Step 4. Step 4. Choose MW markers. Choose MW markers. Gel Electrophoresis of Proteins

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "Step 4. Step 4. Choose MW markers. Choose MW markers. Gel Electrophoresis of Proteins"

Transcription

1 Gel Electrophoresis of Proteins Choose MW markers 16 Step 4 Gel Electrophoresis of Proteins A Practical Approach, Third Edition An overview of basic techniques to benefit any life scientist using electrophoretic methods. Includes emerging techniques such as capillary gel electrophoresis, sequence analysis of gel-resolved proteins, fluorophorelabeled saccharide electrophoresis, and use of gel electrophoresis for analysis of protein:protein interactions. Step 4 Choose MW markers Molecular Weight Markers To assess relative molecular weight of a protein on a gel, protein molecular weight markers are run in outer lanes of for comparison. A standard curve can be constructed from distances migrated by each marker protein. The distance migrated by unknown protein is n plotted and molecular weight is interpolated from standard curve. Pierce offers a variety of molecular weight (MW) markers for use with one- and twodimensional gels and for various detection methods. Table 3 summarizes different features of each marker mix. These protein marker mixes are available unstained for in-gel staining with any protein stain or as prestained markers that can be visualized in a gel or when transferred to a membrane. The most convenient feature of Ranger Molecular Weight Markers is unique room temperature-stable, single-dose tube packaging. There is no need to aliquot and freeze marker formulation because it is already dispensed into a 48-microtube tray. Simply puncture protective foil layer and add running buffer to rehydrate proteins. Then transfer 2-10 µl of mix to a lane on. The 2-D Protein Molecular Weight Marker Mix (Product # 26659) provides a unique protein formulation with a wide pi range ( ) for analysis in first dimension and a wide MW range (17 K-80 K) for second dimension. The Chemiluminescent BlueRanger Protein Molecular Weight Marker Mix (Product # 26651) has been created for chemiluminescent detection on Western blots. This MW marker shows up in-gel and on a membrane because it is prestained. It also can be detected on film using a chemiluminescent substrate for HRP because it is peroxidase-labeled. Hames, B.D., Ed., Published by Oxford University Press, 1998, 352 pages, Soft-cover Product #: 20044

2 Electrophoresis & Staining Flowchart 1. sample Buffers Choose MW markers Table 3. Pierce protein molecular weight markers products for electrophoresis.* Run Stain Poststaining BlueRanger TriChromRanger ColorMeRanger Chemiluminescent DyLight MW Markers Markers Markers BlueRanger Markers 2-D MW Marker Mix Marker Mix Protein (Product # 26681) (Product # 26691) (Product # 26671) (Product # 26651) (Product # 26659) (Product # 26665) Myosin 210 K 210 K 200 K 220 K 200 K Phosphorylase B 120 K 110 K 97.4 K 104 K 97 K ApoTransferrin 80 K Bovine Serum 84 K 80 K 66K 76 K 66 K Albumin (BSA) Glutamic 56 K Dehydrogenase Ovalbumin 60 K 47 K 43 K 45 K Protein A 42 K Actin 43 K Carbonic Anhydrase 39.2 K 32 K 29 K 33 K 29 K Protein L 36 K Myokinase 22.5 K Peanut Agglutinin 28 K Soybean Trypsin 28 K 25 K 20 K 26 K 20 K 20 K Inhibitor Myoglobin 17 K Lysozyme 18.3 K 16.5 K 14.4 K 18 K 14 K Aprotinin 6 K 6 K Insulin B Chain 3.5 K Staining Feature Prestained (1 color) Prestained (3 colors) Unstained Peroxidase labeled Unstained Prestained and prestained (2 fluors) (1 color) Package 48 microtubes 48 microtubes 48 microtubes 48 microtubes 500 µl (~250 gels) 250 µl (50 gels) *Actual molecular weights are lot-specific because proteins are prestained. Lot-specific information is included in each package. New! Step 4 Choose MW markers 17 BlueRanger Prestained Molecular Weight Markers 15,16 A fresh marker every time, not just first time. A totally new idea in how molecular weight markers are packaged! Innovative single-dose package Room temperature stable Excellent performance on wide range of gel compositions Efficient membrane transfer Single-dose packaging in a novel microtube plate format eliminates opportunities for contamination due to multiple marker withdrawals from same vial Unique stabilized prestained markers can be stored at room temperature Compatible with a broad range of SDS-PAGE gel compositions and downstream applications Prestained proteins transfer well to both nitrocellulose and PVDF membrane Can be used with GelCode Blue, SilverSNAP and E-Zinc Stains Formulated to yield prestained protein bands of equal intensity Here s how it works: BlueRanger Prestained Markers are an individually dried and stabilized formulation of seven proteins spanning range from 18.3 K to 210 K. The plate is covered with a foil that can be easily punctured with a pipette tip. Simply puncture foil covering a single well containing dried marker mix with a pipette tip and add 10 µl deionized water. The marker proteins are reconstituted instantly and ready for loading onto a gel lane. The proteins listed, covering a broad molecular weight range, have been prestained and purified to give single bands on 4-20% SDS-PAGE gels. Each protein has been proportioned into mix to yield uniform band intensity. Tel: or

3 Step 4 Choose MW markers Gel Electrophoresis of Proteins BlueRanger Prestained Molecular Weight Markers, continued Markers are ready when you are and room temperature-stable. 1. Open resealable plastic pouch and remove BlueRanger Prestained Protein Molecular Weight Marker Mix. BlueRanger Prestained Marker Mix is packaged with a desiccant in a moisture-resistant, resealable pouch. 2. Load 10 µl of DI water into a pipette tip, puncture foil over a single tube and dissolve BlueRanger Prestained Markers. 3. Dispense 5-10 µl of marker into a sample well of to be run. Each tube can be used to prepare one or two lanes of a gel. BlueRanger Prestained Marker Protein Molecular Weights Myosin Phosphorylase B Serum Albumin Ovalbumin Carbonic Anhydrase Trypsin Inhibitor Lysozyme 210 K 120 K 84 K 60 K 39.2 K 28 K 18.3 K Return BlueRanger Prestained Marker Mix to its pouch and reseal. The markers are stable at room temperature and can be kept right on your bench-top ready for your next SDS-PAGE gel. References 15. Foubert, T.R., et al. (2001). J. Biol. Chem. 276, Prozialeck, W.C., et al. (2002). Infect. Immun. 70, BlueRanger Prestained Protein 1 x 48 Molecular Weight Marker Mix microtube Sufficient material for loading plate gel lanes BlueRanger Prestained Protein 5 x 48 Molecular Weight Marker Mix microtube Sufficient material for loading plates gel lanes. TriChromRanger Prestained Molecular Weight Markers 17,18 Fresh marker every time, with reference bands too. BlueRanger Marker Myosin (210 K) Phosphorylase B BSA Ovalbumin TriChromRanger Marker Innovative single-dose packaging allows you to dissolve only marker you need exactly when you want it The single-dose packaging eliminates possibility of contamination due to multiple withdrawals Room-temperature storage eliminates need to expose protein markers to detrimental freeze-thaw cycles References 17. Myers, C.R. and Myers, J.M. (2002). Appl. Envir. Microbiol. 68, Cui, L., et al. (2002). Am. J. Physiol. Cell Physiol. 283, C623-C630. Carbonic Anhydrase Trypsin Inhibitor Lysozyme (16.5 K) Each tube of TriChromRanger Marker consists of a stabilized and lyophilyzed formulation of seven proteins, ranging from 16.5 K to 210 K. Each protein in mixture is proportioned to yield uniform band intensities. Two specially modified bands (one red, one violet) serve as references for order of marker proteins TriChromRanger Prestained Protein 1 x 48 Molecular Weight Marker Mix microtube Sufficient material for loading gel lanes. plate For more product information, or to download a product instruction booklet, visit

4 Electrophoresis & Staining Flowchart 1. sample Buffers Choose MW markers Chemiluminescent BlueRanger Molecular Weight Markers Run Stain Poststaining New protein molecular weight standard looks and acts like a typical pre-stained marker for SDS-PAGE and can also light up after transfer or in-gel. Colorimetric and chemiluminescent two detection options are available: on-membrane or in-gel Visual detection in-gel already prestained; does not require staining to detect in-gel Universal compatibility with HRP conjugates self-contained peroxidase activity, does not require an HRP-antibody conjugate for chemiluminescence and no variability due to host animal or antibody class Compatible with streptavidin-hrp conjugates Room temperature stable Convenient packaging single dose in 48-well microtube plate The Chemiluminescent BlueRanger Marker consists of seven proteins spanning molecular weight range from 18 K to 220 K. Each marker component is prestained covalently with a blue dye followed by chemical modification imparting peroxidase capability. Unlike any or chemiluminescent detection-compatible marker for Western blot applications, Chemiluminescent BlueRanger Marker does not depend on binding of an HRP-antibody conjugate to it to yield a chemiluminescent signal. Component Proteins Myosin Heavy Chain Phosphorylase B BSA Ovalbumin Carbonic Anhydrase Trypsin Inhibitor Lysozyme A. B. Colorimetric and Chemiluminescent Detection on a Western Blot MW of Chemiluminescent BlueRanger Proteins* 220 K 104 K 76 K 45 K 33 K 26 K 18 K Colorimetric and Chemiluminescent Detection In-Gel using UnBlot Technology *These are representative molecular weight values. The covalently bound dye and enzyme alter apparent molecular weight (MW) of component proteins relative to ir unstained counterparts. Lot-specific MW values are provided with each package. Figure 5. A. Western blot detection. Lanes 1-4 show marker run on a 4-20% Tris-Glycine SDS-polyacrylamide gel and transferred to nitrocellulose. Lanes 1 and 3 were loaded with 2 µl of marker. Lanes 2 and 4 were loaded with 5 µl of marker. Lanes 1 and 2 marker colorimetrically after transfer to membrane. Lanes 3 and 4 were treated with SuperSignal West Pico Chemiluminescent Substrate (Product # 34080) and exposed to X-ray film for 1 minute. B. In-gel detection (UnBlot Technology ). Lanes 1 and 2 were each loaded with 10 µl of marker before electrophoresis on a 4-20% Tris-Glycine gel. Lane 1 shows marker bands colorimetrically in-gel. Lane 2 shows marker proteins detected in-gel using UnBlot Technology with UnBlot Chemiluminescent Substrate (Product # 33550) and exposure of to X-ray film for one minute. Chemiluminescent marker optimized for Western blot and Pierce UnBlot Applications Figure 6. Pierce Chemiluminescent BlueRanger Markers (5 µl and 2 µl loading) (Lanes 1 and 2) and two 6xHis proteins (~10 ng) (Lanes 3 and 4) were separated by electrophoresis using a 4-20% Tris-Glycine gradient gel. The proteins were transferred to nitrocellulose and detected for 6xHis tag using SuperSignal West HisProbe Kit (Product # 15168). The blot was exposed to X-ray film for 1 minute to capture chemiluminescent signal. (The blot was scanned to document color.) Chemiluminescent BlueRanger 1 x 48 Prestained Peroxidase-Labeled microtube Protein Molecular Weight Marker Mix plate CAUTION: Chemiluminescent BlueRanger Markers are prelabeled with a peroxidase enzyme. This means y must be handled more gently than traditional prestained markers. These markers can be overheated to point of inactivation during transfer from to membrane. They can also be inactivated by or conditions that are detrimental to peroxides such as too much EDTA/EGTA, azide or acidic membrane stains such as Ponceau S. In most systems, inactivation is unlikely to occur, but if it does occur in your system, please return remaining product for a full refund. Step 4 Choose MW markers 19 Tel: or

5 Step 4 Choose MW markers 20 Gel Electrophoresis of Proteins 2-D Protein Molecular Weight Markers A 2-D gel marker mix that offers a broad range of pi and MW. The new ready-to-use 2-D gel marker mix from Pierce is designed specifically to aid proteome analyst. This 2-D gel marker mix contains a complement of seven reduced and denatured proteins. When performing protein 2-D separation and analysis, each protein in mix provides important features for assessing system performance or estimating pi and molecular weight values. It s also convenient this marker mix doesn t require you to sign a notarized document just to use it. A unique complement of proteins Each protein in this marker mix was carefully selected to give a useful range of molecular weight and pi coverage. Proteins were selected that give a variety of features from tight single spots to characteristic charge trains to aid analyst in gel orientation. Molecular weights range from 17 K to 80 K, with pi values ranging from 4.5 to 8.7. Component Proteins in new Pierce 2-D Protein Molecular Weight Marker Mix 2-D Marker Protein MW pi Value Apo-Transferrin (human plasma) 80 K 6.2 Glutamic Dehydrogenase (bovine liver) 56 K 6.5, 6.7, 6.9 Actin (bovine muscle) 43 K 5.2 Carbonic Anhydrase (bovine erythrocytes) 29 K 6.3 Myokinase (chicken muscle) 22.5 K 8.7 Trypsin Inhibitor (soybean) 20 K 4.5 Myoglobin (equine skeletal muscle) 17 K 7.0, 7.4 F C A D B A. Human Apo-Transferrin E. Chicken Myokinase B. Bovine Glutamic Dehydrogenase F. Soybean Trypsin Inhibitor C. Bovine Muscle Actin G. Equine Myoglobin D. Bovine Carbonic Anhydrase Figure 7. 2-D markers shown stained with silver (left) and coomassie blue dye (right). 500 µl of Pierce 2-D Marker Mix is sufficient for following number of applications, depending on gel size and staining method Stain Marker # of Gels/ Gel Size Method Volume Vial Mini-Gels Coomassie Blue Dye 2.5 µl 200 Mini-Gels Silver µl 500-1,000 Large Format Gels Coomassie Blue Dye µl Large Format Gels Silver µl The 2-D Protein Molecular Weight Marker Mix is supplied frozen. For optimal long-term stability, aliquot into sample vials upon receipt and refreeze. G E F C A D B G E D Protein Molecular Weight 500 µl Marker Mix For more product information, or to download a product instruction booklet, visit

6 Electrophoresis & Staining Flowchart 1. sample Buffers Choose MW markers DyLight Fluorescent Protein Molecular Weight Markers One- or two-color fluorescent detection with one protein molecular weight marker. DyLight Fluorescent Protein Molecular Weight Markers are optimized for direct visualization of marker proteins after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Each protein in mixture is double-labeled with DyLight 547 and DyLight 647 Fluorescent Dyes to provide flexible one- or two-color detection with common imaging systems (Figure 8). The markers are compatible with Western blotting (Figure 9) and can be detected by virtually any in-gel staining method (Figure 10). The DyLight Fluorescent Protein Molecular Weight Markers consists of nine proteins with molecular weights in range of 6 K to 200 K. Fluorescent and Colorimetric Detection of DyLight Fluorescent Protein Molecular Weight Markers Myosin (200 K) Phosphorylase B (97 K) BSA (66 K) Protein A (42 K) Protein L (36 K) Peanut Agglutinin (28 K) Trypsin Inhibitor (20 K) Lysozyme (14 K) Aprotinin (6 K) Figure 8 A B Figure 9 A B Figure 10 A B Run Stain Poststaining Easily multiplexed two excitation and emission maxima enable one- or two-color fluorescent detection Easy to use and convenient eliminate need for awkward marking or overlay procedures Fluorescent and colorimetric two detection options available: in-gel or on-membrane Instrument-compatible DyLight Dye spectra are compatible with common imaging systems Photostable allows long exposure times for maximum sensitivity Table 4. Spectral characteristics of DyLight Fluorescent Protein Molecular Weight Markers. Extinction Excitation (nm) Emission (nm) Coefficient (min) DyLight 547 Dye ,000 M -1 cm -1 DyLight 647 Dye ,000 M -1 cm DyLight Fluorescent Protein 250 µl Molecular Weight Markers Sufficient material for loading 50 gel lanes. Step 4 Choose MW markers 21 Figure 8. Direct in-gel fluorescent detection. Marker proteins (5 µl) were separated in 4-20% Tris-glycine gels. The gels were imaged with Kodak Image Station 2000MM using a five-minute exposure at f2.8 with A. 535/600 nm excitation/emission filter set or B. 625/700 nm excitation/ emission filter set. Figure 9. Fluorescent Western blot detection. Marker proteins (5 µl) were separated in 4-20% Tris-glycine gels and transferred to A. nitrocellulose or B. PVDF membrane. Blots were imaged with Typhoon 9410 at 500V PMT using A. Cy 3 Dye or B. Cy 5 Dye laser settings. Note: Proteins in marker mix produce uniform fluorescent intensities in SDS-PAGE applications; however, variations in protein-transfer efficiency affect fluorescent intensity. For example, high molecular weight proteins, such as myosin (200 K), typically transfer less efficiently than low molecular weight proteins. Figure 10. Colorimetric in-gel detection. Marker proteins (10 µl) were separated in 4-20% Tris-glycine gels and stained with A. Imperial Protein Stain or B. silver stain. Tel: or

HiPer Western Blotting Teaching Kit

HiPer Western Blotting Teaching Kit HiPer Western Blotting Teaching Kit Product Code: HTI009 Number of experiments that can be performed: 5/20 Duration of Experiment: ~ 2 days Day 1: 6-8 hours (SDS- PAGE and Electroblotting) Day 2: 3 hours

More information

Apolipoprotein A-1 ELISA

Apolipoprotein A-1 ELISA Apolipoprotein A-1 ELISA For the quantitative determination of apolipoprotein A1 in serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures. Please read carefully due to Critical

More information

Multiplex Protein Quantitation using itraq Reagents in a Gel-Based Workflow

Multiplex Protein Quantitation using itraq Reagents in a Gel-Based Workflow Multiplex Protein Quantitation using itraq Reagents in a Gel-Based Workflow Purpose Described herein is a workflow that combines the isobaric tagging reagents, itraq Reagents, with the separation power

More information

ab Glycated Protein Detection Kit

ab Glycated Protein Detection Kit Version 1 Last updated 27 July 2017 ab219802 Glycated Protein Detection Kit For the detection of glycated proteins (AGEs) using a simple polyacrylamide gel electrophoresis-based method. This product is

More information

STAT3 (py705)/ Pan STAT3 (Human/Mouse/Rat) ELISA Kit

STAT3 (py705)/ Pan STAT3 (Human/Mouse/Rat) ELISA Kit STAT3 (py705)/ Pan STAT3 (Human/Mouse/Rat) ELISA Kit Catalog Number KA2176 96 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Principle of the Assay...

More information

STAT3 (py705) (Human/Mouse/Rat) ELISA Kit

STAT3 (py705) (Human/Mouse/Rat) ELISA Kit STAT3 (py705) (Human/Mouse/Rat) ELISA Kit Catalog Number KA2175 96 assays Version: 01 Intended for research use only www.abnova.com I. INTRODUCTION STAT3 (py705) (Human/Mouse/Rat) ELISA (Enzyme-Linked

More information

Mouse serum Insulin 200 tests

Mouse serum Insulin 200 tests Headquarters & Europe Office Cisbio Bioassays Phone: +33 (0)4 66 79 67 05 Fax: +33 (0)4 66 79 19 20 bioassays@cisbio.com cisbio_dd_pi_62in3pef-2µl USA Office Cisbio US, Inc. Phone: +1 888 963 4567 Fax:

More information

ExoTEST Ready to Use Kit for ELISA Exosome quantification

ExoTEST Ready to Use Kit for ELISA Exosome quantification Datasheet ExoTEST Ready to Use Kit for ELISA Exosome quantification This product is for research use only. It is highly recommended to read this users guide in its entirety prior to using this product.

More information

RayBio Human ENA-78 ELISA Kit

RayBio Human ENA-78 ELISA Kit RayBio Human ENA-78 ELISA Kit Catalog #: ELH-ENA78 User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,

More information

TECHNICAL BULLETIN. Catalog Number RAB0447 Storage Temperature 20 C

TECHNICAL BULLETIN. Catalog Number RAB0447 Storage Temperature 20 C Phospho-Stat3 (ptyr 705 ) and pan-stat3 ELISA Kit for detection of human, mouse, or rat phospho-stat3 (ptyr 705 ) and pan-stat3 in cell and tissue lysates Catalog Number RAB0447 Storage Temperature 20

More information

Ralstonia solanacearum PathoScreen Kit DAS ELISA, peroxidase label Catalog number: PSP 33900

Ralstonia solanacearum PathoScreen Kit DAS ELISA, peroxidase label Catalog number: PSP 33900 List of contents Ralstonia solanacearum PathoScreen Kit Lot number Item 96 wells 288 wells 480 wells Antibody coated 96-well microtiter plates 1 3 5 Peroxidase enzyme conjugate, ready to use 11 ml 33 ml

More information

In-Gel Tryptic Digestion Kit

In-Gel Tryptic Digestion Kit INSTRUCTIONS In-Gel Tryptic Digestion Kit 3747 N. Meridian Road P.O. Box 117 Rockford, IL 61105 89871 1468.2 Number Description 89871 In-Gel Tryptic Digestion Kit, sufficient reagents for approximately

More information

Superior Fluorescent Labeling Dyes Spanning the Full Visible Spectrum...1. Trademarks: HiLyte Fluor (AnaSpec, Inc.)

Superior Fluorescent Labeling Dyes Spanning the Full Visible Spectrum...1. Trademarks: HiLyte Fluor (AnaSpec, Inc.) Table of Contents Fluor TM Labeling Dyes Superior Fluorescent Labeling Dyes Spanning the Full Visible Spectrum....1 Fluor TM 405 Dye, an Excellent Alternative to Alexa Fluor 405 & DyLight 405....2 Fluor

More information

MW.SDS.70L and MW-SDS.200 Kits

MW.SDS.70L and MW-SDS.200 Kits ~'A'.'.A'k'~ ~ ~ ':if';"7'~~'!11;~\ C HEM IC A I CQ P.O. ~X 14508,$T,LQV1S,MQ;, ~17,;U$A SDS MOLECULAR WEIGHT MARKERS IN A DISCONTINUOUS BUFFER July 1988 Technical Bulletin No. MWS-877L ORDER DIRECT: USA/Canada

More information

02006B 1 vial 02006B 1 vial Store at -20 C. Lyophilized recombinant IL-2

02006B 1 vial 02006B 1 vial Store at -20 C. Lyophilized recombinant IL-2 For detection and measurement of human interleukin 2 Catalog #02006 Catalog #02007 2 Plates 10 Plates Product Description The Human Interleukin 2 (IL-2) ELISA Kit is designed for the quantitative detection

More information

TECHNICAL BULLETIN. Phospho-Akt (pser 473 ) ELISA Kit for detection of human, mouse, or rat phospho-akt (pser 473 ) in cell and tissue lysates

TECHNICAL BULLETIN. Phospho-Akt (pser 473 ) ELISA Kit for detection of human, mouse, or rat phospho-akt (pser 473 ) in cell and tissue lysates Phospho-Akt (pser 473 ) ELISA Kit for detection of human, mouse, or rat phospho-akt (pser 473 ) in cell and tissue lysates Catalog Number RAB0011 Storage Temperature 20 C TECHNICAL BULLETIN Product Description

More information

Mercodia Glucagon ELISA

Mercodia Glucagon ELISA Mercodia Glucagon ELISA Directions for Use 10-1271-01 REAGENTS FOR 96 DETERMINATIONS For Research Use Only Manufactured by Mercodia AB, Sylveniusgatan 8A, SE-754 50 Uppsala, Sweden EXPLANATION OF SYMBOLS

More information

Anti-TSH-Receptor ELISA

Anti-TSH-Receptor ELISA Instructions for Use Anti-TSH-Receptor ELISA Enzyme Immuno Assay for the Quantitative Determination of TSH Receptor Autoantibodies (TRAb) in Serum I V D REF EA101/96 12 x 8 2 8 C DLD Gesellschaft für Diagnostika

More information

TABLE OF CONTENTS GENERAL INFORMATION... 1

TABLE OF CONTENTS GENERAL INFORMATION... 1 BIOO RESEARCH PRODUCTS Glucose Assay Kit Manual Catalog #: 5611-01 BIOO Scientific Corp. 2011 TABLE OF CONTENTS GENERAL INFORMATION... 1 Product Description... 1 Procedure Overview... 1 Required Materials

More information

ab TSH Receptor Autoantibody ELISA Kit

ab TSH Receptor Autoantibody ELISA Kit ab178661 TSH Receptor Autoantibody ELISA Kit Instructions for Use For the quantitative measurement of TSH Receptor Autoantibodies in Human serum. This product is for research use only and is not intended

More information

Electrophoresis Products

Electrophoresis Products Electrophoresis Products Special Offer ltd HORIZONTAL GEL SYSTEMS VERTICAL GEL SYSTEMS BLOTTING OPTIONS POWER SUPPLIES PIPETTES GEL CASTING KITS & PRECAST GELS GEL SOLUTIONS & BUFFERS TRANSFER MEMBRANES

More information

ECL Plex Western Blotting Detection System

ECL Plex Western Blotting Detection System Part of GE Healthcare Data File 28-415-39 AA ECL Plex Western Blotting Detection System Multiplex protein detection based on direct fluorescent CyDye-labeled conjugates ECL Plex fluorescent Western blotting

More information

Aspartate Transaminase (AST) Color Endpoint Assay Kit Manual Catalog #:

Aspartate Transaminase (AST) Color Endpoint Assay Kit Manual Catalog #: Aspartate Transaminase (AST) Color Endpoint Assay Kit Manual Catalog #: 5605-01 TABLE OF CONTENTS GENERAL INFORMATION... 2 Product Description... 2 Procedure Overview... 2 Kit Contents, Storage and Shelf

More information

ab alpha 1 Microglobulin Human ELISA Kit

ab alpha 1 Microglobulin Human ELISA Kit ab108884 alpha 1 Microglobulin Human ELISA Kit Instructions for Use For the quantitative measurement of Human alpha 1 Microglobulin in plasma, serum, urine, saliva, milk and cell culture supernatants.

More information

HIV-1 p24 ELISA Kit. Cat.No: DEIA10155 Lot. No. (See product label) Size. Storage. Principle Of The Test. Reagents And Materials Provided

HIV-1 p24 ELISA Kit. Cat.No: DEIA10155 Lot. No. (See product label) Size. Storage. Principle Of The Test. Reagents And Materials Provided HIV-1 p24 ELISA Kit Cat.No: DEIA10155 Lot. No. (See product label) Size 96T Storage All reagents should be stored at 2-8 C, and should not be used beyond the expiration date on the label. Once opened,

More information

ab C-Peptide ELISA Kit

ab C-Peptide ELISA Kit ab178641 C-Peptide ELISA Kit Instructions for Use An immunoenzymatic assay for the quantitative measurement of C-Peptide in Human serum and plasma. This product is for research use only and is not intended

More information

2009 H1N1 Influenza ( Swine Flu ) Hemagglutinin ELISA kit

2009 H1N1 Influenza ( Swine Flu ) Hemagglutinin ELISA kit 2009 H1N1 Influenza ( Swine Flu ) Hemagglutinin ELISA kit Catalog Number : SEK001 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as

More information

HIV-1 p24 ELISA Kit. Catalog Number KA assays Version: 06. Intended for research use only.

HIV-1 p24 ELISA Kit. Catalog Number KA assays Version: 06. Intended for research use only. HIV-1 p24 ELISA Kit Catalog Number KA0452 96 assays Version: 06 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Principle of the Assay... 3 General Information... 4 Materials

More information

NF-κB p65 (Phospho-Thr254)

NF-κB p65 (Phospho-Thr254) Assay Biotechnology Company www.assaybiotech.com Tel: 1-877-883-7988 Fax: 1-877-610-9758 NF-κB p65 (Phospho-Thr254) Colorimetric Cell-Based ELISA Kit Catalog #: OKAG02015 Please read the provided manual

More information

Branched Chain Amino Acid (Leu, Ile, Val) Assay Kit

Branched Chain Amino Acid (Leu, Ile, Val) Assay Kit Branched Chain Amino Acid (Leu, Ile, Val) Assay Kit Catalog Number KA0824 100 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General

More information

TECHNICAL BULLETIN. Ghrelin EIA Kit for serum, plasma, culture supernatant, and cell lysates. Catalog Number RAB0207 Storage Temperature 20 C

TECHNICAL BULLETIN. Ghrelin EIA Kit for serum, plasma, culture supernatant, and cell lysates. Catalog Number RAB0207 Storage Temperature 20 C Ghrelin EIA Kit for serum, plasma, culture supernatant, and cell lysates Catalog Number RAB0207 Storage Temperature 20 C TECHNICAL BULLETIN Product Description The Ghrelin EIA (Enzyme Immunoassay) Kit

More information

ab Human GFAP SimpleStep ELISA Kit

ab Human GFAP SimpleStep ELISA Kit Version 1 Last updated 14 August 2017 ab223867 Human GFAP SimpleStep ELISA Kit For the quantitative measurement of GFAP in tissue extracts and cell extracts This product is for research use only and is

More information

Blood Urea Nitrogen Enzymatic Kit Manual Catalog #:

Blood Urea Nitrogen Enzymatic Kit Manual Catalog #: Blood Urea Nitrogen Enzymatic Kit Manual Catalog #: 5602-01 TABLE OF CONTENTS GENERAL INFORMATION... 2 Product Description... 2 Procedure Overview... 2 Kit Contents, Storage and Shelf Life... 3 Required

More information

Vedolizumab Drug Level ELISA

Vedolizumab Drug Level ELISA Vedolizumab Drug Level ELISA For the quantitative determination of free Entyvio concentration in human serum and EDTA plasma. Please read carefully due to Critical Changes, e.g., Updates to specificity

More information

HBeAg and HBeAg Ab ELISA Kit

HBeAg and HBeAg Ab ELISA Kit HBeAg and HBeAg Ab ELISA Kit Catalog Number KA0290 96 assays Version: 17 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3

More information

Mercodia Porcine C-peptide ELISA

Mercodia Porcine C-peptide ELISA Mercodia Porcine C-peptide ELISA Directions for Use 10-1256-01 REAGENTS FOR 96 DETERMINATIONS Manufactured by Mercodia AB, Sylveniusgatan 8A, SE-754 50 Uppsala, Sweden EXPLANATION OF SYMBOLS USED ON LABELS

More information

ab83375 Sialic Acid (NANA) Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Sialic Acid (NANA) in various samples.

ab83375 Sialic Acid (NANA) Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Sialic Acid (NANA) in various samples. ab83375 Sialic Acid (NANA) Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Sialic Acid (NANA) in various samples. Version 2 Last Updated 4 March 2015 This product is

More information

Mercodia Porcine C-peptide ELISA

Mercodia Porcine C-peptide ELISA Mercodia Porcine C-peptide ELISA Directions for Use 10-1256-01 REAGENTS FOR 96 DETERMINATIONS Manufactured by Mercodia AB Sylveniusgatan 8A SE-754 50 Uppsala Sweden INTENDED USE Mercodia Porcine C-peptide

More information

TNF-alpha ELISA. For Research Use Only. Not For Use In Diagnostic Procedures.

TNF-alpha ELISA. For Research Use Only. Not For Use In Diagnostic Procedures. TNF-alpha ELISA For the quantitative determination of TNF-alpha in serum, plasma, buffered solution or cell culture medium. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number:

More information

ENA screen (Extractable Nuclear Antigen) ELISA

ENA screen (Extractable Nuclear Antigen) ELISA ENA screen (Extractable Nuclear Antigen) ELISA Catalog Number: ENA31-K01 Enzyme immunoassay for the determination of IgG antibodies to nuclear and cytoplasmic antigens in human serum and plasma. 20A Northwest

More information

Thyroxine (T4) Human ELISA Kit

Thyroxine (T4) Human ELISA Kit ab108662 Thyroxine (T4) Human ELISA Kit Instructions for Use For the quantitative measurement of Human Thyroxine (T4) concentrations in serum. This product is for research use only and is not intended

More information

For the qualitative determination of IgM class antibodies against Parvovirus B19 in Human serum or plasma (citrate)

For the qualitative determination of IgM class antibodies against Parvovirus B19 in Human serum or plasma (citrate) ab108760 Parvovirus B19 IgM Human ELISA Kit Instructions for Use For the qualitative determination of IgM class antibodies against Parvovirus B19 in Human serum or plasma (citrate) This product is for

More information

ab Thyroxine (Total T4) ELISA Kit

ab Thyroxine (Total T4) ELISA Kit ab178664 Thyroxine (Total T4) ELISA Kit Instructions for Use A competitive immunoenzymatic assay for the quantitative measurement of Thyroxine (Total T4) in Human serum and plasma. This product is for

More information

Mycoplasma pneumoniae IgG ELISA Kit

Mycoplasma pneumoniae IgG ELISA Kit Mycoplasma pneumoniae IgG ELISA Kit Catalog Number KA2260 96 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle

More information

Helicobacter pylori IgA ELISA Kit

Helicobacter pylori IgA ELISA Kit Helicobacter pylori IgA ELISA Kit Catalog Number KA0964 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle

More information

Estriol ELISA kit. Catalog Number KA assays Version: 05. Intended for research use only.

Estriol ELISA kit. Catalog Number KA assays Version: 05. Intended for research use only. Estriol ELISA kit Catalog Number KA0316 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay... 3 General Information...

More information

ab HMG-CoA Reductase Activity Assay Kit (Colorimetric)

ab HMG-CoA Reductase Activity Assay Kit (Colorimetric) ab204701 HMG-CoA Reductase Activity Assay Kit (Colorimetric) Instructions for Use For rapid, sensitive and accurate detection of HMG-CoA Reductase Activity. This product is for research use only and is

More information

7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit

7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit 7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit Item No. 600120 www.caymanchem.com Customer Service 800.364.9897 Technical Support 888.526.5351 1180 E. Ellsworth Rd Ann Arbor, MI USA TABLE OF CONTENTS

More information

See external label 96 tests HSV 2 IgA. Cat #

See external label 96 tests HSV 2 IgA. Cat # DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external

More information

ab NFĸB p65 (ps536) + Total NFĸB p65 SimpleStep ELISA Kit

ab NFĸB p65 (ps536) + Total NFĸB p65 SimpleStep ELISA Kit ab176663 NFĸB p65 (ps536) + Total NFĸB p65 SimpleStep ELISA Kit Instructions for Use For the semi-quantitative measurement of NFĸB p65 (ps536) and NFĸB p65 (Total) in Human and mouse cell lysates. This

More information

Cytomegalovirus IgG (CMV IgG) Human ELISA Kit

Cytomegalovirus IgG (CMV IgG) Human ELISA Kit ab108724 Cytomegalovirus IgG (CMV IgG) Human ELISA Kit Instructions for Use For the qualitative determination of IgG class antibodies against Cytomegalovirus (CMV) in Human serum or plasma (citrate) This

More information

ab Apolipoprotein A1 (APOA1) Human SimpleStep ELISA Kit

ab Apolipoprotein A1 (APOA1) Human SimpleStep ELISA Kit ab189576 Apolipoprotein A1 (APOA1) Human SimpleStep ELISA Kit Instructions for Use For the quantitative measurement of Apolipoprotein A1 (APOA1) in human serum, plasma, and cell culture supernatants. This

More information

L-Amino Acid Assay Kit

L-Amino Acid Assay Kit ab65347 L-Amino Acid Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of L-Amino Acid levels in various samples. This product is for research use only and is not intended

More information

ab CytoPainter Golgi/ER Staining Kit

ab CytoPainter Golgi/ER Staining Kit ab139485 CytoPainter Golgi/ER Staining Kit Instructions for Use Designed to detect Golgi bodies and endoplasmic reticulum by microscopy This product is for research use only and is not intended for diagnostic

More information

Parvovirus B19 IgM Human ELISA Kit

Parvovirus B19 IgM Human ELISA Kit ab108760 Parvovirus B19 IgM Human ELISA Kit Instructions for Use For the qualitative determination of IgM class antibodies against Parvovirus B19 in Human serum or plasma (citrate). This product is for

More information

Proteomics Grade. Protocol. Catalog # Agilent Technologies. Research Use Only. Not for use in Diagnostic Procedures. Version A, January 2010

Proteomics Grade. Protocol. Catalog # Agilent Technologies. Research Use Only. Not for use in Diagnostic Procedures. Version A, January 2010 Proteomics Grade Trypsin Catalog #204310 Protocol Version A, January 2010 Research Use Only. Not for use in Diagnostic Procedures. Agilent Technologies Notices Agilent Technologies, Inc. 2010 No part of

More information

SensoLyte 520 Cathepsin K Assay Kit *Fluorimetric*

SensoLyte 520 Cathepsin K Assay Kit *Fluorimetric* SensoLyte 520 Cathepsin K Assay Kit *Fluorimetric* Catalog # 72171 Kit Size 100 Assays (96-well plate) Optimized Performance: This kit is optimized to detect Cathepsin K activity. Enhanced Value: Ample

More information

Human Alpha 1 microglobulin ELISA Kit

Human Alpha 1 microglobulin ELISA Kit Human Alpha 1 microglobulin ELISA Kit Catalogue No.: EH4144 Size: 48T/96T Reactivity: Human Range:0.625-40ng/ml Sensitivity:

More information

INTACT HUMAN PROINSULIN ELISA KIT 96-Well Plate (Cat. # EZHIPI-17K )

INTACT HUMAN PROINSULIN ELISA KIT 96-Well Plate (Cat. # EZHIPI-17K ) INTACT HUMAN PROINSULIN ELISA KIT 96-Well Plate (Cat. # EZHIPI-17K ) I. Intended Use 2 II. Principles Of Procedure 2 III. Reagents Supplied 2 IV. Storage and Stability 4 V. Reagent Precautions 4 VI. Materials

More information

TSH Receptor Monoclonal Antibody (49) Catalog Number MA3-218 Product data sheet

TSH Receptor Monoclonal Antibody (49) Catalog Number MA3-218 Product data sheet Website: thermofisher.com Customer Service (US): 1 800 955 6288 ext. 1 Technical Support (US): 1 800 955 6288 ext. 441 TSH Receptor Monoclonal Antibody (49) Catalog Number MA3-218 Product data sheet Details

More information

Cereal yellow dwarf virus - RPV ELISA Kit

Cereal yellow dwarf virus - RPV ELISA Kit Cereal yellow dwarf virus - RPV ELISA Kit Cat. No.:DEIA9232 Pkg.Size:96T Intended use The Cereal yellow dwarf virus - RPV ELISA Kit is a qualitative serological assay for the detection of CYDV-RPV in plant

More information

EnzChek Myeloperoxidase (MPO) Activity Assay Kit

EnzChek Myeloperoxidase (MPO) Activity Assay Kit EnzChek Myeloperoxidase (MPO) Activity Assay Kit Catalog no. E33856 Table 1 Contents and storage Material* Amount Concentration Storage Stability 3 -(p-aminophenyl) fluorescein (APF) (Component A) 5 µl

More information

FREE THYROXINE (f-t4) CHEMILUMINESCENCE IMMUNOASSAY KIT

FREE THYROXINE (f-t4) CHEMILUMINESCENCE IMMUNOASSAY KIT FREE THYROXINE (f-t4) CHEMILUMINESCENCE IMMUNOASSAY KIT Catalog No. CL1005-2 INTENDED USE The Autobio f-t4 CLIA test kit is intended for the quantitative determination of free thyroxine (f-t4) concentration

More information

For the quantitative measurement of Human Estradiol 17-beta concentrations in plasma and serum

For the quantitative measurement of Human Estradiol 17-beta concentrations in plasma and serum ab108667 Estradiol 17-beta Human ELISA Kit Instructions for Use For the quantitative measurement of Human Estradiol 17-beta concentrations in plasma and serum This product is for research use only and

More information

CHEMILUMINESCENCE ENZYME IMMUNOASSAY (CLIA) Toxoplasma IgG. Cat # (20-25 C Room temp.) Volume

CHEMILUMINESCENCE ENZYME IMMUNOASSAY (CLIA) Toxoplasma IgG. Cat # (20-25 C Room temp.) Volume DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external

More information

ab Sialic Acid (NANA) Assay Kit (Colorimetric/Fluorometric)

ab Sialic Acid (NANA) Assay Kit (Colorimetric/Fluorometric) ab83375 - Sialic Acid (NANA) Assay Kit (Colorimetric/Fluorometric) Instructions for Use For rapid, sensitive and accurate measurement of Sialic Acid (NANA) in various samples. This product is for research

More information

LH (Bovine) ELISA Kit

LH (Bovine) ELISA Kit LH (Bovine) ELISA Kit Catalog Number KA2280 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...

More information

ab Anti-Cytomegalovirus (CMV) IgM Human ELISA Kit

ab Anti-Cytomegalovirus (CMV) IgM Human ELISA Kit ab108725 Anti-Cytomegalovirus (CMV) IgM Human ELISA Kit Instructions for Use For the qualitative measurement of IgM class antibodies against Cytomegalovirus (CMV) in Human serum and plasma (citrate). This

More information

Collagenase Assay Kit

Collagenase Assay Kit Collagenase Assay Kit Catalog # 31 and 32 For Research Use Only - Not Human or Therapeutic Use INTRODUCTION Collagenases are members of the matrix metalloproteinase (MMP) family and degrade collagen types

More information

ab65344 Uric Acid Assay Kit (Colorimetric/Fluorometric)

ab65344 Uric Acid Assay Kit (Colorimetric/Fluorometric) ab65344 Uric Acid Assay Kit (Colorimetric/Fluorometric) Instructions for Use For the rapid, sensitive and accurate measurement of uric acid in various samples. This product is for research use only and

More information

ab Anti-Thyroglobulin Human ELISA Kit

ab Anti-Thyroglobulin Human ELISA Kit ab178631 Anti-Thyroglobulin Human ELISA Kit Instructions for Use For the quantitative measurement of IgG class antibodies to Thyroglobulin in Human serum or plasma. This product is for research use only

More information

Protocol. G-LISA Rac1 Activation Assay Biochem Kit : 24 Assays (Absorbance Based) Cat. # BK128-S. cytoskeleton.com. Cytoskeleton, Inc.

Protocol. G-LISA Rac1 Activation Assay Biochem Kit : 24 Assays (Absorbance Based) Cat. # BK128-S. cytoskeleton.com. Cytoskeleton, Inc. The Protein Experts Protocol Cytoskeleton, Inc. V 8.1 G-LISA Rac1 Activation Assay Biochem Kit : 24 Assays (Absorbance Based) Cat. # BK128-S cytoskeleton.com Phone: (303) 322.2254 Fax: (303) 322.2257 Customer

More information

Human T4-HRP ELISA Kit Medical Device Licence No.: 21177

Human T4-HRP ELISA Kit Medical Device Licence No.: 21177 Human T4-HRP ELISA Kit Medical Device Licence No.: 21177 Enzyme immunoassay kit for the quantitative determination of T4 concentration in serum. Catalog Number: SL100303 96 tests For in vitro diagnostic

More information

H.pylori IgA Cat #

H.pylori IgA Cat # DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external

More information

Human Leptin ELISA Kit

Human Leptin ELISA Kit Product Manual Human Leptin ELISA Kit Catalog Numbers MET-5057 MET-5057-5 96 assays 5 x 96 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Leptin is a polypeptide hormone

More information

SensoLyte Rh110 Cathepsin K Assay Kit *Fluorimetric* Revision#1.2 Last Updated: May 2017 Catalog # Kit Size

SensoLyte Rh110 Cathepsin K Assay Kit *Fluorimetric* Revision#1.2 Last Updated: May 2017 Catalog # Kit Size SensoLyte Rh110 Cathepsin K Assay Kit *Fluorimetric* Revision#1.2 Last Updated: May 2017 Catalog # 72152 Kit Size 100 Assays (96-well plate) Optimized Performance: This kit detects Cathepsin K activity.

More information

ab Anti-Thyroid Peroxidase Human ELISA Kit

ab Anti-Thyroid Peroxidase Human ELISA Kit ab178632 Anti-Thyroid Peroxidase Human ELISA Kit Instructions for Use For the quantitative measurement of IgG class antibodies to Thyroid Peroxidase in Human serum or plasma. This product is for research

More information

E.Z.N.A. SQ Blood DNA Kit II. Table of Contents

E.Z.N.A. SQ Blood DNA Kit II. Table of Contents E.Z.N.A. SQ Blood DNA Kit II Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Blood Storage and DNA Yield...4 Preparing Reagents...5 100-500 μl Whole Blood Protocol...6

More information

An enzyme immunoassay to measure the total classical complement pathway activity in human serum SUMMARY. MicroVue CH50 Eq EIA Page 1 of 11

An enzyme immunoassay to measure the total classical complement pathway activity in human serum SUMMARY. MicroVue CH50 Eq EIA Page 1 of 11 An enzyme immunoassay to measure the total classical complement pathway activity in human serum SUMMARY MicroVue CH50 Eq EIA Page 1 of 11 INTENDED USE The MicroVue CH50 Eq EIA measures the total classical

More information

GPx Equation 1 Æ R - O - H + GSSG + H2 O

GPx Equation 1 Æ R - O - H + GSSG + H2 O OXFORD BIOMEDICAL RESEARCH P.O. Box 522, Oxford MI 48371 USA USA: 800-692-4633 Fax: 248-852-4466 www.oxfordbiomed.com Colorimetric Assay for Cellular Glutathione Peroxidase Product No. FR 17 For Research

More information

Hepatitis A virus IgM ELISA Kit

Hepatitis A virus IgM ELISA Kit Hepatitis A virus IgM ELISA Kit Catalog Number KA0285 96 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle

More information

Mitochondrial DNA Isolation Kit

Mitochondrial DNA Isolation Kit Mitochondrial DNA Isolation Kit Catalog Number KA0895 50 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials

More information

DEVELOPMENTAL VALIDATION OF SPERM HY-LITER EXPRESS Jennifer Old, Chris Martersteck, Anna Kalinina, Independent Forensics, Lombard IL

DEVELOPMENTAL VALIDATION OF SPERM HY-LITER EXPRESS Jennifer Old, Chris Martersteck, Anna Kalinina, Independent Forensics, Lombard IL DEVELOPMENTAL VALIDATION OF SPERM HY-LITER EXPRESS Jennifer Old, Chris Martersteck, Anna Kalinina, Independent Forensics, Lombard IL Background and Introduction SPERM HY-LITER is the first immunofluorescent

More information

abia T3 total ref DK ivd Note: Changes highlighted

abia T3 total ref DK ivd Note: Changes highlighted abia T3 total ref DK.033.01.3 ivd Note: Changes highlighted Intended use abia T3 total enzyme immunoassay for the quantitative determination of total triiodothyronine (T3) concentration in human serum.

More information

Human Immunodeficiency Virus type-1 p24 protein (HIV p24) Kit

Human Immunodeficiency Virus type-1 p24 protein (HIV p24) Kit TECHNICAL DATA SHEET AlphaLISA Research Reagents Caution: For Laboratory Use. A research chemical for research purposes only. Human Immunodeficiency Virus type-1 p24 protein (HIV p24) Kit Product No.:

More information

Chorionic Gonadotropin Human ELISA Kit

Chorionic Gonadotropin Human ELISA Kit ab108637 Chorionic Gonadotropin Human ELISA Kit Instructions for Use For the qualitative measurement of Human Chorionic Gonadotropin concentrations in serum and urine. This product is for research use

More information

For the rapid, sensitive and accurate measurement of Triglyceride in various samples.

For the rapid, sensitive and accurate measurement of Triglyceride in various samples. ab65336 Triglyceride Quantification Kit Instructions for Use For the rapid, sensitive and accurate measurement of Triglyceride in various samples. This product is for research use only and is not intended

More information

Acid Phosphatase Assay Kit (Fluorometric)

Acid Phosphatase Assay Kit (Fluorometric) ab83370 Acid Phosphatase Assay Kit (Fluorometric) Instructions for Use For the rapid, sensitive and accurate measurement of Acid Phosphatase in various samples This product is for research use only and

More information

Overview of the Expressway Cell-Free Expression Systems. Expressway Mini Cell-Free Expression System

Overview of the Expressway Cell-Free Expression Systems. Expressway Mini Cell-Free Expression System Overview of the Expressway Cell-Free Expression Systems The Expressway Cell-Free Expression Systems use an efficient coupled transcription and translation reaction to produce up to milligram quantities

More information

Treponema Pallidum Total Antibody ELISA

Treponema Pallidum Total Antibody ELISA For Research Use Only. Not for use in Diagnostic Procedures. INTENDED USE The GenWay, Inc. Treponima pallidum Total ELISA Kit is intended for the detection of IgG, IgM and IgA antibody to Treponima pallidum

More information

Annexin V-FITC Apoptosis Detection Kit with SYTOX

Annexin V-FITC Apoptosis Detection Kit with SYTOX ab14086 Annexin V-FITC Apoptosis Detection Kit with SYTOX Instructions for Use For the rapid, sensitive and accurate measurement of apoptosis in living cells. This product is for research use only and

More information

ab Anti-Influenza virus A IgG Human ELISA Kit

ab Anti-Influenza virus A IgG Human ELISA Kit ab108745 Anti-Influenza virus A IgG Human ELISA Kit Instructions for Use For the qualitative measurement of IgG class antibodies against Influenza virus A in Human serum and plasma (citrate). This product

More information

CYTOMEGALOVIRUS (CMV) IgM ELISA Kit Protocol

CYTOMEGALOVIRUS (CMV) IgM ELISA Kit Protocol CYTOMEGALOVIRUS (CMV) IgM ELISA Kit Protocol (Cat. No.:EK-310-91) 330 Beach Road, Burlingame CA Tel: 650-558-8898 Fax: 650-558-1686 E-Mail: info@phoenixpeptide.com www.phoenixpeptide.com INTENDED USE The

More information

IgG Antibodies To Toxoplasma Gondii ELISA Kit Protocol

IgG Antibodies To Toxoplasma Gondii ELISA Kit Protocol IgG Antibodies To Toxoplasma Gondii ELISA Kit Protocol (Cat. No.:EK-310-85) 330 Beach Road, Burlingame CA Tel: 650-558-8898 Fax: 650-558-1686 E-Mail: info@phoenixpeptide.com www.phoenixpeptide.com INTENDED

More information

Cortisol (Saliva) ELISA

Cortisol (Saliva) ELISA Cortisol (Saliva) ELISA For the quantitative determination of cortisol in human saliva. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 11CORHUE01SLV Size: 96 Wells Version:

More information

Activity # 4. Measurement of Enzyme Activity

Activity # 4. Measurement of Enzyme Activity Activity # 4. Measurement of Enzyme Activity Adapted from Experimental Molecular Biology Lab Manual, Stephen H. Munroe, Marquette University Week 1 Protein Extraction, Concentration Measurement & Instrument

More information

Campylobacter Antigen ELISA Kit

Campylobacter Antigen ELISA Kit Campylobacter Antigen ELISA Kit Catalog Number KA3204 96 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle

More information

ab Glutathione Peroxidase Assay Kit (Colorimetric)

ab Glutathione Peroxidase Assay Kit (Colorimetric) ab102530 Glutathione Peroxidase Assay Kit (Colorimetric) Instructions for Use For the rapid, sensitive and accurate measurement of glutathione peroxidase activity in various samples. This product is for

More information

CoQ10(Coenzyme Q10) ELISA Kit

CoQ10(Coenzyme Q10) ELISA Kit CoQ10(Coenzyme Q10) ELISA Kit Catalogue No.: EU0196 Size: 48T/96T Reactivity: Universal Detection Range: 0.781-50ng/ml Sensitivity:

More information