CELL CULTURE MEDIA AND REAGENTS

Transcription

1 CELL CULTURE MEDIA AND REAGENTS Classical Media Buffered Salt Solutions Reagents & Supplements

2 CELL CULTURE MEDIA AND REAGENTS MEDIA DULBECCO S MODIFIED EAGLE S MEDIUM (DMEM) A modification of Basal Medium Eagle (BME), Dulbecco s Modified Eagle's Medium (DMEM), that contains approximately four times as much vitamins and amino acids and two to four times as much glucose as the original formulation. DMEM is suitable for most types of cells, including human, monkey, hamster, rat, and mouse. DMEM is available in a variety of formulations including high or low glucose, with or without sodium pyruvate, and with or without L-glutamine. Description DMEM 4.5 g/l w/ Sodium Pyruvate and DMEM 4.5 g/l w/ Sodium Pyruvate, w/out DMEM 4.5 g/l w/out Sodium Pyruvate, w/ DMEM 4.5 g/l w/out Sodium Pyruvate and DMEM 1.0 g/l w/ Sodium Pyruvate and DMEM 1.0 g/l w/ Sodium Pyruvate, w/out Cat. No. VWRL VWRL VWRL VWRL VWRL VWRL Components mg/l mg/l mg/l mg/l mg/l mg/l Glycine L-Arginine HCl L-Cystine 2HCl L-Histidine HCl H 2 O L-Isoleucine AMINO ACIDS INORGANIC SALTS VITAMINS OTHER L-Leucine L-Lysine HCl L-Methionine L-Phenylalanine L-Serine L-Threonine L-Tryptophan L-Tyrosine 2Na 2H 2 O L-Valine CaCl2 (Anhydrous) Fe(NO 3 ) 9H KCl MgSO 4 7H 2 O NaHCO NaCl NaH 2 PO Choline Chloride D-Calcium Pantothenate Folic Acid i-inositol Niacinamide Pyridoxine HCl Riboflavin Thiamine HCl D-Glucose (Dextrose) Phenol Red Sodium Pyruvate DMEM, 4.5 g/l Glucose w/ Sodium Pyruvate and 500 VWRL Each w/ Sodium Pyruvate, w/out 500 VWRL Each w/out Sodium Pyruvate, w/ 500 VWRL Each w/out Sodium Pyruvate and 500 VWRL Each DMEM, 1.0 g/l Glucose w/ Sodium Pyruvate and 500 VWRL Each w/ Sodium Pyruvate, w/out 500 VWRL Each 2 VWR CELL CULTURE MEDIA AND REAGENTS PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL

3 RPMI-1640 RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth in many types of cultured cells. AMINO ACIDS INORGANIC SALTS VITAMINS OTHER Description RPMI 1640 w/ RPMI 1640 w/out Cat. No. VWRL VWRL Components mg/l mg/l Glycine L-Arginine L-Asparagine L-Aspartic Acid L-Cystine 2HCl L-Glutamic Acid L-Histidine L-Hydroxyproline L-Isoleucine L-Leucine L-Lysine HCl L-Methionine L-Phenylalanine L-Proline L-Serine L-Threonine L-Tryptophan L-Tyrosine 2Na 2H 2 O L-Valine Ca(NO 3 ) 2 4H 2 O MgSO 4 7H 2 O KCl NaHCO NaCl Na 2 HPO 4 7H 2 O Biotin Choline Chloride D-Calcium Pantothenate Folic Acid i-inositol Niacinamide Para-Aminobenzoic Acid Pyridoxine HCl Riboflavin Thiamine HCl Vitamin B D-Glucose (Dextrose) Glutathione (Reduced) Phenol Red RPMI 1640 w/ 500 VWRL Each RPMI 1640 w/out 500 VWRL Each PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL VWR CELL CULTURE MEDIA AND REAGENTS 3

4 CELL CULTURE MEDIA AND REAGENTS BUFFERED SALT SOLUTIONS DULBECCO S PHOSPHATE BUFFERED SALINE (DPBS) Dulbecco s Phosphate Buffered Saline (DPBS) is a balanced salt solution used for the handling and culturing of mammalian cells. Phosphate buffering maintains the ph in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. INORGANIC SALTS Description DPBS (1X) w/ Ca and Mg DPBS (1X) w/out Ca and Mg Cat. No. VWRL , VWRL Components g/l g/l CaCl 2 (Anhdyrous) MgCl 2 6H 2 O NaCl 8 8 KCl Na 2 HPO 4 7H 2 O KH 2 PO VWRL , VWRL DPBS (1X) w/ Ca and Mg 500 VWRL Each DPBS (1X) w/ Ca and Mg 1000 VWRL Each DPBS (1X) w/out Ca and Mg 500 VWRL Each DPBS (1X) w/out Ca and Mg 1000 VWRL Each HANK S BALANCED SALT SOLUTION (HBSS) Hank s Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological ph for cells maintained in non-co 2 atmospheric conditions. HBSS can be used for washing cells or as a solution for transporting cells and tissue culture. INORGANIC SALTS HBSS (1X) w/out Ca, Mg, and Phenol Red VWRL Components g/l % of Solution NaCl 8 0.8% KCl % Na 2 HPO 4 (Anhydrous) % KH 2 PO 4 (Monobasic) % NaHCO % OTHER D-Glucose (Dextrose) 1 0.1% HBSS (1X) w/out Ca, Mg, and Phenol Red 500 VWRL Each 4 VWR CELL CULTURE MEDIA AND REAGENTS PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL

5 WATER, STERILE, LOW ENDOTOXIN, TISSUE CULTURE GRADE Water, sterile, low endotoxin, cell culture grade is ideal for use in the preparation of cell culture media and reagents. Test ph Sterility Total Organic Carbon (Before Packaging) Conductivity (Before Packaging) Endotoxin Check and Record Negative NMT 500 ppb NMT 1.3 µs/cm NMT 0.25 EU/mL Other Storage Conditions 15 C to 30 C For Further Manufacturing or Research Use. Not for Therapeutic Applications. Size Cat. No. Unit 500 ml VWRL Each 1 L VWRL Each AMINO ACIDS Amino acids supply nutrients to cells in culture supporting growth. Charged Amino Acids L-Arginine 100 g Each L-Arginine 500 g Each L-Aspartic Acid 500 g Each L-Aspartic Acid 1 kg Each L-Glutamic Acid 1 kg Each L-Glutamic Acid 2.5 kg Each L-Histidine 25 g Each L-Histidine 100 g Each L-Histidine 100 g Each L-Histidine 500 g Each L-Histidine 500 g Each L-Histidine 1 kg Each L-Lysine 100 g Each L-Lysine 500 g Each Hydrophobic Amino Acids L-Alanine 100 g Each L-Alanine 250 g Each L-Alanine 500 g Each L-Glycine, Biotechnology Grade 1 kg Each L-Glycine, Proteomics Grade 1 kg Each L-Glycine, Biotechnology Grade 5 kg Each L-Glycine, Proteomics Grade 5 kg Each L-Glycine 10 kg Each Hydrophobic Amino Acids L-Glycine 12 kg Each L-Glycine 50 kg Each L-Isoleucine 25 g Each L-Isoleucine 100 g Each L-Leucine 25 g Each L-Leucine 100 g Each L-Leucine 250 g Each L-Methionine 25 g Each L-Methionine 100 g Each L-Methionine 250 g Each L-Phenyalanine 25 g Each L-Phenyalanine 100 g Each L-Phenyalanine 500 g Each L-Proline 25 g Each L-Proline 100 g Each L-Proline 500 g Each L-Typtophan 25 g Each L-Typtophan 100 g Each L-Typtophan 500 g Each L-Valine 25 g Each L-Valine 100 g Each L-Valine 500 g Each PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL VWR CELL CULTURE MEDIA AND REAGENTS 5

6 CELL CULTURE MEDIA AND REAGENTS REAGENTS AND SUPPLEMENTS ANTIBIOTICS Antibiotics are used to prevent contamination in cell culture. The use of antibiotics in cell culture is dependent on the needs of the researcher. Ampicillin, Gamma Irradiated, Tissue Culture 20 ml Each Grade Ampicillin, Sodium Salt, USP Grade 20 g Each Ampicillin, Sodium Salt, USP Grade 100 g Each Ampicillin, Trihydrate, USP Grade 50 g Each Ampicillin, Trihydrate, USP Grade 100 g Each Ampicillin, Trihydrate, USP Grade 500 g Each Carbenicillin, Disodium Salt, Ultra Pure Grade 250 mg Each Carbenicillin, Disodium Salt, Ultra Pure Grade 1 g Each Penicillin G, Sodium Salt, USP Grade 100 MU Each Penicillin G, Sodium Salt, USP Grade 1 BU Each Penicillin G, Sodium Salt, Irradiated, Tissue 2 ml Each Culture Grade Penicillin Streptomycin, Irradiated, Tissue Culture Grade 20 ml Each CITRIC ACID, TRISODIUM SALT, DIHYDRATE A biological buffer used for cell culture, in vitro, enzyme assays and some electrophoretic applications at physiological ph. Size Cat. No. Unit 500 g Each 1 kg Each 2.5 kg Each 12 kg Each 50 kg Each DIMETHYL SULFOXIDE, ULTRAPURE (DMSO) Used in cell culture applications >99.9% pure Sterile tested Dimethyl Sulfoxide, Ultrapure 5 x 10 ml Each L-GLUTAMINE L-glutamine is an essential amino acid. It is used as an energy source for cells in cell culture. L-glutamine is stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions. Components, 200mM (100X) VWRL g/l 29.2 NaCl 8.5, 200mM (100X) 100 VWRL Each GLYCEROL, STERILE, BIOTECHNOLOGY GRADE Cryoprotectant used in cell freezing Glycerol, Sterile, Biotechnology Grade 100 ml Each SERAFREE CRYOPRESERVATION MEDIA Sterile and endotoxin tested Reduces potential for transmission of infectious agents Eliminates regulatory compliance requirements associated with use of animal products Cost effective SeraFree Cryopreservation Media are ready-to-use freezing media for cryopreservation of adherent or suspension cultured cells. The animal-free RPMI- or DMEMbased media composition eliminates batch-to-batch variability and is optimized for cell viability and cell growth after thawing. Serafree Cryopreservation Media for DMEM 50 ml Each Serafree Cryopreservation Media for RPMI 6 x 5 ml Each Serafree Cryopreservation Media for RPMI 50 ml Each SODIUM CHLORIDE Sodium chloride is a common source of salts for media propagation of many organisms. Size Cat. No. Unit 500 g Each 1 kg Each 2.5 kg Each 5 kg Each 10 kg Each 12 kg Each 50 kg Each 100 kg Each 6 VWR CELL CULTURE MEDIA AND REAGENTS PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL

7 SUGARS High purity sugars are used for media supplements and are a primary source of energy for cells in culture. D-Fructose 1 kg Each D-Fructose 2.5 kg Each D-Fructose 5 kg Each D-Fructose 12 kg Each D-(+)-Galactose 100 g Each D-(+)-Galactose 250 g Each D-(+)-Galactose 500 g Each D- Anhydrous 500 g Each D- Anhydrous 1 kg Each D- Anhydrous 2.5 kg Each D- Anhydrous 5 kg Each D- Anhydrous 12 kg Each D- Anhydrous 50 kg Each 20% Solution, Sterile 100 ml Each Maltose Monohydrate 100 g Each Maltose Monohydrate 500 g Each Maltose Monohydrate 1 kg Each TRYPSIN (0.25%) EDTA Trypsin (0.25%) EDTA (1X) is used to release adherent cells from tissue culture plasticware for harvesting and passaging. Contains 0.001% phenol red, 0.04% Trypsin, 0.25% EDTA. INORGANIC SALTS OTHER Trypsin (0.25%) EDTA (1X) VWRL Components g/l % of Solution NaCl 8 0.8% KCl % Na 2 HPO 4 7H 2 O % KH 2 PO 4 (Monobasic) % NaHCO % Phenol Red % EDTA 4Na 2H 2 O % Trypsin (1:250) % D-Glucose (Dextrose) 1 0.1% Trypsin (0.25%) EDTA (1X) 100 VWRL Each ALL YOU NEED Solutions for Science At VWR it is our mission to enable the advancement of science throughout the world. We do this by developing deep connections with our customers. We have a commitment to provide the best-in-class product portfolio, services, and operational efficiency to ensure that our customers can focus on their research. All You Need Solutions are a testament to this mission. We now offer a comprehensive range of products and services suited to a specific industry or application. Whether you are working in the Life Science, Analytical, or Production environments we have programs designed for you. Find out how VWR is helping the scientific community with All You Need Solutions! vwr.com/aynsolutions PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL VWR CELL CULTURE MEDIA AND REAGENTS 7

8 TISSUE CULTURE PLASTICWARE VWR Collection Cell Culture Volume and Area Guide Description VWR Standard Line Multiwell Cell Culture Plates Size Working Volume, ml Cell Growth Area, cm 2 Max Cell Yield** Cat. No. 6 Well 17.0 ml x Well 6.80 ml x Well 3.50 ml x Well 1.55 ml x Well, Flat Bottom 96 Well, U Bottom 0.39 ml x ml x Crystal clear virgin PS - sterile and certified non-pyrogenic Plasma treated for consistent cell attachment and growth Well volume uniformity ensures uniform growth surface area exposure Single position lid reduces misplacement and the risk of contamination VWR Standard Line Cell Culture Dishes VWR Standard Line Cell Culture Dishes, Gripping Ring for Improved Handling VWR Standard Line Cell Culture T-Flasks, Non-vented Caps 35 Dia. x 12 H, mm x Crystal clear virgin PS - sterile and 90 Dia. x 17 H, mm x certified non-pyrogenic Plasma treated for consistent cell 150 Dia. x 22 H, mm 60 Dia. x 18 H, mm 70 Dia. x 15 H, mm 100 Dia. x 22 H, mm x x x x attachment and growth Flat bottom and uniform wall thickness ensure dishes are distortion-free Optically clear, making them suitable for microscopy 12.5 cm 2 (25 ml) x Angled neck design offers good pipette 25 cm 2 (50 ml) x and cell scraper access 75 cm 2 (250 ml) x Plasma treated for consistent cell attachment and growth cm 2 (600 ml) x Crystal clear virgin PS - sterile and 300 cm 2 (850 ml) x certified non-pyrogenic Also available with vented caps VWR Roller Bottles, Surface Treated 2/Bag, 40/Case 1,000 ml x /Bag, 20/Case 2,000 ml x /Bag, 40/Case 2,000 ml x /Bag, 40/Case 2,000 ml x /Bag, 40/Case 2,000 ml (Ribbed Design) x Sterilized, non-pyrogenic Bottle surface is smooth and free from striation to maximize usable growth area Two different cap styles can be used in both open and closed systems Cat. No features a rib design VWR Erlenmeyer Flasks, Flat Base, Non-vented Caps VWR Erlenmeyer Flasks, Baffled Base*, Non-vented Caps * Baffled base flasks may allow higher working volumes or low shaking speeds ** Based on cell diameter 10µm 125 ml ml ml L L L ml ml ml L L L Virgin, medical-grade resin retains its clarity after being exposed to gamma radiation Non-pyrogenic, inert, and nonleaching Excellent for non-adhering/suspension cultures Also available with vented caps 2L and 3L flasks with convenient vented duo-cap makes switching caps easy; simply remove the dustguard cover to allow sterile exchange, then replace the cover M Lit. No