Anopheles freeborni. Courtesy
|
|
|
- Hubert West
- 6 years ago
- Views:
Transcription
1 Anopheles freeborni Courtesy
2 Plasmodia seen with the microscope M. Lontie, MCH, Leuven, 2012
3 Diagnosis of malaria Thin film (better for species identification). Thick film (more sensitive). QBC (quantitative buffy coat, BD). Antigen-detection. Molecular methods (New Microbiol :69.). Serology. (Platelet count: low in case of severe P. falciparum infection).
4 Thick film Blood (finger, tube), no anticoagulants. Immediately after sampling. Defibrination (at least during one minute). Not too thick. Allow drying. Quality of the water (ph). Also for Babesia, Borrelia, Trypanosoma, filaria (last pictures). U.S. Department of Health, Education, and Welfare.
5 Courtesy Peters W. & Gilles H.M.
6 Thick film Only with disposable fingerstick devices (Acta Clinica Belgica :63-69.). Can also be made with blood with anticoagulants (EDTA). Allow then much longer drying.
7 Thick film made with EDTA blood
8 Diagnosis of malaria 1 parasite / rbc = fever (nonimmune person) rbc in a thin film = 30 min. Thick film = concentration technique. Thick film = 10 to 30 times more sensitive than thin film. Thick film = 3 to 5 (10) min. U.S. Department of Health, Education, and Welfare.
9 Diagnosis of malaria 1 parasite / rbc = fever (non-immune person) = parasites / μl. Tolerance in immune persons = ( ) parasites / μl. Cerebral malaria in children (P. falciparum): parasites / μl associated with fatal issue. (Wéry, 1995).
10 Thick - thin film, objective 100 x wbc, rbc Giemsa 100 fields 0.25 μl / 10 min 2500 wbc rbc one = 4 parasites / μl 50 / μl = fever in nonimmune = 12.5 / film May-Grünwald-Giemsa 100 fields 0.01 μl / 10 min 100 wbc rbc one = 100 parasites / μl 50 / μl = fever in nonimmune = 0.5 / film Wéry M Protozoologie Médicale.
11 Diagnosis of malaria Geographical distribution of different species should be taken into account. Plasmodium falciparum limited to (sub-) tropical areas (summer isotherm of 20 C, altitude < 2000 m). Mixed infections are not uncommon (eg P. falciparum + P. ovale in West Africa).
12 Duffy blood group system = receptor P. vivax West & Central Africa Duffy bg: absent Plasmodium ovale East Africa Duffy bg: present Plasmodium vivax Wéry M Protozoologie Médicale.
13
14 MALARIA (falciparum) (vivax) falciparum vivax malariae (vivax) (malariae) vivax falciparum malariae vivax ovale falciparum malariae ovale According to M. Wéry, 1995
15 Malaria: antigens HRP-2: watersoluble histidine-rich protein from axesual stages and young gametocytes of P. falciparum (related to knob-associated HRP-1 and HRP-3). Pan-malarial antigen: pldh or aldolase, present in all four Plasmodium spp. A. Moody CMR, 15:66-78.
16 Malaria antigen (HRP2) detection Sensitivity: (and less) parasites / µl. False negatives due to very high parasitemias.
17
18 Diagnosis of malaria antigendetection Persistent positivity with gametocytes (JCM, 2001, 1025). PMA is expressed by P. malariae (JCM, 2001, 2035). Moody A CMR, 15: Rapid diagnostic tests for malaria parasites (review).
19 Lee N. et al JCM., 44: Effect of sequence variation in Plasmodium falciparum histidine-rich protein 2 on binding of specific monoclonal antibodies: implications for rapid diagnostic tests for malaria. Sequence variation can help to explain the variations in the performance of HRP-based RDTs and point toward possible solutions.
20 Prozone in 16 of 17 HRP-2 based RDTs P. Gillet Dissertation at the Maastricht University.
21 Malaria antigen detection Prozone: how to avoid? Always have a look at the thick film Perform always HRP2 & LDH or aldolase If low platelets look a second time at the thick film
22 Species-specific PCR diagnosis of malaria (CDC) 1. P. vivax 2. P. malariae 3. P. falciparum 4. P. ovale Two step nested PCR using the primers of Snounou et al. CDC, 2001.
23 Detection of four Plasmodium species in blood from humans by 18S rrna gene subunit-based and species-specific real time PCR assays Rougemont M. et al JCM, 42:
24 Malaria serology Only P. falciparum cultivated in vitro. To exclude malaria e.g. among travellers. Blood donation from travellers. For epidemiological reasons. Wéry M., 1995.
25 Malaria: staining procedures used Thick film: Giemsa staining. Thin film: May-Grünwald staining.
26 Inexpensive method of diluting Giemsa stain Petithory J.C. et al JCM, 43: bottles of Evian water. ph: 7.36 Ready to use and stable for weeks.
27 ph 7.2 = optimal ph : less coloured parasites and stippling ph 7.6: stronger coloured parasites and stippling
28 UK NEQAS Parasitology on the web Why is malariology so difficult? I firmly believe that knowledge and experience arises from constant practice and exposure to a wide variety of different forms of the parasites. The other problem is that no two strains of the same parasite are identical; intra-species variation is a characteristic of the malarial parasite.
29 Diagnosis of malaria Percentage of infected red blood cells. Ring stage. Trophozoite (growing ring stage). Schizont: number of merozoites: 2 (young) -24. Gametocytes: shape (crescentic ot rounded). Dots: James, Schüffner, Maurer. Pigment: P. malariae, P. ovale... Atypical elements. U.S. Department of Health, Education, and Welfare.
30 Parasitized RBC All: Plasmodium falciparum. Young (reticulocytes, larger rbc): Plasmodium ovale and Plasmodium vivax. Old (smaller rbc): Plasmodium malariae. Gentilini & Duflo
31 Plasmodium falciparum vivax ovale malariae RBC + > 40 % 2 % 2 % 1 % RBC Ring Trofozoite Schizont normal Maurer s spots 1, 2 / rbc with two nuclei not in peripheral blood not in peripheral blood enlarged Schüffner s dots enlarged Schüffner s dots, oval fimbriated smaller 1 / rbc 1 (2) / rbc 1 / rbc ameboid (2), merozoites ameboid pigment (2), 8-10 merozoites band shape pigment (2), 6-8 merozoites Gametocyte crescentic rounded rounded rounded
32 Courtesy Tulane
33 Sharing Plasmodium knowlesi? White N Sharing malarias. Lancet:1006. Singh B. et al Lancet: Monkey malaria in 120 humans in in Malaysian Borneo by PCR (by microscopy P. malariae).
34 Plasmodium knowlesi: the fifth human malaria parasite. N. J. White CID 46: Island of Borneo, Malaysia.
35 Plasmodium falciparum in thick film Courtesy U.S. Department of Health, Education and Welfare
36 Plasmodium falciparum: thick film with numerous small rings.
37 Plasmodium falciparum: thick film with numerous rings.
38 Plasmodium falciparum: ring in a thick film.
39 Plasmodium falciparum: ring and crescentic gametocyte in a thick film.
40 Plasmodium falciparum: two crescentic gametocytes in a thick film.
41 Plasmodium falciparum in thin film Courtesy U.S. Department of Health, Education and Welfare
42 Courtesy Lamy L.H.
43 Plasmodium falciparum: two rings and Maurer s spots in a thin film.
44 Plasmodium falciparum: four rings in a thin film.
45 Plasmodium falciparum: small young ring in a thin film.
46 Plasmodium falciparum: two «accolé» forms in one cell in a thin film.
47 Plasmodium falciparum: several rings, two in one red blood cell in a thin film.
48 Plasmodium falciparum: three rings in one red blood cell, ring with two nuclei, «accolé» form, two red blood cells with small rings in a thin film.
49 Plasmodium falciparum: numerous rings, three in one red blood cell in a thin film.
50 Plasmodium falciparum: numerous rings, four in one red blood cell in a thin film.
51 Plasmodium falciparum: numerous rings in a thin film.
52 Plasmodium falciparum: two small, and one «accolé-like» rings in a thin film.
53 Plasmodium falciparum: with very dark pigment collected in one small, dense block in a thin film.
54 Plasmodium falciparum: with very dark pigment collected in one small, dense block and in the process of initial chromatin division in a thin film.
55 Plasmodium falciparum: schizont in cord blood in a thin film.
56 Plasmodium falciparum: schizont with clumped pigment in peripheral blood. This is seen only in heavy infections (thin film).
57 Plasmodium falciparum: two rings and one gametocyte (thin film).
58 Plasmodium falciparum: one crescentic gametocyte, about 1.5 times diameter of erythrocyte in length (thin film).
59 Plasmodium falciparum: two crescentic gametocytes (thin film).
60 Plasmodium vivax in thin film Courtesy U.S. Department of Health, Education and Welfare
61 Courtesy Lamy L.H.
62 Plasmodium vivax: ring with Schüffner s stippling (thick film).
63 Plasmodium vivax: trophozoite with Schüffner s stippling (thick film).
64 Plasmodium vivax: young schizont, ameboid trophozoites and rings (thin film).
65 Plasmodium vivax: older ring and ameboid trophozoite (thin film).
66 Plasmodium vivax: older ring and ameboid trophozoite both with Schüffner s stippling (thin film).
67 Plasmodium vivax: three ameboid trophozoites in a thin film.
68 Plasmodium vivax: ameboid (vivax) trophozoite (thin film).
69 Plasmodium vivax: ameboid trophozoite, gametocyte and Schüffner s dots (thin film).
70 Plasmodium vivax: gametocyte (thin film).
71 Plasmodium vivax: three gametocytes (thin film).
72 Plasmodium vivax: schizont with many merozoites in an enlarged red blood cell (thin film).
73 Plasmodium vivax: schizont with many merozoites (thin film).
74 Plasmodium vivax: schizont with many merozoites (thin film).
75 Plasmodium vivax: mature schizont with many (19) merozoites (thin film).
76 Plasmodium vivax: disrupted schizont with many merozoites (thin film).
77 Plasmodium vivax: disrupted schizont (thin film).
78 Plasmodium ovale in thin film Courtesy U.S. Department of Health, Education and Welfare
79 Courtesy Lamy L.H.
80 Plasmodium ovale: ring with Schüffner s stippling (thick film).
81 Plasmodium ovale: young schizont with Schüffner s stippling (thick film).
82 Plasmodium ovale: ring (thin film).
83 Plasmodium ovale: ring in a rocket shaped cell (thin film).
84 Plasmodium ovale: enlarged oval red cell with one ring (thin film).
85 Plasmodium ovale: fimbriated red cell with one ring (thin film).
86 Plasmodium ovale: fimbriated oval red cell with one ring (thin film).
87 Plasmodium ovale: two rings in one cell (thin film).
88 Plasmodium ovale: fimbriated and enlarged oval red cell with one young trophozoite (thin film).
89 Plasmodium ovale: trophozoite (thin film).
90 Plasmodium ovale: trophozoite in a fimbriated red blood cell (thin film).
91 Plasmodium ovale: two ameboid trophozoites in fimbriated cells (thin film).
92 Plasmodium ovale: trophozoite in a fimbriated red blood cell (thin film).
93 Plasmodium ovale: gametocyte (thin film).
94 Plasmodium ovale: gametocyte (thin film).
95 Plasmodium ovale: one gametocyte and two trophozoites (thin film).
96 Plasmodium ovale: young schizont with two nuclei (thin film).
97 Plasmodium ovale: young schizont with three nuclei (thin film).
98 Plasmodium ovale: enlarged cell with schizont with seven merozoites and black pigment (thin film).
99 Plasmodium malariae in thin film Courtesy U.S. Department of Health, Education and Welfare
100 Courtesy Lamy L.H.
101 Plasmodium malariae: ring (thin film).
102 Plasmodium malariae: upper compact ring with black pigment and lower small ring (thin film).
103 Plasmodium malariae: young trophozoites with black pigment (thin film).
104 Plasmodium malariae: trophozoite with black pigment (thin film).
105 Plasmodium malariae: band shaped trophozoites (thin film).
106 Plasmodium malariae: band shaped trophozoite (thin film).
107 Plasmodium malariae: gametocyte (thin film).
108 Plasmodium malariae: gametocyte (thin film).
109 Plasmodium malariae: mature schizont in «marguerite form» with central black pigment (thin film).
110 Plasmodium malariae: mature schizont in «marguerite form» (thin film).
111 Malaria pigment (thin film). With heavy infections malaria pigment can be phagocytized by the leukocytes. Lawrence C. The Lancet, 1999, 353, 1852.
112 Plasmodium falciparum: in older blood exflagellation can sometimes be observed (thin film).
113 Babesia divergens (thin film). In the US ticks transmit Borrelia spp., Ehrlichia spp., and Babesia spp., and... Immunocompromised patients (asplenic). Generally low parasitemia. Microscopy (tetrad, Maltese cross), serology, molecular methods.
114 Babesia divergens (thin film). Over 100 Babesia spp. Babesia microti (NE and MW US). WA1 (Washington) related to canine B. gibsoni. Bovine Babesia divergens (Europe) and related MO1 (Missouri).
115 Babesia microti: the trophozoites are variable in size (thin film, PCR confirmed).
116 Babesia microti: multiply infected erythrocyte (thin film, PCR confirmed).
117 Babesia microti: extracellular trophozoites are common (thin film, PCR confirmed).
118 Borrelia duttoni (thin film).
119 Borrelia duttoni: are sometimes very slender (thick film).
120 Leishmania sp. Ovoid small (2-6 μm) parasites in a bone marrow aspirate. The typical rod shaped kinetoplast is seen beside the nucleus (May-Grünwald-Giemsa stain).
121 Trypanosoma brucei gambiense: kinetoplast, nucleus, and flagellum (thin film).
122 Trypanosoma cruzi: prominent kinetoplast, nucleus and flagellum (thin film).
123 Microfilariae that can be seen in peripheral blood in Congo-Kinshasa, according to J. Sonnet and J. Vandepitte. Mansonella perstans Loa loa Wuchereria bancrofti Periodicity day/night day night Vector flies (Culicoides spp.) flies (Chrysops spp.) mosquitoes (Culex, Anopheles, ) Size < 200 µm > 250 (230) µm > 250 µm Tail terminal nuclei terminal nuclei no terminal nuclei Sheath no yes (not stained by Giemsa) yes (slightly stained by Giemsa)
124 Mansonella perstans and Loa loa. Loa loa is distinctly longer and thicker than M. perstans. In the thick film the microfilaria of Loa loa show irregular coiling. The sheath does not stain with Giemsa (thick film stained with Giemsa).
125 2 Plasmodium? 3 Plasmodium? 4 Plasmodium? 5 Plasmodium? Courtesy Gentilini M. & Duflo B.
126 2 Plasmodium vivax 3 Plasmodium falciparum 4 Plasmodium malariae 5 Plasmodium ovale Courtesy Gentilini M. & Duflo B.
127 Comments mainly based on the Manual for the Microscopical Diagnosis of Malaria in Man 1960 Edition U.S. Department of Health, Education, and Welfare Public Health Service
Blood Smears Only 19 May Sample Preparation and Quality Control
NEW YORK STATE Parasitology Proficiency Testing Program Blood Smears Only 9 May 205 The purpose of the New York State Proficiency Testing Program in the category of Parasitology - Blood Smears Only is
Blood Smears Only 07 February Sample Preparation and Quality Control 12B A
NEW YORK STATE Parasitology Proficiency Testing Program Blood Smears Only 07 February 2012 The purpose of the New York State Proficiency Testing Program in the category of Parasitology Blood Smears Only
Blood Smears Only 5 February Sample Preparation and Quality Control 13B A
NEW YORK STATE Parasitology Proficiency Testing Program Blood Smears Only 5 February 2013 The purpose of the New York State Proficiency Testing Program in the category of Parasitology Blood Smears Only
Blood Smears Only 20 May Sample Preparation and Quality Control
NEW YORK STATE Parasitology Proficiency Testing Program Blood Smears Only 20 May 2014 The purpose of the New York State Proficiency Testing Program in the category of Parasitology - Blood Smears Only is
Blood Smears Only 3 February Sample Preparation and Quality Control
NEW YORK STATE Parasitology Proficiency Testing Program Blood Smears Only 3 February 2015 The purpose of the New York State Proficiency Testing Program in the category of Parasitology - Blood Smears Only
Blood Smears Only 6 October Sample Preparation and Quality Control 15B-K
NEW YORK STATE Parasitology Proficiency Testing Program Blood Smears Only 6 October 5 The purpose of the New York State Proficiency Testing Program in the category of Parasitology - Blood Smears Only is
Bloodborne Parasites: A New Perspective on Some Old Nemeses
Bloodborne Parasites: A New Perspective on Some Old Nemeses Ann Valley- Advanced Microbiologist Tim Monson- Microbiologist Supervisor Communicable Disease Division Wisconsin State Laboratory of Hygiene
CONTINUED FROM PART 1
CONTINUED FROM PART 1 1 LYNNE S. GARCIA, MS, CLS, FAAM Lynnegarcia2@verizon.net CAPHLD 65 th Institute Future Challenges in Diagnostic Medical Parasitology SPONSOR: MEDICAL CHEMICAL CORPORATION 2 MALARIA
BLOOD PARASITES. Danka Petrovic
BLOOD PARASITES Danka Petrovic Parasites Found in Blood Plasmodium species (Malaria) Babesia Trypanosoma spp Microfilariae Leishmania (Visceral) Plasmodium (Malaria) Four species are considered true parasites
ESCMID Online Lecture Library. by author
Laboratory diagnosis of Malaria handout 1 Lisette van Lieshout & Eric Brienen LvanLieshout@Lumc.nl healthy blood Microscopy thick blood smear haemolysis RBC + staining healthy blood Microscopy thin blood
No relevant conflicts of interest to disclose WISCONSIN STATE LABORATORY OF HYGIENE - UNIVERSITY OF WISCONSIN
No relevant conflicts of interest to disclose Disease background Diagnostics Contents Collecting blood Preparing smears Staining Interpreting slides Molecular and Antigen testing New and emerging causes
PARASITOLOGY CASE HISTORY #14 (BLOOD PARASITES) (Lynne S. Garcia)
PARASITOLOGY CASE HISTORY #14 (BLOOD PARASITES) (Lynne S. Garcia) A 37-year-old woman, who had traveled to New Guinea for several weeks, presented to the medical clinic with fever, chills, and rigors within
Protozoa from tissues. Leishmania spp. Naegleria fowleri Toxoplasma gondii Trichomonas vaginalis Trypanosoma spp.
Protozoa from tissues Leishmania spp. Naegleria fowleri Toxoplasma gondii Trichomonas vaginalis Trypanosoma spp. Leishmaniasis Leishmania infantum, Leishmania donovani, in macrophages of man. Female sandflies:
EDUCATIONAL COMMENTARY DISTINGUISHING MORPHOLOGIC LOOK-ALIKES
EDUCATIONAL COMMENTARY DISTINGUISHING MORPHOLOGIC LOOK-ALIKES Educational commentary is provided through our affiliation with the American Society for Clinical Pathology (ASCP). To obtain FREE CME/CMLE
Malaria Rapid Diagnostic Tests: role and place in the diagnosis of malaria
Malaria Rapid Diagnostic Tests: role and place in the diagnosis of malaria 1 Plasmodium falciparum Malaria : an overview 2 Plasmodium vivax 3 Plasmodium ovale Most serious Jan Jacobs Institute of Tropical
PARASITOLOGY CASE HISTORY #11 (BLOOD PARASITES) (Lynne S. Garcia)
PARASITOLOGY CASE HISTORY #11 (BLOOD PARASITES) (Lynne S. Garcia) A 39-year old male traveler developed fever and thrombocytopenia after returning from a trip to the Philippines. The parasitemia was 10,000
DISTANCE LEARNING ANSWER SHEET Please circle the one best answer for each question.
1 DISTANCE LEARNING ANSWER SHEET Please circle the one best answer for each question. COURSE NAME: POTENTIAL PROBLEMS WITH THE DIAGNOSIS OF MALARIA IN THE UNITED STATES COURSE # DL-002 NAME LIC. # DATE
Diagnosis of Infectious Agents in the Peripheral Blood Smear. Meredith Reyes, MD July 24, 2006
Diagnosis of Infectious Agents in the Peripheral Blood Smear Meredith Reyes, MD July 24, 2006 Pathogens detected in PS Bacteria Neisseria meningitidis Streptococcus pneumoniae Staphylococcus sp. Bartonella
HUMASIS MALARIA ANTIGEN TEST HIGH SENSITIVE DIFFERENTIAL DIAGNOSIS OF MALARIA INFECTION
HUMASIS MALARIA ANTIGEN TEST HIGH SENSITIVE DIFFERENTIAL DIAGNOSIS OF MALARIA INFECTION References 1) World Malaria Report 2010, WHO 2) Rapid diagnostic tests for malaria parasites, Clin. Microbiol. Rev.
Malaria Journal. Open Access. Abstract. BioMed Central
Malaria Journal BioMed Central Research Morphological features and differential counts of Plasmodium knowlesi parasites in naturally acquired human infections Kim-Sung Lee, Janet Cox-Singh and Balbir Singh*
Malaria rapid diagnostic test and Giemsa stained peripheral blood smear discrepancies in the diagnosis of Plasmodium ovale infection in New England
Malaria rapid diagnostic test and Giemsa stained peripheral blood smear discrepancies in the diagnosis of Plasmodium ovale infection in New England SEBLE AREGAWI, LEI LI, CATERINA M. MIRAGLIA ABSTRACT
Int.J.Curr.Microbiol.App.Sci (2015) 4(3):
ISSN: 2319-7706 Volume 4 Number 3 (2015) pp. 98-107 http://www.ijcmas.com Original Research Article Role of a Parasite Lactate Dehydrogenase-Based Immunochromatographic Antigen Detection Assay for the
A rapid test for the qualitative detection of Malaria pf and pv antigen in human blood sample
A rapid test for the qualitative detection of Malaria pf and pv antigen in human blood sample For in vitro use only Intended Use For the rapid qualitative determination of Malaria P. falciparum specific
Malaria (Pan-LDH) W/B
CORTEZ DIAGNOSTICS, INC. 23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302 USA Tel: (818) 591-3030 Fax: (818) 591-8383 E-mail: onestep@rapidtest.com Web site: www.rapidtest.com See external label
The Malarias: Plasmodium falciparum Plasmodium vivax Plasmodium malariae Plasmodium ovale. Distribution of Plasmodium falciparum
The Malarias: Plasmodium falciparum Plasmodium vivax Plasmodium malariae Plasmodium ovale Distribution of Plasmodium falciparum 1 Distribution Of Plasmodium vivax 2 Global Risk By Country-Proportionality
MalariaCare diagnostics refresher training learner s manual
The MalariaCare Toolkit Tools for maintaining high-quality malaria case management services MalariaCare diagnostics refresher training learner s manual Download all the MalariaCare tools from: www.malariacare.org/resources/toolkit.
Sysmex Educational Enhancement and Development No
SEED Malaria Sysmex Educational Enhancement and Development No 1 2017 Malaria diagnostics in the era of improved malaria control The purpose of this newsletter is to provide an overview of the role and
African Trypanosomes
African Trypanosomes Giemsa-stained blood smear of African trypanosomes viewed under the 100X objective lens. The block arrows denote trypomastigote forms of the African trypanosomes found within the blood
HEMOPOIETIC SYSTEM INFECTIONS BACTERIAL INFECTIONS OF THE BLOODSTREAM Reading Assignment: Chapters 50 & 63
HEMOPOIETIC SYSTEM INFECTIONS BACTERIAL INFECTIONS OF THE BLOODSTREAM Reading Assignment: Chapters 50 & 63 Definitions I. Bacteremia: Viable bacteria in the blood as demonstrated by a positive blood culture
NEW YORK STATE Parasitology Proficiency Testing Program. Parasitology (General) 01 February Sample Preparation and Quality Control
NEW YORK STATE Parasitology Proficiency Testing Program Parasitology (General) 01 February 2011 The purpose of the New York State Proficiency Testing Program in the category of Parasitology (General) is
Malaria parasites Malaria parasites are micro-organisms that belong to the genus Plasmodium. There are more than 100 species of Plasmodium, which can infect many animal species such as reptiles, birds,
The Malarias: Plasmodium falciparum Plasmodium vivax Plasmodium malariae Plasmodium ovale. Watersheds of the African Continent
The Malarias: Plasmodium falciparum Plasmodium vivax Plasmodium malariae Plasmodium ovale Global Risk By Country-Proportionality Plot P. falciparum P. vivax Distribution of Plasmodium falciparum 3 million
PARASITOLOGY CASE HISTORY #13 (BLOOD PARASITES) (Lynne S. Garcia)
PARASITOLOGY CASE HISTORY #13 (BLOOD PARASITES) (Lynne S. Garcia) An epidemiologic survey was undertaken in a small town in Myanmar (Burma) endemic for lymphatic filariasis. Blood specimens were collected
Laboratory diagnosis of Blood and tissue flagellates
Laboratory diagnosis of Blood and tissue flagellates (Leishmania and trypanosma) Sarah Alharbi Clinical Laboratory department Collage of Applied Medical Sciences King Saud University Leishmania and trypanosma:
Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK HSL (Analytics) LLP Haematology and Blood Transfusion Department Barnet Hospital Wellhouse Lane Barnet EN5 3DJ Contact: Priti Patel Tel: +44
Parasitic Protozoa, Helminths, and Arthropod Vectors
PowerPoint Lecture Slides for MICROBIOLOGY ROBERT W. BAUMAN Chapter 23 Parasitic Protozoa, Helminths, and Arthropod Vectors Parasitic Diseases Protozoan and helminthic parasites are emerging as serious
News and Notes. Parasitology Comprehensive 2 October Sample Preparation and Quality Control. 12 K (All Parasites)
NEW YORK STATE Parasitology Proficiency Testing Program News and Notes Recent reports in the literature have indicated a high rate of Cryptosporidium sp. false positive associated with Rapid Cartridge
In vitro cultivation of Plasmodium falciparum
Chapter 2 In vitro cultivation of Plasmodium falciparum In vitro cultivation of Plasmodium falciparum 2.1 INTRODUCTION Malaria represents the world s greatest public health problem in terms of number of
Comparison of light microscopy and nested PCR assay in detecting of malaria mixed species infections in an endemic area of Iran
Comparison of light microscopy and nested PCR assay in detecting of malaria mixed species infections in an endemic area of Iran Aliehsan Heidari, Manizheh Nourian, Hossein Keshavarz Associate Prof. Dept.
by author ESCMID Online Lecture Library Malaria Epidemiology, diagnosis and risk of resurgence Eskild Petersen Professor, MD, DMSc
Malaria Epidemiology, diagnosis and risk of resurgence Eskild Petersen Professor, MD, DMSc Department of Infectious Diseases Aarhus University Hospital, Skejby Specialist of Infectious Diseases and Tropical
AS NON MICROSCOPIC IMMUNOLOGICAL MARKER IN DIAGNOSIS OF MALARIAL PARASITE
International Journal of Medical Science and ducation An official Publication of Association for Scientific and Medical ducation (ASM) Original research Article ROL OF pldh ANTIGN AS NON MICROSCOPIC IMMUNOLOGICAL
Invest in the future, defeat malaria
Invest in the future, defeat malaria Malaria is caused by parasites from the genus Plasmodium, which are spread to people by infected mosquitoes. There are five species of Plasmodium that can infect humans.
Lesson 1:Introduction To Malaria INTRODUCTION. Contents. Objectives. From WikiEducator
Lesson 1:Introduction To Malaria From WikiEducator Contents 1 INTRODUCTION 2 Definition 2.1 The Malaria Life Cycle 2.2 Clinical Classification of Malaria 2.2.1 Conclusion: 2.2.2 Glossary 2.2.3 References
CLASSFICATION OF MALARIAL PARASITE AND ITS LIFE-CYCLE- STAGES IN BLOOD SMEAR
CLASSFICATION OF MALARIAL PARASITE AND ITS LIFE-CYCLE- STAGES IN BLOOD SMEAR 1 SRI HARTATI, 2 AGUS HARJOKO, 3 RIKA ROSNELLY, 4 IKA CANDRADEWI 1,2,3 Department of Computer Science and Electronics, Universitas
Usefulness of Modified Centrifuged Blood Smear in Diagnosis of Malaria
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 5 Number 3(2016) pp. 764-769 Journal homepage: http://www.ijcmas.com Original Research Article http://dx.doi.org/10.20546/ijcmas.2016.503.088
Malaria Case Studies
Malaria Case Studies Andrea K. Boggild, MSc, MD, DTMH, FRCPC Clinical Director, Tropical Disease Unit, UHN-TGH Assistant Professor, Department of Medicine, University of Toronto Parasitology Lead, Public
NEW YORK STATE Parasitology Proficiency Testing Program. Parasitology (General) 02 February Sample Preparation and Quality Control
NEW YORK STATE Parasitology Proficiency Testing Program Parasitology (General) 02 February 2010 The purpose of the New York State Proficiency Testing Program in the category of Parasitology (General) is
Malaria. An Overview of Life-cycle, Morphology and Clinical Picture
Malaria An Overview of Life-cycle, Morphology and Clinical Picture Malaria Malaria is the most important of all tropical parasitic disease,causes death and debility and is endemic throughout the tropics
Malaria. Population at Risk. Infectious Disease epidemiology BMTRY 713 (Lecture 23) Epidemiology of Malaria. April 6, Selassie AW (DPHS) 1
Infectious Disease Epidemiology BMTRY 713 (A. Selassie, DrPH) Lecture 23 Vector-Borne Disease (Part II) Epidemiology of Malaria Learning Objectives 1. Overview of malaria Global perspectives 2. Identify
MALARIA CONTROL FROM THE INDIVIDUAL TO THE COMMUNITY
MALARIA CONTROL FROM THE INDIVIDUAL TO THE COMMUNITY Calvin L. Wilson MD Clinical Professor of Family Medicine and Public Health University of Colorado Anschutz OBJECTIVES 1. Understand the unique characteristics
Subject BLOOD FILMS FOR MALARIA PREPARATION (INCLUDES BABESIA, EHRLICHIA (ANAPLASMA), TRYPANOSOMES, MICROFILARIAE)
Policy S:PC-2111 Fairview Health Services Department Laboratory /Nursing Section Acute Care: Collection Category Provision of Care Subject BLOOD FILMS FOR MALARIA PREPARATION (INCLUDES BABESIA, EHRLICHIA
Performance of Rapid Diagnostic Tests for Plasmodium ovale Malaria in Japanese Travellers
Tropical Medicine and Health Vol. 42 No.4, 2014, 149 153 doi: 10.2149/tmh.2014-07 Copyright 2014 by The Japanese Society of Tropical Medicine 149 Original Paper Performance of Rapid Diagnostic Tests for
Fabio T M Costa, PhD. University of Campinas (UNICAMP); Campinas, SP, Brazil. Supported by:
Fabio T M Costa, PhD University of Campinas (UNICAMP); Campinas, SP, Brazil Supported by: P. falciparum associated pathologies In falciparum malaria cytoadhesion of mature forms are related to fatalities!
Estimating the parasitaemia of Plasmodium falciparum: experience from a national EQA scheme
Manser et al. Malaria Journal 2013, 12:428 RESEARCH Open Access Estimating the of Plasmodium falciparum: experience from a national EQA scheme Monika Manser 1, Catherine Olufsen 1, Nick Andrews 2 and Peter
Impact of laboratory diagnosis for improving the management of uncomplicated malaria at peripheral health care settings in Coast region, Tanzania
From Department of Medicine, Solna Infectious Diseases Unit Karolinska University Hospital Karolinska Institutet, Stockholm, Sweden Impact of laboratory diagnosis for improving the management of uncomplicated
MALARIA PARASITE COUNTING
VERSION 1 EFFECTIVE DATE: 01/01/2016 MALARIA PARASITE COUNTING MALARIA MICROSCOPY STANDARD OPERATING PROCEDURE MM-SOP-09 1. PURPOSE AND SCOPE To describe the procedure for counting malaria parasites on
Malaria Pf/pan antigen Rapid Test
Malaria Pf/pan antigen Rapid Test Cat. No.:IVDTS003 Pkg.Size:10T/50T Intended use The Malaria Pf/pan antigen Rapid Test is a self-performing, qualitative, sandwich immunoassay, utilizing whole blood for
Babesia from a donor perspective
Babesia from a donor perspective American Red Cross, Massachusetts Region Bryan Spencer, MPH Research Scientist American Society for Apheresis Annual Meeting May 8, 2015 San Antonio, TX The need is constant.
Interventions for Babesia (and Plasmodium)
Interventions for Babesia (and Plasmodium) Susan L. Stramer, Ph.D. May 16 2017 www.aabb.org Babesiosis Malaria-like illness caused by Babesia spp. Asymptomatic fatal Non-specific symptoms (malaise, fever,
Trypanosomiasis WRAIR- GEIS 'Operational Clinical Infectious Disease' Course
Trypanosomiasis WRAIR- GEIS 'Operational Clinical Infectious Disease' Course UNCLASSIFIED Disclaimer The views expressed in this presentation are those of the speaker and do not reflect the official policy
Role of the Parasight-F Test in the Diagnosis of Complicated Plasmodium falciparum Malarial Infection
332 BJID 2003; 7 (October) Role of the Parasight-F Test in the Diagnosis of Complicated Plasmodium falciparum Malarial Infection Sandeep Arora 1, Manorama Gaiha 1 and Anju Arora 2 Maulana Azad Medical
Malaria Updates. Fe Esperanza Espino Department of Parasitology Research Institute for Tropical Medicine
Malaria Updates Fe Esperanza Espino Department of Parasitology Research Institute for Tropical Medicine Outline General epidemiology of malaria in the Philippines P falciparum Updates in treatment Recognizing
Fever, Headache, and Abdominal Pain in an African Male
Fever, Headache, and Abdominal Pain in an African Male Michael L. Cimo, MD, Rita Gander, PhD, Paul M. Southern, MD (Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX)
News and Notes. Parasitology Comprehensive 5 February Sample Preparation and Quality Control. 13 A Helminthes Only
NEW YORK STATE Parasitology Proficiency Testing Program News and Notes Recent reports in the literature have indicated a high rate of Cryptosporidium sp. false positive associated with Rapid Cartridge
Comparative Evaluation of Two Flowcytometric Analysers as Diagnostic Tools for the Automated Detection of Malaria
82 Available online at www.annclinlabsci.org Comparative Evaluation of Two Flowcytometric Analysers as Diagnostic Tools for the Automated Detection of Malaria S. Mohapatra 1, J. C. Samantaray 1, S. Arulselvi
Plasmodium knowlesi malaria in a traveller returning from the Philippines to Italy, 2016
Case Report Plasmodium knowlesi malaria in a traveller returning from the Philippines to Italy, 2016 Ettore De Canale 1, Dino Sgarabotto 2, Giulia Marini 2, Nicola Menegotto 3, Serena Masiero 3, Wassim
Malaria. Edwin J. Asturias, MD
Malaria Edwin J. Asturias, MD Associate Professor of Pediatrics and Epidemiology Director for Latin America Center for Global Health, Colorado School of Public Health Global Health and Disasters Course
MALARIA P. FALCIPARUM / P. VIVAX
MALARIA P. FALCIPARUM / P. VIVAX 1. EXPLANATION OF THE TEST: Malaria is a serious, sometimes fatal, parasitic disease characterized by fever, chills, and anemia and is caused by a parasite that is transmitted
Research Article Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR
Journal of Parasitology Research Volume 11, Article ID 104284, 6 pages doi:10.1155/11/104284 Research Article Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR
Malaria Combo Test Kit
CLIAwaived,Inc. Combo Test Kit A rapid test for the qualitative detection of Plasmodium falciparum and/or Plasmodium vivax, Plasmodium orale and Plasmodium malariae antigen in whole blood. For in-vitro
CLINICAL LAB INVESTIGATIONS: CASE STUDIES FOR THE LABORATORY PROFESSIONAL
CLINICAL LAB INVESTIGATIONS: CASE STUDIES FOR THE LABORATORY PROFESSIONAL CASE SET #25 A Parasitology Case: Now There Are Five This set of case studies is approved for 1.0 contact hour of P.A.C.E. credit.
The Morphologic Identification of Common Organisms That May Look Alike in the General Pathology Practice: A Brief Review
Ibnosina J Med BS 227 PATHOLOGY CORNER The Morphologic Identification of Common Organisms That May Look Alike in the General Pathology Practice: A Brief Review Jenna Boué 1, Basil M. Kahwash 2, Sean Kirby
Laboratory Diagnosis of Infections Due to Blood and Tissue Parasites
INVITED ARTICLE MEDICAL MICROBIOLOGY L. Barth Reller and Melvin P. Weinstein, Section Editors Laboratory Diagnosis of Infections Due to Blood and Tissue Parasites Jon E. Rosenblatt Division of Clinical
Fluorescence Based Methods for Rapid Diagnosis of Malaria
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 4 Number 8 (2015) pp. 832-839 http://www.ijcmas.com Original Research Article Fluorescence Based Methods for Rapid
Automatic Detection of Malaria Parasite from Blood Images
Volume 1, No. 3, May 2012 ISSN 2278-1080 The International Journal of Computer Science & Applications (TIJCSA) RESEARCH PAPER Available Online at http://www.journalofcomputerscience.com/ Automatic Detection
University of Veterinary and Animal Sciences, Bikaner), V.P.O. Bajor, Dist. Sikar, Rajasthan, India
REVIEW ARTICLE www.ijapc.com e-issn 2350-0204 Malaria, A Widely Prevalent Mosquito-Borne Infection in Humans and Recommended Herbal Therapy Subha Ganguly 1*, Satarupa Roy 2 1 Associate Department of Veterinary
Multiplex real-time PCR for the diagnosis of malaria: correlation with microscopy
ORIGINAL ARTICLE PARASITOLOGY Multiplex real-time PCR for the diagnosis of malaria: correlation with microscopy L. Dormond 1, K. Jaton-Ogay 1, S. de Vallière 2, B. Genton 2, J. Bille 1,3 and G. Greub 1,3
Malaria Life Cycle Life Cycle
Malaria Malaria Life Cycle Life Cycle Oocyst Zygote Sporogony Sporozoites Mosquito Salivary Gland Gametocytes Exoerythrocytic (hepatic) cycle Hypnozoites (for P. vivax and P. ovale) Erythrocytic Cycle
T he diagnosis of malaria in clinical laboratories in the UK
862 ORIGINAL ARTICLE Use of rapid diagnostic tests for diagnosis of malaria in the UK D Chilton, A N J Malik, M Armstrong, M Kettelhut, J Parker-Williams, P L Chiodini... See end of article for authors
WRAIR- GEIS 'Operational Clinical Infectious Disease' Course
Trypanosomiasis WRAIR- GEIS 'Operational Clinical Infectious Disease' Course The opinions or assertions contained herein are the private views of the author, and are not to be construed as official, or
FACTS. Approximately 2.48 million malaria cases are reported annually from South Asia. Of Which 75% cases are contributed by India alone.
MALARIA 2 FACTS Approximately 2.48 million malaria cases are reported annually from South Asia. Of Which 75% cases are contributed by India alone. The magnitude of the problem is further enhanced by P
Guide to the 1-3 Minute Blood Film Microscopic Review: Why and How?
Guide to the 1-3 Minute Blood Film Microscopic Review: Why and How? Dennis B. DeNicola, DVM, PhD, DACVP Chief Veterinary Educator IDEXX Laboratories, Inc. Westbrook, ME USA Adjunct Professor of Veterinary
TECHNICAL SERIES. Malaria and its diagnosis. ...Setting trends. Rapid tests for Malaria detection
For the use of Registered Medical Practioners and Laboratories only Malaria and its diagnosis TECHNICAL SERIES Rapid tests for Malaria detection Zephyr Biomedicals Gitanjali, Tulip Block, Dr. Antonio Do
Reducing Babesia and Malaria Risks: Testing, Donor Selection, Inactivation. Susan L. Stramer PhD IPFA 23 rd Meeting May
Reducing Babesia and Malaria Risks: Testing, Donor Selection, Inactivation Susan L. Stramer PhD IPFA 23 rd Meeting May 26 2016 Babesiosis Malaria-like illness caused by babesia spp. Asymptomatic fatal
Computer Vision for Malaria Parasite Classification in Erythrocytes
Computer Vision for Malaria Parasite Classification in Erythrocytes J.SOMASEKAR Department of Computer Science and Engineering (CSE) Gopalan College of Engineering and Management Whitefield, Bangalore,
L3-Malaria: Epidemiology, clinical management and control. 19 January 2018 SCBM346 TROPICAL INFECTIOUS DISEASES AND CONTROLS
19 January 2018 SCBM346 TROPICAL INFECTIOUS DISEASES AND CONTROLS L3-Malaria: Epidemiology, clinical management and control Lecturer: Niwat Kangwanrangsan, Ph.D. Department of Pathobiology Faculty of Science,
5/11/2009. Information About Malaria. What is Malaria? How many people think Malaria is caused by mosquitoes? Evolution & History
Information About Malaria Evolution & History Etiology, Host Specific Parasites & Symptoms & Pathology (Human) Treatments (Human) What is Malaria? How many people think Malaria is caused by mosquitoes?
اعداد رغداحمد رغد جمال الدين
اعداد رغداحمد رغد جمال الدين Trypanosoma Causes Trypanosomiasis West African Trypanosomiasis T.brucei gambiense Sleeping sickness East African Trypanosomiasis T.brucei rhodesiense American Trypanosomiasis
Parasitology. Helminthology (Helminths)
Parasitology Protozoology (Protozoa) Helminthology (Helminths) Entomology (Arthropodes) Platyhelminthes (flat worms) Nematheminthes (round worms) Trematodes Nematodes Cestodes Collection of the specimens
Protozoan Infections of the Circulatory System *
OpenStax-CNX module: m64867 1 Protozoan Infections of the Circulatory System * Geo Lin-Cereghino Based on Parasitic Infections of the Circulatory and Lymphatic Systems by OpenStax This work is produced
40% (90% (500 BC)
MALARIA causative agent = Plasmodium species 40% of world s population lives in endemic areas 3-500 million clinical cases per year 1.5-2.7 million deaths (90% Africa) known since antiquity early medical
ASSESSMENT OF AVIAN ANAEMIA
Vet Times The website for the veterinary profession https://www.vettimes.co.uk ASSESSMENT OF AVIAN ANAEMIA Author : KATE ENGLISH Categories : Vets Date : July 21, 2008 KATE ENGLISH discusses how anaemia
Correspondence should be addressed to Debjani Dasgupta;
Journal of Parasitology Research, Article ID 194651, 7 pages http://dx.doi.org/10.1155/2014/194651 Research Article Comparative Study of Modified Quantitative Buffy Coat and Two Rapid Tests in Comparison
4/16/2013 FARIDA OESMAN DEPARTMENT OF CLINICAL PATHOLOGY FACULTY OF MEDICINE, UNIVERSITY OF INDONESIA TYPHOID FEVER DENGUE FEVER MALARIA LEPTOSPIROSIS
FARIDA OESMAN DEPARTMENT OF CLINICAL PATHOLOGY FACULTY OF MEDICINE, UNIVERSITY OF INDONESIA TYPHOID FEVER DENGUE FEVER MALARIA LEPTOSPIROSIS NOT SPECIFIC DEFINITIVE HOST RESPONSE HEMATOLOGY ACUTE PHASE
FARIDA OESMAN DEPARTMENT OF CLINICAL PATHOLOGY FACULTY OF MEDICINE, UNIVERSITY OF INDONESIA
FARIDA OESMAN DEPARTMENT OF CLINICAL PATHOLOGY FACULTY OF MEDICINE, UNIVERSITY OF INDONESIA TYPHOID FEVER DENGUE FEVER MALARIA LEPTOSPIROSIS NOT SPECIFIC DEFINITIVE HOST RESPONSE HEMATOLOGY ACUTE PHASE
Characteristics of evaluation panel used for Round 4 of WHO Malaria RDT Product Testing at U.S. CDC,
Programme for Research and Training in Tropical Diseases (TDR) Malaria ranch, Division of Parasitic Diseases Centers for Characteristics of evaluation panel used for Round 4 of WHO Malaria RDT Product
ESCMID Online Lecture Library. by author
4-8 June 2012 T. van Gool, MD, PhD A. Bart, PhD Academic Medical Center Amsterdam F. Derouin, MD, PhD Hôpital Saint Louis Paris T. Kortbeek, MD RIVM, Bilthoven How good is training in Clinical Parasitology?
Color Atlas of Clinical Laboratory Medicine. First Version
Color Atlas of Clinical Laboratory Medicine First Version Addis Ababa, February 2015 Page 1 of 44 Table Contents 1. Hematology... 5 1.1. Cell Maturation... 5 1.2 Red Blood Cells Morphology... 6 1.3 Abnormal
Babesia and Blood Safety
Babesia and Blood Safety American Red Cross, New England Region Bryan Spencer, MPH Manager of Blood Research / REDS-III Coordinator ASM 46 th Annual Region I Meeting 27 October, 2011 Randolph, MA The need
Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Cher t s e y Lan e, Stai ne s - u pon-thames, TW18 3H R, UK
Schedule of Accreditation 2 Pine Trees, Cher t s e y Lan e, Stai ne s - u pon-thames, TW18 3H R, UK Department of Haematology & Blood Transfusion Levels 3 & 5 Torbay Hospital Lowe s Bridge Torquay TQ2