IR-4 Ornamental Horticulture Program Research Report Cover Sheet

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1 q.pdf IR4 Ornamental Horticulture Program Research Report over Sheet Researcher(s) William Kirk Trial: 2015 MI Affiliation: Michigan State University PhoneNumber: Lansing MI ProjectTitle: Leaf Spot & Anthracnose Efficacy Protocol #: PR# Research Target rop/plant Product EPA Reg. # Production Site Status Leaf Spot oneflower, Orange F9110 F9110 FM Leaf Spot oneflower, Orange MBI 110 MBI110 Marrone Bio In Leaf Spot oneflower, Orange Medallion Fludioxonil Syngenta Leaf Spot oneflower, Orange NUP fludioxonil Nufarm Leaf Spot oneflower, Orange Orkestra Intrinsic (BAS703 0 Fluxapyroxad + pyraclostrobi BASF Leaf Spot oneflower, Orange Proud 3 Thyme oil (5.6%) Biohumanetics Leaf Spot oneflower, Orange S2200 4S Mandestrobin Valent

2 Project Title: IR4 Ornamental Horticulture Program Efficacy of Management Tools for Fungal Leaf Spots and Anthracnose Protocol #: Narrative Summary (Results/Discussion) Please keep text to one page if possible. Include summary of trial results and a brief discussion. Results for multiple PRnumbers can be summarized together, but please list all PRNumbers in the footer and in the summary data table. General The Rudbeckia (Rudbeckia fulgida cv. Goldsturm) obtained for this trial were known to be susceptible to anthracnose leaf spot. Phytotoxicity was not noted in any treatment after any of the applications. Alternaria leaf spot developed slowly but steadily as extended leaf wetness periods did not occur through the summer of 2015 and only moderate air temperature occurred during Aug and untreated controls reached an average of about 20% foliar infection by 2 Sep. Up to 21 Aug, most fungicide programs had significantly less foliar leaf spot than the untreated control. By 21 Aug, all programs had significantly less Alternaria leaf spot than the untreated control (11.1 %). By 2 Sep, all programs had significantly less Alternaria leaf spot than the untreated control (20.8 %) and programs with 5.6 to 8.0; 6.0 to to 10.4 and % Alternaria leaf spot were not significantly different from each other. All fungicide programs had lower RAUDP values in comparison to the untreated control (5.1). Programs with RAUDP values of 1.2, 1.6 to 1.9, 1.9 to 2.3, 2.3 to 2.8, 2.5 to 3.0 and 3.9 were not significantly different from each other. 1

3 IR4 Ornamental Horticulture Program Results Table 1 Please insert results table here. Include PRnumbers for each treatment if multiple PRnumbers are included in this summary. Please include statistics. Treatment and rate of application per water 7/31 (58 DAT a ) Alternaria Leaf Spot (%) 8/7 (65 DAT) 8/21 (79 DAT) 9/2 (91 DAT) RAUDP b 33 DAI 1 BAS F L 8 fl oz (ABE c ) 0.0 d d 1.2 f 2.0 e 5.6 e 1.2 g 2 F9110 WG 24 oz wt (ABDE) 1.3 ab 1.9 cde 5.0 c 7.6 cde 2.5 cd 3 MBI L 1 gal (ABDE) 1.5 a 3.6 a 7.1 b 13.6 b 3.9 b 4 NUP L 4 fl oz (AE) 0.8 bc 1.2 f 5.0 c 12.3 b 2.6 cd 5 NUP L 8 fl oz (AE) 0.8 bc 1.7 def 5.0 c 10.4 bc 2.6 cd 6 Proud 3 100L 4 qt (ABDE) 1.3 ab 3.0 ab 6.3 bc 8.0 cde 3.0 c 7 S S 7.5 fl oz (AE) 0.8 bc 2.5 bcd 5.0 c 6.0 de 2.3 de 8 S S 15 fl oz (AE) 1.3 ab 2.5 bcd 5.6 bc 8.5 cd 2.8 cd 9 Pageant 38WG 14 oz wt (AE) 0.3 cd 1.4 ef 3.1 d 6.7 de 1.6 f 10 Medallion 50WG 8 oz wt (AE) 0.8 bc 1.7 def 3.4 d 7.1 de 1.9 ef 11 Untreated heck 1.5 a 2.7 abc 11.1 a 20.8 a 5.1 a a DAP = days after transplanting on 3 Jun b RAUDP, relative area under the disease progress curve calculated from day of appearance of initial symptoms (31 Jul) to 2 Sep (33 days). c dates for treatments; A= 3 Jul; B= 10 Jul; = 17 Jul; D 24 Jul; E= 31 Jul. d Values followed by the same letter are not significantly different at p = 0.05 (Fishers LSD) after back transformation from arcsine square root percent transformation to normalize data. 2

4 IR4 Ornamental Horticulture Program Materials & Methods/Recordkeeping Please fill out the information below or attach a separate document with comparable information. rop History rop ultivar/variety: Date of Seeding: Date of Emergence: Rudbeckia (Rudbeckia fulgida cv. Goldsturm). Date of Transplanting: 6/3/2015 Potting Mix: Field grown apac loam soil Pot size & spacing: 12 between plant centers 3 feet between rows. Plants transplanted into soil. Row spacing: 1 ft between plants Product(s) applied prior to start of experiment: Product Rate Type Date of rop Growth Stage Volume Add more rows as needed. Experiment Information Experimental Design: RB Number of Reps: 4 Materials & Methods: One location was used, at larksville Horticultural Research Farm, Michigan State University. Plants were treated during Jun as described below. Plants were produced from seeds by Raker seeds, oldwater, MI, and then transplanted by hand on 6/3/2015. Plants were situated in rows 12 apart within single rows (36 apart). Each treatment was replicated four times with plots consisting of 10 plants for a total of 40 plants per treatment. All fungicides in this trial were applied from 3 Jul to 31 Jul with a hand held R&D spray boom calibrated to deliver /A (80 p.s.i.). 3

5 IR4 Ornamental Horticulture Program Product(s) applied during experiment (including treatments, fertilizers, etc): Product BAS F L F9110 WG 24 oz MBI L NUP L NUP L Proud 3 100L S S S S Pageant 38WG Medallion 50WG Rate(s) Per Type Date of rop Growth Stage 8 oz Fungicide 3, 10, 24, 31 Jul 4, 6, 8, 12 leaf 24 oz Fungicide 3, 10, 17, 24, 31 4, 6, 8, 10, 12 Jul leaf 1 gal Fungicide 3, 10, 17, 24, 31 4, 6, 8, 10, 12 Jul leaf 4 fl oz Fungicide 3, 17, 31 Jul 4, 8, 12 leaf 8 fl oz Fungicide 3, 17, 31 Jul 4, 8, 12 leaf 4 qt Fungicide 3, 10, 17, 24, 31 4, 6, 8, 10, 12 Jul leaf 7.5 fl oz Fungicide 3, 17, 31 Jul 4, 8, 12 leaf 15 fl oz Fungicide 3, 17, 31 Jul 4, 8, 12 leaf 14 oz Fungicide 3, 17, 31 Jul 4, 8, 12 leaf 8 oz Fungicide 3, 17, 31 Jul 4, 8, 12 leaf Volume /A Add more rows as needed. 4

6 IR4 Ornamental Horticulture Program Environmental conditions during the experiment: Insert temperature, precipitation, relative humidity and other pertinent environmental conditions. Or send separate file with this information. Meteorological variables were measured with a ampbell weather station located at the farm from 1 May to 31 Oct. Average daily air temperature ( o F) from Jun to Oct was 59.3, 63.8, 68.3, 67.3, 64.7 and 49.9 and the number of days with maximum temperature >90 o F was 0 (May to Oct). Average daily relative humidity (%) over the same period was 69.4, 73.1, 70.5, 74.1, 71.8 and Average daily soil temperature at 4 depth ( o F) over the same period was 60.0, 68.2, 73.6, 71.6, 68.9 and Average daily soil moisture at 4 depth (% of field capacity) over the same period was 35.6, 36.6, 32.7, 35.7, 37.1, and Precipitation was 4.01, 3.02, 0.29, 3.09, 3.52, and 3.07 in. Plots were irrigated to supplement precipitation to about 0.1 in./4 day period with overhead sprinkle irrigation. Data ollected Please describe data collected and scoring system. Also include the dates data were collected. Plots were rated for Alternaria leaf spot 28, 35, 49 and 61 days after planting. The Relative Area Under the Disease Progress urve (RAUEP) was calculated 33 days after the initial appearance of leaf spot on 31 Jul. Raw Data Insert raw data below or send separate file containing raw data. Raw data file attached 5

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