Supplementary Material. Contents include:

Size: px
Start display at page:

Download "Supplementary Material. Contents include:"

Transcription

1 Supplementary Material Contents include: 1. Supplementary Figures (p. 2-7) 2. Supplementary Figure Legends (p. 8-9) 3. Supplementary Tables (p ) 4. Supplementary Table Legends (p. 13) 1

2 Wellen_FigS1 A IL-3Ra mrna Relative expression B Glc +Glc +GlcNAc PNGase F - + Glycosylated 62 Unglycosylated 49 IL-3Ra 2

3 Wellen_FigS2 Primary Bone Marrow Cells M-NFS-60 cells (murine myeloblastic) fl *** Arbitrary Units *** 300 -Glc -Glc +GlcNAc 400 -Glc -Glc +GlcNAc 3

4 Wellen_FigS3 A. B. C. A. Cell Size B. 900 fl Cell number * 10 5 Cell Number mm Ammonia Production 4

5 Wellen_FigS4 A. B. % of glucose+ IL-3Ra Surface Expression 120 Glc- 100 Glc- GlcNAc UT CSN DJ Tun Glucose + GlcNAc pstat5 Stat5 ps6 S6 peif2a eif2a CSN Tun C. fl Cell Size Ctrl CSN DJ Tun Glc- Glc-GlcNAc+ D. Arbitrary Units IL-3Ra Surface expression *** Mgat5-wt Mgat5(L188R) 5

6 6 Wellen_FigS5

7 Wellen_FigS6 Ctrl BHA NAC GlcNAc pstat5 (Tyr 694) Stat5 7

8 Supplementary Figures Figure S1. A) IL-3-dependent cells were withdrawn from glucose for 24 hours, then treated with either 15 mm glucose, 15 mm GlcNAc, or left untreated for an additional 24 hours. RNA was isolated from triplicate wells and IL-3R gene expression normalized to that of 18S rrna. Results represent mean +/- s.d. B) Membrane protein fractions were isolated from IL-3-dependent cells after 48 hours culture in the presence of glucose. Half of each sample was treated with the deglycosylating enzyme PNGase F and then treated and untreated samples analyzed by Western blot. Figure S2. M-NSF-60 cells were withdrawn from glucose in the presence or absence of 15 mm GlcNAc for 2 days. Cell size in PI negative cells was determined by flow cytometry (mean +/- s.d. of triplicate samples). Primary bone marrow cells were withdrawn from glucose in the presence or absence of 15 mm GlcNAc for 4 days and cell size was determined in fl (mean +/- s.d. of triplicate samples; ***, p<0.0005). Figure S3. IL-3-dependent bax-/-bak-/- cells were withdrawn from glucose for 3 days in the presence of 0.5 mm or 1 mm glucosamine or 15 mm GlcNAc. Cell size (A), cell number (B), and ammonia production (C) were determined (mean +/- s.d. of triplicates). Figure S4. IL-3R surface expression is regulated by GlcNAc in a glycosylation-dependent manner. A) IL-3R surface expression determined by FACS after 2 days of incubation in glucose-free media, +/- 15 mm GlcNAc, +/- inhibitors (100 M castanospermine (CSN), 100 M deoxynojirimycin (DJ), 3 g/ml tunicamycin (tun)). Results represent mean +/ s.d. of 3 independent experiments. B) Western blots after 24 hours glucose starvation, followed by 24 hours GlcNAc addition in the presence or absence of 100 M CSN or 3 g/ml tunicamycin. Result is representative of 2 independent experiments. C) Cell size (mean +/- s.d. of triplicate wells) after 24 hours glucose starvation, followed by 24 hours GlcNAc addition, in the presence or absence of indicated inhibitors. D). Wild type Mgat5 or a mutant Mgat5 (L188R) that 8

9 fails to localize to the Golgi were stably expressed in IL-3-dependent cells. Expression and Mgat5 activity were confirmed by enzymatic assays (data not shown). IL-3R surface expression was analyzed by FACS following culture in IL-3-containing complete medium (mean +/- s.d. of triplicates, *** p<0.0005)). Figure S5. The hexosamine pathway coordinates glucose and glutamine metabolism through regulation of IL-3R surface expression in hematopoietic cells. The data suggest that glucose flux into the hexosamine pathway enables proper glycosylation and surface expression of IL-3R. IL-3 binds to IL-3R, promoting the formation of an oligomeric complex containing IL-3, IL-3R, and IL-3R c and stimulating IL-3-dependent signaling. IL-3 stimulates uptake of glutamine, in a Jak-dependent manner. Glutamine fuels cell growth through its utilization in the TCA cycle for production of ATP and precursors for fatty acid and non-essential amino acid synthesis, as well as by promoting uptake of essential amino acids to allow mtor activity and protein synthesis. In the absence of glucose, IL-3R does not express at the cell surface, inhibiting IL-3-dependent signaling, glutamine consumption, and cell growth. Figure S6. Antioxidant treatment blocks Stat5 phosphorylation in the presence of GlcNAc. Cells were withdrawn from glucose for 24 hours and then treated with GlcNAc in the presence or absence of 100 M Butylated hydroxyanisole (BHA) or 10 mm N-acetyl-L-cysteine (NAC) for an additional 24 hours. IL-3 was present in all conditions. Cells were harvested and signaling analyzed by Western blot. 9

10 Table S1: Total metabolites (Labeled + Unlabeled) Hexosamine GlcNAc-P 1h h h UDP-GlcNAc 1h h h Glycolysis Lactate 1h h h Hexose-P 1h h h PG 1h h h FBP 1h h h Pentose Phosphate Ribose-P 1h h h PRPP 1h h h TCA Succinate 1h h h Malate 1h h h Ketoglutarate 1h h h Citrate 1h h h

11 Other Glycerol-P 1h h h Glutamate 1h h h Reduced glutatione 1h h h Oxidized glutatione 1h h h N-acetyl-glutamate 1h h h Ornithine 1h h h

12 Table S2. Labeled metabolites Hexosamine no label 13 C-Glucose 13 C-GlcNAc GlcNAc-P 1h h h UDP-GlcNAc 1h h h Glycolysis Lactate 1h h h Hexose-P 1h h h PG 1h h h FBP 1h h h Pentose Phosphate Ribose-P 1h h h PRPP 1h h h TCA Malate 1h h h Ketoglutarate 1h h h Citrate 1h h h Other Glycerol-P 1h h h Glutamate 1h h h

13 Supplementary Tables Table S1. IL-3-dependent cells were starved of glucose for 24 hours. Either 15 mm 13 C 6 -glucose, 15 mm 13 C 6 -GlcNAc, or water was added to cells. Cells were harvested and metabolites extracted at 1, 24, and 72 hours after metabolite addition. Metabolites were analyzed by LC-MS/MS. Shown are the sums of labeled + unlabeled metabolites in each sample, equal to total pool size. Table S2. IL-3-dependent cells were starved of glucose for 24 hours. Either 15 mm 13 C 6 -glucose, 15 mm 13 C 6 -GlcNAc, or water was added to cells. Cells were harvested and metabolites extracted at 1, 24, and 72 hours after metabolite addition. Metabolites were analyzed by LC-MS/MS. Shown is the total of labeled metabolites in each pool (sum of each labeled species). Note that non-zero values were obtained for some metabolites, even when no 13 C label was introduced; these readings represent background or natural abundance. 13

[U- 13 C5] glutamine. Glutamate. Acetyl-coA. Citrate. Citrate. Malate. Malate. Isocitrate OXIDATIVE METABOLISM. Oxaloacetate CO2.

[U- 13 C5] glutamine. Glutamate. Acetyl-coA. Citrate. Citrate. Malate. Malate. Isocitrate OXIDATIVE METABOLISM. Oxaloacetate CO2. Supplementary Figures a. Relative mrna levels Supplementary Figure 1 (Christofk) 3.0 2.5 2.0 1.5 1.0 0.5 0.0 LAT1 Fumarate Succinate Palmitate Acetyl-coA Oxaloacetate OXIDATIVE METABOLISM α-ketoglutarate

More information

a b c d e C 3 ]Aspartate [ 20 minutes C 3 ]Hexose-P * * *

a b c d e C 3 ]Aspartate [ 20 minutes C 3 ]Hexose-P * * * Supplemental Figure 1 Metabolic flux with [U- C]Lactate - [ 12 C]Glutamine in primary hepatocytes a b c d e [ C 3 ]Pyruvate [ C 3 ]Malate [ C 3 ]Aspartate [ C 3 ]Citrate [ C 2 ] -Ketoglutarate f g h [

More information

Integrative Metabolism: Significance

Integrative Metabolism: Significance Integrative Metabolism: Significance Energy Containing Nutrients Carbohydrates Fats Proteins Catabolism Energy Depleted End Products H 2 O NH 3 ADP + Pi NAD + NADP + FAD + Pi NADH+H + NADPH+H + FADH2 Cell

More information

Relative Rates. SUM159 CB- 839-Resistant *** n.s Intracellular % Labeled by U- 13 C-Asn 0.

Relative Rates. SUM159 CB- 839-Resistant *** n.s Intracellular % Labeled by U- 13 C-Asn 0. A Relative Growth Rates 1.2 1.8.6.4.2 B Relative Rates 1.6 1.4 1.2 1.8.6.4.2 LPS2 Parental LPS2 Q-Independent SUM159 Parental SUM159 CB-839-Resistant LPS2 Parental LPS2 Q- Independent SUM159 Parental SUM159

More information

Find this material useful? You can help our team to keep this site up and bring you even more content consider donating via the link on our site.

Find this material useful? You can help our team to keep this site up and bring you even more content consider donating via the link on our site. Find this material useful? You can help our team to keep this site up and bring you even more content consider donating via the link on our site. Still having trouble understanding the material? Check

More information

Integration of Metabolism

Integration of Metabolism Integration of Metabolism Metabolism is a continuous process. Thousands of reactions occur simultaneously in order to maintain homeostasis. It ensures a supply of fuel, to tissues at all times, in fed

More information

CARBOHYDRATE METABOLISM

CARBOHYDRATE METABOLISM Note (Study Glycolysis, fermentation and their regulation, Gluconeogenesis and glycogenolysis, Metabolism of galactose, TCA cycle and Amphibolic role of the cycle, and Glyoxalic acid cycle, HMP shunt in

More information

Physiological Chemistry II Exam IV Dr. Melissa Kelley April 13, 2004

Physiological Chemistry II Exam IV Dr. Melissa Kelley April 13, 2004 Name Write your name on the back of the exam Physiological Chemistry II Exam IV Dr. Melissa Kelley April 13, 2004 This examination consists of forty-four questions, each having 2 points. The remaining

More information

Information transmission

Information transmission 1-3-3 Case studies in Systems Biology Goutham Vemuri goutham@chalmers.se Information transmission Fluxome Metabolome flux 1 flux flux 3 Proteome metabolite1 metabolite metabolite3 protein 1 protein protein

More information

(de novo synthesis of glucose)

(de novo synthesis of glucose) Gluconeogenesis (de novo synthesis of glucose) Gluconeogenesis Gluconeogenesis is the biosynthesis of new glucose. The main purpose of gluconeogenesis is to maintain the constant blood Glc concentration.

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION SUPPLEMENTARY INFORMATION doi:10.1038/nature22359 Supplementary Discussion KRAS and regulate central carbon metabolism, including pathways supplied by abundant fuels like glucose and glutamine. Under control

More information

E.coli Core Model: Metabolic Core

E.coli Core Model: Metabolic Core 1 E.coli Core Model: Metabolic Core 2 LEARNING OBJECTIVES Each student should be able to: Describe the glycolysis pathway in the core model. Describe the TCA cycle in the core model. Explain gluconeogenesis.

More information

AMINO ACID METABOLISM. Sri Widia A Jusman Dept. of Biochemistry & Molecular Biology FMUI

AMINO ACID METABOLISM. Sri Widia A Jusman Dept. of Biochemistry & Molecular Biology FMUI AMINO ACID METABOLISM Sri Widia A Jusman Dept. of Biochemistry & Molecular Biology FMUI Amino acids derived from dietary protein absorbed from intestine through blood taken up by tissues used for biosynthesis

More information

Evidence for an Alternative Glycolytic Pathway in Rapidly Proliferating Cells. Matthew G. Vander Heiden, et al. Science 2010

Evidence for an Alternative Glycolytic Pathway in Rapidly Proliferating Cells. Matthew G. Vander Heiden, et al. Science 2010 Evidence for an Alternative Glycolytic Pathway in Rapidly Proliferating Cells Matthew G. Vander Heiden, et al. Science 2010 Introduction The Warburg Effect Cancer cells metabolize glucose differently Primarily

More information

Introduction to Carbohydrate metabolism

Introduction to Carbohydrate metabolism Introduction to Carbohydrate metabolism Some metabolic pathways of carbohydrates 1- Glycolysis 2- Krebs cycle 3- Glycogenesis 4- Glycogenolysis 5- Glyconeogenesis - Pentose Phosphate Pathway (PPP) - Curi

More information

Integration & Hormone Regulation

Integration & Hormone Regulation Integration Branchpoints in metabolism where metabolites can go several directions 1. Glucose 6-phosphate Energy needed (low energy charge): glycolysis Low blood sugar: high [glucagon], low [insulin] glycogen

More information

Control vs. HFD-lipid

Control vs. HFD-lipid Animals Control vs. T1D vs. T1D-leptin and Hyperinsulinemic-diabetic vs. hyperinsulinemic-diabetic-leptin STZ ± nicotinamide injection Leptin or saline 6 8 1 12 2 4 6 8 1 12 2 4 6 8 1 12 Control vs. HFD-lipid

More information

What is the Warburg Effect

What is the Warburg Effect What is the Warburg Effect Roles nutrients play in the biochemistry of a cell Thus, proliferating cells must acquire more nutrients, convert them into biosynthetic building blocks, and coordinate the reactions

More information

Respiration. Energy is everything!

Respiration. Energy is everything! Respiration Energy is everything! Tesla was incredible Everyone was intrigued by Tesla Tesla showed that energy does not need to be feared So what does this have to do with twinkies? Everything! Cellular

More information

Pathway overview. Glucose + 2NAD + + 2ADP +2Pi 2NADH + 2pyruvate + 2ATP + 2H 2 O + 4H +

Pathway overview. Glucose + 2NAD + + 2ADP +2Pi 2NADH + 2pyruvate + 2ATP + 2H 2 O + 4H + Glycolysis Glycolysis The conversion of glucose to pyruvate to yield 2ATP molecules 10 enzymatic steps Chemical interconversion steps Mechanisms of enzyme conversion and intermediates Energetics of conversions

More information

(A) Urea cycle (B) TCA cycle (C) Glycolysis (D) Pyruvate oxidation (E) Respiratory chain

(A) Urea cycle (B) TCA cycle (C) Glycolysis (D) Pyruvate oxidation (E) Respiratory chain Biochemistry - Problem Drill 15: Citric Acid Cycle No. 1 of 10 1. Which of the following statements is not a metabolic pathway involved in carbohydrate catabolism and ATP production. (A) Urea cycle (B)

More information

SIMPLE BASIC METABOLISM

SIMPLE BASIC METABOLISM SIMPLE BASIC METABOLISM When we eat food such as a tuna fish sandwich, the polysaccharides, lipids, and proteins are digested to smaller molecules that are absorbed into the cells of our body. As these

More information

Respiration. Energy is everything!

Respiration. Energy is everything! Respiration Energy is everything! Tesla was incredible Everyone was intrigued by Tesla Tesla showed that energy does not need to be feared So what does this have to do with twinkies? Everything! Cellular

More information

A. Generation and characterization of Ras-expressing autophagycompetent

A. Generation and characterization of Ras-expressing autophagycompetent Supplemental Material Supplemental Figure Legends Fig. S1 A. Generation and characterization of Ras-expressing autophagycompetent and -deficient cell lines. HA-tagged H-ras V12 was stably expressed in

More information

Pyruvate Alanine 0.15 *** ** ***

Pyruvate Alanine 0.15 *** ** *** SUPPLEMENTARY FIGURES Glucose ΔµM from fresh media / mg protein -1-2 -3 - -.1 -.3 -.5 Lactate Alanine Formate ΔµM from fresh media / mg protein 5 3 2 1.15.1.5.6..2.. NS-3 WT-NS G93A-NS Supplementary Figure

More information

2. What is molecular oxygen directly converted into? a. Carbon Dioxide b. Water c. Glucose d. None of the Above

2. What is molecular oxygen directly converted into? a. Carbon Dioxide b. Water c. Glucose d. None of the Above Biochem 1 Mock Exam 3 Chapter 11: 1. What is glucose completely oxidized into? a. Carbon Dioxide and Water b. Carbon Dioxide and Oxygen c. Oxygen and Water d. Water and Glycogen 2. What is molecular oxygen

More information

Comparison of catabolic and anabolic pathways

Comparison of catabolic and anabolic pathways Comparison of catabolic and anabolic pathways Three stages of catabolism Glucose Synthesis of compounds e.g. lactose glycolipids Glucose-6-P Pentosephosphate Pathway Glycolysis Glycogenesis Acetyl-CoA

More information

Fate of Dietary Protein

Fate of Dietary Protein Fate of Dietary Protein Dietary protein Stomach: l, pepsin Denatured and partially hydrolyzed protein (large polypeptides) small intestine: proteases Amino acids and dipeptides intestinal lining: proteases

More information

Amino Acid Metabolism

Amino Acid Metabolism Amino Acid Metabolism Fate of Dietary Protein Dietary protein Stomach: l, pepsin Denatured and partially hydrolyzed protein (large polypeptides) small intestine: proteases Amino acids and dipeptides intestinal

More information

Review of Carbohydrate Digestion

Review of Carbohydrate Digestion Review of Carbohydrate Digestion Glycolysis Glycolysis is a nine step biochemical pathway that oxidizes glucose into two molecules of pyruvic acid. During this process, energy is released and some of it

More information

Glycogen Metabolism. BCH 340 lecture 9

Glycogen Metabolism. BCH 340 lecture 9 Glycogen Metabolism BC 340 lecture 9 Structure of glycogen Glycogen is homopolysaccharide formed of branched D-glucose units The primary glycosidic bond is 1-4-linkage Each branch is made of 6-12 glucose

More information

Yves Gibon INRA Bordeaux

Yves Gibon INRA Bordeaux Metabolic phenotyping? Assay principles & technology Examples of application Enzyme profiles in pine needles Major metabolites across a Maize NAM population Yves Gibon INRA Bordeaux Primary metabolic

More information

Case Study: Carbohydrate Metabolism. eating an early dinner the night before and skipping breakfast that morning, Sid goes to the

Case Study: Carbohydrate Metabolism. eating an early dinner the night before and skipping breakfast that morning, Sid goes to the Student Name Biochemistry 4320 Case Study Part I 4 November 2013 Case Study: Carbohydrate Metabolism Sid is a high school student who has decided to start exercising before school. After eating an early

More information

doi: /nature10642

doi: /nature10642 doi:10.1038/nature10642 Supplementary Fig. 1. Citric acid cycle (CAC) metabolism in WT 143B and CYTB 143B cells. a, Proliferation of WT 143B and CYTB 143B cells. Doubling times were 28±1 and 33±2 hrs for

More information

Chapter 22. Before the class. 10 Steps of glycolysis. Outline. Can you tell the ten steps of glycolysis? Do you know how glucoses are

Chapter 22. Before the class. 10 Steps of glycolysis. Outline. Can you tell the ten steps of glycolysis? Do you know how glucoses are Chapter 22 Gluconeogenesis, Glycogen metabolism, and the Pentose Phosphate Pathway Reginald H. Garrett Charles M. Grisham 1 Before the class Can you tell the ten steps of glycolysis? Do you know how glucoses

More information

III. 6. Test. Respiració cel lular

III. 6. Test. Respiració cel lular III. 6. Test. Respiració cel lular Chapter Questions 1) What is the term for metabolic pathways that release stored energy by breaking down complex molecules? A) anabolic pathways B) catabolic pathways

More information

Lecture: Amino Acid catabolism: Nitrogen-The Urea cycle

Lecture: Amino Acid catabolism: Nitrogen-The Urea cycle BIOC 423: Introductory Biochemistry Biochemistry Education Department of Biochemistry & Molecular Biology University of New Mexico Lecture: Amino Acid catabolism: Nitrogen-The Urea cycle OBJECTIVES Describe

More information

4. Which step shows a split of one molecule into two smaller molecules? a. 2. d. 5

4. Which step shows a split of one molecule into two smaller molecules? a. 2. d. 5 1. Which of the following statements about NAD + is false? a. NAD + is reduced to NADH during both glycolysis and the citric acid cycle. b. NAD + has more chemical energy than NADH. c. NAD + is reduced

More information

: -CH2O - anadph + bproduct + catp + dnadh + eco2 2O + 2NADPH + CO 2 2O ATP + CO 2

: -CH2O - anadph + bproduct + catp + dnadh + eco2 2O + 2NADPH + CO 2 2O ATP + CO 2 Supplementary Notes In the calculations below metabolism is assumed to be respiro-fermentative in nature, which is generally the case under high glycolytic flux and is the best case scenario for pathways

More information

Midterm 2. Low: 14 Mean: 61.3 High: 98. Standard Deviation: 17.7

Midterm 2. Low: 14 Mean: 61.3 High: 98. Standard Deviation: 17.7 Midterm 2 Low: 14 Mean: 61.3 High: 98 Standard Deviation: 17.7 Lecture 17 Amino Acid Metabolism Review of Urea Cycle N and S assimilation Last cofactors: THF and SAM Synthesis of few amino acids Dietary

More information

Cellular Respiration Other Metabolites & Control of Respiration. AP Biology

Cellular Respiration Other Metabolites & Control of Respiration. AP Biology Cellular Respiration Other Metabolites & Control of Respiration Cellular respiration: Beyond glucose: Other carbohydrates: Glycolysis accepts a wide range of carbohydrates fuels. polysaccharides glucose

More information

METABOLISM Sri Widia A Jusman Department of Biochemistry & Molecular Biology FMUI

METABOLISM Sri Widia A Jusman Department of Biochemistry & Molecular Biology FMUI METABOLISM Sri Widia A Jusman Department of Biochemistry & Molecular Biology FMUI 1 METABOLISM Process of how cells acquire, transform, store and use energy Study of the chemistry, regulation and energetics

More information

Loss of protein association causes cardiolipin degradation in Barth syndrome

Loss of protein association causes cardiolipin degradation in Barth syndrome SUPPLEMENTARY INFORMATION Loss of protein association causes cardiolipin degradation in Barth syndrome Yang Xu 1, Colin K.L. Phoon 2, Bob Berno 5, Kenneth D Souza 6, Esthelle Hoedt 4, Guoan Zhang 4, Thomas

More information

Krebs cycle Energy Petr Tůma Eva Samcová

Krebs cycle Energy Petr Tůma Eva Samcová Krebs cycle Energy - 215 Petr Tůma Eva Samcová Overview of Citric Acid Cycle Key Concepts The citric acid cycle (Krebs cycle) is a multistep catalytic process that converts acetyl groups derived from carbohydrates,

More information

Biochemistry: A Short Course

Biochemistry: A Short Course Tymoczko Berg Stryer Biochemistry: A Short Course First Edition CHAPTER 19 Harvesting Electrons from the Cycle 2013 W. H. Freeman and Company Chapter 19 Outline The citric acid cycle oxidizes the acetyl

More information

CH395G FINAL (3 rd ) EXAM Kitto/Hackert - Fall 2003

CH395G FINAL (3 rd ) EXAM Kitto/Hackert - Fall 2003 CH395G FINAL (3 rd ) EXAM Kitto/Hackert - Fall 2003 1. A cell in an active, catabolic state has a. a high (ATP/ADP) and a high (NADH/NAD + ) ratio b. a high (ATP/ADP) and a low (NADH/NAD + ) ratio c. a

More information

New tools bring greater understanding to cellular metabolism research

New tools bring greater understanding to cellular metabolism research New tools bring greater understanding to cellular metabolism research Mourad Ferhat, Ph.D, 7 Juin 2017 FDSS Users Meeting, Hamamatsu mourad.ferhat@promega.com Today s talk : focus on new cell-based assays

More information

Energy metabolism - the overview

Energy metabolism - the overview Energy metabolism - the overview Josef Fontana EC - 40 Overview of the lecture Important terms of the energy metabolism The overview of the energy metabolism The main pathways of the energy metabolism

More information

Inhibition of fatty acid oxidation as a therapy for MYC-overexpressing triplenegative

Inhibition of fatty acid oxidation as a therapy for MYC-overexpressing triplenegative SUPPLEMENTARY INFORMATION Inhibition of fatty acid oxidation as a therapy for MYC-overexpressing triplenegative breast cancer Roman Camarda, Alicia Y. Zhou, Rebecca A. Kohnz, Sanjeev Balakrishnan, Celine

More information

GLYCOLYSIS Generation of ATP from Metabolic Fuels

GLYCOLYSIS Generation of ATP from Metabolic Fuels GLYCOLYSIS Generation of ATP from Metabolic Fuels - Catabolic process degradative pathway - Energy stored in sugars (carbohydrates) released to perform biological work - Transforms GLUCOSE to PYRUVATE

More information

How do we retain emphasis on function?

How do we retain emphasis on function? Systems Biology Systems biology studies biological systems by systematically perturbing them (biologically, genetically, or chemically); monitoring the gene, protein, and informational pathway responses;

More information

Biochemistry - Problem Drill 16: Carbohydrate Metabolism

Biochemistry - Problem Drill 16: Carbohydrate Metabolism Biochemistry - Problem Drill 16: Carbohydrate Metabolism No. 1 of 10 Instructions: (1) Read the problem statement and answer choices carefully (2) Work the problems on paper as 1. Fill in the diagram below

More information

This is an example outline of 3 lectures in BSC (Thanks to Dr. Ellington for sharing this information.)

This is an example outline of 3 lectures in BSC (Thanks to Dr. Ellington for sharing this information.) This is an example outline of 3 lectures in BSC 2010. (Thanks to Dr. Ellington for sharing this information.) Topic 10: CELLULAR RESPIRATION (lectures 14-16) OBJECTIVES: 1. Know the basic reactions that

More information

glucose glucose 6-phospho fructose 6-phosphate fructose 1,6-bisphosphate glyceraldehyde 3-phosphate 1,3-bisphosphoglycerate 3-phosphoglycerate

glucose glucose 6-phospho fructose 6-phosphate fructose 1,6-bisphosphate glyceraldehyde 3-phosphate 1,3-bisphosphoglycerate 3-phosphoglycerate Cells glucose glucose glucose 6-phospho glycogen pentose phosphate T 1-phosphate 6-phosphate gluconate CC T CC+PD-1 pathway Isobar: fructose 1,6-diphosphate; glucose 1,6-diphosphate DHAP lactate fructose

More information

Journal of Cell Science Supplementary information. Arl8b +/- Arl8b -/- Inset B. electron density. genotype

Journal of Cell Science Supplementary information. Arl8b +/- Arl8b -/- Inset B. electron density. genotype J. Cell Sci. : doi:.4/jcs.59: Supplementary information E9. A Arl8b /- Arl8b -/- Arl8b Arl8b non-specific band Gapdh Tbp E7.5 HE Inset B D Control al am hf C E Arl8b -/- al am hf E8.5 F low middle high

More information

Biochemistry 423 Final Examination NAME:

Biochemistry 423 Final Examination NAME: Biochemistry 423 Final Examination NAME: 1 Circle the single BEST answer (3 points each) 1. At equilibrium the free energy of a reaction G A. depends only on the temperature B. is positive C. is 0 D. is

More information

Lecture 5: Cell Metabolism. Biology 219 Dr. Adam Ross

Lecture 5: Cell Metabolism. Biology 219 Dr. Adam Ross Lecture 5: Cell Metabolism Biology 219 Dr. Adam Ross Cellular Respiration Set of reactions that take place during the conversion of nutrients into ATP Intricate regulatory relationship between several

More information

University of Palestine. Final Exam 2016/2017 Total Grade:

University of Palestine. Final Exam 2016/2017 Total Grade: Part 1 : Multiple Choice Questions (MCQs) 1)Which of the following statements about Michaelis-Menten kinetics is correct? a)k m, the Michaelis constant, is defined as the concentration of substrate required

More information

Welcome to Class 14! Class 14: Outline and Objectives. Overview of amino acid catabolism! Introductory Biochemistry!

Welcome to Class 14! Class 14: Outline and Objectives. Overview of amino acid catabolism! Introductory Biochemistry! Welcome to Class 14 Introductory Biochemistry Class 14: Outline and Objectives Amino Acid Catabolism Fates of amino groups transamination urea cycle Fates of carbon skeletons important cofactors metabolic

More information

Integration Of Metabolism

Integration Of Metabolism Integration Of Metabolism Metabolism Consist of Highly Interconnected Pathways The basic strategy of catabolic metabolism is to form ATP, NADPH, and building blocks for biosyntheses. 1. ATP is the universal

More information

ANSC/NUTR 618 Lipids & Lipid Metabolism

ANSC/NUTR 618 Lipids & Lipid Metabolism I. verall concepts A. Definitions ANSC/NUTR 618 Lipids & Lipid Metabolism 1. De novo synthesis = synthesis from non-fatty acid precursors a. Carbohydrate precursors (glucose and lactate) 1) Uses glucose

More information

CHY2026: General Biochemistry UNIT 7& 8: CARBOHYDRATE METABOLISM

CHY2026: General Biochemistry UNIT 7& 8: CARBOHYDRATE METABOLISM CHY2026: General Biochemistry UNIT 7& 8: CARBOHYDRATE METABOLISM Metabolism Bioenergetics is the transfer and utilization of energy in biological systems The direction and extent to which a chemical reaction

More information

Chemistry 3503 Final exam April 17, Student s name:

Chemistry 3503 Final exam April 17, Student s name: Chemistry 3503 Final exam April 17, 2008 Student s name: THIS EXAM IS FOR STUDENTS IN D. CRAIG S SECTION. IF YOU ARE IN M. EZE S SECTION THIS EXAM IS NOT FOR YOU. Part I /40 Part II Question 1 /4 Question

More information

BY: RASAQ NURUDEEN OLAJIDE

BY: RASAQ NURUDEEN OLAJIDE BY: RASAQ NURUDEEN OLAJIDE LECTURE CONTENT INTRODUCTION CITRIC ACID CYCLE (T.C.A) PRODUCTION OF ACETYL CoA REACTIONS OF THE CITIRC ACID CYCLE THE AMPHIBOLIC NATURE OF THE T.C.A CYCLE THE GLYOXYLATE CYCLE

More information

INTRODUCTORY BIOCHEMISTRY. BI 28 Second Midterm Examination April 3, 2007

INTRODUCTORY BIOCHEMISTRY. BI 28 Second Midterm Examination April 3, 2007 INTRODUCTORY BIOCHEMISTRY BI 28 Second Midterm Examination April 3, 2007 Name SIS # Make sure that your name or SIS # is on every page. This is the only way we have of matching you with your exam after

More information

TCA CYCLE (Citric Acid Cycle)

TCA CYCLE (Citric Acid Cycle) TCA CYCLE (Citric Acid Cycle) TCA CYCLE The Citric Acid Cycle is also known as: Kreb s cycle Sir Hans Krebs Nobel prize, 1953 TCA (tricarboxylic acid) cycle The citric acid cycle requires aerobic conditions!!!!

More information

Nitrogen Metabolism. Pratt and Cornely Chapter 18

Nitrogen Metabolism. Pratt and Cornely Chapter 18 Nitrogen Metabolism Pratt and Cornely Chapter 18 Overview Nitrogen assimilation Amino acid biosynthesis Nonessential aa Essential aa Nucleotide biosynthesis Amino Acid Catabolism Urea Cycle Juicy Steak

More information

ANSC/NUTR 618 Lipids & Lipid Metabolism

ANSC/NUTR 618 Lipids & Lipid Metabolism Fatty Acid ynthesis I. verall concepts A. Definitions ANC/NUTR 618 Lipids & Lipid Metabolism Fatty Acid ynthesis 1. De novo synthesis = synthesis from non-fatty acid precursors a. Carbohydrate precursors

More information

In glycolysis, glucose is converted to pyruvate. If the pyruvate is reduced to lactate, the pathway does not require O 2 and is called anaerobic

In glycolysis, glucose is converted to pyruvate. If the pyruvate is reduced to lactate, the pathway does not require O 2 and is called anaerobic Glycolysis 1 In glycolysis, glucose is converted to pyruvate. If the pyruvate is reduced to lactate, the pathway does not require O 2 and is called anaerobic glycolysis. If this pyruvate is converted instead

More information

SCBC203 Amino Acid Metabolism

SCBC203 Amino Acid Metabolism Breakdown of proteins Route I: Dietary protein breakdown SCBC203 Amino Acid Metabolism Dr Sarawut Jitrapakdee Professor of Biochemistry Department of Biochemistry Faculty of Science Mahidol University

More information

Nature Genetics: doi: /ng Supplementary Figure 1. HOX fusions enhance self-renewal capacity.

Nature Genetics: doi: /ng Supplementary Figure 1. HOX fusions enhance self-renewal capacity. Supplementary Figure 1 HOX fusions enhance self-renewal capacity. Mouse bone marrow was transduced with a retrovirus carrying one of three HOX fusion genes or the empty mcherry reporter construct as described

More information

Tutorial 27: Metabolism, Krebs Cycle and the Electron Transport Chain

Tutorial 27: Metabolism, Krebs Cycle and the Electron Transport Chain Tutorial 27: Metabolism, Krebs Cycle and the Electron Transport Chain Goals: To be able to describe the overall catabolic pathways for food molecules. To understand what bonds are hydrolyzed in the digestion

More information

Intermediary metabolism. Eva Samcová

Intermediary metabolism. Eva Samcová Intermediary metabolism Eva Samcová Metabolic roles of tissues Four major tissues play a dominant role in fuel metabolism : liver, adipose, muscle, and brain. These tissues do not function in isolation.

More information

Moh Tarek. Razi Kittaneh. Jaqen H ghar

Moh Tarek. Razi Kittaneh. Jaqen H ghar 14 Moh Tarek Razi Kittaneh Jaqen H ghar Naif Karadsheh Gluconeogenesis is making glucose from non-carbohydrates precursors. Although Gluconeogenesis looks like Glycolysis in many steps, it is not the simple

More information

Human recombinat MIF protein (hrmif), MW: Da. m/z. hrmif ( Da) + 4-IPP (282 Da) MWtot ~ Da. m/z.

Human recombinat MIF protein (hrmif), MW: Da. m/z. hrmif ( Da) + 4-IPP (282 Da) MWtot ~ Da. m/z. Intensity % Intensity % A Human recombinat MIF protein (hrmif), MW: 12428.31 Da m/z hrmif (12428.31 Da) + 4-IPP (282 Da) MWtot ~ 12715.21 Da m/z B HTC/C3 DAPI phistone-h3 Merge HTC/C3 DAPI phistone-h3

More information

Nitrogen Metabolism. Overview

Nitrogen Metabolism. Overview Nitrogen Metabolism Pratt and Cornely Chapter 18 Overview Nitrogen assimilation Amino acid biosynthesis Nonessential aa Essential aa Nucleotide biosynthesis Amino Acid Catabolism Urea Cycle Juicy Steak

More information

respiration mitochondria mitochondria metabolic pathways reproduction can fuse or split DRP1 interacts with ER tubules chapter DRP1 ER tubule

respiration mitochondria mitochondria metabolic pathways reproduction can fuse or split DRP1 interacts with ER tubules chapter DRP1 ER tubule mitochondria respiration chapter 3-4 shape highly variable can fuse or split structure outer membrane inner membrane cristae intermembrane space mitochondrial matrix free ribosomes respiratory enzymes

More information

Nitrogen Metabolism. Overview

Nitrogen Metabolism. Overview Nitrogen Metabolism Pratt and Cornely Chapter 18 Overview Nitrogen assimilation Amino acid biosynthesis Nonessential aa Essential aa Nucleotide biosynthesis Amino Acid Catabolism Urea Cycle Juicy Steak

More information

Citric Acid Cycle: Central Role in Catabolism. Entry of Pyruvate into the TCA cycle

Citric Acid Cycle: Central Role in Catabolism. Entry of Pyruvate into the TCA cycle Citric Acid Cycle: Central Role in Catabolism Stage II of catabolism involves the conversion of carbohydrates, fats and aminoacids into acetylcoa In aerobic organisms, citric acid cycle makes up the final

More information

Glycolysis. Intracellular location Rate limiting steps

Glycolysis. Intracellular location Rate limiting steps Glycolysis Definition Fx Fate Site Intracellular location Rate limiting steps Regulation Consume ATP Subs level phosphoryla tion Key reactions control points Nb Oxidation of glucose to give pyruvate (

More information

Marah Bitar. Faisal Nimri ... Nafeth Abu Tarboosh

Marah Bitar. Faisal Nimri ... Nafeth Abu Tarboosh 8 Marah Bitar Faisal Nimri... Nafeth Abu Tarboosh Summary of the 8 steps of citric acid cycle Step 1. Acetyl CoA joins with a four-carbon molecule, oxaloacetate, releasing the CoA group and forming a six-carbon

More information

Figure S1A. Blood glucose levels in mice after glucose injection

Figure S1A. Blood glucose levels in mice after glucose injection ## Figure S1A. Blood glucose levels in mice after glucose injection Blood glucose (mm/l) 25 2 15 1 5 # 15 3 6 3+3 Time after glucose injection (min) # Figure S1B. α-kg levels in mouse livers after glucose

More information

Metabolism III. Aim: understand gluconeogenesis, pentose phosphate pathway, photosynthesis and amino acid synthesis

Metabolism III. Aim: understand gluconeogenesis, pentose phosphate pathway, photosynthesis and amino acid synthesis Metabolism III Aim: understand gluconeogenesis, pentose phosphate pathway, photosynthesis and amino acid synthesis Anabolism From a carbon source and inorganic molecules, microbes synthesize new organelles

More information

CELLULAR RESPIRATION SUMMARY EQUATION. C 6 H 12 O 6 + O 2 6CO2 + 6H 2 O + energy (ATP) STEPWISE REDOX REACTION

CELLULAR RESPIRATION SUMMARY EQUATION. C 6 H 12 O 6 + O 2 6CO2 + 6H 2 O + energy (ATP) STEPWISE REDOX REACTION CELLULAR RESPIRATION SUMMARY EQUATION C 6 H 12 O 6 + O 2 6CO2 + 6H 2 O + energy (ATP) STEPWISE REDOX REACTION Oxidation: partial or complete loss of electrons Reduction: partial or complete gain of electrons

More information

Integration of Metabolism 1. made by: Noor M. ALnairat. Sheet No. 18

Integration of Metabolism 1. made by: Noor M. ALnairat. Sheet No. 18 Integration of Metabolism 1 made by: Noor M. ALnairat Sheet No. 18 Data :24/11/2016 SLIDE 2: Metabolism Consist of Highly Interconnected Pathways The basic strategy of catabolic metabolism is to form ATP,

More information

Medical Biochemistry and Molecular Biology department

Medical Biochemistry and Molecular Biology department Medical Biochemistry and Molecular Biology department Cardiac Fuels [Sources of energy for the Cardiac muscle] Intended learning outcomes of the lecture: By the end of this lecture you would be able to:-

More information

METABOLISM Biosynthetic Pathways

METABOLISM Biosynthetic Pathways METABOLISM Biosynthetic Pathways Metabolism Metabolism involves : Catabolic reactions that break down large, complex molecules to provide energy and smaller molecules. Anabolic reactions that use ATP energy

More information

Unit 2: Metabolic Processes

Unit 2: Metabolic Processes How is energy obtained biologically? Recall: Red Ox Reactions Unit 2: Metabolic Processes Oxidation Is the chief mechanism by which chemical potential energy is released This energy comes from reduced

More information

Chapter 15 Glycolysis and The Pentose Phosphate Pathway

Chapter 15 Glycolysis and The Pentose Phosphate Pathway Principles of Biochemistry Fourth Edition Donald Voet Judith G. Voet harlotte W. Pratt hapter 15 Glycolysis and The Pentose Phosphate Pathway Page No. 47-490 Introduction Glucose: is major source of metabolic

More information

Discussion of Prism modules and predicted interactions (Fig. 4)

Discussion of Prism modules and predicted interactions (Fig. 4) SUPPLEMENTARY NOTES Discussion of Prism modules and predicted interactions (Fig. 4) a. Interactions of the TCA-cycle, respiratory chain, and ATP synthetase with the amino acid biosynthesis modules. Given

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION In the format provided by the authors and unedited. SUPPLEMENTARY INFORMATION VOLUME: 2 ARTICLE NUMBER: 17084 Metabolic anticipation in Mycobacterium tuberculosis Hyungjin Eoh, Zhe Wang, Emilie Layre,

More information

Module No. # 01 Lecture No. # 19 TCA Cycle

Module No. # 01 Lecture No. # 19 TCA Cycle Biochemical Engineering Prof. Dr. Rintu Banerjee Department of Agricultural and Food Engineering Asst. Prof. Dr. Saikat Chakraborty Department of Chemical Engineering Indian Institute of Technology, Kharagpur

More information

Carbohydrate. Metabolism

Carbohydrate. Metabolism Carbohydrate Metabolism Dietary carbohydrates (starch, glycogen, sucrose, lactose Mouth salivary amylase Summary of Carbohydrate Utilization Utilization for energy (glycolysis) ligosaccharides and disaccharides

More information

number Done by Corrected by Doctor Nayef Karadsheh

number Done by Corrected by Doctor Nayef Karadsheh number 13 Done by Asma Karameh Corrected by Saad hayek Doctor Nayef Karadsheh Gluconeogenesis This lecture covers gluconeogenesis with aspects of: 1) Introduction to glucose distribution through tissues.

More information

Chapter 17 - Citric Acid Cycle

Chapter 17 - Citric Acid Cycle hapter 17 - itric Acid ycle I. Introduction - The citric acid cycle (A) was elucidated in the 1930's by ans Krebs, who first noticed that oxygen consumption in suspensions of pigeon breast muscle was greatly

More information

Metabolism of amino acids. Vladimíra Kvasnicová

Metabolism of amino acids. Vladimíra Kvasnicová Metabolism of amino acids Vladimíra Kvasnicová Classification of proteinogenic AAs -metabolic point of view 1) biosynthesis in a human body nonessential (are synthesized) essential (must be present in

More information

Modifications of Pyruvate Handling in Health and Disease Prof. Mary Sugden

Modifications of Pyruvate Handling in Health and Disease Prof. Mary Sugden Modifications of Handling Modifications of Handling Centre for Diabetes and Metabolic Medicine Institute of Cell and Molecular Science Barts and the London School of Medicine and Dentistry 1 Potential

More information

How Cells Harvest Chemical Energy

How Cells Harvest Chemical Energy Chapter 6 How Cells Harvest Chemical Energy INTRODUCTION TO CELLULAR RESIRATION hotosynthesis and cellular respiration provide energy for life Cellular respiration makes and consumes O During the oxidation

More information

Supplementary Figure 1. PD-L1 is glycosylated in cancer cells. (a) Western blot analysis of PD-L1 in breast cancer cells. (b) Western blot analysis

Supplementary Figure 1. PD-L1 is glycosylated in cancer cells. (a) Western blot analysis of PD-L1 in breast cancer cells. (b) Western blot analysis Supplementary Figure 1. PD-L1 is glycosylated in cancer cells. (a) Western blot analysis of PD-L1 in breast cancer cells. (b) Western blot analysis of PD-L1 in ovarian cancer cells. (c) Western blot analysis

More information

Class XI Chapter 14 Respiration in Plants Biology. 1. It is a biochemical process. 1. It is a physiochemical process.

Class XI Chapter 14 Respiration in Plants Biology. 1. It is a biochemical process. 1. It is a physiochemical process. Question 1: Differentiate between (a) Respiration and Combustion (b) Glycolysis and Krebs cycle (c) Aerobic respiration and Fermentation (a) Respiration and combustion Respiration Combustion 1. It is a

More information