ISOLATION AND CHARACTERIZATION OF HESPERIDIN FROM ORANGE PEEL

Save this PDF as:
Size: px
Start display at page:

Download "ISOLATION AND CHARACTERIZATION OF HESPERIDIN FROM ORANGE PEEL"

Transcription

1 Indo American Journal of Pharmaceutical Research, 2013 ISSN NO: Journal home page: INDO AMERICAN JOURNAL OF PHARMACEUTICAL RESEARCH ISOLATION AND CHARACTERIZATION OF HESPERIDIN FROM ORANGE PEEL Prabhakar Sharma* 1, Prakash Pandey 1, Ramchandra Gupta 1, Sunil Roshan 1, Ashish Garg 2, Ajay Shulka 3, Anil Pasi 4 1 Department of Pharmacognosy 2 Department of pharmaceutics 3 Department of Pharmaceutical Chemistry, Guru Ramdas Khalsa Institute of Science and Technology (Pharmacy) Jabalpur, M.P. 4 College of pharmacy, IPS Academy, Indore, M.P. ARTICLE INFO Article history Received 13/05/2013 Available online 30/05/2013 ABSTRACT India is sitting on a gold mine of well-recorded and traditionally well practiced knowledge of herbal medicine. General phytochemical screening of Citrus sinensis (Orange) belonging Rutaceae family revealed the presence of flavones glycoside. The aim of this study is to identify and characterize the bioactive principle compound (Hesperidin) from the peel of C. sinensis fruit. It has wide folk medicinal uses. The isolation and characterization of phytoconstituents was done from the alkaline (KOH) extract of peels. Keywords Citrus sinensis, Hesperidin, Orange Peel, Orange Corresponding author: Prabhakar Sharma, ID: Contact No.: Please cite this article in press as Sharma et.al. Isolation and Characterization of Hesperidin from Orange Peel. Indo American Journal of Pharm Research.2013:3(4). Copy right 2013 This is an Open Access article distributed under the terms of the Indo American journal of Pharmaceutical Research, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Page3892

2 INTRODUCTION Throughout the ages, plants have been used by humans as a source of food, cosmetics, medicine, clothing and even shelter. Plant products also play an important role in the health care systems of the remaining 20% of the population who mainly reside in developed countries [1]. The plants of the genus citrus (Rutaceae) are widely distributed in most tropical and subtropical countries. Hesperidin, a flavanone-type polyphenolic bioflavonoid, is found abundant in the peel and membraneous parts of orange peel and other citrus fruits. Hesperidin is an antioxidant that enhances the action of vitamin C (ascorbic acid) to lower cholesterol levels. Hesperidin is comprised of the flavanone hesperitin and the disaccharide rutinose and has been reported to have many biologically important properties, including anti-inflammatory, antimicrobial, anticarcinogenic, antioxidant and capillary strengthening effects it is also known to have pharmacological action as an antiinflammatory, antihistaminic, and antiviral agent [2-5] as well as flavoring agent. MATERIAL AND METHOD 1) Collection of Plant Material: - The fruits of Citrus sinensis (Orange) were purchased from local market of Jabalpur, M.P and they were peeled off and peels were dried under shade. 2) Isolation of Hesperidin: - Hesperidin was isolated by the method given in literature and reference book with little modification. 2.1) Conventional Method: - The dried orange peels (250gm) were macerated with 800ml of aq. alkaline solution (10% KOH) ph 8-9 for overnight (fig. 1), After complete maceration the mixture was filtered by large Buchner funnel and filtrate was evaporated to make it syrupy mass [6]. Fig. 1 Maceration of orange peel 2.2) Modern Method: ml petroleum ether (40 60 C) is filled in a 250 ml round bottom flask with magnetic stir bar. 250g dried and powdered orange peel are placed in the extraction sleeve of a Soxhlet extractor and covered with a little glass wool. A reflux condenser is put on the Soxhlet extraction unit, and then the reaction mixture is stirred and heated for 4 hours under strong reflux (fig. 2). The petroleum ether extract is discarded. In order to remove the adherent petroleum ether, the content of the extraction sleeve is laid out in an extensive crystallisation dish. Afterwards the substance is placed again in an extraction sleeve and, like before, Page3893

3 but with 800 ml methanol, extracted unless the solvent leaving the extraction sleeve is color less (1 to 2 hours) [7]. Fig. 1 Soxhlation of orange peel After complete Soxhlet extraction and maceration the filtrate was then acidifying (ph 3-4) with 6% acetic acid, Keep the concentrated residual liquid in refrigerator (4-6 C) over night when a solid crystalline substance appears (fig. 3 and 4). It was again filtered and the crude hesperidin was separated out on Buchner funnel as amorphous powder. The hesperidin was further characterized and identified according to various physicochemical parameters [8]. The compound was characterized according to various factors. The yield of hesperidin was analyzed by graphical representation (fig. 5) Fig. 3 Hesperidin by conventional method fig. 4 Hesperidin by modern method Page3894

4 Yield 2.35 gm 1.8 gm conventional method modern method Fig. 5 Graphical representation of yield of hesperidin RESULT AND DISCUSSION The compound was isolated from maceration and Soxhlation shows a positive ferric chloride and Shinoda test for flavonoids, indicating that the compound may be a flavonoid [9-10]. It is yellow brown in color, characteristic aromatic in odor, with melting point 257 C. There are two spots were observed in thin layer chromatography of crystal using n-butanol: Acetic Acid: Water (3:1:1) as mobile phase at 0.20 and 0.62 (fig. 6), physical and analytical characters are listed in table 1 and 2 respectively. The yield of hesperidin from two different methods is 1.80 gm and 2.35 gm respectively. Table 1:- Physical Characters Sr. No Parameter Observation 1 Color Yellowish brown 2 Odor Aromatic and characteristic 3 Melting point 257 C 4 Yield 1.80 gm and 2.35 gm Table 2:- Analytical Characters Sr. No. Analytical Test Observation Result 1 Ferric chloride test Wine red color Present 2 Shinoda test Bright pinkish violet Present color 3 Thin layer chromatography [n-butanol: Acetic Acid: Water (3:1:1)] Two spots observed Rf value 0.62, 0.20 Page3895

5 Fig. 6 TLC of Hesperidin Page3896

6 REFERENCES 1. Kumarasamy, Y. et al, Screening seeds of Scottish plants for antibacterial activity. Journal of Ethnopharmacol, 83: Zhou, W. et al, 2011, Determination of Hesperidin from Pericarpium Citri Reticulatae by Microwave Assisted Extraction- High Performance Liquid Chromatography, Herald of Medicine, 30 (5), Seo, C.S. et al, 2011, Simultaneous Determination of Liquiritin, Hesperidin, and Glycyrrhizin by HPLC- Photodiode Array Detection and the Anti-Inflammatory Effect of Pyungwi-san. Arch. Pharmacal Res., 34 (2), Kakadiya J. et al, 2010, Effect of Hesperidin on Renal complication in Diabetes an Experimentally Study in Rats, Journal of Current Pharmaceutical Research; 3(1): Garg A et al, 2001, Chemistry and pharmacology of the citrus bioflavonoid hesperidin. Phytother Res.; 15: Jarald, E. Edvin and Jarald S. E., Textbook of Pharmacognosy For Phytochemistry, 2009, CBS Publishers And Distributers, Aghel N. et al, 2008, hesperidin from citrus sinnesis cultivated in dejful, iran, Pakistan journal of biological science, 11(20), Geissman, A. (Ed.,) (1962) The Chemistry of Flavonoid Compounds pp. 72 (New York: The MacMillan Company). 10. Markham, K. R. (ED.,) (1982) Techniques of Flavonoid Identification (London: Academic Press) Submit your next manuscript to IAJPR and take advantage of: Access Online first Double blind peer review policy No space constraints Rapid publication International recognition Submit your manuscript at: Page3897

THIN LAYER CHROMATOGRAPHY

THIN LAYER CHROMATOGRAPHY THIN LAYER CHROMATOGRAPHY Thin layer chromatography is the best known technique of plant biochemistry. TLC is used for preliminary separation and determination of plant constituents. It is helpful for

More information

QUANTITATIVE ESTIMATION OF PHYTOSTEROL FROM TWO MEDICINALLY IMPORTANT PLANTS OF CUCURBITACEAE

QUANTITATIVE ESTIMATION OF PHYTOSTEROL FROM TWO MEDICINALLY IMPORTANT PLANTS OF CUCURBITACEAE Int. J. Engg. Res. & Sci. & Tech. 2014 Renu Sarin and Sangeeta Samria, 2014 Research Paper ISSN 2319-5991 www.ijerst.com Vol. 3, No. 2, May 2014 2014 IJERST. All Rights Reserved QUANTITATIVE ESTIMATION

More information

Comparative HPTLC Analysis of Leaves of Allium cepa L., Ficus carica L. and Ziziphus mauritiana L. with Standard Quercetin

Comparative HPTLC Analysis of Leaves of Allium cepa L., Ficus carica L. and Ziziphus mauritiana L. with Standard Quercetin Available online on www.ijppr.com International Journal of Pharmacognosy and Phytochemical Research 2017; 9(5); 739-743 DOI number: 10.25258/phyto.v9i2.8157 ISSN: 0975-4873 Research Article Comparative

More information

Optimization of extraction method and profiling of plant phenolic compounds through RP-HPLC

Optimization of extraction method and profiling of plant phenolic compounds through RP-HPLC Chapter III Optimization of extraction method and profiling of plant phenolic compounds through RP-HPLC 1. INTRODUCTION Phenolics compounds are naturally present antioxidants, found in a variety of plant

More information

WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES. World Journal of Pharmacy and Pharmaceutical Sciences SJIF Impact Factor 6.

WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES. World Journal of Pharmacy and Pharmaceutical Sciences SJIF Impact Factor 6. WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES Neeru et al. SJIF Impact Factor 6.041 Volume 5, Issue 6, 1884-1891 Research Article ISSN 2278 4357 DETERMINATION OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID

More information

3. PRELIMINARY PHYTOCHEMICAL SCREENING

3. PRELIMINARY PHYTOCHEMICAL SCREENING 93 3. PRELIMINARY PHYTOCHEMICAL SCREENING 3.1 INTRODUCTION All the drugs- Ayurvedic, Unani and Herbal extracts were subjected to preliminary phytochemical screening to test the presence of alkaloids, carbohydrates

More information

Phytochemical Screening of Unripe Green Fruit Peel of Musa acuminata

Phytochemical Screening of Unripe Green Fruit Peel of Musa acuminata Serials Publications IJCE : 7(1), 2014: 11-15 Phytochemical Screening of Unripe Green Fruit Peel of Musa acuminata N. Gunavathy 1, S. Padmavathy 2 and S. C. Murugavel 3 1 Department of Chemistry, Nirmala

More information

Phytochemical screening and evaluation of leaves of Citrus limon (L.)Osbeck.

Phytochemical screening and evaluation of leaves of Citrus limon (L.)Osbeck. Research Paper ISSN: 2321-1520 Phytochemical screening and evaluation of leaves of Citrus limon (L.)Osbeck. Palak Sapra*, Shirin Qureshi, Archana Mankad and Hitesh Solanki *Department of Botany, Gujarat

More information

Research Article GALLIC ACID AND FLAVONOID ACTIVITIES OF AMARANTHUS GANGETICUS

Research Article GALLIC ACID AND FLAVONOID ACTIVITIES OF AMARANTHUS GANGETICUS ISSN 2395-3411 Available online at www.ijpacr.com 238 Research Article GALLIC ACID AND FLAVONOID ACTIVITIES OF AMARANTHUS GANGETICUS G. Jyoti Jain 1* and S. Ramachandra Setty 2 1 Department of Pharmacology,

More information

3016 Oxidation of ricinoleic acid (from castor oil) with KMnO 4 to azelaic acid

3016 Oxidation of ricinoleic acid (from castor oil) with KMnO 4 to azelaic acid 6 Oxidation of ricinoleic acid (from castor oil) with KMnO 4 to azelaic acid CH -(CH ) OH (CH ) -COOH KMnO 4 /KOH HOOC-(CH ) -COOH C H 4 O (.) KMnO 4 KOH (.) (6.) C H 6 O 4 (.) Classification Reaction

More information

Study of Phytochemical Screening and Antimicrobial Activity of Citrus aurantifolia Seed Extracts

Study of Phytochemical Screening and Antimicrobial Activity of Citrus aurantifolia Seed Extracts American Journal of Analytical Chemistry, 2016, 7, 254-259 Published Online March 2016 in SciRes. http://www.scirp.org/journal/ajac http://dx.doi.org/10.4236/ajac.2016.73022 Study of Phytochemical Screening

More information

Phytochemical Screening of Tubers and Leaf extracts of Sagittaria sagittifolial.:newsa (Arrowhead)

Phytochemical Screening of Tubers and Leaf extracts of Sagittaria sagittifolial.:newsa (Arrowhead) International Journal of Scientific and Research Publications, Volume 7, Issue 9, September 2017 431 Phytochemical Screening of Tubers and Leaf extracts of Sagittaria sagittifolial.:newsa (Arrowhead) Anita

More information

APPLICATION OF TLC METHOD WITH VIDEO SCANNING IN ESTIMATION OF DAILY DIETARY INTAKE OF SPECIFIC FLAVONOIDS ñ PRELIMINARY STUDIES

APPLICATION OF TLC METHOD WITH VIDEO SCANNING IN ESTIMATION OF DAILY DIETARY INTAKE OF SPECIFIC FLAVONOIDS ñ PRELIMINARY STUDIES Acta Poloniae Pharmaceutica ñ Drug Research, Vol. 70 No. 4 pp. 611ñ620, 2013 ISSN 0001-6837 Polish Pharmaceutical Society ANALYSIS APPLICATION OF TLC METHOD WITH VIDEO SCANNING IN ESTIMATION OF DAILY DIETARY

More information

Development of Supercritical Fluid Extraction of Glabridin from Glycyrrhiza glabra

Development of Supercritical Fluid Extraction of Glabridin from Glycyrrhiza glabra Development of Supercritical Fluid Extraction of Glabridin from Glycyrrhiza glabra On-line Number 939 Yun-Kyoung Cho, 1 Sang-Yun Lee, 1,2 Hyun-Seok Kim, 3 Jong-Hoon Ryu, 1 and Gio-Bin Lim 1 1 Department

More information

Synthesis and Blastocyst Implantation Inhibition Potential of Lupeol Derivatives in Female Mice

Synthesis and Blastocyst Implantation Inhibition Potential of Lupeol Derivatives in Female Mice Supporting Information Rec. Nat. Prod. 9:4 (2015) 561-566 Synthesis and Blastocyst Implantation Inhibition Potential of Lupeol Derivatives in Female Mice Anita Mahapatra 1*, Purvi Shah 1, Mehul Jivrajani

More information

*Corres.author: Mobile-: ,

*Corres.author: Mobile-: , International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol.6, No.2, pp 881-885, April-June 2014 Production of Flavonoids from Terminalia arjuna (ROXB.) in vivo and in vitro Tissue

More information

Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:

Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: Banu Rekha J and Jaykar. / Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry. 3(2), 2015, 76-81. Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and

More information

ISSN Volume 2 (2015) The 3rd International Conference on Biological Science 2013 (The 3rd ICBS-2013)

ISSN Volume 2 (2015) The 3rd International Conference on Biological Science 2013 (The 3rd ICBS-2013) ISSN 2413-0877 Volume 2 (2015) 129-133 The 3rd International Conference on Biological Science 2013 (The 3rd ICBS-2013) DETECTION OF ALKALOID, FLAVONOID, AND TERPENOID COMPOUNDS IN BREAD (Artocarpus communis

More information

Mohan Manvitha. Int. Res. J. Pharm. 2013, 4 (12) INTERNATIONAL RESEARCH JOURNAL OF PHARMACY

Mohan Manvitha. Int. Res. J. Pharm. 2013, 4 (12) INTERNATIONAL RESEARCH JOURNAL OF PHARMACY INTERNATIONAL RESEARCH JOURNAL OF PHARMACY www.irjponline.com ISSN 2230 8407 Research Article DETERMINATION OF ANDROGRAPHOLIDE IN ANDROGRAPHIS PANICULATA EXTRACTS WITH AND WITHOUT HUMAN SERUM BY HIGH PERFORMANCE

More information

Standardisation of Herbal Extract Mixture by HPTLC Method

Standardisation of Herbal Extract Mixture by HPTLC Method Research Article Standardisation of Herbal Extract Mixture by HPTLC Method S. Dhanalakshmi*, Jayakumari S., Abinaya S.K, Srudhi, Elenchizeyan Dept of Pharmacognosy, School of Pharmaceutical Sciences, (VISTAS),

More information

SIMAROUBA CEDRON FOR HOMOEOPATHIC PREPARATIONS CEDRON FOR HOMOEOPATHIC PREPARATIONS

SIMAROUBA CEDRON FOR HOMOEOPATHIC PREPARATIONS CEDRON FOR HOMOEOPATHIC PREPARATIONS SIMAROUBA CEDRON FOR HOMOEOPATHIC PREPARATIONS CEDRON FOR HOMOEOPATHIC PREPARATIONS Simaba cedron ad praeparationes homoeopathicas Other Latin name used in homoeopathy: Simaruba DEFINITION Dried cotyledons

More information

IJPRD, 2011; Vol 4(03): May-2012 ( ) International Standard Serial Number

IJPRD, 2011; Vol 4(03): May-2012 ( ) International Standard Serial Number IJPRD, 2011; Vol 4(03): May-2012 (024-028) International Standard Serial Number 0974 9446 --------------------------------------------------------------------------------------------------------------------------------------------------

More information

CHEMICAL STANDARDIZATION AND QUANTIFICATION OF PIPERIN FROM METHANOLIC EXTRACT OF PIPER NIGRUM BY HPLC METHOD ON THE BASIS OF ISOLATED MARKERS

CHEMICAL STANDARDIZATION AND QUANTIFICATION OF PIPERIN FROM METHANOLIC EXTRACT OF PIPER NIGRUM BY HPLC METHOD ON THE BASIS OF ISOLATED MARKERS Int. J. Chem. Sci.: 6(4), 2008, 1726-1733 CHEMICAL STANDARDIZATION AND QUANTIFICATION OF PIPERIN FROM METHANOLIC EXTRACT OF PIPER NIGRUM BY HPLC METHOD ON THE BASIS OF ISOLATED MARKERS RAJANI CHAUHAN,

More information

12BL Experiment 2: Extraction & Saponification of Trimyristin from Nutmeg

12BL Experiment 2: Extraction & Saponification of Trimyristin from Nutmeg 12BL Experiment 2: Extraction & Saponification of Trimyristin from Nutmeg Safety: Proper lab goggles/glasses must be worn (even over prescription glasses). Heating of organic solvents releases irritating

More information

Amudha S et al., Asian Journal of Pharmthiaceutical Technology & Innovation, 04 (21); 2016; Research Article

Amudha S et al., Asian Journal of Pharmthiaceutical Technology & Innovation, 04 (21); 2016; Research Article Asian Journal of Pharmaceutical Technology & Innovation ISSN: 2347-8810 Research Article Received on: 09-11-2016 Accepted on: 20-11-2016 Published on: 15-12-2016 Corresponding Author: * Amudha S, Dept.

More information

In Vitro Antioxidant Activity and Phytochemical Screening of Pholidota articulata

In Vitro Antioxidant Activity and Phytochemical Screening of Pholidota articulata Human Journals Research Article November 2016 Vol.:5, Issue:1 All rights are reserved by Darshan Singh et al. In Vitro Antioxidant Activity and Phytochemical Screening of Pholidota articulata Keywords:

More information

Received June 8, 1957

Received June 8, 1957 SELECTIVE DEMETHYLATION OF THE 5-METHOXYL GROUP IN FLAVANONES AND SYNTHESIS OF DIHYDROWOGONIN BY S. N. AIYAR, ISHWAR DASS AND T. R. SESHADRI, F.A.Sc. (Department of Chemistry, University of Delhi, Delhi)

More information

In vitro antioxidant activity of a flavonoid compound isolated from methanolic extract of Helianthus annuus leaves (Asteraceae)

In vitro antioxidant activity of a flavonoid compound isolated from methanolic extract of Helianthus annuus leaves (Asteraceae) Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2017, 9 [6]:28-34 [http://scholarsresearchlibrary.com/archive.html] ISSN 0975-5071 USA CODEN: DPLEB4

More information

ARTESUNATE TABLETS: Final text for revision of The International Pharmacopoeia (December 2009) ARTESUNATI COMPRESSI ARTESUNATE TABLETS

ARTESUNATE TABLETS: Final text for revision of The International Pharmacopoeia (December 2009) ARTESUNATI COMPRESSI ARTESUNATE TABLETS December 2009 ARTESUNATE TABLETS: Final text for revision of The International Pharmacopoeia (December 2009) This monograph was adopted at the Forty-fourth WHO Expert Committee on Specifications for Pharmaceutical

More information

Unit I (b) Paper Chromatography

Unit I (b) Paper Chromatography Unit I (b) Paper Chromatography Presentation by Mr. VELLURU REDDY MOHAN Assistant Professor Department of Pharmaceutical Analysis Krishna Teja Pharmacy college Subject : PHARMACEUTICAL ANALYSIS- II (15R00602)

More information

Pharmacognostic and preliminary phytochemical analysis of Aegle marmelos L. and Centella asiatica L.

Pharmacognostic and preliminary phytochemical analysis of Aegle marmelos L. and Centella asiatica L. Pharmacognostic and preliminary phytochemical analysis of Aegle marmelos L. and Centella asiatica L. A. Vijayalakshmi, G. Sakthivigneswari and Suganya, J. Department of Botany, Avinashilingam Institute

More information

SOLVENT EXTRACTION OF MANGO (MANGIFERA INDICA L.) SEED KERNEL OIL AND ITS CHARACTERIZATION 1

SOLVENT EXTRACTION OF MANGO (MANGIFERA INDICA L.) SEED KERNEL OIL AND ITS CHARACTERIZATION 1 SOLVENT EXTRACTION OF MANGO (MANGIFERA INDICA L.) SEED KERNEL OIL AND ITS CHARACTERIZATION D.C. Sikdar, 2 Siri Hegde, 3 Vinay Swamynathan, 4 Varsha S, 5 Rao Rakesh Dept. of Chemical Engineering, Dayananda

More information

ANALYTICAL METHOD DEVELOPMENT FOR CHIRAL SEPARATIONS OF FRUITY FLAVONOIDS USING RP-HPLC WITH IMPROVED SELECTIVITY AND SENSITIVITY

ANALYTICAL METHOD DEVELOPMENT FOR CHIRAL SEPARATIONS OF FRUITY FLAVONOIDS USING RP-HPLC WITH IMPROVED SELECTIVITY AND SENSITIVITY ABSTRACT Research Article ANALYTICAL METHOD DEVELOPMENT FOR CHIRAL SEPARATIONS OF FRUITY FLAVONOIDS USING RP-HPLC WITH IMPROVED SELECTIVITY AND SENSITIVITY 1 Shrikant R. Kulkarni, 2 Anil W Kharche Address

More information

Synthesis and Analysis of N-Acetyltyrosine-N- Ethyl Amide from N-Acetyl Yyrosine Ethyl Ester

Synthesis and Analysis of N-Acetyltyrosine-N- Ethyl Amide from N-Acetyl Yyrosine Ethyl Ester Governors State University OPUS Open Portal to University Scholarship All Capstone Projects Student Capstone Projects Fall 2010 Synthesis and Analysis of N-Acetyltyrosine-N- Ethyl Amide from N-Acetyl Yyrosine

More information

Determination of Tanninoids. Analytical Pharmacognosy

Determination of Tanninoids. Analytical Pharmacognosy QUIZ If the manager of a phytopharmaceutical industry wish to buy one chromatographic equipment, which one you recommend, HPLC or TLC densitometer. What are the reasons to support your recommendation.

More information

9. Determine the mass of the fat you removed from the milk and record in the table. Calculation:

9. Determine the mass of the fat you removed from the milk and record in the table. Calculation: Chemistry 100 Instructor s Initials Name: Experiment 14: Biochemistry Analysis of milk for the lipids, carbohydrates, and proteins. A. Determining the % Fat in Whole Milk 1. Weigh a clean, dry, empty 50

More information

DETERMINATION OF MYCOTOXIN FROM FEED SAMPLE BY THIN LAYER CHROMATOGRAPHY

DETERMINATION OF MYCOTOXIN FROM FEED SAMPLE BY THIN LAYER CHROMATOGRAPHY Indo-Am. J. Agric. & Vet. Sci., 2014 Ashok Kumar ISSN D and 2321 9602 E Sunil anand www.iajavs.com Kumar, 2014 Vol. 2, No. 1, March 2014 2014 Meghana Publications. All Rights Reserved Research Paper DETERMINATION

More information

Supporting Information for. Boronic Acid Functionalized Aza-Bodipy (azabdpba) based Fluorescence Optodes for the. analysis of Glucose in Whole Blood

Supporting Information for. Boronic Acid Functionalized Aza-Bodipy (azabdpba) based Fluorescence Optodes for the. analysis of Glucose in Whole Blood Supporting Information for Boronic Acid Functionalized Aza-Bodipy (azabdpba) based Fluorescence Optodes for the analysis of Glucose in Whole Blood Yueling Liu, Jingwei Zhu, Yanmei Xu, Yu Qin*, Dechen Jiang*

More information

Novel D-erythro N-Octanoyl Sphingosine Analogs As Chemo- and Endocrine. Resistant Breast Cancer Therapeutics

Novel D-erythro N-Octanoyl Sphingosine Analogs As Chemo- and Endocrine. Resistant Breast Cancer Therapeutics Page 11 of 32 Cancer Chemotherapy and Pharmacology Novel D-erythro N-Octanoyl Sphingosine Analogs As Chemo- and Endocrine Resistant Breast Cancer Therapeutics James W. Antoon, Jiawang Liu, Adharsh P. Ponnapakkam,

More information

Journal of Pharmacognosy and Phytochemistry. Preliminary Phytochemical Screening and HPTLC Fingerprinting of Leaf Extracts of Pisonea aculeata

Journal of Pharmacognosy and Phytochemistry. Preliminary Phytochemical Screening and HPTLC Fingerprinting of Leaf Extracts of Pisonea aculeata ISSN 2278-4136 ZDB-Number: 2668735-5 IC Journal No: 8192 Volume 2 Issue 1 Online Available at www.phytojournal.com Journal of Pharmacognosy and Phytochemistry Preliminary Phytochemical Screening and HPTLC

More information

COMMON BARBERRY FOR HOMOEOPATHIC PREPARATIONS BERBERIS VULGARIS FOR HOMOEOPATHIC PREPARATIONS

COMMON BARBERRY FOR HOMOEOPATHIC PREPARATIONS BERBERIS VULGARIS FOR HOMOEOPATHIC PREPARATIONS COMMON BARBERRY FOR HOMOEOPATHIC PREPARATIONS BERBERIS VULGARIS FOR HOMOEOPATHIC PREPARATIONS Berberis vulgaris ad praeparationes homoeopathicas Other Latin name used in homoeopathy : Berberis DEFINITION

More information

Phenolic Pigment Extraction from Orange Peels: Kinetic Modeling

Phenolic Pigment Extraction from Orange Peels: Kinetic Modeling 15 th International Conference on Environmental Science and Technology Rhodes, Greece, 31 August to 2 September 2017 Phenolic Pigment Extraction from Orange Peels: Kinetic Modeling Kodal S. P. 1, Aksu

More information

Asian Journal of Pharmaceutical Science & Technology

Asian Journal of Pharmaceutical Science & Technology Asian Journal of Pharmaceutical Science & Technology e-issn: 2248 9185 www.ajpst.com Print ISSN: 2248 9177 TLC AND HPLC FINGERPRINT DEVELOPMENT OF TRACHYSPERMUM AMMI LINN. AND ITS POLYHERBAL MARKETED FORMULATIONS,

More information

Journal of Global Trends in Pharmaceutical Sciences

Journal of Global Trends in Pharmaceutical Sciences An Elsevier Indexed Journal ISSN-2230-7346 Journal of Global Trends in Pharmaceutical Sciences Research Article STIGMASTEROL, CAMPESTEROL AND BRASSICOSTEROL FROM HIBISCUS SABDARIFFA SEED Devarakonda Ramadevi*

More information

Application Note. Treatment of poor memory, memory loss, Alzheimer s disease, peripheral vascular disease.

Application Note. Treatment of poor memory, memory loss, Alzheimer s disease, peripheral vascular disease. Application Note As published in The Handbook of Analytical Methods for Dietary Supplements 0047 - Ginkgo for Flavonoids and Terpenes by HPLC Botanical Name: Ginkgo biloba L. Common Names: Maidenhair tree,

More information

Available online Research Article

Available online   Research Article Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2015, 7(12):519-523 Research Article ISSN : 0975-7384 CDEN(USA) : JCPRC5 Characterization of cyanidin 3-(6-acetylglucoside)-5-(3

More information

Petrolatum. Stage 4, Revision 1. Petrolatum is a purified semi solid mixture of hydrocarbons obtained from petroleum.

Petrolatum. Stage 4, Revision 1. Petrolatum is a purified semi solid mixture of hydrocarbons obtained from petroleum. 1 001-1208PDG.pdf Petrolatum Stage 4, Revision 1 Definition Petrolatum is a purified semi solid mixture of hydrocarbons obtained from petroleum. It may contain a suitable antioxidant. Description and Solubility

More information

Good pharmacopoeial practices: Chapter on monographs on herbal medicines

Good pharmacopoeial practices: Chapter on monographs on herbal medicines Annex 7 Good pharmacopoeial practices: Chapter on monographs on herbal medicines Background Following the fiftieth meeting of the WHO Expert Committee on Specifications for Pharmaceutical Preparations,

More information

Asian Journal of Food and Agro-Industry ISSN Available online at

Asian Journal of Food and Agro-Industry ISSN Available online at As. J. Food Ag-Ind. 2009, 2(03), 336-341 Asian Journal of Food and Agro-Industry ISSN 1906-3040 Available online at www.ajofai.info Short Communication In-vitro cytotoxicity (LC 50 ) of extracts obtained

More information

SUPPLEMENTARY MATERIAL Antiradical and antioxidant activity of flavones from Scutellariae baicalensis radix

SUPPLEMENTARY MATERIAL Antiradical and antioxidant activity of flavones from Scutellariae baicalensis radix SUPPLEMENTARY MATERIAL Antiradical and antioxidant activity of flavones from Scutellariae baicalensis radix Dorota Woźniak A, Andrzej Dryś B, and Adam Matkowski* A A Department of Pharmaceutical Biology

More information

Preparation and characterization of Aloe vera extract

Preparation and characterization of Aloe vera extract Chapter 2 Preparation and characterization of Aloe vera extract 2.1. INTRODUCTION Aloe vera ethanolic extract was prepared according to Noor et.al, 2008 with few modifications. The phytochemical analysis

More information

THE ANALYSIS OF CAROTENOIDS FROM MINT EXTRACTS. Abstract

THE ANALYSIS OF CAROTENOIDS FROM MINT EXTRACTS. Abstract M. Ciurea, et al. Scientifical Researches. Agroalimentary Processes and Technologies, Volume XI, No. 2 (2005), 261-266 THE ANALYSIS OF CAROTENOIDS FROM MINT EXTRACTS Mihaela Ciurea 1, I. Jianu 2 1 Aurel

More information

CELLULOSE, MICROCRYSTALLINE. Cellulosum microcristallinum. Cellulose, microcrystalline EUROPEAN PHARMACOPOEIA 7.0

CELLULOSE, MICROCRYSTALLINE. Cellulosum microcristallinum. Cellulose, microcrystalline EUROPEAN PHARMACOPOEIA 7.0 Cellulose, microcrystalline EUROPEAN PHARMACOPOEIA 7.0 Phthaloyl groups (C 8 H 5 O 3 ; M r 149.1): typically 30.0 per cent to 36.0 per cent (anhydrous and acid-free substance). Dissolve 1.000 g in 50 ml

More information

NEW JERSEY TEA, DRIED FOR HOMOEOPATHIC PREPARATIONS CEANOTHUS AMERICANUS SICCUM FOR HOMOEOPATHIC PREPARATIONS

NEW JERSEY TEA, DRIED FOR HOMOEOPATHIC PREPARATIONS CEANOTHUS AMERICANUS SICCUM FOR HOMOEOPATHIC PREPARATIONS NEW JERSEY TEA, DRIED FOR HOMOEOPATHIC PREPARATIONS CEANOTHUS AMERICANUS SICCUM FOR HOMOEOPATHIC PREPARATIONS Ceanothus americanus siccum ad praeparationes homoeopathicas Other Latin name used in homoeopathy

More information

COCHINEAL FOR HOMOEOPATHIC PREPARATIONS COCCUS CACTI FOR HOMOEOPATHIC PREPARATIONS

COCHINEAL FOR HOMOEOPATHIC PREPARATIONS COCCUS CACTI FOR HOMOEOPATHIC PREPARATIONS COCHINEAL FOR HOMOEOPATHIC PREPARATIONS COCCUS CACTI FOR HOMOEOPATHIC PREPARATIONS Coccus cacti ad praeparationes homoeopathicas DEFINITION Whole, dried, female insect, Coccus cacti L. (Dactylopius coccus

More information

Available online at Phytochemical analysis and inhibitory activity of Ornamental Plant (Bougainvillea spectabilis)

Available online at   Phytochemical analysis and inhibitory activity of Ornamental Plant (Bougainvillea spectabilis) Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 13, 3(2):1- ISSN : 2249-7412 CODEN (USA): AJPSKY Phytochemical analysis and inhibitory activity of Ornamental

More information

Phytochemical Analysis and Antioxidant property of Aegle marmelos Extracts

Phytochemical Analysis and Antioxidant property of Aegle marmelos Extracts ISSN: 2319-7706 Volume 4 Number 9 (2015) pp. 826-830 http://www.ijcmas.com Original Research Article Phytochemical Analysis and Antioxidant property of Aegle marmelos Extracts Anjay Kumar Gupta*, Sumeet

More information

AMERICAN SPIKENARD FOR HOMOEOPATHIC PREPARATIONS ARALIA RACEMOSA FOR HOMOEOPATHIC PREPARATIONS

AMERICAN SPIKENARD FOR HOMOEOPATHIC PREPARATIONS ARALIA RACEMOSA FOR HOMOEOPATHIC PREPARATIONS AMERICAN SPIKENARD FOR HOMOEOPATHIC PREPARATIONS ARALIA RACEMOSA FOR HOMOEOPATHIC PREPARATIONS Aralia racemosa ad praeparationes homoeopathicas DEFINITION Dried, underground part of Aralia racemosa L.

More information

HPTLC and HPLC analysis of T. chebula extracts prepared using microwave and ultrasonication assisted extraction methods

HPTLC and HPLC analysis of T. chebula extracts prepared using microwave and ultrasonication assisted extraction methods 215; 4(1): 192-196 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 215; 4(1): 192-196 Received: 12-4-215 Accepted: 15-5-215 Rency Elizabeth Thomas Department of Biotechnology, S. D. Kamat Department of Microbiology,

More information

Isolation and Identification of Anthralin From the Roots of Rhubarb Plant (Rheum palmatum)

Isolation and Identification of Anthralin From the Roots of Rhubarb Plant (Rheum palmatum) ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net Vol. 4, No. 4, pp. 546-549, October 2007 Isolation and Identification of Anthralin From the Roots of Rhubarb Plant (Rheum palmatum)

More information

Screening of Antimicrobials of some Medicinal Plants by TLC Bioautography

Screening of Antimicrobials of some Medicinal Plants by TLC Bioautography Screening of Antimicrobials of some Medicinal Plants by TLC Bioautography Middha Himanshu 1* and Parihar Pradeep 2 1Department of Microbiology, DTM College of Biosciences, Bikaner, Rajasthan 2 Lovely Professional

More information

Rapid Gradient and Elevated Temperature UHPLC of Flavonoids in Citrus Fruit

Rapid Gradient and Elevated Temperature UHPLC of Flavonoids in Citrus Fruit Rapid Gradient and Elevated Temperature UHPLC of Flavonoids in Citrus Fruit Application Note General Chromatography, Food Industry Authors John W. Henderson Jr., Judy Berry, Anne Mack, William Long Agilent

More information

Title Revision n date

Title Revision n date A. THIN LAYER CHROMATOGRAPHIC TECHNIQUE (TLC) 1. SCOPE The method describes the identification of hydrocortisone acetate, dexamethasone, betamethasone, betamethasone 17-valerate and triamcinolone acetonide

More information

ACUTE ORAL TOXICITY STUDY OF AQUEOUS AND METHANOLIC EXTRACT OF BLUMEA VIRENS IN SPRAGUE DAWLEY RATS

ACUTE ORAL TOXICITY STUDY OF AQUEOUS AND METHANOLIC EXTRACT OF BLUMEA VIRENS IN SPRAGUE DAWLEY RATS Explor Anim Med Res, Exploratory Animal and Medical Research, Vol.7, Issue 2, December, 2017 Vol.7, Issue - 2, 2017, p. 154-158 Research Article ACUTE ORAL TOXICITY STUDY OF AQUEOUS AND METHANOLIC EXTRACT

More information

Subcritical Water Extraction of Polyphenolic Compounds from Terminalia chebula Fruits

Subcritical Water Extraction of Polyphenolic Compounds from Terminalia chebula Fruits Chiang Mai J. Sci. 2008; 35(1) FU-003 103 Chiang Mai J. Sci. 2008; 35(1) : 103-108 www.science.cmu.ac.th/journal-science/josci.html Contributed Paper Subcritical Water Extraction of Polyphenolic Compounds

More information

PRELIMINARY PHYTOCHEMICAL STUDIES ON SOLANUM SURATTENSE BURM.F. SEEDS

PRELIMINARY PHYTOCHEMICAL STUDIES ON SOLANUM SURATTENSE BURM.F. SEEDS Ancient Science of Life Vol : XXVI (1&2) July, August, September, October, November, December 2006 PRELIMINARY PHYTOCHEMICAL STUDIES ON SOLANUM SURATTENSE BURM.F. SEEDS S. SHAHILADEVI, G. JAYANTHI AND

More information

Chandan Prasad.et.al. Int. Journal of Engineering Research and Application ISSN : , Vol. 7, Issue 9, ( Part -6) September 2017, pp.

Chandan Prasad.et.al. Int. Journal of Engineering Research and Application ISSN : , Vol. 7, Issue 9, ( Part -6) September 2017, pp. RESEARCH ARTICLE OPEN ACCESS Antibacterial properties of Unripened Fruits of Trapa natans, Annona squamosa, Ficus carica and Anthocephalus cadamba against Pathogenic Gram Positive and Negative Bacteria.

More information

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO FREE RADICAL SCAVENGING ACTIVITY OF MELOCHIA CORCHORIFOLIA PLANT EXTRACTS

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO FREE RADICAL SCAVENGING ACTIVITY OF MELOCHIA CORCHORIFOLIA PLANT EXTRACTS INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article PRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO FREE RADICAL SCAVENGING ACTIVITY OF MELOCHIA

More information

Keywords- Hibiscus rosa- sinensislinn., phytochemical, extract.antibacterial, axillary solitary, companulate.

Keywords- Hibiscus rosa- sinensislinn., phytochemical, extract.antibacterial, axillary solitary, companulate. GLOBAL JOURNAL OF ENGINEERING SCIENCE AND RESEARCHES COMPARATIVE PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF ETHANOLIC AND WATER FLOWER EXTRACTS OF HIBISCUS ROSASINENSIS LINN. (HIBISCUS RED)

More information

Journal home page: ESTIMATION OF NARINGIN AND HESPERIDIN FROM CITRUS FRUITS BY HPTLC

Journal home page:   ESTIMATION OF NARINGIN AND HESPERIDIN FROM CITRUS FRUITS BY HPTLC Page6175 Indo American Journal of Pharmaceutical Research, 2013 ISSN NO: 2231-6876 Journal home page: http:///index.php/en/ INDO AMERICAN JOURNAL OF PHARMACEUTICAL RESEARCH ESTIMATION OF NARINGIN AND HESPERIDIN

More information

PHARMACOGNOSTIC, PHYTOCHEMICAL STUDIES ON THE ROOT OF CLERODENDRON INFORTUNATUM LINN ROOT

PHARMACOGNOSTIC, PHYTOCHEMICAL STUDIES ON THE ROOT OF CLERODENDRON INFORTUNATUM LINN ROOT INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article PHARMACOGNOSTIC, PHYTOCHEMICAL STUDIES ON THE ROOT OF CLERODENDRON INFORTUNATUM LINN ROOT

More information

ANIMALS OILS AND FATS CHAPTER 2 PREPARATION AND SAPONIFICATION OF SPERMACETI 1. PREPARATION OF SPERMACETI

ANIMALS OILS AND FATS CHAPTER 2 PREPARATION AND SAPONIFICATION OF SPERMACETI 1. PREPARATION OF SPERMACETI 182 ANIMALS OILS AND FATS CHAPTER 2 PREPARATION AND SAPONIFICATION OF SPERMACETI 1. PREPARATION OF SPERMACETI 690. The spermaceti I examined was separated as follows from a yellow colored oil which commercial

More information

In vitro antimicrobial activity of leaves and bark extracts of Ficus religiosa (Linn.)

In vitro antimicrobial activity of leaves and bark extracts of Ficus religiosa (Linn.) INDIAN JOURNAL OF PHARMACEUTICAL & BIOLOGICAL RESEARCH (IJPBR). Vol. 1 (1), March., 13 In vitro antimicrobial activity of leaves and bark extracts of Ficus religiosa (Linn.) G.Ramakrishnaiah * 1, T. Hariprasad

More information

Botanical and phytochemical evaluation of fruits of spondias pinnata

Botanical and phytochemical evaluation of fruits of spondias pinnata Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2015, 7 (5):276-280 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4

More information

Journal of Chemical and Pharmaceutical Research, 2013, 5(3): Research Article

Journal of Chemical and Pharmaceutical Research, 2013, 5(3): Research Article Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2013, 5(3):98-104 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Preliminary phytochemical screening and HPTLC fingerprinting

More information

IJPRD, 2013; Vol 5(08): October-2013 ( ) International Standard Serial Number

IJPRD, 2013; Vol 5(08): October-2013 ( ) International Standard Serial Number IJPRD, 2013; Vol 5(08): October-2013 (001 006) International Standard Serial Number 0974 9446 --------------------------------------------------------------------------------------------------------------------------------------------------

More information

PRELIMINARY PHYSICO-PHYTOCHEMICAL STUDY OF THE FRUIT OF A MEDICINAL PLANT CASSIA FISTULA L.

PRELIMINARY PHYSICO-PHYTOCHEMICAL STUDY OF THE FRUIT OF A MEDICINAL PLANT CASSIA FISTULA L. Int. J. Chem. Sci.: 9(1), 2011, 223-228 ISSN 0972-768X PRELIMINARY PHYSICO-PHYTOCHEMICAL STUDY OF THE FRUIT OF A MEDICINAL PLANT CASSIA FISTULA L. NIDHI SAXENA, P. N. SHRIVASTAVA and R. C. SAXENA Department

More information

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO ANTIBACTERIAL ACTIVITY OF CUCURBITA MAXIMA SEED EXTRACT

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO ANTIBACTERIAL ACTIVITY OF CUCURBITA MAXIMA SEED EXTRACT INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article PRELIMINARY PHYTOCHEMICAL SCREENING AND INVITRO ANTIBACTERIAL ACTIVITY OF CUCURBITA MAXIMA

More information

Hawler Medical University College of Pharmacy Dept. of Pharmacognosy Course Book Pharmacognosy I and II, 3 rd year

Hawler Medical University College of Pharmacy Dept. of Pharmacognosy Course Book Pharmacognosy I and II, 3 rd year Hawler Medical University College of Pharmacy Dept. of Pharmacognosy Course Book Pharmacognosy and, 3 rd year Course Coordinator: Dr. Alaadin M. Naqishbandi Assistant Professor 014-015 Course Coordinator

More information

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF PAIN RELIEF HERBAL FORMULATIONS

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF PAIN RELIEF HERBAL FORMULATIONS Page5112 Indo American Journal of Pharmaceutical Research, 2016 ISSN NO: 2231-6876 ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF PAIN RELIEF HERBAL FORMULATIONS Rucha J. Shah 1, Ajay I. Patel 1, Kartik

More information

β-sitosterol-3-o-β-d-xylopyranosyl (1 4)-O-β-D- GLUCOPYRANOSIDE FROM THE SEEDS OF ZANTHOXYLUM HEMILTONIANUM WALL

β-sitosterol-3-o-β-d-xylopyranosyl (1 4)-O-β-D- GLUCOPYRANOSIDE FROM THE SEEDS OF ZANTHOXYLUM HEMILTONIANUM WALL Int. J. Chem. Sci.: 11(2), 201, 111-116 ISSN 0972-768X www.sadgurupublications.com β-sitsterl---β-d-xylpyransyl (1 4)--β-D- GLUCPYRANSIDE FRM TE SEEDS F ZANTXYLUM EMILTNIANUM WALL SANDYA RAJPT * Department

More information

CDI Mediated Monoacylation of Symmetrical Diamines and Selective Acylation of Primary Amines of Unsymmetrical Diamines

CDI Mediated Monoacylation of Symmetrical Diamines and Selective Acylation of Primary Amines of Unsymmetrical Diamines Supporting information: CDI Mediated Monoacylation of Symmetrical Diamines and Selective Acylation of Primary Amines of Unsymmetrical Diamines Sanjeev K. Verma*, Ramarao Ghorpade, Ajay Pratap and M. P.

More information

Evaluation of anti-arthritic potential of NONI extract in experimental rats

Evaluation of anti-arthritic potential of NONI extract in experimental rats World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.org/ Original

More information

Examination of Chemicals in Trap Cases. (Phenolphthalein)

Examination of Chemicals in Trap Cases. (Phenolphthalein) Introduction Examination of Chemicals in Trap Cases (Phenolphthalein) Although a number of different techniques using different chemicals such as fluorescent dyes, starch powder, phenolphthalein powders

More information

Isolation and characterization of stigmast- 5-en-3β-ol (β-sitosterol) from the leaves of Hygrophila spinosa T. Anders

Isolation and characterization of stigmast- 5-en-3β-ol (β-sitosterol) from the leaves of Hygrophila spinosa T. Anders Isolation and characterization of stigmast- 5-en-3β-ol (β-sitosterol) from the leaves of Hygrophila spinosa T. Anders Arjun Patra 1*, S. Jha 2, P.N. Murthy 3, Manik 2, A. Sharone 2 1 College of Pharmacy,

More information

Preliminary phytochemical screening of foliar extract of Anthocephalus cadamba (Roxb.)

Preliminary phytochemical screening of foliar extract of Anthocephalus cadamba (Roxb.) International Journal of Sciences & Applied Research www.ijsar.in Preliminary phytochemical screening of foliar extract of Anthocephalus cadamba (Roxb.) Basant Kumar 1, Garima Bartariya 2 and Abhishek

More information

aspera & Asteracantha longifolia

aspera & Asteracantha longifolia INTERNATIONAL JOURNAL OF ADVANCES IN PHARMACY, BIOLOGY AND CHEMISTRY Research Article Phytochemical Details of Glycine max, Achyranthes aspera & Asteracantha longifolia Safeena Sheikh*, Suhail Asghar,

More information

Extraction and Standardization of Medicinal Herbal Products

Extraction and Standardization of Medicinal Herbal Products Extraction and Standardization of Medicinal Herbal Products By: Dr Masoud Sadeghi Dinani Department of Pharmacognosy Isfahan University of Medical Sciences Medicinal Plants About 80% of all the world s

More information

International Journal of Food Nutrition and Safety, 2012, 1(2): International Journal of Food Nutrition and Safety

International Journal of Food Nutrition and Safety, 2012, 1(2): International Journal of Food Nutrition and Safety International Journal of Food Nutrition and Safety, 2012, 1(2): 54-59 International Journal of Food Nutrition and Safety Journal homepage: www.modernscientificpress.com/journals/ijfns.aspx ISSN: 2165-896X

More information

Preparation of Penicillins by Acylation of 6-Aminopenicillanic acid with Acyl Chlorides Week One: Synthesis

Preparation of Penicillins by Acylation of 6-Aminopenicillanic acid with Acyl Chlorides Week One: Synthesis Preparation of Penicillins by Acylation of 6-Aminopenicillanic acid with Acyl Chlorides Week One: Synthesis Wear gloves during this experiment. Dissolve 1.05g of sodium bicarbonate in 12mL of acetone:

More information

International Journal of Research in Pharmacology & Pharmacotherapeutics

International Journal of Research in Pharmacology & Pharmacotherapeutics International Journal of Research in Pharmacology & Pharmacotherapeutics ISSN Print: 2278-2648 IJRPP Vol.4 Issue 2 April-June-2015 ISSN Online: 2278-2656 Journal Home page: Research article Open Access

More information

International Journal of Pharma and Bio Sciences

International Journal of Pharma and Bio Sciences Research Article Microbiology International Journal of Pharma and Bio Sciences ISSN 0975-6299 PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF CLITORIA TERNATEA LEAVES AGAINST STAPHYLOCOCCUS AUREUS

More information

TECHNICAL METHODS GASTRIC CONTENTS AND URINE THE IDENTIFICATION OF BARBITURATE DRUGS IN. aspirated and examined as well.

TECHNICAL METHODS GASTRIC CONTENTS AND URINE THE IDENTIFICATION OF BARBITURATE DRUGS IN. aspirated and examined as well. J. clin. Path. (1950), 3, 152. TECHNICAL METHODS THE IDENTIFICATION OF BARBITURATE DRUGS IN GASTRIC CONTENTS AND URINE BY From the Pathological Depariment, Salisbury General Infirmary (RECEIVED FOR PUBLICATION

More information

Journal of Chemical and Pharmaceutical Research, 2013, 5(1): Research Article

Journal of Chemical and Pharmaceutical Research, 2013, 5(1): Research Article Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2013, 5(1):180-184 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 A simple and sensitive RP-HPLC method for estimation

More information

Antioxidant Activity of Phytoconstituents Isolated from Leaves of Tridax procumbens

Antioxidant Activity of Phytoconstituents Isolated from Leaves of Tridax procumbens International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.5, No.4, pp 1640-1644, Oct-Dec 2013 Antioxidant Activity of Phytoconstituents Isolated from Leaves of Tridax procumbens

More information

Supporting Information. for. Pd-catalyzed decarboxylative Heck vinylation of. 2-nitro-benzoates in the presence of CuF 2

Supporting Information. for. Pd-catalyzed decarboxylative Heck vinylation of. 2-nitro-benzoates in the presence of CuF 2 Supporting Information for Pd-catalyzed decarboxylative Heck vinylation of 2-nitro-benzoates in the presence of CuF 2 Lukas J. Gooßen*, Bettina Zimmermann, Thomas Knauber Address: Department of Chemistry,

More information

INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES

INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES Development of standardization of trikatu churna: A spectrophotometric approach Manjesh Thakur*, P. Porwal, P. Pal, K. Shukla and S.C. Mahajan Mahakal

More information

Formulation and Evaluation by Phytochemical Analysis of Herbal Ointment

Formulation and Evaluation by Phytochemical Analysis of Herbal Ointment Human Journals Research Article February 2018 Vol.:8, Issue:4 All rights are reserved by ROHIT JAYSING BHOR et al. Formulation and Evaluation by Phytochemical Analysis of Herbal Ointment Keywords: Herbal

More information

Phytochemical and Physico-Chemical Analysis of Siddha Preparation Magizham Pattai Chooranam

Phytochemical and Physico-Chemical Analysis of Siddha Preparation Magizham Pattai Chooranam International Journal of Research in Pharmacy and Biosciences Volume 1, Issue 2, December 2014, PP 11-15 ISSN 2394-5885 (Print) & ISSN 2394-5893 (Online) Phytochemical and Physico-Chemical Analysis of

More information

Supporting Information. Efficient copper-catalyzed Michael addition of acrylic derivatives with primary alcohols in the presence of base

Supporting Information. Efficient copper-catalyzed Michael addition of acrylic derivatives with primary alcohols in the presence of base Supporting Information Efficient copper-catalyzed Michael addition of acrylic derivatives with primary alcohols in the presence of base Feng Wang, a Haijun Yang, b Hua Fu, b,c * and Zhichao Pei a * a College

More information