SUPPLEMENTARY INFORMATION. Supplemental Figure 1. Body weight and blood glucose parameters of chow-diet (CD)
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1 SUPPLEMENTARY INFORMATION LEGENDS Supplemental Figure. Body weight and blood glucose parameters of chow-diet (CD) fed and high-fat diet (HFD) fed mice. (A) Body weight was measured at the beginning of diet (day ) and at termination ( months) for both CD-fed and HFD-fed mice. (B) Blood glucose concentration was measured at the beginning of diet (day ) and at the termination ( months) for both CD-fed and HFD-fed mice. (C) Intra-peritoneal glucose tolerance test (ipgtt) was performed at 7 weeks of diet from both CD-fed and HFD-fed mice. Time course measurement of blood glucose concentration was performed following an intra-peritoneal injection of glucose ( g/kg) after 6h of fasting. (D) Iulin tolerance test (ITT) was performed at 7 weeks of diet on both CD-fed and HFD-fed mice. Time course measurement of blood glucose concentration was performed following an intra-peritoneal injection of iulin (.7 IU/kg) after 6h of fasting. Results are plotted as whiskers box (whiskers: minimum to maximum values). p <., p <.. Supplemental Figure. Cell-sorting from SVF from ob/ob mice ewat by FACS. Cells were stained using appropriated antibodies (see Material and Methods) then sorted by FACS. Gating was performed according to the antibody signal inteity for endothelial cells (CD- /CD+, orange), preadipocytes/amscs (CD-/CD-, pink) and macrophages (F/8+, blue). For lymphocytes, gating was performed according both cell size/structure and CD+ signal inteity (green). Results are displayed as coloured isolated cell-type according to gating from signal inteity or size/structure. Supplemental Figure. Expression of markers of adipocytes and preadipocytes/amscs from ob/ob mice ewat. Expression of adipocyte markers (Adiponectin, PPARg and Fabp) and preadipocyte/amsc markers (CD and Sca-) was monitored by qpcr in both
2 adipocyte fraction from ewat from ob/ob mice and preadipocyte/amsc sorted from SVF from ewat from ob/ob mice. Results for (A) Adiponectin, (B) PPARγ and (C) Fabp are displayed as expression relatively to preadipocytes/amscs. Results of expression of (D) CD and (E) Sca- are displayed as fold expression compared to adipocytes. p <., p <.. Supplemental Figure. Expression of markers of macrophages, lymphocytes, haematopoietic cells and endothelial cells from SVF from ob/ob mice ewat. Expression levels of macrophage marker (F/8), lymphocyte marker (CDe), haematopoietic cell marker (CD) and endothelial cell marker (CD) were quantified by qpcr in preadipocytes/amscs (dark blue), endothelial cells (red), macrophages (light blue), lymphocytes (green) sorted from SVF of ob/ob mice ewat. Results for (A) F/8, (B) CDe, (C) CD and (D) CD are displayed as fold-expression compared to non-fractionated SVF. p <., p <.. Supplemental Figure. Cell-sorting from SVF from both CD-fed and HFD-fed mice ewat by FACS. (A) Body weight was measured at the beginning of diet (day ) and at the termination ( months) from both CD-fed and HFD-fed mice. p <. (B) Dendogram depicting the preadipocytes SVF-sorting strategy. (C) Gating strategy for preadipocyte sorting and isolation from ewat from both CD-fed and HFD-fed mice. Preadipocytes were identified as CD - CD - Sca- + CD9 + CD + CD - cells. Supplemental Figure 6. Expression of identified mirna in preadipocytes sorted from SVF from CD-fed and HFD-fed mice ewat. Expression levels of (A) mir-a, (B) mir- b, (C) mir--p, (D) mir--p and (E) mir--p were quantified by qpcr in preadipocytes isolated from SVF from CD-fed and HFD-fed mice ewat. Results are
3 displayed as expression of each mirna in preadipocytes from HFD-fed mice ewat relative to that in preadipocytes from CD-fed mice ewat. n indicates the number of mice used for each experiment. p <.. Supplemental Figure 7. Expression level of mir-a and mir-b in subcutaneous WAT of lean, normoglycemic, glucose intolerant and diabetic patients. Expression level of (A) mir-a and (B) mir-b was quantified by qpcr in the subcutaneous WAT of lean, normoglycemic, glucose intolerant and diabetic patients. Results are displayed as the expression of each mirna in tissue from obese patients relative to that in control lean patients. Results are plotted as whiskers box (whiskers: minimum to maximum values). Supplemental Figure 8. Expression level of VTmR related to each identified mirna in endothelial cells, macrophages and lymphocytes from SVF from ob/ob mice ewat. Deregulated VTmRs related to mir-a (Flt, Gata6, Vcam, Zeb and Zfpm), mir-b (Flt, Ptpn, Zeb and Zfpm), mir--p (Bmp7 and Dnmt) and mir--p (Lrg, Mapk, Rasa and Tnc) in non-fractionated ewat were selected for subsequent expression level analysis in the distinct cell subtypes sorted from SVF from ob/ob mice ewat. Results are displayed as expression of each VTmR in endothelial cells, macrophages and lymphocytes relatively to preadipocytes/amscs from SVF from ob/ob mice ewat. Supplemental Figure 9. Expression of selected VTmRs in preadipocytes sorted from SVF from CD-fed and HFD-fed mice ewat. Expression levels of (A) Flt, (B) Vcam and (C) Bmp7 were quantified by qpcr in preadipocytes isolated from SVF from CD-fed and HFD-fed mice ewat. Results are displayed as expression of each gene in preadipocytes from HFD-fed mice ewat relative to that in preadipocytes from CD-fed mice ewat. n indicates the number of mice used for each experiment. p <..
4 A Oger F. et al., Figure SI Body weight (g) Day months Day months chow diet high fat diet Blood glucose (mg/dl) Day months Day months chow diet high fat diet 8 ipgtt Blood glucose (mg/dl) 6 high fat diet chow diet 6 9 Time (min) ITT Blood glucose (mg/dl) high fat diet chow diet 6 9 Time (min)
5 Oger F. et al., Figure SI
6 A. F/8 Oger F. et al., Figure SI.. SVF preadipocytes /AMSCs endothelial cells macrophages lymphocytes CDe SVF preadipocytes /AMSCs endothelial cells macrophages lymphocytes. CD.. SVF preadipocytes /AMSCs endothelial cells macrophages lymphocytes CD SVF preadipocytes /AMSCs endothelial cells macrophages lymphocytes
7 Oger F. et al., Figure SI A CD B Sca Adipocytes Preadipocytes/AMSCs Adipocytes Preadipocytes/AMSCs Adiponectin D Pparγ Preadipocytes/AMSCs Adipocytes Preadipocytes/AMSCs Adipocytes Fabp Preadipocytes/AMSCs Adipocytes
8 Oger F. et al., Figure SI A 8 B ewat SVF Adipocytes Body weight (g) 6 p=.68 CD- CD+ Sca-- Sca-+ Day months Day months chow diet high fat diet CD+ CD- CD9- CD9+ CD- CD+ mirna expression gene expression Preadipocytes sorted from HFD-fed mice Preadipocytes sorted from CD-fed mice
9 Oger F. et al., Figure SI6 A mir-a B mir-b. p=.7... CD HFD CD HFD n= n= n= n= mir--p D mir--p.. CD HFD CD HFD n= n= n= n= mir--p.. CD HFD n= n=
10 obese patients Oger F. et al., Figure SI7 diabetic obese patients A mir-a B mir-b control normoglycemic glucose intolerant diabetic control normoglycemic glucose intolerant
11 Flt Vcam Oger F. et al., Figure SI8 Zfpm Ptpn Bmp7 Dnmt Tnc Mapk Rasa Lrg 8 Gata6 Zeb 6 6
12 Oger F. et al., Figure SI9 A Vcam.. CD HFD n= n= Bmp7.. CD HFD n= n=
13 Primer sequences ( ) Genes Genebank accession number ACTGTCAAGCTAGAGGTGTCC Flt NM_8 ACCTATAGACACCCTCATCCTC GTGCCTCGACCACTTGCTAT Gata6 NM_8 ACAGAGCCACTGCTGTTACC AACCGAATCCCCAACTTGTGCAG Vcam NM_69 TCTCCAGCTTCTCTCAGGAAATGCC GCATGTGACCTGTGTGACAA Zeb NM_6 CACACTCGTGAGGCCTCTTA AATGCACCAAGAGCTTTTCC Zfpm NM_766 GGGCAGGAACTCTTCCATTT CACAATGGGGAGGACAACTT Ptpn NM_ CCAGTGGCTGTGGGATAAAT AGCGATTTGACAACGAGACC Bmp7 NM_77 GCTGTCCAGCAAGAAGAGGT CCTAGTTCCGTGGCTACGAGGAGAA Dnmt NM_99 TCTCTCTCCTCTGCAGCCGACTCA AGTGGGAGCTTCCAGGAACA Tnc NM_67 TGGTGTCCAGACGACCTTCTG CACGTTGGTACAGAGCTCCA Mapk NM_99 TGTGATTCAGCTGGTCAAGG TGTCCATGACAGTCTCTTCG Rasa NM_ GGAGTTTCTCCTGCAAAGTAAAA AGAATTCCACCGACAGATGG Lrg NM_9796 GCCTCAAGGAATGCCTGATA CATGCTCAACATCTCCCCCTTCTCC Rplp (6B) NM_ GGGAAGGTGTAATCCGTCTCCACAG GTGCAGAAAGAGCTCAGGGAC Sca- NM_76 TCCATCAGGGTAGGGGCAGG TAAGACCACACCAGCCATCTC CD NM_6 TGGAGTTCCAGAGCCTGAAG CCGTGATGGAAGACCACTCG Pparγ NM_6 AGGCCTGTTGTAGAGCTGGGT C GTGATGCCTTTGTGGGAACCTGG Fabp NM_6 TGTGGCAAAGCCCACTCCCACT GTGATGGCAGAGATGGCACT Adipoq (Adiponectin) NM_96 GCTTCTCCAGGCTCTCCTTT CTTTGGCTATGGGCTTCCAGTC F/8 NM_ GCAAGGAGGACAGAGTTTATCGTG
14 CTGCTACACACCAGCCTCAA CAGGTCCACCTCCACACAGT AGGCGACAGAGGTGTCTGAT TTTCTCCAAACTGATTGTATTCCA CACCTGCAAAGTGGAATCAA TCTAACTTCGGCTTGGGAAA CDe CD CD NM_768 NM_ NM_886 Supplemental Table. Primers used for qpcr.
15 MATERIALS AND METHODS SUPPLEMENTAL INFORMATION Intraperitoneal glucose tolerance test (ipgtt) and iulin tolerance test (ITT). For glucose and iulin tolerance tests, g/kg glucose or.7 IU/kg iulin (Actrapid; Novo Nordisk), respectively, was injected intraperitoneally after a 6-h fast and blood glucose was measured (Accu-Check; Roche Diagnostics) at indicated time points. Preparation of stromal vascular fraction cells and isolation of preadipocytes. Epididymal adipose tissue was cut into small pieces and digested at 7 C for min with collagenase type I (Sigma) followed by centrifugation at g for min. Cell pellets were suspended in ice-cold x PBS and filtered through a 7 µm-sieve, and the SVF fraction was collected by centrifugation at g for min. Cells were stained with rat antibody agait CD (clone -F, BD Biosciences), CD (clone 9, BioLegend), Sca- (clone D7, ebioscience), CD (clone HM, BioLegend), CD (clone M/69, BD Biosciences) and CD9 (clone HMB-, BioLegend). Preadipocytes were identified as CD - CD - Sca- + CD9 + CD + CD - cells () and sorted using the FACSAria (BD Bioscience) cell sorter, or analysed using the LSRII Fortessa (BD Bioscience) and FlowJo software (Tree Star Inc). The purity of isolated preadipocytes was > 9% after cell sorting.. Berry R, Rodeheffer MS. Characterization of the adipocyte cellular lineage in vivo. Nat Cell Biol ;():-8.
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