Allergen Lateral Flow IIR

Transcription

1 Allergen Lateral Flow IIR Products Egg (Ovalbumin) Lateral Flow IIR Casein Lateral Flow IIR Gluten (Gliadin) Lateral Flow IIR Peanut Lateral Flow IIR High sensitivity, Fast and Easy Especially, superior to test for processed food.

2 Intended Use Unprocessed and processed foods Lateral Flow IIR determines quickly whether or not target proteins reside in foods, including unprocessed foodstuffs and processed products. The novel extraction solution provided with the kit enhances the extraction recovery yield of target proteins from test samples, even from extensively processed foods, leading to more reliable results than ever. For the best results, please follow the instructions exactly. Swabbing test Lateral Flow IIR is also applicable to swab tests for the contamination on surfaces of food-processing equipments. Performance Performance Sample preparation time Chromatographic run time Detection limit for food Detection limit for swabbing test 30min 15min 5 ppm (μg protein /g food) 250ng/mL in Sample Extract Detection limit is shown by each protein concentration as follows, Egg : whole egg protein, Casein : whole milk protein, Gluten : whole wheat protein, Peanut : whole peanut protein High light Innovative extraction method Using an innovative extraction solution, the recovery of allergen protein is significantly increased. Special antibodies Our antibodies can detect the target allergen protein even after the exposure to extreme processing condition. Accordingly, the kits can apply for the examination of highly processed foods, as well as swab samples collected in various point of food processing.

3 Validation data of Lateral flow IIR Sensitivity concentration Egg Casein Gluten Peanut 200 ppm ppm ppm ppm Egg : ppm (μg egg protein /g food), Casein : ppm (μg milk protein /g food) Gluten : ppm (μg wheat protein /g food), Peanut : ppm (μg peanut protein /g food) Representative test result of commercial foods Food Egg Chocolate biscuit + Bread + Instant noodle + Mayonnaise + Food Casein Chocolate biscuit + Bread + Snack + Margarine + Food Gluten Chocolate biscuit + Snack + Food Peanut Peanut chocolate + Peanut butter + Peanut cream +

4 Reactivity of model processed foods Model processed foods were prepared by spiking egg, milk, wheat, and peanut powder at 5 ppm (μg protein /g food) to the food before processing. Egg : ppm (μg egg protein /g food), Milk : ppm (μg milk protein /g food) Gluten : ppm (μg wheat protein /g food), Peanut : ppm (μg peanut protein /g food) Add egg, milk, wheat, peanut powder Raw materials Food processing (heating) Model processed foods ( 5 ppm) Test Food Heating condition Egg Casein Gluten Peanut Orange juice Heated at 90 for 10min Jerry Heated up to reach Porridge Cooked by a rice cooker Baby food 1 (retort) Baby food 2 (retort) Strawberry jam Bread Heated at 120 for 20min Heated at 120 for 20min Boiled until it has evaporated Approximately, Heated at for 40min (Cooked bread baking machine) Not test +

5 Cross reactivity of Lateral Flow IIR Food Egg Casein Gluten Peanut Egg Milk Skim milk Wheat *1 - Barley *2 - Rye *2 - Oats Soy bean Corn flour Peanut Almond (Roasted) Cashew (Roasted) Macadamia (Roasted) Pistachio (Roasted) Walnut (Roasted) Sesame Black pepper Red pepper Cumin Coriander Poppy seed Shrimp Crab Squid Beef Pork Chicken This data is representative. Please contact us when you need lot data you use. *1 : This sample was diluted 20-fold and analyzed. *2 : These samples were diluted 5-fold and analyzed.

6 Picture of Lateral Flow IIR Positive Negative

7 Related Food Allergen Test Kits Food Allergen ELISA Kit Product Assay Range Limit of Detection Egg (Ovalbumin) ELISA Kit ppm 0.31 ppm Casein ELISA Kit ppm 0.31 ppm Gluten (Gliadin) ELISA Kit ppm 0.31 ppm Peanut ELISA Kit ppm 0.31 ppm Egg : ppm (μg egg protein /g food), Casein : ppm (μg milk protein /g food) Gluten : ppm (μg wheat protein /g food), Peanut : ppm (μg peanut protein /g food) Contact For further information, please contact info@miobs.com

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