mean kekerasan 2 6,0600 0, ,21000 t df One-Sample Test Sig. (2- tailed) Test Value = 6.17 Mean Difference 95% Confidence Interval of the Difference Lower Upper kekerasan -0, ,693-0, ,7783 2,
35 LAMPIRAN W HASIL UJI STATISTIK HASIL PERCOBAAN DAN HASIL TEORITIS PADA KERAPUHAN TABLET EKSTRAK ETANOL SALAM-SAMBILOTO One-Sample Statistics N Mean Std. Deviation Std. Error Mean kerapuhan 2 0,4650 0, ,00500 t df Sig. (2- tailed) One-Sample Test Test Value = 0.44 Mean Difference 95% Confidence Interval of the Difference Lower Upper kerapuhan 5, ,126 0, ,0385 0,
36 LAMPIRAN X HASIL UJI STATISTIK HASIL PERCOBAAN DAN HASIL TEORITIS PADA WAKTU HANCUR TABLET EKSTRAK ETANOL SALAM-SAMBILOTO One-Sample Statistics N Mean Std. Deviation Std. Error Mean waktuhancur 2 39,8850 0, ,16500 One-Sample Test Test Value = t df Sig. (2- tailed) Mean Difference 95% Confidence Interval of the Difference Lower Upper waktuhancur 1, ,344 0, ,8215 2,
37 LAMPIRAN Y HASIL PENETAPAN KADAR FLAVONOID TOTAL TABLET FORMULA OPTIMUM Rep. Abs. C obs (µg/ml) FP Csampel (µg/ml) W sampel (mg) Kadar flavonoid total (%) Bobot rata rata tablet (mg) Perolehan kembali (%) I 0,231 2, , ,6 0, ,58 II 0,234 2, , ,1 0, ,47 117
38 LAMPIRAN Z SERTIFIKAT ANALISIS BAHAN Ekstrak Etanol Salam 118
39 Ekstrak Etanol Sambiloto 119
40 Crospovidone 120
41 121
42 122
43 PVP K
44 124
45 Laktosa Monohidrat 125
46 126
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